A xylem retrieval pathway in rice leaf blades – evidence of a role for endocytosis?
- Botha, Christiaan E J, Aoki, N, Scofield, G N, Liu, Lin, Furbank, R T, White, R G
- Authors: Botha, Christiaan E J , Aoki, N , Scofield, G N , Liu, Lin , Furbank, R T , White, R G
- Date: 2008
- Language: English
- Type: Article
- Identifier: vital:6505 , http://hdl.handle.net/10962/d1005930 , http://dx.doi.org/10.1093/jxb/ern150
- Description: The structure and transport properties of pit membranes at the interface between the metaxylem and xylem parenchyma cells and the possible role of these pit membranes in solute transfer to the phloem was investigated. Electron microscopy revealed a fibrillar, almost tubular matrix within the pit membrane structure between the xylem vessels and xylem parenchyma of leaf blade bundles in rice (Oryza sativa). These pits are involved primarily with regulating water flux to the surrounding xylem parenchyma cells. Vascular parenchyma cells contain large mitochondrial populations, numerous dictyosomes, endomembrane complexes and vesicles in close proximity to the pit membrane. Taken collectively this suggests that endocytosis may occur at this interface. A weak solution of 5,6-carboxyfluorescein diacetate (5,6-CFDA) was applied to cut ends of leaves and after a minimum of 45 minutes, the distribution of the fluorescent cleavage product, 5,6-carboxyfluorescein (5,6-CF) was observed using confocal microscopy. Cleavage of 5,6-CFDA occurred within the xylem parenchyma cells and the non-polar 5,6-CF was then symplasmically transported to other parenchyma elements and ultimately, via numerous pore plasmodesmata, to adjacent thick-walled sieve tubes. Application of Lucifer Yellow, and, separately, Texas Red labelled dextran (10 kDa) to the transpiration stream, confirmed that these membrane-impermeant probes could only have been offloaded from the xylem via the xylem vessel-xylem parenchyma pit membranes, suggesting endocytotic transmembrane transfer of these membrane-impermeant fluorophores. Accumulation within the thick-walled sieve tubes, but not in thin-walled sieve tubes, confirms the presence of a symplasmic phloem loading pathway, via pore plasmodesmata between xylem parenchyma and thick-walled sieve tubes, but not thin-walled sieve tubes.
- Full Text:
- Date Issued: 2008
- Authors: Botha, Christiaan E J , Aoki, N , Scofield, G N , Liu, Lin , Furbank, R T , White, R G
- Date: 2008
- Language: English
- Type: Article
- Identifier: vital:6505 , http://hdl.handle.net/10962/d1005930 , http://dx.doi.org/10.1093/jxb/ern150
- Description: The structure and transport properties of pit membranes at the interface between the metaxylem and xylem parenchyma cells and the possible role of these pit membranes in solute transfer to the phloem was investigated. Electron microscopy revealed a fibrillar, almost tubular matrix within the pit membrane structure between the xylem vessels and xylem parenchyma of leaf blade bundles in rice (Oryza sativa). These pits are involved primarily with regulating water flux to the surrounding xylem parenchyma cells. Vascular parenchyma cells contain large mitochondrial populations, numerous dictyosomes, endomembrane complexes and vesicles in close proximity to the pit membrane. Taken collectively this suggests that endocytosis may occur at this interface. A weak solution of 5,6-carboxyfluorescein diacetate (5,6-CFDA) was applied to cut ends of leaves and after a minimum of 45 minutes, the distribution of the fluorescent cleavage product, 5,6-carboxyfluorescein (5,6-CF) was observed using confocal microscopy. Cleavage of 5,6-CFDA occurred within the xylem parenchyma cells and the non-polar 5,6-CF was then symplasmically transported to other parenchyma elements and ultimately, via numerous pore plasmodesmata, to adjacent thick-walled sieve tubes. Application of Lucifer Yellow, and, separately, Texas Red labelled dextran (10 kDa) to the transpiration stream, confirmed that these membrane-impermeant probes could only have been offloaded from the xylem via the xylem vessel-xylem parenchyma pit membranes, suggesting endocytotic transmembrane transfer of these membrane-impermeant fluorophores. Accumulation within the thick-walled sieve tubes, but not in thin-walled sieve tubes, confirms the presence of a symplasmic phloem loading pathway, via pore plasmodesmata between xylem parenchyma and thick-walled sieve tubes, but not thin-walled sieve tubes.
- Full Text:
- Date Issued: 2008
Xylem as well as phloem sustains severe damage due to feeding by the Russian wheat aphid
- Saheed, S A, Liu, Lin, Jonsson, Lisbeth M V, Botha, Christiaan E J
- Authors: Saheed, S A , Liu, Lin , Jonsson, Lisbeth M V , Botha, Christiaan E J
- Date: 2007
- Language: English
- Type: Article
- Identifier: vital:6541 , http://hdl.handle.net/10962/d1005983
- Description: Investigation of comparative effects of feeding damage by the Russian wheat aphid (RWA, biotype SA1, Diuraphis noxia Mordvilko) on leaf blades of susceptible and resistant wheat cultivars (Triticum aestivum L. var Betta and Betta-Dn1 respectively) were carried out to establish the level of ultrastructural damage caused by this aphid and the possible limitation of damage induced which could be ascribed to the resistance gene Dn1 over the susceptible cultivar. Ultrastructurally, Betta-Dn1 sustained less damage to the vascular tissue as well as to the mesophyll during the experimental period. Both inter- and intracellular probes resulted in considerable saliva deposition as the aphids probed for suitable feeding sites. Salivary tracks were observed between and within mesophyll, bundle sheath cells as well as the vascular tissue, including the xylem. Disruption of organelles and cytoplasm resulted from cell probing and sheath deposition. Cell and organelle damage was more evident in the non-resistant Betta cultivar. The aphids probed for and fed from thin-walled sieve tubes preferentially. Few thick-walled sieve tubes showed evidence of either aphid probing or feeding-related damage. Saliva was deposited when the aphids probed inter- and intracellularly for feeding sites. The aphids appeared preferentially to probe for and feed from thin-walled sieve tubes, as few thick-walled sieve tubes showed evidence of damage. Vessels, apparently probed for water, contained watery saliva that encased the secondary walls and sealed pit membranes between probed vessels and xylem parenchyma. The xylem probed by the RWA was rendered non-functional, probably contributing to symptoms of leaf roll, chlorosis and necrosis, which were observed within two weeks of infestation in the susceptible Betta cultivar. This damage was limited in the resistant Betta-Dn1 cultivar during the same time frame.
- Full Text:
- Date Issued: 2007
- Authors: Saheed, S A , Liu, Lin , Jonsson, Lisbeth M V , Botha, Christiaan E J
- Date: 2007
- Language: English
- Type: Article
- Identifier: vital:6541 , http://hdl.handle.net/10962/d1005983
- Description: Investigation of comparative effects of feeding damage by the Russian wheat aphid (RWA, biotype SA1, Diuraphis noxia Mordvilko) on leaf blades of susceptible and resistant wheat cultivars (Triticum aestivum L. var Betta and Betta-Dn1 respectively) were carried out to establish the level of ultrastructural damage caused by this aphid and the possible limitation of damage induced which could be ascribed to the resistance gene Dn1 over the susceptible cultivar. Ultrastructurally, Betta-Dn1 sustained less damage to the vascular tissue as well as to the mesophyll during the experimental period. Both inter- and intracellular probes resulted in considerable saliva deposition as the aphids probed for suitable feeding sites. Salivary tracks were observed between and within mesophyll, bundle sheath cells as well as the vascular tissue, including the xylem. Disruption of organelles and cytoplasm resulted from cell probing and sheath deposition. Cell and organelle damage was more evident in the non-resistant Betta cultivar. The aphids probed for and fed from thin-walled sieve tubes preferentially. Few thick-walled sieve tubes showed evidence of either aphid probing or feeding-related damage. Saliva was deposited when the aphids probed inter- and intracellularly for feeding sites. The aphids appeared preferentially to probe for and feed from thin-walled sieve tubes, as few thick-walled sieve tubes showed evidence of damage. Vessels, apparently probed for water, contained watery saliva that encased the secondary walls and sealed pit membranes between probed vessels and xylem parenchyma. The xylem probed by the RWA was rendered non-functional, probably contributing to symptoms of leaf roll, chlorosis and necrosis, which were observed within two weeks of infestation in the susceptible Betta cultivar. This damage was limited in the resistant Betta-Dn1 cultivar during the same time frame.
- Full Text:
- Date Issued: 2007
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