- Title
- Moringa oleifera leaf powder as a functional antioxidant additive in pork droewors
- Creator
- Mukumbo, Felicitas Esnart
- Subject
- Antioxidants
- Subject
- Moringa oleifera
- Subject
- Pork -- Quality
- Date
- 2016
- Type
- Thesis
- Type
- Doctoral
- Type
- PhD
- Identifier
- http://hdl.handle.net/10353/15354
- Identifier
- vital:40400
- Description
- The study investigated the effect of Moringa oleifera leaf powder (MLP) on physico-chemical characteristics, antioxidant activity, antioxidant compound content and lipid oxidation in pork droëwors. Firstly, the physico-chemical properties (proximate composition, salt content, water activity (aw), pH) of commercially produced droëwors from different types of meat (beef, game, ostrich) were determined. In the second experiment beef and pork droëwors with similar fat content were produced. Physico-chemical properties and lipid oxidation (thiobarbituric reactive substances (TBARS)) during processing and 26 days of storage at 25 °C and 50percent relative humidity (RH) were measured. In the third experiment, antioxidant compounds (Total Phenolic Compounds (TPC), α-tocopherol, β-carotene) in MLP were quantified. Thereafter, 4 treatments of pork droëwors were produced, with MLP included at 0, 0.5, 1 and 2 g/ 100 g. Physico-chemical properties and TBARS were measured at intervals during drying (0, 1.5, 5.75, 27.25, 72 h) and after 7 days of storage under ambient conditions. Antioxidant activity (ferric reducing antioxidant power (FRAP)), TBARS, α- and γ-tocopherol, and β-carotene contents were measured. In the fourth experiment, three batches of droëwors were produced (C: no antioxidant, M0.75: 0.75 g/ 100 g MLP, VE: 15 mg/ kg α-tocopherol oil) and stored at 25 °C and 50percent relative humidity for 112 days. Drying kinetics and α-tocopherol contents of pork droëwors after drying were measured and the physico-chemical properties and TBARS were followed during storage. Results showed no differences (P > 0.05) in the physico-chemical characteristics of beef, game meat and ostrich droëwors; containing on average 25.8 ± 1.25 g/100 g moisture, 42.0 ± 0.10 g/100 g protein, 32.0 ± 1.68 g/100 g fat, 6.2 ± 0.13 g/100 g ash and 4.2 ± 0.10 g/100 g salt; with aw and pH of 0.79 ± 0.015 and 5.3 ± 0.05, respectively. During processing and storage, TBARS were higher (P < 0.05) in pork droëwors (maximum 3.83 mg MDA/kg DM) than in beef (maximum 0.99 mg MDA/kg DM). Moringa oleifera leaf powder contained high levels of TPC (7.5 ± 0.2 mg gallic acid eq/g) and substantial levels of α-tocopherol (76.7 ± 1.9 mg/100 g) and β-carotene (23.2 ± 2.8 mg/100 g). The FRAP, α-tocopherol and β-carotene content of pork droëwors increased (P < 0.05) proportionally with increasing levels of MLP inclusion. Lipid oxidation occurred more rapidly (P < 0.05) when MLP was not added and was similar (P > 0.05) for all MLP treatments. There was no significant effect of the inclusion of 0.75 g/100 g MLP on the drying curves and physico-chemical characteristics of the droëwors. The α-tocopherol content was higher (P < 0.05) and TBARS during storage were lower (P > 0.05) with MLP addition. The results of the current study give an overview of the composition of commercial droëwors and showed higher susceptibility to lipid oxidation in pork droëwors. Moringa oleifera leaf powder exhibited antioxidant activity in pork droëwors, inhibited lipid oxidation and increased the content of α-tocopherol in the product. It can be concluded that MLP could be used as a functional antioxidant additive in pork droëwors.
- Format
- 136 leaves
- Format
- Publisher
- University of Fort Hare
- Publisher
- Faculty of Science and Agriculture
- Language
- English
- Rights
- University of Fort Hare
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