Incidence of Staphylococcus species in bovine milk: their antimicrobial sensitivity in selected antibiotics and Usnea barbata lichen extracts
- Idamokoro, Emrobowansan Monday
- Authors: Idamokoro, Emrobowansan Monday
- Date: 2013
- Subjects: Staphylococcus , Microbial sensitivity tests , Traditional medicine , Lichens -- Analysis , Mastitis , Antibiotics in agriculture
- Language: English
- Type: Thesis , Masters , MSc Agric (Animal Science)
- Identifier: vital:11829 , http://hdl.handle.net/10353/d1016193 , Staphylococcus , Microbial sensitivity tests , Traditional medicine , Lichens -- Analysis , Mastitis , Antibiotics in agriculture
- Description: This study was done in order to assess the incidence of Staphylococcus species from milk of cows with subclinical mastitis and their antimicrobial sensitivity in some selected antibiotics and Usnea barbata lichen extracts. The study was conducted in two different commercial dairy farms. Staphylococcus species isolates were identified using several biochemical tests which included Gram’s staining test, catalase test and oxidase test. A commercial API® staph kit (bioMerieux, France) was used to confirm the bacterial organisms to their species level. The antimicrobial sensitivity of individual species was determined according to the Clinical Laboratory Standard Institute (CLSI) for the selected antibiotics. Agar well diffusion method and the broth micro-dilution technique were used to determine the sensitivity of Staphylococcus species in U. barbata extracts. A total of 467 milk samples were screened for bacterial identification from the two farms. Fifteen different Staphylococcus species isolates were identified from all milk samples that were examined. The most frequently isolated species included Staphylococcus xylosus (54.34%), Staphylococcus hominis (24.78%), Staphylococcus aureus (16.38%), Staphylococcus saprophyticus (16.12%) and Staphylococcus haemolyticus (11.63%). Most Staphylococcus species were resistant to Penicillin (75.35%), Nalidixic acid (72.55%) and Ampicillin (63%). Furthermore, the bacterial sensitivity evaluation of U. barbata lichen extracted with methanol and ethyl-acetate against selected Staphylococcus species isolates showed 92.31% and 53.85% susceptibility, respectively. The minimum inhibitory concentration (MIC) of the methanol and ethyl-acetate extracts ranged between 0.0390 to 10 mg/ml. There was a relatively high incidence of Staphylococcus species identified in milk of cows with subclinical mastitis from both farms. Conversely, Staphylococcus species isolates were resistant to antibiotics (mostly penicillin and ampicillin) commonly used in the farms. Furthermore, the study investigated the antimicrobial sensitivity of U. barbata extract in-vitro which may validate its use in traditional medicine for treatment of cows with mastitis.
- Full Text:
- Date Issued: 2013
- Authors: Idamokoro, Emrobowansan Monday
- Date: 2013
- Subjects: Staphylococcus , Microbial sensitivity tests , Traditional medicine , Lichens -- Analysis , Mastitis , Antibiotics in agriculture
- Language: English
- Type: Thesis , Masters , MSc Agric (Animal Science)
- Identifier: vital:11829 , http://hdl.handle.net/10353/d1016193 , Staphylococcus , Microbial sensitivity tests , Traditional medicine , Lichens -- Analysis , Mastitis , Antibiotics in agriculture
- Description: This study was done in order to assess the incidence of Staphylococcus species from milk of cows with subclinical mastitis and their antimicrobial sensitivity in some selected antibiotics and Usnea barbata lichen extracts. The study was conducted in two different commercial dairy farms. Staphylococcus species isolates were identified using several biochemical tests which included Gram’s staining test, catalase test and oxidase test. A commercial API® staph kit (bioMerieux, France) was used to confirm the bacterial organisms to their species level. The antimicrobial sensitivity of individual species was determined according to the Clinical Laboratory Standard Institute (CLSI) for the selected antibiotics. Agar well diffusion method and the broth micro-dilution technique were used to determine the sensitivity of Staphylococcus species in U. barbata extracts. A total of 467 milk samples were screened for bacterial identification from the two farms. Fifteen different Staphylococcus species isolates were identified from all milk samples that were examined. The most frequently isolated species included Staphylococcus xylosus (54.34%), Staphylococcus hominis (24.78%), Staphylococcus aureus (16.38%), Staphylococcus saprophyticus (16.12%) and Staphylococcus haemolyticus (11.63%). Most Staphylococcus species were resistant to Penicillin (75.35%), Nalidixic acid (72.55%) and Ampicillin (63%). Furthermore, the bacterial sensitivity evaluation of U. barbata lichen extracted with methanol and ethyl-acetate against selected Staphylococcus species isolates showed 92.31% and 53.85% susceptibility, respectively. The minimum inhibitory concentration (MIC) of the methanol and ethyl-acetate extracts ranged between 0.0390 to 10 mg/ml. There was a relatively high incidence of Staphylococcus species identified in milk of cows with subclinical mastitis from both farms. Conversely, Staphylococcus species isolates were resistant to antibiotics (mostly penicillin and ampicillin) commonly used in the farms. Furthermore, the study investigated the antimicrobial sensitivity of U. barbata extract in-vitro which may validate its use in traditional medicine for treatment of cows with mastitis.
- Full Text:
- Date Issued: 2013
The effect of moringa oleifera leaf meal on growth perfomance, gut integrity, bone strenght, quality and oxidative stability of meat from broiler chickens
- Authors: Nkukwana, Tobela T
- Date: 2012
- Subjects: Moringa oleifera , Chickens -- Nutrition , Chickens -- Feeding and feeds , Broilers (Chickens)
- Language: English
- Type: Thesis , Doctoral , PhD (Animal Science)
- Identifier: vital:11820 , http://hdl.handle.net/10353/d1006835 , Moringa oleifera , Chickens -- Nutrition , Chickens -- Feeding and feeds , Broilers (Chickens)
- Description: This study was designed was to determine the effects of additive supplementation of Moringa oleifera leaf meal on growth performance, digestibility, digestive organ size, intestinal integrity, bone ash content and bone breaking strength, as well as meat yield and quality of broiler chickens. A total of 2400 day-old unsexed Cobb-500 broiler chicks were randomly allocated to 5 treatment groups: T1, positive control, 668 g/ton Salinomycin and 500 g/ton Albac; T2, T3 and T4 contained graded levels of MOLM at 1 percent, 3 percent and 5 percent of dry matter (DM) intake, respectively; and T5, a negative control (0 percent additives) in a complete randomized design experiment. Except for week one, FI and FCR was highest (P < 0.05) in T4 birds; while T1 birds had the highest FI in the period of 22 to 27d (P < 0.05). Throughout the production period, birds supplemented with MOLM had the highest BW (P < 0.05) than the birds fed the control diets. Feed intake (FI) and feed conversion ratio (FCR) among treatments was highest (P < 0.05) in T4 birds during the period of 8 to 14 d; and was highest (P < 0.05) for T1 birds in the period of 22 to 27d. Protein efficiency ratio (PER) and energy utilization efficiency (EEU) ratios were statistically significant among treatments (P < 0.05). However, dietary treatments had no effect (P > 0.05) on the weights of the heart, liver, spleen, or the gizzard, although the bursa for T2 birds was the lightest (P < 0.05); while gizzard erosion score was highest in T2 birds. All of the nutrients measured, except for fat, had negative intercepts that were significantly different (P < 0.05) from zero, indicating the presence of endogenous fecal losses. Tibiae length (TL) was highest in T2. The dried defatted weight (DW) was heaviest (P < 0.05) for T3 (11.20 ± 0.347) and T5 (11.08 ± 0.328). A positive correlation (r = 0.667; P < 0.01) between TW and DW was observed. There were no dietary effects on bone breaking strength (BBS), but T1 tibiae had highest resistance to breaking force (T1 > T4 > T3 > T2 > T5). Calcium was highest (P < 0.05) in T1; and lowest inT2 and T5. Phosphorus levels were lowest (P < 0.05) in T1; and highest (P < 0.05) in T5 compared. The highest Ca: P ratio was obtained in T4 (P < 0.05); while the ash percent was highest (P < 0.05) in T1. Drip loss increased as L* values increased; and a negative correlation was observed between L* and pH. On D1, C18: 0 and C22 in T2, while C15:0 was highest in T4. On D1, C20:2, C20:3n6 and C22:6n3 were highest in T2 (P < 0.05); T4 had the highest C18:3n6 (P < 0.05), while C20:2 was highest in T5 (P < 0.05). The P/S ratio on D1 was highest in T4; while n-6/n-3 was highest in T1; and n-3 was highest in T3. On D8, the n-3 was highest in T1 (P < 0.05). Results of the current study show that supplementation of M. oleifera leaf at additive levels of up to 5 percent of the bird’s DMI does have the potential to influence the bird performance without any detrimental effects on nutrient utilization, bird health, bone strength and/or meat quality, which can be concluded that MOLM enhanced the bird’s genetic potential for optimal productivity.
- Full Text:
- Date Issued: 2012
- Authors: Nkukwana, Tobela T
- Date: 2012
- Subjects: Moringa oleifera , Chickens -- Nutrition , Chickens -- Feeding and feeds , Broilers (Chickens)
- Language: English
- Type: Thesis , Doctoral , PhD (Animal Science)
- Identifier: vital:11820 , http://hdl.handle.net/10353/d1006835 , Moringa oleifera , Chickens -- Nutrition , Chickens -- Feeding and feeds , Broilers (Chickens)
- Description: This study was designed was to determine the effects of additive supplementation of Moringa oleifera leaf meal on growth performance, digestibility, digestive organ size, intestinal integrity, bone ash content and bone breaking strength, as well as meat yield and quality of broiler chickens. A total of 2400 day-old unsexed Cobb-500 broiler chicks were randomly allocated to 5 treatment groups: T1, positive control, 668 g/ton Salinomycin and 500 g/ton Albac; T2, T3 and T4 contained graded levels of MOLM at 1 percent, 3 percent and 5 percent of dry matter (DM) intake, respectively; and T5, a negative control (0 percent additives) in a complete randomized design experiment. Except for week one, FI and FCR was highest (P < 0.05) in T4 birds; while T1 birds had the highest FI in the period of 22 to 27d (P < 0.05). Throughout the production period, birds supplemented with MOLM had the highest BW (P < 0.05) than the birds fed the control diets. Feed intake (FI) and feed conversion ratio (FCR) among treatments was highest (P < 0.05) in T4 birds during the period of 8 to 14 d; and was highest (P < 0.05) for T1 birds in the period of 22 to 27d. Protein efficiency ratio (PER) and energy utilization efficiency (EEU) ratios were statistically significant among treatments (P < 0.05). However, dietary treatments had no effect (P > 0.05) on the weights of the heart, liver, spleen, or the gizzard, although the bursa for T2 birds was the lightest (P < 0.05); while gizzard erosion score was highest in T2 birds. All of the nutrients measured, except for fat, had negative intercepts that were significantly different (P < 0.05) from zero, indicating the presence of endogenous fecal losses. Tibiae length (TL) was highest in T2. The dried defatted weight (DW) was heaviest (P < 0.05) for T3 (11.20 ± 0.347) and T5 (11.08 ± 0.328). A positive correlation (r = 0.667; P < 0.01) between TW and DW was observed. There were no dietary effects on bone breaking strength (BBS), but T1 tibiae had highest resistance to breaking force (T1 > T4 > T3 > T2 > T5). Calcium was highest (P < 0.05) in T1; and lowest inT2 and T5. Phosphorus levels were lowest (P < 0.05) in T1; and highest (P < 0.05) in T5 compared. The highest Ca: P ratio was obtained in T4 (P < 0.05); while the ash percent was highest (P < 0.05) in T1. Drip loss increased as L* values increased; and a negative correlation was observed between L* and pH. On D1, C18: 0 and C22 in T2, while C15:0 was highest in T4. On D1, C20:2, C20:3n6 and C22:6n3 were highest in T2 (P < 0.05); T4 had the highest C18:3n6 (P < 0.05), while C20:2 was highest in T5 (P < 0.05). The P/S ratio on D1 was highest in T4; while n-6/n-3 was highest in T1; and n-3 was highest in T3. On D8, the n-3 was highest in T1 (P < 0.05). Results of the current study show that supplementation of M. oleifera leaf at additive levels of up to 5 percent of the bird’s DMI does have the potential to influence the bird performance without any detrimental effects on nutrient utilization, bird health, bone strength and/or meat quality, which can be concluded that MOLM enhanced the bird’s genetic potential for optimal productivity.
- Full Text:
- Date Issued: 2012
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