An exploration of leadership attributes for good governance in selected public community learning centres in the Eastern Cape province
- Madikiza-Sambane, Ndiliwe Christabell
- Authors: Madikiza-Sambane, Ndiliwe Christabell
- Date: 2017
- Subjects: Human services -- South Africa -- Eastern Cape Political leadership -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MPA
- Identifier: http://hdl.handle.net/10353/13403 , vital:39658
- Description: The success of any government depends on its leadership’s efficiency and effectiveness in ensuring that government’s vision is realized. It therefore becomes important that all public administration activities are in line with the elements of good governance as reflected on the Constitution of the Republic of South Africa, 1996. These elements include, among others, responsiveness to the needs of the public, accountability, public participation, equity, effectiveness and efficiency. Leadership attributes play a pivotal role in this regard, as all leaders are expected to be able to give guidance and direction towards the vision of government, good communicators, have humble confidence and display exemplary behavior to be successful in their leadership responsibilities. This includes leaders who are assigned responsibilities at all levels and sectors, both in public and private institutions. The purpose of this study therefore, was to explore leadership attributes for good governance in selected Public Community Learning Centres (PCLCs) in the Eastern Cape Province and its focus was in Libode District. It sought to answer a number of questions relating to community participation, policy formulation and implementation, problem solving and the envisaged solutions to address the problems that enhance good governance in the PCLCs. The study is viewed as significant in assisting the process of putting new systems in place for the implementation of new approaches in adult education. The extensive literature review that was conducted before the study resumed contributed tremendously in the success of the study as it provided more insight to the study. The qualitative approach was mainly used and the data was collected through self- administered questionnaires, interviews and observation. The findings of the study revealed challenges that exist with regards to leadership attributes and the enhancement of good governance in Public Community Learning Centres. All participating Ward Councillors (the community leaders) expressed their ignorance about what is happening in the PCLCs. However, they expressed their willingness to work together with the centres for the benefit of all community members within their wards. The recommendations of the study are based on the problems revealed in the findings and provide solutions and answers to the research questions.
- Full Text:
- Authors: Madikiza-Sambane, Ndiliwe Christabell
- Date: 2017
- Subjects: Human services -- South Africa -- Eastern Cape Political leadership -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MPA
- Identifier: http://hdl.handle.net/10353/13403 , vital:39658
- Description: The success of any government depends on its leadership’s efficiency and effectiveness in ensuring that government’s vision is realized. It therefore becomes important that all public administration activities are in line with the elements of good governance as reflected on the Constitution of the Republic of South Africa, 1996. These elements include, among others, responsiveness to the needs of the public, accountability, public participation, equity, effectiveness and efficiency. Leadership attributes play a pivotal role in this regard, as all leaders are expected to be able to give guidance and direction towards the vision of government, good communicators, have humble confidence and display exemplary behavior to be successful in their leadership responsibilities. This includes leaders who are assigned responsibilities at all levels and sectors, both in public and private institutions. The purpose of this study therefore, was to explore leadership attributes for good governance in selected Public Community Learning Centres (PCLCs) in the Eastern Cape Province and its focus was in Libode District. It sought to answer a number of questions relating to community participation, policy formulation and implementation, problem solving and the envisaged solutions to address the problems that enhance good governance in the PCLCs. The study is viewed as significant in assisting the process of putting new systems in place for the implementation of new approaches in adult education. The extensive literature review that was conducted before the study resumed contributed tremendously in the success of the study as it provided more insight to the study. The qualitative approach was mainly used and the data was collected through self- administered questionnaires, interviews and observation. The findings of the study revealed challenges that exist with regards to leadership attributes and the enhancement of good governance in Public Community Learning Centres. All participating Ward Councillors (the community leaders) expressed their ignorance about what is happening in the PCLCs. However, they expressed their willingness to work together with the centres for the benefit of all community members within their wards. The recommendations of the study are based on the problems revealed in the findings and provide solutions and answers to the research questions.
- Full Text:
An exploration of the effectiveness of social work interventions in assisting children living with HIV/AIDS : the case of Nobuhle Unit 8 Community Clinic, Mdantsane, Eastern Cape
- Authors: Mdlankomo, Andiswa Pamella
- Date: 2017
- Subjects: Social case work -- South Africa -- Eastern Cape Social work with children -- South Africa -- Eastern Cape HIV infections
- Language: English
- Type: Thesis , Masters , MSoc
- Identifier: http://hdl.handle.net/10353/8574 , vital:33137
- Description: Social work practice in South African societies is of paramount importance because of high levels of poverty and various vulnerabilities. Despite the fact that there is much research on social work and children living with HIV/AIDS in South Africa, the link between social work approaches and vulnerable children has remained silent. Therefore, it was necessary to explore the quality of service delivery and understand how effective are social work interventions towards specific vulnerable children with the aim of enhancing their wellbeing. Thus, the study explored social work interventions towards assisting children living with HIV/AIDS. The study adopted qualitative research approach method for the purposes of collecting data. In-depth interviews were used as methods of data collection to find out the effectiveness and relevancy of social work interventions towards assisting children living with HIV/AIDS. The data was thematically analysed. The main findings indicated a lack of commitment in social work practice and that mirrors a lack of adequate Continuous Professional Development Training (CPDT); Social work practice in Mdantsane is overwhelmed by statutory work; and social work practice as associated with monotonous duties or an inescapable boring work routine. Moreover, the study unearthed that social workers‟ interventions towards assisting children living with HIV/AIDS are ineffective in Mdantsane Nobuhle Unit 8 community due to service delivery constraints faced by social workers, such as lack of specific HIV/AIDS proper care work training of social workers, lack of resources, high caseloads, misconceptions, and poor implementation of governmental policies. Hence, the study recommends the need for more social workers to fill up this shortage. Thus, the study recommended that there should be an indigenous social welfare and social work practice of assisting children infected and affected by HIV/AIDs. Furthermore, the study recommended that social workers should be equipped with local based skills and knowledge so as to be able to practice professionally. The study concluded that, there should be practical interventions looking into the socio-economic and political contexts and challenging the theorization of the practice, but adopting a more pragmatic approach through influencing and lobbying the government to respondent to the children needs.
- Full Text:
- Authors: Mdlankomo, Andiswa Pamella
- Date: 2017
- Subjects: Social case work -- South Africa -- Eastern Cape Social work with children -- South Africa -- Eastern Cape HIV infections
- Language: English
- Type: Thesis , Masters , MSoc
- Identifier: http://hdl.handle.net/10353/8574 , vital:33137
- Description: Social work practice in South African societies is of paramount importance because of high levels of poverty and various vulnerabilities. Despite the fact that there is much research on social work and children living with HIV/AIDS in South Africa, the link between social work approaches and vulnerable children has remained silent. Therefore, it was necessary to explore the quality of service delivery and understand how effective are social work interventions towards specific vulnerable children with the aim of enhancing their wellbeing. Thus, the study explored social work interventions towards assisting children living with HIV/AIDS. The study adopted qualitative research approach method for the purposes of collecting data. In-depth interviews were used as methods of data collection to find out the effectiveness and relevancy of social work interventions towards assisting children living with HIV/AIDS. The data was thematically analysed. The main findings indicated a lack of commitment in social work practice and that mirrors a lack of adequate Continuous Professional Development Training (CPDT); Social work practice in Mdantsane is overwhelmed by statutory work; and social work practice as associated with monotonous duties or an inescapable boring work routine. Moreover, the study unearthed that social workers‟ interventions towards assisting children living with HIV/AIDS are ineffective in Mdantsane Nobuhle Unit 8 community due to service delivery constraints faced by social workers, such as lack of specific HIV/AIDS proper care work training of social workers, lack of resources, high caseloads, misconceptions, and poor implementation of governmental policies. Hence, the study recommends the need for more social workers to fill up this shortage. Thus, the study recommended that there should be an indigenous social welfare and social work practice of assisting children infected and affected by HIV/AIDs. Furthermore, the study recommended that social workers should be equipped with local based skills and knowledge so as to be able to practice professionally. The study concluded that, there should be practical interventions looking into the socio-economic and political contexts and challenging the theorization of the practice, but adopting a more pragmatic approach through influencing and lobbying the government to respondent to the children needs.
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An Information Security Policy Compliance Reinforcement and Assessment Framework
- Authors: Gundu, Tapiwa
- Date: 2017
- Subjects: Computer security Information technology -- Security measures Business -- Data processing -- Security measures Computer networks -- Security measures
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10353/9556 , vital:34445
- Description: The majority of SMEs have adopted the use of information communication and technology (ICT) services. However, this has exposed their systems to new internal and external security vulnerabilities. These SMEs seem more concerned with external threat related vulnerabilities rather than those from internal threats, although researchers and industry are suggesting a substantial proportion of security incidents to be originating from insiders. Internal threat is often addressed by, firstly, a security policy in order to direct activities and, secondly, organisational information security training and awareness programmes. These two approaches aim to ensure that employees are proficient in their roles and that they know how to carry out their responsibilities securely. There has been a significant amount of research conducted to ensure that information security programmes communicate the information security policy effectively and reinforce sound security practice. However, an assessment of the genuine effectiveness of such programmes is seldom carried out. The purposes of this research study were, firstly, to highlight the flaws in assessing behavioural intentions and equating such behavioural intentions with actual behaviours in information security; secondly, to present an information security policy compliance reinforcement and assessment framework which assists in promoting the conversion of intentions into actual behaviours and in assessing the behavioural change. The approach used was based on the Theory of Planned Behaviour, knowledge, attitude and behaviour theory and Deterrence Theory. Expert review and action research methods were used to validate and refine the framework. The action research was rigorously conducted in four iterations at an SME in South Africa and involved 30 participating employees. The main findings of the study revealed that even though employees may have been well trained and are aware of information security good practice, they may be either unable or unwilling to comply with such practice. The findings of the study also revealed that awareness drives which lead to secure behavioural intents are merely a first step in information security compliance. The study found that not all behavioural intentions converted to actual secure behaviours and only 64% converted. However, deterrence using rewards for good behaviour and punishment for undesirable behaviour was able to increase the conversion by 21%.
- Full Text:
- Authors: Gundu, Tapiwa
- Date: 2017
- Subjects: Computer security Information technology -- Security measures Business -- Data processing -- Security measures Computer networks -- Security measures
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10353/9556 , vital:34445
- Description: The majority of SMEs have adopted the use of information communication and technology (ICT) services. However, this has exposed their systems to new internal and external security vulnerabilities. These SMEs seem more concerned with external threat related vulnerabilities rather than those from internal threats, although researchers and industry are suggesting a substantial proportion of security incidents to be originating from insiders. Internal threat is often addressed by, firstly, a security policy in order to direct activities and, secondly, organisational information security training and awareness programmes. These two approaches aim to ensure that employees are proficient in their roles and that they know how to carry out their responsibilities securely. There has been a significant amount of research conducted to ensure that information security programmes communicate the information security policy effectively and reinforce sound security practice. However, an assessment of the genuine effectiveness of such programmes is seldom carried out. The purposes of this research study were, firstly, to highlight the flaws in assessing behavioural intentions and equating such behavioural intentions with actual behaviours in information security; secondly, to present an information security policy compliance reinforcement and assessment framework which assists in promoting the conversion of intentions into actual behaviours and in assessing the behavioural change. The approach used was based on the Theory of Planned Behaviour, knowledge, attitude and behaviour theory and Deterrence Theory. Expert review and action research methods were used to validate and refine the framework. The action research was rigorously conducted in four iterations at an SME in South Africa and involved 30 participating employees. The main findings of the study revealed that even though employees may have been well trained and are aware of information security good practice, they may be either unable or unwilling to comply with such practice. The findings of the study also revealed that awareness drives which lead to secure behavioural intents are merely a first step in information security compliance. The study found that not all behavioural intentions converted to actual secure behaviours and only 64% converted. However, deterrence using rewards for good behaviour and punishment for undesirable behaviour was able to increase the conversion by 21%.
- Full Text:
An information security policy compliance reinforcement and assessment framework
- Authors: Gundu, Tapiwa
- Date: 2017
- Subjects: Computer security Information technology--Security measures Information resources management--Security measures
- Language: English
- Type: Thesis , Doctoral , Information Systems
- Identifier: http://hdl.handle.net/10353/11554 , vital:39084
- Description: The majority of SMEs have adopted the use of information communication and technology (ICT) services. However, this has exposed their systems to new internal and external security vulnerabilities. These SMEs seem more concerned with external threat related vulnerabilities rather than those from internal threats, although researchers and industry are suggesting a substantial proportion of security incidents to be originating from insiders. Internal threat is often addressed by, firstly, a security policy in order to direct activities and, secondly, organisational information security training and awareness programmes. These two approaches aim to ensure that employees are proficient in their roles and that they know how to carry out their responsibilities securely. There has been a significant amount of research conducted to ensure that information security programmes communicate the information security policy effectively and reinforce sound security practice. However, an assessment of the genuine effectiveness of such programmes is seldom carried out. The purposes of this research study were, firstly, to highlight the flaws in assessing behavioural intentions and equating such behavioural intentions with actual behaviours in information security; secondly, to present an information security policy compliance reinforcement and assessment framework which assists in promoting the conversion of intentions into actual behaviours and in assessing the behavioural change. The approach used was based on the Theory of Planned Behaviour, knowledge, attitude and behaviour theory and Deterrence Theory. Expert review and action research methods were used to validate and refine the framework. The action research was rigorously conducted in four iterations at an SME in South Africa and involved 30 participating employees. The main findings of the study revealed that even though employees may have been well trained and are aware of information security good practice, they may be either unable or unwilling to comply with such practice. The findings of the study also revealed that awareness drives which lead to secure behavioural intents are merely a first step in information security compliance. The study found that not all behavioural intentions converted to actual secure behaviours and only 64percent converted. However, deterrence using rewards for good behaviour and punishment for undesirable behaviour was able to increase the conversion by 21percent.
- Full Text:
- Authors: Gundu, Tapiwa
- Date: 2017
- Subjects: Computer security Information technology--Security measures Information resources management--Security measures
- Language: English
- Type: Thesis , Doctoral , Information Systems
- Identifier: http://hdl.handle.net/10353/11554 , vital:39084
- Description: The majority of SMEs have adopted the use of information communication and technology (ICT) services. However, this has exposed their systems to new internal and external security vulnerabilities. These SMEs seem more concerned with external threat related vulnerabilities rather than those from internal threats, although researchers and industry are suggesting a substantial proportion of security incidents to be originating from insiders. Internal threat is often addressed by, firstly, a security policy in order to direct activities and, secondly, organisational information security training and awareness programmes. These two approaches aim to ensure that employees are proficient in their roles and that they know how to carry out their responsibilities securely. There has been a significant amount of research conducted to ensure that information security programmes communicate the information security policy effectively and reinforce sound security practice. However, an assessment of the genuine effectiveness of such programmes is seldom carried out. The purposes of this research study were, firstly, to highlight the flaws in assessing behavioural intentions and equating such behavioural intentions with actual behaviours in information security; secondly, to present an information security policy compliance reinforcement and assessment framework which assists in promoting the conversion of intentions into actual behaviours and in assessing the behavioural change. The approach used was based on the Theory of Planned Behaviour, knowledge, attitude and behaviour theory and Deterrence Theory. Expert review and action research methods were used to validate and refine the framework. The action research was rigorously conducted in four iterations at an SME in South Africa and involved 30 participating employees. The main findings of the study revealed that even though employees may have been well trained and are aware of information security good practice, they may be either unable or unwilling to comply with such practice. The findings of the study also revealed that awareness drives which lead to secure behavioural intents are merely a first step in information security compliance. The study found that not all behavioural intentions converted to actual secure behaviours and only 64percent converted. However, deterrence using rewards for good behaviour and punishment for undesirable behaviour was able to increase the conversion by 21percent.
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An investigation into academic dishonesty at the University of Fort Hare
- Authors: Mapetere, Gabriel Talent
- Date: 2017
- Subjects: Cheating (Education) Education, Higher -- Moral and ethical aspects Cheating (Education) -- Prevention
- Language: English
- Type: Thesis , Masters , M. Soc. Sc
- Identifier: http://hdl.handle.net/10353/9588 , vital:34795
- Description: This study investigated the phenomenon of academic dishonesty among university students using a case study of the University of Fort Hare in South Africa. Objectives of this study were to find out the incidence of academic dishonesty, factors associated with academic dishonesty, the frequency of academic dishonesty and mitigation measures placed to curb academic dishonesty at the University of Fort Hare. A mixed methods approach was used to collect data, which was then interpreted using the social learning theory. The study argues that, academic dishonesty at the University of Fort Hare is mainly entrenched in the dynamic transformations brought about by technological advancement, which has given rise to new forms of academic dishonesty and as well facilitated the existent forms of academic dishonesty. The social learning theory offers an explanation of the acquisition of new behaviour and its maintenance thereof hence it has been adopted as the theoretical framework in this study. Findings of the current study indicated that the institution, like many other institutions globally is affected by academic dishonesty. Apart from that, academic dishonesty in its various forms is significantly high and it continues to soar. Moreover, the study revealed that the institution has adopted a diverse array of measures to curb academic dishonesty. Nevertheless, students continue to engage in academic dishonesty regardless of efforts made by institutions to reduce this epidemic. There is therefore a need to revisit the policies and measures implemented by institutions to curb academic dishonesty in a bid to come up with effective solutions to this problem.
- Full Text:
- Authors: Mapetere, Gabriel Talent
- Date: 2017
- Subjects: Cheating (Education) Education, Higher -- Moral and ethical aspects Cheating (Education) -- Prevention
- Language: English
- Type: Thesis , Masters , M. Soc. Sc
- Identifier: http://hdl.handle.net/10353/9588 , vital:34795
- Description: This study investigated the phenomenon of academic dishonesty among university students using a case study of the University of Fort Hare in South Africa. Objectives of this study were to find out the incidence of academic dishonesty, factors associated with academic dishonesty, the frequency of academic dishonesty and mitigation measures placed to curb academic dishonesty at the University of Fort Hare. A mixed methods approach was used to collect data, which was then interpreted using the social learning theory. The study argues that, academic dishonesty at the University of Fort Hare is mainly entrenched in the dynamic transformations brought about by technological advancement, which has given rise to new forms of academic dishonesty and as well facilitated the existent forms of academic dishonesty. The social learning theory offers an explanation of the acquisition of new behaviour and its maintenance thereof hence it has been adopted as the theoretical framework in this study. Findings of the current study indicated that the institution, like many other institutions globally is affected by academic dishonesty. Apart from that, academic dishonesty in its various forms is significantly high and it continues to soar. Moreover, the study revealed that the institution has adopted a diverse array of measures to curb academic dishonesty. Nevertheless, students continue to engage in academic dishonesty regardless of efforts made by institutions to reduce this epidemic. There is therefore a need to revisit the policies and measures implemented by institutions to curb academic dishonesty in a bid to come up with effective solutions to this problem.
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An Investigation into the community's experience of the land claim process: the case study of Cwengcwe village in King Williams Town, Eastern Cape
- Authors: Mgweba, Unati Natashe
- Date: 2017
- Subjects: Land reform -- South Africa , Restitution , Black people -- South Africa
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/26808 , vital:66011
- Description: This study examines Cwengcwe community’s experiences of the land claim process. This community is located in King William’s Town, Eastern Cape. The land issue remains unfinished business in the South African context with many land claimants still waiting to receive their land back or to be compensated for their land with money. Cwengcwe community members are not an exception in the channel of claiming back their land and having to wait for a lengthy period of time to be compensated. The main objective of the study was therefore to find out about the experiences of the Cwengcwe community with regards to the land claims process. The study adopted a qualitative research approach and the data was collected by conducting semi-structured interviews and observations. Purposive sampling was used to collect data from community members at Cwengcwe village. The findings of the study reveal that the community members in this village are very disappointed at the slow progress of processing the land claims, as their claims which were lodged in 2015 had not been settled as yet in November 2017. Furthermore, the minimal and/or lack of communication from the Department of Rural Development and Land Reform was a negative indicator in the prioritisation ladder. This study recommends further exploration of the land claim process in order to review its model and come up with alternative processes that might ease the land claim process. , Thesis (MSoc) -- Faculty of Social Sciences and Humanities, 2017
- Full Text:
- Authors: Mgweba, Unati Natashe
- Date: 2017
- Subjects: Land reform -- South Africa , Restitution , Black people -- South Africa
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/26808 , vital:66011
- Description: This study examines Cwengcwe community’s experiences of the land claim process. This community is located in King William’s Town, Eastern Cape. The land issue remains unfinished business in the South African context with many land claimants still waiting to receive their land back or to be compensated for their land with money. Cwengcwe community members are not an exception in the channel of claiming back their land and having to wait for a lengthy period of time to be compensated. The main objective of the study was therefore to find out about the experiences of the Cwengcwe community with regards to the land claims process. The study adopted a qualitative research approach and the data was collected by conducting semi-structured interviews and observations. Purposive sampling was used to collect data from community members at Cwengcwe village. The findings of the study reveal that the community members in this village are very disappointed at the slow progress of processing the land claims, as their claims which were lodged in 2015 had not been settled as yet in November 2017. Furthermore, the minimal and/or lack of communication from the Department of Rural Development and Land Reform was a negative indicator in the prioritisation ladder. This study recommends further exploration of the land claim process in order to review its model and come up with alternative processes that might ease the land claim process. , Thesis (MSoc) -- Faculty of Social Sciences and Humanities, 2017
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An investigation of experiences encountered by female adults living with HIV/AIDS and taking ARV treatment: the case of Ntselanzi location, Raymond Mhlaba municipality, Alice Eastern Cape
- Authors: Matumelo, Nobuhle
- Date: 2017
- Subjects: AIDS (Disease) -- South Africa -- Eastern Cape -- Prevention , HIV-positive persons -- South Africa -- Eastern Cape , HIV infections -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSoc
- Identifier: http://hdl.handle.net/10353/15012 , vital:40132
- Description: The study investigated the experiences encountered by female adults from Ntselamanzi living with Human Immunodeficiency Virus/ Acquired Immune Deficiency Syndrome (HIV/AIDS) and using Anti-retroviral (ARV) treatment. The study used a qualitative approach as well as a case study design that was descriptive explorative and explanative. The sample size or the unit of analysis consisted of 29 participants who included 12 female adults living with HIV/AIDS and having used ARVs for a minimum of three months; six nurses directly serving the people living with HIV/AIDS (PLWHA); six family members catering for the infected members within their family setting; and five key informants that included a social worker, a community leader, a health promoter, a community developer, and an HIV co-ordinator situated within the community of Ntselamanzi. A non-probability sampling method was adopted, specifically incorporating both snowball and purposive sampling techniques to select the sample. Significantly, the study was led by the following objectives: (1) To explore the experiences of female adults living with HIV/AIDS in Ntselamanzi, (2) To establish the coping mechanisms adopted by the PLWHA (3) To determine the effects of coping challenges and the adherence to ARVs. The data was collected by means of face-toface in-depth interviews in tandem with focus group discussion (FGD); and it was analysed qualitatively using content thematic analysis. The findings of the study revealed that all female adults living with HIV/AIDS and using ARVs encountered innumerable challenges that impaired their coping. The findings also indicated that malfunctioning and interruption of PLWHA coping was influenced by social factors such as unemployment, high level of poverty, low educational levels, lack of information sharing programmes and dependency on social grants. Lastly, the support to PLWHA especially by family members was highly indicated in the study’s findings. The following recommendations emanated from the study: strengthening research on coping challenges of female adults using ARVs in rural areas; empowering and training more caregivers for PLWHA by the Health Department; more involvement of social workers in helping the PLWHA; more involvement of government in the HIV/AIDS campaign in rural settings; and government facilitation and support to the PLWHA to form and belong to support groups.
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- Authors: Matumelo, Nobuhle
- Date: 2017
- Subjects: AIDS (Disease) -- South Africa -- Eastern Cape -- Prevention , HIV-positive persons -- South Africa -- Eastern Cape , HIV infections -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSoc
- Identifier: http://hdl.handle.net/10353/15012 , vital:40132
- Description: The study investigated the experiences encountered by female adults from Ntselamanzi living with Human Immunodeficiency Virus/ Acquired Immune Deficiency Syndrome (HIV/AIDS) and using Anti-retroviral (ARV) treatment. The study used a qualitative approach as well as a case study design that was descriptive explorative and explanative. The sample size or the unit of analysis consisted of 29 participants who included 12 female adults living with HIV/AIDS and having used ARVs for a minimum of three months; six nurses directly serving the people living with HIV/AIDS (PLWHA); six family members catering for the infected members within their family setting; and five key informants that included a social worker, a community leader, a health promoter, a community developer, and an HIV co-ordinator situated within the community of Ntselamanzi. A non-probability sampling method was adopted, specifically incorporating both snowball and purposive sampling techniques to select the sample. Significantly, the study was led by the following objectives: (1) To explore the experiences of female adults living with HIV/AIDS in Ntselamanzi, (2) To establish the coping mechanisms adopted by the PLWHA (3) To determine the effects of coping challenges and the adherence to ARVs. The data was collected by means of face-toface in-depth interviews in tandem with focus group discussion (FGD); and it was analysed qualitatively using content thematic analysis. The findings of the study revealed that all female adults living with HIV/AIDS and using ARVs encountered innumerable challenges that impaired their coping. The findings also indicated that malfunctioning and interruption of PLWHA coping was influenced by social factors such as unemployment, high level of poverty, low educational levels, lack of information sharing programmes and dependency on social grants. Lastly, the support to PLWHA especially by family members was highly indicated in the study’s findings. The following recommendations emanated from the study: strengthening research on coping challenges of female adults using ARVs in rural areas; empowering and training more caregivers for PLWHA by the Health Department; more involvement of social workers in helping the PLWHA; more involvement of government in the HIV/AIDS campaign in rural settings; and government facilitation and support to the PLWHA to form and belong to support groups.
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An investigation of the contribution of street vending on livelihoods : case of street vendors in Nkonkobe Municipality
- Authors: Dube, Thulani
- Date: 2017
- Subjects: Street vendors -- South Africa -- Eastern Cape Street vendors -- South Africa -- Eastern Cape -- Economic conditions Vending stands -- Economic aspects -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSoc
- Identifier: http://hdl.handle.net/10353/6068 , vital:29488
- Description: The informal sector thrives in a context of high unemployment, underemployment, poverty, gender inequality and precarious work. It plays a significant role in such circumstances, especially in income generation and in contributing to the livelihood of those engaged in it. The study investigated the contribution of street vending to the livelihood of street vendors in Nkonkobe Municipality. The aim of the study was to investigate whether street vending has the potential to sustain the lives of those engaged in it. In order to reach its objectives, the study employed a qualitative research approach whereby a non-probability sampling design in the form of a purposive sampling method was adopted and considered appropriate for this study. In terms of data collection, the study made use of structured questionnaires as a data collection method whereby questionnaires were administered and distributed to 40 street venders. The Upper Bound Poverty Line (UBPL) was used as a measurement to assess whether the participants’ incomes were above the poverty datum line in order to determine the sector’s contribution to the traders’ livelihood. From this study, it was found that street vending has both positive and negative impacts with regard to the success and profit-earning potential of these traders. Thus, the study concluded that street vending is a vital community development tool and a major source of livelihood as it plays a very important socio-economic role in Nkonkobe and in South Africa in general. The sector provides a livelihood for a huge population mainly comprised of the poor and other impoverished groups of society.
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- Authors: Dube, Thulani
- Date: 2017
- Subjects: Street vendors -- South Africa -- Eastern Cape Street vendors -- South Africa -- Eastern Cape -- Economic conditions Vending stands -- Economic aspects -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSoc
- Identifier: http://hdl.handle.net/10353/6068 , vital:29488
- Description: The informal sector thrives in a context of high unemployment, underemployment, poverty, gender inequality and precarious work. It plays a significant role in such circumstances, especially in income generation and in contributing to the livelihood of those engaged in it. The study investigated the contribution of street vending to the livelihood of street vendors in Nkonkobe Municipality. The aim of the study was to investigate whether street vending has the potential to sustain the lives of those engaged in it. In order to reach its objectives, the study employed a qualitative research approach whereby a non-probability sampling design in the form of a purposive sampling method was adopted and considered appropriate for this study. In terms of data collection, the study made use of structured questionnaires as a data collection method whereby questionnaires were administered and distributed to 40 street venders. The Upper Bound Poverty Line (UBPL) was used as a measurement to assess whether the participants’ incomes were above the poverty datum line in order to determine the sector’s contribution to the traders’ livelihood. From this study, it was found that street vending has both positive and negative impacts with regard to the success and profit-earning potential of these traders. Thus, the study concluded that street vending is a vital community development tool and a major source of livelihood as it plays a very important socio-economic role in Nkonkobe and in South Africa in general. The sector provides a livelihood for a huge population mainly comprised of the poor and other impoverished groups of society.
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An investigation on the motivational feattures influence on technical efficiency and profitability of smallholder irrigated crop farmers in Kwa-Nobuhle, Eastern Cape province of South Africa
- Authors: Funde, Sibongile
- Date: 2017
- Subjects: Crops -- South Africa -- Eastern Cape -- Marketing Irrigation farming -- South Africa -- Eastern Cape Agriculture -- Economic aspects -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/11917 , vital:39118
- Description: Substantial impact and the importance of agriculture in the economies of the developing countries is increasingly acknowledged. Agriculture generally has shown to remain crucial to economic development. It has been noted that agricultural growth plays a pivotal role in poverty reduction and employment creation. Due to the potential of the smallholder sector to alleviate poverty and minimize unemployment rate leading to the country’s economic growth, enormous development programs have in the past and continues to be implemented by South African government. Programs such as land-based development strategies including agriculture, extension services, improved technologies including irrigation establishment, revitalization and rehabilitation, water rights legislation and financial support. However, the current state of smallholder low-agricultural productivity growth trends raises concerns on what other fundamental development strategies to implement in order to enhance agricultural productivity growth. Hence, this study seeks to address the impact of social farming on agricultural productivity by investigating smallholder farmer’s motivational features. Motivational features regarded are as important determinants of success, modify behaviour, serve to mobilize and direct energy into actions. Given the importance of motivational features and their formation in social interaction, the objective of the study was to investigate motivational features of smallholder farmers with regard to expanding irrigation land, determine technical efficiency of smallholder farmers as well as their profitability generation capability. A multistage sampling method was used that involved using purposive sampling, simple random sampling was adopted where three smallholder agriculture projects using irrigation methods to farm were selected and by use of snowball sampling the study ended up collecting data from a total of 124 respondents. The sample was chosen on the basis that smallholder farmers hold attributes that were crucial for execution of this research. The study proceeded by conducting face-to face interviews of smallholder farmers, focus groups and key informant using interview schedules and a questionnaire consisting both open and close-ended questions. Data were analysed using different methods. Firstly, data were analysed descriptively to get a deeper understanding of smallholder farmer’s socio-economic characteristics as well as their motivational features. Secondly, data were analysed statistically to determine Technical Efficiency (TE) of smallholder farmers using both Data Envelopment Analysis (DEA) and Stochastic Frontier Analysis (SFA). Lastly, Gross Margin Analysis was used to measure farmer’s profitability. Results show that smallholder farmers have a range of motivational features that incorporates both productive and non-productive goals. The study made use of twenty-five goals obtained from literature studies and categorised them into six motivational feature types namely; outcome based, process focused/extrinsic, performance, achievement, intrinsic/self-efficacy, and household livelihood strategies. Smallholder female and male outcome based motivations were found to be statistically significant at 5percent, while process focused/extrinsic, performance, achievement, intrinsic/self-efficacy and household livelihood strategies motivations between them were found not to be statistically significant, which means that smallholder female and male motivations are not different and that both farmers consider such motivations at the same level. Technical Efficiency (TE) was measured using DEA method and was found to be 0.70, which is different from the TE obtained using SFA that was 0.47. Three dominant crops were produced, namely potatoes, cabbage and spinach. Cabbage crop was the most produced, had the highest gross margin results with a commercialization ratio of 0.33percent as compared to potatoes and spinach. Which indicates that cabbage is a high value crop to smallholder farmers of Kwa-Nobuhle area. In view of the research findings, it was concluded that motivational features of smallholder farmers were positively related to technical efficiency which implies that the more motivated farmers become, the higher their technical efficiency, hence their potential to expand land under irrigation. Cabbage production was high and more profitable than potatoes and spinach production. Recommendations include that government, NGO’s, private sector and institution need to promote the use of improved technologies, agricultural training and take smallholder farmer’s motivational features into account in development programmes. These actions are necessary to increase irrigated farming, overcome low-performance levels and enhance profitability of smallholder farmers, and ultimately achieve poverty alleviation and improve employment opportunities in the rural areas.
- Full Text:
- Authors: Funde, Sibongile
- Date: 2017
- Subjects: Crops -- South Africa -- Eastern Cape -- Marketing Irrigation farming -- South Africa -- Eastern Cape Agriculture -- Economic aspects -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/11917 , vital:39118
- Description: Substantial impact and the importance of agriculture in the economies of the developing countries is increasingly acknowledged. Agriculture generally has shown to remain crucial to economic development. It has been noted that agricultural growth plays a pivotal role in poverty reduction and employment creation. Due to the potential of the smallholder sector to alleviate poverty and minimize unemployment rate leading to the country’s economic growth, enormous development programs have in the past and continues to be implemented by South African government. Programs such as land-based development strategies including agriculture, extension services, improved technologies including irrigation establishment, revitalization and rehabilitation, water rights legislation and financial support. However, the current state of smallholder low-agricultural productivity growth trends raises concerns on what other fundamental development strategies to implement in order to enhance agricultural productivity growth. Hence, this study seeks to address the impact of social farming on agricultural productivity by investigating smallholder farmer’s motivational features. Motivational features regarded are as important determinants of success, modify behaviour, serve to mobilize and direct energy into actions. Given the importance of motivational features and their formation in social interaction, the objective of the study was to investigate motivational features of smallholder farmers with regard to expanding irrigation land, determine technical efficiency of smallholder farmers as well as their profitability generation capability. A multistage sampling method was used that involved using purposive sampling, simple random sampling was adopted where three smallholder agriculture projects using irrigation methods to farm were selected and by use of snowball sampling the study ended up collecting data from a total of 124 respondents. The sample was chosen on the basis that smallholder farmers hold attributes that were crucial for execution of this research. The study proceeded by conducting face-to face interviews of smallholder farmers, focus groups and key informant using interview schedules and a questionnaire consisting both open and close-ended questions. Data were analysed using different methods. Firstly, data were analysed descriptively to get a deeper understanding of smallholder farmer’s socio-economic characteristics as well as their motivational features. Secondly, data were analysed statistically to determine Technical Efficiency (TE) of smallholder farmers using both Data Envelopment Analysis (DEA) and Stochastic Frontier Analysis (SFA). Lastly, Gross Margin Analysis was used to measure farmer’s profitability. Results show that smallholder farmers have a range of motivational features that incorporates both productive and non-productive goals. The study made use of twenty-five goals obtained from literature studies and categorised them into six motivational feature types namely; outcome based, process focused/extrinsic, performance, achievement, intrinsic/self-efficacy, and household livelihood strategies. Smallholder female and male outcome based motivations were found to be statistically significant at 5percent, while process focused/extrinsic, performance, achievement, intrinsic/self-efficacy and household livelihood strategies motivations between them were found not to be statistically significant, which means that smallholder female and male motivations are not different and that both farmers consider such motivations at the same level. Technical Efficiency (TE) was measured using DEA method and was found to be 0.70, which is different from the TE obtained using SFA that was 0.47. Three dominant crops were produced, namely potatoes, cabbage and spinach. Cabbage crop was the most produced, had the highest gross margin results with a commercialization ratio of 0.33percent as compared to potatoes and spinach. Which indicates that cabbage is a high value crop to smallholder farmers of Kwa-Nobuhle area. In view of the research findings, it was concluded that motivational features of smallholder farmers were positively related to technical efficiency which implies that the more motivated farmers become, the higher their technical efficiency, hence their potential to expand land under irrigation. Cabbage production was high and more profitable than potatoes and spinach production. Recommendations include that government, NGO’s, private sector and institution need to promote the use of improved technologies, agricultural training and take smallholder farmer’s motivational features into account in development programmes. These actions are necessary to increase irrigated farming, overcome low-performance levels and enhance profitability of smallholder farmers, and ultimately achieve poverty alleviation and improve employment opportunities in the rural areas.
- Full Text:
An optimum wind power output model for concentrator augmented wind turbines (CAWTs)
- Authors: Shonhiwa, Chipo
- Date: 2017
- Subjects: Wind power Wind turbines
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10353/8692 , vital:33427
- Description: The aim of this study was to develop an optimum wind power output model for concentrator augmented wind turbines (CAWTs), which can operate in low wind speed areas. This study was done using computational fluid dynamics modelling (CFD), theoretical and experimental work. Currently the wind resource is contributing 2.5percent of the global electricity supply and is anticipated to increase to by 2050. Despite this, the contribution to wind energy consumption share by South Africa is still low having just exceeded in 2015. Like many other countries, South Africa agrees that climate change is one of the biggest challenges currently being faced by mankind and acknowledges that tackling it requires collaborative effort from all. The Integrated Resource Plan (IRP 2010-2030) passed in 2010 targets 26.3percent renewable energy contribution to national energy consumption by the year 2030 with 10.3percent coming from wind energy hence contributing to abatement of climate change. 1518percent 1 000 MW Small wind turbines are known for their vital contribution to providing electricity in remote areas. Their power output is mainly influenced by the turbine rotor diameter and wind speed which depend on tower height. A cost benefit analysis conducted basing on increasing wind speed by increasing the turbine tower height revealed that for small wind turbines, the optimal height is . Concentrator Augmented Wind Turbines (CAWTs) are one of several concepts that have been suggested to increase the power output per unit rotor cross-sectional area by increasing the wind speed upstream of the turbine. Although some research effort has been put in this area, the major drawback is that the effect of concentrator geometry and environmental conditions on the aerodynamics and hence ultimate performance of CAWTs is still not clear. Also, the CAWTs systems that have been made so far, do not track the wind resulting in CAWT systems receiving wind from one direction thus limiting their performance. 25 m Wind has a very low energy density in nature implying that a bigger rotor area is required to harness kinetic energy for generation of meaningful electrical energy. This has resulted in higher costs of producing energy from wind than conventional sources of energy such as fossil fuels and hydro. To lower the cost of wind energy, researchers have come up with several initiatives to boost wind turbine power output. A brief review of various concepts that have been used in this study included: history of wind turbines, South Africa’s political will to abatement of climate change, wind resource assessment, power output augmentation, estimation of air density, historical development of CAWTs, fundamental parameters influencing the performance of CAWTs, mathematical modelling of wind turbines and dynamic similarity. Some facts were borrowed from these concepts to come up with the current study and explain the observations. With a view to assess any concept, it is necessary to study the fundamental basis from which it originates. Thus, it was vital to develop a fundamental basis of CAWT performance from which solutions to currently prevailing questions on the relationships among the governing parameters were obtained. The momentum theory of bare wind turbines, which is also found in literature was described. A similar approach was then used for developing the theory of CAWT systems. The theories of concentrator losses, density variation with weather conditions and turbulence modelling were also described. It was of utmost significance to evaluate the wind power potential of the Western part of the Eastern Cape Province to assess whether there would be a need for the concentrator augmented wind turbines (CAWTs) in the province and all other regions with similar or lower wind speeds in the country. In this study, five-year-long, hourly average wind speed series between January 2009 and December 2013 for six weather stations in the western part of the Eastern Cape (Bisho, Fort Beaufort, Graaff–Reinet, Grahamstown, Port Elizabeth and Queenstown) were statistically analysed using the Weibull distribution function. The dimensionless Weibull shape parameter varied from 1.7 to 2.2. All the values were low indicating that there is widely dispersed data. This means that the data tend to be distributed uniformly over a relatively wide range wind speed. Therefore, this has a positive implication on wind power generation in all the studied areas because if a suitable turbine is installed, it would get enough wind speed to run for the greater part of the year. The scale parameter varied between 3.5 and 6.3. The values for all the sites except Port Elizabeth were relatively low implying that the days of high wind speeds are few. Therefore, wind turbines that require high wind speeds for operation are not suitable here.
- Full Text:
- Authors: Shonhiwa, Chipo
- Date: 2017
- Subjects: Wind power Wind turbines
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10353/8692 , vital:33427
- Description: The aim of this study was to develop an optimum wind power output model for concentrator augmented wind turbines (CAWTs), which can operate in low wind speed areas. This study was done using computational fluid dynamics modelling (CFD), theoretical and experimental work. Currently the wind resource is contributing 2.5percent of the global electricity supply and is anticipated to increase to by 2050. Despite this, the contribution to wind energy consumption share by South Africa is still low having just exceeded in 2015. Like many other countries, South Africa agrees that climate change is one of the biggest challenges currently being faced by mankind and acknowledges that tackling it requires collaborative effort from all. The Integrated Resource Plan (IRP 2010-2030) passed in 2010 targets 26.3percent renewable energy contribution to national energy consumption by the year 2030 with 10.3percent coming from wind energy hence contributing to abatement of climate change. 1518percent 1 000 MW Small wind turbines are known for their vital contribution to providing electricity in remote areas. Their power output is mainly influenced by the turbine rotor diameter and wind speed which depend on tower height. A cost benefit analysis conducted basing on increasing wind speed by increasing the turbine tower height revealed that for small wind turbines, the optimal height is . Concentrator Augmented Wind Turbines (CAWTs) are one of several concepts that have been suggested to increase the power output per unit rotor cross-sectional area by increasing the wind speed upstream of the turbine. Although some research effort has been put in this area, the major drawback is that the effect of concentrator geometry and environmental conditions on the aerodynamics and hence ultimate performance of CAWTs is still not clear. Also, the CAWTs systems that have been made so far, do not track the wind resulting in CAWT systems receiving wind from one direction thus limiting their performance. 25 m Wind has a very low energy density in nature implying that a bigger rotor area is required to harness kinetic energy for generation of meaningful electrical energy. This has resulted in higher costs of producing energy from wind than conventional sources of energy such as fossil fuels and hydro. To lower the cost of wind energy, researchers have come up with several initiatives to boost wind turbine power output. A brief review of various concepts that have been used in this study included: history of wind turbines, South Africa’s political will to abatement of climate change, wind resource assessment, power output augmentation, estimation of air density, historical development of CAWTs, fundamental parameters influencing the performance of CAWTs, mathematical modelling of wind turbines and dynamic similarity. Some facts were borrowed from these concepts to come up with the current study and explain the observations. With a view to assess any concept, it is necessary to study the fundamental basis from which it originates. Thus, it was vital to develop a fundamental basis of CAWT performance from which solutions to currently prevailing questions on the relationships among the governing parameters were obtained. The momentum theory of bare wind turbines, which is also found in literature was described. A similar approach was then used for developing the theory of CAWT systems. The theories of concentrator losses, density variation with weather conditions and turbulence modelling were also described. It was of utmost significance to evaluate the wind power potential of the Western part of the Eastern Cape Province to assess whether there would be a need for the concentrator augmented wind turbines (CAWTs) in the province and all other regions with similar or lower wind speeds in the country. In this study, five-year-long, hourly average wind speed series between January 2009 and December 2013 for six weather stations in the western part of the Eastern Cape (Bisho, Fort Beaufort, Graaff–Reinet, Grahamstown, Port Elizabeth and Queenstown) were statistically analysed using the Weibull distribution function. The dimensionless Weibull shape parameter varied from 1.7 to 2.2. All the values were low indicating that there is widely dispersed data. This means that the data tend to be distributed uniformly over a relatively wide range wind speed. Therefore, this has a positive implication on wind power generation in all the studied areas because if a suitable turbine is installed, it would get enough wind speed to run for the greater part of the year. The scale parameter varied between 3.5 and 6.3. The values for all the sites except Port Elizabeth were relatively low implying that the days of high wind speeds are few. Therefore, wind turbines that require high wind speeds for operation are not suitable here.
- Full Text:
Analyses of the simple harmonic oscillator and its variations
- Authors: Bambiso, Noludwe Patience
- Date: 2017
- Subjects: Harmonic analysis Vibration -- Mathematical models Oscillations
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/8132 , vital:31530
- Description: Vibratory motion is fundamental to all natural phenomena. It occurs everywhere in nature, from fundamental particles to the complexity of the life of the smallest cell, to the properties of the cosmos. Its frequency ranges from those of gammarays down the spectrum of X-rays, microwave, optical and radio waves. These are common concepts in everyday life, but their origin and nature are seldom comprehended. In this thesis we look at the mathematical fundamentals and how to apply them. We start with fundamental concepts and extend them to more complicated systems. We consider methods of analysis and applications. In Chapter 2 we consider simple harmonic oscillators and their mathematical analyses and in Chapter 3 we look at applications. Chapter 4 discusses the general form of simple harmonic motion, i.e. damped and forced harmonic motion. We consider simple coupled systems and their analysis. In Chapter 5 we introduce the method of complex functions and its application to complicated compound systems. Chapter 6 introduces a novel application, that of how SHM can be used as a model for advanced effects, or acausality. Chapter 7 presents a summary and conclusion.
- Full Text:
- Authors: Bambiso, Noludwe Patience
- Date: 2017
- Subjects: Harmonic analysis Vibration -- Mathematical models Oscillations
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/8132 , vital:31530
- Description: Vibratory motion is fundamental to all natural phenomena. It occurs everywhere in nature, from fundamental particles to the complexity of the life of the smallest cell, to the properties of the cosmos. Its frequency ranges from those of gammarays down the spectrum of X-rays, microwave, optical and radio waves. These are common concepts in everyday life, but their origin and nature are seldom comprehended. In this thesis we look at the mathematical fundamentals and how to apply them. We start with fundamental concepts and extend them to more complicated systems. We consider methods of analysis and applications. In Chapter 2 we consider simple harmonic oscillators and their mathematical analyses and in Chapter 3 we look at applications. Chapter 4 discusses the general form of simple harmonic motion, i.e. damped and forced harmonic motion. We consider simple coupled systems and their analysis. In Chapter 5 we introduce the method of complex functions and its application to complicated compound systems. Chapter 6 introduces a novel application, that of how SHM can be used as a model for advanced effects, or acausality. Chapter 7 presents a summary and conclusion.
- Full Text:
Analysis of the impact of Smallholder Irrigation Schemes on the choice of rural livelihood strategy and household food security in Eastern Cape
- Authors: Christian, Mzuyanda
- Date: 2017
- Subjects: Irrigation -- South Africa -- Eastern Cape Farms, Small -- South Africa -- Eastern Cape Food security -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10353/4813 , vital:28526
- Description: Since the end of Apartheid, the South African government has invested substantially in smallholder irrigation schemes, particularly in the former homeland areas. One of the primary goals for establishment of these schemes was to solve food security problems. Sadly, these efforts have not produced the desired results, and poverty has deepened. The question that can be asked is: What is it that makes South African smallholder agricultural sector seemingly impervious to the interventions that government has made in targeting smallholder farmers since the dawn of democracy in 1994? In order to address such a question, a set of structured questionnaires was used to interview 100 smallholder irrigators and 100 non-irrigators using multistage stratified random sampling technique in the Amatole, Chris Hani and OR Tambo District municipalities. Demographic and socio-economic characteristics of smallholder irrigators and non-irrigators around Qamata, Pendu, Tyhefu, Merelles and Ntshongweni irrigation schemes were measured using descriptive statistics. Profitability between the two groups was measured using gross margin analysis (GM). The Probit model was used to determine the factors that affect smallholder farmer‘s choice of participation in irrigation schemes. The impact of irrigation participation on yields, crop sales and food security was estimated using the propensity score matching (PSM) technique. The impact of irrigation participation of smallholder farmer‘s choice of livelihood strategy was estimated using the multinomial regression model. The results showed that male respondents were dominant with a representation of 63 percent and average mean household size of 4 persons with the household head having spent at least 7 years at school. Farming was found to be the major (64 percent) occupation in the study area with an average income of R2 944.52 per cropping season. Both irrigating and non-irrigating smallholder farmers in the study area used improved, fertilizer and tractor for production. Overall, smallholder farmers spent 30.9 percent of their household income on food with irrigators‘food spending lower than those of non-irrigators. The gross margin analysis suggests that the irrigated farmers were in a better position to afford enough food in order to satisfy their household requirement. Findings from the Probit regression and Propensity Score Matching are consistent across the methods, indicating that irrigation participation has a positive influence on crop yields, income and consumption expenditure. Probit regression further showed that age, quality of land, access to credit, access to market, distance to the scheme and membership of another society/association have a positive impact on access and use of irrigation and are significant at p < 0.05 percent level. The Propensity Score Matching using Nearest Neighbour and Kernel Matching Methods of the outcome variables, total farm income and food consumption patterns, shows a positive and statistically significant result at p < 0.05 percent level. The nearest neighbour matching method shows that irrigators received higher farm income R2044.01 than non-irrigating farmers R622.12. The Average Treatment Effect on total food expenditure was negative both in the case of NNM and KM algorithms, indicating that participation in irrigation can decrease the expenditure levels on food from R933.30 to R926.70. This could be due to the fact that irrigating farmers produced enough for home consumption than non-irrigators. The multinomial regression model shows that irrigation participation influences smallholder farmers choice of livelihood strategy and these variables were statistically significant at p < 0.05 percent level. These results provide insights to address the question as to the appropriate development path for transition from homestead to irrigation. Continued support to smallholder farmers in the forms of funding, extension services and improved technologies is certainly needed to enhance food security.
- Full Text:
- Authors: Christian, Mzuyanda
- Date: 2017
- Subjects: Irrigation -- South Africa -- Eastern Cape Farms, Small -- South Africa -- Eastern Cape Food security -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10353/4813 , vital:28526
- Description: Since the end of Apartheid, the South African government has invested substantially in smallholder irrigation schemes, particularly in the former homeland areas. One of the primary goals for establishment of these schemes was to solve food security problems. Sadly, these efforts have not produced the desired results, and poverty has deepened. The question that can be asked is: What is it that makes South African smallholder agricultural sector seemingly impervious to the interventions that government has made in targeting smallholder farmers since the dawn of democracy in 1994? In order to address such a question, a set of structured questionnaires was used to interview 100 smallholder irrigators and 100 non-irrigators using multistage stratified random sampling technique in the Amatole, Chris Hani and OR Tambo District municipalities. Demographic and socio-economic characteristics of smallholder irrigators and non-irrigators around Qamata, Pendu, Tyhefu, Merelles and Ntshongweni irrigation schemes were measured using descriptive statistics. Profitability between the two groups was measured using gross margin analysis (GM). The Probit model was used to determine the factors that affect smallholder farmer‘s choice of participation in irrigation schemes. The impact of irrigation participation on yields, crop sales and food security was estimated using the propensity score matching (PSM) technique. The impact of irrigation participation of smallholder farmer‘s choice of livelihood strategy was estimated using the multinomial regression model. The results showed that male respondents were dominant with a representation of 63 percent and average mean household size of 4 persons with the household head having spent at least 7 years at school. Farming was found to be the major (64 percent) occupation in the study area with an average income of R2 944.52 per cropping season. Both irrigating and non-irrigating smallholder farmers in the study area used improved, fertilizer and tractor for production. Overall, smallholder farmers spent 30.9 percent of their household income on food with irrigators‘food spending lower than those of non-irrigators. The gross margin analysis suggests that the irrigated farmers were in a better position to afford enough food in order to satisfy their household requirement. Findings from the Probit regression and Propensity Score Matching are consistent across the methods, indicating that irrigation participation has a positive influence on crop yields, income and consumption expenditure. Probit regression further showed that age, quality of land, access to credit, access to market, distance to the scheme and membership of another society/association have a positive impact on access and use of irrigation and are significant at p < 0.05 percent level. The Propensity Score Matching using Nearest Neighbour and Kernel Matching Methods of the outcome variables, total farm income and food consumption patterns, shows a positive and statistically significant result at p < 0.05 percent level. The nearest neighbour matching method shows that irrigators received higher farm income R2044.01 than non-irrigating farmers R622.12. The Average Treatment Effect on total food expenditure was negative both in the case of NNM and KM algorithms, indicating that participation in irrigation can decrease the expenditure levels on food from R933.30 to R926.70. This could be due to the fact that irrigating farmers produced enough for home consumption than non-irrigators. The multinomial regression model shows that irrigation participation influences smallholder farmers choice of livelihood strategy and these variables were statistically significant at p < 0.05 percent level. These results provide insights to address the question as to the appropriate development path for transition from homestead to irrigation. Continued support to smallholder farmers in the forms of funding, extension services and improved technologies is certainly needed to enhance food security.
- Full Text:
Antidiabetic activity and mechanism of action of extracts of Brachylaena elliptica (Thurb.) DC. and Brachylaena ilicifolia (Lam) Phill & Schweick
- Authors: Sagbo, Idowu Jonas
- Date: 2017
- Subjects: Herbs -- Therapeutic use Pharmacognosy Diabetes
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10353/8154 , vital:31624
- Description: International Diabetes Federation (IDF) estimated that there are 415 million people globally that are suffering from diabetes and this figure is likely to double by 2040. In South Africa, the number of people suffering from diabetes is believed to be rising steadily and the current antidiabetic therapies include the use of expensive pharmaceutical drugs which after display numerous adverse side effects. Herbal medicine is an alternative treatment strategy used by many rural populations for the management of diabetes, which is cost-effective and with minimal reported side effects. The aim of this study was to validate the antidiabetic activity and to elucidate the possible mechanisms of action of the aqueous leaf extracts prepared from two plants, Brachylaena elliptica and Brachylaena ilicifolia, used by traditional healers in the Eastern Cape, South Africa for the treatment of diabetes. The current pharmaceutical drugs target site, including carbohydrate digestion, regulation of blood glucose levels through insulin levels or activating glucose uptake in liver and muscle. The plants extracts were therefore evaluated for their potential to mimic or replace these drugs. The inhibibitory effects on alpha amylase, alpha glucosidase, DPP-IV activity and pancreatic lipase were evaluated at various concentrations of the plant extracts. To evaluate the suitability of these extracts for cell based in vitro assay as well as to determine the safety of the plant extracts for human consumption, cytotoxicity towards HepG2 cells was assessed using the MTT assay. The effect of both plant extracts on glucose uptake in HepG2 and L6 cells, lipid accumulation in 3T3-L1 preadipocytes and NO production in RAW macrophages were investigated. Both plant extracts were also tested for INS-1 proliferation using the imageXpress®Micro XLS analysis. The MTT reduction assay was also used to investigate the effect of extracts on INS-1 glucose metabolism and as a reflection of insulin secretion. The inhibitory activity of the crude extracts of both plants on protein glycation, collagenase activity and CYP3A4 interaction were also evaluated The crude extracts of both plants displayed no significant inhibition on alpha amylase, alpha glucosidase, dipeptidyl peptidase-IV, lipase, protein glycation and collagenase compared to relevant standard inhibitors, acarbose, EGCG, Diprotin A, Orlistart and aminoguanidine. The mild inhibition of B. elliptica on alpha glucosidase activity at the highest concentration of the extract tested, was considered as not physiologically relevant. The aqueous extracts of bothplants showed < 40 percent cell death in HepG2 cells even at high concentration and hence support the use of the plants by traditional healers. The extracts of B. ellipticaat 25 µg/ml, displayed a proliferative effect on INS-1 cells when compared to the negative control and B. ilicifolia at 25 μg/ml. The treatment of HepG2 cells with 100 µg/mlB. elliptica and B. ilicifolia extracts revealed a higher glucose uptake of 121 percent and 123 percent respectively when compared to the pharmaceutical drug berberine. However, both plants extracts displayed weak significant glucose uptake in L6 muscle cell line at all the tested concentrations when compared to insulin at 6 μg/ml. The crude extracts of both plants demonstrated no significant effect on 3T3-L1 lipid accumulation and INS-1 glucose metabolism. The extracts of both plants strongly reduced NO production in RAW macrophages at the highest concentration (100 μg/ml) tested in this study, which at least in part, may be explained by the flavanoids and phenols content of these plants. In addition, the crude extracts of both plants displayed weak significant inhibition on the CYP3A4 activity indicating that both plant extracts do not interfere with the cytochrome P450 activation or metabolism of anti-diabetic drugs, that may be taken together with the plant extracts. In conclusion, the results obtained from this study revealed that both plants could possibly exert their hypoglycemic effect via interactions with insulin receptor, thereby enhancing glucose metabolism as well as maintaining and repairing the health state and function of the pancreatic beta cells. The findings in this study, therefore provide evidence that B. elliptica and B. ilicifolia possess antidiabetic properties, and support their folkloric use for the management of diabetes. However, due to the potential toxicity of both plant extracts, they must be prescribed with caution.
- Full Text:
- Authors: Sagbo, Idowu Jonas
- Date: 2017
- Subjects: Herbs -- Therapeutic use Pharmacognosy Diabetes
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10353/8154 , vital:31624
- Description: International Diabetes Federation (IDF) estimated that there are 415 million people globally that are suffering from diabetes and this figure is likely to double by 2040. In South Africa, the number of people suffering from diabetes is believed to be rising steadily and the current antidiabetic therapies include the use of expensive pharmaceutical drugs which after display numerous adverse side effects. Herbal medicine is an alternative treatment strategy used by many rural populations for the management of diabetes, which is cost-effective and with minimal reported side effects. The aim of this study was to validate the antidiabetic activity and to elucidate the possible mechanisms of action of the aqueous leaf extracts prepared from two plants, Brachylaena elliptica and Brachylaena ilicifolia, used by traditional healers in the Eastern Cape, South Africa for the treatment of diabetes. The current pharmaceutical drugs target site, including carbohydrate digestion, regulation of blood glucose levels through insulin levels or activating glucose uptake in liver and muscle. The plants extracts were therefore evaluated for their potential to mimic or replace these drugs. The inhibibitory effects on alpha amylase, alpha glucosidase, DPP-IV activity and pancreatic lipase were evaluated at various concentrations of the plant extracts. To evaluate the suitability of these extracts for cell based in vitro assay as well as to determine the safety of the plant extracts for human consumption, cytotoxicity towards HepG2 cells was assessed using the MTT assay. The effect of both plant extracts on glucose uptake in HepG2 and L6 cells, lipid accumulation in 3T3-L1 preadipocytes and NO production in RAW macrophages were investigated. Both plant extracts were also tested for INS-1 proliferation using the imageXpress®Micro XLS analysis. The MTT reduction assay was also used to investigate the effect of extracts on INS-1 glucose metabolism and as a reflection of insulin secretion. The inhibitory activity of the crude extracts of both plants on protein glycation, collagenase activity and CYP3A4 interaction were also evaluated The crude extracts of both plants displayed no significant inhibition on alpha amylase, alpha glucosidase, dipeptidyl peptidase-IV, lipase, protein glycation and collagenase compared to relevant standard inhibitors, acarbose, EGCG, Diprotin A, Orlistart and aminoguanidine. The mild inhibition of B. elliptica on alpha glucosidase activity at the highest concentration of the extract tested, was considered as not physiologically relevant. The aqueous extracts of bothplants showed < 40 percent cell death in HepG2 cells even at high concentration and hence support the use of the plants by traditional healers. The extracts of B. ellipticaat 25 µg/ml, displayed a proliferative effect on INS-1 cells when compared to the negative control and B. ilicifolia at 25 μg/ml. The treatment of HepG2 cells with 100 µg/mlB. elliptica and B. ilicifolia extracts revealed a higher glucose uptake of 121 percent and 123 percent respectively when compared to the pharmaceutical drug berberine. However, both plants extracts displayed weak significant glucose uptake in L6 muscle cell line at all the tested concentrations when compared to insulin at 6 μg/ml. The crude extracts of both plants demonstrated no significant effect on 3T3-L1 lipid accumulation and INS-1 glucose metabolism. The extracts of both plants strongly reduced NO production in RAW macrophages at the highest concentration (100 μg/ml) tested in this study, which at least in part, may be explained by the flavanoids and phenols content of these plants. In addition, the crude extracts of both plants displayed weak significant inhibition on the CYP3A4 activity indicating that both plant extracts do not interfere with the cytochrome P450 activation or metabolism of anti-diabetic drugs, that may be taken together with the plant extracts. In conclusion, the results obtained from this study revealed that both plants could possibly exert their hypoglycemic effect via interactions with insulin receptor, thereby enhancing glucose metabolism as well as maintaining and repairing the health state and function of the pancreatic beta cells. The findings in this study, therefore provide evidence that B. elliptica and B. ilicifolia possess antidiabetic properties, and support their folkloric use for the management of diabetes. However, due to the potential toxicity of both plant extracts, they must be prescribed with caution.
- Full Text:
Antidiabetic and toxicological properties of Dianthus thunbergii (Caryophyllaceae) roots and Hypoxis argentea (Hypoxidaceae) corms
- Authors: Akinrinde, Akinleye Stephen
- Date: 2017
- Subjects: Medicinal plants--South Africa--Eastern Cape Traditional medicine--South Africa--Eastern Cape Diabetics--Alternative treatment--South Africa
- Language: English
- Type: Thesis , Doctoral , Biochemistry
- Identifier: http://hdl.handle.net/10353/16036 , vital:40606
- Description: Diabetes mellitus is a chronic metabolic disorder primarily characterized by elevated blood glucose levels. Its rapidly increasing prevalence as a major non-communicable disease is now a growing concern for both developed and developing countries. The development of safe, cost-effective and pharmacologically-effective medications continues to a major challenge of antidiabetic management. Since most conventional antidiabetic drugs are costly and present with numerous adverse effects, alternatives are increasingly being sought from natural sources, most notably medicinal plants, as viable approaches to tackling the scourge of this disease. In the Eastern Cape Province of South Africa, the roots of Dianthus thunbergii and corms of Hypoxis argentea are frequently used traditionally for the treatment of diabetes mellitus, although no scientific evidence exists to validate their effectiveness for diabetes management. The studies conducted in the resent research were, therefore, aimed at investigating the antidiabetic and toxicological properties of these plants, in an attempt to providing data towards validating their use in traditional management of diabetes mellitus. Aqueous and ethanol extracts of the underground parts of D. thunbergii and H. argentea were initially subjected to analysis of their phytochemical composition, relative to standard compounds, and the nature of their in-vitro antioxidant activities using standard spectrophotometric methods. The potentials of these extracts for cytotoxicity and/or cell proliferation were evaluated using MTT assay in HepG2 cells and Crystal violet assay in INS-1 cells. These activities were further examined in INS-1 cells using live cell fluorescence imaging techniques. To evaluate the antidiabetic properties of the extracts, they were screened for their inhibitory effects on the activities of different enzymes including α-amylase, α-glucosidase, porcine pancreatic lipase, Dipeptidyl peptidase IV (DPP-IV), collagenase and the drug metabolizing enzyme, CYP3A4, while also assessing their effects on protein glycation using in- vitro visible and fluorescence spectrophotometric approaches. Cell culture procedures were carried out to evaluate the effects of the extracts on glucose utilization in HepG2 cells and L6 myotubes; nitric oxide production in RAW 264.7 macrophages; glucose metabolism in INS-1 cells, as well as triglyceride accumulation in 3T3-L1 pre-adipocytes. Furthermore, identification of compounds present in the aqueous and ethanol extracts was carried out by Liquid chromatography- Mass spectrometry (LC-MS), while volatile oils extracted from fresh and dried parts of the two plants by hydrodistillation were also analyzed by Gas chromatography-Mass spectrometry (GC-MS). The ethanol extracts of both D. thunbergii and H. argentea contained higher amounts of total phenols, flavonoids, tannins, proanthocyanidins and alkaloids, when compared with the aqueous extracts. This finding was in direct correlation with the antioxidant activities of the extracts, with the ethanol extracts of both plants demonstrating stronger scavenging activities against hydrogen peroxide, nitric oxide, ABTS and DPPH radicals, while also exhibiting higher ferric reducing antioxidant potentials, when compared with the aqueous extracts, and in some cases, the standard antioxidants, Vitamin C, butylated hydroxytoluene and rutin. The aqueous extracts of D. thunbergii exhibited the highest toxicity in HepG2 cells with IC50 < 50 μg/ml, while also producing a concentration-dependent reduction in the viability of INS-1 cells up to 41.81percent at 50 μg/ml. Both extracts of H. argentea, however, did not produce any significant toxicity in these cells. Fluorescence imaging of live INS-1 cells using Hoechst and propidium iodide staining revealed stimulation of cell proliferation by H. argentea, while the cytotoxicity of D. thunbergii was further confirmed. H. argentea caused stimulation of glucose uptake in HepG2 cells up to 119.58 percent at 100 μg/ml and as much as 116.96 percent in L6 myotubes at 50 μg/ml, without showing toxicity to these cells. D. thunbergii produced 18.39 percent increase in L6 glucose uptake above untreated control; although its effect on HepG2 glucose uptake was irrelevant as significant toxicity was produced in these cells. H. argentea produced a concentration-dependent reduction in nitric oxide production in RAW macrophages, although not as effectively as the positive control, aminoguanidine. Again, the toxicity of D. thunbergii to this cell line precludes the relevance of nitric oxide inhibition as an antidiabetic mechanism for this plant. D. thunbergii produced a concentration-dependent increase in 3T3-L1 triglyceride accumulation, as measured by Oil red O staining, compared to untreated cells, while H. argentea exerted no significant alterations in pre- adipocyte differentiation. Generally, the two plants produced weak inhibition of the activities of the various enzymes measured, suggesting that this mechanism may not play a major role in the activities of these plants as possible antidiabetic agents. GC-MS analysis revealed major differences in the volatile oil composition between fresh and dried plant parts for both plants. Most notably, total terpenoid content of D. thunbergii oils reduced significantly from 77.17 percent in the fresh root oil to 47.58 percent in the dried root oil. Total terpenoid content was much lower in H. argentea oils, but similarly reduced from 10.58 percent in the fresh corm oil to 4.00 percent in the dried corm oil. LC-MS analysis enabled the tentative identification of compounds including phenolic glycosides, flavonoids, alkaloids, terpenoids saponins and sapogenins, many of which have been reported in literature to exert bioactivities relevant to the ones elucidated in the present study. Overall, H. argentea exhibited antidiabetic properties that may be mediated by its stimulation of glucose uptake in HepG2 and L6 cells; stimulation of proliferation in INS-1 cells; lack of stimulation of 3T3-L1 triglyceride accumulation and a tendency to reduce nitric oxide production in RAW macrophages. These activities suggest that H. argentea has promise for further investigations as an antidiabetic agent. On the contrary, D. thunbergii exhibited significant toxicity to HepG2 cells, INS-1 cells and RAW macrophages. Its cytotoxicity at the concentrations investigated in the present studies raises significant concerns about any potential antidiabetic applications for this plant.
- Full Text:
- Authors: Akinrinde, Akinleye Stephen
- Date: 2017
- Subjects: Medicinal plants--South Africa--Eastern Cape Traditional medicine--South Africa--Eastern Cape Diabetics--Alternative treatment--South Africa
- Language: English
- Type: Thesis , Doctoral , Biochemistry
- Identifier: http://hdl.handle.net/10353/16036 , vital:40606
- Description: Diabetes mellitus is a chronic metabolic disorder primarily characterized by elevated blood glucose levels. Its rapidly increasing prevalence as a major non-communicable disease is now a growing concern for both developed and developing countries. The development of safe, cost-effective and pharmacologically-effective medications continues to a major challenge of antidiabetic management. Since most conventional antidiabetic drugs are costly and present with numerous adverse effects, alternatives are increasingly being sought from natural sources, most notably medicinal plants, as viable approaches to tackling the scourge of this disease. In the Eastern Cape Province of South Africa, the roots of Dianthus thunbergii and corms of Hypoxis argentea are frequently used traditionally for the treatment of diabetes mellitus, although no scientific evidence exists to validate their effectiveness for diabetes management. The studies conducted in the resent research were, therefore, aimed at investigating the antidiabetic and toxicological properties of these plants, in an attempt to providing data towards validating their use in traditional management of diabetes mellitus. Aqueous and ethanol extracts of the underground parts of D. thunbergii and H. argentea were initially subjected to analysis of their phytochemical composition, relative to standard compounds, and the nature of their in-vitro antioxidant activities using standard spectrophotometric methods. The potentials of these extracts for cytotoxicity and/or cell proliferation were evaluated using MTT assay in HepG2 cells and Crystal violet assay in INS-1 cells. These activities were further examined in INS-1 cells using live cell fluorescence imaging techniques. To evaluate the antidiabetic properties of the extracts, they were screened for their inhibitory effects on the activities of different enzymes including α-amylase, α-glucosidase, porcine pancreatic lipase, Dipeptidyl peptidase IV (DPP-IV), collagenase and the drug metabolizing enzyme, CYP3A4, while also assessing their effects on protein glycation using in- vitro visible and fluorescence spectrophotometric approaches. Cell culture procedures were carried out to evaluate the effects of the extracts on glucose utilization in HepG2 cells and L6 myotubes; nitric oxide production in RAW 264.7 macrophages; glucose metabolism in INS-1 cells, as well as triglyceride accumulation in 3T3-L1 pre-adipocytes. Furthermore, identification of compounds present in the aqueous and ethanol extracts was carried out by Liquid chromatography- Mass spectrometry (LC-MS), while volatile oils extracted from fresh and dried parts of the two plants by hydrodistillation were also analyzed by Gas chromatography-Mass spectrometry (GC-MS). The ethanol extracts of both D. thunbergii and H. argentea contained higher amounts of total phenols, flavonoids, tannins, proanthocyanidins and alkaloids, when compared with the aqueous extracts. This finding was in direct correlation with the antioxidant activities of the extracts, with the ethanol extracts of both plants demonstrating stronger scavenging activities against hydrogen peroxide, nitric oxide, ABTS and DPPH radicals, while also exhibiting higher ferric reducing antioxidant potentials, when compared with the aqueous extracts, and in some cases, the standard antioxidants, Vitamin C, butylated hydroxytoluene and rutin. The aqueous extracts of D. thunbergii exhibited the highest toxicity in HepG2 cells with IC50 < 50 μg/ml, while also producing a concentration-dependent reduction in the viability of INS-1 cells up to 41.81percent at 50 μg/ml. Both extracts of H. argentea, however, did not produce any significant toxicity in these cells. Fluorescence imaging of live INS-1 cells using Hoechst and propidium iodide staining revealed stimulation of cell proliferation by H. argentea, while the cytotoxicity of D. thunbergii was further confirmed. H. argentea caused stimulation of glucose uptake in HepG2 cells up to 119.58 percent at 100 μg/ml and as much as 116.96 percent in L6 myotubes at 50 μg/ml, without showing toxicity to these cells. D. thunbergii produced 18.39 percent increase in L6 glucose uptake above untreated control; although its effect on HepG2 glucose uptake was irrelevant as significant toxicity was produced in these cells. H. argentea produced a concentration-dependent reduction in nitric oxide production in RAW macrophages, although not as effectively as the positive control, aminoguanidine. Again, the toxicity of D. thunbergii to this cell line precludes the relevance of nitric oxide inhibition as an antidiabetic mechanism for this plant. D. thunbergii produced a concentration-dependent increase in 3T3-L1 triglyceride accumulation, as measured by Oil red O staining, compared to untreated cells, while H. argentea exerted no significant alterations in pre- adipocyte differentiation. Generally, the two plants produced weak inhibition of the activities of the various enzymes measured, suggesting that this mechanism may not play a major role in the activities of these plants as possible antidiabetic agents. GC-MS analysis revealed major differences in the volatile oil composition between fresh and dried plant parts for both plants. Most notably, total terpenoid content of D. thunbergii oils reduced significantly from 77.17 percent in the fresh root oil to 47.58 percent in the dried root oil. Total terpenoid content was much lower in H. argentea oils, but similarly reduced from 10.58 percent in the fresh corm oil to 4.00 percent in the dried corm oil. LC-MS analysis enabled the tentative identification of compounds including phenolic glycosides, flavonoids, alkaloids, terpenoids saponins and sapogenins, many of which have been reported in literature to exert bioactivities relevant to the ones elucidated in the present study. Overall, H. argentea exhibited antidiabetic properties that may be mediated by its stimulation of glucose uptake in HepG2 and L6 cells; stimulation of proliferation in INS-1 cells; lack of stimulation of 3T3-L1 triglyceride accumulation and a tendency to reduce nitric oxide production in RAW macrophages. These activities suggest that H. argentea has promise for further investigations as an antidiabetic agent. On the contrary, D. thunbergii exhibited significant toxicity to HepG2 cells, INS-1 cells and RAW macrophages. Its cytotoxicity at the concentrations investigated in the present studies raises significant concerns about any potential antidiabetic applications for this plant.
- Full Text:
Applicability of putative probiotic bacteria to replace antibiotics as growth promoters in commercial and indigenous piglets
- Authors: Dlamini, Ziyanda Confidence
- Date: 2017
- Subjects: Antibiotics , Dietary supplements , Piglets
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/11428 , vital:39071
- Description: Antibiotic use in pig production contributes to development of antimicrobial resistance in food animals and risk of antibiotic residues in animal food products. The use of probiotics appears to be the potential alternative to antibiotics. This work is designed to validate the applicability of administered direct-fed putative probiotic strains of Lactobacillus reuteri ZJ625, Lactobacillus reuteri VB4, Lactobacillus salivarius ZJ614 and Streptococcus salivarius NBCR 13956 on growth performance, hematological parameters, and antibody stimulation in weaned commercial and indigenous pigs. Forty-five weaned piglets were divided into five treatment experimental groups as follows: Diet with: antibiotic (PC), No antibiotic and no probiotic (NC), Probiotic (P1) (P2) combination of probiotics (P3). Piglets’ performance were monitored during the trial. Faecal and ileum samples were collected for microbial count analysis. Blood samples were collected from the sacrificed piglets at the end of the trial, for the hematological and biochemical analysis and probiotics ability to stimulate immunoglobulin G (IgG). Four probiotic strains were tested for cell surface hydrophobicity against xylene, chloroform and ethyl acetate. The strains were also tested for auto-aggregation and co-aggregation abilities. Enterococcus faecalis ATCC 29212 was used as a control pathogen in the co-aggregation ability test. The probiotic strains were also screened for presence of virulence and resistance genes. DNA was extracted from all the four probiotic strains using ZR Fungal/Bacterial DNA MiniPepTM (Zymo Research, USA) following manufacturer’s instructions. The DNA samples were later amplified in PCR reactions with specific primers to detect virulence genes of adhesion collagen protein (ace), aggregation substances (agg and asa); antibiotic resistance genes of: Vancomycin vanA, Vancomycin vanC1 and Vancomycin vanC2/3. The data was analyzed by one-way ANOVA using SAS statistics software (SAS 9.3) (2003). Results from the study revealed that supplementation of probiotics had no effect on feed intake (FI) in all of the experimental groups. However, supplementation of probiotics in P3 treatment resulted in greater average daily gain (ADG) and improved feed conversion ratio (FCR) of weaned piglets (p < 0.05). Microbial count of fecal samples from all the treatment groups did not differ while ileum samples had lower enteric bacteria in P3 group as compared to other treatments. Concentration of albumin, globulin, neutrophils and basophils were high in NC treatment compared to other treatments. (p < 0.05). IgG concentration was highest in P3 compared to other treatment (p < 0.05). All probiotics strains showed high hydrophobicity to all solvents used in the test. Cell Surface Hydrophobic was highest in xylene (78percent – 84percent), followed by chloroform (68percent – 75percent) and lowest in ethyl acetate (52percent - 60percent). All of the test strains showed high auto-aggregation ability after 4 hr of incubation. L .reuteri VB4 exhibited highest auto-aggregation ability of 70percent, while the least auto-aggregation ability of 60percent was observed for L. salivarius ZJ614. In the co-aggregation assays, all probiotic bacterial strains exhibited a strong co-aggregation of 45percent to 56percent after 4 hr of incubation at 37 °C. L. reuteri VB4 exhibited highest co-aggregation ability while L. salivarius ZJ614 exhibited the lowest co-aggregation ability. No presence of virulence genes ace and asa was detected in all of the putative probiotic strains, but agg virulence gene was detected in L. reuteri VB4 strains. L. reuteri VB4 and S. salivarius NBRC13956 had resistance genes vanC 2/3 and vanC1, respectively. Overall, the results from this study, suggest that the candidate probiotics strains have enhanced the growth and measured blood parameters of the weaned piglets used in these experiments, and also, the probiotic strains have the abilities to initiate the stimulation of IgG. Probiotics could be used as a novel alternative to antibiotics in the animal nutrition and production, with the abilities to confer health benefits and promote growth. The outcome of the research advocates that these probiotics will be beneficial to pig production, and might outcompete the current antibiotics in use as growth promoters. One of the major advantages of probiotics to pig industry is the production of antibiotic free and good quality pork meat to local and international consumers, thus, enhancing South African Pork industry.
- Full Text:
- Authors: Dlamini, Ziyanda Confidence
- Date: 2017
- Subjects: Antibiotics , Dietary supplements , Piglets
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/11428 , vital:39071
- Description: Antibiotic use in pig production contributes to development of antimicrobial resistance in food animals and risk of antibiotic residues in animal food products. The use of probiotics appears to be the potential alternative to antibiotics. This work is designed to validate the applicability of administered direct-fed putative probiotic strains of Lactobacillus reuteri ZJ625, Lactobacillus reuteri VB4, Lactobacillus salivarius ZJ614 and Streptococcus salivarius NBCR 13956 on growth performance, hematological parameters, and antibody stimulation in weaned commercial and indigenous pigs. Forty-five weaned piglets were divided into five treatment experimental groups as follows: Diet with: antibiotic (PC), No antibiotic and no probiotic (NC), Probiotic (P1) (P2) combination of probiotics (P3). Piglets’ performance were monitored during the trial. Faecal and ileum samples were collected for microbial count analysis. Blood samples were collected from the sacrificed piglets at the end of the trial, for the hematological and biochemical analysis and probiotics ability to stimulate immunoglobulin G (IgG). Four probiotic strains were tested for cell surface hydrophobicity against xylene, chloroform and ethyl acetate. The strains were also tested for auto-aggregation and co-aggregation abilities. Enterococcus faecalis ATCC 29212 was used as a control pathogen in the co-aggregation ability test. The probiotic strains were also screened for presence of virulence and resistance genes. DNA was extracted from all the four probiotic strains using ZR Fungal/Bacterial DNA MiniPepTM (Zymo Research, USA) following manufacturer’s instructions. The DNA samples were later amplified in PCR reactions with specific primers to detect virulence genes of adhesion collagen protein (ace), aggregation substances (agg and asa); antibiotic resistance genes of: Vancomycin vanA, Vancomycin vanC1 and Vancomycin vanC2/3. The data was analyzed by one-way ANOVA using SAS statistics software (SAS 9.3) (2003). Results from the study revealed that supplementation of probiotics had no effect on feed intake (FI) in all of the experimental groups. However, supplementation of probiotics in P3 treatment resulted in greater average daily gain (ADG) and improved feed conversion ratio (FCR) of weaned piglets (p < 0.05). Microbial count of fecal samples from all the treatment groups did not differ while ileum samples had lower enteric bacteria in P3 group as compared to other treatments. Concentration of albumin, globulin, neutrophils and basophils were high in NC treatment compared to other treatments. (p < 0.05). IgG concentration was highest in P3 compared to other treatment (p < 0.05). All probiotics strains showed high hydrophobicity to all solvents used in the test. Cell Surface Hydrophobic was highest in xylene (78percent – 84percent), followed by chloroform (68percent – 75percent) and lowest in ethyl acetate (52percent - 60percent). All of the test strains showed high auto-aggregation ability after 4 hr of incubation. L .reuteri VB4 exhibited highest auto-aggregation ability of 70percent, while the least auto-aggregation ability of 60percent was observed for L. salivarius ZJ614. In the co-aggregation assays, all probiotic bacterial strains exhibited a strong co-aggregation of 45percent to 56percent after 4 hr of incubation at 37 °C. L. reuteri VB4 exhibited highest co-aggregation ability while L. salivarius ZJ614 exhibited the lowest co-aggregation ability. No presence of virulence genes ace and asa was detected in all of the putative probiotic strains, but agg virulence gene was detected in L. reuteri VB4 strains. L. reuteri VB4 and S. salivarius NBRC13956 had resistance genes vanC 2/3 and vanC1, respectively. Overall, the results from this study, suggest that the candidate probiotics strains have enhanced the growth and measured blood parameters of the weaned piglets used in these experiments, and also, the probiotic strains have the abilities to initiate the stimulation of IgG. Probiotics could be used as a novel alternative to antibiotics in the animal nutrition and production, with the abilities to confer health benefits and promote growth. The outcome of the research advocates that these probiotics will be beneficial to pig production, and might outcompete the current antibiotics in use as growth promoters. One of the major advantages of probiotics to pig industry is the production of antibiotic free and good quality pork meat to local and international consumers, thus, enhancing South African Pork industry.
- Full Text:
Appraisal of experimental performance and modelling of an on-farm dairy milk bulk cooler: Fort Hare Dairy Trust, South Africa
- Authors: Mhundwa, Russel
- Date: 2017
- Subjects: Milk -- Cooling Milk -- Cooling -- Equipment and supplies Milk tanks -- Specifications
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10353/4671 , vital:28487
- Description: South Africa contributes approximately 0.5 percent to the total world milk demand and is the third largest producer of fresh cow milk in Africa after Sudan and Kenya. In comparison to any other enterprise, the cost of milk production is influenced by numerous factors, that in turn affect the profitability of the farm enterprise; however one of such factors is high electricity cost. In this regard, there is need for efficient operation of the milk processing plant at all stages and at the same time maximising on product quality and minimising on the cost of production including energy. At the dairy farm, milk handling mainly commences as the milk leaves the cow udder at 35°C–37°C and must be cooled rapidly to a storage temperature of 4°C in a bid to stop microbial activity. The cooling of the milk can be done directly by the bulk milk cooler (BMC) from 37°C to the required storage temperature of 4°C or it can be done successively through pre-cooling. The process of pre-cooling involves the use of a heat exchanger where in most instances the plate heat exchanger (PHE) is used as the pre-cooler (PC) thereby leading to energy savings in a dairy facility. Cooling of milk involves significant amount of energy and it could account for about 20 percent of the total energy consumed on a farm. The aim of the research was to develop mathematical models that could be used to predict the electrical energy performance and capture the cooling saving of an on-farm direct expansion bulk milk cooler (DXBMC) during the milk cooling process. Accordingly, data acquisition system (DAS) was designed and built to accurately measure the power consumption of the BMC, temperature of raw milk, room temperature, temperature of cold water, relative humidity and ambient temperature. The volume of milk produced per day was extracted from the daily records on the farm. In addition, the temperature sensors were connected to a four channel HOBO data loggers which were configured to log at every five-minutes interval. The results were analysed and the mathematical models were developed using MATLAB. The statistical Toolbox in MATLAB was used to rank the predictors according to their weight of importance using the ReliefF Algorithm test. The results showed that on average, the daily electrical energy consumed by the BMC at the two milking times was higher during the peak period (127.82 kWh and 93.86 kWh) than the off-peak period (48.31 kWh and 43.23 kWh). On average, the electricity used for cooling of milk on the dairy farm was 17.06 kWh/m3 of milk. The average monthly electricity used per cow on the farm was 8.03 kWh/cow which translated to an average of 0.26 kWh/cow/day The average specific energy consumption of the cooling system per litre of milk cooled was 0.02 kWh/L and was almost constant throughout the whole period of monitoring. Furthermore, the BMC was able to cool 57.33 L/kWh during the off-peak period which increased by 7.7 percent to 62.13 L/kWh during the peak period. Furthermore, mathematical models represented as multiple linear regression (MLR) models were built and developed using the experimental data. The developed mathematical models had good agreement with the experimental data as evidenced by the correlation coefficients of 0.922 and 0.8995 along with 0.935 and 0.930. The ReliefF Algorithm test revealed that the volume of milk was the principal contributor to the energy consumption of the BMC for both the morning (AM) and afternoon (PM) milking period. The Relative Prediction Error (RPE) was used to evaluate the suitability of the developed models. In this light, the AM off-peak model had RPE of 18.54 percent while the PM off-peak model had 14.42 percent. In addition, the AM peak and PM peak models had RPE of 19.23 percent and 18.95 percent respectively. This suggested that the MLR models for the off-peak and peak milking periods (both AM and PM) had acceptable prediction accuracy since the RPE values were between 10 percent and 20 percent. The findings from the experimental study showed that the coefficient of performance (COP) of the AM milking period was higher (2.20) than that of the PM milking period of the BMC (1.93). Increase in the milk volume led to an increase in the COP such that the peak period with higher milk volumes recorded a high COP increase of 12.61 percent and 19.81 percent for the AM and PM milking periods respectively.
- Full Text:
- Authors: Mhundwa, Russel
- Date: 2017
- Subjects: Milk -- Cooling Milk -- Cooling -- Equipment and supplies Milk tanks -- Specifications
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10353/4671 , vital:28487
- Description: South Africa contributes approximately 0.5 percent to the total world milk demand and is the third largest producer of fresh cow milk in Africa after Sudan and Kenya. In comparison to any other enterprise, the cost of milk production is influenced by numerous factors, that in turn affect the profitability of the farm enterprise; however one of such factors is high electricity cost. In this regard, there is need for efficient operation of the milk processing plant at all stages and at the same time maximising on product quality and minimising on the cost of production including energy. At the dairy farm, milk handling mainly commences as the milk leaves the cow udder at 35°C–37°C and must be cooled rapidly to a storage temperature of 4°C in a bid to stop microbial activity. The cooling of the milk can be done directly by the bulk milk cooler (BMC) from 37°C to the required storage temperature of 4°C or it can be done successively through pre-cooling. The process of pre-cooling involves the use of a heat exchanger where in most instances the plate heat exchanger (PHE) is used as the pre-cooler (PC) thereby leading to energy savings in a dairy facility. Cooling of milk involves significant amount of energy and it could account for about 20 percent of the total energy consumed on a farm. The aim of the research was to develop mathematical models that could be used to predict the electrical energy performance and capture the cooling saving of an on-farm direct expansion bulk milk cooler (DXBMC) during the milk cooling process. Accordingly, data acquisition system (DAS) was designed and built to accurately measure the power consumption of the BMC, temperature of raw milk, room temperature, temperature of cold water, relative humidity and ambient temperature. The volume of milk produced per day was extracted from the daily records on the farm. In addition, the temperature sensors were connected to a four channel HOBO data loggers which were configured to log at every five-minutes interval. The results were analysed and the mathematical models were developed using MATLAB. The statistical Toolbox in MATLAB was used to rank the predictors according to their weight of importance using the ReliefF Algorithm test. The results showed that on average, the daily electrical energy consumed by the BMC at the two milking times was higher during the peak period (127.82 kWh and 93.86 kWh) than the off-peak period (48.31 kWh and 43.23 kWh). On average, the electricity used for cooling of milk on the dairy farm was 17.06 kWh/m3 of milk. The average monthly electricity used per cow on the farm was 8.03 kWh/cow which translated to an average of 0.26 kWh/cow/day The average specific energy consumption of the cooling system per litre of milk cooled was 0.02 kWh/L and was almost constant throughout the whole period of monitoring. Furthermore, the BMC was able to cool 57.33 L/kWh during the off-peak period which increased by 7.7 percent to 62.13 L/kWh during the peak period. Furthermore, mathematical models represented as multiple linear regression (MLR) models were built and developed using the experimental data. The developed mathematical models had good agreement with the experimental data as evidenced by the correlation coefficients of 0.922 and 0.8995 along with 0.935 and 0.930. The ReliefF Algorithm test revealed that the volume of milk was the principal contributor to the energy consumption of the BMC for both the morning (AM) and afternoon (PM) milking period. The Relative Prediction Error (RPE) was used to evaluate the suitability of the developed models. In this light, the AM off-peak model had RPE of 18.54 percent while the PM off-peak model had 14.42 percent. In addition, the AM peak and PM peak models had RPE of 19.23 percent and 18.95 percent respectively. This suggested that the MLR models for the off-peak and peak milking periods (both AM and PM) had acceptable prediction accuracy since the RPE values were between 10 percent and 20 percent. The findings from the experimental study showed that the coefficient of performance (COP) of the AM milking period was higher (2.20) than that of the PM milking period of the BMC (1.93). Increase in the milk volume led to an increase in the COP such that the peak period with higher milk volumes recorded a high COP increase of 12.61 percent and 19.81 percent for the AM and PM milking periods respectively.
- Full Text:
Aspects of the feeding biology of the copepod Pseudodiaptomus hessei (Copepoda: Calanoida) under culture conditions
- Authors: Mzozo, Ziyanda
- Date: 2017
- Subjects: Copepoda Microbial ecology Fishes -- Feeding and feeds
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/4727 , vital:28502
- Description: This study aimed to investigate the feeding behaviour of the calanoid copepod, Pseudodiaptomus hessei, particularly whether it prefers to feed on a planktonic or benthic food source. The effect of different microalgae species on the feeding preference, ingestion, and gut evacuation rate were investigated. Two microalgae species (Isochrysis galbana and Tetraselmis suecica) were used, to test gut fullness and gut evacuation of P. hessei. The copepods were starved for 6 h and allowed to feed on monalgae diets, I .galbana and T. suecica, and a 1:1 binary diet mixture of T. suecica and I. galbana. The gut fullness and gut evacuation rates were determined by observing under the dissecting microscope and gut content percentage was recorded (as 0, 25, 50, 75 or 100 percent) along with copepod sex. Copepods that feed on T. suecica fill their gut after 30 min, while those that feed on I. galbana do so after 40 min. There was a significant difference (p= 0.02) between sexes with males filling their gut faster than females. However, copepods that fed on I. galbana evacuated their gut content completely after 30 min, while those fed on T suecica evacuated theirs after 40 min. There was no difference between males and females for gut evacuation. Even though there was high gut fullness rate when P. hessei was feeding on T. suecica, I. galbana is recommended as the best mono-algae diet to culture P. hessei. The feeding behaviour of P. hessei was investigated, as to whether it prefers to feed on a planktonic or benthic food source. Copepods were starved for 3 h and then allowed to feed on two microalgae species (I. galbana and T. suecica), one presented as a benthic and other as planktonic. Controls included swapping the modes of presentation for each algae species. Ingested microalgae species were quantified using real-time PCR, with threshold cycles interpreted as relative abundance ratio (planktonic microalgae divided by benthic microalgae species). Pseudodiaptomus hessei preferred to feed on planktonic food sources initially as there was a difference between planktonic and benthic ingested food (p=0.026). Copepods shifted from planktonic to benthic food sources after 80 min of feeding planktonically. Planktonic food sources were the preferred choice for P. hessei regardless of microalgae species offered. The results from this study also confirm that P. hessei feed on a benthic food source during the day under culture conditions. This information can be used as baseline information for aquaculturists to rear the species as live feed for marine fish larvae.
- Full Text:
- Authors: Mzozo, Ziyanda
- Date: 2017
- Subjects: Copepoda Microbial ecology Fishes -- Feeding and feeds
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/4727 , vital:28502
- Description: This study aimed to investigate the feeding behaviour of the calanoid copepod, Pseudodiaptomus hessei, particularly whether it prefers to feed on a planktonic or benthic food source. The effect of different microalgae species on the feeding preference, ingestion, and gut evacuation rate were investigated. Two microalgae species (Isochrysis galbana and Tetraselmis suecica) were used, to test gut fullness and gut evacuation of P. hessei. The copepods were starved for 6 h and allowed to feed on monalgae diets, I .galbana and T. suecica, and a 1:1 binary diet mixture of T. suecica and I. galbana. The gut fullness and gut evacuation rates were determined by observing under the dissecting microscope and gut content percentage was recorded (as 0, 25, 50, 75 or 100 percent) along with copepod sex. Copepods that feed on T. suecica fill their gut after 30 min, while those that feed on I. galbana do so after 40 min. There was a significant difference (p= 0.02) between sexes with males filling their gut faster than females. However, copepods that fed on I. galbana evacuated their gut content completely after 30 min, while those fed on T suecica evacuated theirs after 40 min. There was no difference between males and females for gut evacuation. Even though there was high gut fullness rate when P. hessei was feeding on T. suecica, I. galbana is recommended as the best mono-algae diet to culture P. hessei. The feeding behaviour of P. hessei was investigated, as to whether it prefers to feed on a planktonic or benthic food source. Copepods were starved for 3 h and then allowed to feed on two microalgae species (I. galbana and T. suecica), one presented as a benthic and other as planktonic. Controls included swapping the modes of presentation for each algae species. Ingested microalgae species were quantified using real-time PCR, with threshold cycles interpreted as relative abundance ratio (planktonic microalgae divided by benthic microalgae species). Pseudodiaptomus hessei preferred to feed on planktonic food sources initially as there was a difference between planktonic and benthic ingested food (p=0.026). Copepods shifted from planktonic to benthic food sources after 80 min of feeding planktonically. Planktonic food sources were the preferred choice for P. hessei regardless of microalgae species offered. The results from this study also confirm that P. hessei feed on a benthic food source during the day under culture conditions. This information can be used as baseline information for aquaculturists to rear the species as live feed for marine fish larvae.
- Full Text:
Assessing the role of the community radio stations in facilitating development : a case study of Forte FM in the Eastern Cape Province
- Authors: Mawokomayi, Betina
- Date: 2017
- Subjects: Radio in community development -- South Africa -- Eastern Cape Community radio -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSoc
- Identifier: http://hdl.handle.net/10353/5462 , vital:29259
- Description: Community radio stations play an important role in the development of their communities. Forte FM is a community radio station located at University of Fort Hare Alice campus and was established to facilitate development in communities within the Amathole district municipality. Alice, a small rural town under Raymond Mhlaba Municipality, is one of the communities under the coverage of Forte FM. This study assessed the role that Forte FM plays in facilitating the development of Alice community. The study adopted a mixed method design in which questionnaires and interviews were used to collect primary data. The study findings show that although Forte FM is faced with serious financial challenges, it contributes to the development of Alice in various ways which includes providing the community with agricultural information, health information, promoting local culture, equipping the community with skills, promote local artists and sports personnel and educational programmes. The findings also revealed that the community is involved in the management of some aspects of the station’s operations through a board which is elected annually by the community itself. The results indicate that the radio station involve some community members in programming as experts on some topics or as people who have experienced an ordeal so they could share their experiences and advice other people how to manage similar situations. However, there is need to involve the community in the whole production process. The respondents’ attributed poor levels of community participation in programming to insufficient funds. The study recommends that Forte FM should lobby members of the community to fund the production of programmes since the station belongs to the community. This will enhance the sense of community ownership towards the radio station.
- Full Text:
- Authors: Mawokomayi, Betina
- Date: 2017
- Subjects: Radio in community development -- South Africa -- Eastern Cape Community radio -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSoc
- Identifier: http://hdl.handle.net/10353/5462 , vital:29259
- Description: Community radio stations play an important role in the development of their communities. Forte FM is a community radio station located at University of Fort Hare Alice campus and was established to facilitate development in communities within the Amathole district municipality. Alice, a small rural town under Raymond Mhlaba Municipality, is one of the communities under the coverage of Forte FM. This study assessed the role that Forte FM plays in facilitating the development of Alice community. The study adopted a mixed method design in which questionnaires and interviews were used to collect primary data. The study findings show that although Forte FM is faced with serious financial challenges, it contributes to the development of Alice in various ways which includes providing the community with agricultural information, health information, promoting local culture, equipping the community with skills, promote local artists and sports personnel and educational programmes. The findings also revealed that the community is involved in the management of some aspects of the station’s operations through a board which is elected annually by the community itself. The results indicate that the radio station involve some community members in programming as experts on some topics or as people who have experienced an ordeal so they could share their experiences and advice other people how to manage similar situations. However, there is need to involve the community in the whole production process. The respondents’ attributed poor levels of community participation in programming to insufficient funds. The study recommends that Forte FM should lobby members of the community to fund the production of programmes since the station belongs to the community. This will enhance the sense of community ownership towards the radio station.
- Full Text:
Assessment of anti-urease and consequential inhibitory potential of South African honey extracts on the multiplication of drug-resistant, vacA and cagA positive helicobacter pylori strains under acidic conditions
- Authors: Dube, Callote
- Date: 2017
- Subjects: Helicobacter pylori Drug resistance in microorganisms Honey -- Therapeutic use
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10353/7787 , vital:30764
- Description: Helicobacter pylori, a neutralophile chronically infects the gastric stomach of more than half of the world’s population. Infection with the organism is associated with acute or chronic duodenal/gastric ulcer disease, gastritis, gastric adenocarcinoma, mucosa-associated tissue lymphoma (MALT) and primary B-cell gastric lymphoma and has been grouped as a class one carcinogen by the World Health Organisation (WHO). Prevalence of this organism is very high in developing countries especially in Africa, including South Africa. H. pylori treatment using the common first and second line regimens, triple therapy with two antibiotics and a proton pump inhibitor (PPI) is showing inefficiency due to increasing drug resistance. However, newly developed treatment regimens seem to be more expensive and are accompanied by more side effects. Honey contains phytochemicals which are a wealthy source of biologically active compounds some of which have been put into good use in the pharmaceutical industry. Pathogenesis of H. pylori infection in the human stomach relies on several virulence factors which include the urease enzyme, cagA and vacA. The urease enzyme actively hydrolyses urea to produce ammonia an important by-product involved in pH regulation favouring the survival of the organism in the acidic human stomach. This study therefore focuses on screening for anti-urease solvent extracts of South African honey, and evaluate whether inhibition of urease offsets the growth of H. pylori under acidic conditions. Locally produced natural honeys; Bush honey, Raw honey, Gold Crest honey, Q Bee honey, Little Bee honey, Fleures honey-radurised, Siyakholwa pure honey and Manuka honey; an import from New Zealand were purchased and the method by Syazana et al. (2010) was used for the extraction of compounds in honey. A standard strain ATCC 43526 (American Type Culture Collection, Manassas, VA, USA) and 48 pure cultures obtained from clinical isolates cultured from gastric corpus biopsy specimen of patients with gastric morbidities who were ix visiting the endoscopy unit in Livingstone Hospital, Port Elizabeth between June 2008 to December 2008 were initially used as source of urease enzyme as per extraction method done by Amin et al. (2013), but with modifications. Prior to urease extraction, H. pylori strains were identified by biochemical tests (urease, catalase, oxidase, Gram stain), confirmed by PCR targeting the glmM gene (140 bp) and drug resistance profiling was done on all the 48 strains according to Seanego et al. (2012). The screening for anti-urease active compounds was done according to Kaltwasser et al. (1966), a method relying on the reduction of NADH in a coupled urease dehydrogenase (GDH) system. Acetohydroxamic acid was used as a standard inhibitor. Prevalence of cytotoxin-associated gene A (cagA) gene and vacuolating cytotoxin gene A (vacA) gene was determined among all 48 clinical samples. The standard strains of H. pylori, X47 (cagA positive), J99 (vacA s1m1) and Tx30a (s2m2) were used as positive controls. H. pylori’s growth was then monitored under acidic pH in a cocktail spiked with anti-urease compounds (test samples) and in a cocktail without anti-urease compounds (negative control). Acetohydroxamic acid was used as a standard urease inhibitor. H. pylori multiplication was monitored in Brain Heart Infusion Broth (BHIB) adjusted to pH of 2, 3, 4, 5, 6 and 7. The strain MP01 was used as a standard urease negative strain while X47 and J99 were used as positive standards for cagA and vacA s1m1 respectively. The compounds that had anti-urease activity and were successful towards suppressing the multiplication of H. pylori under acidic environment, all other factors optimised, were subjected to gas chromatograph mass spectrometry (GC-MS) and liquid chromatograph spectrometry (LCMS) to determine volatile compounds and drugs in honey extracts respectively. The findings of this study revealed that at a concentration of 50 mg/mL, urease inhibition by petroleum ether extracts of Gold Crest and Fleures honey, hexane extracts of Little Bee and Manuka honey, and chloroform extracts of Bush honey and Q Bee honey had a range above or equal to 50 percent and there was no significance difference in urease inhibition percentage (I percent) of urease from different sources including that extracted from drug resistant H. pylori (p >0.05). Virulence factors are important for the pathogenesis of H. pylori. All the 48 clinical isolates were glmM (140 bp) positive and cagA was detected in 97.9 percent of the test isolates. The vacA gene was detected in all isolates but with different subtypes. The vacA allelic combination s1m1 was detected in 75 percent of the test isolates and s1m2 allelic combination was detected in 16.7 percent of the test isolates while the combination s2m2 was detected in 8.3 percent of the test isolates. None of the test isolates possessed the allelic combination s2m1. When H. pylori multiplication was monitored under acidic conditions in the presence of anti-urease active compounds, it was revealed that anti-urease active compounds in honey are capable of inhibiting the normal multiplication of H. pylori strains that are cagA positive, vacA positive and drug resistant. The GC-MS analysis showed that Fleures honey (urease I percent = 67.8 – 68.5 percent) and Gold Crest honey (urease I percent = 50.9 percent – 53.3 percent), all petroleum ether extracts had 27 and 26 volatile compounds. The hexane extract of Manuka honey (urease I percent = 50.0 – 53.2) had 43 compounds detected. The chloroform extract of Q Bee (urease I percent = 64.2 – 66.2 percent) had 13 volatile compounds detected. All the volatile compounds considered as representative samples of GC-MS analysis had a spectral matching ≥ 90 percent with the NIST11 library. However, the majority of compounds that were detected by LC-MS in representative honey extracts include vardenafil, urapidil, hydrocortisone, e.t.c which are drugs commonly used in the treatment of different ailments or infections that affect human beings. In addition, two xi drugs, sulfaquinoxaline and hydroxyquinoline which are used in veterinary medicine and antiseptic, disinfectant and pesticide applications in agricultural activities were detected in Little Bee honey. We therefore conclude that inhibition of urease has a bactericidal effect on drug resistant, cagA positive and vacA positive H. pylori strains growing under acidic environment.
- Full Text:
- Authors: Dube, Callote
- Date: 2017
- Subjects: Helicobacter pylori Drug resistance in microorganisms Honey -- Therapeutic use
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10353/7787 , vital:30764
- Description: Helicobacter pylori, a neutralophile chronically infects the gastric stomach of more than half of the world’s population. Infection with the organism is associated with acute or chronic duodenal/gastric ulcer disease, gastritis, gastric adenocarcinoma, mucosa-associated tissue lymphoma (MALT) and primary B-cell gastric lymphoma and has been grouped as a class one carcinogen by the World Health Organisation (WHO). Prevalence of this organism is very high in developing countries especially in Africa, including South Africa. H. pylori treatment using the common first and second line regimens, triple therapy with two antibiotics and a proton pump inhibitor (PPI) is showing inefficiency due to increasing drug resistance. However, newly developed treatment regimens seem to be more expensive and are accompanied by more side effects. Honey contains phytochemicals which are a wealthy source of biologically active compounds some of which have been put into good use in the pharmaceutical industry. Pathogenesis of H. pylori infection in the human stomach relies on several virulence factors which include the urease enzyme, cagA and vacA. The urease enzyme actively hydrolyses urea to produce ammonia an important by-product involved in pH regulation favouring the survival of the organism in the acidic human stomach. This study therefore focuses on screening for anti-urease solvent extracts of South African honey, and evaluate whether inhibition of urease offsets the growth of H. pylori under acidic conditions. Locally produced natural honeys; Bush honey, Raw honey, Gold Crest honey, Q Bee honey, Little Bee honey, Fleures honey-radurised, Siyakholwa pure honey and Manuka honey; an import from New Zealand were purchased and the method by Syazana et al. (2010) was used for the extraction of compounds in honey. A standard strain ATCC 43526 (American Type Culture Collection, Manassas, VA, USA) and 48 pure cultures obtained from clinical isolates cultured from gastric corpus biopsy specimen of patients with gastric morbidities who were ix visiting the endoscopy unit in Livingstone Hospital, Port Elizabeth between June 2008 to December 2008 were initially used as source of urease enzyme as per extraction method done by Amin et al. (2013), but with modifications. Prior to urease extraction, H. pylori strains were identified by biochemical tests (urease, catalase, oxidase, Gram stain), confirmed by PCR targeting the glmM gene (140 bp) and drug resistance profiling was done on all the 48 strains according to Seanego et al. (2012). The screening for anti-urease active compounds was done according to Kaltwasser et al. (1966), a method relying on the reduction of NADH in a coupled urease dehydrogenase (GDH) system. Acetohydroxamic acid was used as a standard inhibitor. Prevalence of cytotoxin-associated gene A (cagA) gene and vacuolating cytotoxin gene A (vacA) gene was determined among all 48 clinical samples. The standard strains of H. pylori, X47 (cagA positive), J99 (vacA s1m1) and Tx30a (s2m2) were used as positive controls. H. pylori’s growth was then monitored under acidic pH in a cocktail spiked with anti-urease compounds (test samples) and in a cocktail without anti-urease compounds (negative control). Acetohydroxamic acid was used as a standard urease inhibitor. H. pylori multiplication was monitored in Brain Heart Infusion Broth (BHIB) adjusted to pH of 2, 3, 4, 5, 6 and 7. The strain MP01 was used as a standard urease negative strain while X47 and J99 were used as positive standards for cagA and vacA s1m1 respectively. The compounds that had anti-urease activity and were successful towards suppressing the multiplication of H. pylori under acidic environment, all other factors optimised, were subjected to gas chromatograph mass spectrometry (GC-MS) and liquid chromatograph spectrometry (LCMS) to determine volatile compounds and drugs in honey extracts respectively. The findings of this study revealed that at a concentration of 50 mg/mL, urease inhibition by petroleum ether extracts of Gold Crest and Fleures honey, hexane extracts of Little Bee and Manuka honey, and chloroform extracts of Bush honey and Q Bee honey had a range above or equal to 50 percent and there was no significance difference in urease inhibition percentage (I percent) of urease from different sources including that extracted from drug resistant H. pylori (p >0.05). Virulence factors are important for the pathogenesis of H. pylori. All the 48 clinical isolates were glmM (140 bp) positive and cagA was detected in 97.9 percent of the test isolates. The vacA gene was detected in all isolates but with different subtypes. The vacA allelic combination s1m1 was detected in 75 percent of the test isolates and s1m2 allelic combination was detected in 16.7 percent of the test isolates while the combination s2m2 was detected in 8.3 percent of the test isolates. None of the test isolates possessed the allelic combination s2m1. When H. pylori multiplication was monitored under acidic conditions in the presence of anti-urease active compounds, it was revealed that anti-urease active compounds in honey are capable of inhibiting the normal multiplication of H. pylori strains that are cagA positive, vacA positive and drug resistant. The GC-MS analysis showed that Fleures honey (urease I percent = 67.8 – 68.5 percent) and Gold Crest honey (urease I percent = 50.9 percent – 53.3 percent), all petroleum ether extracts had 27 and 26 volatile compounds. The hexane extract of Manuka honey (urease I percent = 50.0 – 53.2) had 43 compounds detected. The chloroform extract of Q Bee (urease I percent = 64.2 – 66.2 percent) had 13 volatile compounds detected. All the volatile compounds considered as representative samples of GC-MS analysis had a spectral matching ≥ 90 percent with the NIST11 library. However, the majority of compounds that were detected by LC-MS in representative honey extracts include vardenafil, urapidil, hydrocortisone, e.t.c which are drugs commonly used in the treatment of different ailments or infections that affect human beings. In addition, two xi drugs, sulfaquinoxaline and hydroxyquinoline which are used in veterinary medicine and antiseptic, disinfectant and pesticide applications in agricultural activities were detected in Little Bee honey. We therefore conclude that inhibition of urease has a bactericidal effect on drug resistant, cagA positive and vacA positive H. pylori strains growing under acidic environment.
- Full Text:
Assessment of national small and medium scale policy as a driver of local economic development : a case study of Ife Central Local government, Nigeria
- Authors: Adefemi, Adebayo Oluwaseun
- Date: 2017
- Subjects: Economic policy Economic development -- Nigeria
- Language: English
- Type: Thesis , Masters , M.Admin
- Identifier: http://hdl.handle.net/10353/12711 , vital:39301
- Description: This study, assessment of national small and medium scale policy as a driver of local economic development: a case study of Ife Central Local Government, Nigeria, was undertaken to find out if the National SME policy in Nigeria has performed its critical role of driving the local economic and if not, why, and also to identify measures to solve the problems that are hindering the implementation of the National SMEs policy. This study will therefore investigate the contributions of the Small and Medium Enterprises sub-sector of the Nigerian economy, its problems and prospects and also examine the National SME policy and the importance of the policy. The objective of this study is to explore the implementation of National SME policy as a driver of local economic development, analyse the challenges that are associated with implementing the National SME policy in Ife Central Local Government and make recommendations regarding the implementation of SME policy in Nigeria. A total of 5 MP’s were randomly selected from a cross section of 7 MP’s that represent Ife Central Local Government. Interviews was used to determine how the National SME policy is been implemented in there constituency and if they are facing any challenges as to the implementing the policy and how are they solving the problems that are associated with the policy. The major findings of this study include the following: the National SME policy is an important policy which if well implemented would help the development of SMEs and also have great impact on the local economic development. Among the problems associated with the policy according to the MP’s are financial problems, illiteracy level of people in their constituency, Land segmentation problem, marketing problems. This study therefore recommended that government should strengthen financial laws that mandates financial institutions to give low interest rate loans to SMEs with little or no collateral. The government should accelerated development and upgrade of rural roads and rail network and other infrastructural facilities and review taxies in favour of local manufacturers especially the SMEs.
- Full Text:
- Authors: Adefemi, Adebayo Oluwaseun
- Date: 2017
- Subjects: Economic policy Economic development -- Nigeria
- Language: English
- Type: Thesis , Masters , M.Admin
- Identifier: http://hdl.handle.net/10353/12711 , vital:39301
- Description: This study, assessment of national small and medium scale policy as a driver of local economic development: a case study of Ife Central Local Government, Nigeria, was undertaken to find out if the National SME policy in Nigeria has performed its critical role of driving the local economic and if not, why, and also to identify measures to solve the problems that are hindering the implementation of the National SMEs policy. This study will therefore investigate the contributions of the Small and Medium Enterprises sub-sector of the Nigerian economy, its problems and prospects and also examine the National SME policy and the importance of the policy. The objective of this study is to explore the implementation of National SME policy as a driver of local economic development, analyse the challenges that are associated with implementing the National SME policy in Ife Central Local Government and make recommendations regarding the implementation of SME policy in Nigeria. A total of 5 MP’s were randomly selected from a cross section of 7 MP’s that represent Ife Central Local Government. Interviews was used to determine how the National SME policy is been implemented in there constituency and if they are facing any challenges as to the implementing the policy and how are they solving the problems that are associated with the policy. The major findings of this study include the following: the National SME policy is an important policy which if well implemented would help the development of SMEs and also have great impact on the local economic development. Among the problems associated with the policy according to the MP’s are financial problems, illiteracy level of people in their constituency, Land segmentation problem, marketing problems. This study therefore recommended that government should strengthen financial laws that mandates financial institutions to give low interest rate loans to SMEs with little or no collateral. The government should accelerated development and upgrade of rural roads and rail network and other infrastructural facilities and review taxies in favour of local manufacturers especially the SMEs.
- Full Text: