An Immersive Audio Control System Using Mobile Devices and Ethernet AVB-Capable Speakers:
- Foss, Richard, Devonport, Sean
- Authors: Foss, Richard , Devonport, Sean
- Date: 2018
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/426745 , vital:72388 , https://www.aes.org/e-lib/browse.cfm?elib=19714
- Description: Immersive Sound is commonly used to create the localization of sound sources above, below, and around listeners. To achieve this immersive goal, sound systems are employing an ever-increasing number of speakers. Given that immersive sound systems have large speaker configurations such as in cinemas, theaters, museums, and home theater installations, there is a need to provide control for these various contexts and also to provide a means of automating this control. An immersive sound system has been created that allows for the real-time control over sound source localization. It is a multi-user client/server system where the client devices are mobile devices, thereby allowing remote control over sound source localization. Touch and orientation capabilities of mobile devices are used for the generation of three-dimensional coordinates. The server receives localization control messages from the client and uses an Ethernet AVB (audio video bridging) network to distribute appropriate mix levels to speakers with built-in signal processing. These localization messages can be recorded by users for later playback.
- Full Text:
- Date Issued: 2018
- Authors: Foss, Richard , Devonport, Sean
- Date: 2018
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/426745 , vital:72388 , https://www.aes.org/e-lib/browse.cfm?elib=19714
- Description: Immersive Sound is commonly used to create the localization of sound sources above, below, and around listeners. To achieve this immersive goal, sound systems are employing an ever-increasing number of speakers. Given that immersive sound systems have large speaker configurations such as in cinemas, theaters, museums, and home theater installations, there is a need to provide control for these various contexts and also to provide a means of automating this control. An immersive sound system has been created that allows for the real-time control over sound source localization. It is a multi-user client/server system where the client devices are mobile devices, thereby allowing remote control over sound source localization. Touch and orientation capabilities of mobile devices are used for the generation of three-dimensional coordinates. The server receives localization control messages from the client and uses an Ethernet AVB (audio video bridging) network to distribute appropriate mix levels to speakers with built-in signal processing. These localization messages can be recorded by users for later playback.
- Full Text:
- Date Issued: 2018
An information security governance model for industrial control systems
- Authors: Webster, Zynn
- Date: 2018
- Subjects: Computer networks -- Security measures , Data protection Computer security Business enterprises -- Computer networks -- Security measures
- Language: English
- Type: Thesis , Masters , MIT
- Identifier: http://hdl.handle.net/10948/36383 , vital:33934
- Description: Industrial Control Systems (ICS) is a term used to describe several types of control systems, including Supervisory Control and Data Acquisition (SCADA) systems, Distributed Control Systems (DCS) and Programmable Logic Controllers (PLC). These systems consist of a combination of control components (e.g. electrical, mechanical, pneumatic) which act together to achieve an industrial objective (e.g., manufacturing, transportation of matter or energy). ICS play a fundamental role in critical infrastructures such as electricity grids, oil, gas and manufacturing industries. Initially ICS had little resemblance to typical enterprise IT systems; they were isolated and running proprietary control protocols using specialized hardware and software. However, with initiatives such as Industry 4.0 and Industrial Internet of Things (IIoT), the nature of ICS has changed significantly. There is an ever-increasing use of commercial operating systems and standard protocols like TCP/IP and Ethernet. Consequently, modern ICS are more and more resembling conventional enterprise IT systems, and it is a well-known fact that these IT systems and networks are known to be vulnerable and that they require extensive management to ensure Confidentiality, Integrity, and Availability. Since ICS are now adopting conventional IT characteristics they are also accepting the associated risks. However, owing to the functional area of ICS, the consequences of these threats are much more severe than those of enterprise IT systems. The need to manage security for these systems with highly skilled IT personnel has become essential. Therefore, this research was focussed to identify which unique security controls for ICS and enterprise IT systems can be combined and/or tailored to provide the organization with a single set of comprehensive security controls. By doing an investigation on existing standards and best practices for both enterprise IT and ICS environments, this study has produced a single set of security controls and presented how the security controls can be integrated into an existing information security governance model which organizations can use as a basis for generating a security framework, used not only to secure their enterprise IT systems, but also including the security of their ICS.
- Full Text:
- Date Issued: 2018
- Authors: Webster, Zynn
- Date: 2018
- Subjects: Computer networks -- Security measures , Data protection Computer security Business enterprises -- Computer networks -- Security measures
- Language: English
- Type: Thesis , Masters , MIT
- Identifier: http://hdl.handle.net/10948/36383 , vital:33934
- Description: Industrial Control Systems (ICS) is a term used to describe several types of control systems, including Supervisory Control and Data Acquisition (SCADA) systems, Distributed Control Systems (DCS) and Programmable Logic Controllers (PLC). These systems consist of a combination of control components (e.g. electrical, mechanical, pneumatic) which act together to achieve an industrial objective (e.g., manufacturing, transportation of matter or energy). ICS play a fundamental role in critical infrastructures such as electricity grids, oil, gas and manufacturing industries. Initially ICS had little resemblance to typical enterprise IT systems; they were isolated and running proprietary control protocols using specialized hardware and software. However, with initiatives such as Industry 4.0 and Industrial Internet of Things (IIoT), the nature of ICS has changed significantly. There is an ever-increasing use of commercial operating systems and standard protocols like TCP/IP and Ethernet. Consequently, modern ICS are more and more resembling conventional enterprise IT systems, and it is a well-known fact that these IT systems and networks are known to be vulnerable and that they require extensive management to ensure Confidentiality, Integrity, and Availability. Since ICS are now adopting conventional IT characteristics they are also accepting the associated risks. However, owing to the functional area of ICS, the consequences of these threats are much more severe than those of enterprise IT systems. The need to manage security for these systems with highly skilled IT personnel has become essential. Therefore, this research was focussed to identify which unique security controls for ICS and enterprise IT systems can be combined and/or tailored to provide the organization with a single set of comprehensive security controls. By doing an investigation on existing standards and best practices for both enterprise IT and ICS environments, this study has produced a single set of security controls and presented how the security controls can be integrated into an existing information security governance model which organizations can use as a basis for generating a security framework, used not only to secure their enterprise IT systems, but also including the security of their ICS.
- Full Text:
- Date Issued: 2018
An institutional framework for expanding into Africa: a focus on global multinational companies
- Authors: Dipha, Lazola
- Date: 2018
- Subjects: International business enterprises -- Management , Globalization -- Economic aspects
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: http://hdl.handle.net/10948/29984 , vital:30804
- Description: Global multinational companies (MNCs) continue to face unique challenges in expanding into African emerging markets (AEMs). The biggest contributor to this is their lack of understanding and unwillingness to embrace the exceptional dynamics that exist in these countries, which has resulted in the majority of them defaulting to execute their own country’s domestic market strategies, resulting in failure to realise sustainable businesses. A misaligned and inappropriate strategy will fail dismally in terms of long-term sustainability of businesses. Urban & Hwindingwi (2016) took a diverse view in evaluating emerging markets, which will also be implemented in this study. They argued that “[they] see these features of emerging markets as symptoms of underlying market structures that share common, important and persistent differences from those in developed economies. Emerging markets reflect those transactional arenas where buyers and sellers are not easily or efficiently able to come together. The institutional voids make a market ‘emerging’ and are a prime source of the higher transaction costs and operating challenges in these markets”. “Market structures are the products of idiosyncratic historical, political, legal, economic and cultural forces within any country. All emerging markets feature insti-tutional voids, however, although the particular combination and severity of these voids varies from market to market”. In the previous years, corporate leaders and investors globally have pinned their hopes on the African growing story of promise becoming a reality. With a youthful, urbanising inhabitants, plentiful natural resources and a rising middle class; it looks like the continent has the correct components required for long-standing growth, possibly outshining the so-called tiger economies of East-Asia a generation ago. According to the McKinsey Global Institute titled Lions on the Move 2010 report; they forecasted consumer spending within the continent to grow by 40% and move GDPs by $1 trillion between 2008 to 2020. However, there are plenty multinationals that have become disheartened in their pursuit of operating in Africa.
- Full Text:
- Date Issued: 2018
- Authors: Dipha, Lazola
- Date: 2018
- Subjects: International business enterprises -- Management , Globalization -- Economic aspects
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: http://hdl.handle.net/10948/29984 , vital:30804
- Description: Global multinational companies (MNCs) continue to face unique challenges in expanding into African emerging markets (AEMs). The biggest contributor to this is their lack of understanding and unwillingness to embrace the exceptional dynamics that exist in these countries, which has resulted in the majority of them defaulting to execute their own country’s domestic market strategies, resulting in failure to realise sustainable businesses. A misaligned and inappropriate strategy will fail dismally in terms of long-term sustainability of businesses. Urban & Hwindingwi (2016) took a diverse view in evaluating emerging markets, which will also be implemented in this study. They argued that “[they] see these features of emerging markets as symptoms of underlying market structures that share common, important and persistent differences from those in developed economies. Emerging markets reflect those transactional arenas where buyers and sellers are not easily or efficiently able to come together. The institutional voids make a market ‘emerging’ and are a prime source of the higher transaction costs and operating challenges in these markets”. “Market structures are the products of idiosyncratic historical, political, legal, economic and cultural forces within any country. All emerging markets feature insti-tutional voids, however, although the particular combination and severity of these voids varies from market to market”. In the previous years, corporate leaders and investors globally have pinned their hopes on the African growing story of promise becoming a reality. With a youthful, urbanising inhabitants, plentiful natural resources and a rising middle class; it looks like the continent has the correct components required for long-standing growth, possibly outshining the so-called tiger economies of East-Asia a generation ago. According to the McKinsey Global Institute titled Lions on the Move 2010 report; they forecasted consumer spending within the continent to grow by 40% and move GDPs by $1 trillion between 2008 to 2020. However, there are plenty multinationals that have become disheartened in their pursuit of operating in Africa.
- Full Text:
- Date Issued: 2018
An integrated framework for assessing coastal community vulnerability across cultures, oceans and scales
- Aswani, Shankar, Howard, J A E, Gasalla, Maria A, Jennings, Sarah M, Malherbe, W, Martins, I M, Salim, Shyam S
- Authors: Aswani, Shankar , Howard, J A E , Gasalla, Maria A , Jennings, Sarah M , Malherbe, W , Martins, I M , Salim, Shyam S
- Date: 2018
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/123063 , vital:35402 , https://doi.10.1080/17565529.2018.1442795
- Description: Across the globe, many coastal communities rely on marine resources for their food security (FS), income and livelihoods and with predicted trends in human populations, the number of people reliant on these resources is likely to increase (FAO, 2012). However, the effects of climate change including increased variability are already being experienced by coastal communities and appear to be accelerating (Doney et al., 2012). Depending on a range of factors, including location, these changes are having mild to severe impacts on communities both in direct and indirect ways (Miller et al., 2010). Communities in coastal areas, for instance, are particularly at risk due to sea level rise but also through their dependence on marine resources that are impacted by multiple climate change pressures. A change in the availability and condition of marine resources has consequences on the livelihoods of fishing populations or those who depend directly on fishing as a source of food (Badjeck, Allison, Halls, & Dulvy, 2010).
- Full Text:
- Date Issued: 2018
- Authors: Aswani, Shankar , Howard, J A E , Gasalla, Maria A , Jennings, Sarah M , Malherbe, W , Martins, I M , Salim, Shyam S
- Date: 2018
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/123063 , vital:35402 , https://doi.10.1080/17565529.2018.1442795
- Description: Across the globe, many coastal communities rely on marine resources for their food security (FS), income and livelihoods and with predicted trends in human populations, the number of people reliant on these resources is likely to increase (FAO, 2012). However, the effects of climate change including increased variability are already being experienced by coastal communities and appear to be accelerating (Doney et al., 2012). Depending on a range of factors, including location, these changes are having mild to severe impacts on communities both in direct and indirect ways (Miller et al., 2010). Communities in coastal areas, for instance, are particularly at risk due to sea level rise but also through their dependence on marine resources that are impacted by multiple climate change pressures. A change in the availability and condition of marine resources has consequences on the livelihoods of fishing populations or those who depend directly on fishing as a source of food (Badjeck, Allison, Halls, & Dulvy, 2010).
- Full Text:
- Date Issued: 2018
An integrated talent-management approach for competitive advantage at a selected company
- Authors: Maxongo–Banzana, Ayanda
- Date: 2018
- Subjects: Employees -- Recruiting , Organizational effectiveness Personnel management Career development
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: http://hdl.handle.net/10948/31995 , vital:31907
- Description: This study aims to explore the concept of talent management that has received attention since a group of McKinsey Consultant coined the phrase “the War for Talent” in 1997. In the 21 century, businesses are continually confronted with pressure of aligning employee talent with broader organizational strategies. This research explores key interventions and activities in order to get a better understanding of best practices and approaches to managing talent. The specific contribution of this treatise is developing a talentship model to enable integrated approach to managing talent in order to achieve competitive advantage at Company X. Through a single case study approach, this study examines the current talent management approach at Company X, and the author develops an Integrated Talent management model, named “The Max- Banz Talentship House Model” to address key challenges confronting the company from a talent management perspective in order to achieve competitive advantage.
- Full Text:
- Date Issued: 2018
- Authors: Maxongo–Banzana, Ayanda
- Date: 2018
- Subjects: Employees -- Recruiting , Organizational effectiveness Personnel management Career development
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: http://hdl.handle.net/10948/31995 , vital:31907
- Description: This study aims to explore the concept of talent management that has received attention since a group of McKinsey Consultant coined the phrase “the War for Talent” in 1997. In the 21 century, businesses are continually confronted with pressure of aligning employee talent with broader organizational strategies. This research explores key interventions and activities in order to get a better understanding of best practices and approaches to managing talent. The specific contribution of this treatise is developing a talentship model to enable integrated approach to managing talent in order to achieve competitive advantage at Company X. Through a single case study approach, this study examines the current talent management approach at Company X, and the author develops an Integrated Talent management model, named “The Max- Banz Talentship House Model” to address key challenges confronting the company from a talent management perspective in order to achieve competitive advantage.
- Full Text:
- Date Issued: 2018
An interpretative phenomenological analysis of HIV positive individuals’ experiences of being in a support group
- Authors: Brink, Nicole
- Date: 2018
- Subjects: HIV-positive persons -- Social aspects Self-help groups AIDS (Disease) -- Patients -- Counseling of AIDS (Disease) -- Social aspects
- Language: English
- Type: text , Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10962/62988 , vital:28351
- Description: People who have been diagnosed HIV positive often experience distress and anxiety due to uncertainties pertaining to the implications of an HIV positive status. Research has shown that support groups have always been a way for people to cope with the distress and stressful circumstances associated with health conditions such as HIV. This research investigated the role of face to face support groups in the lives of those living with HIV. The primary focus of this research project is to provide an in-depth exploration of HIV positive individuals‟ experiences of being in a support group. The study aims to explore the positive and negative experiences of being in an HIV support group and aims to gain an understanding of the role support plays in the lives of those living with HIV. A qualitative research design was used to explore the above mentioned aim. Semi-structured interviews were conducted with five individuals, (three women and two men) who had experienced HIV support groups. The interviews were transcribed and then analysed according to the principles of interpretative phenomenological analysis (IPA). The findings of this study revealed five super-ordinate themes, which revealed not only the positive and negative experiences of being in a support group, but also the challenges that the participants‟ experienced before joining the group. Getting a sense of the participants‟ experience before joining the group allowed the researcher to get a better understanding of how useful or not the support group has been in helping them deal with the challenges of living with HIV. The themes included: „struggling to survive after diagnosis‟, „struggling to cope: adopting negative coping skills‟, „experiencing a turning-point: a will to survive‟, „attending support group: a sweet experience‟ and lastly, „attending support groups: a bitter experience‟. Findings suggest that for these participants, the advantages outweighed the disadvantages of being in a support group. Therefore this study suggests that face to face support groups are a viable and even necessary option for support. These findings support previous research and literature in regards to the importance of social support in the form of support groups in effectively assisting HIV positive people in their journey to adjust to the consequence of living with HIV.
- Full Text:
- Date Issued: 2018
- Authors: Brink, Nicole
- Date: 2018
- Subjects: HIV-positive persons -- Social aspects Self-help groups AIDS (Disease) -- Patients -- Counseling of AIDS (Disease) -- Social aspects
- Language: English
- Type: text , Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10962/62988 , vital:28351
- Description: People who have been diagnosed HIV positive often experience distress and anxiety due to uncertainties pertaining to the implications of an HIV positive status. Research has shown that support groups have always been a way for people to cope with the distress and stressful circumstances associated with health conditions such as HIV. This research investigated the role of face to face support groups in the lives of those living with HIV. The primary focus of this research project is to provide an in-depth exploration of HIV positive individuals‟ experiences of being in a support group. The study aims to explore the positive and negative experiences of being in an HIV support group and aims to gain an understanding of the role support plays in the lives of those living with HIV. A qualitative research design was used to explore the above mentioned aim. Semi-structured interviews were conducted with five individuals, (three women and two men) who had experienced HIV support groups. The interviews were transcribed and then analysed according to the principles of interpretative phenomenological analysis (IPA). The findings of this study revealed five super-ordinate themes, which revealed not only the positive and negative experiences of being in a support group, but also the challenges that the participants‟ experienced before joining the group. Getting a sense of the participants‟ experience before joining the group allowed the researcher to get a better understanding of how useful or not the support group has been in helping them deal with the challenges of living with HIV. The themes included: „struggling to survive after diagnosis‟, „struggling to cope: adopting negative coping skills‟, „experiencing a turning-point: a will to survive‟, „attending support group: a sweet experience‟ and lastly, „attending support groups: a bitter experience‟. Findings suggest that for these participants, the advantages outweighed the disadvantages of being in a support group. Therefore this study suggests that face to face support groups are a viable and even necessary option for support. These findings support previous research and literature in regards to the importance of social support in the form of support groups in effectively assisting HIV positive people in their journey to adjust to the consequence of living with HIV.
- Full Text:
- Date Issued: 2018
An intervention for high-risk type 2 diabetic clients during preconception and internatal in re-engineering of primary health care
- Authors: Ngoma, Siphokazi Amanda
- Date: 2018
- Subjects: Diabetes Diabetes Mellitus, Type 2
- Language: English
- Type: Thesis , Masters , (MCur) Nursing
- Identifier: http://hdl.handle.net/10353/10199 , vital:35375
- Description: The South African disease profile has changed significantly and has increasing prevalence of overweight and obesity in relation to body mass index. Type 2 diabetes mellitus is greatly associated with increased body mass index and poses a great health concern for women of childbearing age. Complications of type 2 diabetes mellitus in pregnancy has life-threatening for the mother and baby. South African women have a tendency of avoiding and neglecting routine screening before pregnancy, that is part of preconception care or internatal care. The aim of the study sought to identify women of childbearing age with predisposing factors of type 2 diabetes, in order to intervene, using a lifestyle modification to delay and prevent the occurrence of type 2 diabetes. A quantitative intervention study using parallel group randomized control trials was used. One hundred and forty-six women of childbearing age (18-45 years) were recruited and assigned to either control (n=73 + 9) or experimental group (n=73 +9). using a computer software randomizer application to avoid bias in selection of participants by the researcher. Both groups received health education from the researcher and the dietician from hospital. The control group had no intervention done, whereas the experimental group attended physical activity sessions which were administered by a hired lay coach. The findings showed that both groups are at risk of developing type 2 diabetes although they both groups lose weight. The weight loss of the control group was owed to education during recruitment. Vegetable consumption was minimal to the younger age group than the older age groups. Waist circumference above 105cm consumed less vegetables, a higher waist circumference is associated with insulin resistance. There was also minimal exercises and knowledge about the preconception care. Diabetes prevention is vital for the women of childbearing age as it affects both mother and child. The study recommends lifestyle modification (exercise and healthy diet) to prevent and delay of type 2 diabetes and gestational diabetes mellitus. The study also recommends Preconception care and Re- engineering of Primary health care uses community health care workers for screening high risk women during home visits.
- Full Text:
- Date Issued: 2018
- Authors: Ngoma, Siphokazi Amanda
- Date: 2018
- Subjects: Diabetes Diabetes Mellitus, Type 2
- Language: English
- Type: Thesis , Masters , (MCur) Nursing
- Identifier: http://hdl.handle.net/10353/10199 , vital:35375
- Description: The South African disease profile has changed significantly and has increasing prevalence of overweight and obesity in relation to body mass index. Type 2 diabetes mellitus is greatly associated with increased body mass index and poses a great health concern for women of childbearing age. Complications of type 2 diabetes mellitus in pregnancy has life-threatening for the mother and baby. South African women have a tendency of avoiding and neglecting routine screening before pregnancy, that is part of preconception care or internatal care. The aim of the study sought to identify women of childbearing age with predisposing factors of type 2 diabetes, in order to intervene, using a lifestyle modification to delay and prevent the occurrence of type 2 diabetes. A quantitative intervention study using parallel group randomized control trials was used. One hundred and forty-six women of childbearing age (18-45 years) were recruited and assigned to either control (n=73 + 9) or experimental group (n=73 +9). using a computer software randomizer application to avoid bias in selection of participants by the researcher. Both groups received health education from the researcher and the dietician from hospital. The control group had no intervention done, whereas the experimental group attended physical activity sessions which were administered by a hired lay coach. The findings showed that both groups are at risk of developing type 2 diabetes although they both groups lose weight. The weight loss of the control group was owed to education during recruitment. Vegetable consumption was minimal to the younger age group than the older age groups. Waist circumference above 105cm consumed less vegetables, a higher waist circumference is associated with insulin resistance. There was also minimal exercises and knowledge about the preconception care. Diabetes prevention is vital for the women of childbearing age as it affects both mother and child. The study recommends lifestyle modification (exercise and healthy diet) to prevent and delay of type 2 diabetes and gestational diabetes mellitus. The study also recommends Preconception care and Re- engineering of Primary health care uses community health care workers for screening high risk women during home visits.
- Full Text:
- Date Issued: 2018
An intervention study for low risk pregnant women and high risk pregnant women with GDM in Buffalo City Metropolitan Municipality, Eastern Cape, South Africa
- Authors: Vellem, Nonceba Mercy
- Date: 2018
- Subjects: Diabetes in pregnancy -- South Africa Nursing services -- South Africa -- Eastern Cape -- Administration Pregnancy -- Complications
- Language: English
- Type: Thesis , Doctoral , Nursing
- Identifier: http://hdl.handle.net/10353/9723 , vital:34891
- Description: Low risk and high risk pregnant women (HRPW) with gestational diabetes mellitus (GDM) who practise sedentary life style are at risk of overweight and obesity that predispose to gestational diabetes mellitus (GDM). The purpose of the study is to describe the effectiveness of an intervention strategy of modified diet and planned physical activity implemented amongst low-risk and high-risk pregnant women with GDM in Buffalo City Metropolitan Municipality in the Eastern Cape, South Africa. Quantitative experimental intervention design and stratified random sampling was used for drawing a sample of low-risk and high risk pregnant women with GDM. A sample size 291 of LRPW and n=34 HRPW were eligible for the study. The sample frame of the study was taken from the registers of the CHCs, Frere Hospital and Cecilia Makiwane Hospital (CMH). Questionnaires were used to collect data from the low and high risk pregnant with GDM. Descriptive statistics (frequency, percentages, mean and standard deviation) were used for categorical variables. A p-value of < 0.05 was considered statistically significant. The total analysed of LRPW were n=135 and HRPW n=34 all in control group due to miscarriages, phone loss and relocation. LRPW had some risk factors of overweight (6.2 percent), obesity (15.5 percent), family history of diabetes (16.5 percent). HRPW also had overweight and obesity of (14.7 percent) and family history of (26.4 percent) as the Pvalue was statistically significant of weight was 001, MUAC Pvalue of 000 and Pvalue of BMI 000. There were alarming abnormalities of urinalysis of protein, glucose, leucocytes and blood in urine. The LRPW and HRPW had consumption of high content of carbohydrates, vegetables, fats and low in fruit, vegetables and fibre diet. Both LRPW and HRPW were also interested on the education about modified diet benefits (95.1 percent) and modified diet programs networks (87.5 percent). The barriers showed that LRPW and HRPW were low motivation (90 percent) minimal knowledge and benefits of modified diet (93 percent) and socio-cultural issues (86 percent). The majority of participants both LRPW and HRPW showed the interest in the planned physical activity benefits especially frequent education (96.9 percent) and encouragement of social support networks (90.2 percent) and convenient location (89.4 percent). But the HRPW had lower engagement than the LRPW in planned physical activity and is associated with risk of GDM. Although pregnant women were faced with fatigue and low energy (98.6 percent), as pregnancy progresses, minimum information regarding benefits of planned physical activity (87.6 percent) and the socio-cultural influence (77.8 percent). The recommendations are education on frequent use of modified diet and planned physical activity that is available, less economical and within their context. Frequent screening of all pregnant women during pregnancy of BMI, MUAC and weight and educate accordingly. The availability of dietician and physical activity speciality is needed.
- Full Text:
- Date Issued: 2018
- Authors: Vellem, Nonceba Mercy
- Date: 2018
- Subjects: Diabetes in pregnancy -- South Africa Nursing services -- South Africa -- Eastern Cape -- Administration Pregnancy -- Complications
- Language: English
- Type: Thesis , Doctoral , Nursing
- Identifier: http://hdl.handle.net/10353/9723 , vital:34891
- Description: Low risk and high risk pregnant women (HRPW) with gestational diabetes mellitus (GDM) who practise sedentary life style are at risk of overweight and obesity that predispose to gestational diabetes mellitus (GDM). The purpose of the study is to describe the effectiveness of an intervention strategy of modified diet and planned physical activity implemented amongst low-risk and high-risk pregnant women with GDM in Buffalo City Metropolitan Municipality in the Eastern Cape, South Africa. Quantitative experimental intervention design and stratified random sampling was used for drawing a sample of low-risk and high risk pregnant women with GDM. A sample size 291 of LRPW and n=34 HRPW were eligible for the study. The sample frame of the study was taken from the registers of the CHCs, Frere Hospital and Cecilia Makiwane Hospital (CMH). Questionnaires were used to collect data from the low and high risk pregnant with GDM. Descriptive statistics (frequency, percentages, mean and standard deviation) were used for categorical variables. A p-value of < 0.05 was considered statistically significant. The total analysed of LRPW were n=135 and HRPW n=34 all in control group due to miscarriages, phone loss and relocation. LRPW had some risk factors of overweight (6.2 percent), obesity (15.5 percent), family history of diabetes (16.5 percent). HRPW also had overweight and obesity of (14.7 percent) and family history of (26.4 percent) as the Pvalue was statistically significant of weight was 001, MUAC Pvalue of 000 and Pvalue of BMI 000. There were alarming abnormalities of urinalysis of protein, glucose, leucocytes and blood in urine. The LRPW and HRPW had consumption of high content of carbohydrates, vegetables, fats and low in fruit, vegetables and fibre diet. Both LRPW and HRPW were also interested on the education about modified diet benefits (95.1 percent) and modified diet programs networks (87.5 percent). The barriers showed that LRPW and HRPW were low motivation (90 percent) minimal knowledge and benefits of modified diet (93 percent) and socio-cultural issues (86 percent). The majority of participants both LRPW and HRPW showed the interest in the planned physical activity benefits especially frequent education (96.9 percent) and encouragement of social support networks (90.2 percent) and convenient location (89.4 percent). But the HRPW had lower engagement than the LRPW in planned physical activity and is associated with risk of GDM. Although pregnant women were faced with fatigue and low energy (98.6 percent), as pregnancy progresses, minimum information regarding benefits of planned physical activity (87.6 percent) and the socio-cultural influence (77.8 percent). The recommendations are education on frequent use of modified diet and planned physical activity that is available, less economical and within their context. Frequent screening of all pregnant women during pregnancy of BMI, MUAC and weight and educate accordingly. The availability of dietician and physical activity speciality is needed.
- Full Text:
- Date Issued: 2018
An invastigation into factors that contribute to elder abuse: a case study of mount frère town in the umzimvubu local municipality
- Authors: Rasmeni, Nosabelo Lusanda
- Date: 2018
- Subjects: Older people -- Abuse of , Abused elderly -- Services for
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/19625 , vital:43153
- Description: The aim of this study was to explore factors which contribute to elder abuse in Mount Frere Town, in the Umzimvubu Local Municipality in the Eastern Cape Province. A qualitative approach was used to allow for the achievement of the research aim which was to explore contributory factors towards elderly abuse in Mount Frere Town. A purposive sampling approach was used to select participants in accordance with their experiences and required characteristics. A total sample of fourteen participants was selected. The data was obtained through focus group interviews, using an interview schedule (Appendix B, C, D and E) as an interview guide and the interview sessions were all recorded with an audio recorder. Separate interview schedules were used for the focus groups with the elderly participants (Appendix B – English version and D- IsiXhosa version) and the group consisting of caregivers (Appendix C – English version and E – IsiXhosa version). Thematic analysis was used to analyse and interpret results. Eight main themes were identified, namely: Living Conditions, Daily Activities, Age and Gender, Alcohol Consumption, Family Victimization, Offender Related Factors, Elderly Protection, and Intervention. These themes were identified under chapter four and discussed in the subsequent chapter. Respective sub-themes were identified under each theme and were discussed comprehensively. Key to all these themes was that most perpetrators of elderly abuse were family members, relatives and neighbours of the victim. Also worth noting is the revelation that elderly people, especially those who live in the rural areas are mostly living alone or with little children and are, therefore, without protection. , Thesis (M Soc Sc) (Criminology) -- University of Fort Hare, 2018
- Full Text:
- Date Issued: 2018
- Authors: Rasmeni, Nosabelo Lusanda
- Date: 2018
- Subjects: Older people -- Abuse of , Abused elderly -- Services for
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/19625 , vital:43153
- Description: The aim of this study was to explore factors which contribute to elder abuse in Mount Frere Town, in the Umzimvubu Local Municipality in the Eastern Cape Province. A qualitative approach was used to allow for the achievement of the research aim which was to explore contributory factors towards elderly abuse in Mount Frere Town. A purposive sampling approach was used to select participants in accordance with their experiences and required characteristics. A total sample of fourteen participants was selected. The data was obtained through focus group interviews, using an interview schedule (Appendix B, C, D and E) as an interview guide and the interview sessions were all recorded with an audio recorder. Separate interview schedules were used for the focus groups with the elderly participants (Appendix B – English version and D- IsiXhosa version) and the group consisting of caregivers (Appendix C – English version and E – IsiXhosa version). Thematic analysis was used to analyse and interpret results. Eight main themes were identified, namely: Living Conditions, Daily Activities, Age and Gender, Alcohol Consumption, Family Victimization, Offender Related Factors, Elderly Protection, and Intervention. These themes were identified under chapter four and discussed in the subsequent chapter. Respective sub-themes were identified under each theme and were discussed comprehensively. Key to all these themes was that most perpetrators of elderly abuse were family members, relatives and neighbours of the victim. Also worth noting is the revelation that elderly people, especially those who live in the rural areas are mostly living alone or with little children and are, therefore, without protection. , Thesis (M Soc Sc) (Criminology) -- University of Fort Hare, 2018
- Full Text:
- Date Issued: 2018
An investigation into births before arrival in healthcare facilities in uThungulu Health District during a period of 1 April 2014 to 31 March 2015
- Mkhwanazi, Cynthia Nqobile Nokuthula
- Authors: Mkhwanazi, Cynthia Nqobile Nokuthula
- Date: 2018
- Subjects: Childbirth Childbirth at home
- Language: English
- Type: Thesis , Masters , Public Health
- Identifier: http://hdl.handle.net/10353/11161 , vital:37198
- Description: Births Before Arrival (BBAs) in healthcare facilities is a worldwide health challenge. These births results with diverse obstetric and neonatal complications which could be avoided should delivery took place in an accredited environment. Delivery in health facility rate and Births Before Arrival is one of the vital measures used to monitor access as well as quality of Maternal and Child health services. A birth before arrival in health institutions happens accidentally and unattended by skilled health professionals outside the healthcare facilities (Lazic & Takač, 2011). It is found in both developed and developing countries with various adverse outcomes ranging from haemorrhage to death. Important strides had been taken to improve maternal and child health globally but the healthcare facilities are still experiencing an overwhelming number of births before arrival in healthcare facilities. This is a public health concern. The purpose of the study was to identify factors associated with Births before Arrival in UThungulu Health District facilities and determine the prevalence of BBAs and the neonatal and maternal morbidity and mortality associated with it. A retrospective quantitative research design was used. Maternity clinical records Reviews of 238 Births Before Arrival (BBA) in healthcare facilities that occurred between 1 April 2014 to 31 March 2015 were conducted in the six hospitals in UThungulu Health District. The births before arrival data was analysed using SPSS (Statistical Package for Social Science) version 23, for Windows and used for descriptive and inferential analysis. The reasons for BBA included poor ante natal education of mothers on early signs of labour, lack of transport, poor use of maternity waiting areas and some clinics do not conduct deliveries. The majority of study participants were single (unmarried) and this accentuates the importance of strengthening family planning coverage in this area. Poor reporting of early neonatal deaths and stillbirths was one of the significant observations made during the study. Deceased babies were left at home and buried without being registered at home affairs. Taking cognizance of diverse risks and health needs of expectant women would assist the Health department in planning for adequate coverage for provision of suitable pregnancy care that would improve the health of women and their babies.
- Full Text:
- Date Issued: 2018
- Authors: Mkhwanazi, Cynthia Nqobile Nokuthula
- Date: 2018
- Subjects: Childbirth Childbirth at home
- Language: English
- Type: Thesis , Masters , Public Health
- Identifier: http://hdl.handle.net/10353/11161 , vital:37198
- Description: Births Before Arrival (BBAs) in healthcare facilities is a worldwide health challenge. These births results with diverse obstetric and neonatal complications which could be avoided should delivery took place in an accredited environment. Delivery in health facility rate and Births Before Arrival is one of the vital measures used to monitor access as well as quality of Maternal and Child health services. A birth before arrival in health institutions happens accidentally and unattended by skilled health professionals outside the healthcare facilities (Lazic & Takač, 2011). It is found in both developed and developing countries with various adverse outcomes ranging from haemorrhage to death. Important strides had been taken to improve maternal and child health globally but the healthcare facilities are still experiencing an overwhelming number of births before arrival in healthcare facilities. This is a public health concern. The purpose of the study was to identify factors associated with Births before Arrival in UThungulu Health District facilities and determine the prevalence of BBAs and the neonatal and maternal morbidity and mortality associated with it. A retrospective quantitative research design was used. Maternity clinical records Reviews of 238 Births Before Arrival (BBA) in healthcare facilities that occurred between 1 April 2014 to 31 March 2015 were conducted in the six hospitals in UThungulu Health District. The births before arrival data was analysed using SPSS (Statistical Package for Social Science) version 23, for Windows and used for descriptive and inferential analysis. The reasons for BBA included poor ante natal education of mothers on early signs of labour, lack of transport, poor use of maternity waiting areas and some clinics do not conduct deliveries. The majority of study participants were single (unmarried) and this accentuates the importance of strengthening family planning coverage in this area. Poor reporting of early neonatal deaths and stillbirths was one of the significant observations made during the study. Deceased babies were left at home and buried without being registered at home affairs. Taking cognizance of diverse risks and health needs of expectant women would assist the Health department in planning for adequate coverage for provision of suitable pregnancy care that would improve the health of women and their babies.
- Full Text:
- Date Issued: 2018
An investigation into factors influencing lack of improvement towards achieving compliance with the quality national core standards in KSD clinics, O.R. Tambo District
- Authors: Mnyamana, Tozama Nobom
- Date: 2018
- Subjects: Health facilities--Standards , Health facilities--South Africa , Medical care--South Africa
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/19498 , vital:43133
- Description: The purpose of this study was to explore factors associated with lack of improvement in the health care provided towards achieving compliance with the National Core Standards (NCS) in King Sabata Dalindyebo (KSD) sub-district clinics, in OR Tambo District in the Eastern Cape. A quantitative design was seen as the most suitable for this study. This method was chosen as the one that would best assist the researcher to meet the objectives of the study. A purposive sampling method was chosen, according to which the researcher selected five sites; all clinics that had been previously inspected for quality standards by the Office of Health Standards Compliance at least once. Participants for the study were all employees of those clinics and one representative of the governing structure of each. Twenty-eight participants in total formed part of the study as two did not return their responses. For collecting data from the participants, self-administered questionnaires were used. Throughout the data collection process, ethical standards of nursing research regarding anonymity, confidentiality and privacy were adhered to. Collected data were analysed using the Statistical Package for the Social Sciences (SPSS) computer software. The distribution of results is presented in tables and bar graphs. The results of the study reveal that a quality-oriented culture is lacking in our clinics with poor participation of clinics teams in quality improvement processes. Leadership and management at clinic level need strengthening. It was also found that there is insufficient capacitation of departmental strategic shift innovations, revealed in the complete absence of information sharing and feedback regarding previous assessments. In addition, there is a shortage of resources including basic equipment in clinics. , Thesis (MPH) -- University of Fort Hare, 2018
- Full Text:
- Date Issued: 2018
- Authors: Mnyamana, Tozama Nobom
- Date: 2018
- Subjects: Health facilities--Standards , Health facilities--South Africa , Medical care--South Africa
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/19498 , vital:43133
- Description: The purpose of this study was to explore factors associated with lack of improvement in the health care provided towards achieving compliance with the National Core Standards (NCS) in King Sabata Dalindyebo (KSD) sub-district clinics, in OR Tambo District in the Eastern Cape. A quantitative design was seen as the most suitable for this study. This method was chosen as the one that would best assist the researcher to meet the objectives of the study. A purposive sampling method was chosen, according to which the researcher selected five sites; all clinics that had been previously inspected for quality standards by the Office of Health Standards Compliance at least once. Participants for the study were all employees of those clinics and one representative of the governing structure of each. Twenty-eight participants in total formed part of the study as two did not return their responses. For collecting data from the participants, self-administered questionnaires were used. Throughout the data collection process, ethical standards of nursing research regarding anonymity, confidentiality and privacy were adhered to. Collected data were analysed using the Statistical Package for the Social Sciences (SPSS) computer software. The distribution of results is presented in tables and bar graphs. The results of the study reveal that a quality-oriented culture is lacking in our clinics with poor participation of clinics teams in quality improvement processes. Leadership and management at clinic level need strengthening. It was also found that there is insufficient capacitation of departmental strategic shift innovations, revealed in the complete absence of information sharing and feedback regarding previous assessments. In addition, there is a shortage of resources including basic equipment in clinics. , Thesis (MPH) -- University of Fort Hare, 2018
- Full Text:
- Date Issued: 2018
An investigation into how a guided learner leadership programme can foster authentic leadership in a boys’ boarding school environment
- Authors: Cuyler, Craig
- Date: 2018
- Subjects: Educational leadership -- South Africa Boarding schools -- South Africa Boys -- South Africa
- Language: English
- Type: text , Thesis , Masters , MEd
- Identifier: http://hdl.handle.net/10962/61756 , vital:28055
- Description: This study is located within the field of Educational Leadership and Management and the research was undertaken in a boys’ private boarding school in Grahamstown, South Africa. Learner Leadership within the ELM field of study, has gained much interest in recent times and as the process of democratisation within schools continues to take place, it is important that research efforts be more focused in this area. The lack of learner voice initiatives within South African schools, in spite of policies being in place that encourage it, has created the impression that learner leadership is far more about rhetoric than actual practice. This appears to be the case in private education as well, owing to practices that are reliant on hierarchy and tradition to cement their position within these schools. It was with this in mind that a formative peer mentoring intervention was put in place in a boarding house at St Andrew’s College, a private boys’ school in Grahamstown, South Africa, with the object of developing authentic leadership in a boarding house context. This study was framed by Cultural Historical Activity Theory and sought to investigate how a guided learner leadership programme could foster authentic leadership in a boys’ boarding school context. The intervention consisted of three phases: 1) a pre-intervention questionnaire; 2) a Mentoring Course, during which Grade 12 learners were trained how to be mentors; and 3) a Mentoring Programme, during which Grade 12 learners were each allocated a Grade 8 learner to mentor during the course of the year. Data was collected during all three phases of the intervention and said data was obtained via questionnaires, interviews and from notes kept in an observation journal. The data was analysed inductively and later by using Cultural Historical Activity Theory, which acted as a lens through which data was interpreted. The findings reflected that learners responded well to the Mentoring Course and that they participated as active agents of change. It was during the Mentoring Programme, where contradictions became apparent and where the default to practices associated with hierarchy and tradition became evident. The Mentoring Programme did reflect some positive results, such as learners taking more ownership of the Programme and becoming critical of their own practice as mentors. This led to the further take-up of the Mentoring Programme in other boarding houses at St Andrew’s College after the intervention, and the course continues to grow and improve. My recommendations include that broader research be undertaken generally, to understand the role that tradition and hierarchy play, particularly in private schools, so that more authentic learner leadership can be put in place, and to conduct a longitudinal study to establish the success of the Mentoring Programme at St Andrew’s College specifically, over time.
- Full Text:
- Date Issued: 2018
- Authors: Cuyler, Craig
- Date: 2018
- Subjects: Educational leadership -- South Africa Boarding schools -- South Africa Boys -- South Africa
- Language: English
- Type: text , Thesis , Masters , MEd
- Identifier: http://hdl.handle.net/10962/61756 , vital:28055
- Description: This study is located within the field of Educational Leadership and Management and the research was undertaken in a boys’ private boarding school in Grahamstown, South Africa. Learner Leadership within the ELM field of study, has gained much interest in recent times and as the process of democratisation within schools continues to take place, it is important that research efforts be more focused in this area. The lack of learner voice initiatives within South African schools, in spite of policies being in place that encourage it, has created the impression that learner leadership is far more about rhetoric than actual practice. This appears to be the case in private education as well, owing to practices that are reliant on hierarchy and tradition to cement their position within these schools. It was with this in mind that a formative peer mentoring intervention was put in place in a boarding house at St Andrew’s College, a private boys’ school in Grahamstown, South Africa, with the object of developing authentic leadership in a boarding house context. This study was framed by Cultural Historical Activity Theory and sought to investigate how a guided learner leadership programme could foster authentic leadership in a boys’ boarding school context. The intervention consisted of three phases: 1) a pre-intervention questionnaire; 2) a Mentoring Course, during which Grade 12 learners were trained how to be mentors; and 3) a Mentoring Programme, during which Grade 12 learners were each allocated a Grade 8 learner to mentor during the course of the year. Data was collected during all three phases of the intervention and said data was obtained via questionnaires, interviews and from notes kept in an observation journal. The data was analysed inductively and later by using Cultural Historical Activity Theory, which acted as a lens through which data was interpreted. The findings reflected that learners responded well to the Mentoring Course and that they participated as active agents of change. It was during the Mentoring Programme, where contradictions became apparent and where the default to practices associated with hierarchy and tradition became evident. The Mentoring Programme did reflect some positive results, such as learners taking more ownership of the Programme and becoming critical of their own practice as mentors. This led to the further take-up of the Mentoring Programme in other boarding houses at St Andrew’s College after the intervention, and the course continues to grow and improve. My recommendations include that broader research be undertaken generally, to understand the role that tradition and hierarchy play, particularly in private schools, so that more authentic learner leadership can be put in place, and to conduct a longitudinal study to establish the success of the Mentoring Programme at St Andrew’s College specifically, over time.
- Full Text:
- Date Issued: 2018
An investigation into teaching mathematics using a visualisation approach to recontextualise indigenous knowledge
- Authors: Tshithigona, Gaus
- Date: 2018
- Subjects: Ethnoscience Namibia , Mathematics Study and teaching (Elementary) Namibia , Visual learning , Visualization , Interdisciplinary approach to knowledge , Teacher effectiveness Namibia , Traditional ecological knowledge Namibia
- Language: English
- Type: text , Thesis , Masters , MEd
- Identifier: http://hdl.handle.net/10962/62461 , vital:28195
- Description: It can be argued that the Namibian curriculum is largely influenced by a Western epistemology. However, many studies suggest that learners make powerful meanings of mathematical concepts they are learning if they visualise these and experience them in relation to what they already know from their own cultural backgrounds. According to the Namibian National Curriculum for Basic Education (NNCBE, 2010), it is not only important for learners to acquire mathematical knowledge and skills, but also to develop and grow their identities, cultures and values as individuals. The aim of this study was to explore and investigate how selected mathematics teachers employ visualisation as a teaching approach to re-contextualise indigenous knowledge (IK). The study set out to explore how conceptual understanding is enhanced by participation in an intervention programme. The use of visualisation is considered an important mediating and pedagogical tool in the mathematics classroom to enhance the mathematics learning of learners. The research is informed by a socio-cultural theory of learning and is located within an interpretive paradigm. The study was conducted at four schools in the Oshana region of Namibia and involved four mathematics teachers who were purposefully selected due to their willingness to use visualisation-IK approaches in their teaching, based on their responses to the survey. The methodologies used are qualitative and quantitative case study. To gather data, document analysis, a survey, lesson observations and focus group interviews were used. The study found that most teachers in the Oshana region have an understanding of the effective use of visualisation-IK approaches. However, the study revealed that visualisation- IK approaches are mostly used in grades 4 to 7 mathematics classrooms. It also discovered that most of the visualisation approaches that teachers employed aligned well with the curriculum and promoted conceptual understanding in the teaching of mathematics. The results of the study showed that teachers have experience of the enabling and constraining factors in using a visualisation-IK approach to teaching mathematics.
- Full Text:
- Date Issued: 2018
- Authors: Tshithigona, Gaus
- Date: 2018
- Subjects: Ethnoscience Namibia , Mathematics Study and teaching (Elementary) Namibia , Visual learning , Visualization , Interdisciplinary approach to knowledge , Teacher effectiveness Namibia , Traditional ecological knowledge Namibia
- Language: English
- Type: text , Thesis , Masters , MEd
- Identifier: http://hdl.handle.net/10962/62461 , vital:28195
- Description: It can be argued that the Namibian curriculum is largely influenced by a Western epistemology. However, many studies suggest that learners make powerful meanings of mathematical concepts they are learning if they visualise these and experience them in relation to what they already know from their own cultural backgrounds. According to the Namibian National Curriculum for Basic Education (NNCBE, 2010), it is not only important for learners to acquire mathematical knowledge and skills, but also to develop and grow their identities, cultures and values as individuals. The aim of this study was to explore and investigate how selected mathematics teachers employ visualisation as a teaching approach to re-contextualise indigenous knowledge (IK). The study set out to explore how conceptual understanding is enhanced by participation in an intervention programme. The use of visualisation is considered an important mediating and pedagogical tool in the mathematics classroom to enhance the mathematics learning of learners. The research is informed by a socio-cultural theory of learning and is located within an interpretive paradigm. The study was conducted at four schools in the Oshana region of Namibia and involved four mathematics teachers who were purposefully selected due to their willingness to use visualisation-IK approaches in their teaching, based on their responses to the survey. The methodologies used are qualitative and quantitative case study. To gather data, document analysis, a survey, lesson observations and focus group interviews were used. The study found that most teachers in the Oshana region have an understanding of the effective use of visualisation-IK approaches. However, the study revealed that visualisation- IK approaches are mostly used in grades 4 to 7 mathematics classrooms. It also discovered that most of the visualisation approaches that teachers employed aligned well with the curriculum and promoted conceptual understanding in the teaching of mathematics. The results of the study showed that teachers have experience of the enabling and constraining factors in using a visualisation-IK approach to teaching mathematics.
- Full Text:
- Date Issued: 2018
An investigation into the bacterial biosynthetic origins of bioactive natural products isolated from South African latrunculid sponges
- Authors: Waterworth, Samantha Che
- Date: 2018
- Subjects: Marine biodiversity , Metagenomics , Sponges Biotechnology , Spirochetes , Natural products Biotechnology
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/61826 , vital:28065
- Description: Several pyrroloiminoquinone alkaloids exhibiting cytotoxic, anti-tumour activity have been isolated from sponges within the Latrunculiidae family that are endemic to the South African coastline. Other, structurally similar pyrroloiminoquinone compounds have been isolated from geographically distant and phylogenetically distinct marine sponges, as well as terrestrial myxomycetes which suggested that sponge-associated bacteria may be the true biosynthetic origin of pyrroloiminoquinone compounds. Previous studies have shown that there is conservation of spirochete and betaproteobacterial species in the bacterial communities associated with South African Latrunculiidae sponges and it was proposed that these conserved bacteria represented candidate pyrroloiminoquinone-producers. This study aimed to confirm the conserved dominance of betaproteobacteria and spirochetes within bacterial communities associated with South African latrunculid sponges and employed a shotgun metagenomic approach to assess the functional and biosynthetic potential of associated microbiota in Tsitsikamma favus sponges. Clustering of assembled contigs revealed twenty-three putative bacterial genomes, of which, two were identified as representatives of the conserved betaproteobacteria and spirochete species previously identified in Tsitsikamma sponges. It was shown that the spirochete was most likely an obligate symbiont that benefitted the host sponge through possible defence against pathogenic bacteria and/or nutrient acquisition. The putative genome representing the conserved betaproteobacteria was found to be heavily contaminated and further sequencing is required to accurately resolve the genome for functional characterization. Several biosynthetic gene clusters were identified and demonstrated the bioactive potential of Tsitsikamma favus-associated bacteria. A biosynthetic gene cluster was identified on an unclustered contig that included several genetic features that were indicative of possible pyrroloiminoquinone production.
- Full Text:
- Date Issued: 2018
- Authors: Waterworth, Samantha Che
- Date: 2018
- Subjects: Marine biodiversity , Metagenomics , Sponges Biotechnology , Spirochetes , Natural products Biotechnology
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/61826 , vital:28065
- Description: Several pyrroloiminoquinone alkaloids exhibiting cytotoxic, anti-tumour activity have been isolated from sponges within the Latrunculiidae family that are endemic to the South African coastline. Other, structurally similar pyrroloiminoquinone compounds have been isolated from geographically distant and phylogenetically distinct marine sponges, as well as terrestrial myxomycetes which suggested that sponge-associated bacteria may be the true biosynthetic origin of pyrroloiminoquinone compounds. Previous studies have shown that there is conservation of spirochete and betaproteobacterial species in the bacterial communities associated with South African Latrunculiidae sponges and it was proposed that these conserved bacteria represented candidate pyrroloiminoquinone-producers. This study aimed to confirm the conserved dominance of betaproteobacteria and spirochetes within bacterial communities associated with South African latrunculid sponges and employed a shotgun metagenomic approach to assess the functional and biosynthetic potential of associated microbiota in Tsitsikamma favus sponges. Clustering of assembled contigs revealed twenty-three putative bacterial genomes, of which, two were identified as representatives of the conserved betaproteobacteria and spirochete species previously identified in Tsitsikamma sponges. It was shown that the spirochete was most likely an obligate symbiont that benefitted the host sponge through possible defence against pathogenic bacteria and/or nutrient acquisition. The putative genome representing the conserved betaproteobacteria was found to be heavily contaminated and further sequencing is required to accurately resolve the genome for functional characterization. Several biosynthetic gene clusters were identified and demonstrated the bioactive potential of Tsitsikamma favus-associated bacteria. A biosynthetic gene cluster was identified on an unclustered contig that included several genetic features that were indicative of possible pyrroloiminoquinone production.
- Full Text:
- Date Issued: 2018
An investigation into the bacterial communities associated with pyrroloiminoquinone-producing South African latrunculid sponges
- Authors: Hilliar, Storm Hannah
- Date: 2018
- Subjects: Sponges South Africa Algoa Bay , Betaproteobacteria , Spirochaeta , Symbiosis , Bacterial communities
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/62112 , vital:28128
- Description: Marine sponges belonging to the family Latrunculiidae are known for their production of cytotoxic pyrroloiminoquinone alkaloids and the South African coast provides a unique environment for the exploitation of these potent bioactive compounds. The isolation of structurally similar pyrroloiminoquinone compounds from unrelated, non poriferan sources has led to the suggestion that South African latrunculid pyrroloiminoquinones may be secondary metabolites produced by sponge associated microbial symbionts. Previous studies investigating the bacterial communities of South African latrunculid sponges have shown the conservation of distinct microbial populations with unusual bacterial taxa dominated by a novel betaproteobacterial and spirochete species. This study describes the further investigation into these associated bacterial communities, their conservation and sponge microbiome comparisons across spatial, temporal and environmental scales. The bacterial communities associated with seven latrunculid species representing three genera (Tsitsikamma, Cyclacanthia and Latrunculia) were characterized as well as a Mycale and Tethya rubra species. Latrunculid sponge microbiomes were significantly different from those associated with sympatric outlier sponge species and the surrounding environment. The bacterial communities associated with latrunculid sponges appear host specific with the conservation of two dominant bacterial symbionts which mirror the phylogeny of their host species. , Thesis (MSc) -- Faculty of Science, Biochemistry and Microbiology, 2018
- Full Text:
- Date Issued: 2018
- Authors: Hilliar, Storm Hannah
- Date: 2018
- Subjects: Sponges South Africa Algoa Bay , Betaproteobacteria , Spirochaeta , Symbiosis , Bacterial communities
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/62112 , vital:28128
- Description: Marine sponges belonging to the family Latrunculiidae are known for their production of cytotoxic pyrroloiminoquinone alkaloids and the South African coast provides a unique environment for the exploitation of these potent bioactive compounds. The isolation of structurally similar pyrroloiminoquinone compounds from unrelated, non poriferan sources has led to the suggestion that South African latrunculid pyrroloiminoquinones may be secondary metabolites produced by sponge associated microbial symbionts. Previous studies investigating the bacterial communities of South African latrunculid sponges have shown the conservation of distinct microbial populations with unusual bacterial taxa dominated by a novel betaproteobacterial and spirochete species. This study describes the further investigation into these associated bacterial communities, their conservation and sponge microbiome comparisons across spatial, temporal and environmental scales. The bacterial communities associated with seven latrunculid species representing three genera (Tsitsikamma, Cyclacanthia and Latrunculia) were characterized as well as a Mycale and Tethya rubra species. Latrunculid sponge microbiomes were significantly different from those associated with sympatric outlier sponge species and the surrounding environment. The bacterial communities associated with latrunculid sponges appear host specific with the conservation of two dominant bacterial symbionts which mirror the phylogeny of their host species. , Thesis (MSc) -- Faculty of Science, Biochemistry and Microbiology, 2018
- Full Text:
- Date Issued: 2018
An investigation into the effectiveness of financial management systems in the public service: a case study of the department of health, Eastern Cape Province
- Authors: Galada, Unathi Luthando
- Date: 2018
- Subjects: Financial management -- South Africa -- Eastern Cape , Finance, Public -- South Africa -- Eastern Cape Performance -- Measurement -- Evaluation
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: http://hdl.handle.net/10948/30227 , vital:30904
- Description: The aim of this study is to investigate the effective and efficient of the financial management systems and its translation into good governance in the Department of Health in order to meet all the compliance requirements as contained in legislations. Financial management is a critical element for good governance. It requires a great deal of attention with parallel internal systems and control that support it for optimal performance. To that, the Public Finance Management Act, 1999 (Act 1 of 1999) and National Treasury Regulations require all government departments to ensure accountability on resource allocated yet able to measure value for money in relation to the delivery of goods and services. The Eastern Cape is one of five provinces with at least 60% of its population living in rural parts serviced by small towns. There is a total of 72 small towns in the province. The province has a legacy of endemic rural poverty and is characterized by the historical neglect of rural areas. The Eastern Cape still has many people moving to other provinces and to the two main cities of East London and Port Elizabeth, in search of employment. (ECSECC, 2006). The compliance to all key financial management legislation and policies has been a constant challenge faced by the Department of Health and the province, which has been largely caused by high levels of corruption, poor financial management systems, poor management tools, poor internal resource controls and maladministration practices (Auditor General Report, 2013/14). As a result, several incidents of dissatisfaction by members of the communities has been on the public domain, calling for government to deliver on quality health services throughout the province. The Public Finance Management Act, Act No. 1 of 1999, with regards to government’s utilisation of financial resources outlines the following objectives: Modernise the system of financial management in the public sector; Enable public sector managers to manage, but at the same time be held more accountable; Ensure the timely provision of quality information; and Eliminate the waste and corruption in the use of public assets. The Auditor General, as the supreme audit institution of the Republic, must, in terms of section 4(1) of the Public Audit Act (Act No. 25 of 2004), audit and report on the accounts, financial statements and financial statement of all government entities. Based on the 2014/15 Consolidated General Report issued by the Auditor General, various government entities were found not to have complied with the legislation relating to the payment of suppliers. The Constitution Republic of South Africa (Act 108 of 1996) makes a provision that all the three government spheres to be developmental-oriented in nature with clear accountability and transparency lines towards service delivery. On the same note , The Public Finance Management Act of 1999 (PFMA) is meant “To regulate financial management in the national government and provincial governments; to ensure that all revenue, expenditure, assets and liabilities of those governments are managed efficiently and effectively; to provide for the responsibilities of persons entrusted with financial management in those governments; and to provide for matters connected therewith”. Based on these legislations, the department of health is not isolated from the responsibility as it forms part of the provincial government, which is the provincial sphere. With the above introductory contentions from different authors and researchers, this study will provide more detail on the policy documents namely; PFMA so to establish the nature and extent to which effective and efficient Financial Management System has improved good governance in the Department of Health, Eastern Cape Province.
- Full Text:
- Date Issued: 2018
- Authors: Galada, Unathi Luthando
- Date: 2018
- Subjects: Financial management -- South Africa -- Eastern Cape , Finance, Public -- South Africa -- Eastern Cape Performance -- Measurement -- Evaluation
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: http://hdl.handle.net/10948/30227 , vital:30904
- Description: The aim of this study is to investigate the effective and efficient of the financial management systems and its translation into good governance in the Department of Health in order to meet all the compliance requirements as contained in legislations. Financial management is a critical element for good governance. It requires a great deal of attention with parallel internal systems and control that support it for optimal performance. To that, the Public Finance Management Act, 1999 (Act 1 of 1999) and National Treasury Regulations require all government departments to ensure accountability on resource allocated yet able to measure value for money in relation to the delivery of goods and services. The Eastern Cape is one of five provinces with at least 60% of its population living in rural parts serviced by small towns. There is a total of 72 small towns in the province. The province has a legacy of endemic rural poverty and is characterized by the historical neglect of rural areas. The Eastern Cape still has many people moving to other provinces and to the two main cities of East London and Port Elizabeth, in search of employment. (ECSECC, 2006). The compliance to all key financial management legislation and policies has been a constant challenge faced by the Department of Health and the province, which has been largely caused by high levels of corruption, poor financial management systems, poor management tools, poor internal resource controls and maladministration practices (Auditor General Report, 2013/14). As a result, several incidents of dissatisfaction by members of the communities has been on the public domain, calling for government to deliver on quality health services throughout the province. The Public Finance Management Act, Act No. 1 of 1999, with regards to government’s utilisation of financial resources outlines the following objectives: Modernise the system of financial management in the public sector; Enable public sector managers to manage, but at the same time be held more accountable; Ensure the timely provision of quality information; and Eliminate the waste and corruption in the use of public assets. The Auditor General, as the supreme audit institution of the Republic, must, in terms of section 4(1) of the Public Audit Act (Act No. 25 of 2004), audit and report on the accounts, financial statements and financial statement of all government entities. Based on the 2014/15 Consolidated General Report issued by the Auditor General, various government entities were found not to have complied with the legislation relating to the payment of suppliers. The Constitution Republic of South Africa (Act 108 of 1996) makes a provision that all the three government spheres to be developmental-oriented in nature with clear accountability and transparency lines towards service delivery. On the same note , The Public Finance Management Act of 1999 (PFMA) is meant “To regulate financial management in the national government and provincial governments; to ensure that all revenue, expenditure, assets and liabilities of those governments are managed efficiently and effectively; to provide for the responsibilities of persons entrusted with financial management in those governments; and to provide for matters connected therewith”. Based on these legislations, the department of health is not isolated from the responsibility as it forms part of the provincial government, which is the provincial sphere. With the above introductory contentions from different authors and researchers, this study will provide more detail on the policy documents namely; PFMA so to establish the nature and extent to which effective and efficient Financial Management System has improved good governance in the Department of Health, Eastern Cape Province.
- Full Text:
- Date Issued: 2018
An investigation into the introduction of a new wealth tax in South Africa
- Authors: Arendse, Jacqueline A
- Date: 2018
- Subjects: Wealth tax -- Law and legislation -- South Africa , Taxation -- Law and legislation -- South Africa , Income tax -- South Africa , Fiscal policy -- South Africa , South Africa -- Economic conditions , Income distribution -- South Africa
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/61379 , vital:28020
- Description: In a world of economic uncertainty and manifold social problems, South Africa has its own unique challenges of low economic growth, persistent budget deficits that produce increasing government debt and the highest level of economic inequality in the world. The history of injustice and economic marginalisation and the failure of the economy to provide inclusive growth drives an urgent need to address economic inequality through tax policy, placing ever more focus on wealth taxes as a possible solution. There is a hope is that taxing the wealthy may provide the opportunity to redistribute desperately-needed resources to those denied the opportunity to build wealth and who are trapped in the cycle of poverty. Yet, as appealing as a new wealth tax may seem, the introduction of such a tax carries with it a range of risks, not all of which are known. Of great concern is the possible effect on the economy, which, in its vulnerable state, cannot afford any loss of capital and investment. Very little research has been done on wealth tax in the South African context and there is a dearth of literature focusing on the views and perceptions of the wealthy individuals themselves. This qualitative study investigates the merits and disadvantages of a new wealth tax and seeks to identify any unintended consequences that could result from the implementation of a new wealth tax in South Africa, drawing from historical and international experience and primary data obtained from interviews with individuals likely to be affected by such a tax. Having explored the literature and international experiences with wealth tax and having probed the thinking of wealthy individuals who would be the payers of a wealth tax, the study finds that a new wealth tax may contribute towards the progressivity of the tax system, but it is doubtful whether such a tax would provide a sustainable revenue stream that would be sufficient to address economic inequality and there is a risk of causing harm to the economy. Recognising that the motivation for wealth taxes is often driven more by political argument and public perception than by rational quantitative analysis, the study also anticipates the introduction of a new wealth tax and suggests guidelines for the design of such a tax within the framework for evaluating a good tax system. This study informs the debate on wealth taxes in South Africa and contributes to the design of such a tax, should it be implemented.
- Full Text:
- Date Issued: 2018
- Authors: Arendse, Jacqueline A
- Date: 2018
- Subjects: Wealth tax -- Law and legislation -- South Africa , Taxation -- Law and legislation -- South Africa , Income tax -- South Africa , Fiscal policy -- South Africa , South Africa -- Economic conditions , Income distribution -- South Africa
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/61379 , vital:28020
- Description: In a world of economic uncertainty and manifold social problems, South Africa has its own unique challenges of low economic growth, persistent budget deficits that produce increasing government debt and the highest level of economic inequality in the world. The history of injustice and economic marginalisation and the failure of the economy to provide inclusive growth drives an urgent need to address economic inequality through tax policy, placing ever more focus on wealth taxes as a possible solution. There is a hope is that taxing the wealthy may provide the opportunity to redistribute desperately-needed resources to those denied the opportunity to build wealth and who are trapped in the cycle of poverty. Yet, as appealing as a new wealth tax may seem, the introduction of such a tax carries with it a range of risks, not all of which are known. Of great concern is the possible effect on the economy, which, in its vulnerable state, cannot afford any loss of capital and investment. Very little research has been done on wealth tax in the South African context and there is a dearth of literature focusing on the views and perceptions of the wealthy individuals themselves. This qualitative study investigates the merits and disadvantages of a new wealth tax and seeks to identify any unintended consequences that could result from the implementation of a new wealth tax in South Africa, drawing from historical and international experience and primary data obtained from interviews with individuals likely to be affected by such a tax. Having explored the literature and international experiences with wealth tax and having probed the thinking of wealthy individuals who would be the payers of a wealth tax, the study finds that a new wealth tax may contribute towards the progressivity of the tax system, but it is doubtful whether such a tax would provide a sustainable revenue stream that would be sufficient to address economic inequality and there is a risk of causing harm to the economy. Recognising that the motivation for wealth taxes is often driven more by political argument and public perception than by rational quantitative analysis, the study also anticipates the introduction of a new wealth tax and suggests guidelines for the design of such a tax within the framework for evaluating a good tax system. This study informs the debate on wealth taxes in South Africa and contributes to the design of such a tax, should it be implemented.
- Full Text:
- Date Issued: 2018
An investigation into the role of ethics and morality in translation with particular reference to English and isiXhosa
- Authors: Ntantiso, Nobuntu Faith
- Date: 2018
- Subjects: Translating and interpreting -- Research , Language -- Moral and ethical aspects English language -- Moral and ethical aspects Xhosa language -- Moral and ethical aspects
- Language: English
- Type: Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10948/33780 , vital:33028
- Description: Translation is one of the oldest professions in the world. It is also a unique profession because most of the time translation professionals work in isolation. They work in their private spaces without any supervision. This means that the decision on how to translate lies solely on the translator, and on his or her individual ethical and moral integrity. As a result, translators have a responsibility to produce translations that are ethically and morally acceptable. This study will consequently investigate the role of ethics and morality in translation with particular reference to English and isiXhosa. This study will discuss how ethics and morality play a role in translation. It will first state the objectives, define the relevant terminology and provide a list of abbreviations that will be used in the study. It will also explain the significance of the study, analyse and state the problem, do a literature review and indicate the methodology that it will use. In addition, it will mention how the chapters have been structured and the topics that will be covered in each chapter.
- Full Text:
- Date Issued: 2018
- Authors: Ntantiso, Nobuntu Faith
- Date: 2018
- Subjects: Translating and interpreting -- Research , Language -- Moral and ethical aspects English language -- Moral and ethical aspects Xhosa language -- Moral and ethical aspects
- Language: English
- Type: Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10948/33780 , vital:33028
- Description: Translation is one of the oldest professions in the world. It is also a unique profession because most of the time translation professionals work in isolation. They work in their private spaces without any supervision. This means that the decision on how to translate lies solely on the translator, and on his or her individual ethical and moral integrity. As a result, translators have a responsibility to produce translations that are ethically and morally acceptable. This study will consequently investigate the role of ethics and morality in translation with particular reference to English and isiXhosa. This study will discuss how ethics and morality play a role in translation. It will first state the objectives, define the relevant terminology and provide a list of abbreviations that will be used in the study. It will also explain the significance of the study, analyse and state the problem, do a literature review and indicate the methodology that it will use. In addition, it will mention how the chapters have been structured and the topics that will be covered in each chapter.
- Full Text:
- Date Issued: 2018
An investigation into the translation of Isixhosa kinship lexical items into English
- Authors: Majikija, Sandisiwe
- Date: 2018
- Subjects: Sociolinguistics , Communication -- Language Xhosa (African people) -- Kinship -- Language Lexicology -- Kinship
- Language: English
- Type: Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10948/22607 , vital:30024
- Description: This study investigates the problem of non- equivalence in the translation of IsiXhosa kinship lexical items or concepts into English. Venuti (2012:5) says translation can be seen as ‘a set of changing relationships between the relative autonomy of the translated text…and…equivalence and function.’ Equivalence, of which non- equivalence is the antithesis, includes “accuracy,’ adequacy,’ ‘correctness,’ ‘correspondence,’ or ‘identity’. A variable notion, it indicates how translation is connected to the source text. A break in that connection results in non-equivalence. Therein lies the problem. The current study examines these notions, among other things, as they apply to the translation of isiXhosa kinship lexical items into English. As Venuti (2010) points out translation rests on particular assumptions about language use. These assumptions draw on two particular, emerging theories or approaches namely, the ‘instrumental and the hermeneutic’ as will be discussed. Isicatshulwa Olu phando lumalunga nengxaki yokungafani kwentsingiselo yamagama okanye ingqikelelo xa kuguqulelwa esiNgesini amagama okuzalana esiXhoseni. UVenuti (2012:5) uthi inguqulo ingajongwa njengokujika konxulumano oluguqukayo phakathi kokungaxhomekeki kwesiqendwana esiguqulwayo nonxulumano. Unxulumano oluphikisana nokunganxulumani, lubandakanya ‘ukuchaneka,’ ‘ukufanela,’ ‘ukulunga,’ ukungqinelana,’ okanye ‘ukufana twatse.’ Le ngcingane iguquguqukayo ibonisa indlela inguqulo ihambelana ngayo nesiqendwana esiguqulwayo. Xa oko kuhambelana kuthe kwangabikho, loo nto izala ukunganxulumani. Ilapho ke ingxaki. Olu phando luphonononga ezi ngcingane, phakathi kwezinye zezinto, njengoko zisebenza kwinguqulelo esiNgesini kwamagama okuzalana esiXhoseni. Njengoko uVenuti (2010) abonisayo, inguqulo ingqiyame ngeengcinga ezithile ezimbini malunga nokusetyenziswa kolwimi. Ezi ngcinga ziphenjelelwa ziinkcazo eziziingcingane ezithile zamva nje. Zibizwa ‘i-instrumental,’ ‘ne- hermeneutic,’ njengoko uphando olu luza kuxoxa ngazo.
- Full Text:
- Date Issued: 2018
- Authors: Majikija, Sandisiwe
- Date: 2018
- Subjects: Sociolinguistics , Communication -- Language Xhosa (African people) -- Kinship -- Language Lexicology -- Kinship
- Language: English
- Type: Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10948/22607 , vital:30024
- Description: This study investigates the problem of non- equivalence in the translation of IsiXhosa kinship lexical items or concepts into English. Venuti (2012:5) says translation can be seen as ‘a set of changing relationships between the relative autonomy of the translated text…and…equivalence and function.’ Equivalence, of which non- equivalence is the antithesis, includes “accuracy,’ adequacy,’ ‘correctness,’ ‘correspondence,’ or ‘identity’. A variable notion, it indicates how translation is connected to the source text. A break in that connection results in non-equivalence. Therein lies the problem. The current study examines these notions, among other things, as they apply to the translation of isiXhosa kinship lexical items into English. As Venuti (2010) points out translation rests on particular assumptions about language use. These assumptions draw on two particular, emerging theories or approaches namely, the ‘instrumental and the hermeneutic’ as will be discussed. Isicatshulwa Olu phando lumalunga nengxaki yokungafani kwentsingiselo yamagama okanye ingqikelelo xa kuguqulelwa esiNgesini amagama okuzalana esiXhoseni. UVenuti (2012:5) uthi inguqulo ingajongwa njengokujika konxulumano oluguqukayo phakathi kokungaxhomekeki kwesiqendwana esiguqulwayo nonxulumano. Unxulumano oluphikisana nokunganxulumani, lubandakanya ‘ukuchaneka,’ ‘ukufanela,’ ‘ukulunga,’ ukungqinelana,’ okanye ‘ukufana twatse.’ Le ngcingane iguquguqukayo ibonisa indlela inguqulo ihambelana ngayo nesiqendwana esiguqulwayo. Xa oko kuhambelana kuthe kwangabikho, loo nto izala ukunganxulumani. Ilapho ke ingxaki. Olu phando luphonononga ezi ngcingane, phakathi kwezinye zezinto, njengoko zisebenza kwinguqulelo esiNgesini kwamagama okuzalana esiXhoseni. Njengoko uVenuti (2010) abonisayo, inguqulo ingqiyame ngeengcinga ezithile ezimbini malunga nokusetyenziswa kolwimi. Ezi ngcinga ziphenjelelwa ziinkcazo eziziingcingane ezithile zamva nje. Zibizwa ‘i-instrumental,’ ‘ne- hermeneutic,’ njengoko uphando olu luza kuxoxa ngazo.
- Full Text:
- Date Issued: 2018
An investigation of mitochondrial dynamics and networks observed within human undifferentiated and differentiated cell lines
- Authors: Houseman, Pascalené Shannon
- Date: 2018
- Subjects: Mitochondria , Mitochondrial pathology , Degeneration (Pathology) , Mesenchymal stem cells , Neural stem cells , Cell lines , Reactive oxygen species (ROS)
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/60687 , vital:27816
- Description: Mitochondrial dynamics refers to a series of constant division and fusion cycles that form interconnecting networks within healthy cells. Reactive oxygen species (ROS) are the byproducts of cellular redox reactions, and, when in excess, have been linked to degenerative diseases and aging. Mesenchymal stem cells (MSCs) require a niche that presents with low levels of ROS; this enables the stem cell to maintain its “sternness”, the stem cell population, as well as the ability to adhere, migrate, and proliferate. If ROS levels increase within the MSC niche, inhibition of cellular adhesion and migration occurs. In contrast, neural stem cells require a niche that presents with a high level of ROS, aiding in their proliferative, self- renewing capacities. Investigations into what constitutes a healthy mitochondrial network versus the disease state of the network are required in order to determine what promotes degeneration and aging within stem cells. It was hypothesized that increased levels of ROS would stunt the ability of MSCs to attach and migrate, and hinder their abilities of proliferation and differentiation. In contrast, neuronal differentiation would present with an increased proliferation. This led to the investigation into the effects of ROS and oxidative stress, and the resulting mitochondrial dynamics, have on undifferentiated and differentiated mesenchymal stem and SH-SY5Y cells. Upon the addition of non-lethal S3I-201 (STAT3 has been linked to a reduction in ROS) to MSCs, an increase in ROS was observed. Higher concentrations of STAT3 inhibitor resulted in a decrease in MSC attachment and proliferation. When exposed to similar conditions, the SH-SY5Y cells underwent an increased proliferation; due to multiple restrictions, they were not used any further within the study. Mitochondrial dynamics were observed using a fusion promoter (M1) and a fission inhibitor (Mdivi-1); the MSCs were dosed with varying concentrations in order to determine the effects that mitochondrial dysfunction may have on the established networks, and cell survival. The mitochondria within MSCs migrated to the extensions of the cell, and displayed an alteration in morphology, or were clustered around the nucleus and/or the lipid deposits. These high density clusters correlated with a high intensity of fluorescence using 2’,7’- dichlorofluorescein diacetate. In conclusion, varying concentrations of ROS have different effects on MSCs in terms of overall maintenance and function; mitochondrial dynamics play an important role in cell survivability and the fate of stem cell differentiation. Further investigation into the mitochondrial dynamics and networks of these cell lines and their differentiated progeny is required.
- Full Text:
- Date Issued: 2018
- Authors: Houseman, Pascalené Shannon
- Date: 2018
- Subjects: Mitochondria , Mitochondrial pathology , Degeneration (Pathology) , Mesenchymal stem cells , Neural stem cells , Cell lines , Reactive oxygen species (ROS)
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/60687 , vital:27816
- Description: Mitochondrial dynamics refers to a series of constant division and fusion cycles that form interconnecting networks within healthy cells. Reactive oxygen species (ROS) are the byproducts of cellular redox reactions, and, when in excess, have been linked to degenerative diseases and aging. Mesenchymal stem cells (MSCs) require a niche that presents with low levels of ROS; this enables the stem cell to maintain its “sternness”, the stem cell population, as well as the ability to adhere, migrate, and proliferate. If ROS levels increase within the MSC niche, inhibition of cellular adhesion and migration occurs. In contrast, neural stem cells require a niche that presents with a high level of ROS, aiding in their proliferative, self- renewing capacities. Investigations into what constitutes a healthy mitochondrial network versus the disease state of the network are required in order to determine what promotes degeneration and aging within stem cells. It was hypothesized that increased levels of ROS would stunt the ability of MSCs to attach and migrate, and hinder their abilities of proliferation and differentiation. In contrast, neuronal differentiation would present with an increased proliferation. This led to the investigation into the effects of ROS and oxidative stress, and the resulting mitochondrial dynamics, have on undifferentiated and differentiated mesenchymal stem and SH-SY5Y cells. Upon the addition of non-lethal S3I-201 (STAT3 has been linked to a reduction in ROS) to MSCs, an increase in ROS was observed. Higher concentrations of STAT3 inhibitor resulted in a decrease in MSC attachment and proliferation. When exposed to similar conditions, the SH-SY5Y cells underwent an increased proliferation; due to multiple restrictions, they were not used any further within the study. Mitochondrial dynamics were observed using a fusion promoter (M1) and a fission inhibitor (Mdivi-1); the MSCs were dosed with varying concentrations in order to determine the effects that mitochondrial dysfunction may have on the established networks, and cell survival. The mitochondria within MSCs migrated to the extensions of the cell, and displayed an alteration in morphology, or were clustered around the nucleus and/or the lipid deposits. These high density clusters correlated with a high intensity of fluorescence using 2’,7’- dichlorofluorescein diacetate. In conclusion, varying concentrations of ROS have different effects on MSCs in terms of overall maintenance and function; mitochondrial dynamics play an important role in cell survivability and the fate of stem cell differentiation. Further investigation into the mitochondrial dynamics and networks of these cell lines and their differentiated progeny is required.
- Full Text:
- Date Issued: 2018