Effect of dietary Mucuna pruriens seed meal on the serum lipid profile and bone quality traits of broiler chickens
- Authors: Hempe, Chumani
- Date: 2019
- Subjects: Legumes Lipoproteins
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/17213 , vital:40866
- Description: This study aimed at evaluating the effects of heated Mucuna pruriens seed meal on the blood serum lipid profile and bone quality parameters of broiler chickens. A total of 120 day-old unsexed Cobb 500 broiler chicks were randomly allocated for the 4 treatment diets (T1, T2, T3 and T4) containing 0, 10, 15, and 20% heated Mucuna pruriens seed meal. The birds were reared on wood shaving floor pens for 35 days. Birds were humanely slaughtered by severing the jugular vein using a sharp knife. Blood was collected into a tube, centrifuge and analyzed for a lipid profile analysis (total cholesterol (TC), high density lipoproteins (HDL), low density lipoproteins (LDL), triglycerides (TCD) as well as aspartate transaminase (AST) and alanine transaminase (ALT)) by enzymatic diagnostic kits (Diasys diagnostic kits). Both drumsticks severed using a sharp knife. After boiling in the water at 100°C for 10 mins the tibia bones were taken, air-dried for 96 hrs at room temperature and measured for weights. Bone length, strength, moisture and ash content as well as morphology were measured. The blood lipid profile of birds fed T1 was high (P < 0.05) for all the parameters measured, while T3 had the lowest (P < 0.05) profile. T2 and T4 had the same profile. ALT was found to be highest (P < 0.05) for T4 (5.27 ± 0.207/L) and lowest (P < 0.05) for T2 (4.67 ± 0.333/L) while AST was highest (P < 0.05) for T2 (320.13 ± 28.851/L) and lowest (P < 0.05) for T3 (208.47 ± 10.059/L). Treatment diet 3 (T3) was observed decreased bone quality among all diets; with the lowest (P < 0.05) bone strength (188.55 ± 12.039N), bone length (8.59 ± 0.131cm) and morphology (1.69 ± 0.058). Mucuna pruriens seed meal inclusion in broiler diets reduces blood serum lipids and 20% inclusion levels have no adverse effects on the bone quality attributes
- Full Text:
- Authors: Hempe, Chumani
- Date: 2019
- Subjects: Legumes Lipoproteins
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/17213 , vital:40866
- Description: This study aimed at evaluating the effects of heated Mucuna pruriens seed meal on the blood serum lipid profile and bone quality parameters of broiler chickens. A total of 120 day-old unsexed Cobb 500 broiler chicks were randomly allocated for the 4 treatment diets (T1, T2, T3 and T4) containing 0, 10, 15, and 20% heated Mucuna pruriens seed meal. The birds were reared on wood shaving floor pens for 35 days. Birds were humanely slaughtered by severing the jugular vein using a sharp knife. Blood was collected into a tube, centrifuge and analyzed for a lipid profile analysis (total cholesterol (TC), high density lipoproteins (HDL), low density lipoproteins (LDL), triglycerides (TCD) as well as aspartate transaminase (AST) and alanine transaminase (ALT)) by enzymatic diagnostic kits (Diasys diagnostic kits). Both drumsticks severed using a sharp knife. After boiling in the water at 100°C for 10 mins the tibia bones were taken, air-dried for 96 hrs at room temperature and measured for weights. Bone length, strength, moisture and ash content as well as morphology were measured. The blood lipid profile of birds fed T1 was high (P < 0.05) for all the parameters measured, while T3 had the lowest (P < 0.05) profile. T2 and T4 had the same profile. ALT was found to be highest (P < 0.05) for T4 (5.27 ± 0.207/L) and lowest (P < 0.05) for T2 (4.67 ± 0.333/L) while AST was highest (P < 0.05) for T2 (320.13 ± 28.851/L) and lowest (P < 0.05) for T3 (208.47 ± 10.059/L). Treatment diet 3 (T3) was observed decreased bone quality among all diets; with the lowest (P < 0.05) bone strength (188.55 ± 12.039N), bone length (8.59 ± 0.131cm) and morphology (1.69 ± 0.058). Mucuna pruriens seed meal inclusion in broiler diets reduces blood serum lipids and 20% inclusion levels have no adverse effects on the bone quality attributes
- Full Text:
The effect of replacing antibiotic growth promoters with moringa oleifera leaf powder on growth performance, carcass characteristics, immune organ indices, gut microflora, physicochemical and sensory quality of broiler meat
- Nantapo, Carlos Wyson Tawanda
- Authors: Nantapo, Carlos Wyson Tawanda
- Date: 2018
- Subjects: Moringa oleifera Meat--Quality Broilers (Chickens)
- Language: English
- Type: Thesis , Doctoral , Animal Science
- Identifier: http://hdl.handle.net/10353/17749 , vital:41197
- Description: The objective of the study was to determine the effect of feeding graded levels of Moringa oleifera leaf meal powder (MOLP) on growth performance, carcass characteristics, economic analysis, health status, physicochemical, sensory properties, mineral composition and retention values of meat. A total of 1600 Comb-500 day-old unsexed chicks were allotted to 4 dietary treatment groups, with 4 replicate pens of 100 birds each in a completely randomised design. The dietary treatments were basal diets supplemented with different levels of MOLP as follows; Treatment 1/ control: Basal diet with antibiotic Zinc bacitracin and coccidiostatic Maxiban and no MOLP (0MOLP); Treatment 2: Basal diet included 1percent MOLP (1MOLP); Treatment 3: Basal diet included 3.0 percent MOLP (3MOLP); Treatment 4: Basal diet included 5 percent MOLP (5MOLP). Birds fed basal diets plus 5percent MOLP at starter phase achieved the least FCR 1.53 (P < 0.037), whilst consuming significantly less feed ADFI 47.4 g (P < 0.05). For the entire feeding period, birds fed 5 percent MOLP achieved a favourable FCR of 1.80 among all treatment groups (P < 0.001), but achieved the lowest final live weight (1416 g), carcass weights (1044g) (P < 0.05). Birds fed 0 percent MOLP recorded highest final weight, carcass weight (1339) and the best dressing percentages (76.8percent) (P < 0.05). Abdominal fat levels were reduced by elevating MOLP in diets (P < 0.05). Improvements in proportions of giblets, gizzard, liver and intestine were observed with increase in dietary MOLP. Feed costs increased, while gross and net returns and economic efficiency decreased as dietary MOLP increased. Organ indices of spleen, Bursa of Fabricius and liver were significantly increased by 5percent MOLP in the diet (P < 0.05). Macroscopic lesions scores (P < 0.05) were significantly higher in duodenum, jejunum and ileum of birds in the 1MOLP treatment group on d 28 and d 35. Control and 5MOLP groups recorded the least bloody excreta scores among all treatment groups (P < 0.05). Highest total aerobic bacteria (TAB), E. coli, C. perfringens counts and the lowest lactobacillus spp counts were observed in all intestinal segments in birds in the 1MOLP treatment group. Lower E. coli and C. perfringens but higher lactobacillus spp counts were observed in control and 5MOLP groups (P < 0.05) in the duodenum and jejunum. Increasing MOLP levels in diets led to lower pH and pHu in both breast and leg muscles (P < 0.05). pH was consistently lower in leg muscles than in breast cuts in all treatments and a large decline in post-mortem pH was observed in all muscles of birds in control group. A dietary increase in MOLP level led to a significant increase in L* and b* (P < 0.05) negative correlation between pH and L* (r = -0.463) (P < 0.05) and a weak but significant (P < 0.001) correlation (r = 0.176) between pH and drip loss. Herb flavour (P = 0.0029), appearance (P = 0.0156), aroma (P = 0.0011), colour (P = 0.0375), taste (P = 0.0032) and overall acceptability (P = 0.0192) were improved by grilling, microwave cooking and pan-frying in olive oil. These changes were more pronounced in breast muscle, thus following the trend; microwave > pan-frying in olive oil > grilled samples > boiling for best cooked product based on overall acceptability. Grilling and frying heart muscles resulted in the highest Ca (45.9 mg/kg) and Mg (98.9 mg/kg), whereas boiling led to the lowest Ca (41.1 mg/kg) and Mg (91.4 mg/kg) concentration. The highest concentrations of Fe and Zn these minerals were recorded in grilled (32.7 mg/kg) and fried samples (75.2 mg/kg). Zinc and Fe concentration observed in liver and heart muscles were higher than those found in breast and leg muscle. In conclusion, the results confirm the potential of MOLP for use as an alternative to antibiotics in maintaining broiler health, reducing mortalities and improving carcass characteristics and some meat quality traits.
- Full Text:
- Authors: Nantapo, Carlos Wyson Tawanda
- Date: 2018
- Subjects: Moringa oleifera Meat--Quality Broilers (Chickens)
- Language: English
- Type: Thesis , Doctoral , Animal Science
- Identifier: http://hdl.handle.net/10353/17749 , vital:41197
- Description: The objective of the study was to determine the effect of feeding graded levels of Moringa oleifera leaf meal powder (MOLP) on growth performance, carcass characteristics, economic analysis, health status, physicochemical, sensory properties, mineral composition and retention values of meat. A total of 1600 Comb-500 day-old unsexed chicks were allotted to 4 dietary treatment groups, with 4 replicate pens of 100 birds each in a completely randomised design. The dietary treatments were basal diets supplemented with different levels of MOLP as follows; Treatment 1/ control: Basal diet with antibiotic Zinc bacitracin and coccidiostatic Maxiban and no MOLP (0MOLP); Treatment 2: Basal diet included 1percent MOLP (1MOLP); Treatment 3: Basal diet included 3.0 percent MOLP (3MOLP); Treatment 4: Basal diet included 5 percent MOLP (5MOLP). Birds fed basal diets plus 5percent MOLP at starter phase achieved the least FCR 1.53 (P < 0.037), whilst consuming significantly less feed ADFI 47.4 g (P < 0.05). For the entire feeding period, birds fed 5 percent MOLP achieved a favourable FCR of 1.80 among all treatment groups (P < 0.001), but achieved the lowest final live weight (1416 g), carcass weights (1044g) (P < 0.05). Birds fed 0 percent MOLP recorded highest final weight, carcass weight (1339) and the best dressing percentages (76.8percent) (P < 0.05). Abdominal fat levels were reduced by elevating MOLP in diets (P < 0.05). Improvements in proportions of giblets, gizzard, liver and intestine were observed with increase in dietary MOLP. Feed costs increased, while gross and net returns and economic efficiency decreased as dietary MOLP increased. Organ indices of spleen, Bursa of Fabricius and liver were significantly increased by 5percent MOLP in the diet (P < 0.05). Macroscopic lesions scores (P < 0.05) were significantly higher in duodenum, jejunum and ileum of birds in the 1MOLP treatment group on d 28 and d 35. Control and 5MOLP groups recorded the least bloody excreta scores among all treatment groups (P < 0.05). Highest total aerobic bacteria (TAB), E. coli, C. perfringens counts and the lowest lactobacillus spp counts were observed in all intestinal segments in birds in the 1MOLP treatment group. Lower E. coli and C. perfringens but higher lactobacillus spp counts were observed in control and 5MOLP groups (P < 0.05) in the duodenum and jejunum. Increasing MOLP levels in diets led to lower pH and pHu in both breast and leg muscles (P < 0.05). pH was consistently lower in leg muscles than in breast cuts in all treatments and a large decline in post-mortem pH was observed in all muscles of birds in control group. A dietary increase in MOLP level led to a significant increase in L* and b* (P < 0.05) negative correlation between pH and L* (r = -0.463) (P < 0.05) and a weak but significant (P < 0.001) correlation (r = 0.176) between pH and drip loss. Herb flavour (P = 0.0029), appearance (P = 0.0156), aroma (P = 0.0011), colour (P = 0.0375), taste (P = 0.0032) and overall acceptability (P = 0.0192) were improved by grilling, microwave cooking and pan-frying in olive oil. These changes were more pronounced in breast muscle, thus following the trend; microwave > pan-frying in olive oil > grilled samples > boiling for best cooked product based on overall acceptability. Grilling and frying heart muscles resulted in the highest Ca (45.9 mg/kg) and Mg (98.9 mg/kg), whereas boiling led to the lowest Ca (41.1 mg/kg) and Mg (91.4 mg/kg) concentration. The highest concentrations of Fe and Zn these minerals were recorded in grilled (32.7 mg/kg) and fried samples (75.2 mg/kg). Zinc and Fe concentration observed in liver and heart muscles were higher than those found in breast and leg muscle. In conclusion, the results confirm the potential of MOLP for use as an alternative to antibiotics in maintaining broiler health, reducing mortalities and improving carcass characteristics and some meat quality traits.
- Full Text:
Effects of Vigna unguiculata and Lablab purpureus on methanogenesis, haematological parameters and the quality of meat from Xhosa Lop Ear goats
- Authors: Washaya, Soul
- Date: 2017
- Subjects: Legumes Forage Forage plants
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10353/9996 , vital:35249
- Description: The objective of the current study was to evaluate the nutritive value of forage legumes Lablab purpureus (Lablab) and Vigna unguiculata (Cowpea) and their effects on intake, growth, blood parameters and meat quality in Xhosa goats. Forages were established at UFH research farm in the summer of the 2014 /15 growing season in blocks measuring 8m width x 16m long for each legume, replicated three times, making a total of four blocks, and eight treatment units for each legume. Samples were collected at three different stages of growth of preanthesis, anthesis and post anthesis for biomass yield determination. A homogenous sub-sample was then oven dried for 72 hours at 60 0C. After drying the forage samples were milled through a 1mm screen and a 20g sample from each stage of growth and forage sample was subjected to proximate analysis, in-vitro digestibility and anti-nutrient evaluation. The in-vitro digestibility experiment was done with rumen liquor of cattle and sheep collected from an abbatoir. Tannins, total phenolics and sapponins were the only anti-nutrients evaluated as they are perceived to influence methanogenesis in ruminat animals. At 50percent flowering, forage legumes were harvested for hay making which was then used in an in-vivo trial to evaluate the nutritive value of forages. This trial was conducted using eighteen one-year-old goats of average weight 14.2±0.24 kg over a period of sixty days. The results showed that biomass yield, which increased with stage of growth, was significantly higher (P < 0.05) for Lablab than Cowpea (7.968t/ha vs 7.105t/ha). Cowpea exhibited higher moisture content (7.72percent), Ash (13.11percent), acid detergent fibre (ADF) (38.42percent) and crude protein (CP) (20.23percent) than Lablab which had 7.50percent, 11.45 percent, 36.17percent and 19percent, respectively. Lablab forage had significantly higher (P < 0.05) fat content (2.41percent), neutral detergent fibre (NDF) (49.27percent) and hemicellulose (13.07percent) than Cowpea which had 2.1percent; 46.91percent and 8.48percent, respectively. The stage of growth significantly (P < 0.05) affected the mineral content of both forages. Lablab showed significantly higher (P < 0.05) values (1.22percent 0.55percent, 2.67percent) than Cowpea (1.00percent, 0.45percent, 2.30percent) for calcium (Ca), phosphorus (P), and potassium (K) respectively. Lablab forage had significantly higher (P < 0.05) tannin (3.345mgCAE/gDM), phenolic (13.47mgGAE/gDM) and saponin (0.75percent) content compared to Cowpea with values of 1.494mgCAEg/DM; 9.402mgGAE/gDM and vs 1.55percent respectively. The tannin content significantly influenced in-vitro digestibility and enteric methane production. The forage type, harvesting stage and inoculum source significantly (P < 0.05) affected the in-vitro digestibility of the forages. The in-vitro dry matter (IVDM) and in-vitro organic matter (IVOM) digestibility was significantly higher (P < 0.05) for Lablab (41.13percent; 39.63percent) than Cowpea (39.86percent; 19.44percent), respectively. Both forages recorded higher dry matter (DM) digestibility coefficients at anthesis stage of growth 61.58percent, and 51.5 percent for Lablab and Cowpea respectively. The IVOM digestibility increased with stage of harvest (23.7percent to 25.57percent) for Cowpea while Lablab showed a decrease (44.6 percent to 43.9percent) when cattle inoculum was used. The IVDM digestibility was higher (P < 0.05) for Lablab (50.5percent) when cattle inoculum was used while Cowpea was observed to have higher digestibility co-efficiencies (38.54percent) when sheep inoculum was used. Cowpea had lower (P < 0.05) IVOM digestibility than Lablab (19.44percent) vs. 39.6percent). Lablab exhibited higher (P < 0.05) metabolisable energy (ME) values than Cowpea (6.21 vs. 2.70 MJ/kgDM). In the feeding study, the total DM intake by goats for Lablab was higher (P < 0.05) compared to Cowpea (688.1 vs. 607.8 g/head/ day). There was a positive (P < 0.05) correlation between dry matter intake and average daily gain (r= 0.8513, and r=0.5279) for Cowpea and Lablab respectively. Lablab and Cowpea promoted growth of Xhosa goats at a rate of 35.0g/d; and 38.3g/d, respectively which was not significantly different (P > 0.05). However, this growth was associated with a significantly higher (P < 0.05) food conversion ratio (FCR) of 19.16 vs 18.11 for Cowpea and Lablab, respectively. The apparent DM, non structural carbohydrate (NSC) nutrient digestibilities were significantly different (P < 0.05) for Lablab and Cowpea. Furthermore sex of animal influenced significantly (P < 0.05) the DM, ADF, NSC and NDF digestibilities. Lablab and Cowpea did not influence (P > 0.05) individual VFA molar concentrations in both sexes. However, animals fed Lablab had significantly higher butyrate concentrations (P < 0.05). Lablab and Cowpea forages significantly lowered (P > 0.05) enteric methane production in goats compared to a concentrate diet, and this was related to animal activity. Animals produced significantly more (P < 0.05) methane gas when ruminating than feeding or just standing. The average methane produced each year was lower but not significant (P > 0.05) for animals fed Cowpea than Lablab (4.602kg CH4, vs. 4.719kg CH4) when expressed per year. Male animals produced more gas than females with values of 17.40L/day; 12.46 g/kg DMI and 0.126g/day vs. 15.47L/day, 12.28 g/kg DMI, and 0.0109g/day, respectively. Forage legumes had no significant effects (P > 0.05) on serum total proteins (TP), globulin, urea, creatinine, alanine aminotransferase (ALT), alkaline phosphatase (ALP), and gamma- glutamyl transferase (GGT) concentrations, but serum concentration of albumin was influenced by the diets (P < 0.05). Sex of animals significantly influenced ALP concentration (P < 0.05) with male animals exhibiting higer ALP values than females. Measured over time, the plasma concentration of urea and ALP significantly increased (P < 0.05) while creatinine and cholesterol concentration decreased (P < 0.05). Although the overall serum concentration of urea, total proteins, globulin, creatinine, ALT, ALP and GGT remained unchanged among the treatments and between sexes; treatment by sex, treatment by day and sex by day interactions were significant (P < 0.05). The white blood cell (WBC), red blood cell (RBC), mean corpuscular haemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC), haemoglobin, haematocrite, platelet and mean platelet volume (MPV) levels were similar among treatments and between sexes (P > 0.05). The WBC decreased with time by 8.07 x10 9/l while mean crepuscular volume (MCV) increased by 11.83(fl) from day 15 to day 55. Treatment diets significantly (P < 0.05) influenced neutrophile (percent) content while sex influenced basophile (percent) content. There were no treatment or sex effects (P > 0.05) on lymphocytes (percent), monocytes (percent) and eosinophiles (percent). In the meat quality study, the dietary treatments and sex had a significant (P < 0.05) effect on dressing out percent (DPpercent), initial pH, redness (a*), yellowness (b*), Warner Blartze Shear force (WBS) , drip loss and cooking loss in the chevon from Xhosa goats. Compared with a commercial pellet diet, Lablab and Cowpea diets resulted in similar slaughter body weights (SBW), hot carcass weight (HCW) and cold dressed mass (CDM) of carcasses. However, female animals exhibited a significantly (P < 0.05) higher DPpercent than male animals (42.3percent vs. 38.9percent) for all treatments. The male animals exhibited significantly (P < 0.05) higher pH values one hour after slaughter than females (5.79 vs 5.67). Neverthreless the diet and gender did not significantly (P > 0.05) affect the ultimate pH (pHu). Meat samples from male animals had significantly (P < 0.05) higher redness values than females. Feeding cowpea significantly (P < 0.05) increased meat redness with the least drip loss of chevon. Female animals produced significantly (P < 0.05) tougher chevon compared to male animals; 4.16kg and 3.36kg for female and male, respectively. Lablab and Cowpea posses high CP levels and moderately lower NDF with high ADF values that influences dry matter intake (DMI), digestibility and growth of goats. These forage legumes meet animal requirements for fat, ADF, NDF, CP, Ca, Mn, zinc and Cu and their biomass yield is satisfactory.
- Full Text:
- Authors: Washaya, Soul
- Date: 2017
- Subjects: Legumes Forage Forage plants
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10353/9996 , vital:35249
- Description: The objective of the current study was to evaluate the nutritive value of forage legumes Lablab purpureus (Lablab) and Vigna unguiculata (Cowpea) and their effects on intake, growth, blood parameters and meat quality in Xhosa goats. Forages were established at UFH research farm in the summer of the 2014 /15 growing season in blocks measuring 8m width x 16m long for each legume, replicated three times, making a total of four blocks, and eight treatment units for each legume. Samples were collected at three different stages of growth of preanthesis, anthesis and post anthesis for biomass yield determination. A homogenous sub-sample was then oven dried for 72 hours at 60 0C. After drying the forage samples were milled through a 1mm screen and a 20g sample from each stage of growth and forage sample was subjected to proximate analysis, in-vitro digestibility and anti-nutrient evaluation. The in-vitro digestibility experiment was done with rumen liquor of cattle and sheep collected from an abbatoir. Tannins, total phenolics and sapponins were the only anti-nutrients evaluated as they are perceived to influence methanogenesis in ruminat animals. At 50percent flowering, forage legumes were harvested for hay making which was then used in an in-vivo trial to evaluate the nutritive value of forages. This trial was conducted using eighteen one-year-old goats of average weight 14.2±0.24 kg over a period of sixty days. The results showed that biomass yield, which increased with stage of growth, was significantly higher (P < 0.05) for Lablab than Cowpea (7.968t/ha vs 7.105t/ha). Cowpea exhibited higher moisture content (7.72percent), Ash (13.11percent), acid detergent fibre (ADF) (38.42percent) and crude protein (CP) (20.23percent) than Lablab which had 7.50percent, 11.45 percent, 36.17percent and 19percent, respectively. Lablab forage had significantly higher (P < 0.05) fat content (2.41percent), neutral detergent fibre (NDF) (49.27percent) and hemicellulose (13.07percent) than Cowpea which had 2.1percent; 46.91percent and 8.48percent, respectively. The stage of growth significantly (P < 0.05) affected the mineral content of both forages. Lablab showed significantly higher (P < 0.05) values (1.22percent 0.55percent, 2.67percent) than Cowpea (1.00percent, 0.45percent, 2.30percent) for calcium (Ca), phosphorus (P), and potassium (K) respectively. Lablab forage had significantly higher (P < 0.05) tannin (3.345mgCAE/gDM), phenolic (13.47mgGAE/gDM) and saponin (0.75percent) content compared to Cowpea with values of 1.494mgCAEg/DM; 9.402mgGAE/gDM and vs 1.55percent respectively. The tannin content significantly influenced in-vitro digestibility and enteric methane production. The forage type, harvesting stage and inoculum source significantly (P < 0.05) affected the in-vitro digestibility of the forages. The in-vitro dry matter (IVDM) and in-vitro organic matter (IVOM) digestibility was significantly higher (P < 0.05) for Lablab (41.13percent; 39.63percent) than Cowpea (39.86percent; 19.44percent), respectively. Both forages recorded higher dry matter (DM) digestibility coefficients at anthesis stage of growth 61.58percent, and 51.5 percent for Lablab and Cowpea respectively. The IVOM digestibility increased with stage of harvest (23.7percent to 25.57percent) for Cowpea while Lablab showed a decrease (44.6 percent to 43.9percent) when cattle inoculum was used. The IVDM digestibility was higher (P < 0.05) for Lablab (50.5percent) when cattle inoculum was used while Cowpea was observed to have higher digestibility co-efficiencies (38.54percent) when sheep inoculum was used. Cowpea had lower (P < 0.05) IVOM digestibility than Lablab (19.44percent) vs. 39.6percent). Lablab exhibited higher (P < 0.05) metabolisable energy (ME) values than Cowpea (6.21 vs. 2.70 MJ/kgDM). In the feeding study, the total DM intake by goats for Lablab was higher (P < 0.05) compared to Cowpea (688.1 vs. 607.8 g/head/ day). There was a positive (P < 0.05) correlation between dry matter intake and average daily gain (r= 0.8513, and r=0.5279) for Cowpea and Lablab respectively. Lablab and Cowpea promoted growth of Xhosa goats at a rate of 35.0g/d; and 38.3g/d, respectively which was not significantly different (P > 0.05). However, this growth was associated with a significantly higher (P < 0.05) food conversion ratio (FCR) of 19.16 vs 18.11 for Cowpea and Lablab, respectively. The apparent DM, non structural carbohydrate (NSC) nutrient digestibilities were significantly different (P < 0.05) for Lablab and Cowpea. Furthermore sex of animal influenced significantly (P < 0.05) the DM, ADF, NSC and NDF digestibilities. Lablab and Cowpea did not influence (P > 0.05) individual VFA molar concentrations in both sexes. However, animals fed Lablab had significantly higher butyrate concentrations (P < 0.05). Lablab and Cowpea forages significantly lowered (P > 0.05) enteric methane production in goats compared to a concentrate diet, and this was related to animal activity. Animals produced significantly more (P < 0.05) methane gas when ruminating than feeding or just standing. The average methane produced each year was lower but not significant (P > 0.05) for animals fed Cowpea than Lablab (4.602kg CH4, vs. 4.719kg CH4) when expressed per year. Male animals produced more gas than females with values of 17.40L/day; 12.46 g/kg DMI and 0.126g/day vs. 15.47L/day, 12.28 g/kg DMI, and 0.0109g/day, respectively. Forage legumes had no significant effects (P > 0.05) on serum total proteins (TP), globulin, urea, creatinine, alanine aminotransferase (ALT), alkaline phosphatase (ALP), and gamma- glutamyl transferase (GGT) concentrations, but serum concentration of albumin was influenced by the diets (P < 0.05). Sex of animals significantly influenced ALP concentration (P < 0.05) with male animals exhibiting higer ALP values than females. Measured over time, the plasma concentration of urea and ALP significantly increased (P < 0.05) while creatinine and cholesterol concentration decreased (P < 0.05). Although the overall serum concentration of urea, total proteins, globulin, creatinine, ALT, ALP and GGT remained unchanged among the treatments and between sexes; treatment by sex, treatment by day and sex by day interactions were significant (P < 0.05). The white blood cell (WBC), red blood cell (RBC), mean corpuscular haemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC), haemoglobin, haematocrite, platelet and mean platelet volume (MPV) levels were similar among treatments and between sexes (P > 0.05). The WBC decreased with time by 8.07 x10 9/l while mean crepuscular volume (MCV) increased by 11.83(fl) from day 15 to day 55. Treatment diets significantly (P < 0.05) influenced neutrophile (percent) content while sex influenced basophile (percent) content. There were no treatment or sex effects (P > 0.05) on lymphocytes (percent), monocytes (percent) and eosinophiles (percent). In the meat quality study, the dietary treatments and sex had a significant (P < 0.05) effect on dressing out percent (DPpercent), initial pH, redness (a*), yellowness (b*), Warner Blartze Shear force (WBS) , drip loss and cooking loss in the chevon from Xhosa goats. Compared with a commercial pellet diet, Lablab and Cowpea diets resulted in similar slaughter body weights (SBW), hot carcass weight (HCW) and cold dressed mass (CDM) of carcasses. However, female animals exhibited a significantly (P < 0.05) higher DPpercent than male animals (42.3percent vs. 38.9percent) for all treatments. The male animals exhibited significantly (P < 0.05) higher pH values one hour after slaughter than females (5.79 vs 5.67). Neverthreless the diet and gender did not significantly (P > 0.05) affect the ultimate pH (pHu). Meat samples from male animals had significantly (P < 0.05) higher redness values than females. Feeding cowpea significantly (P < 0.05) increased meat redness with the least drip loss of chevon. Female animals produced significantly (P < 0.05) tougher chevon compared to male animals; 4.16kg and 3.36kg for female and male, respectively. Lablab and Cowpea posses high CP levels and moderately lower NDF with high ADF values that influences dry matter intake (DMI), digestibility and growth of goats. These forage legumes meet animal requirements for fat, ADF, NDF, CP, Ca, Mn, zinc and Cu and their biomass yield is satisfactory.
- Full Text:
How phase feeding manipulation affects growth, performance, feed cost, carcass characteristics and the quality of meat from broilers
- Authors: Sokanyile, Sanda
- Date: 2017
- Subjects: Animal nutrition , Feeds , Broilers (Chickens)
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/5565 , vital:29334
- Description: The main objective of the study was to determine how phase feeding manipulation affects growth performance, feed cost, carcass characteristics and quality of meat from broiler chickens. A total of 180 day old un-sexed broiler chicks (Cobb 500) were separated into 3 dietary treatments of 60 birds per treatment and each treatment was replicated 3 times with 20 birds per replicate. The dietary treatments were as follows: T1 (starter 1-7 days; grower 8-21 days; finisher 22-35 days), T2 (starter 1-14 days; grower 15-21 days, finisher 22-35 days) and T3 (starter 1-14 days; grower 15-28 days; finisher 29-35 days). Feed intake and body weight gains were recorded weekly in kilograms. Average daily gain and feed conversion ratio were computed weekly. At day 35, the chickens were slaughtered and 12 representative breast muscles from each treatment were used for the determination of muscle pH (pH1, pH24 and pH48) and colour coordinates (Lightness – L*, redness – a* and yellowness – b*) in triplicate at 1 hour, 24 hours and 48 hours post-mortem.Feed intake (FI), feed conversion ratio (FCR), average daily gain (ADG) and average body weight at slaughter (ABW) were not significant (P>0.05) amongst the treatments. The most cost efficient feed (which accumulated the cheapest cost feed per kg were) T1 (R11.32) and T3 (R11.32) although there was a slight difference in the gross profit of these treatments T1 (R45.71) and T3 (R44.48). Carcass characteristics were the same (P > 0.05) across the treatments except for the wing, thigh, drumstick and the breast (P < 0.05). T2 had the highest wing weight (166.63±8.60), T1 (113.03±8.60) and the lowest in T3 (74.46±8.60). The thigh weight were greater (P<0.05) in T2 (185.69±4.34); T1 (185.54±4.34) compared to T3 (166.97±4.34) which was lower. Treatments 1 (204.17±6.57), T2 (197±6.57) had heavier (P<0.05) breast weights than T3 (186.06±6.57). Dietary treatments had no significant effect (P > 0.05) on pH of the breast meat. No differences were observed in colour during the 1 hour period after slaughter. At 24 hours after slaughter, the L* values were different (P < 0.05) in T1 and T2 (44.3 ± 0.37; 43.7 ± 0.37), respectively, T1 and T3 were the same. The a*-values were different (P < 0.05) at 48 hours after slaughter for T1 and T3 (4.5 ± 0.27; 3.4 ± 0.27), T2 was the same as T1. It was therefore, concluded that since manipulation of starter phase did not have adverse effects on growth performance, gross profit, slight statistical difference in meat quality attributes and carcass characteristics. Therefore, the manipulation of starter diet has the potential to be used in broiler production.
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- Authors: Sokanyile, Sanda
- Date: 2017
- Subjects: Animal nutrition , Feeds , Broilers (Chickens)
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/5565 , vital:29334
- Description: The main objective of the study was to determine how phase feeding manipulation affects growth performance, feed cost, carcass characteristics and quality of meat from broiler chickens. A total of 180 day old un-sexed broiler chicks (Cobb 500) were separated into 3 dietary treatments of 60 birds per treatment and each treatment was replicated 3 times with 20 birds per replicate. The dietary treatments were as follows: T1 (starter 1-7 days; grower 8-21 days; finisher 22-35 days), T2 (starter 1-14 days; grower 15-21 days, finisher 22-35 days) and T3 (starter 1-14 days; grower 15-28 days; finisher 29-35 days). Feed intake and body weight gains were recorded weekly in kilograms. Average daily gain and feed conversion ratio were computed weekly. At day 35, the chickens were slaughtered and 12 representative breast muscles from each treatment were used for the determination of muscle pH (pH1, pH24 and pH48) and colour coordinates (Lightness – L*, redness – a* and yellowness – b*) in triplicate at 1 hour, 24 hours and 48 hours post-mortem.Feed intake (FI), feed conversion ratio (FCR), average daily gain (ADG) and average body weight at slaughter (ABW) were not significant (P>0.05) amongst the treatments. The most cost efficient feed (which accumulated the cheapest cost feed per kg were) T1 (R11.32) and T3 (R11.32) although there was a slight difference in the gross profit of these treatments T1 (R45.71) and T3 (R44.48). Carcass characteristics were the same (P > 0.05) across the treatments except for the wing, thigh, drumstick and the breast (P < 0.05). T2 had the highest wing weight (166.63±8.60), T1 (113.03±8.60) and the lowest in T3 (74.46±8.60). The thigh weight were greater (P<0.05) in T2 (185.69±4.34); T1 (185.54±4.34) compared to T3 (166.97±4.34) which was lower. Treatments 1 (204.17±6.57), T2 (197±6.57) had heavier (P<0.05) breast weights than T3 (186.06±6.57). Dietary treatments had no significant effect (P > 0.05) on pH of the breast meat. No differences were observed in colour during the 1 hour period after slaughter. At 24 hours after slaughter, the L* values were different (P < 0.05) in T1 and T2 (44.3 ± 0.37; 43.7 ± 0.37), respectively, T1 and T3 were the same. The a*-values were different (P < 0.05) at 48 hours after slaughter for T1 and T3 (4.5 ± 0.27; 3.4 ± 0.27), T2 was the same as T1. It was therefore, concluded that since manipulation of starter phase did not have adverse effects on growth performance, gross profit, slight statistical difference in meat quality attributes and carcass characteristics. Therefore, the manipulation of starter diet has the potential to be used in broiler production.
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The effect of quantitative feed restriction on growth performance, carcass characteristics and selected meat quality parameters in broiler chickens
- Authors: Velele, Siphelo
- Date: 2017
- Subjects: Broilers (Chickens) Poultry -- Breeding Meat -- Quality
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/8888 , vital:33907
- Description: The study investigated the effects of quantitative feed restriction on growth performance, carcass characteristics, internal organs, breast muscle pH and meat colour in broiler chickens. A total of 90, un-sexed, day-old broiler chicks were procured from an accredited supplier and were used for the current trial. For the first 14 days, all birds were brooded in one house and subjected to a uniform day-to-day management. Water was supplied throughout the trial, whereas, feed was only supplied ad libitum between days 1-14 and 29-35 days in feed restricted birds. Feed phases included starter (1-14 days), grower (15-28) and finisher (29-35), respectively. On day 15, birds were randomly allocated to three treatments; each treatment was replicated three times with 10 birds per replicate. The first treatment (T1) group, which acted as control group, was ad libitum feeding for the whole trial duration (1-35 days). For treatments 2 (T2) and 3(T3), 85 percent and 70 percent of the Cobb 500 broiler feed intake standards were applied for a period of 14 days (days 15-28), respectively. Average daily feed intake (ADFI) was determined daily and then average body weight (ABW) was determined weekly. Feed conversion ratio and average daily weight gain was thus computed for each experimental unit (replicate). On day 35, broiler birds were electrically stunned and slaughtered by a sharp knife and then hung for complete bleeding. Data collected included slaughter, carcass, breast, thigh, wing, drumstick, feet, head, spleen, heart, gizzard, intestines (small & large) and liver weights. Breast muscle was further used for the determination of colour (L*, b* and a*), initial (pHi) and ultimate breast pH (pHu) measurements. Although, quantitative feed restriction (T2 & T3) significantly affected growth performance in weeks 3 and 4, birds submitted to T2 performed similar (P>0.05) to control in the final stage, whilst birds in T3 was the poorest (P<0.05). Furthermore, birds subjected to T2 showed no differences of edible carcass portions to the control, however, T3 group showed lower (P<0.05) slaughter and wing weights. Breast ultimate pH values of birds under T2 were similar to control, but birds in T3 had significantly higher (P<0.05) values. Birds under T2 had less (P<0.05) reddish breast meat colour than control at 45 minutes. At 24 hours post-mortem, birds in T3 had lower (P<0.05) lightness values and birds submitted to T2 had higher (P<0.05) yellowness values. Liver weight was significantly lower in birds under T3 and higher (P<0.05) gizzard weights were found in birds subjected to T2. Birds under T2 performed similar to control and were able to compensate for the weight loss when high plane of feed was re-introduced. It can be concluded from the results that restricting 15 percent of feed from Cobb 500 standard feed intake had moderately affected broiler performance. Moreover, restricting 15percent of feed significantly reduced feed intake in broiler chicken.
- Full Text:
- Authors: Velele, Siphelo
- Date: 2017
- Subjects: Broilers (Chickens) Poultry -- Breeding Meat -- Quality
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/8888 , vital:33907
- Description: The study investigated the effects of quantitative feed restriction on growth performance, carcass characteristics, internal organs, breast muscle pH and meat colour in broiler chickens. A total of 90, un-sexed, day-old broiler chicks were procured from an accredited supplier and were used for the current trial. For the first 14 days, all birds were brooded in one house and subjected to a uniform day-to-day management. Water was supplied throughout the trial, whereas, feed was only supplied ad libitum between days 1-14 and 29-35 days in feed restricted birds. Feed phases included starter (1-14 days), grower (15-28) and finisher (29-35), respectively. On day 15, birds were randomly allocated to three treatments; each treatment was replicated three times with 10 birds per replicate. The first treatment (T1) group, which acted as control group, was ad libitum feeding for the whole trial duration (1-35 days). For treatments 2 (T2) and 3(T3), 85 percent and 70 percent of the Cobb 500 broiler feed intake standards were applied for a period of 14 days (days 15-28), respectively. Average daily feed intake (ADFI) was determined daily and then average body weight (ABW) was determined weekly. Feed conversion ratio and average daily weight gain was thus computed for each experimental unit (replicate). On day 35, broiler birds were electrically stunned and slaughtered by a sharp knife and then hung for complete bleeding. Data collected included slaughter, carcass, breast, thigh, wing, drumstick, feet, head, spleen, heart, gizzard, intestines (small & large) and liver weights. Breast muscle was further used for the determination of colour (L*, b* and a*), initial (pHi) and ultimate breast pH (pHu) measurements. Although, quantitative feed restriction (T2 & T3) significantly affected growth performance in weeks 3 and 4, birds submitted to T2 performed similar (P>0.05) to control in the final stage, whilst birds in T3 was the poorest (P<0.05). Furthermore, birds subjected to T2 showed no differences of edible carcass portions to the control, however, T3 group showed lower (P<0.05) slaughter and wing weights. Breast ultimate pH values of birds under T2 were similar to control, but birds in T3 had significantly higher (P<0.05) values. Birds under T2 had less (P<0.05) reddish breast meat colour than control at 45 minutes. At 24 hours post-mortem, birds in T3 had lower (P<0.05) lightness values and birds submitted to T2 had higher (P<0.05) yellowness values. Liver weight was significantly lower in birds under T3 and higher (P<0.05) gizzard weights were found in birds subjected to T2. Birds under T2 performed similar to control and were able to compensate for the weight loss when high plane of feed was re-introduced. It can be concluded from the results that restricting 15 percent of feed from Cobb 500 standard feed intake had moderately affected broiler performance. Moreover, restricting 15percent of feed significantly reduced feed intake in broiler chicken.
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Effect of Moringa oleifera leaf meal on growth performance, faecal ammonia emissions, carcass characteristics and the quality of meat from broiler chickens
- Authors: Mxunyelwa, Avela,
- Date: 2016
- Subjects: Moringa oleifera Meat -- Quality Broilers (Chickens)
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/15034 , vital:40154
- Description: The objective of the study was to determine growth performance, faecal ammonia gas emission, carcass characteristics and physico-chemical meat traits from broilers fed one of the four dietary treatments, containing 0percent, 1percent, 3percent or 5percent Moringa oleifera leaf meal (MOLM) (T1, T2, T3, and T4, respectively). Dietary treatments were formulated to be isonitrogenous and isocaloric for a starter (0-14 days), grower (15-21days) and finisher (22-35 days). A total of 160 day old Cobb 500 broiler chickens, initially weighing an average of 45g were randomly allocated to each of the dietary treatments, each with four replicates, in 16 pens. Feed and water were offered ad libitum. Feed intakes (FI), average daily gain (ADG), body weight gain (BWG) and feed conversion efficiency (FCE) were measured weekly. At the end of the feeding trial, the feed was withdrawn from the birds for 12 hours with water available ad libitum and the birds were slaughtered using standard abattoir procedures. The breast muscle of each carcass was sampled for meat quality analysis. Organ and carcass weights differences were measured. Birds fed with T1 had the highest FI. Birds fed with T4 had the best FCE at day 14, 21 and 35. At day 7 and 35, there were no significant differences in FCE in all the treatments. At 0 to 35 days, faecal ammonia emission decreased (P<0.05) as MOLM concentration and age of birds increased. Moringa fed broilers had the highest body weights in all the periods despite the incorporation level in the diet. The results showed that inclusion of MOLM had a significant effect (P<0.05) on the heart, neck, and head weights but had no significant effect on liver, gizzard, intestine and feet. Broilers fed with T1 had heavier heart, neck and head weights compared to MOLM fed broilers. In terms of carcass traits, MOLM had a significant effect (P<0.05) on wings and thighs but had no significant effect (P>0.05) on breast and drumsticks. Moringa oleifera leaf meal had a significant effect on carcass weights, control diet had heavier weights (1185g ± 28.96) compared to chickens fed with MOLM diets (1121g±28.96, 1070g ± 28.96, 1030g± 28.96). Broilers fed with MOLM had higher values of lightness (L*) and (b*) values (P<0.05) at 45 minutes and 24 hours and no difference was observed in redness (a*) at 45 minutes and 24 hours. The L* values dropped at 24 hours for all the treatments and MOLM fed broilers had the lowest values (46.59 ± 0.76,46 ± 0.76,49 ± 0.76) compared to the control diet (50 ± 0.76). The pH levels were comparable after 24 hours; T2 had the highest pH values (5.95 ± 0.024) compared to other treatments (5.90 ± 0.024, 5.75 ± 0.024, 5.73 ± 0.024). The results of this study demonstrated that even though the FI was reduced in MOLM fed broilers, they efficiently consumed and used nutrients, as a result MOLM fed birds had higher weights and low ammonia concentration throughout the experiment, which may improve the house environment without affecting the quality of meat.
- Full Text:
- Authors: Mxunyelwa, Avela,
- Date: 2016
- Subjects: Moringa oleifera Meat -- Quality Broilers (Chickens)
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/15034 , vital:40154
- Description: The objective of the study was to determine growth performance, faecal ammonia gas emission, carcass characteristics and physico-chemical meat traits from broilers fed one of the four dietary treatments, containing 0percent, 1percent, 3percent or 5percent Moringa oleifera leaf meal (MOLM) (T1, T2, T3, and T4, respectively). Dietary treatments were formulated to be isonitrogenous and isocaloric for a starter (0-14 days), grower (15-21days) and finisher (22-35 days). A total of 160 day old Cobb 500 broiler chickens, initially weighing an average of 45g were randomly allocated to each of the dietary treatments, each with four replicates, in 16 pens. Feed and water were offered ad libitum. Feed intakes (FI), average daily gain (ADG), body weight gain (BWG) and feed conversion efficiency (FCE) were measured weekly. At the end of the feeding trial, the feed was withdrawn from the birds for 12 hours with water available ad libitum and the birds were slaughtered using standard abattoir procedures. The breast muscle of each carcass was sampled for meat quality analysis. Organ and carcass weights differences were measured. Birds fed with T1 had the highest FI. Birds fed with T4 had the best FCE at day 14, 21 and 35. At day 7 and 35, there were no significant differences in FCE in all the treatments. At 0 to 35 days, faecal ammonia emission decreased (P<0.05) as MOLM concentration and age of birds increased. Moringa fed broilers had the highest body weights in all the periods despite the incorporation level in the diet. The results showed that inclusion of MOLM had a significant effect (P<0.05) on the heart, neck, and head weights but had no significant effect on liver, gizzard, intestine and feet. Broilers fed with T1 had heavier heart, neck and head weights compared to MOLM fed broilers. In terms of carcass traits, MOLM had a significant effect (P<0.05) on wings and thighs but had no significant effect (P>0.05) on breast and drumsticks. Moringa oleifera leaf meal had a significant effect on carcass weights, control diet had heavier weights (1185g ± 28.96) compared to chickens fed with MOLM diets (1121g±28.96, 1070g ± 28.96, 1030g± 28.96). Broilers fed with MOLM had higher values of lightness (L*) and (b*) values (P<0.05) at 45 minutes and 24 hours and no difference was observed in redness (a*) at 45 minutes and 24 hours. The L* values dropped at 24 hours for all the treatments and MOLM fed broilers had the lowest values (46.59 ± 0.76,46 ± 0.76,49 ± 0.76) compared to the control diet (50 ± 0.76). The pH levels were comparable after 24 hours; T2 had the highest pH values (5.95 ± 0.024) compared to other treatments (5.90 ± 0.024, 5.75 ± 0.024, 5.73 ± 0.024). The results of this study demonstrated that even though the FI was reduced in MOLM fed broilers, they efficiently consumed and used nutrients, as a result MOLM fed birds had higher weights and low ammonia concentration throughout the experiment, which may improve the house environment without affecting the quality of meat.
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Glycolytic potential and meat quality from dorper and merino sheep slaughtered at a commercial abattoir
- Authors: Stempa, Thuthuzelwa
- Date: 2015
- Subjects: Slaughtering and slaughter-houses Meat -- Quality
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/15310 , vital:40361
- Description: The objective of the study was to determine glycolytic potential and meat quality from Dorper and Merino sheep of both sexes slaughtered at a commercial abattoir. Dorper (n=52) and Merino (n= 48) breeds aged eight years, consisting of 50 intact rams and 50 non-pregnant ewes were used in the study. The sheep used in the study were reared, transported and lairised under identical conditions. Blood samples were collected at exsanguination for the measurement of glucose, lactate and cortisol levels. Samples were also collected from the Muscularis longmissius thoracis et lumborum (LTL) for the measurement glycogen, lactate levels, pH decline and colour. Correlations amongst blood stress indicators, muscle metabolites and meat quality attributes were also determined. Sex and breed had no effect on muscle glycolytic potential, glycogen and lactate levels from Dorper and Merino sheep of both sexes at the abattoir. Although sex and breed had an effect on pre-slaughter stress indicators (lactate and cortisol) collected at exsanguination. Ewes had higher levels of blood lactate (7.4 3 ± 0.49 mmol/L) and cortisol (293.92 ± 14.32 nmol/L) than the rams which had (5.19 ± 0.49 mmol/L) and (179.50 ± 14.32 nmol/L) lactate and cortisol levels, respectively. Furthermore, higher levels of lactate were observed in Dorper (7.54 ± 0.42 mmol/L) compared to the Merino sheep (4.97 ± 0.49 mmol/L). Meat pH decline and colour were also significantly affected by sex and breed. Ewes had higher levels of at pH45 minutes post slaughter (7.05 ± 0.04), pH3 hours (6.45 ± 0.04) , pH24 hours (6.00 ± 0.03), a* (14.31 ± 0.33) , b* (8.84 ± 0.29), H* (31.47 ± 0.73) and C* (16.75 ± 0.24) compared to the rams which had pH45 minutes (6.44 ± 0.04), pH3 hours (6.12 ± 0.04), pH24 hours (5.88 ± 0.03), a* (12.25 ± 0.33), b* (7.00 ± 0.29), H* (29.36 ± 0.73) and C* (14.15 ± 0.42) values. Moreover, Merino sheep had higher levels of L* (38.17 ± 0.48) and H* (31.59 ± 0.74) compared to the Dorper which had (36.39 ± 0.46) and (29.33 ± 0.71) L* and H* values, respectively. Blood cortisol was also positively correlated (P < 0.05) to glucose (r = 0.27), lactate (r = 0.37) but was negatively correlated (P < 0.001) to meat lightness (r = -0.44). Furthermore, blood cortisol was positively correlated (P < 0.001) to pH45 (r = 0.34), pH24 (r =0.22), meat yellowness (r = 0.24) and chroma (r = 0.37), but was negatively correlated to meat lightness (r = -0.47). Glycolytic potential was positively correlated (P < 0.001) to muscle glycogen levels (r = 0.66) and muscle lactate (r = 0.71).
- Full Text:
- Authors: Stempa, Thuthuzelwa
- Date: 2015
- Subjects: Slaughtering and slaughter-houses Meat -- Quality
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/15310 , vital:40361
- Description: The objective of the study was to determine glycolytic potential and meat quality from Dorper and Merino sheep of both sexes slaughtered at a commercial abattoir. Dorper (n=52) and Merino (n= 48) breeds aged eight years, consisting of 50 intact rams and 50 non-pregnant ewes were used in the study. The sheep used in the study were reared, transported and lairised under identical conditions. Blood samples were collected at exsanguination for the measurement of glucose, lactate and cortisol levels. Samples were also collected from the Muscularis longmissius thoracis et lumborum (LTL) for the measurement glycogen, lactate levels, pH decline and colour. Correlations amongst blood stress indicators, muscle metabolites and meat quality attributes were also determined. Sex and breed had no effect on muscle glycolytic potential, glycogen and lactate levels from Dorper and Merino sheep of both sexes at the abattoir. Although sex and breed had an effect on pre-slaughter stress indicators (lactate and cortisol) collected at exsanguination. Ewes had higher levels of blood lactate (7.4 3 ± 0.49 mmol/L) and cortisol (293.92 ± 14.32 nmol/L) than the rams which had (5.19 ± 0.49 mmol/L) and (179.50 ± 14.32 nmol/L) lactate and cortisol levels, respectively. Furthermore, higher levels of lactate were observed in Dorper (7.54 ± 0.42 mmol/L) compared to the Merino sheep (4.97 ± 0.49 mmol/L). Meat pH decline and colour were also significantly affected by sex and breed. Ewes had higher levels of at pH45 minutes post slaughter (7.05 ± 0.04), pH3 hours (6.45 ± 0.04) , pH24 hours (6.00 ± 0.03), a* (14.31 ± 0.33) , b* (8.84 ± 0.29), H* (31.47 ± 0.73) and C* (16.75 ± 0.24) compared to the rams which had pH45 minutes (6.44 ± 0.04), pH3 hours (6.12 ± 0.04), pH24 hours (5.88 ± 0.03), a* (12.25 ± 0.33), b* (7.00 ± 0.29), H* (29.36 ± 0.73) and C* (14.15 ± 0.42) values. Moreover, Merino sheep had higher levels of L* (38.17 ± 0.48) and H* (31.59 ± 0.74) compared to the Dorper which had (36.39 ± 0.46) and (29.33 ± 0.71) L* and H* values, respectively. Blood cortisol was also positively correlated (P < 0.05) to glucose (r = 0.27), lactate (r = 0.37) but was negatively correlated (P < 0.001) to meat lightness (r = -0.44). Furthermore, blood cortisol was positively correlated (P < 0.001) to pH45 (r = 0.34), pH24 (r =0.22), meat yellowness (r = 0.24) and chroma (r = 0.37), but was negatively correlated to meat lightness (r = -0.47). Glycolytic potential was positively correlated (P < 0.001) to muscle glycogen levels (r = 0.66) and muscle lactate (r = 0.71).
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The effect of grain and soya bean-based diets on chicken production, some egg quality traits, and the potential for allergen carryover to eggs and meat
- Authors: Dokora, Adia-En-Michelle
- Date: 2015
- Subjects: Chickens -- Nutrition Chickens --Feeding and feeds Meat -- Quality
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/2946 , vital:28192
- Description: This study determined the effect of maize-, whole wheat-, soya bean-based-diets on the growth performance, egg quality and dressing percentage of chickens, as well as the potential for soy and gluten allergen carryover to eggs and meat from chickens. Twenty 36-week-old Lorham White (LW) hens were divided into two groups and kept in individual cages until they reached 39-weeks of age, with water and feed supplied ad libitum. Ten LW hens were fed a maize/soya bean-based diet (T1) and the other ten birds were fed a maize/soya bean-based diet with a 15 percent whole wheat inclusion (T2) for a period of four weeks, with weekly individual hen weights and group feed consumption figures being recorded. After a two-week diet adaptation period, six eggs per treatment group were collected every second day from the LW hens’ to measure egg quality traits. Every third day, over a 29-day period, six eggs per treatment were collected and analysed for the presence of soy, gluten and gluten-derived peptides using allergen specific enzyme linked immunosorbent assays (ELISA) kits. For the broiler trial, a total of 160 Ross 308 mixed sex day old chicks were used in a completely randomized design. Two dietary treatments were assigned to eight cages (replicate) per treatment, with ten birds per cage. The Ross 308 broiler feeding programme consisted of three phases, starter (day 1 to day 10), grower (day 11 to day 20) and finisher (day 21 to day 28). At the beginning of the trial all chicks were fed T1 which contained a maize/soya bean-based diet for a period of 10 days and then eight cages with 10 birds per cage were randomly selected and fed the T2 diet, which contained a maize/soya bean-based diet with a 15 percent whole wheat inclusion, until they reached 28 days of age. Weekly live weights, feed intake, average daily gain and the average daily feed intake were recorded and calculated. At the end of the grower (day 21) and finisher phase (day 28), one broiler bird was selected per cage to have eight (8) birds per treatment and 16 birds per phase that were slaughtered and breast meat samples were analysed in duplicate for the presence of soy and gluten allergens using ELISA kits. The dressing percentage of hot carcasses was also determined at the end of the finisher phase on eight birds per treatment. For LW hens, live weight (LW) at 39 weeks of age, the average daily feed intake (ADFI) and weekly feed intakes (FI) were significantly different (P < 0.05) between treatments with birds on T2 recording higher gains. As birds age in weeks increased, significant differences (P < 0.05) in live weight values were recorded. Egg quality traits measured (shell weight, yolk weight, albumen weight, albumen height and Haugh units) were significantly different (P < 0.05) between treatments, with eggs from hens receiving T1 recording higher mean values. As hens got older, the colour of egg yolk improved, producing eggs with higher b* (yellowness) values and lower L* (lightness) and a* (redness) mean values. The Haugh unit, significantly improved (P < 0.05) as the hens got older, signifying better quality of eggs. For the broiler birds, growth traits measured indicated that there were no significant differences (P > 0.05) in live weight (LW), average daily feed intake (ADFI), weekly feed intake (FI), feed conversion ratio (FCR), cumulative feed intake (CFI) and cumulative gain (CG) between treatments. No significant differences (P > 0.05) between dressing percentage of broilers from the two treatments were observed. As birds grew, significant differences (P < 0.05) in LW, FI, AFI, FCR, and CFI, over a four-week period were recorded. Results of the allergen analyses indicated that soy and gluten allergens were not carried over from feed into eggs and meat of chickens fed a maize/soya bean-based diet and a maize/soya bean-based with a 15 percent whole wheat inclusion, respectively. In conclusion, a maize/soya bean-based diet and maize/soya bean-based diet with a 15 percent whole wheat inclusion that contained soy and gluten allergens, are feed sources that promote and stimulate 36-week-old LW hen and Ross 308 chicken growth.
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- Authors: Dokora, Adia-En-Michelle
- Date: 2015
- Subjects: Chickens -- Nutrition Chickens --Feeding and feeds Meat -- Quality
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/2946 , vital:28192
- Description: This study determined the effect of maize-, whole wheat-, soya bean-based-diets on the growth performance, egg quality and dressing percentage of chickens, as well as the potential for soy and gluten allergen carryover to eggs and meat from chickens. Twenty 36-week-old Lorham White (LW) hens were divided into two groups and kept in individual cages until they reached 39-weeks of age, with water and feed supplied ad libitum. Ten LW hens were fed a maize/soya bean-based diet (T1) and the other ten birds were fed a maize/soya bean-based diet with a 15 percent whole wheat inclusion (T2) for a period of four weeks, with weekly individual hen weights and group feed consumption figures being recorded. After a two-week diet adaptation period, six eggs per treatment group were collected every second day from the LW hens’ to measure egg quality traits. Every third day, over a 29-day period, six eggs per treatment were collected and analysed for the presence of soy, gluten and gluten-derived peptides using allergen specific enzyme linked immunosorbent assays (ELISA) kits. For the broiler trial, a total of 160 Ross 308 mixed sex day old chicks were used in a completely randomized design. Two dietary treatments were assigned to eight cages (replicate) per treatment, with ten birds per cage. The Ross 308 broiler feeding programme consisted of three phases, starter (day 1 to day 10), grower (day 11 to day 20) and finisher (day 21 to day 28). At the beginning of the trial all chicks were fed T1 which contained a maize/soya bean-based diet for a period of 10 days and then eight cages with 10 birds per cage were randomly selected and fed the T2 diet, which contained a maize/soya bean-based diet with a 15 percent whole wheat inclusion, until they reached 28 days of age. Weekly live weights, feed intake, average daily gain and the average daily feed intake were recorded and calculated. At the end of the grower (day 21) and finisher phase (day 28), one broiler bird was selected per cage to have eight (8) birds per treatment and 16 birds per phase that were slaughtered and breast meat samples were analysed in duplicate for the presence of soy and gluten allergens using ELISA kits. The dressing percentage of hot carcasses was also determined at the end of the finisher phase on eight birds per treatment. For LW hens, live weight (LW) at 39 weeks of age, the average daily feed intake (ADFI) and weekly feed intakes (FI) were significantly different (P < 0.05) between treatments with birds on T2 recording higher gains. As birds age in weeks increased, significant differences (P < 0.05) in live weight values were recorded. Egg quality traits measured (shell weight, yolk weight, albumen weight, albumen height and Haugh units) were significantly different (P < 0.05) between treatments, with eggs from hens receiving T1 recording higher mean values. As hens got older, the colour of egg yolk improved, producing eggs with higher b* (yellowness) values and lower L* (lightness) and a* (redness) mean values. The Haugh unit, significantly improved (P < 0.05) as the hens got older, signifying better quality of eggs. For the broiler birds, growth traits measured indicated that there were no significant differences (P > 0.05) in live weight (LW), average daily feed intake (ADFI), weekly feed intake (FI), feed conversion ratio (FCR), cumulative feed intake (CFI) and cumulative gain (CG) between treatments. No significant differences (P > 0.05) between dressing percentage of broilers from the two treatments were observed. As birds grew, significant differences (P < 0.05) in LW, FI, AFI, FCR, and CFI, over a four-week period were recorded. Results of the allergen analyses indicated that soy and gluten allergens were not carried over from feed into eggs and meat of chickens fed a maize/soya bean-based diet and a maize/soya bean-based with a 15 percent whole wheat inclusion, respectively. In conclusion, a maize/soya bean-based diet and maize/soya bean-based diet with a 15 percent whole wheat inclusion that contained soy and gluten allergens, are feed sources that promote and stimulate 36-week-old LW hen and Ross 308 chicken growth.
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