Researcher Profile and List of Publications Dominic Abaver.pdf
- Authors: Abaver, Dominic, Targema
- Identifier: http://hdl.handle.net/11260/4799 , vital:44256
- Description:
Expertise:
Public Health/Medical Microbiology
- Full Text:
- Authors: Abaver, Dominic, Targema
- Identifier: http://hdl.handle.net/11260/4799 , vital:44256
- Description:
Expertise:
Public Health/Medical Microbiology
- Full Text:
ajphes.2021.27.4.5.pdf
- Authors: Adelin Kantore
- Identifier: http://hdl.handle.net/11260/7445 , vital:53979
- Full Text:
- Authors: Adelin Kantore
- Identifier: http://hdl.handle.net/11260/7445 , vital:53979
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The association between HIV tri-therapy with the development of Type-2 Diabetes Mellitus in a rural South African district: A case-control study
- Bam, Nokwanda E, Mabunda, Sikhumbuzo A, Ntsaba, Jafta, Apalata, Teke, Monatshila, Sibusisu C, Chitha, Wesile
- Authors: Bam, Nokwanda E , Mabunda, Sikhumbuzo A , Ntsaba, Jafta , Apalata, Teke , Monatshila, Sibusisu C , Chitha, Wesile
- Date: 2020
- Subjects: Type 2 diabetes , AIDS (Disease) , Rural health
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/11260/4008 , vital:43983 , https://doi.org/10.1371/journal.pone.0244067
- Description: Combination antiretroviral drugs(cARVs)prolong patients’ lives but are unfortunately thought to increase complications related to metabolic disorders including type-2 Diabetes Mellitus(DM).We sought to confirm the association of cARVs with type-2DM and ascertain the extent of this association in a rural South African setting. A case-control study of 177 (33.33%) cases with HIV/AIDS and type-2 DM were selected and compared with 354 (66.67%) non-DM HIV/AIDS unmatched controls from a rural district of South Africa’s third most populous province (Eastern Cape). Cases were identified from community health centres using the district health information system, and controls were identified using simple random sampling from the same health facilities. Odds Ratios (OR), together with 95% confidence intervals, were calculated for all the univariable and multivariable logistic analyses. This study found that cARVs significantly increased the occurrence of type-2 DM among HIV patients. Patients on protease inhibitors (PIs) were at least 21 times significantly more likely to be diabetic than those on the fixed dose combination (FDC); those on stavudine (D4T) and zidovudine (AZT) were 2.45 times and 9.44 times respectively more likely to be diabetic than those on FDC. The odds of diabetes increased by more than three-folds for those who had been on antiretroviral drugs for more than 6 years.
- Full Text:
- Date Issued: 2020
- Authors: Bam, Nokwanda E , Mabunda, Sikhumbuzo A , Ntsaba, Jafta , Apalata, Teke , Monatshila, Sibusisu C , Chitha, Wesile
- Date: 2020
- Subjects: Type 2 diabetes , AIDS (Disease) , Rural health
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/11260/4008 , vital:43983 , https://doi.org/10.1371/journal.pone.0244067
- Description: Combination antiretroviral drugs(cARVs)prolong patients’ lives but are unfortunately thought to increase complications related to metabolic disorders including type-2 Diabetes Mellitus(DM).We sought to confirm the association of cARVs with type-2DM and ascertain the extent of this association in a rural South African setting. A case-control study of 177 (33.33%) cases with HIV/AIDS and type-2 DM were selected and compared with 354 (66.67%) non-DM HIV/AIDS unmatched controls from a rural district of South Africa’s third most populous province (Eastern Cape). Cases were identified from community health centres using the district health information system, and controls were identified using simple random sampling from the same health facilities. Odds Ratios (OR), together with 95% confidence intervals, were calculated for all the univariable and multivariable logistic analyses. This study found that cARVs significantly increased the occurrence of type-2 DM among HIV patients. Patients on protease inhibitors (PIs) were at least 21 times significantly more likely to be diabetic than those on the fixed dose combination (FDC); those on stavudine (D4T) and zidovudine (AZT) were 2.45 times and 9.44 times respectively more likely to be diabetic than those on FDC. The odds of diabetes increased by more than three-folds for those who had been on antiretroviral drugs for more than 6 years.
- Full Text:
- Date Issued: 2020
Molecular Detection of Antibiotic-Resistant Genes in Pseudomonas aeruginosa from Nonclinical Environment: Public Health Implications in Mthatha, Eastern Cape Province, South Africa
- Mojisola Clara Hosu, Sandeep Vasaikar, Grace Emily Okuthe, Teke Apalata
- Authors: Mojisola Clara Hosu , Sandeep Vasaikar , Grace Emily Okuthe , Teke Apalata
- Date: 5 January 2021
- Subjects: Microbiology
- Language: English
- Type: Journal Article
- Identifier: http://hdl.handle.net/11260/2417 , vital:41877
- Description: Evaluation of resistant profiles and detection of antimicrobial-resistant genes of bacterial pathogens in the nonclinical milieu is imperative to assess the probable risk of dissemination of resistant genes in the environment. .is paper sought to identify antibiotic-resistant genes in Pseudomonas aeruginosa from nonclinical sources in Mthatha, Eastern Cape, and evaluate its public health implications. Samples collected from abattoir wastewater and aquatic environment were processed by membrane filtration and cultured on CHROMagarTM Pseudomonas medium. Species identification was performed by autoSCAN-4 (Dade Behring Inc., IL). Molecular characterization of the isolates was confirmed using real-time polymerase chain reaction (rPCR) and selected isolates were further screened for the possibility of harboring antimicrobial resistance genes. Fifty-one Pseudomonas species were recovered from abattoir wastewater and surface water samples, out of which thirty-six strains were Pseudomonas aeruginosa (70.6%). .e P. aeruginosa isolates demonstrated resistance to aztreonam (86.1%), ceftazidime (63.9%), piperacillin (58.3%), cefepime (55.6%), imipenem (50%), piperacillin/tazobactam (47.2%), meropenem (41.7%), and levofloxacin (30.6%). Twenty out of thirty-six P. aeruginosa displayed multidrug resistance profiles and were classified as multidrug-resistant (MDR) (55.6%). Most of the bacterial isolates exhibited a high Multiple Antibiotic Resistance (MAR) Index ranging from 0.08 to 0.69 with a mean MAR index of 0.38. In the rPCR analysis of fifteen P. aeruginosa isolates, 14 isolates (93.3%) were detected harboring blaSHV, six isolates (40%) harbored blaTEM, and three isolates (20%) harbored blaCTX-M, being the least occurring ESBL. Results of the current study revealed that P. aeruginosa isolates recovered from nonclinical milieu are resistant to frontline clinically relevant antipseudomonal drugs. .is is concerning as it poses a risk to the environment and constitutes a public health threat. Given the public health relevance, the paper recommends monitoring of multidrug-resistant pathogens in effluent environments.
- Full Text:
- Authors: Mojisola Clara Hosu , Sandeep Vasaikar , Grace Emily Okuthe , Teke Apalata
- Date: 5 January 2021
- Subjects: Microbiology
- Language: English
- Type: Journal Article
- Identifier: http://hdl.handle.net/11260/2417 , vital:41877
- Description: Evaluation of resistant profiles and detection of antimicrobial-resistant genes of bacterial pathogens in the nonclinical milieu is imperative to assess the probable risk of dissemination of resistant genes in the environment. .is paper sought to identify antibiotic-resistant genes in Pseudomonas aeruginosa from nonclinical sources in Mthatha, Eastern Cape, and evaluate its public health implications. Samples collected from abattoir wastewater and aquatic environment were processed by membrane filtration and cultured on CHROMagarTM Pseudomonas medium. Species identification was performed by autoSCAN-4 (Dade Behring Inc., IL). Molecular characterization of the isolates was confirmed using real-time polymerase chain reaction (rPCR) and selected isolates were further screened for the possibility of harboring antimicrobial resistance genes. Fifty-one Pseudomonas species were recovered from abattoir wastewater and surface water samples, out of which thirty-six strains were Pseudomonas aeruginosa (70.6%). .e P. aeruginosa isolates demonstrated resistance to aztreonam (86.1%), ceftazidime (63.9%), piperacillin (58.3%), cefepime (55.6%), imipenem (50%), piperacillin/tazobactam (47.2%), meropenem (41.7%), and levofloxacin (30.6%). Twenty out of thirty-six P. aeruginosa displayed multidrug resistance profiles and were classified as multidrug-resistant (MDR) (55.6%). Most of the bacterial isolates exhibited a high Multiple Antibiotic Resistance (MAR) Index ranging from 0.08 to 0.69 with a mean MAR index of 0.38. In the rPCR analysis of fifteen P. aeruginosa isolates, 14 isolates (93.3%) were detected harboring blaSHV, six isolates (40%) harbored blaTEM, and three isolates (20%) harbored blaCTX-M, being the least occurring ESBL. Results of the current study revealed that P. aeruginosa isolates recovered from nonclinical milieu are resistant to frontline clinically relevant antipseudomonal drugs. .is is concerning as it poses a risk to the environment and constitutes a public health threat. Given the public health relevance, the paper recommends monitoring of multidrug-resistant pathogens in effluent environments.
- Full Text:
The association between HIV tri-therapy with the development of Type-2 Diabetes Mellitus in a rural South African District: A case-control study
- Nokwanda E. Bam, Teke Apalata, Sikhumbuzo A. Mabunda, Jafta Ntsaba, Sibusiso C. Nomatshila, Wezile Chitha
- Authors: Nokwanda E. Bam , Teke Apalata , Sikhumbuzo A. Mabunda , Jafta Ntsaba , Sibusiso C. Nomatshila , Wezile Chitha
- Language: English
- Type: Journal Article
- Identifier: http://hdl.handle.net/11260/4667 , vital:44155
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- Authors: Nokwanda E. Bam , Teke Apalata , Sikhumbuzo A. Mabunda , Jafta Ntsaba , Sibusiso C. Nomatshila , Wezile Chitha
- Language: English
- Type: Journal Article
- Identifier: http://hdl.handle.net/11260/4667 , vital:44155
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Species distribution and antifungal susceptibility patterns of Candida isolates from a public tertiary teaching hospital in the Eastern Cape Province, South Africa
- Mnge, P, Okeleye, B I, Vasaikar, S D, Apalata, T
- Authors: Mnge, P , Okeleye, B I , Vasaikar, S D , Apalata, T
- Date: 2017-03-14
- Subjects: Candida Candida species , Antifungal agents Antifungal susceptibility
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/11260/6817 , vital:49389 , http://dx.doi.org/10.1590/1414-431X20175797
- Description: Candida species are the leading cause of invasive fungal infections, and over the past decade there has been an increased isolation of drug resistant Candida species. This study aimed to identify the species distribution of Candida isolates and to determine their unique antifungal susceptibility and resistance patterns. During a cross-sectional study, 209 Candida isolates (recovered from 206 clinical samples) were collected and their species distribution was determined using ChromAgar Candida. The Vitek-2 system (Biomerieux, South Africa) was used to determine minimum inhibitory concentrations (MICs) to azoles (fluconazole, voriconazole), echinocandins (caspofungin, micafungin), polyenes (amphotericin B) and flucytosine. Four species of Candida were isolated, of which C. albicans was the most frequent, isolated in 45.4 percent (95/209) of the isolates, followed by C. glabrata: 31.1 percent (65/209). The MICs of the different antifungal drugs varied amongst the species of Candida. From the 130 isolates tested for MICs, 90.77 percent (112/130) were susceptible to all antifungal drugs and 6.9 percent (9/130) of the isolates were multi-drug resistant. C. dubliniensis (n=2) isolates were susceptible to all the above mentioned antifungal drugs. There was no significant difference in species distribution amongst clinical specimens and between patients’ genders (P40.05). An increase in MIC values for fluconazole and flucytosine towards the resistance range was observed. To our knowledge, this is the first report on surveillance of Candida species distribution and antifungal susceptibility at a public tertiary teaching hospital in Eastern Cape, South Africa.
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- Date Issued: 2017-03-14
- Authors: Mnge, P , Okeleye, B I , Vasaikar, S D , Apalata, T
- Date: 2017-03-14
- Subjects: Candida Candida species , Antifungal agents Antifungal susceptibility
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/11260/6817 , vital:49389 , http://dx.doi.org/10.1590/1414-431X20175797
- Description: Candida species are the leading cause of invasive fungal infections, and over the past decade there has been an increased isolation of drug resistant Candida species. This study aimed to identify the species distribution of Candida isolates and to determine their unique antifungal susceptibility and resistance patterns. During a cross-sectional study, 209 Candida isolates (recovered from 206 clinical samples) were collected and their species distribution was determined using ChromAgar Candida. The Vitek-2 system (Biomerieux, South Africa) was used to determine minimum inhibitory concentrations (MICs) to azoles (fluconazole, voriconazole), echinocandins (caspofungin, micafungin), polyenes (amphotericin B) and flucytosine. Four species of Candida were isolated, of which C. albicans was the most frequent, isolated in 45.4 percent (95/209) of the isolates, followed by C. glabrata: 31.1 percent (65/209). The MICs of the different antifungal drugs varied amongst the species of Candida. From the 130 isolates tested for MICs, 90.77 percent (112/130) were susceptible to all antifungal drugs and 6.9 percent (9/130) of the isolates were multi-drug resistant. C. dubliniensis (n=2) isolates were susceptible to all the above mentioned antifungal drugs. There was no significant difference in species distribution amongst clinical specimens and between patients’ genders (P40.05). An increase in MIC values for fluconazole and flucytosine towards the resistance range was observed. To our knowledge, this is the first report on surveillance of Candida species distribution and antifungal susceptibility at a public tertiary teaching hospital in Eastern Cape, South Africa.
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- Date Issued: 2017-03-14
The Role of T Helper 17 (Th17) and Regulatory T Cells (Treg) in the Pathogenesis of Vulvovaginal Candidiasis among HIV-Infected Women
- Teke Apalata, Benjamin Longo-Mbenza
- Authors: Teke Apalata , Benjamin Longo-Mbenza
- Date: 2020
- Language: English
- Type: Journal Article
- Identifier: http://hdl.handle.net/11260/4363 , vital:44109
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- Authors: Teke Apalata , Benjamin Longo-Mbenza
- Date: 2020
- Language: English
- Type: Journal Article
- Identifier: http://hdl.handle.net/11260/4363 , vital:44109
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In vitro antimicrobial photodynamic inactivation of multidrugresistant Acinetobacter baumannii biofilm using Protoporphyrin IX and Methylene blue
- Anane, Yaw Adjei, Apalata, Teke, Vasaikar, Sandeep, Okuthe, Grace Emily, Songca, Sandile Phinda
- Authors: Anane, Yaw Adjei , Apalata, Teke , Vasaikar, Sandeep , Okuthe, Grace Emily , Songca, Sandile Phinda
- Date: 2020
- Language: English
- Identifier: http://hdl.handle.net/11260/2273 , vital:41315
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- Authors: Anane, Yaw Adjei , Apalata, Teke , Vasaikar, Sandeep , Okuthe, Grace Emily , Songca, Sandile Phinda
- Date: 2020
- Language: English
- Identifier: http://hdl.handle.net/11260/2273 , vital:41315
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Molecular Detection of Carbapenemase-Encoding Genes in Multidrug-Resistant Acinetobacter baumannii Clinical Isolates in South Africa
- Anane, Yaw Adjei, Apalata, Teke, Vasaikar, Sandeep, Okuthe, Grace Emily, Songca, Sandile
- Authors: Anane, Yaw Adjei , Apalata, Teke , Vasaikar, Sandeep , Okuthe, Grace Emily , Songca, Sandile
- Date: 2020
- Language: English
- Type: Journal Article
- Identifier: http://hdl.handle.net/11260/2263 , vital:41314
- Description: https://www.hindawi.com/journals/ijmicro/2020/7380740/
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- Authors: Anane, Yaw Adjei , Apalata, Teke , Vasaikar, Sandeep , Okuthe, Grace Emily , Songca, Sandile
- Date: 2020
- Language: English
- Type: Journal Article
- Identifier: http://hdl.handle.net/11260/2263 , vital:41314
- Description: https://www.hindawi.com/journals/ijmicro/2020/7380740/
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Molecular Detection of Carbapenemase-Encoding Genes in Multidrug-Resistant Acinetobacter baumannii Clinical Isolates in South Africa
- Anane, Yaw Adjei, Okuthe, Grace Emily, Apalata, Teke, Vasaikar, Sandeep, Songca, Sandile
- Authors: Anane, Yaw Adjei , Okuthe, Grace Emily , Apalata, Teke , Vasaikar, Sandeep , Songca, Sandile
- Date: 2020
- Language: English
- Type: Journal Article
- Identifier: http://hdl.handle.net/11260/4351 , vital:44108
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- Authors: Anane, Yaw Adjei , Okuthe, Grace Emily , Apalata, Teke , Vasaikar, Sandeep , Songca, Sandile
- Date: 2020
- Language: English
- Type: Journal Article
- Identifier: http://hdl.handle.net/11260/4351 , vital:44108
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Outcomes and Risk Factors Associated with Drug Resistant Tuberculosis in Rural Eastern Cape, South Africa.pdf
- Identifier: http://hdl.handle.net/11260/5058 , vital:44312
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- Identifier: http://hdl.handle.net/11260/5058 , vital:44312
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Detection of extended spectrum beta‑lactamase genes in Pseudomonas aeruginosa isolated from patients in rural Eastern Cape Province, South Africa
- Mojisola C. Hosu, Sandeep D. Vasaikar,, Grace E. Okuthe, Teke Apalata
- Authors: Mojisola C. Hosu , Sandeep D. Vasaikar, , Grace E. Okuthe , Teke Apalata
- Date: 2021
- Language: English
- Type: Journal Article
- Identifier: http://hdl.handle.net/11260/4509 , vital:44128
- Description: The proliferation of extended spectrum beta-lactamase (ESBL) producing Pseudomonas aeruginosa represent a major public health threat. In this study, we evaluated the antimicrobial resistance patterns of P. aeruginosa strains and characterized the ESBLs and Metallo- β-lactamases (MBL) produced. Strains of P. aeruginosa cultured from patients who attended Nelson Mandela Academic Hospital and other clinics in the four district municipalities of the Eastern Cape between August 2017 and May 2019 were identified; antimicrobial susceptibility testing was carried out against thirteen clinically relevant antibiotics using the BioMérieux VITEK 2 and confirmed by Beckman autoSCAN-4 System. Real-time PCR was done using Roche Light Cycler 2.0 to detect the presence of ESBLs; blaSHV, blaTEM and blaCTX-M genes; and MBLs; blaIMP, blaVIM. Strains of P. aeruginosa demonstrated resistance to wide-ranging clinically relevant antibiotics including piperacillin (64.2%), followed by aztreonam (57.8%), cefepime (51.5%), ceftazidime (51.0%), piperacillin/tazobactam (50.5%), and imipenem (46.6%). A total of 75 (36.8%) multidrug-resistant (MDR) strains were observed of the total pool of isolates. The blaTEM, blaSHV and blaCTX-M was detected in 79.3%, 69.5% and 31.7% isolates (n = 82), respectively. The blaIMP was detected in 1.25% while no blaVIM was detected in any of the strains tested. The study showed a high rate of MDR P. aeruginosa in our setting. The vast majority of these resistant strains carried blaTEM and blaSHV genes. Continuous monitoring of antimicrobial resistance and strict compliance towards infection prevention and control practices are the best defence against spread of MDR P. aeruginosa.
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- Authors: Mojisola C. Hosu , Sandeep D. Vasaikar, , Grace E. Okuthe , Teke Apalata
- Date: 2021
- Language: English
- Type: Journal Article
- Identifier: http://hdl.handle.net/11260/4509 , vital:44128
- Description: The proliferation of extended spectrum beta-lactamase (ESBL) producing Pseudomonas aeruginosa represent a major public health threat. In this study, we evaluated the antimicrobial resistance patterns of P. aeruginosa strains and characterized the ESBLs and Metallo- β-lactamases (MBL) produced. Strains of P. aeruginosa cultured from patients who attended Nelson Mandela Academic Hospital and other clinics in the four district municipalities of the Eastern Cape between August 2017 and May 2019 were identified; antimicrobial susceptibility testing was carried out against thirteen clinically relevant antibiotics using the BioMérieux VITEK 2 and confirmed by Beckman autoSCAN-4 System. Real-time PCR was done using Roche Light Cycler 2.0 to detect the presence of ESBLs; blaSHV, blaTEM and blaCTX-M genes; and MBLs; blaIMP, blaVIM. Strains of P. aeruginosa demonstrated resistance to wide-ranging clinically relevant antibiotics including piperacillin (64.2%), followed by aztreonam (57.8%), cefepime (51.5%), ceftazidime (51.0%), piperacillin/tazobactam (50.5%), and imipenem (46.6%). A total of 75 (36.8%) multidrug-resistant (MDR) strains were observed of the total pool of isolates. The blaTEM, blaSHV and blaCTX-M was detected in 79.3%, 69.5% and 31.7% isolates (n = 82), respectively. The blaIMP was detected in 1.25% while no blaVIM was detected in any of the strains tested. The study showed a high rate of MDR P. aeruginosa in our setting. The vast majority of these resistant strains carried blaTEM and blaSHV genes. Continuous monitoring of antimicrobial resistance and strict compliance towards infection prevention and control practices are the best defence against spread of MDR P. aeruginosa.
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Prevalence and molecular analysis of multidrug-resistant Acinetobacter baumannii in the extra-hospital environment in Mthatha, South Africa
- Adjei, Anane Yaw, Apalata, Teke, Vasaikar, Sandeep, Okuthe, Grace Emily, Songca, Sandile
- Authors: Adjei, Anane Yaw , Apalata, Teke , Vasaikar, Sandeep , Okuthe, Grace Emily , Songca, Sandile
- Date: 2019
- Subjects: Acinetobacter baumannii , Carbapenemase-encoding genes , Multidrug-resistance , ISAba1 , intI1
- Language: English
- Type: article , text
- Identifier: http://hdl.handle.net/11260/2409 , vital:41457 , https://doi.org/10.1016/j.bjid.2019.09.004
- Description: The presence of Acinetobacter baumannii outside hospitals remains unclear. This study aimed to determine the prevalence of multidrug-resistance (MDR) A. baumannii in the extra-hospital environment in Mthatha, South Africa and to investigate the frequency of carbapenemase-encoding genes. Material and Methods: From August 2016 to July 2017 a total of 598 abattoir samples and 689 aquatic samples were collected and analyzed presumptively by cultural methods for the presence of A. baumannii using CHROMagarTM Acinetobacter medium. Species identification was performed by autoSCAN-4 (Dade Behring Inc., IL) and confirmed by the detection of their intrinsic blaOXA-51 gene. Confirmed MDR A. baumannii isolates were screened for the presence of carbapenemase-encoding genes, ISAba1 insertion sequence and integrase intI1. Results: In total, 248 (19.3%) Acinetobacter species were isolated. Acinetobacter. baumannii was detected in 183 (73.8%) of which 85 (46.4%) and 98 (53.6%) were recovered from abattoir and aquatic respectively. MDR A. baumannii was detected in 56.5% (48/85) abattoir isolates and 53.1% (52/98) aquatic isolates. Isolates showed high resistance to antimicrobials most frequently used to treat Acinetobacter infections such as piperacillin/tazobactam; abattoir (98% of isolates resistant), aquatic (94% of isolates resistant), ceftazidime (84%, 83%), ciprofloxacin (71%, 70%), amikacin (41%, 42%), imipenem (75%, 73%), and meropenem (74%, 71%). All the isolates were susceptible to tigecycline and colistin. All the isolates carried blaOXA-51-like. The blaOXA-23 was detected in 32 (66.7%) abattoir isolates and 11 (21.2%) aquatic isolates. The blaOXA-58-like was positive in 7 (14.6%) and 4 (7.7%) abattoir and aquatic isolates, respectively. Both groups of isolates lacked blaOXA-24-like, blaIMP-type, blaVIM-type, blaNDM-1, blaSIM, blaAmpC, ISAba1 and inI1. Isolates showed high level of Multiple Antibiotic Resistance Index (MARI) ranging from 0.20-0.52. Conclusion: Extra-hospital sources such as abattoir and aquatic environments may be a vehicle of spread of MDR A. baumannii strains in the community and hospital settings.
- Full Text:
- Date Issued: 2019
- Authors: Adjei, Anane Yaw , Apalata, Teke , Vasaikar, Sandeep , Okuthe, Grace Emily , Songca, Sandile
- Date: 2019
- Subjects: Acinetobacter baumannii , Carbapenemase-encoding genes , Multidrug-resistance , ISAba1 , intI1
- Language: English
- Type: article , text
- Identifier: http://hdl.handle.net/11260/2409 , vital:41457 , https://doi.org/10.1016/j.bjid.2019.09.004
- Description: The presence of Acinetobacter baumannii outside hospitals remains unclear. This study aimed to determine the prevalence of multidrug-resistance (MDR) A. baumannii in the extra-hospital environment in Mthatha, South Africa and to investigate the frequency of carbapenemase-encoding genes. Material and Methods: From August 2016 to July 2017 a total of 598 abattoir samples and 689 aquatic samples were collected and analyzed presumptively by cultural methods for the presence of A. baumannii using CHROMagarTM Acinetobacter medium. Species identification was performed by autoSCAN-4 (Dade Behring Inc., IL) and confirmed by the detection of their intrinsic blaOXA-51 gene. Confirmed MDR A. baumannii isolates were screened for the presence of carbapenemase-encoding genes, ISAba1 insertion sequence and integrase intI1. Results: In total, 248 (19.3%) Acinetobacter species were isolated. Acinetobacter. baumannii was detected in 183 (73.8%) of which 85 (46.4%) and 98 (53.6%) were recovered from abattoir and aquatic respectively. MDR A. baumannii was detected in 56.5% (48/85) abattoir isolates and 53.1% (52/98) aquatic isolates. Isolates showed high resistance to antimicrobials most frequently used to treat Acinetobacter infections such as piperacillin/tazobactam; abattoir (98% of isolates resistant), aquatic (94% of isolates resistant), ceftazidime (84%, 83%), ciprofloxacin (71%, 70%), amikacin (41%, 42%), imipenem (75%, 73%), and meropenem (74%, 71%). All the isolates were susceptible to tigecycline and colistin. All the isolates carried blaOXA-51-like. The blaOXA-23 was detected in 32 (66.7%) abattoir isolates and 11 (21.2%) aquatic isolates. The blaOXA-58-like was positive in 7 (14.6%) and 4 (7.7%) abattoir and aquatic isolates, respectively. Both groups of isolates lacked blaOXA-24-like, blaIMP-type, blaVIM-type, blaNDM-1, blaSIM, blaAmpC, ISAba1 and inI1. Isolates showed high level of Multiple Antibiotic Resistance Index (MARI) ranging from 0.20-0.52. Conclusion: Extra-hospital sources such as abattoir and aquatic environments may be a vehicle of spread of MDR A. baumannii strains in the community and hospital settings.
- Full Text:
- Date Issued: 2019
Researcher Profile and List of Publications - Oyedeji.pdf
- Authors: Oyedeji, Adebola Omowunmi
- Identifier: http://hdl.handle.net/11260/4791 , vital:44254
- Description: Expertise: Organic chemistry-Natural Product Chemist
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- Authors: Oyedeji, Adebola Omowunmi
- Identifier: http://hdl.handle.net/11260/4791 , vital:44254
- Description: Expertise: Organic chemistry-Natural Product Chemist
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Professor
- Authors: Oyedeji, Adebola Omowunmi
- Type: Researcher Profile
- Identifier: http://hdl.handle.net/11260/2721 , vital:42330
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- Authors: Oyedeji, Adebola Omowunmi
- Type: Researcher Profile
- Identifier: http://hdl.handle.net/11260/2721 , vital:42330
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Evaluating the Prevention of Mother to Child Transmission (PMTCT) of HIV Services in the Eastern Cape: subtitle if needed. If no subtitle follow instructions in manual
- Authors: Sigovana, Khuthala
- Date: 2019
- Language: English
- Type: Masters theses , text
- Identifier: http://hdl.handle.net/11260/3176 , vital:43182
- Description: Abstract text To effectively reduce HIV transmission to newborns requires a reduction of HIV prevalence among pregnant women and a full understanding of its epidemiology. There is however, a literature gap: few recent HIV studies focus on women attending antenatal care in rural areas in South Africa. A Cross-sectional study of women attending antenatal care in four Primary Care facilities was conducted. An interviewer-administered questionnaire to collect information on socio-demographic characteristics and medical history was used. Binomial logistic regression analyses were used to determine factors associated with HIV and to estimate the prevalence ratio (PR). The 95% confidence interval (CI) is used for precision of estimates; p≤0.05 for statistical significance. A total of 343 participants were recruited. Most participants (n = 341 or 99.42%; CI: 97.91 – 99.93) knew their HIV status. The antenatal HIV prevalence was 38.19% (CI: 33.17 – 43.48). Participants older than 40 years were 3.99 and 3.81 times more likely to be HIV positive compared to teenagers (PR = 3.99; CI: 1.11 – 14.30; pstxvalue = 0.034) and those in the 20-29 age group (PR = 3.81; CI: 1.07 – 13.60; pstxvaluevalue = 0.039) respectively; and this was statistically significant. Unemployed participants were 34% (PR = 1.34; CI: 1.16 – 1.55; p-value less than 0.0001) and 41 percent (PR = 1.41; CI: 1.23 – 1.61; p-value less than 0.0001) more likely to be HIV positive when compared to tertiary students and self-employed individuals respectively. The antenatal HIV prevalence remains high and is increasing in this population. However, findings suggest a changing and maturing epidemic. , Thesis (MMED) -- Faculty of Health Sciences, 2019
- Full Text:
- Date Issued: 2019
- Authors: Sigovana, Khuthala
- Date: 2019
- Language: English
- Type: Masters theses , text
- Identifier: http://hdl.handle.net/11260/3176 , vital:43182
- Description: Abstract text To effectively reduce HIV transmission to newborns requires a reduction of HIV prevalence among pregnant women and a full understanding of its epidemiology. There is however, a literature gap: few recent HIV studies focus on women attending antenatal care in rural areas in South Africa. A Cross-sectional study of women attending antenatal care in four Primary Care facilities was conducted. An interviewer-administered questionnaire to collect information on socio-demographic characteristics and medical history was used. Binomial logistic regression analyses were used to determine factors associated with HIV and to estimate the prevalence ratio (PR). The 95% confidence interval (CI) is used for precision of estimates; p≤0.05 for statistical significance. A total of 343 participants were recruited. Most participants (n = 341 or 99.42%; CI: 97.91 – 99.93) knew their HIV status. The antenatal HIV prevalence was 38.19% (CI: 33.17 – 43.48). Participants older than 40 years were 3.99 and 3.81 times more likely to be HIV positive compared to teenagers (PR = 3.99; CI: 1.11 – 14.30; pstxvalue = 0.034) and those in the 20-29 age group (PR = 3.81; CI: 1.07 – 13.60; pstxvaluevalue = 0.039) respectively; and this was statistically significant. Unemployed participants were 34% (PR = 1.34; CI: 1.16 – 1.55; p-value less than 0.0001) and 41 percent (PR = 1.41; CI: 1.23 – 1.61; p-value less than 0.0001) more likely to be HIV positive when compared to tertiary students and self-employed individuals respectively. The antenatal HIV prevalence remains high and is increasing in this population. However, findings suggest a changing and maturing epidemic. , Thesis (MMED) -- Faculty of Health Sciences, 2019
- Full Text:
- Date Issued: 2019
Socio-demographic associations of HIV among women attending antenatal care in selected rural primary care facilities in South Africa’s Eastern Cape province
- Sikhumbuzo A. Mabunda, Khuthala Sigovana, Wezile Chitha, Teke Apalata, Sibusiso Nomatshila
- Authors: Sikhumbuzo A. Mabunda , Khuthala Sigovana , Wezile Chitha , Teke Apalata , Sibusiso Nomatshila
- Date: 2021
- Language: English
- Type: Journal article
- Identifier: http://hdl.handle.net/11260/4112 , vital:44025
- Full Text:
- Authors: Sikhumbuzo A. Mabunda , Khuthala Sigovana , Wezile Chitha , Teke Apalata , Sibusiso Nomatshila
- Date: 2021
- Language: English
- Type: Journal article
- Identifier: http://hdl.handle.net/11260/4112 , vital:44025
- Full Text:
Phytochemical Analysis and Biological potential of leaves and peels of three Eastern Cape Citrus Species
- Authors: Matuka, Thandiswa
- Date: 2019
- Subjects: Phytochemical -- Analysis Biology -- Leaves and peels Citrus species -- Eastern Cape -- South Africa
- Language: English
- Type: Thesis , Masters , M Sc
- Identifier: http://hdl.handle.net/11260/1362 , vital:32263
- Description: Citrus sinensis, Citrus reticulata and Citrus clementine are citrus species that belong to a large family Rutaceae. Citrus leaves and peels are often regarded as waste products. In view of the environment pollution impact, the search for end use of citrus waste products for economic value and medicinal purposes was sort after. The aim of this study was to investigate chemical composition of citrus waste products (leaves and peels) essential oils and determine biological active volatile compounds from Citrus sinensis, Citrus reticulata and Citrus clementine Leaves and peels of Citrus sinensis, Citrus reticulata and Citrus clementine collected twice from citrus farm were extracted for essential oils using hydrodistillation method and analysed by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS) for plant samples collected in 2016 and, gas chromatography (GC) and gas chromatography-triple quadrupole-mass spectrometry (GC-MSMS) for plant samples collected in 2017. The oil yield for Citrus sinensis was 0.19 - 0.85 %v/w for fresh and dried leaf and peels for samples collected in 2016 and 0.26 – 0.87% v/w for those collected in 2017. Analysis of the essential oils analyses identified sabinene (20.4% GCMS, 22.6% GCMSMS), terpinen-4-ol (13.2% GCMS, 6.8% GCMSMS) and linalool (7.6% GCMS, 19.3% GCMSMS) as major compounds in fresh leaves essential oils while β-elemene (16.3% GCMS, 21.4% GCMSMS) and sabinene (10.7% GCMS, 20.8% GCMSMS) were major compounds characterized in dried leaves essential oils. Fresh and dried peels essential oils had limonene as the major constituent (49.6% GCMS, 87.1% GCMSMS) and (73.6% GCMS, 88.3% GCMSMS) respectively. Citrus reticulata essential oils percentage yield range between 0.44-0.80% v/w for fresh and dried leaf and peel oils. Analyses of the GCMS and GCMSMS chromatogram of the volatile oils analyses revealed linalool (33.4% GCMS, 19.6% GCMSMS), (+)-spathulenol (15.2% GCMS, 10.1% GCMSMS) and terpinene-4-ol (4.5% GCMS, 11.9% GCMSMS) as major compounds in fresh leaf essential oils while, sabinene (20.8% GCMS, 32.5% GCMSMS) and linalool (17.2% GCMS, 20.4% GCMSMS) were the major compounds found in dried leaf essential oils. Limonene (55.2%, 75.4% GCMS; 39.5%, 80.8% GCMSMS) was the major prominent compound identified in the C. reticulata peel essential oils in fresh and dried peels respectively, followed by linalool (12.8%, 2.9% GCMS; 48.4%, 7.4% GCMSMS). Percentage yields for the essential oils from C. clementine fresh and dried leaves and peels ranges from 0.40-0.62% v/w for. Fresh and dried leaves volatile oils had sabinene (22.7-27.1%) for GCMS and GCMSMS respectively), linalool (18.5- 20.9% GCMS and GCMSMS) and terpinene-4-ol (6.5- 11.8% GCMS and GCMSMS) as main compounds while fresh and dried peels oils were dominated by limonene (38.9- 86.5% GCMS and GCMSMS). Phytochemical screening experiment of the three citrus species leaves and peels aqueous extract reveal the prominent presence of flavonoids and phenolic compounds in high concentrations. C. reticulata and C. clementine peels and leaves volatile oils exhibited higher antioxidant activity as DPPH free radical scavengers and Ferric reducing power (FRAP) ability, when compared to C. sinensis essential oils which exhibited minimum antioxidant activity. Antioxidant activity of leaves and peels essential oils maybe due to the presence of oxygenated monoterpenes and high limonene content. C. sinensis peels oils showed non-toxic to extremely very low toxicity effect at highest dose 2000 mg/kg. C. reticulata and C. clementine peels oils showed toxicity effect in mice at high dose levels. It is suggested that citrus volatile oils could be safe to use at lower dose levels. Citrus species peels and leaves volatile compounds limonene linalool and sabinene exhibited antiinflammatory activity by significantly reducing rats paw oedema size induced by fresh egg albumin. The study suggests the use of Citrus waste products (leaves and peels) as natural antioxidants with antiinflammatory properties for medicinal value and volatile oils compounds for aroma in beverages and cosmetic industries.
- Full Text:
- Date Issued: 2019
- Authors: Matuka, Thandiswa
- Date: 2019
- Subjects: Phytochemical -- Analysis Biology -- Leaves and peels Citrus species -- Eastern Cape -- South Africa
- Language: English
- Type: Thesis , Masters , M Sc
- Identifier: http://hdl.handle.net/11260/1362 , vital:32263
- Description: Citrus sinensis, Citrus reticulata and Citrus clementine are citrus species that belong to a large family Rutaceae. Citrus leaves and peels are often regarded as waste products. In view of the environment pollution impact, the search for end use of citrus waste products for economic value and medicinal purposes was sort after. The aim of this study was to investigate chemical composition of citrus waste products (leaves and peels) essential oils and determine biological active volatile compounds from Citrus sinensis, Citrus reticulata and Citrus clementine Leaves and peels of Citrus sinensis, Citrus reticulata and Citrus clementine collected twice from citrus farm were extracted for essential oils using hydrodistillation method and analysed by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS) for plant samples collected in 2016 and, gas chromatography (GC) and gas chromatography-triple quadrupole-mass spectrometry (GC-MSMS) for plant samples collected in 2017. The oil yield for Citrus sinensis was 0.19 - 0.85 %v/w for fresh and dried leaf and peels for samples collected in 2016 and 0.26 – 0.87% v/w for those collected in 2017. Analysis of the essential oils analyses identified sabinene (20.4% GCMS, 22.6% GCMSMS), terpinen-4-ol (13.2% GCMS, 6.8% GCMSMS) and linalool (7.6% GCMS, 19.3% GCMSMS) as major compounds in fresh leaves essential oils while β-elemene (16.3% GCMS, 21.4% GCMSMS) and sabinene (10.7% GCMS, 20.8% GCMSMS) were major compounds characterized in dried leaves essential oils. Fresh and dried peels essential oils had limonene as the major constituent (49.6% GCMS, 87.1% GCMSMS) and (73.6% GCMS, 88.3% GCMSMS) respectively. Citrus reticulata essential oils percentage yield range between 0.44-0.80% v/w for fresh and dried leaf and peel oils. Analyses of the GCMS and GCMSMS chromatogram of the volatile oils analyses revealed linalool (33.4% GCMS, 19.6% GCMSMS), (+)-spathulenol (15.2% GCMS, 10.1% GCMSMS) and terpinene-4-ol (4.5% GCMS, 11.9% GCMSMS) as major compounds in fresh leaf essential oils while, sabinene (20.8% GCMS, 32.5% GCMSMS) and linalool (17.2% GCMS, 20.4% GCMSMS) were the major compounds found in dried leaf essential oils. Limonene (55.2%, 75.4% GCMS; 39.5%, 80.8% GCMSMS) was the major prominent compound identified in the C. reticulata peel essential oils in fresh and dried peels respectively, followed by linalool (12.8%, 2.9% GCMS; 48.4%, 7.4% GCMSMS). Percentage yields for the essential oils from C. clementine fresh and dried leaves and peels ranges from 0.40-0.62% v/w for. Fresh and dried leaves volatile oils had sabinene (22.7-27.1%) for GCMS and GCMSMS respectively), linalool (18.5- 20.9% GCMS and GCMSMS) and terpinene-4-ol (6.5- 11.8% GCMS and GCMSMS) as main compounds while fresh and dried peels oils were dominated by limonene (38.9- 86.5% GCMS and GCMSMS). Phytochemical screening experiment of the three citrus species leaves and peels aqueous extract reveal the prominent presence of flavonoids and phenolic compounds in high concentrations. C. reticulata and C. clementine peels and leaves volatile oils exhibited higher antioxidant activity as DPPH free radical scavengers and Ferric reducing power (FRAP) ability, when compared to C. sinensis essential oils which exhibited minimum antioxidant activity. Antioxidant activity of leaves and peels essential oils maybe due to the presence of oxygenated monoterpenes and high limonene content. C. sinensis peels oils showed non-toxic to extremely very low toxicity effect at highest dose 2000 mg/kg. C. reticulata and C. clementine peels oils showed toxicity effect in mice at high dose levels. It is suggested that citrus volatile oils could be safe to use at lower dose levels. Citrus species peels and leaves volatile compounds limonene linalool and sabinene exhibited antiinflammatory activity by significantly reducing rats paw oedema size induced by fresh egg albumin. The study suggests the use of Citrus waste products (leaves and peels) as natural antioxidants with antiinflammatory properties for medicinal value and volatile oils compounds for aroma in beverages and cosmetic industries.
- Full Text:
- Date Issued: 2019
Prevalence of Hypertension and Its Associated Risk Factors in a Rural Black Population of Mthatha Town, South Africa
- Jyoti Rajan Sharma, Sihle E. Mabhida, Bronwyn Myers 3, Teke Apalata, Edward Nicol, Mongi Benjeddou, Christo Muller, Rabia Johnson
- Authors: Jyoti Rajan Sharma , Sihle E. Mabhida , Bronwyn Myers 3 , Teke Apalata , Edward Nicol , Mongi Benjeddou , Christo Muller , Rabia Johnson
- Date: 2021
- Language: English
- Type: Journal article
- Identifier: http://hdl.handle.net/11260/3980 , vital:43979
- Full Text:
- Authors: Jyoti Rajan Sharma , Sihle E. Mabhida , Bronwyn Myers 3 , Teke Apalata , Edward Nicol , Mongi Benjeddou , Christo Muller , Rabia Johnson
- Date: 2021
- Language: English
- Type: Journal article
- Identifier: http://hdl.handle.net/11260/3980 , vital:43979
- Full Text:
Mthatha Research and Innovation Day: programme
- Authors: Walter Sisulu University
- Date: 2021-08-15
- Subjects: Mthatha Research and Innovation Day
- Language: English
- Type: lecture , text
- Identifier: http://hdl.handle.net/11260/6267 , vital:45361
- Description: Mthatha Research and Innovation Day. Topic The Impact of the pandemic in Higher Education and Research: Challenges and possibilities.
- Full Text:
- Date Issued: 2021-08-15
- Authors: Walter Sisulu University
- Date: 2021-08-15
- Subjects: Mthatha Research and Innovation Day
- Language: English
- Type: lecture , text
- Identifier: http://hdl.handle.net/11260/6267 , vital:45361
- Description: Mthatha Research and Innovation Day. Topic The Impact of the pandemic in Higher Education and Research: Challenges and possibilities.
- Full Text:
- Date Issued: 2021-08-15