Effect of organisational policies on school management teams' job performance at Maluti District, Eastern Cape , South Africa
- Authors: Leboea, Paulos Dipholo
- Date: 2016
- Subjects: School management teams School management and organization -- South Africa -- Eastern Cape Total quality management in higher education -- South Africa -- Eastern Cape Performance -- Management
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10353/2293 , vital:27748
- Description: Most school management teams (SMTs) do not do their tasks either due to lack of time or inability to fulfil this obligation. Should this be true for South African schools, it would reflect negatively on the SMT job performance. This led to undertaking research in order to ascertain the fit between organisational policies of induction, mentoring, curriculum assessment training and curriculum supervision and the SMT job performance by way of establishing the effect of organisation policy on SMTs’ job performance. The research adopted a descriptive survey research design of the correlational type. Two sets of questionnaires, one for independent variables and the other for the dependent variable, were used to elicit information from the respondents. Some of the findings show that aspiring SMT members need some training so as to know what will be expected of them to do once they get selected as SMT members. It was further proven that common tests that are normally set at the provincial and district and/or school based levels are invariably not of the standard that the Department of Basic Education (DBE) prescribes nationally. The foregoing led to the conclusion that the SMT job practice is invariably not informed by the relevant organisational policies. Hence a cascade model of SMT training is recommended, as is the notion that training toward organisational policies under review should be done cohesively.
- Full Text:
- Authors: Leboea, Paulos Dipholo
- Date: 2016
- Subjects: School management teams School management and organization -- South Africa -- Eastern Cape Total quality management in higher education -- South Africa -- Eastern Cape Performance -- Management
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10353/2293 , vital:27748
- Description: Most school management teams (SMTs) do not do their tasks either due to lack of time or inability to fulfil this obligation. Should this be true for South African schools, it would reflect negatively on the SMT job performance. This led to undertaking research in order to ascertain the fit between organisational policies of induction, mentoring, curriculum assessment training and curriculum supervision and the SMT job performance by way of establishing the effect of organisation policy on SMTs’ job performance. The research adopted a descriptive survey research design of the correlational type. Two sets of questionnaires, one for independent variables and the other for the dependent variable, were used to elicit information from the respondents. Some of the findings show that aspiring SMT members need some training so as to know what will be expected of them to do once they get selected as SMT members. It was further proven that common tests that are normally set at the provincial and district and/or school based levels are invariably not of the standard that the Department of Basic Education (DBE) prescribes nationally. The foregoing led to the conclusion that the SMT job practice is invariably not informed by the relevant organisational policies. Hence a cascade model of SMT training is recommended, as is the notion that training toward organisational policies under review should be done cohesively.
- Full Text:
Effect of phosphorus application on dry matter yield and nutrient content of Mucuna Pruriens (L) in Alice
- Authors: Tshwati, Nolubabalo
- Date: 2016
- Subjects: Legumes Phosphorus Fertilizers
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/12326 , vital:39253
- Description: The use of herbaceous legumes such as Mucuna (Mucuna pruriens) can assist to recapitalize soil fertility depletion and improve livestock productivity in the small holder farming sector of South Africa. The objective of the study was to investigate the effect of phosphorus fertilizer application (0, 20, 40 and 60 kg P/ha) on the dry matter yield and nutritive value of Mucuna pruriens. The study was conducted at University of Fort Hare Research farm in Alice, South Africa. The research design was a randomized complete block design with three replicates per treatment. Data collection included biomass production and nutrient content of Mucuna pruriens. The forage legume was harvested at flowering stage of growth and analysed for chemical composition. The results showed that with the application of 60 kg P/ha Mucuna had the highest (P < 0.05) fresh and dry matter yield of 19.58 kg/ha and 5.41 kg/ha, respectively compared to other treatments. However, all the other treatments differed from one another. Similarly, the fresh stem and leaf fractions yield and their dry matter yields showed the same trend with application of 60 kg P/ha having the greatest yield compared to the other treatments. Nevertheless, all the treatments differed (P<0.05) from one another with P0 having the least yields. The leaf to stem ratio did not show any significant (P>0.05) difference among all the treatments ranging from 1.44 to 1.62 in fresh forage and ranging from 1.31 to 1.50 in the dry forage. In terms of dry matter content, the control treatment had the highest dry matter content of 37.78percent, however, all the other treatments did not differ significantly (P < 0.05) from each other. There was no significant effect of P application (P > 0.05) in terms of ash, EE, ADF, CP, Ca, Mg, K, Na, P, Zn, Cu, Mn and Fe of the whole plant forage among the treatments. The application of 40 kg P/ha fertilizer significantly (P < 0.05) increased the NDF (51.16percent) of the whole plant of Mucuna compared to the other treatments which did not differ (p>0.05) among themselves. Within the stem fraction there were no significant (P > 0.05) differences among the treatments for the ash, EE, ADF, NDF, and CP, hemicellulose, Ca, Mg, K, P, Zn, Cu, Mn and Fe contents. However, the application of 40 kg P/ha fertilizer significantly (P < 0.05) increased the Na (0.05percent) content of the Mucuna stem fraction. In the leaf fraction of Mucuna there was no significant (P > 0.05) differences in terms of ash, EE, ADF, NDF, CP, hemicellulose, Ca, K, P, Zn, Cu, Mn and Fe among the treatments. However, the application of 20 kg P/ha fertilizer significantly (P < 0.05) increased the Mg (0.52percent) of the Mucuna leaves compared to the other treatments. Therefore the study has shown that increase in P application (60 kg P/ha) has no major effect in the nutrient content of Mucuna. Furthermore, it is recommended that Mucuna can be grown as a protein supplement, based on the CP content values that were recorded in this study and to provide maintenance requirements for ruminants. It was therefore concluded that phosphorus fertilization particularly 60 kg P/ha is necessary and required for optimum growth of Mucuna pruriens in order to increase forage yield. Based on the nutrient content result it was concluded that there was no effect of P fertilizer application levels on the nutrient content of Mucuna pruriens in Alice.
- Full Text:
- Authors: Tshwati, Nolubabalo
- Date: 2016
- Subjects: Legumes Phosphorus Fertilizers
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/12326 , vital:39253
- Description: The use of herbaceous legumes such as Mucuna (Mucuna pruriens) can assist to recapitalize soil fertility depletion and improve livestock productivity in the small holder farming sector of South Africa. The objective of the study was to investigate the effect of phosphorus fertilizer application (0, 20, 40 and 60 kg P/ha) on the dry matter yield and nutritive value of Mucuna pruriens. The study was conducted at University of Fort Hare Research farm in Alice, South Africa. The research design was a randomized complete block design with three replicates per treatment. Data collection included biomass production and nutrient content of Mucuna pruriens. The forage legume was harvested at flowering stage of growth and analysed for chemical composition. The results showed that with the application of 60 kg P/ha Mucuna had the highest (P < 0.05) fresh and dry matter yield of 19.58 kg/ha and 5.41 kg/ha, respectively compared to other treatments. However, all the other treatments differed from one another. Similarly, the fresh stem and leaf fractions yield and their dry matter yields showed the same trend with application of 60 kg P/ha having the greatest yield compared to the other treatments. Nevertheless, all the treatments differed (P<0.05) from one another with P0 having the least yields. The leaf to stem ratio did not show any significant (P>0.05) difference among all the treatments ranging from 1.44 to 1.62 in fresh forage and ranging from 1.31 to 1.50 in the dry forage. In terms of dry matter content, the control treatment had the highest dry matter content of 37.78percent, however, all the other treatments did not differ significantly (P < 0.05) from each other. There was no significant effect of P application (P > 0.05) in terms of ash, EE, ADF, CP, Ca, Mg, K, Na, P, Zn, Cu, Mn and Fe of the whole plant forage among the treatments. The application of 40 kg P/ha fertilizer significantly (P < 0.05) increased the NDF (51.16percent) of the whole plant of Mucuna compared to the other treatments which did not differ (p>0.05) among themselves. Within the stem fraction there were no significant (P > 0.05) differences among the treatments for the ash, EE, ADF, NDF, and CP, hemicellulose, Ca, Mg, K, P, Zn, Cu, Mn and Fe contents. However, the application of 40 kg P/ha fertilizer significantly (P < 0.05) increased the Na (0.05percent) content of the Mucuna stem fraction. In the leaf fraction of Mucuna there was no significant (P > 0.05) differences in terms of ash, EE, ADF, NDF, CP, hemicellulose, Ca, K, P, Zn, Cu, Mn and Fe among the treatments. However, the application of 20 kg P/ha fertilizer significantly (P < 0.05) increased the Mg (0.52percent) of the Mucuna leaves compared to the other treatments. Therefore the study has shown that increase in P application (60 kg P/ha) has no major effect in the nutrient content of Mucuna. Furthermore, it is recommended that Mucuna can be grown as a protein supplement, based on the CP content values that were recorded in this study and to provide maintenance requirements for ruminants. It was therefore concluded that phosphorus fertilization particularly 60 kg P/ha is necessary and required for optimum growth of Mucuna pruriens in order to increase forage yield. Based on the nutrient content result it was concluded that there was no effect of P fertilizer application levels on the nutrient content of Mucuna pruriens in Alice.
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Effects of frequent changes in curriculum on effective teaching and learning for creating safe, caring and child-friendly schools : a South African case study
- Authors: Makwarela, Mawela Convince
- Date: 2016
- Subjects: School discipline--South Africa School violence--South Africa School management and organization--South Africa
- Language: English
- Type: Thesis , Doctoral , Education
- Identifier: http://hdl.handle.net/10353/15594 , vital:40481
- Description: South Africa‟s government strategy for Child Protection Programmes is the pre-requisite that plans to articulate its work on school learner protection as a distinct area of programmatic intervention. Hence, this study explores an assessment of the implementation of DoE and UNICEF guidelines for creating safe, caring and child-friendly schools. A sequential mixed method approach was used in this study; both questionnaires and interviews were used. The design allows using both quantitative and qualitative data collection methods to elicit implicit and explicit information from the same case through pattern-matching; a technique linking several pieces when a holistic and in-depth investigation is required. The sample of the study consisted of 24 school principals, 24 HoDs, 24 SGB members, 24 educators, 24 educators‟ union members and 24 learners who were conveniently selected from 24 secondary schools across Vhembe District. Survey data were obtained from school principals, HoDs, SGB members, educators, educators‟ union members and learners regarding the ways and degree to which participants are involved in school incidents, decision-making processes, and their own experiences regarding safe, caring and child-friendly schools. In terms of data analysis, validation indicates concurrent and construct validity obtained by combining participant‟s responses from interviews and self-report questionnaires based on experiences of incidents from their schools. Statistically significant results point the way to validating the approaches taken as best practices. The findings reveals among others that the factors contributing to learner aggression include family factors, environmental factors and school-related factors, whilst the most common forms of learner incidents in schools are verbal violence, physical violence and bullying. The results also indicate that a safe school climate is not enough for learners to speak freely and report unwanted sexual experiences. The study concludes with the role that the school, parents and the Department of Education can play in addressing learner problems in schools.
- Full Text:
- Authors: Makwarela, Mawela Convince
- Date: 2016
- Subjects: School discipline--South Africa School violence--South Africa School management and organization--South Africa
- Language: English
- Type: Thesis , Doctoral , Education
- Identifier: http://hdl.handle.net/10353/15594 , vital:40481
- Description: South Africa‟s government strategy for Child Protection Programmes is the pre-requisite that plans to articulate its work on school learner protection as a distinct area of programmatic intervention. Hence, this study explores an assessment of the implementation of DoE and UNICEF guidelines for creating safe, caring and child-friendly schools. A sequential mixed method approach was used in this study; both questionnaires and interviews were used. The design allows using both quantitative and qualitative data collection methods to elicit implicit and explicit information from the same case through pattern-matching; a technique linking several pieces when a holistic and in-depth investigation is required. The sample of the study consisted of 24 school principals, 24 HoDs, 24 SGB members, 24 educators, 24 educators‟ union members and 24 learners who were conveniently selected from 24 secondary schools across Vhembe District. Survey data were obtained from school principals, HoDs, SGB members, educators, educators‟ union members and learners regarding the ways and degree to which participants are involved in school incidents, decision-making processes, and their own experiences regarding safe, caring and child-friendly schools. In terms of data analysis, validation indicates concurrent and construct validity obtained by combining participant‟s responses from interviews and self-report questionnaires based on experiences of incidents from their schools. Statistically significant results point the way to validating the approaches taken as best practices. The findings reveals among others that the factors contributing to learner aggression include family factors, environmental factors and school-related factors, whilst the most common forms of learner incidents in schools are verbal violence, physical violence and bullying. The results also indicate that a safe school climate is not enough for learners to speak freely and report unwanted sexual experiences. The study concludes with the role that the school, parents and the Department of Education can play in addressing learner problems in schools.
- Full Text:
Effects of grazing and housing management systems on greenhouse gas emissions, blood profiles and meat quality of Nguni goats
- Authors: Tapiwa, Chikwanda Allen
- Date: 2016
- Subjects: Goats Goats -- Feeding and feeds Greenhouse gas mitigation , Grazing -- Environmental aspects
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10353/2314 , vital:27752
- Description: The broad objective of this study was to evaluate the effects of grazing and housing management systems on greenhouse gas emissions, blood profiles and meat quality of Nguni goats. A total of 64 castrated Nguni goats were used and a housing structure was designed to accommodate the goats as well as evaluate effects of type of floor on greenhouse gases, meat quality, blood metabolites and fatty acid profiles. In the first experiment: Forty-eight castrated Nguni goats, at 6-7 months old were used for herding (H) or tethering (T) management systems and randomly allocated to pens with earth (E) or slatted (S) floors. Gas sampling was done using static chambers and analysed using a SRI 8610C gas chromatography machine. The highest CH4 fluxes were from the manure of herded goats on earth floors (HE). The highest CO2 fluxes were from manure of herded goats that were housed on slatted floors (HS): The highest N2O fluxes were from the manure of tethered goats that were sheltered on slatted floors (TS). It was concluded from the first experiment that goat manure is an important source of GHG that is influenced by grazing and floor type management systems. The second experiment was done to evaluate greenhouse gas (GHG) emission from disused (DP) vs occupied goat pens (OP) with earth (EF) or slatted (SF) floors. Eight previously occupied pens were used in this study. Sixteen goats were randomly put in the pens labelled occupied pens with earth floors (OPEF) and occupied pens with slatted floors (OPSF) resulting in four goats per OP. There were month by pen use; month by floor type; and use by floor type interaction effects (P < 0.05) on CH4 production. Floor type, use (DP vs OP) and month affected (P < 0.05) CH4 production. Manure on earth floors of occupied pens (OPEF) emitted the highest (P < 0.05) methane followed by earth floors of disused pens (DPEF). The least CH4 was emitted from DPSF. There was pen use (DP vs OP) by floor type interaction effect (P < 0.05) on CO2 emission. Floor type and pen use affected (P < 0.05) CO2 production. Manure in occupied pens with slatted floors (OPSF) produced the highest CO2 and the least CO2 was from disused pens with slatted floors (DPSF). Floor type and month influenced (P < 0.05) N2O production. It was concluded that continued use of goat pens increased GHG emissions. However, disused goat pens with manure remain an important source of GHG as they continued to emit quantifiable levels of CH4, CO2 and N2O. The third experiment was done to determine the effects of grazing and housing management systems on the concentrations of blood metabolites, activity of creatine kinase, body weight (BW), body condition score (BCS) and carcass characteristics using 48 castrated Nguni goats. Tethered goats had higher (P<0.05): urea, creatinine, total protein, globulin concentrations and more activity of CK than herded goats. On the other hand, herded goats had higher (P<0.05) plasma glucose (H: 3.39±0.020 vs T: 3.23±0.020 mmol/L), serum albumin (H: 15.30±0.147 vs T: 14.82±0.144 g/L) concentration. It was concluded that serum concentrations of all the metabolites and the activity of CK were sensitive to grazing system. Herding of goats promoted more glucose concentration and preservation of muscle protein and hence superior body weights and BCS than tethered goats. The fourth experiment was done to determine the effects of grazing and housing management systems on the activity of creatine kinase (CK) and cortisol concentration, and chevon quality. Further, the effect of muscle type [Muscularis longissimus thoracis et. Lumborum (LTL) and triceps brachii (TB)] on chevon quality was also investigated. Forty eight castrated Nguni goats aged 6- 7 months were randomly allocated to tethering (n=24) and herding (n=24) treatments. Floor type affected meat (P<0.05) pH24. Tethered goats had higher (P < 0.05) L* and CL percent than herded goats. Muscle type had a significant influence (P < 0.05) on a*, WBSF and pH. It was observed and concluded that T resulted in higher L* and more CL percent whilst SF lowered the pH24 of chevon; at the same time meat from the TB muscle was more tender, despite having a higher pH24 and more CL percent relative to LTH cuts. The fifth experiment was done to determine the effect of grazing and floor type on fatty acid composition of chevon from tethered and herded Nguni goats. Floor type had no effect on fatty acid composition. Oleic, stearic, palmitic and linoleic were the major fatty acids. Chevon from tethered goats had higher (P < 0.05) proportions of long chain polyunsaturated fatty acids (PUFAs): eicosapentaenoic, docosapentaenoic, docosahexaenoic, conjugated linoleic acid (CLA) and more total SFA, total PUFA, total Omega- 6 and total omega 3 fatty acids than herded goats. On the other hand, chevon from herded goats had more (P < 0.05) C18:1c9, C18:2t9, 12 (n-6), C20:3c11, 14, 17 (n-3) and total MUFA than tethered goats. However, there was no difference (P > 0.05) in the PUFA: SFA and n-6/n-3 fatty acid ratios between the herded and tethered goats. It was concluded that tethering promoted higher proportions of beneficial PUFAs which were more in TB than LTH muscle. The overall conclusions from the whole study were that goat manure is an important source of GHG with high CH4 being emitted from earth floors of herded goats. High CO2 and N2O are emitted from SF of herded and tethered goats, respectively. There were no major differences in meat quality between herded and tethered goats. However, herding promoted more live weight gain than tethering. On the other hand, tethering promoted more beneficial fatty acids. Therefore depending on the objective of the enterprise, smallholder farmers can safely tether or herd their goats. Further, there is need to develop technologies to harness CH4 from goat manure.
- Full Text:
- Authors: Tapiwa, Chikwanda Allen
- Date: 2016
- Subjects: Goats Goats -- Feeding and feeds Greenhouse gas mitigation , Grazing -- Environmental aspects
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10353/2314 , vital:27752
- Description: The broad objective of this study was to evaluate the effects of grazing and housing management systems on greenhouse gas emissions, blood profiles and meat quality of Nguni goats. A total of 64 castrated Nguni goats were used and a housing structure was designed to accommodate the goats as well as evaluate effects of type of floor on greenhouse gases, meat quality, blood metabolites and fatty acid profiles. In the first experiment: Forty-eight castrated Nguni goats, at 6-7 months old were used for herding (H) or tethering (T) management systems and randomly allocated to pens with earth (E) or slatted (S) floors. Gas sampling was done using static chambers and analysed using a SRI 8610C gas chromatography machine. The highest CH4 fluxes were from the manure of herded goats on earth floors (HE). The highest CO2 fluxes were from manure of herded goats that were housed on slatted floors (HS): The highest N2O fluxes were from the manure of tethered goats that were sheltered on slatted floors (TS). It was concluded from the first experiment that goat manure is an important source of GHG that is influenced by grazing and floor type management systems. The second experiment was done to evaluate greenhouse gas (GHG) emission from disused (DP) vs occupied goat pens (OP) with earth (EF) or slatted (SF) floors. Eight previously occupied pens were used in this study. Sixteen goats were randomly put in the pens labelled occupied pens with earth floors (OPEF) and occupied pens with slatted floors (OPSF) resulting in four goats per OP. There were month by pen use; month by floor type; and use by floor type interaction effects (P < 0.05) on CH4 production. Floor type, use (DP vs OP) and month affected (P < 0.05) CH4 production. Manure on earth floors of occupied pens (OPEF) emitted the highest (P < 0.05) methane followed by earth floors of disused pens (DPEF). The least CH4 was emitted from DPSF. There was pen use (DP vs OP) by floor type interaction effect (P < 0.05) on CO2 emission. Floor type and pen use affected (P < 0.05) CO2 production. Manure in occupied pens with slatted floors (OPSF) produced the highest CO2 and the least CO2 was from disused pens with slatted floors (DPSF). Floor type and month influenced (P < 0.05) N2O production. It was concluded that continued use of goat pens increased GHG emissions. However, disused goat pens with manure remain an important source of GHG as they continued to emit quantifiable levels of CH4, CO2 and N2O. The third experiment was done to determine the effects of grazing and housing management systems on the concentrations of blood metabolites, activity of creatine kinase, body weight (BW), body condition score (BCS) and carcass characteristics using 48 castrated Nguni goats. Tethered goats had higher (P<0.05): urea, creatinine, total protein, globulin concentrations and more activity of CK than herded goats. On the other hand, herded goats had higher (P<0.05) plasma glucose (H: 3.39±0.020 vs T: 3.23±0.020 mmol/L), serum albumin (H: 15.30±0.147 vs T: 14.82±0.144 g/L) concentration. It was concluded that serum concentrations of all the metabolites and the activity of CK were sensitive to grazing system. Herding of goats promoted more glucose concentration and preservation of muscle protein and hence superior body weights and BCS than tethered goats. The fourth experiment was done to determine the effects of grazing and housing management systems on the activity of creatine kinase (CK) and cortisol concentration, and chevon quality. Further, the effect of muscle type [Muscularis longissimus thoracis et. Lumborum (LTL) and triceps brachii (TB)] on chevon quality was also investigated. Forty eight castrated Nguni goats aged 6- 7 months were randomly allocated to tethering (n=24) and herding (n=24) treatments. Floor type affected meat (P<0.05) pH24. Tethered goats had higher (P < 0.05) L* and CL percent than herded goats. Muscle type had a significant influence (P < 0.05) on a*, WBSF and pH. It was observed and concluded that T resulted in higher L* and more CL percent whilst SF lowered the pH24 of chevon; at the same time meat from the TB muscle was more tender, despite having a higher pH24 and more CL percent relative to LTH cuts. The fifth experiment was done to determine the effect of grazing and floor type on fatty acid composition of chevon from tethered and herded Nguni goats. Floor type had no effect on fatty acid composition. Oleic, stearic, palmitic and linoleic were the major fatty acids. Chevon from tethered goats had higher (P < 0.05) proportions of long chain polyunsaturated fatty acids (PUFAs): eicosapentaenoic, docosapentaenoic, docosahexaenoic, conjugated linoleic acid (CLA) and more total SFA, total PUFA, total Omega- 6 and total omega 3 fatty acids than herded goats. On the other hand, chevon from herded goats had more (P < 0.05) C18:1c9, C18:2t9, 12 (n-6), C20:3c11, 14, 17 (n-3) and total MUFA than tethered goats. However, there was no difference (P > 0.05) in the PUFA: SFA and n-6/n-3 fatty acid ratios between the herded and tethered goats. It was concluded that tethering promoted higher proportions of beneficial PUFAs which were more in TB than LTH muscle. The overall conclusions from the whole study were that goat manure is an important source of GHG with high CH4 being emitted from earth floors of herded goats. High CO2 and N2O are emitted from SF of herded and tethered goats, respectively. There were no major differences in meat quality between herded and tethered goats. However, herding promoted more live weight gain than tethering. On the other hand, tethering promoted more beneficial fatty acids. Therefore depending on the objective of the enterprise, smallholder farmers can safely tether or herd their goats. Further, there is need to develop technologies to harness CH4 from goat manure.
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Effects of Pteronia incana (Blue bush) invasion on grass biomass production, soil chemical characteristics and peoples' livelihoods in Ngqushwa communal rangelands, Eastern Cape
- Authors: Ntutha, T
- Date: 2016
- Subjects: Invasive plants -- South Africa -- Eastern Cape Shrublands -- South Africa -- Eastern Cape Rangelands -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/4682 , vital:28493
- Description: The objective of the study was to assess the farmers’ knowledge and perceptions of P. incana invasion and impacts on rangeland, livestock production and livelihood in four communal grazing areas. Twenty households per village that own livestock were randomly selected to conduct an interview using an open and closed ended questionnaire. In each household, an old and knowledgeable respondent about P. incana invasion was used as a selection criterion. For scientific assessment of range condition, the experiment was conducted in a 270 m x 100 m trial plots that were established in 2014. The area was divided into 3 open and 3 fenced subplots each having an area of 45 m x 100 m established laid down a slope gradient. Each plot was divided into four equal subplots to apply the control treatments. Therefore, the trial layout was a stratified block experiment of four treatments replicated three times. The four control treatments applied to P. incana invasion were chopping and resting (CR), resting without chopping (RWOC), grazing and chopping (GC), grazing without chopping (GWOC). A step point method was applied in each transect to monitor species composition and P. incana cover. On the fenced plots two exclosures of 1mx1m size were randomly distributed to make sure that resting is maintained. Within exclosures 0.25 m2quadrates in each were put randomly to measure biomass. Density of P. incana was determined from 5 m x 5 m quadrates that were placed randomly within each sub-plot. The density and height of individual shrub plant was measured. All rooted live woody plants were recorded and counted in each quadrate. The results revealed that species composition was not affected (P>0.05) by the treatments applied. The herbage height and basal cover had greater values (P<0.05) under rested plus chopping (CR) treatment followed by grazed plus chopping (GC). Grass biomass production and essential nutrients showed a greater concentration (P<0.05) under CR treatment than any treatments applied. The results on perceptions revealed that goats had the highest mean household holding number than any other livestock species across the selected villages in all the villages. Respondents indicated that both goats and cattle had equal or more importance than sheep. Three out of four villages raised cattle primarily for income generation and secondarily for cultural purposes like slaughtering during weddings ceremonies, amazila and also during woman circumcisions (Ntonjane). Similarly, goats and sheep were primarily raised for cash income generation. The results revealed that livestock population decreased over ten to twenty years (one-two decades) and poor rangeland condition is assumed to be the cause of the situation as feed is the key factor for production. The area was dominated by the grasses rather than by browse woody species so the above results are possible as the P. incana is more competitive than grasses. It was concluded that P. incana is detrimental to both rangeland and animal production. Forage and animal performance as well as farmers’ economic status (livelihoods) were negatively affected by P. incana invasion and the scientific assessment reported it to be detrimental to agriculture. Thus chopping and resting (CR) treatment resulted in a greater improvement of the basal cover, biomass production, soil nutrients as well as grass height than the other treatments applied. Therefore it can be concluded that chopping and resting the veld invaded with P. incana can improve the range and consequently animal production.
- Full Text:
- Authors: Ntutha, T
- Date: 2016
- Subjects: Invasive plants -- South Africa -- Eastern Cape Shrublands -- South Africa -- Eastern Cape Rangelands -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/4682 , vital:28493
- Description: The objective of the study was to assess the farmers’ knowledge and perceptions of P. incana invasion and impacts on rangeland, livestock production and livelihood in four communal grazing areas. Twenty households per village that own livestock were randomly selected to conduct an interview using an open and closed ended questionnaire. In each household, an old and knowledgeable respondent about P. incana invasion was used as a selection criterion. For scientific assessment of range condition, the experiment was conducted in a 270 m x 100 m trial plots that were established in 2014. The area was divided into 3 open and 3 fenced subplots each having an area of 45 m x 100 m established laid down a slope gradient. Each plot was divided into four equal subplots to apply the control treatments. Therefore, the trial layout was a stratified block experiment of four treatments replicated three times. The four control treatments applied to P. incana invasion were chopping and resting (CR), resting without chopping (RWOC), grazing and chopping (GC), grazing without chopping (GWOC). A step point method was applied in each transect to monitor species composition and P. incana cover. On the fenced plots two exclosures of 1mx1m size were randomly distributed to make sure that resting is maintained. Within exclosures 0.25 m2quadrates in each were put randomly to measure biomass. Density of P. incana was determined from 5 m x 5 m quadrates that were placed randomly within each sub-plot. The density and height of individual shrub plant was measured. All rooted live woody plants were recorded and counted in each quadrate. The results revealed that species composition was not affected (P>0.05) by the treatments applied. The herbage height and basal cover had greater values (P<0.05) under rested plus chopping (CR) treatment followed by grazed plus chopping (GC). Grass biomass production and essential nutrients showed a greater concentration (P<0.05) under CR treatment than any treatments applied. The results on perceptions revealed that goats had the highest mean household holding number than any other livestock species across the selected villages in all the villages. Respondents indicated that both goats and cattle had equal or more importance than sheep. Three out of four villages raised cattle primarily for income generation and secondarily for cultural purposes like slaughtering during weddings ceremonies, amazila and also during woman circumcisions (Ntonjane). Similarly, goats and sheep were primarily raised for cash income generation. The results revealed that livestock population decreased over ten to twenty years (one-two decades) and poor rangeland condition is assumed to be the cause of the situation as feed is the key factor for production. The area was dominated by the grasses rather than by browse woody species so the above results are possible as the P. incana is more competitive than grasses. It was concluded that P. incana is detrimental to both rangeland and animal production. Forage and animal performance as well as farmers’ economic status (livelihoods) were negatively affected by P. incana invasion and the scientific assessment reported it to be detrimental to agriculture. Thus chopping and resting (CR) treatment resulted in a greater improvement of the basal cover, biomass production, soil nutrients as well as grass height than the other treatments applied. Therefore it can be concluded that chopping and resting the veld invaded with P. incana can improve the range and consequently animal production.
- Full Text:
Electrical power output estimation model for a conical diffuser augmented wind turbine
- Authors: Masukume, Peace-Maker
- Date: 2016
- Subjects: Wind power , Wind turbines , Renewable energy sources
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10353/1517 , vital:27404
- Description: Energy is integral to the quality of life of any society. However, meeting the demand for energy sustainably is the main challenge facing humanity. In general, non-renewable energy resources are used to supply the ever increasing energy demand. However, the extraction and processing of these resources is accompanied by the production of wastes which are a health hazard and impact negatively on climate change. Considering the finite nature of non-renewable sources, the environmental concerns which are associated with their usage and ensuring energy security, renewable energy sources have been brought in the energy supply chain. Wind energy is one of the renewable energy sources which has been supplying electrical energy to the ever increasing energy demand of humanity. Wind energy technology is a mature technology which over and above the bare (conventional) wind turbine technology has seen the development of duct augmented wind turbines. Ducts are used to encase wind turbine rotors to augment the power output of wind turbines especially in low wind speed areas. Though the technology has been under study for decades now, research indicates that there is no known model to estimate the power output of a diffuser augmented wind turbine. This thesis presents the development of the conical Diffuser Augmented Wind Turbine (DAWT) power output estimation model and its validation.
- Full Text:
- Authors: Masukume, Peace-Maker
- Date: 2016
- Subjects: Wind power , Wind turbines , Renewable energy sources
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10353/1517 , vital:27404
- Description: Energy is integral to the quality of life of any society. However, meeting the demand for energy sustainably is the main challenge facing humanity. In general, non-renewable energy resources are used to supply the ever increasing energy demand. However, the extraction and processing of these resources is accompanied by the production of wastes which are a health hazard and impact negatively on climate change. Considering the finite nature of non-renewable sources, the environmental concerns which are associated with their usage and ensuring energy security, renewable energy sources have been brought in the energy supply chain. Wind energy is one of the renewable energy sources which has been supplying electrical energy to the ever increasing energy demand of humanity. Wind energy technology is a mature technology which over and above the bare (conventional) wind turbine technology has seen the development of duct augmented wind turbines. Ducts are used to encase wind turbine rotors to augment the power output of wind turbines especially in low wind speed areas. Though the technology has been under study for decades now, research indicates that there is no known model to estimate the power output of a diffuser augmented wind turbine. This thesis presents the development of the conical Diffuser Augmented Wind Turbine (DAWT) power output estimation model and its validation.
- Full Text:
Environmental factors influencing ecotonal changes in an indigenous forest in the Keiskammahoek Forest Estate, Eastern Cape, South Africa
- Authors: Kiva, Luthando
- Date: 2016
- Subjects: Forest ecology -- South Africa -- Eastern Cape Forests and forestry -- South Africa -- Eastern Cape Forest conservation -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/2743 , vital:28071
- Description: This study investigates environmental factors influencing ecotonal changes in the Dontsa Forest Management Unit (FMU) of the Keiskammahoek Estate Forest which is located in the Amatole Mountains of the Eastern Cape in the Republic of South Africa. The patterns of forest edge movement were analysed to show trends of forest edge movement from 1975 to 1985, 1975 to 1992, 1975 to 2002, 1975 to 2014, 1985 to 1992, 1985 to 2002, 1985 to 2014, 1992 to 2002, 1992 to 2014 and 2002 to 2014 by digitizing and assessing the scale of forest edge movement using georeferenced aerial photographs. Belt transects were established in six sites that were selected on the basis of physiographic elements for determination of the driving forces of forest ecotonal changes. The results of the study show that the forest edge moved positive towards the grassland biome while in other sites there was contraction of the forest edge from 1975 to 2014. The findings of the study show that some forest patches moved with few individual pioneer species towards the grassland while indigenous species dominated in the ecotone area of the forest in other research sites. D whyteana, A latifolius, R melanophloes, A facultus, R prenoides, C aurea, C bispinosa, C inerme, and S martina are the plant species with high density in the forest ecotone while A latifolius, R prenoides, R melanophloes were highly distributed along the grassland area. The results also show that harvesting of Pinus patula and illegal harvesting of understory species are major factors that result in ecotonal changes of Dontsa FMU. The research sites adjacent to residential areas have experienced reduction of ecotone area as compared to the research sites in high altitude areas of the Amathole Mountains where there is less disturbance. The eastern facing aspect of the forest exhibited a high density of plants in the forest ecotone as compared to the west facing slope.
- Full Text:
- Authors: Kiva, Luthando
- Date: 2016
- Subjects: Forest ecology -- South Africa -- Eastern Cape Forests and forestry -- South Africa -- Eastern Cape Forest conservation -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/2743 , vital:28071
- Description: This study investigates environmental factors influencing ecotonal changes in the Dontsa Forest Management Unit (FMU) of the Keiskammahoek Estate Forest which is located in the Amatole Mountains of the Eastern Cape in the Republic of South Africa. The patterns of forest edge movement were analysed to show trends of forest edge movement from 1975 to 1985, 1975 to 1992, 1975 to 2002, 1975 to 2014, 1985 to 1992, 1985 to 2002, 1985 to 2014, 1992 to 2002, 1992 to 2014 and 2002 to 2014 by digitizing and assessing the scale of forest edge movement using georeferenced aerial photographs. Belt transects were established in six sites that were selected on the basis of physiographic elements for determination of the driving forces of forest ecotonal changes. The results of the study show that the forest edge moved positive towards the grassland biome while in other sites there was contraction of the forest edge from 1975 to 2014. The findings of the study show that some forest patches moved with few individual pioneer species towards the grassland while indigenous species dominated in the ecotone area of the forest in other research sites. D whyteana, A latifolius, R melanophloes, A facultus, R prenoides, C aurea, C bispinosa, C inerme, and S martina are the plant species with high density in the forest ecotone while A latifolius, R prenoides, R melanophloes were highly distributed along the grassland area. The results also show that harvesting of Pinus patula and illegal harvesting of understory species are major factors that result in ecotonal changes of Dontsa FMU. The research sites adjacent to residential areas have experienced reduction of ecotone area as compared to the research sites in high altitude areas of the Amathole Mountains where there is less disturbance. The eastern facing aspect of the forest exhibited a high density of plants in the forest ecotone as compared to the west facing slope.
- Full Text:
Environmental management in military activities of the South African National Defence Force
- Authors: Magagula, Hezekiel Bheki
- Date: 2016
- Subjects: South Africa -- South African Defence Force Military bases -- Environmental aspects Hazardous waste site remediation
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10353/5927 , vital:29430
- Description: This thesis presents research findings on current environmental management practices used by the South African National Defence Force (SANDF). Two case studies are presented; the first is the Grahamstown Military Installation (6 South African Infantry Battalion) and the second the South African Army Combat Training Centre (42 Brigade). A combination of three sampling techniques, namely, the stratified, purposive and link-tracing (snowball) samplings were employed and structured questionnaires and semi-structured interviews with key SANDF officials were used as primary data. In addition, secondary data sources in the form of inter alia the first and second editions of the Environmental Management Plan for Defence (2001 & 2008 respectively); the development and implementation of environmental education and training in the military: a joint United States-Republic of South Africa Environmental Working Group Project (2003); Overarching Strategic Statement for 2011; Integrated Environmental Management Information Series: Linking Environmental Impact Assessment and Environmental Management Systems (2004); The Department of Defence Strategic Plan (2010): Republic of South Africa and the South African Defence Review (2012) were studied. In addition, field observations were also carried out within the two military installations training areas between July, 2011 and November, 2013. Primary data on the conditions of training areas, waste removal after training exercises, pollution prevention measures, and rehabilitation efforts to restore degraded training ranges were collected. Furthermore, composite surface sediment samples and water samples were collected for analysis. A total of fifty-six surface sediments and thirty water samples were analysed for the concentrations of heavy metals using the ICP-MS. These attitudes and perceptions are predominantly positive. Statistical analysis (ANOVA) did not reveal any significant differences in responses, especially, between military ranks. However, sporadic significant differences were found in five of the statements in the questionnaire from respondents with different service periods. Furthermore, the emphasis placed on environmental protection within defence force activities worldwide has compelled the South African Department of Defence and Military Veterans (DoDMV) to regulate the management of the environment within its properties. Yet, these efforts have faced numerous challenges ranging from financial to human resource deficiencies. It was found that environmental management practices and programmes at different military installations vary significantly. Consequently, six environmental management programmes were identified at South African Army Combat Training Centre, while only one environmental management programme was identified at Grahamstown Military Installation. This programme is a collaborative effort between the SANDF/DoDMV and the Department of Water Affairs and Sanitation to eradicate invasive alien plant species. In the light of the analysis of official documents and interviews with respondents, it was established that the DoDMV does not have a budget for environmental management services and environmental management is yet to be incorporated into formal military training programmes. There is a severe shortage of environmentally qualified and knowledgeable personnel within the SANDF. Subsequently, all these drawbacks lead to the failure of the implementation of the Defence Force’s Environmental Management System (EMS) and inadequate management of the environment at military installations of the SANDF. Consequently, these challenges have severely compromised the commitment of the SANDF to honour its environmental management obligations. Such deficiencies tend to undermine the sustainable utilisation of the national assets entrusted to the Defence Force. Therefore, this thesis argues that environmental management programmes for the SANDF are based on a very weak foundation. This thesis proposes an ideal model for the successful implementation of the EMS and management of the environment at SANDF military installations. The analysis of water and soil samples led to the identification and quantification of heavy mental pollutants. The concentrations of heavy metals in water samples were varying between <0.01 to <0.05mg/l, but generally constant. Thus, no significant or meaningful statistical results were obtained. Pearson’s correlation coefficient was used to analyse the concentrations of heavy metals obtained in the sediment samples.
- Full Text:
- Authors: Magagula, Hezekiel Bheki
- Date: 2016
- Subjects: South Africa -- South African Defence Force Military bases -- Environmental aspects Hazardous waste site remediation
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10353/5927 , vital:29430
- Description: This thesis presents research findings on current environmental management practices used by the South African National Defence Force (SANDF). Two case studies are presented; the first is the Grahamstown Military Installation (6 South African Infantry Battalion) and the second the South African Army Combat Training Centre (42 Brigade). A combination of three sampling techniques, namely, the stratified, purposive and link-tracing (snowball) samplings were employed and structured questionnaires and semi-structured interviews with key SANDF officials were used as primary data. In addition, secondary data sources in the form of inter alia the first and second editions of the Environmental Management Plan for Defence (2001 & 2008 respectively); the development and implementation of environmental education and training in the military: a joint United States-Republic of South Africa Environmental Working Group Project (2003); Overarching Strategic Statement for 2011; Integrated Environmental Management Information Series: Linking Environmental Impact Assessment and Environmental Management Systems (2004); The Department of Defence Strategic Plan (2010): Republic of South Africa and the South African Defence Review (2012) were studied. In addition, field observations were also carried out within the two military installations training areas between July, 2011 and November, 2013. Primary data on the conditions of training areas, waste removal after training exercises, pollution prevention measures, and rehabilitation efforts to restore degraded training ranges were collected. Furthermore, composite surface sediment samples and water samples were collected for analysis. A total of fifty-six surface sediments and thirty water samples were analysed for the concentrations of heavy metals using the ICP-MS. These attitudes and perceptions are predominantly positive. Statistical analysis (ANOVA) did not reveal any significant differences in responses, especially, between military ranks. However, sporadic significant differences were found in five of the statements in the questionnaire from respondents with different service periods. Furthermore, the emphasis placed on environmental protection within defence force activities worldwide has compelled the South African Department of Defence and Military Veterans (DoDMV) to regulate the management of the environment within its properties. Yet, these efforts have faced numerous challenges ranging from financial to human resource deficiencies. It was found that environmental management practices and programmes at different military installations vary significantly. Consequently, six environmental management programmes were identified at South African Army Combat Training Centre, while only one environmental management programme was identified at Grahamstown Military Installation. This programme is a collaborative effort between the SANDF/DoDMV and the Department of Water Affairs and Sanitation to eradicate invasive alien plant species. In the light of the analysis of official documents and interviews with respondents, it was established that the DoDMV does not have a budget for environmental management services and environmental management is yet to be incorporated into formal military training programmes. There is a severe shortage of environmentally qualified and knowledgeable personnel within the SANDF. Subsequently, all these drawbacks lead to the failure of the implementation of the Defence Force’s Environmental Management System (EMS) and inadequate management of the environment at military installations of the SANDF. Consequently, these challenges have severely compromised the commitment of the SANDF to honour its environmental management obligations. Such deficiencies tend to undermine the sustainable utilisation of the national assets entrusted to the Defence Force. Therefore, this thesis argues that environmental management programmes for the SANDF are based on a very weak foundation. This thesis proposes an ideal model for the successful implementation of the EMS and management of the environment at SANDF military installations. The analysis of water and soil samples led to the identification and quantification of heavy mental pollutants. The concentrations of heavy metals in water samples were varying between <0.01 to <0.05mg/l, but generally constant. Thus, no significant or meaningful statistical results were obtained. Pearson’s correlation coefficient was used to analyse the concentrations of heavy metals obtained in the sediment samples.
- Full Text:
Evaluating farmers' perceptions and the impact of bush encroachment on herbaceous vegetation and soil nutrients in Sheshegu communal rangelands of the Eastern Cape, South Africa
- Authors: Tokozwayo, Sive
- Date: 2016
- Subjects: Range management -- South Africa -- Eastern Cape Brush -- Control -- South Africa -- Eastern Cape Communal rangelands -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/2733 , vital:28066
- Description: Communal rangelands occupy 13 percent of the agricultural land in South Africa, and these rangelands serve as a source of feed to livestock. These areas are threatened by bush encroachment due to poor rangeland management. This study was conducted in Alice (Sheshegu communal area) and the objective was to assess farmers' perceptions and the impact of bush encroachment on herbaceous vegetation and soil nutrients. Structured questionnaires were used to assess indigenous knowledge of communal farmers on the impact of bush encroachment on rangelands. Fourty (40) respondents from households who owned livestock were randomly selected and interviewed at Sheshegu village. About 89 percent of communal farmers perceived that change of their grasslands to encroached savannas was caused by unreliable rainfall, prolonged drought, and poor rangeland management. Rangeland assessment was performed at four sites (Scattered, Moderate, Mixed and Dense bushland. 100mx50m were demarcated per site, and four 100m transects were laid parallel to each other, 30m apart. The step point method was used to determine both species composition and basal cover. Biomass production was determined by harvesting forage within randomly-paced a 0.25m2 quadrats. Aristada congesta and Eragrostis obtuse were the most dominating grass species in dense, mixed and moderate bushland. Scattered bushland was dominated by Themeda triandra. Biomass production increases with increase in bush density and basal cover improved from winter to summer season. Increaser grass species increase with the increase in bush density, this indicated that the rangeland was poorly managed and palatable species were being replaced by less palatable ones. Species composition of woody plants was determined within a 200m2 belt transect in all sites. Maytenus polycantha, Aloe ferox, Erehia rigida and lucas capensis were the most dominant woody species in Mixed bushland while Acacia karroo was most dominated in Scattered, Moderate and Dense bushland. Woody density showed no significant differences (P> 0.05) between Dense, Mixed and Moderate bushland. Woody plant density in all these sites exceed 2500 plants/ha, which showed that the rangeland was encroached with woody plants. Soil nutrient content was determined for each site. Five samples of soil were collected per site to test the effect of bush encroachment on soil nutrient composition. The concentration of nitrogen and soil pH increased with increase in bush density, while that of potassium, phosphorous, magnesium, sodium and sulphur showed no significant differences (P>0.05) across all homogenous vegetation units. The study revealed that Sheshegu communal farmers are aware of both negative and positive effects of encroachment on their community, and also argue that the government is not willing to support communal farmers in the process of reducing the spread of bush encroachment. It was concluded that the concentration of nitrogen and soil pH increased with increasing bush density and that bush encroachment had negative effects on herbaceous species. It was recommended that communal farmers should be formulating rangeland rules and regulations to manage rangeland resources. Rangeland rules and regulations should be based on the management of livestock management during grazing and harvesting of rangeland resources. The harvesting of woody and good timing of application of fire can also reduce the spread of bush encroachment. Veld resting and rotational grazing can also improve fuel load which is required for burning of woody plants. Furthermore, Department of agriculture should also provide workshops or training to communal farmers use different approaches or cheap ways of reducing bush encroachment and conserving rangeland resources.
- Full Text:
- Authors: Tokozwayo, Sive
- Date: 2016
- Subjects: Range management -- South Africa -- Eastern Cape Brush -- Control -- South Africa -- Eastern Cape Communal rangelands -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/2733 , vital:28066
- Description: Communal rangelands occupy 13 percent of the agricultural land in South Africa, and these rangelands serve as a source of feed to livestock. These areas are threatened by bush encroachment due to poor rangeland management. This study was conducted in Alice (Sheshegu communal area) and the objective was to assess farmers' perceptions and the impact of bush encroachment on herbaceous vegetation and soil nutrients. Structured questionnaires were used to assess indigenous knowledge of communal farmers on the impact of bush encroachment on rangelands. Fourty (40) respondents from households who owned livestock were randomly selected and interviewed at Sheshegu village. About 89 percent of communal farmers perceived that change of their grasslands to encroached savannas was caused by unreliable rainfall, prolonged drought, and poor rangeland management. Rangeland assessment was performed at four sites (Scattered, Moderate, Mixed and Dense bushland. 100mx50m were demarcated per site, and four 100m transects were laid parallel to each other, 30m apart. The step point method was used to determine both species composition and basal cover. Biomass production was determined by harvesting forage within randomly-paced a 0.25m2 quadrats. Aristada congesta and Eragrostis obtuse were the most dominating grass species in dense, mixed and moderate bushland. Scattered bushland was dominated by Themeda triandra. Biomass production increases with increase in bush density and basal cover improved from winter to summer season. Increaser grass species increase with the increase in bush density, this indicated that the rangeland was poorly managed and palatable species were being replaced by less palatable ones. Species composition of woody plants was determined within a 200m2 belt transect in all sites. Maytenus polycantha, Aloe ferox, Erehia rigida and lucas capensis were the most dominant woody species in Mixed bushland while Acacia karroo was most dominated in Scattered, Moderate and Dense bushland. Woody density showed no significant differences (P> 0.05) between Dense, Mixed and Moderate bushland. Woody plant density in all these sites exceed 2500 plants/ha, which showed that the rangeland was encroached with woody plants. Soil nutrient content was determined for each site. Five samples of soil were collected per site to test the effect of bush encroachment on soil nutrient composition. The concentration of nitrogen and soil pH increased with increase in bush density, while that of potassium, phosphorous, magnesium, sodium and sulphur showed no significant differences (P>0.05) across all homogenous vegetation units. The study revealed that Sheshegu communal farmers are aware of both negative and positive effects of encroachment on their community, and also argue that the government is not willing to support communal farmers in the process of reducing the spread of bush encroachment. It was concluded that the concentration of nitrogen and soil pH increased with increasing bush density and that bush encroachment had negative effects on herbaceous species. It was recommended that communal farmers should be formulating rangeland rules and regulations to manage rangeland resources. Rangeland rules and regulations should be based on the management of livestock management during grazing and harvesting of rangeland resources. The harvesting of woody and good timing of application of fire can also reduce the spread of bush encroachment. Veld resting and rotational grazing can also improve fuel load which is required for burning of woody plants. Furthermore, Department of agriculture should also provide workshops or training to communal farmers use different approaches or cheap ways of reducing bush encroachment and conserving rangeland resources.
- Full Text:
Evaluating revenue collection and allocation challenges faced by Ndlambe Local Municipality (Eastern Cape Province)
- Pieters, Nonkqubela Ntomboxolo
- Authors: Pieters, Nonkqubela Ntomboxolo
- Date: 2016
- Subjects: Municipal corporations -- Taxation -- South Africa -- Eastern Cape Municipal finance -- South Africa -- Eastern Cape Local government -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MPA
- Identifier: http://hdl.handle.net/10353/4442 , vital:28333
- Description: In this project, the researcher evaluates revenue collection and allocation challenges faced by South African Municipalities in the case of NDLAMBE Local Municipality and this research project consist of five Chapters. Chapter one introduces and outlines the setting of the study and also provides the problem statement, research questions, research objectives and significance of the study. The chapter further clarifies the key concepts and terms that were used in the study, delimitation and limitations of the study. Chapter two provides a critical discussion of the concept of revenue collection and allocation, focusing on the various perspective of revenue. A source of municipal revenue and revenue collection and allocation model is explained as well as theoretical framework that guides it. Chapter three gives an account of the research design and methodology used in the study and in this study the researcher used the qualitative research approach as a data collection technique and document surveys were used because it is cheap and it saves time. The chapter also highlights the revenue collection and allocation applicable to the study and conclude by discussing the data analysis techniques that were used in the empirical study. Chapter four dealt with the data analysis of data collected presentation and discussion of the results and the researcher analyzed data using the filtering technique and content analysis. Chapter five drew conclusions and made scientific recommendations based on the research findings.The municipality is largely dependent on grants from upper spheres of government and generate less than 20 percent of current expenditures from own resources. As a whole, the revenue collection and allocation challenge of the NDLAMBE Local Municipality, given the current scenario of flows, is a reason for concern. Due to historical and political factors, local governments in South Africa differ substantially in terms of potential revenue base, but it may be that in many cases potential revenue is not exploited and that the high level of dependency on grants, high level of bad debts and lack of political will to be more self-reliant is the result of poor revenue in NDLAMBE Local Municipality.
- Full Text:
- Authors: Pieters, Nonkqubela Ntomboxolo
- Date: 2016
- Subjects: Municipal corporations -- Taxation -- South Africa -- Eastern Cape Municipal finance -- South Africa -- Eastern Cape Local government -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MPA
- Identifier: http://hdl.handle.net/10353/4442 , vital:28333
- Description: In this project, the researcher evaluates revenue collection and allocation challenges faced by South African Municipalities in the case of NDLAMBE Local Municipality and this research project consist of five Chapters. Chapter one introduces and outlines the setting of the study and also provides the problem statement, research questions, research objectives and significance of the study. The chapter further clarifies the key concepts and terms that were used in the study, delimitation and limitations of the study. Chapter two provides a critical discussion of the concept of revenue collection and allocation, focusing on the various perspective of revenue. A source of municipal revenue and revenue collection and allocation model is explained as well as theoretical framework that guides it. Chapter three gives an account of the research design and methodology used in the study and in this study the researcher used the qualitative research approach as a data collection technique and document surveys were used because it is cheap and it saves time. The chapter also highlights the revenue collection and allocation applicable to the study and conclude by discussing the data analysis techniques that were used in the empirical study. Chapter four dealt with the data analysis of data collected presentation and discussion of the results and the researcher analyzed data using the filtering technique and content analysis. Chapter five drew conclusions and made scientific recommendations based on the research findings.The municipality is largely dependent on grants from upper spheres of government and generate less than 20 percent of current expenditures from own resources. As a whole, the revenue collection and allocation challenge of the NDLAMBE Local Municipality, given the current scenario of flows, is a reason for concern. Due to historical and political factors, local governments in South Africa differ substantially in terms of potential revenue base, but it may be that in many cases potential revenue is not exploited and that the high level of dependency on grants, high level of bad debts and lack of political will to be more self-reliant is the result of poor revenue in NDLAMBE Local Municipality.
- Full Text:
Evaluation of flocculating potentials and charecterization of bioflocculants produced by three bacterial isolates from Algoa Bay, South Africa
- Authors: Okaiyeto, Kunle
- Date: 2016
- Subjects: Flocculation Water -- Purification -- Flocculation Water quality management
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10353/2633 , vital:27947
- Description: Flocculation has been widely adopted as one of the most effective methods to remove colloidal particles in water or wastewater treatment. Synthetic flocculants are conventionally used because of their high flocculating efficiency and cost-effectiveness. However, they have been reported to have hazardous properties and implicated in some serious health problems including senile dementia and neuro-toxicity, as well as being recalcitrant in the environment. Consequently, efforts are being geared away from the use of synthetic flocculants in water and wastewater treatment. Hence, the need for safe and eco-friendly flocculants has become imperative. Compared with synthetic flocculants, bioflocculants have special advantages such as safety, biodegradability and harmlessness to the environment and humans; attributes which make them potential alternatives in water treatment, downstream as well as fermentation processes. In the current study, the potentials of bacterial isolates recovered from Algoa Bay in the Eastern Cape Province of South Africa for bioflocculant production were investigated. The bacterial isolates were identified by polymerase chain reaction (PCR) as belonging to the Bacillus genus. The analysis of 16S ribosomal deoxyribonucleic acid (rDNA) nucleotide sequence of isolate M72 showed 99 percent similarity to Bacillus toyonensis strain BCT-7112 and was deposited in the GenBank as Bacillus toyonensis strain AEMREG6 with accession number KP406731. Likewise, the 16S rDNA nucleotide sequences of isolates M69 and M67 showed 98 percent sequence similarity to Bacillus licheniformis strain W7 and Bacillus algicola strain QD43 respectively; and M67 isolate was subsequently deposited in the GenBank as Bacillus sp. AEMREG7 with accession number KF933697.1. The results of the nutritional requirements and fermentation conditions revealed that optimum inoculum size for REG-6 production was 4 percent (v/v), while 5 percent (v/v) and 3 percent (v/v) were most favourable for MBF-W7 and MBF-UFH production respectively. Glucose was the best carbon source for the production of bioflocculants (REG-6 and MBF-UFH) by Bacillus toyonensis AEMREG6 and Bacillus sp. AEMREG7 respectively, while maltose supported optimum bioflocculant (MBF-W7) production by Bacillus specie. Inorganic nitrogen (NH4NO3) was the favoured nitrogen source for both REG-6 and MBF-W7 production, while mixed nitrogen sources [yeast extract + urea + (NH4)2SO4] supported the maximum production of MBF-UFH. The initial medium pH for REG-6 was 5, while MBF-W7 and MBF-UFH were both maximally produced at the initial pH of 6. After a 96 h cultivation period under optimal culture conditions, 3.2 g of purified REG-6 with a maximum flocculating activity of 77 percent was recovered from 1 L fermented broth of Bacillus toyonensis AEMREG6. Yields of 3.8 g and 1.6 g pure bioflocculants with the respective highest flocculating activities of 94.9 percent and 83.2 percent were also obtained from 1 L, 72 h-fermented broths of Bacillus licheniformis and Bacillus sp. AEMREG7 respectively. Furthermore, all the three bioflocculants (REG-6, MBF-W7 and MBF-UFH), displayed thermal stability within the temperature range of 50 to 100 oC, with strong flocculating activities of over 80 percent against kaolin suspension over a wide range of pH range (3–11) and relatively low dosage requirements of 0.1-03 mg/ml in the presence of divalent cations in the treatment of kaolin clay suspension and Thyme River waters. Chemical composition analyses of the bioflocculants showed them to be glycoproteins with a predominantly polysaccharide backbones as shown by the following carbohydrate/protein (w/w) ratios: 77.8 percent:11.5 percent (REG-6); 73.7 percent:6.2 percent (MBF-W7) and 76 percent:14 percent (MBF-UFH).
- Full Text:
- Authors: Okaiyeto, Kunle
- Date: 2016
- Subjects: Flocculation Water -- Purification -- Flocculation Water quality management
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10353/2633 , vital:27947
- Description: Flocculation has been widely adopted as one of the most effective methods to remove colloidal particles in water or wastewater treatment. Synthetic flocculants are conventionally used because of their high flocculating efficiency and cost-effectiveness. However, they have been reported to have hazardous properties and implicated in some serious health problems including senile dementia and neuro-toxicity, as well as being recalcitrant in the environment. Consequently, efforts are being geared away from the use of synthetic flocculants in water and wastewater treatment. Hence, the need for safe and eco-friendly flocculants has become imperative. Compared with synthetic flocculants, bioflocculants have special advantages such as safety, biodegradability and harmlessness to the environment and humans; attributes which make them potential alternatives in water treatment, downstream as well as fermentation processes. In the current study, the potentials of bacterial isolates recovered from Algoa Bay in the Eastern Cape Province of South Africa for bioflocculant production were investigated. The bacterial isolates were identified by polymerase chain reaction (PCR) as belonging to the Bacillus genus. The analysis of 16S ribosomal deoxyribonucleic acid (rDNA) nucleotide sequence of isolate M72 showed 99 percent similarity to Bacillus toyonensis strain BCT-7112 and was deposited in the GenBank as Bacillus toyonensis strain AEMREG6 with accession number KP406731. Likewise, the 16S rDNA nucleotide sequences of isolates M69 and M67 showed 98 percent sequence similarity to Bacillus licheniformis strain W7 and Bacillus algicola strain QD43 respectively; and M67 isolate was subsequently deposited in the GenBank as Bacillus sp. AEMREG7 with accession number KF933697.1. The results of the nutritional requirements and fermentation conditions revealed that optimum inoculum size for REG-6 production was 4 percent (v/v), while 5 percent (v/v) and 3 percent (v/v) were most favourable for MBF-W7 and MBF-UFH production respectively. Glucose was the best carbon source for the production of bioflocculants (REG-6 and MBF-UFH) by Bacillus toyonensis AEMREG6 and Bacillus sp. AEMREG7 respectively, while maltose supported optimum bioflocculant (MBF-W7) production by Bacillus specie. Inorganic nitrogen (NH4NO3) was the favoured nitrogen source for both REG-6 and MBF-W7 production, while mixed nitrogen sources [yeast extract + urea + (NH4)2SO4] supported the maximum production of MBF-UFH. The initial medium pH for REG-6 was 5, while MBF-W7 and MBF-UFH were both maximally produced at the initial pH of 6. After a 96 h cultivation period under optimal culture conditions, 3.2 g of purified REG-6 with a maximum flocculating activity of 77 percent was recovered from 1 L fermented broth of Bacillus toyonensis AEMREG6. Yields of 3.8 g and 1.6 g pure bioflocculants with the respective highest flocculating activities of 94.9 percent and 83.2 percent were also obtained from 1 L, 72 h-fermented broths of Bacillus licheniformis and Bacillus sp. AEMREG7 respectively. Furthermore, all the three bioflocculants (REG-6, MBF-W7 and MBF-UFH), displayed thermal stability within the temperature range of 50 to 100 oC, with strong flocculating activities of over 80 percent against kaolin suspension over a wide range of pH range (3–11) and relatively low dosage requirements of 0.1-03 mg/ml in the presence of divalent cations in the treatment of kaolin clay suspension and Thyme River waters. Chemical composition analyses of the bioflocculants showed them to be glycoproteins with a predominantly polysaccharide backbones as shown by the following carbohydrate/protein (w/w) ratios: 77.8 percent:11.5 percent (REG-6); 73.7 percent:6.2 percent (MBF-W7) and 76 percent:14 percent (MBF-UFH).
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Evaluation of physiochemical qualities and heavy metal levels of the final effluents of some wastewater treatment facilities in the Eastern Cape Province of South Africa
- Authors: Kulati, Thanduxolo Cullinan
- Date: 2016
- Subjects: Water -- Purification Sewage -- Purification
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/1547 , vital:27417
- Description: Water is the most abundant substance in nature and vital for life activities. The major water sources for use are surface water bodies such as rivers and lakes, and underground aquifers and pore spaces down the water table (Ring, 2003). Water derived from these sources is not necessarily pure since it contains dissolved inorganic and organic substances, living organisms (viruses, bacteria, etc). For these reasons, water intended for domestic uses should be free from toxic substances and microorganisms that are of health significance (WHO, 2005). The availability and quality of water always have played an important role in determining the quality of life. Water quality is closely linked to water use and to the state of economic development (Chennakrishnan et al., 2008). Ground and surface waters can be contaminated by several sources. In urban areas, the careless disposal of industrial effluents and other wastes may contribute greatly to the poor quality of water (Mathuthu et al., 1997). In most developing countries, most areas are located on the watersheds which are the end points of effluents discharged from various industries (Oberholster and Ashton, 2008). South Africa, as a developing country, is experiencing rapid demographic changes due to urbanization, industrialization and population growth. The country has also been identified as being water-scarce, which can lead to a challenge of meeting the increasing water demand due to industrialization and urbanization. Such population growth increase may result in an increase in wastewater output, especially around urban areas.
- Full Text:
- Authors: Kulati, Thanduxolo Cullinan
- Date: 2016
- Subjects: Water -- Purification Sewage -- Purification
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/1547 , vital:27417
- Description: Water is the most abundant substance in nature and vital for life activities. The major water sources for use are surface water bodies such as rivers and lakes, and underground aquifers and pore spaces down the water table (Ring, 2003). Water derived from these sources is not necessarily pure since it contains dissolved inorganic and organic substances, living organisms (viruses, bacteria, etc). For these reasons, water intended for domestic uses should be free from toxic substances and microorganisms that are of health significance (WHO, 2005). The availability and quality of water always have played an important role in determining the quality of life. Water quality is closely linked to water use and to the state of economic development (Chennakrishnan et al., 2008). Ground and surface waters can be contaminated by several sources. In urban areas, the careless disposal of industrial effluents and other wastes may contribute greatly to the poor quality of water (Mathuthu et al., 1997). In most developing countries, most areas are located on the watersheds which are the end points of effluents discharged from various industries (Oberholster and Ashton, 2008). South Africa, as a developing country, is experiencing rapid demographic changes due to urbanization, industrialization and population growth. The country has also been identified as being water-scarce, which can lead to a challenge of meeting the increasing water demand due to industrialization and urbanization. Such population growth increase may result in an increase in wastewater output, especially around urban areas.
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Evaluation of the implementation of local economic development policy for poverty alleviation at selected municipalities in the province of eastern cape (2012-2014)
- Authors: Sidumo, Andile Morrison
- Date: 2016
- Subjects: Economic development -- South Africa -- Eastern Cape Sustainable development -- South Africa -- Eastern Cape Poverty -- Government policy -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10353/7708 , vital:30708
- Description: This thesis evaluated the implementation of local economic development policy for poverty alleviation at selected municipalities in the Province of the Eastern Cape. Poverty is a global challenge of sustainable development that faces communities at international, national, provincial and local sphere of government however, the focus was at selected municipalities in the Province of Eastern Cape. It is the responsibility of municipal authorities to satisfy the basic needs and promote the general welfare of communities at specific geographic areas in the Province which is vastly rural depending on agriculture and partly on the industrial development. Local economic development is a tool or method to alleviate poverty.
- Full Text:
- Authors: Sidumo, Andile Morrison
- Date: 2016
- Subjects: Economic development -- South Africa -- Eastern Cape Sustainable development -- South Africa -- Eastern Cape Poverty -- Government policy -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10353/7708 , vital:30708
- Description: This thesis evaluated the implementation of local economic development policy for poverty alleviation at selected municipalities in the Province of the Eastern Cape. Poverty is a global challenge of sustainable development that faces communities at international, national, provincial and local sphere of government however, the focus was at selected municipalities in the Province of Eastern Cape. It is the responsibility of municipal authorities to satisfy the basic needs and promote the general welfare of communities at specific geographic areas in the Province which is vastly rural depending on agriculture and partly on the industrial development. Local economic development is a tool or method to alleviate poverty.
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Evaluation of the incidence of enteric viruses, Vibrio species and Escherichia coli pathotypes in effluents of two wastewater treatment plants located in Keiskammahoek and Stutterheim in the Eastern Cape Province of South Africa
- Authors: Adefisoye, Martins Ajibade
- Date: 2016
- Subjects: Escherichia coli -- South Africa -- Eastern Cape Water -- Purification -- South Africa -- Eastern Cape Effluent quality -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10353/3165 , vital:28328
- Description: South Africa is currently experiencing water shortage crisis, a challenge that has been attributed not only to the scarcity of freshwater, but also to fast degrading water quality. Factors such as rapid urbanisation, population and economic growth, climate change as well as poor operational and maintenance of many of the exisiting water/wastewater treatment facilities have been acknowledged as important contributors to degrading water quality in the country. Untreated or inadequately treated discharged wastewater effluents constitute point source pollution to many freshwater environments in South Africa. Hence, it becomes imperative to evaluate wastewater discharges in other to protect the scarce freshwater resource, the environment and public health. Over a twelve-month sampling period (September 2012 to August 2013), we assessed the bacteriological, virological and physicochemical qualities of the discharged final effluents of two wastewater treatment facilities in the Eastern Cape Province of South Africa. For the physicochemical assessment, a total of 144 final effluent samples were collected from both the final effluent tanks (FE) and the discharge points (DP) of the treatment facilities. Physicochemical parameters including pH, temperature, turbidity, total dissolved solids (TDS), dissolved oxygen (DO), electrical conductivity (EC) and free chlorine concentration were determined on site while biological oxygen demand (BOD), nitrate (NO3-), nitrite (NO2-), phosphate (PO4-) and chemical oxygen demand (COD) were determined in the laboratory. The bacteriological analysis of the samples was done using standard membrane filtration (MF) technique. Bacterial group assessed included: faecal indicator bacteria (faecal coliforms and E. coli) and Vibrio species, while the antibiotic susceptibility profiles of selected E. coli and Vibrio species isolates against some selected antibiotics commonly used in human therapy and veterinary medicine were determind using the standard agar-disc diffusion method. The occurrence and concentrations of human enteric viruses including: human adenovirus (HAdV), hepatitis A virus (HAV) and rotavirus (RoV) in the samples were determined by TaqMan-based real-time polymerase chain reaction (qPCR) following concentration by adsorption-elution method. The physicochemical characteristics of the samples ranged as follows: pH (6.5 – 7.6), TDS (95 – 171 mg/L), EC (134 – 267 μS/cm), temperature (12 – 27 °C), turbidity (1.5 – 65.7 mg/L), free chlorine (0.08 – 0.72 mg/L), DO (2.06 – 9.81 mg/L), BOD (0.13 – 9.81 mg/L), NO3- (0 – 21.5 mg/L), NO2- (0 – 0.72 mg/L), PO4- (0 – 18.3 mg/L) and COD (27 – 680 mg/L). Some of the characteristic such as pH, TDS, EC, temperature, nitrite and DO (on most instances) complied with recommended guidelines. Other characteristics, however, including turbidity, BOD, nitrate, phosphate and COD fell short of the recommended guidelines. All the 48 samples analysed for bacteriological qualities tested positive for the presence of the bacterial groups with significant (P≤0.05) seasonal variation in their densities. Faecal coliforms were detected in counts ranging from 1 CFU/100ml to 2.7 × 104 CFU/100ml. Presumptive E. coli counts ranged generally between 1 CFU/100ml – 1.4 × 105 CFU/100ml while counts of presumptive Vibrio species ranged between 4 CFU/100ml – 1.4 × 104 CFU/100ml. Molecular identification of the presumptive isolates by polymerase chain reactions PCR gave positive reaction rates of 76.2 percent (381/500) and 69.8 percent (279/400) for E.coli and Vibrio species respectively. The antibiotic susceptibility profiling of 205 PCR-confirmed Vibiro isolates against 18 commomly used antibiotics showed resistance frequencies ranging from 0.5 percent (imipenem) to 96.1 percent (penicillin G) at recommended breakpoint concentrations. Eighty-one percent (166/205) of the Vibrio isolates showed multidrug resistance (resistance to 3 or more antibiotics) with the most common multiple antibiotic resistance phenotype (MARP) being AP-T-TM-SMX-PG-NI-PB, occurring in 8 isolates.
- Full Text:
- Authors: Adefisoye, Martins Ajibade
- Date: 2016
- Subjects: Escherichia coli -- South Africa -- Eastern Cape Water -- Purification -- South Africa -- Eastern Cape Effluent quality -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10353/3165 , vital:28328
- Description: South Africa is currently experiencing water shortage crisis, a challenge that has been attributed not only to the scarcity of freshwater, but also to fast degrading water quality. Factors such as rapid urbanisation, population and economic growth, climate change as well as poor operational and maintenance of many of the exisiting water/wastewater treatment facilities have been acknowledged as important contributors to degrading water quality in the country. Untreated or inadequately treated discharged wastewater effluents constitute point source pollution to many freshwater environments in South Africa. Hence, it becomes imperative to evaluate wastewater discharges in other to protect the scarce freshwater resource, the environment and public health. Over a twelve-month sampling period (September 2012 to August 2013), we assessed the bacteriological, virological and physicochemical qualities of the discharged final effluents of two wastewater treatment facilities in the Eastern Cape Province of South Africa. For the physicochemical assessment, a total of 144 final effluent samples were collected from both the final effluent tanks (FE) and the discharge points (DP) of the treatment facilities. Physicochemical parameters including pH, temperature, turbidity, total dissolved solids (TDS), dissolved oxygen (DO), electrical conductivity (EC) and free chlorine concentration were determined on site while biological oxygen demand (BOD), nitrate (NO3-), nitrite (NO2-), phosphate (PO4-) and chemical oxygen demand (COD) were determined in the laboratory. The bacteriological analysis of the samples was done using standard membrane filtration (MF) technique. Bacterial group assessed included: faecal indicator bacteria (faecal coliforms and E. coli) and Vibrio species, while the antibiotic susceptibility profiles of selected E. coli and Vibrio species isolates against some selected antibiotics commonly used in human therapy and veterinary medicine were determind using the standard agar-disc diffusion method. The occurrence and concentrations of human enteric viruses including: human adenovirus (HAdV), hepatitis A virus (HAV) and rotavirus (RoV) in the samples were determined by TaqMan-based real-time polymerase chain reaction (qPCR) following concentration by adsorption-elution method. The physicochemical characteristics of the samples ranged as follows: pH (6.5 – 7.6), TDS (95 – 171 mg/L), EC (134 – 267 μS/cm), temperature (12 – 27 °C), turbidity (1.5 – 65.7 mg/L), free chlorine (0.08 – 0.72 mg/L), DO (2.06 – 9.81 mg/L), BOD (0.13 – 9.81 mg/L), NO3- (0 – 21.5 mg/L), NO2- (0 – 0.72 mg/L), PO4- (0 – 18.3 mg/L) and COD (27 – 680 mg/L). Some of the characteristic such as pH, TDS, EC, temperature, nitrite and DO (on most instances) complied with recommended guidelines. Other characteristics, however, including turbidity, BOD, nitrate, phosphate and COD fell short of the recommended guidelines. All the 48 samples analysed for bacteriological qualities tested positive for the presence of the bacterial groups with significant (P≤0.05) seasonal variation in their densities. Faecal coliforms were detected in counts ranging from 1 CFU/100ml to 2.7 × 104 CFU/100ml. Presumptive E. coli counts ranged generally between 1 CFU/100ml – 1.4 × 105 CFU/100ml while counts of presumptive Vibrio species ranged between 4 CFU/100ml – 1.4 × 104 CFU/100ml. Molecular identification of the presumptive isolates by polymerase chain reactions PCR gave positive reaction rates of 76.2 percent (381/500) and 69.8 percent (279/400) for E.coli and Vibrio species respectively. The antibiotic susceptibility profiling of 205 PCR-confirmed Vibiro isolates against 18 commomly used antibiotics showed resistance frequencies ranging from 0.5 percent (imipenem) to 96.1 percent (penicillin G) at recommended breakpoint concentrations. Eighty-one percent (166/205) of the Vibrio isolates showed multidrug resistance (resistance to 3 or more antibiotics) with the most common multiple antibiotic resistance phenotype (MARP) being AP-T-TM-SMX-PG-NI-PB, occurring in 8 isolates.
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Evaluation of the medicinal potentials of Bulbine Abyssinica A. rich in the management of diabetes mellitus in the Eastern Cape, South Africa
- Authors: Kibiti, Cromwell Mwiti
- Date: 2016
- Subjects: Medicinal plants -- South Africa -- Eastern Cape Diabetes -- Alternative treatment -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10353/3077 , vital:28252
- Description: Diabetes mellitus is a chronic physiological carbohydrate metabolic disorder with significant impact on the economy, quality of life and life expectancy in South Africa. Herbal medicine has become the alternative therapy in the management of this disease. However, their safety and effectiveness have not been investigated. To address this, one of the plants used in Eastern Cape Province, South Africa, Bulbine abyssinica A. Rich (Asphodelaceae), was evaluated. Bulbine abyssinica is one of the species used in the management of diabetes mellitus. This plant was mentioned during an ethnobotanical survey conducted in Nkonkobe municipality of the Eastern Cape Province. Though a decoction prepared from the whole plant is used in the treatment of diabetes mellitus, the mechanism of action and its safety has not been elucidated. Thus, this research work was designed to contribute to the understanding of the possible mechanism of action of B. abyssinica as an antidiabetic medicinal plant and its toxic potentials to the users. The aqueous extract exhibited remarkable inhibitory activity onα-amylase (estimated inhibitory concentration (IC)50 value of 3.28 μg/ml), while the acetone extract exhibited weak inhibitory activity. The acetone extract exhibited notable α-glucosidase inhibitory activity (IC50 value of 4.27 μg/ml) while aqueous extract had significantly weak activity. The Lineweaver-Burk double reciprocal plots revealed that the aqueous extract exerts noncompetitive inhibition on the α-amylase activity while the acetone extract exerts a near competitive inhibitory pattern on the α-glucosidase activity. The extracts from the plant possessed high free radical scavenging activities, with acetone extract exhibiting the highest activities in all assay models used except with ferric reducing power and nitric oxide (NO) scavenging ability. The aqueous extract exhibited the highest ferric reducing power and nitric oxide radical mopping strength while the essential oil exhibited the highest scavenging activities with 1,1-diphenyl-2-picrylhydrazyl (DPPH) and relatively high ferric reducing power and nitric oxide scavenging ability. The acetone extract and the essential oil of this species exhibited higher albumin denaturation inhibition than the aqueous extract while the latter showed the greatest membrane lysis protection. The essential oil, acetone and aqueous extracts from this plant significantly inhibited the growth of Shigelle flexneri, Pseudomonas aeruginosa, Staphylococcus aureus and Enterococcus faecalis. Klebsiella pneumonia, Proteus vulgaris and Streptococcus pyogens growth were inhibited by acetone and aqueous extracts. The essential oil also showed inhibitory activity against Proteus vulgaris. However, the extracts were active against the growth of only three fungi species. The essential oil showed significant inhibitory activity against Trichophyton rubrum. The aqueous extract inhibited the growth of Microsporum gypseum while the acetone extract was active against Microsporum canis, and Microsporum gypseum. The carbohydrate, crude fibre, moisture, ash, crude protein and crude fat of approximately 74.8 percent, 8.9 percent, 8.8 percent, 8 percent, 7.7 percent and 0.6 percent, respectively, were detected in this plant. The species is characterized by moderate levels of oxalates, phytic acids, Vitamin A, Vitamin C and Vitamin E. Potassium and calcium were present in highest levels, while magnesium, iron, sodium, aluminium and phosphorus were moderately present. Manganese, zinc and copper where in low amounts. These vitamins and mineral elements were within their recommended daily allowance (RDA) in humans. The investigation also revealed appreciable amounts of total phenols, flavonoids, flavanols, proanthocyanidins and alkaloids in both acetone and aqueous extracts while saponins and tannins were in trace amounts. The essential oil was characterized by large quantities of terpenes (91.9 percent) and small fraction of esters (8.01 percent).
- Full Text:
- Authors: Kibiti, Cromwell Mwiti
- Date: 2016
- Subjects: Medicinal plants -- South Africa -- Eastern Cape Diabetes -- Alternative treatment -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10353/3077 , vital:28252
- Description: Diabetes mellitus is a chronic physiological carbohydrate metabolic disorder with significant impact on the economy, quality of life and life expectancy in South Africa. Herbal medicine has become the alternative therapy in the management of this disease. However, their safety and effectiveness have not been investigated. To address this, one of the plants used in Eastern Cape Province, South Africa, Bulbine abyssinica A. Rich (Asphodelaceae), was evaluated. Bulbine abyssinica is one of the species used in the management of diabetes mellitus. This plant was mentioned during an ethnobotanical survey conducted in Nkonkobe municipality of the Eastern Cape Province. Though a decoction prepared from the whole plant is used in the treatment of diabetes mellitus, the mechanism of action and its safety has not been elucidated. Thus, this research work was designed to contribute to the understanding of the possible mechanism of action of B. abyssinica as an antidiabetic medicinal plant and its toxic potentials to the users. The aqueous extract exhibited remarkable inhibitory activity onα-amylase (estimated inhibitory concentration (IC)50 value of 3.28 μg/ml), while the acetone extract exhibited weak inhibitory activity. The acetone extract exhibited notable α-glucosidase inhibitory activity (IC50 value of 4.27 μg/ml) while aqueous extract had significantly weak activity. The Lineweaver-Burk double reciprocal plots revealed that the aqueous extract exerts noncompetitive inhibition on the α-amylase activity while the acetone extract exerts a near competitive inhibitory pattern on the α-glucosidase activity. The extracts from the plant possessed high free radical scavenging activities, with acetone extract exhibiting the highest activities in all assay models used except with ferric reducing power and nitric oxide (NO) scavenging ability. The aqueous extract exhibited the highest ferric reducing power and nitric oxide radical mopping strength while the essential oil exhibited the highest scavenging activities with 1,1-diphenyl-2-picrylhydrazyl (DPPH) and relatively high ferric reducing power and nitric oxide scavenging ability. The acetone extract and the essential oil of this species exhibited higher albumin denaturation inhibition than the aqueous extract while the latter showed the greatest membrane lysis protection. The essential oil, acetone and aqueous extracts from this plant significantly inhibited the growth of Shigelle flexneri, Pseudomonas aeruginosa, Staphylococcus aureus and Enterococcus faecalis. Klebsiella pneumonia, Proteus vulgaris and Streptococcus pyogens growth were inhibited by acetone and aqueous extracts. The essential oil also showed inhibitory activity against Proteus vulgaris. However, the extracts were active against the growth of only three fungi species. The essential oil showed significant inhibitory activity against Trichophyton rubrum. The aqueous extract inhibited the growth of Microsporum gypseum while the acetone extract was active against Microsporum canis, and Microsporum gypseum. The carbohydrate, crude fibre, moisture, ash, crude protein and crude fat of approximately 74.8 percent, 8.9 percent, 8.8 percent, 8 percent, 7.7 percent and 0.6 percent, respectively, were detected in this plant. The species is characterized by moderate levels of oxalates, phytic acids, Vitamin A, Vitamin C and Vitamin E. Potassium and calcium were present in highest levels, while magnesium, iron, sodium, aluminium and phosphorus were moderately present. Manganese, zinc and copper where in low amounts. These vitamins and mineral elements were within their recommended daily allowance (RDA) in humans. The investigation also revealed appreciable amounts of total phenols, flavonoids, flavanols, proanthocyanidins and alkaloids in both acetone and aqueous extracts while saponins and tannins were in trace amounts. The essential oil was characterized by large quantities of terpenes (91.9 percent) and small fraction of esters (8.01 percent).
- Full Text:
Examining the nature and extent to which learners with special educational needs are included in regular schools: the case of four primary schools in Cape Town, South Africa
- Authors: Shadaya, Girlie
- Date: 2016
- Subjects: Special education Inclusive education -- South Africa -- Cape Town Children with disabilities -- Education -- South Africa -- Cape Town
- Language: English
- Type: Thesis , Doctoral , Degree
- Identifier: http://hdl.handle.net/10353/5346 , vital:29214
- Description: The study is premised on the assumptions that learners with special educational needs are not fully included in regular schools and that perceptions of teachers influence their behaviour toward and acceptance of learners with special educational needs in regular classes. In light of this, the aim of the current study was to examine the nature and extent to which learners with special educational needs are included in regular schools with the ultimate aim of assisting learners with special educational needs to be fully catered for by schools and teachers. The researcher opted for the mixed method approach which is embedded in the post positivist research paradigm. The mixed method approach makes use of quantitative and qualitative methods of data collection, presentation and analysis. Data were generated from a sample of 60 teachers and 4 principals from 4 regular primary schools mainly through questionnaires. Data were also generated from interviews, observation and documents. These data from interviews, observation and documents were used to buttress results from the questionnaires. The findings of this present study showed that many schools are now moving towards inclusivity. There is a relative prevalence of learners with disability in schools. The study also established that the inclusion of learners with special educational needs in regular schools was faced with a number of problems. There were inadequate professionally-trained teachers in schools. Shortage of classrooms, large class sizes, equipment and materials affected the quality of access to education for learners with special educational needs. Although there was significant support at school level, it emerged that there was inadequate quality in-service training programmes for teachers conducted by district officials. Overall, the findings of the study have confirmed the assumptions of the study. For learners with special educational needs to be fully included in regular schools, the study would recommend that the government improve the quality of teachers through in-service training programmes. Moreover, schools must be adequately resourced and government should commit itself to the alleviation of large class sizes. The study further revealed that, gaps still exist in the inclusion of learners with special educational needs between the intended and the actual practice. The study, therefore, recommends that research be conducted with the possibility of establishing strategies for the inclusion of learners with special educational needs in regular schools. This might improve the actual practice of including learners with iii special educational needs in regular schools. In turn, learners with special educational needs can be said to have equal access to education.
- Full Text:
- Authors: Shadaya, Girlie
- Date: 2016
- Subjects: Special education Inclusive education -- South Africa -- Cape Town Children with disabilities -- Education -- South Africa -- Cape Town
- Language: English
- Type: Thesis , Doctoral , Degree
- Identifier: http://hdl.handle.net/10353/5346 , vital:29214
- Description: The study is premised on the assumptions that learners with special educational needs are not fully included in regular schools and that perceptions of teachers influence their behaviour toward and acceptance of learners with special educational needs in regular classes. In light of this, the aim of the current study was to examine the nature and extent to which learners with special educational needs are included in regular schools with the ultimate aim of assisting learners with special educational needs to be fully catered for by schools and teachers. The researcher opted for the mixed method approach which is embedded in the post positivist research paradigm. The mixed method approach makes use of quantitative and qualitative methods of data collection, presentation and analysis. Data were generated from a sample of 60 teachers and 4 principals from 4 regular primary schools mainly through questionnaires. Data were also generated from interviews, observation and documents. These data from interviews, observation and documents were used to buttress results from the questionnaires. The findings of this present study showed that many schools are now moving towards inclusivity. There is a relative prevalence of learners with disability in schools. The study also established that the inclusion of learners with special educational needs in regular schools was faced with a number of problems. There were inadequate professionally-trained teachers in schools. Shortage of classrooms, large class sizes, equipment and materials affected the quality of access to education for learners with special educational needs. Although there was significant support at school level, it emerged that there was inadequate quality in-service training programmes for teachers conducted by district officials. Overall, the findings of the study have confirmed the assumptions of the study. For learners with special educational needs to be fully included in regular schools, the study would recommend that the government improve the quality of teachers through in-service training programmes. Moreover, schools must be adequately resourced and government should commit itself to the alleviation of large class sizes. The study further revealed that, gaps still exist in the inclusion of learners with special educational needs between the intended and the actual practice. The study, therefore, recommends that research be conducted with the possibility of establishing strategies for the inclusion of learners with special educational needs in regular schools. This might improve the actual practice of including learners with iii special educational needs in regular schools. In turn, learners with special educational needs can be said to have equal access to education.
- Full Text:
Examining the nature and extent to which learners with special educational needs are included in regular schools: the case of four primary schools in Cape Town, South Africa
- Authors: Shadaya, Girlie
- Date: 2016
- Subjects: Special education -- South Africa -- Cape Town Inclusive education -- South Africa -- Cape Town Children with disabilities -- Education -- South Africa -- Cape Town
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10353/2336 , vital:27754
- Description: The study is premised on the assumptions that learners with special educational needs are not fully included in regular schools and that perceptions of teachers influence their behaviour toward and acceptance of learners with special educational needs in regular classes. In light of this, the aim of the current study was to examine the nature and extent to which learners with special educational needs are included in regular schools with the ultimate aim of assisting learners with special educational needs to be fully catered for by schools and teachers. The researcher opted for the mixed method approach which is embedded in the post positivist research paradigm. The mixed method approach makes use of quantitative and qualitative methods of data collection, presentation and analysis. Data were generated from a sample of 60 teachers and 4 principals from 4 regular primary schools mainly through questionnaires. Data were also generated from interviews, observation and documents. These data from interviews, observation and documents were used to buttress results from the questionnaires. The findings of this present study showed that many schools are now moving towards inclusivity. There is a relative prevalence of learners with disability in schools. The study also established that the inclusion of learners with special educational needs in regular schools was faced with a number of problems. There were inadequate professionally-trained teachers in schools. Shortage of classrooms, large class sizes, equipment and materials affected the quality of access to education for learners with special educational needs. Although there was significant support at school level, it emerged that there was inadequate quality in-service training programmes for teachers conducted by district officials. Overall, the findings of the study have confirmed the assumptions of the study. For learners with special educational needs to be fully included in regular schools, the study would recommend that the government improve the quality of teachers through in-service training programmes. Moreover, schools must be adequately resourced and government should commit itself to the alleviation of large class sizes. The study further revealed that, gaps still exist in the inclusion of learners with special educational needs between the intended and the actual practice. The study, therefore, recommends that research be conducted with the possibility of establishing strategies for the inclusion of learners with special educational needs in regular schools. This might improve the actual practice of including learners with special educational needs in regular schools. In turn, learners with special educational needs can be said to have equal access to education.
- Full Text:
- Authors: Shadaya, Girlie
- Date: 2016
- Subjects: Special education -- South Africa -- Cape Town Inclusive education -- South Africa -- Cape Town Children with disabilities -- Education -- South Africa -- Cape Town
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10353/2336 , vital:27754
- Description: The study is premised on the assumptions that learners with special educational needs are not fully included in regular schools and that perceptions of teachers influence their behaviour toward and acceptance of learners with special educational needs in regular classes. In light of this, the aim of the current study was to examine the nature and extent to which learners with special educational needs are included in regular schools with the ultimate aim of assisting learners with special educational needs to be fully catered for by schools and teachers. The researcher opted for the mixed method approach which is embedded in the post positivist research paradigm. The mixed method approach makes use of quantitative and qualitative methods of data collection, presentation and analysis. Data were generated from a sample of 60 teachers and 4 principals from 4 regular primary schools mainly through questionnaires. Data were also generated from interviews, observation and documents. These data from interviews, observation and documents were used to buttress results from the questionnaires. The findings of this present study showed that many schools are now moving towards inclusivity. There is a relative prevalence of learners with disability in schools. The study also established that the inclusion of learners with special educational needs in regular schools was faced with a number of problems. There were inadequate professionally-trained teachers in schools. Shortage of classrooms, large class sizes, equipment and materials affected the quality of access to education for learners with special educational needs. Although there was significant support at school level, it emerged that there was inadequate quality in-service training programmes for teachers conducted by district officials. Overall, the findings of the study have confirmed the assumptions of the study. For learners with special educational needs to be fully included in regular schools, the study would recommend that the government improve the quality of teachers through in-service training programmes. Moreover, schools must be adequately resourced and government should commit itself to the alleviation of large class sizes. The study further revealed that, gaps still exist in the inclusion of learners with special educational needs between the intended and the actual practice. The study, therefore, recommends that research be conducted with the possibility of establishing strategies for the inclusion of learners with special educational needs in regular schools. This might improve the actual practice of including learners with special educational needs in regular schools. In turn, learners with special educational needs can be said to have equal access to education.
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Expand public works program on infrastructure : lessons from international public works programs 1920'S - 1950'S and 2004-2016
- Authors: Zuma, Siziwe Monica
- Date: 2016
- Subjects: Public works--South Africa Labor--South Africa Job creation--South Africa
- Language: English
- Type: Thesis , Doctoral , Public Administration
- Identifier: http://hdl.handle.net/10353/16102 , vital:40664
- Description: The Expanded Public Works Program on Infrastructure is a national program by the South African Government. It is intending to build and maintain public assets using labour intensive mechanisms. It seeks to offer short term employment and training to the labour participants so that when they exit they may be able to find employment. The program also has an economic sector program of the infrastructure sector called Vuk‘uphile for development of emerging contractors also it is a national program. The Expanded Public Works Program is the government main instrument to job creation in South Africa. Government also seeks to pursue policies that are pro-growth, with the hope that the economic growth will absorbed the unemployed into employment. It is also looking into the small business sector to be the main driver for job creation. Despite this the government‘s commitment, the unemployment rate and poverty rate are increasing and the economy is declining. The study sought to examine if the skills obtained by the participants in the Expanded Public Works Program enable the participants to obtain employment upon exiting the program. It also sought to find any challenges the participants encounter before and after living the program. It sought to look at other international public works programs and theories on public works to learn any lessons. I also sought to study if the program cannot be expanded into for it to create more sustainable employment for the enterprises and the labour participants in order to lengthen the period of work experience skills training, reduce the incidents of poverty and stimulate economic growth. This study followed a literature review methodology, using second source data from various literatures, empirical and non-empirical second source literature. The findings indicate that the skills and the duration of the training are not sufficient for the labour participants to be absorbed by the labour force. The short duration of working in the program is too short to make a meaningful income and work experience that can be sustainable. Upon exiting the program they cannot find employment a large majority of them. The program offered in the Zibambele in KwaZulu Natal offers work on a part time basis for the maintenance of roads over a longer period that acts as a safety net. The program is only in KwaZulu Natal and only on road maintenance; it is not big enough to make a meaningful difference to the amount of unemployment in the country. The Vuk‘uphile program however is offering good skills to the emerging contractor that enables their companies to grow. The international public works programs have been instrumental in creating employment to millions of participants and reduced incidents of poverty. The expanded public works program on infrastructure can be expanded to include asset management, maintenance and can expand to rural infrastructure and development and agricultural infrastructure. The study recommends that the government considers expanding the expanded public works program on infrastructure so that it can provide longer and more sustainable employment to labor participants and entrepreneurs. Also that it considers using the model used to develop emerging contractors in the Vuk‘uphile program to develop small medium businesses in the infrastructure and other areas.
- Full Text:
- Authors: Zuma, Siziwe Monica
- Date: 2016
- Subjects: Public works--South Africa Labor--South Africa Job creation--South Africa
- Language: English
- Type: Thesis , Doctoral , Public Administration
- Identifier: http://hdl.handle.net/10353/16102 , vital:40664
- Description: The Expanded Public Works Program on Infrastructure is a national program by the South African Government. It is intending to build and maintain public assets using labour intensive mechanisms. It seeks to offer short term employment and training to the labour participants so that when they exit they may be able to find employment. The program also has an economic sector program of the infrastructure sector called Vuk‘uphile for development of emerging contractors also it is a national program. The Expanded Public Works Program is the government main instrument to job creation in South Africa. Government also seeks to pursue policies that are pro-growth, with the hope that the economic growth will absorbed the unemployed into employment. It is also looking into the small business sector to be the main driver for job creation. Despite this the government‘s commitment, the unemployment rate and poverty rate are increasing and the economy is declining. The study sought to examine if the skills obtained by the participants in the Expanded Public Works Program enable the participants to obtain employment upon exiting the program. It also sought to find any challenges the participants encounter before and after living the program. It sought to look at other international public works programs and theories on public works to learn any lessons. I also sought to study if the program cannot be expanded into for it to create more sustainable employment for the enterprises and the labour participants in order to lengthen the period of work experience skills training, reduce the incidents of poverty and stimulate economic growth. This study followed a literature review methodology, using second source data from various literatures, empirical and non-empirical second source literature. The findings indicate that the skills and the duration of the training are not sufficient for the labour participants to be absorbed by the labour force. The short duration of working in the program is too short to make a meaningful income and work experience that can be sustainable. Upon exiting the program they cannot find employment a large majority of them. The program offered in the Zibambele in KwaZulu Natal offers work on a part time basis for the maintenance of roads over a longer period that acts as a safety net. The program is only in KwaZulu Natal and only on road maintenance; it is not big enough to make a meaningful difference to the amount of unemployment in the country. The Vuk‘uphile program however is offering good skills to the emerging contractor that enables their companies to grow. The international public works programs have been instrumental in creating employment to millions of participants and reduced incidents of poverty. The expanded public works program on infrastructure can be expanded to include asset management, maintenance and can expand to rural infrastructure and development and agricultural infrastructure. The study recommends that the government considers expanding the expanded public works program on infrastructure so that it can provide longer and more sustainable employment to labor participants and entrepreneurs. Also that it considers using the model used to develop emerging contractors in the Vuk‘uphile program to develop small medium businesses in the infrastructure and other areas.
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Exploration of Nahoon beach milieu for lignocellulose degrading bacteria and optimizing fermentation conditions for holocellulase production by selected strains
- Authors: Fatokun, Evelyn
- Date: 2016
- Subjects: Lignocellulose Lignocellulose -- Biodegradation Water -- Purification -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10353/1529 , vital:27413
- Description: A significant trend in the modern day industrial biotechnology is the utilization and application of renewable resources, and ecofriendly approach to industrial processes and waste management. As a consequence thereof, the biotechnology of holocellulases: cellulase and xylanase and, enzymatic hydrolysis of renewable and abundant lignocellulosic biomass to energy and value added products are rapidly increasing; hence, cost effective enzyme system is imperative. In that context, exploration of microbiota for strains and enzymes with novel industrial properties is vital for efficient and commercially viable enzyme biotechnology. Consequent on the complex characteristics of high salinity, variable pressure, temperature and nutritional conditions, bacterial strains from the marine environment are equipped with enzyme machinery of industrial importance for adaptation and survival. In this study, bacterial strains were isolated form Nahoon beach and optimized for holocellulase production. Three isolates selected for lignocellulolytic potential were identified by 16S ribosomal deoxyribonucleic acid (rDNA) sequence analysis. Isolate FS1k had 98 percent similarity with Streptomyces albidoflavus strain AIH12, was designated as Streptomyces albidoflavus strain SAMRC-UFH5 and deposited in the GenBank with accession number KU171373. Similarly, isolates CS14b and CS22d with respective percentage similarity of 98 and 99 (percent) with Bacillus cereus strains and Streptomyces sp. strain WMMB251 were named Bacillus cereus strain SAMRC-UFH9 and Streptomyces sp. strain SAMRC-UFH6; and were deposited in the GenBank with accession number KX524510 and KU171374 respectively. Optimal pH, temperature and agitation speed for cellulase production by S. albidoflavus strain SAMRC-UFH5, and B. cereus strain SAMRC-UFH9 were 6 and 7; 40 and 30 (°C); and 100 and 150 (rpm) respectively; while xylanase production was optimal at pH, temperature and agitation speed of 8 and 7; 40 and 30 (°C); and 150 and 50 (rpm) respectively. Maximum cellulase activity of 0.26 and 0.061(U/mL) by S. albidoflavus strain SAMRC-UFH5 and B. cereus strain SAMRC-UFH9 were attained at 60 h respectively, while maximal xylanase activity of 18.54 and 16.6 (U/mL) was produced by S. albidoflavus strain SAMRC-UFH5 and B. cereus strain SAMRC-UFH9 at 48 h and 60 h respectively. Furthermore, xylanase production by S. albidoflavus strain SAMRC-UFH5 and B. cereus strain SAMRC-UFH9 was maximally induced by wheat straw and xylan respectively, while cellulase production was best induced by mannose and carboxymethyl cellulose respectively. On the other hand, cellulase and xylanase production by Streptomyces sp. strain SAMRC-UFH6 was optimal at pH, temperature and agitation speed of 7 and 8, 40 °C and 100 rpm, respectively. Highest production of cellulase and xylanase was attained at 84 and 60 h with respective activity of 0.065 and 6.34 (U/mL). In addition, cellulase and xylanase production by the strain was best induced by beechwood xylan. Moreover, xylanase produced by Streptomyces sp. strain SAMRC-UFH6 at optimal conditions was characterized by optimal pH and temperature of 8 and 80-90 °C respectively; retaining over 70 percent activity at pH 5-10 after 1 h and 60 percent of initial activity at 90 °C after 90 min of incubation. In all, optimization improved cellulase and xylanase production yields, being 40 and 95.5, 10.89 and 72.17, and 10 and 115- fold increase by S. albidoflavus strain SAMRC-UFH5, B. cereus strain SAMRC-UFH9 and Streptomyces sp. SAMRC-UFH6 respectively. The results of this study suggest that the marine bacterial strains are resource for holocellulase with industrial applications.
- Full Text:
- Authors: Fatokun, Evelyn
- Date: 2016
- Subjects: Lignocellulose Lignocellulose -- Biodegradation Water -- Purification -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10353/1529 , vital:27413
- Description: A significant trend in the modern day industrial biotechnology is the utilization and application of renewable resources, and ecofriendly approach to industrial processes and waste management. As a consequence thereof, the biotechnology of holocellulases: cellulase and xylanase and, enzymatic hydrolysis of renewable and abundant lignocellulosic biomass to energy and value added products are rapidly increasing; hence, cost effective enzyme system is imperative. In that context, exploration of microbiota for strains and enzymes with novel industrial properties is vital for efficient and commercially viable enzyme biotechnology. Consequent on the complex characteristics of high salinity, variable pressure, temperature and nutritional conditions, bacterial strains from the marine environment are equipped with enzyme machinery of industrial importance for adaptation and survival. In this study, bacterial strains were isolated form Nahoon beach and optimized for holocellulase production. Three isolates selected for lignocellulolytic potential were identified by 16S ribosomal deoxyribonucleic acid (rDNA) sequence analysis. Isolate FS1k had 98 percent similarity with Streptomyces albidoflavus strain AIH12, was designated as Streptomyces albidoflavus strain SAMRC-UFH5 and deposited in the GenBank with accession number KU171373. Similarly, isolates CS14b and CS22d with respective percentage similarity of 98 and 99 (percent) with Bacillus cereus strains and Streptomyces sp. strain WMMB251 were named Bacillus cereus strain SAMRC-UFH9 and Streptomyces sp. strain SAMRC-UFH6; and were deposited in the GenBank with accession number KX524510 and KU171374 respectively. Optimal pH, temperature and agitation speed for cellulase production by S. albidoflavus strain SAMRC-UFH5, and B. cereus strain SAMRC-UFH9 were 6 and 7; 40 and 30 (°C); and 100 and 150 (rpm) respectively; while xylanase production was optimal at pH, temperature and agitation speed of 8 and 7; 40 and 30 (°C); and 150 and 50 (rpm) respectively. Maximum cellulase activity of 0.26 and 0.061(U/mL) by S. albidoflavus strain SAMRC-UFH5 and B. cereus strain SAMRC-UFH9 were attained at 60 h respectively, while maximal xylanase activity of 18.54 and 16.6 (U/mL) was produced by S. albidoflavus strain SAMRC-UFH5 and B. cereus strain SAMRC-UFH9 at 48 h and 60 h respectively. Furthermore, xylanase production by S. albidoflavus strain SAMRC-UFH5 and B. cereus strain SAMRC-UFH9 was maximally induced by wheat straw and xylan respectively, while cellulase production was best induced by mannose and carboxymethyl cellulose respectively. On the other hand, cellulase and xylanase production by Streptomyces sp. strain SAMRC-UFH6 was optimal at pH, temperature and agitation speed of 7 and 8, 40 °C and 100 rpm, respectively. Highest production of cellulase and xylanase was attained at 84 and 60 h with respective activity of 0.065 and 6.34 (U/mL). In addition, cellulase and xylanase production by the strain was best induced by beechwood xylan. Moreover, xylanase produced by Streptomyces sp. strain SAMRC-UFH6 at optimal conditions was characterized by optimal pH and temperature of 8 and 80-90 °C respectively; retaining over 70 percent activity at pH 5-10 after 1 h and 60 percent of initial activity at 90 °C after 90 min of incubation. In all, optimization improved cellulase and xylanase production yields, being 40 and 95.5, 10.89 and 72.17, and 10 and 115- fold increase by S. albidoflavus strain SAMRC-UFH5, B. cereus strain SAMRC-UFH9 and Streptomyces sp. SAMRC-UFH6 respectively. The results of this study suggest that the marine bacterial strains are resource for holocellulase with industrial applications.
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Exploring the use and relevance of narrative approaches to psycho-social interventions in the south African context : a mixed methods content analysis
- Authors: Smit, Christelle
- Date: 2016
- Subjects: Narrative therapy
- Language: English
- Type: Thesis , Masters , MSoc
- Identifier: http://hdl.handle.net/10353/6432 , vital:29684
- Description: This study aims to explore the use and relevance of Narrative approaches to psycho-social interventions in the South African context. The profession of psychology in South Africa has been in a state of discontentment since the country’s turn to democracy in 1994 which has been voiced from both those within the profession and those it aims to assist. The loudest call is for a psychology that is relevant to the South African context – culturally, socially, and politically. Narrative approaches to psychotherapy and psycho-social intervention are grounded in post-modern and social-constructionist thought and offer an alternative to mainstream psychological theory. Narrative practice aims to promote social justice and views therapy as a political act. It is also an approach that values local knowledges and sees all therapeutic engagements as cross-cultural encounters which are approached with curiosity and a not-knowing stance, rather than an interpretive, analytical lens. This study has investigated what the existing literature has produced regarding the use and relevance of Narrative approaches in South Africa context. The research process was implemented using a mixed methods research methodology whereby a sample of 58 journal articles (n=58) were analysed using both quantitative and qualitative content analysis. The common themes that emerged from the articles were ‘viewing people in context’, ‘listening to the telling of stories’, ‘theoretical constructs of a narrative approach’, and ‘social phenomena’.
- Full Text:
- Authors: Smit, Christelle
- Date: 2016
- Subjects: Narrative therapy
- Language: English
- Type: Thesis , Masters , MSoc
- Identifier: http://hdl.handle.net/10353/6432 , vital:29684
- Description: This study aims to explore the use and relevance of Narrative approaches to psycho-social interventions in the South African context. The profession of psychology in South Africa has been in a state of discontentment since the country’s turn to democracy in 1994 which has been voiced from both those within the profession and those it aims to assist. The loudest call is for a psychology that is relevant to the South African context – culturally, socially, and politically. Narrative approaches to psychotherapy and psycho-social intervention are grounded in post-modern and social-constructionist thought and offer an alternative to mainstream psychological theory. Narrative practice aims to promote social justice and views therapy as a political act. It is also an approach that values local knowledges and sees all therapeutic engagements as cross-cultural encounters which are approached with curiosity and a not-knowing stance, rather than an interpretive, analytical lens. This study has investigated what the existing literature has produced regarding the use and relevance of Narrative approaches in South Africa context. The research process was implemented using a mixed methods research methodology whereby a sample of 58 journal articles (n=58) were analysed using both quantitative and qualitative content analysis. The common themes that emerged from the articles were ‘viewing people in context’, ‘listening to the telling of stories’, ‘theoretical constructs of a narrative approach’, and ‘social phenomena’.
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