Effect of pomegranate (Punica granatum L) peel powder meal on growth performance, gut integrity, haemato-biochemical indices, meat, and bone quality of broiler chickens
- Akuru, Eunice Amaka https://orcid.org/0000-0002-3093-0247
- Authors: Akuru, Eunice Amaka https://orcid.org/0000-0002-3093-0247
- Date: 2022-04
- Subjects: Broilers (Chickens) , Meat -- Quality
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/23611 , vital:58218
- Description: The objective of the current study was to investigate the overall response of broiler birds to varying dietary levels of pomegranate (Punica granatum L) peel powder meal (PPPM). A total of 432-day-old Cobb 500 broiler chicks were randomly divided into six experimental groups of four replications with 18 birds per replicate in a completely randomized design (CRD). The birds were fed isonitrogenous and isocaloric experimental diets designated as: T1-control diet with 0percent additives (negative control; NEGCON); T2- control diet supplemented with α-tocopherol acetate at 200g per ton (positive control; POSCON): T3, T4, T5 and T6-control diet supplemented with 2, 4, 6 and 8g/kg PPPM (PPPM2, PPPM4, PPPM6, PPPM8). The phytochemical, nutritional and antioxidant properties of the pomegranate peel powder were determined. Results showed that the South African-grown pomegranate peel (“Wonderful” variety) had rich protein and trace mineral contents. Higher (p < 0.05) antioxidant and hepatic anti-lipid peroxidative activities were recorded in the ethanol and acetone extracts than the water extract, whereas the three extracts had no toxic effects on liver and kidney cells (p > 0.05). The dietary effects of PPPM supplementation on growth performance, digestibility, carcass, and organ weight indices of Cobb 500 birds were determined. The POSCON diet enhanced (p < 0.05) feed conversion ratio (FCR) at week 3. The average final body weight and average daily weight gain were highest (p < 0.05) in birds fed PPPM2 and PPPM4 diets, whereas birds fed PPPM2 diet had enhanced (p < 0.05) FCR and protein efficiency ratio when compared with birds on POSCON. Thigh and breast weights were highest (p < 0.05) in birds on PPPM4 and PPPM8 diets, whereas birds fed PPPM4 diet had enhanced (p < 0.05) nutrient digestibility compared with those on POSCON. The antioxidant status and breast meat quality of Cobb 500 broiler birds fed diets supplemented with PPPM were determined. Meat from birds fed PPPM8 diet had the highest (p < 0.05) thawing loss, while cooking loss was lowest (p < 0.05) in the PPPM2 group. The highest (p < 0.05) ability to scavenge ABTS [(2, 2-azinobis (3ethylbenzothiazoline-6 sulfonic acid))] radical cation (ABTS+) was recorded in the PPPM2 and PPPM4 meat while PPPM8 meat had increased (p < 0.05) catalase activity. The fatty acid composition, oxidative status, and drip loss in breast meat of Cobb 500 broiler birds fed graded levels of PPPM was determined. Meat from birds fed PPPM4 diet had the lowest (p < 0.05) margaric (C17:0) and arachidic (C20:0) acids, while meat from birds fed PPPM8 diet had higher (p < 0.05) levels of eicosadenoic (C20:2, n-6) and arachidonic (AA, 20:4) acids. The POSCON had higher (p < 0.05) ability to reduce TBARS levels in meat on day-1 and day-16 of storage than the NEGCON and PPPM diets, whereas TBARS levels were lowest (p < 0.05) in PPPM6 meat on day-16. The gut pH, histology, haematology, serum-biochemical indices, and bone quality parameters of Cobb 500 broiler birds fed diets supplemented with PPPM was determined. Birds fed POSCON, PPM2 and PPPM4 diets had the lowest (p < 0.05) pH in the duodenum, jejunum, and caecum, whereas duodenal pH values were lowest (p < 0.05) in birds fed PPPM2 and PPPM4 diets compared with birds on NEGCON. Birds fed PPPM4 diet had the highest (p < 0.01) duodenal, jejunal and ileal villi height (VH), and the highest (p < 0.01) VH to crypt depth (CD) ratio in the duodenum, jejunum, and ileum. The lowest (p < 0.05) CD in the duodenum, jejunum and ileum was also recorded in birds fed PPPM4 diet. Birds fed PPPM8 diet had higher (p < 0.05) values for white blood cell count, red blood cell count, haemoglobin concentration and packed cell volume, whereas the lowest (p < 0.05) concentration of serum aspartate transaminase was recorded in birds fed PPPM4 diet. Birds on PPM4 diet had longer (p < 0.05) bones. Bone weight and bone breaking strength were highest (p < 0.05) in birds fed PPPM8 diet. Tibia zinc and iron concentrations increased as the inclusion levels of pomegranate peel powder meal increased. It was concluded that broiler birds require 4 g/kg pomegranate peel powder for enhanced growth performance, nutrient digestibility, gut histology, quality, and antioxidant enzyme activity of meat, whereas 8 g/kg pomegranate peel powder meal supplementation is required for enhanced heamato-biochemical indices, bone quality, fatty acid composition and lipid peroxidative parameters in broiler birds. , Thesis (PhD) -- Faculty of Science and Agriculture, 2022
- Full Text:
- Date Issued: 2022-04
- Authors: Akuru, Eunice Amaka https://orcid.org/0000-0002-3093-0247
- Date: 2022-04
- Subjects: Broilers (Chickens) , Meat -- Quality
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/23611 , vital:58218
- Description: The objective of the current study was to investigate the overall response of broiler birds to varying dietary levels of pomegranate (Punica granatum L) peel powder meal (PPPM). A total of 432-day-old Cobb 500 broiler chicks were randomly divided into six experimental groups of four replications with 18 birds per replicate in a completely randomized design (CRD). The birds were fed isonitrogenous and isocaloric experimental diets designated as: T1-control diet with 0percent additives (negative control; NEGCON); T2- control diet supplemented with α-tocopherol acetate at 200g per ton (positive control; POSCON): T3, T4, T5 and T6-control diet supplemented with 2, 4, 6 and 8g/kg PPPM (PPPM2, PPPM4, PPPM6, PPPM8). The phytochemical, nutritional and antioxidant properties of the pomegranate peel powder were determined. Results showed that the South African-grown pomegranate peel (“Wonderful” variety) had rich protein and trace mineral contents. Higher (p < 0.05) antioxidant and hepatic anti-lipid peroxidative activities were recorded in the ethanol and acetone extracts than the water extract, whereas the three extracts had no toxic effects on liver and kidney cells (p > 0.05). The dietary effects of PPPM supplementation on growth performance, digestibility, carcass, and organ weight indices of Cobb 500 birds were determined. The POSCON diet enhanced (p < 0.05) feed conversion ratio (FCR) at week 3. The average final body weight and average daily weight gain were highest (p < 0.05) in birds fed PPPM2 and PPPM4 diets, whereas birds fed PPPM2 diet had enhanced (p < 0.05) FCR and protein efficiency ratio when compared with birds on POSCON. Thigh and breast weights were highest (p < 0.05) in birds on PPPM4 and PPPM8 diets, whereas birds fed PPPM4 diet had enhanced (p < 0.05) nutrient digestibility compared with those on POSCON. The antioxidant status and breast meat quality of Cobb 500 broiler birds fed diets supplemented with PPPM were determined. Meat from birds fed PPPM8 diet had the highest (p < 0.05) thawing loss, while cooking loss was lowest (p < 0.05) in the PPPM2 group. The highest (p < 0.05) ability to scavenge ABTS [(2, 2-azinobis (3ethylbenzothiazoline-6 sulfonic acid))] radical cation (ABTS+) was recorded in the PPPM2 and PPPM4 meat while PPPM8 meat had increased (p < 0.05) catalase activity. The fatty acid composition, oxidative status, and drip loss in breast meat of Cobb 500 broiler birds fed graded levels of PPPM was determined. Meat from birds fed PPPM4 diet had the lowest (p < 0.05) margaric (C17:0) and arachidic (C20:0) acids, while meat from birds fed PPPM8 diet had higher (p < 0.05) levels of eicosadenoic (C20:2, n-6) and arachidonic (AA, 20:4) acids. The POSCON had higher (p < 0.05) ability to reduce TBARS levels in meat on day-1 and day-16 of storage than the NEGCON and PPPM diets, whereas TBARS levels were lowest (p < 0.05) in PPPM6 meat on day-16. The gut pH, histology, haematology, serum-biochemical indices, and bone quality parameters of Cobb 500 broiler birds fed diets supplemented with PPPM was determined. Birds fed POSCON, PPM2 and PPPM4 diets had the lowest (p < 0.05) pH in the duodenum, jejunum, and caecum, whereas duodenal pH values were lowest (p < 0.05) in birds fed PPPM2 and PPPM4 diets compared with birds on NEGCON. Birds fed PPPM4 diet had the highest (p < 0.01) duodenal, jejunal and ileal villi height (VH), and the highest (p < 0.01) VH to crypt depth (CD) ratio in the duodenum, jejunum, and ileum. The lowest (p < 0.05) CD in the duodenum, jejunum and ileum was also recorded in birds fed PPPM4 diet. Birds fed PPPM8 diet had higher (p < 0.05) values for white blood cell count, red blood cell count, haemoglobin concentration and packed cell volume, whereas the lowest (p < 0.05) concentration of serum aspartate transaminase was recorded in birds fed PPPM4 diet. Birds on PPM4 diet had longer (p < 0.05) bones. Bone weight and bone breaking strength were highest (p < 0.05) in birds fed PPPM8 diet. Tibia zinc and iron concentrations increased as the inclusion levels of pomegranate peel powder meal increased. It was concluded that broiler birds require 4 g/kg pomegranate peel powder for enhanced growth performance, nutrient digestibility, gut histology, quality, and antioxidant enzyme activity of meat, whereas 8 g/kg pomegranate peel powder meal supplementation is required for enhanced heamato-biochemical indices, bone quality, fatty acid composition and lipid peroxidative parameters in broiler birds. , Thesis (PhD) -- Faculty of Science and Agriculture, 2022
- Full Text:
- Date Issued: 2022-04
Effects of feeding Moringa Oleifera leaf meal as an additive on growth performance of chicken, physico-chemical shelf-life indicators, fatty acid profiles and lipid oxidation of broiler meat
- Authors: Wapi, Cwayita
- Date: 2012
- Subjects: Moringa , Broilers (Chickens) , Meat -- Quality
- Language: English
- Type: Thesis , Masters , MSc Agric (Animal Science)
- Identifier: vital:11822 , http://hdl.handle.net/10353/d1007546 , Moringa , Broilers (Chickens) , Meat -- Quality
- Description: Effects of feeding Moringa oleifera leaf meal as an additive on growth performance of chicken, physico- chemical shelf-life indicators, fatty acids profiles and lipid oxidation of broiler meat The main objective of the study was to determine the effect of M.oleifera leaf meal (MOLM) as an additive on growth performance, carcass characteristics, physico-chemical shelf-life indicators (colour, ultimate pH, driploss), fatty acids profiles and lipid oxidation of meat from broilers. A total of 432 1day old unsexed broiler chicks (Aviane 48) were randomly allocated to four dietary treatments (TRTS) in 72 cages. There were 18 cages per treatment and each cage allocated 6 chicks. Water and feed was provided at ad libitum. The feeding phases were, prestarter (0-7 Days), starter (8-18 Days), grower (19-28 Days), finisher (29-35 Days). The four TRTS contained graded levels of MOLM at 1000g/ton, 750g/ton, 500g/ton, and 0g/ton (control), respectively. The birds were slaughtered at 35 days of age. Breast muscles were sampled for meat, ultimate pH (pHu ), colour, drip loss over a 7 days shelf-life test. After each day’s test sub-samples were dipped in liquid nitrogen and kept at -180 C for thiobarbituric acid reactive substances determination. On Day1 and Day 7 extra sub-samples were also kept at -180 C for fatty acids analysis.The TRTS had no effect on average feed intake (AFI), feed conversion efficiency (FCE), and on average daily gain (ADG). Slaughter weight (SW), carcass weight (CW), dressing percentage and gizzard weight (GW) values were similar in all TRTS. Liver weight (LW), heart weight (HW), and gastro-intestinal fat (GIF) differed in all the TRTS, with treatment 2 having the highest value of HW (28.3±2.55), and LW (44.2±1.60) was the highest on treatment 4 . The pH values in all TRTS were constant from Day1 to Day5, reached peak on Day6, and then declined on Day7. Meat from broilers given treatment 1 with MOLM (1000g/ton) had the highest lightness (L*) values. The redness (a*) values were the highest in meat from treatment 2 (750g/ton MOLM). Treatments had no effect on yellowness (b*) values and on drip loss of the breasts. During storage L* values were high from Day1 to Day5 and decreased from Day6 to Day7. Drip loss increased with storage time as expected. Treatment 4 (control) had the highest proportions of poly-unsaturated fatty acids (PUFA) (30.3±1.87). Treatment 1 (1000g/ton) had the highest proportion of saturated fatty acids (SFA) (60.9±1.87). Treatment 1 (1000g/ton) had the highest proportion of SFA (60.9±4.30). Treatment 2 (750g/ton) had the highest n-6/n-3 ratio than other TRTS. Days had no effect (P>0.05) on PUFA, SFA, and n-6/n-3 ratio. Treatment 1 had a highest amount of malondialdehyde (MDA), treatment 4 had no effect (P>0.05) on MDA . Storage time had an effect (P<0.05) on MDA levels, except for on Day1 and Day7. Day2 had the highest amount of MDA (0.7±0.08). The use of MOLM as an additive in broiler diets reduced lipid oxidation in meat, and maintained the quality of the broiler meat during storage. It also did not have any adverse effects on the growth performance of broilers. Therefore, it has the potential to be used as an additive in broiler diets.
- Full Text:
- Date Issued: 2012
- Authors: Wapi, Cwayita
- Date: 2012
- Subjects: Moringa , Broilers (Chickens) , Meat -- Quality
- Language: English
- Type: Thesis , Masters , MSc Agric (Animal Science)
- Identifier: vital:11822 , http://hdl.handle.net/10353/d1007546 , Moringa , Broilers (Chickens) , Meat -- Quality
- Description: Effects of feeding Moringa oleifera leaf meal as an additive on growth performance of chicken, physico- chemical shelf-life indicators, fatty acids profiles and lipid oxidation of broiler meat The main objective of the study was to determine the effect of M.oleifera leaf meal (MOLM) as an additive on growth performance, carcass characteristics, physico-chemical shelf-life indicators (colour, ultimate pH, driploss), fatty acids profiles and lipid oxidation of meat from broilers. A total of 432 1day old unsexed broiler chicks (Aviane 48) were randomly allocated to four dietary treatments (TRTS) in 72 cages. There were 18 cages per treatment and each cage allocated 6 chicks. Water and feed was provided at ad libitum. The feeding phases were, prestarter (0-7 Days), starter (8-18 Days), grower (19-28 Days), finisher (29-35 Days). The four TRTS contained graded levels of MOLM at 1000g/ton, 750g/ton, 500g/ton, and 0g/ton (control), respectively. The birds were slaughtered at 35 days of age. Breast muscles were sampled for meat, ultimate pH (pHu ), colour, drip loss over a 7 days shelf-life test. After each day’s test sub-samples were dipped in liquid nitrogen and kept at -180 C for thiobarbituric acid reactive substances determination. On Day1 and Day 7 extra sub-samples were also kept at -180 C for fatty acids analysis.The TRTS had no effect on average feed intake (AFI), feed conversion efficiency (FCE), and on average daily gain (ADG). Slaughter weight (SW), carcass weight (CW), dressing percentage and gizzard weight (GW) values were similar in all TRTS. Liver weight (LW), heart weight (HW), and gastro-intestinal fat (GIF) differed in all the TRTS, with treatment 2 having the highest value of HW (28.3±2.55), and LW (44.2±1.60) was the highest on treatment 4 . The pH values in all TRTS were constant from Day1 to Day5, reached peak on Day6, and then declined on Day7. Meat from broilers given treatment 1 with MOLM (1000g/ton) had the highest lightness (L*) values. The redness (a*) values were the highest in meat from treatment 2 (750g/ton MOLM). Treatments had no effect on yellowness (b*) values and on drip loss of the breasts. During storage L* values were high from Day1 to Day5 and decreased from Day6 to Day7. Drip loss increased with storage time as expected. Treatment 4 (control) had the highest proportions of poly-unsaturated fatty acids (PUFA) (30.3±1.87). Treatment 1 (1000g/ton) had the highest proportion of saturated fatty acids (SFA) (60.9±1.87). Treatment 1 (1000g/ton) had the highest proportion of SFA (60.9±4.30). Treatment 2 (750g/ton) had the highest n-6/n-3 ratio than other TRTS. Days had no effect (P>0.05) on PUFA, SFA, and n-6/n-3 ratio. Treatment 1 had a highest amount of malondialdehyde (MDA), treatment 4 had no effect (P>0.05) on MDA . Storage time had an effect (P<0.05) on MDA levels, except for on Day1 and Day7. Day2 had the highest amount of MDA (0.7±0.08). The use of MOLM as an additive in broiler diets reduced lipid oxidation in meat, and maintained the quality of the broiler meat during storage. It also did not have any adverse effects on the growth performance of broilers. Therefore, it has the potential to be used as an additive in broiler diets.
- Full Text:
- Date Issued: 2012
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