Potential impact and host range of Pereskiophaga brasiliensis Anderson (Curculionidae): a new candidate biological control agent for the control of Pereskia aculeata Miller (Cactaceae) in South Africa
- Authors: Mdodana, Lumka Anita
- Date: 2018
- Subjects: Curculionidae -- South Africa , Cactus -- South Africa , Biological pest control agents , Alien plants-- South Africa , Pereskiophaga brasiliensis Anderson (Curculionidae) , Pereskia aculeata Miller (Cactaceae)
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/62350 , vital:28157
- Description: Pereskia aculeata Miller (Cactaceae) is a damaging invasive alien plant in South Africa that has negative impacts to indigenous biodiversity and ecosystem functioning. Mechanical and chemical control are not effective against P. aculeata so biological control is considered the only viable option. Two biological control agents, the leaf-feeding beetle Phenrica guerini Bechyne (Chrysomelidae) and the stem-wilting bug Catorhintha schaffneri (Coreidae), have been released in South Africa thus far. Post-release evaluations have indicated that P. guerini will not reduce P. aculeata densities to acceptable levels alone, while C. schaffneri was released very recently, so it is too soon to determine how effective that agent will be. Even if C. schaffneri is extremely damaging, it is likely that further agents will be required to reduce the densities of P. aculeata to acceptable levels within a reasonable time-scale. Additional agents should target the woody stems of P. aculeata which are not impacted by the damage of either of the released agents. Pereskiophaga brasiliensis Anderson (Curculionidae) is a promising potential candidate agent that feeds on the thick woody stems of the plant in the larval stage. Climatic matching, genetic matching and field based host specificity observations all indicated that P. brasiliensis was a promising candidate. In this study, the impact of P. brasiliensis to the target weed, P. aculeata, was quantified under quarantine conditions to determine whether it was sufficiently damaging to warrant release. This was followed by host specificity testing to determine whether P. brasiliensis was suitably host specific for release in South Africa. Impact studies indicated that P. brasiliensis was damaging to P. aculeata at insect densities that would be expected in the field. Pereskiophaga brasiliensis reduced the number of leaves of P. aculeata to a greater extent than it reduced shoot lengths, but both plant parameters were significantly reduced due to the feeding damage from the insect. This suggests that the damage from P. brasiliensis may be compatible with that of C. schaffneri which reduces shoot length to a greater degree than the number of leaves. Pereskiophaga brasiliensis is therefore sufficiently damaging to warrant release, and although interaction studies with the other agents would be required, it is expected that it should complement other existing agents. Although P. brasiliensis is sufficiently damaging, at present the host specificity data indicates that it is not suitably specific for release in South Africa because oviposition and larval development to the adult stage was recorded on both indigenous and alien plant species within the families Cactaceae and Basellaceae. This non-target feeding was recorded during no-choice tests, which are very conservative, but significant non-target damage and development to the adult stage was recorded on an indigenous plant from a different family to the target weed. Further host specificity testing, including paired and multiple choice tests, are required to confirm the broad host range of P. brasiliensis. Other biological control agents that damage the woody stems of P. aculeata should be considered. The stem-borer, Acanthodoxus machacalis (Cerambycidae) is considered the most promising of the other candidate agents as it can be sourced from a climatically matched region where genetically suitable P. aculeata plants are found, it is sufficiently damaging to the woody stems of P. aculeata and there is no evidence that the species has a broad host range. Acanthodoxus machacalis should be sourced from Rio de Janeiro, Brazil, and imported into quarantine in South Africa for host specificity testing.
- Full Text:
- Date Issued: 2018
- Authors: Mdodana, Lumka Anita
- Date: 2018
- Subjects: Curculionidae -- South Africa , Cactus -- South Africa , Biological pest control agents , Alien plants-- South Africa , Pereskiophaga brasiliensis Anderson (Curculionidae) , Pereskia aculeata Miller (Cactaceae)
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/62350 , vital:28157
- Description: Pereskia aculeata Miller (Cactaceae) is a damaging invasive alien plant in South Africa that has negative impacts to indigenous biodiversity and ecosystem functioning. Mechanical and chemical control are not effective against P. aculeata so biological control is considered the only viable option. Two biological control agents, the leaf-feeding beetle Phenrica guerini Bechyne (Chrysomelidae) and the stem-wilting bug Catorhintha schaffneri (Coreidae), have been released in South Africa thus far. Post-release evaluations have indicated that P. guerini will not reduce P. aculeata densities to acceptable levels alone, while C. schaffneri was released very recently, so it is too soon to determine how effective that agent will be. Even if C. schaffneri is extremely damaging, it is likely that further agents will be required to reduce the densities of P. aculeata to acceptable levels within a reasonable time-scale. Additional agents should target the woody stems of P. aculeata which are not impacted by the damage of either of the released agents. Pereskiophaga brasiliensis Anderson (Curculionidae) is a promising potential candidate agent that feeds on the thick woody stems of the plant in the larval stage. Climatic matching, genetic matching and field based host specificity observations all indicated that P. brasiliensis was a promising candidate. In this study, the impact of P. brasiliensis to the target weed, P. aculeata, was quantified under quarantine conditions to determine whether it was sufficiently damaging to warrant release. This was followed by host specificity testing to determine whether P. brasiliensis was suitably host specific for release in South Africa. Impact studies indicated that P. brasiliensis was damaging to P. aculeata at insect densities that would be expected in the field. Pereskiophaga brasiliensis reduced the number of leaves of P. aculeata to a greater extent than it reduced shoot lengths, but both plant parameters were significantly reduced due to the feeding damage from the insect. This suggests that the damage from P. brasiliensis may be compatible with that of C. schaffneri which reduces shoot length to a greater degree than the number of leaves. Pereskiophaga brasiliensis is therefore sufficiently damaging to warrant release, and although interaction studies with the other agents would be required, it is expected that it should complement other existing agents. Although P. brasiliensis is sufficiently damaging, at present the host specificity data indicates that it is not suitably specific for release in South Africa because oviposition and larval development to the adult stage was recorded on both indigenous and alien plant species within the families Cactaceae and Basellaceae. This non-target feeding was recorded during no-choice tests, which are very conservative, but significant non-target damage and development to the adult stage was recorded on an indigenous plant from a different family to the target weed. Further host specificity testing, including paired and multiple choice tests, are required to confirm the broad host range of P. brasiliensis. Other biological control agents that damage the woody stems of P. aculeata should be considered. The stem-borer, Acanthodoxus machacalis (Cerambycidae) is considered the most promising of the other candidate agents as it can be sourced from a climatically matched region where genetically suitable P. aculeata plants are found, it is sufficiently damaging to the woody stems of P. aculeata and there is no evidence that the species has a broad host range. Acanthodoxus machacalis should be sourced from Rio de Janeiro, Brazil, and imported into quarantine in South Africa for host specificity testing.
- Full Text:
- Date Issued: 2018
Studies in leaf domatia-mite mutualism in South Africa
- Authors: Situngu, Sivuyisiwe
- Date: 2018
- Subjects: Insect-plant relationships , Mites , Mutualism (Biology) , Biological pest control agents
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/63334 , vital:28394
- Description: Plants have various traits which allow them to cope and resist their enemies including both insects and fungi . In some cases such traits allow plants to build mutualistic relationships with natural enemies of plant pests. This is the case in many dicotyledonous plants which produce leaf domatia. Leaf domatia are plant cavities usually found in the axils of major veins in the abaxial side of leaves. They are usually associated with mites and often mediate mutualistic relationships with predacious mites. Mites use leaf domatia primarily for shelter, to reproduce, and to develop. In turn, plants benefit from having predaceous mites on their leaves, because mites act as plant “bodyguards” and offer defence against pathogens and small arthropod herbivores. This phenomenon has been well documented all over the world, but Africa remains disproportionally understudied. The aim of this study was to fill the gap that exists in our knowledge of the extent of the distribution of leaf domatia-mite mutualisms and generate a better understanding of the diversity of mites found within leaf domatia from an African perspective. This was done by surveying plant species that bear leaf domatia from different vegetation types in South Africa. The plants with leaf domatia were examined for the presence of mites in order to determine patterns of mite abundance and diversity and, in so doing, address the following questions: • Does each tree species host have a specific mite or mite assemblage? • Do some mites prefer certain types of leaf domatia? • Do mites prefer a specific place in the tree canopy and does the microclimate in the tree canopy affect the distribution of mites? • Do different vegetation sites and types differ in their mite diversity and species composition? • Does mite abundance and diversity vary with seasons? Do coffee plantations have a different suite of mites than the adjacent forest? The anatomical structures of leaf domatia from six selected plant species(Coffea arabica, Gardenia thunbergia, Rothmannia capensis, Rothmannia globosa (Rubiaceae), Ocotea bullata (Lauraceae) and Tecoma capensis (Bignoniaceae) with different types of leaf domatia were also studied. The results from this study suggested that the key futures which distinguish domatia are the presence of an extra layer of tissue in the lower epidermis, a thick cuticle, cuticular folds, the presence of trichomes and an invagination. This study provides a better understating of the structure of leaf domatia. Leaf domatia bearing plants are widely distributed in South Africa, and species and vegetation-specific associations were assessed. Over 250 plant specimens with leaf domatia were collected and examined and more than 60 different mite species were found in association with the sampled plant species. The majority of mites found within the domatia of these tree species were predaceous and included mites from Stigmatidae, Tydeidae and Phytoseiidae. Furthermore, 15 new species were collected, suggesting that mites are understudied in South Africa. This study showed that the different vegetation types sampled did not differ markedly in terms of their mite biota and that similar mites were found across the region, and the association between leaf domatia and mites was found to be opportunistic and that mites had no preference for any particular domatia types. No host specificity relationship was observed between plants and mites. The assessment of mites associated with Coffea arabica showed that indigenous mites are able to colonise and establish a beneficial mutualism on exotic species. This is important as it ascertains that economically important plants that are cultivated outside their area of natural distribution can still benefit from this mutualism. This study also found that mite abundance and diversity in plants with leaf domatia were influenced by factors such as temperature, relative humidity and rainfall. Mite communities found in association with domatia changed as the year progressed and over the seasons. The seasonal fluctuations varied between the sampled plant species. In addition, this study found that mites were sensitive to extreme environmental conditions, and thus, mites preferred leaves found in the lower parts of the tree canopy and avoided exposed leaves. This study provides a better understanding of the distribution of domatia bearing plants in South Africa and their associated mites and contributes to our knowledge of the biodiversity of mites in the region. Furthermore, this study also adds to our understanding of the leaf domatia - mite mutualism in Africa. The applied example looking at the plant-mite mutualism in Coffea arabica highlights the importance of this mutualism in commercial plants.
- Full Text:
- Date Issued: 2018
- Authors: Situngu, Sivuyisiwe
- Date: 2018
- Subjects: Insect-plant relationships , Mites , Mutualism (Biology) , Biological pest control agents
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/63334 , vital:28394
- Description: Plants have various traits which allow them to cope and resist their enemies including both insects and fungi . In some cases such traits allow plants to build mutualistic relationships with natural enemies of plant pests. This is the case in many dicotyledonous plants which produce leaf domatia. Leaf domatia are plant cavities usually found in the axils of major veins in the abaxial side of leaves. They are usually associated with mites and often mediate mutualistic relationships with predacious mites. Mites use leaf domatia primarily for shelter, to reproduce, and to develop. In turn, plants benefit from having predaceous mites on their leaves, because mites act as plant “bodyguards” and offer defence against pathogens and small arthropod herbivores. This phenomenon has been well documented all over the world, but Africa remains disproportionally understudied. The aim of this study was to fill the gap that exists in our knowledge of the extent of the distribution of leaf domatia-mite mutualisms and generate a better understanding of the diversity of mites found within leaf domatia from an African perspective. This was done by surveying plant species that bear leaf domatia from different vegetation types in South Africa. The plants with leaf domatia were examined for the presence of mites in order to determine patterns of mite abundance and diversity and, in so doing, address the following questions: • Does each tree species host have a specific mite or mite assemblage? • Do some mites prefer certain types of leaf domatia? • Do mites prefer a specific place in the tree canopy and does the microclimate in the tree canopy affect the distribution of mites? • Do different vegetation sites and types differ in their mite diversity and species composition? • Does mite abundance and diversity vary with seasons? Do coffee plantations have a different suite of mites than the adjacent forest? The anatomical structures of leaf domatia from six selected plant species(Coffea arabica, Gardenia thunbergia, Rothmannia capensis, Rothmannia globosa (Rubiaceae), Ocotea bullata (Lauraceae) and Tecoma capensis (Bignoniaceae) with different types of leaf domatia were also studied. The results from this study suggested that the key futures which distinguish domatia are the presence of an extra layer of tissue in the lower epidermis, a thick cuticle, cuticular folds, the presence of trichomes and an invagination. This study provides a better understating of the structure of leaf domatia. Leaf domatia bearing plants are widely distributed in South Africa, and species and vegetation-specific associations were assessed. Over 250 plant specimens with leaf domatia were collected and examined and more than 60 different mite species were found in association with the sampled plant species. The majority of mites found within the domatia of these tree species were predaceous and included mites from Stigmatidae, Tydeidae and Phytoseiidae. Furthermore, 15 new species were collected, suggesting that mites are understudied in South Africa. This study showed that the different vegetation types sampled did not differ markedly in terms of their mite biota and that similar mites were found across the region, and the association between leaf domatia and mites was found to be opportunistic and that mites had no preference for any particular domatia types. No host specificity relationship was observed between plants and mites. The assessment of mites associated with Coffea arabica showed that indigenous mites are able to colonise and establish a beneficial mutualism on exotic species. This is important as it ascertains that economically important plants that are cultivated outside their area of natural distribution can still benefit from this mutualism. This study also found that mite abundance and diversity in plants with leaf domatia were influenced by factors such as temperature, relative humidity and rainfall. Mite communities found in association with domatia changed as the year progressed and over the seasons. The seasonal fluctuations varied between the sampled plant species. In addition, this study found that mites were sensitive to extreme environmental conditions, and thus, mites preferred leaves found in the lower parts of the tree canopy and avoided exposed leaves. This study provides a better understanding of the distribution of domatia bearing plants in South Africa and their associated mites and contributes to our knowledge of the biodiversity of mites in the region. Furthermore, this study also adds to our understanding of the leaf domatia - mite mutualism in Africa. The applied example looking at the plant-mite mutualism in Coffea arabica highlights the importance of this mutualism in commercial plants.
- Full Text:
- Date Issued: 2018
Yeast-baculovirus synergism: investigating mixed infections for improved management of the false codling moth, Thaumatotibia leucotreta
- Authors: Van der Merwe, Marcél
- Date: 2018
- Subjects: Cryptophlebia leucotreta , Baculoviruses , Yeast , Citrus Diseases and pests , Biological pest control agents , Pests Integrated control
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/62963 , vital:28347
- Description: Thaumatotibia leucotreta (Meyrick) (Lepidoptera: Tortricidae) or otherwise commonly known as the false codling moth is an indigenous pest of the citrus industry in southern Africa. The pest is highly significant as it impacts negatively on the export of fresh citrus fruits from South Africa to international markets. To control T. leucotreta in South Africa, an integrated pest management (IPM) programme has been implemented. One component of this programme is the baculovirus Cryptophlebia leucotreta granulovirus (CrleGV-SA) which has been formulated into the products Cryptogran™ and Cryptex®. It has previously been reported that there is a mutualistic association between Cydia pomonella (L.) (Lepidoptera: Tortricidae) also known as codling moth, and epiphytic yeasts. Cydia pomonella larval feeding galleries were colonised by yeasts and this, in turn, reduced larval mortality and enhanced larval development. It has been demonstrated in laboratory assays and field trials that combining yeast and brown cane sugar with Cydia pomonella granulovirus (CpGV) significantly increased larval mortality and lowered the proportion of injured apple fruit. This suggests that yeasts can enhance the effectiveness of an insect virus in managing pest larvae. In this study, we proposed to determine which species of yeast occur naturally in the digestive tract, frass and on the epidermis of T. leucotreta larvae and to examine whether any of these yeasts, when combined with the CrleGV-SA, have a synergistic effect in increasing mortality of T. leucotreta larvae. Firstly, Navel oranges infested with T. leucotreta larvae were collected from orchards in Sundays River Valley in Eastern Cape of South Africa. Larvae were extracted and analysed for the presence of yeast on their surface, or in their gut and frass. Four yeasts were isolated from T. leucotreta larvae and identified down to species level via PCR amplification and sequencing of internal transcribed spacer (ITS) region and D1/D2 domain of the large subunit (LSU) of rDNA region. These yeasts were isolated from the frass, epidermis and digestive tract of T. leucotreta larvae. The yeast isolates were identified as Meyerozyma caribbica, Pichia kluyveri, Pichia kudriavzevii and Hanseniaspora opuntiae. A yeast preference assay was conducted on female T. leucotreta moths to examine whether any of the isolated yeast species affected their oviposition preference. Navel oranges were inoculated with the isolated yeast species at a concentration of 6 × 108 cells.ml-1. The assay also included a Brewer’s yeast and distilled water control. Pichia kudriavzevii was shown to be the preferred yeast species for oviposition, as significantly more eggs were deposited on Navel oranges inoculated with this yeast compared to the other treatments. Lastly, a detached fruit bioassay was performed to evaluate the efficacy of mixing P. kudriavzevii with CrleGV-SA to enhance T. leucotreta larvae mortality. Pichia kudriavzevii was selected as it was demonstrated as having an effect on the oviposition preference of female T. leucotreta moths. The concentration at which P. kudriavzevii was applied remained the same as in the preference assay while CrleGV-SA was applied at lethal concentration required to kill 50 % of the population (9.31 × 107 OBs.ml-1). Although an increase in larval mortality was observed between CrleGV-SA being applied alone and the yeast/virus mixture, this result was determined not to be statistically significant. The experiments performed in this study provide a platform for further research into the application of a yeast-virus combination as a novel control option for T. leucotreta in the field. , Thesis (MSc) -- Faculty of Science, Biochemistry and Microbiology, 2018
- Full Text:
- Date Issued: 2018
- Authors: Van der Merwe, Marcél
- Date: 2018
- Subjects: Cryptophlebia leucotreta , Baculoviruses , Yeast , Citrus Diseases and pests , Biological pest control agents , Pests Integrated control
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/62963 , vital:28347
- Description: Thaumatotibia leucotreta (Meyrick) (Lepidoptera: Tortricidae) or otherwise commonly known as the false codling moth is an indigenous pest of the citrus industry in southern Africa. The pest is highly significant as it impacts negatively on the export of fresh citrus fruits from South Africa to international markets. To control T. leucotreta in South Africa, an integrated pest management (IPM) programme has been implemented. One component of this programme is the baculovirus Cryptophlebia leucotreta granulovirus (CrleGV-SA) which has been formulated into the products Cryptogran™ and Cryptex®. It has previously been reported that there is a mutualistic association between Cydia pomonella (L.) (Lepidoptera: Tortricidae) also known as codling moth, and epiphytic yeasts. Cydia pomonella larval feeding galleries were colonised by yeasts and this, in turn, reduced larval mortality and enhanced larval development. It has been demonstrated in laboratory assays and field trials that combining yeast and brown cane sugar with Cydia pomonella granulovirus (CpGV) significantly increased larval mortality and lowered the proportion of injured apple fruit. This suggests that yeasts can enhance the effectiveness of an insect virus in managing pest larvae. In this study, we proposed to determine which species of yeast occur naturally in the digestive tract, frass and on the epidermis of T. leucotreta larvae and to examine whether any of these yeasts, when combined with the CrleGV-SA, have a synergistic effect in increasing mortality of T. leucotreta larvae. Firstly, Navel oranges infested with T. leucotreta larvae were collected from orchards in Sundays River Valley in Eastern Cape of South Africa. Larvae were extracted and analysed for the presence of yeast on their surface, or in their gut and frass. Four yeasts were isolated from T. leucotreta larvae and identified down to species level via PCR amplification and sequencing of internal transcribed spacer (ITS) region and D1/D2 domain of the large subunit (LSU) of rDNA region. These yeasts were isolated from the frass, epidermis and digestive tract of T. leucotreta larvae. The yeast isolates were identified as Meyerozyma caribbica, Pichia kluyveri, Pichia kudriavzevii and Hanseniaspora opuntiae. A yeast preference assay was conducted on female T. leucotreta moths to examine whether any of the isolated yeast species affected their oviposition preference. Navel oranges were inoculated with the isolated yeast species at a concentration of 6 × 108 cells.ml-1. The assay also included a Brewer’s yeast and distilled water control. Pichia kudriavzevii was shown to be the preferred yeast species for oviposition, as significantly more eggs were deposited on Navel oranges inoculated with this yeast compared to the other treatments. Lastly, a detached fruit bioassay was performed to evaluate the efficacy of mixing P. kudriavzevii with CrleGV-SA to enhance T. leucotreta larvae mortality. Pichia kudriavzevii was selected as it was demonstrated as having an effect on the oviposition preference of female T. leucotreta moths. The concentration at which P. kudriavzevii was applied remained the same as in the preference assay while CrleGV-SA was applied at lethal concentration required to kill 50 % of the population (9.31 × 107 OBs.ml-1). Although an increase in larval mortality was observed between CrleGV-SA being applied alone and the yeast/virus mixture, this result was determined not to be statistically significant. The experiments performed in this study provide a platform for further research into the application of a yeast-virus combination as a novel control option for T. leucotreta in the field. , Thesis (MSc) -- Faculty of Science, Biochemistry and Microbiology, 2018
- Full Text:
- Date Issued: 2018
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