Knowledge, awareness, and molecular epidemiology of fasciolosis in dairy cattle slaughtered in three commercial abattoirs in Eastern Cape Province, South Africa
- Authors: Mpisana, Zuko
- Date: 2023-06
- Subjects: Farm management -- South Africa -- Eastern Cape , Dairy cattle -- South Africa -- Eastern Cape , Fascioliasis
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/28173 , vital:73792
- Description: Flukes and snail-borne diseases present significant challenges to livestock production, particularly impacting the dairy industry in the Eastern Cape Province, South Africa. Despite the challenges faced by the dairy sector, the level of knowledge and awareness among dairy farm personnel regarding the prevalence of bovine fasciolosis is unknown in Eastern Cape Province. Additionally, the genomic sequence of Fasciola spp. in dairy cattle slaughtered under abattoir conditions remains undisclosed. The primary objective of the titled study is to evaluate the knowledge, awareness, and molecular epidemiology of fasciolosis in dairy cattle slaughtered in three commercial abattoirs in the Eastern Cape province of South Africa. A structured questionnaire was prepared and randomly administered to 152 dairy farm personnel to investigate the knowledge and awareness of the aetiology, risk factors, and clinical signs of bovine fasciolosis among dairy farm personnel in the Eastern Cape Province, South Africa. A higher proportion of respondents from coastal regions were knowledgeable about the aetiology P equals 0.001 and the intermediate host P equals 0.000 of fasciolosis than those from the inland farms. A higher proportion of dairy farm personnel were not knowledgeable about the clinical signs of fasciolosis P equals 0.000 and the zoonotic potential P equals 0.001 of the disease. All farm personnel knew that season P 0.001 is a risk factor associated with snail-borne disease and occur throughout the year. However, the majority 63.2 percent of farm personnel agreed that fasciolosis was more prevalent in the summer followed by spring 27.6 percent, autumn 4.7 percent and winter 4.7 percent. Experiment 2 examined the body condition scores, fluke intensity, liver pathology, and carcass quality of various dairy cattle genotypes affected by Fasciola spp. infection, within three high-capacity abattoirs located in the Eastern Cape Province, South Africa. Milder infections were significantly noted to occur at a higher rate P 0.05 in Cross-bred cattle 46.9 percent followed by Holstein Friesian 39.5 percent and Jersey 30.9 percent cattle. Conversely, moderate infections were more prevalent P 0.05 among Jersey cattle 28.0 percent followed by Holstein Friesian 15.4 percent and Cross bred 1.9 percent cattle. Severe infections were more frequent P 0.05 in Holstein-Friesian 45.1 percent, followed by Jersey 41.9 percent and then Cross-bred 31.7 percent cattle. Heavy infections were observed in cattle with poor body condition scores in CA3. Mild infections were observed more in cattle with moderate body condition scores in CA3 and CA1 and less in CA2. Chapter 5 compared the detection rate of Fasciola infections using four different methods real-time quantitative polymerase chain reaction qPCR faecal sedimentation, antibody enzyme linked immunosorbent assay Ab ELISA and post-mortem liver examination among naturally infected cattle slaughtered at the abattoirs in the Eastern Cape Province, South Africa. A total of 145 slaughter cattle were screened for Fasciola infestation by liver examination, and corresponding samples from individual animals were analysed in the laboratory using Ab ELISA faecal sedimentation, and qPCR. The detection rate by liver inspection gold standard was significantly P 0.0001 higher than that by sedimentation and Ab ELISA but not significantly P 0.05 different from that by qPCR P equals 0.198. Post-mortem liver examination 94.5 percent and qPCR 90.4 percent had higher detection rate compared to Ab ELISA 22.6 percent and sedimentation 3.53 percent, respectively. The agreement between Ab ELISA and sedimentation was poor to slight kappa equals to minus 0.09 and with qPCR it was 0.008. The fourth experiment investigated genomic sequence of Fasciola spp. from faecal sample of slaughtered dairy cattle in Eastern Cape Province South Africa. Sequential and phylogenetic analyses were conducted to detect the presence of Fasciola spp. from the faecal samples obtained at the high throughput abattoirs. Fasciola hepatica and F. gigantica ITS 2 sequences were obtained by amplifying the 364 bp and 300 bp genes, respectively using species specific conventional PCR assays followed by cloning and sequencing. The phylogenetic tree revealed the presence of Fasciola hepatica as the only aetiological agent infecting dairy cattle in the Eastern Cape Province South Africa. The study concluded that dairy farm personnel lacks knowledge about zoonotic potentials, aetiological agents of fasciolosis high fluke intensity and its impact on carcasses sensitivity and suitability of detection methods. The study observed Fasciola hepatica as the only trematode spp. present in Eastern Cape Province South Africa. , Thesis (PhD) -- Faculty of Science and Agriculture, 2023
- Full Text:
- Date Issued: 2023-06
- Authors: Mpisana, Zuko
- Date: 2023-06
- Subjects: Farm management -- South Africa -- Eastern Cape , Dairy cattle -- South Africa -- Eastern Cape , Fascioliasis
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/28173 , vital:73792
- Description: Flukes and snail-borne diseases present significant challenges to livestock production, particularly impacting the dairy industry in the Eastern Cape Province, South Africa. Despite the challenges faced by the dairy sector, the level of knowledge and awareness among dairy farm personnel regarding the prevalence of bovine fasciolosis is unknown in Eastern Cape Province. Additionally, the genomic sequence of Fasciola spp. in dairy cattle slaughtered under abattoir conditions remains undisclosed. The primary objective of the titled study is to evaluate the knowledge, awareness, and molecular epidemiology of fasciolosis in dairy cattle slaughtered in three commercial abattoirs in the Eastern Cape province of South Africa. A structured questionnaire was prepared and randomly administered to 152 dairy farm personnel to investigate the knowledge and awareness of the aetiology, risk factors, and clinical signs of bovine fasciolosis among dairy farm personnel in the Eastern Cape Province, South Africa. A higher proportion of respondents from coastal regions were knowledgeable about the aetiology P equals 0.001 and the intermediate host P equals 0.000 of fasciolosis than those from the inland farms. A higher proportion of dairy farm personnel were not knowledgeable about the clinical signs of fasciolosis P equals 0.000 and the zoonotic potential P equals 0.001 of the disease. All farm personnel knew that season P 0.001 is a risk factor associated with snail-borne disease and occur throughout the year. However, the majority 63.2 percent of farm personnel agreed that fasciolosis was more prevalent in the summer followed by spring 27.6 percent, autumn 4.7 percent and winter 4.7 percent. Experiment 2 examined the body condition scores, fluke intensity, liver pathology, and carcass quality of various dairy cattle genotypes affected by Fasciola spp. infection, within three high-capacity abattoirs located in the Eastern Cape Province, South Africa. Milder infections were significantly noted to occur at a higher rate P 0.05 in Cross-bred cattle 46.9 percent followed by Holstein Friesian 39.5 percent and Jersey 30.9 percent cattle. Conversely, moderate infections were more prevalent P 0.05 among Jersey cattle 28.0 percent followed by Holstein Friesian 15.4 percent and Cross bred 1.9 percent cattle. Severe infections were more frequent P 0.05 in Holstein-Friesian 45.1 percent, followed by Jersey 41.9 percent and then Cross-bred 31.7 percent cattle. Heavy infections were observed in cattle with poor body condition scores in CA3. Mild infections were observed more in cattle with moderate body condition scores in CA3 and CA1 and less in CA2. Chapter 5 compared the detection rate of Fasciola infections using four different methods real-time quantitative polymerase chain reaction qPCR faecal sedimentation, antibody enzyme linked immunosorbent assay Ab ELISA and post-mortem liver examination among naturally infected cattle slaughtered at the abattoirs in the Eastern Cape Province, South Africa. A total of 145 slaughter cattle were screened for Fasciola infestation by liver examination, and corresponding samples from individual animals were analysed in the laboratory using Ab ELISA faecal sedimentation, and qPCR. The detection rate by liver inspection gold standard was significantly P 0.0001 higher than that by sedimentation and Ab ELISA but not significantly P 0.05 different from that by qPCR P equals 0.198. Post-mortem liver examination 94.5 percent and qPCR 90.4 percent had higher detection rate compared to Ab ELISA 22.6 percent and sedimentation 3.53 percent, respectively. The agreement between Ab ELISA and sedimentation was poor to slight kappa equals to minus 0.09 and with qPCR it was 0.008. The fourth experiment investigated genomic sequence of Fasciola spp. from faecal sample of slaughtered dairy cattle in Eastern Cape Province South Africa. Sequential and phylogenetic analyses were conducted to detect the presence of Fasciola spp. from the faecal samples obtained at the high throughput abattoirs. Fasciola hepatica and F. gigantica ITS 2 sequences were obtained by amplifying the 364 bp and 300 bp genes, respectively using species specific conventional PCR assays followed by cloning and sequencing. The phylogenetic tree revealed the presence of Fasciola hepatica as the only aetiological agent infecting dairy cattle in the Eastern Cape Province South Africa. The study concluded that dairy farm personnel lacks knowledge about zoonotic potentials, aetiological agents of fasciolosis high fluke intensity and its impact on carcasses sensitivity and suitability of detection methods. The study observed Fasciola hepatica as the only trematode spp. present in Eastern Cape Province South Africa. , Thesis (PhD) -- Faculty of Science and Agriculture, 2023
- Full Text:
- Date Issued: 2023-06
Primiparous and multiparous cow behaviour of friesland, jersey and cross-bred cows around calving time and during milking
- Authors: Mpisana, Zuko
- Date: 2015
- Subjects: Dairy products--Analysis Jersey cattle Milk yield
- Language: English
- Type: Thesis , Masters , Animal Science
- Identifier: http://hdl.handle.net/10353/16113 , vital:40665
- Description: The objective of the study was to determine effect of behaviour of primiparous and multiparous Friesland, Jersey and Cross-bred cows around calving time and during milking of first lactating cows in a pasture-based dairy system. The trial was conducted at the Fort Hare dairy farm. All pregnant primiparous and multiparous were kept in a maternity paddock. Five observers monitored the cows from the onset of parturition until the calf was fully expelled using digital stop watches. The behavioural responses of the cows during parturition (frequency and duration of lying, standing and walking bouts; calf licking; suckling) and milking (avoidance distance scores (ADS), entrance score, (ES), exit speed (ES), feeding score (FS), and the occurrence of stepping (STP) and kicking (KCK)) were determined on eighty consecutive days. The behavioural attributes were analysed using generalised linear model of procedure of SAS (2003). One hundred and twenty pregnant cows were used in the study, comprising of forty cows per genotype in different parities (A-primiparous, B- 2 to 4, C-5 to 6 and D-7 to 8) and kept in a maternity paddock. Five observers monitored cows from the onset of parturition until the calf was fully expelled. There were differences (P < 0.05) observed on the behavioural patterns around the time of calving. Jersey multiparous cows spent (P < 0.05) significantly most of their time (20.50±3.10) in lying position as compared to the other genotypes. The Jersey genotype also spent most (P < 0.05) of their time (48.00±0.34) in a standing position during calving period. Friesland cows spent (P < 0.05) most of their time (12.00±1.19) exhibiting either stepping or walking attributes as compared to Jersey and Crossbred cows. The Jersey genotype spent significantly (P < 0.05) more time (123.00±10.43) expelling calves compared to the other genotypes. Crossbred cows had the highest avoidance distance (14percent) scores compared to other genotypes and the Friesland cows had the lowest distance scores. The occurrence of kicking was significantly (P < 0.05) higher for the Friesland cows (57.3percent) compared to Jersey (23.2percent) and Crossbred cows (19.6percent). The Friesland cows had the highest (P < 0.05) frequency (36.2percent) feeding score compared to Jersey and Crossbred cows. The Jersey cows had the highest (60.2percent) exit speed compared to other genotypes. The occurrence of stepping was higher for the Friesland cows (53.9percent). The Friesland cows had the highest (P < 0.05) daily milk yield compared to Jersey and Crossbred cows. The present study revealed that behavioural activities of dairy cattle during parturition differed according to genotype and parity and the behavioural response of first lactating cows differ according to genotype during milking.
- Full Text:
- Date Issued: 2015
- Authors: Mpisana, Zuko
- Date: 2015
- Subjects: Dairy products--Analysis Jersey cattle Milk yield
- Language: English
- Type: Thesis , Masters , Animal Science
- Identifier: http://hdl.handle.net/10353/16113 , vital:40665
- Description: The objective of the study was to determine effect of behaviour of primiparous and multiparous Friesland, Jersey and Cross-bred cows around calving time and during milking of first lactating cows in a pasture-based dairy system. The trial was conducted at the Fort Hare dairy farm. All pregnant primiparous and multiparous were kept in a maternity paddock. Five observers monitored the cows from the onset of parturition until the calf was fully expelled using digital stop watches. The behavioural responses of the cows during parturition (frequency and duration of lying, standing and walking bouts; calf licking; suckling) and milking (avoidance distance scores (ADS), entrance score, (ES), exit speed (ES), feeding score (FS), and the occurrence of stepping (STP) and kicking (KCK)) were determined on eighty consecutive days. The behavioural attributes were analysed using generalised linear model of procedure of SAS (2003). One hundred and twenty pregnant cows were used in the study, comprising of forty cows per genotype in different parities (A-primiparous, B- 2 to 4, C-5 to 6 and D-7 to 8) and kept in a maternity paddock. Five observers monitored cows from the onset of parturition until the calf was fully expelled. There were differences (P < 0.05) observed on the behavioural patterns around the time of calving. Jersey multiparous cows spent (P < 0.05) significantly most of their time (20.50±3.10) in lying position as compared to the other genotypes. The Jersey genotype also spent most (P < 0.05) of their time (48.00±0.34) in a standing position during calving period. Friesland cows spent (P < 0.05) most of their time (12.00±1.19) exhibiting either stepping or walking attributes as compared to Jersey and Crossbred cows. The Jersey genotype spent significantly (P < 0.05) more time (123.00±10.43) expelling calves compared to the other genotypes. Crossbred cows had the highest avoidance distance (14percent) scores compared to other genotypes and the Friesland cows had the lowest distance scores. The occurrence of kicking was significantly (P < 0.05) higher for the Friesland cows (57.3percent) compared to Jersey (23.2percent) and Crossbred cows (19.6percent). The Friesland cows had the highest (P < 0.05) frequency (36.2percent) feeding score compared to Jersey and Crossbred cows. The Jersey cows had the highest (60.2percent) exit speed compared to other genotypes. The occurrence of stepping was higher for the Friesland cows (53.9percent). The Friesland cows had the highest (P < 0.05) daily milk yield compared to Jersey and Crossbred cows. The present study revealed that behavioural activities of dairy cattle during parturition differed according to genotype and parity and the behavioural response of first lactating cows differ according to genotype during milking.
- Full Text:
- Date Issued: 2015
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