Adsorption of antibiotic-resistant bacteria and their cell-free deoxyribonucleic acid harbouring resistance genes in drinking water with metal oxides
- Authors: Tobechukwu, Anthony Eric
- Date: 2022
- Subjects: Metallic oxides , DNA -- Synthesis , Drinking water
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/27787 , vital:69494
- Description: Access to cleaner water is essential to human health. The incidence of Antibiotic Resistant Bacteria ARB in drinking water and subsequent proliferation of Antibiotic Resistance Genes ARGs in drinking water is a concern for public health. The free DNA cassette harboring antibiotic resistance gene in drinking water has been linked with impaired public health. The ARGs allows bacteria to develop resistance towards antibiotics while ARB render antibiotics ineffective. Existing drinking water treatment technologies eg adsorption, ozonation and chlorination, have shown different levels of potency in the removal of conventional water pollutant. These technologies, which operating principles is based on oxidation or mass transfer, have been explored to gained an insight into their efficiency in the removal of ARB ARGs in water treatment. In general, methods that functions by mass transfer of the ARB ARGs is more effectively than bacteria oxidation. Consequently, adsorption technology was chosen using common metal oxide adsorbents. The adsorbents ZnOAg CeO2 and Al2O3 were synthesized via the self propagation combustion method. This method was selected because of the purity of the final product and the energy requirement. One of the challenges in the removal of ARB in drinking water is the release of ARGs. To address this concern, five different ZnOAg heterostructures were synthesized for the removal of Enterococci faecium. ZnOAg was chosen because of the bactericidal and bacteriostatic characteristics. The study revealed that the concentration of the precursors influences the microstructures of the adsorbents; however, it did not significantly affect the adsorption efficiency. The maximum adsorption capacity q34.11 CFUg was obtained for Ag1Zn3.5. The kinetic studies revealed that Ag1Zn1 and Ag1Zn2 adsorbents agreed to the pseudofirst-order kinetic equation and adsorbents Ag2Zn1 Ag3.5Zn1 and Ag1Zn3.5 agreed to the pseudo-second-order kinetic equation. Initial tap-water pH range was beneficial for the adsorption and the pH of the treated tap-water was within the WHO tap water recommendation 6.5 – 8.5 whereas the effect of ionic strength, anionic and cationic interference was insignificant in the adsorption of MDREF onto the different heterostructure. Interestingly the MDREF could retain its cell membrane integrity and resistance genes, suggesting that surface adsorption was the primary mechanism for the removal. Cerium IV oxide CeO2 was selected because of high adsorption towards phosphate, backbone base for DNA. To prevent the problem of antibiotic resistance, we have synthesized a CeO2 adsorbent that exhibit highly positive character in a wide pH range, via the simple self-propagation combustion protocol, for the removal of free DNA harboring antibiotic resistance genes. Molecular characterization of the extracted genes showed that the sizes for E. coli and inherent gyrB genes are 147 and 460 bp with a purity between 19 2.0. The XRD SEM TEM, and PZC results of the as-synthesized CeO2 showed an agglomerate of pure cubic-faced centered material and highly crystalline, with a net charge at pH 6.2. Experimental results revealed that the reaction proceeded via pseudo first-order kinetic, and it is governed by electrostatic attraction. The free- DNA solution pH electrolyte, and competing ions impacted on the adsorption process. Further experimental results showed that the as-synthesized CeO2 adsorbent has the potential to be used for the removal of free DNA harboring ARGs from tap-water even under oxic conditions. Alumina Al2O3 is an abundant adsorbent that has also shown high removal capacity towards phosphate. The highly pure synthesized Al2O3 adsorbent exhibit fluid-like behaviour under Scanning Electron Microscope SEM. The XRD pattern corresponds to αAl2O3. The adsorption kinetics was described by pseudo second orderadsorption capacity 11.7 μgg implying chemisorption, which agrees with the electrostatic force of attraction caused by opposing ions. This result was evident by the effect of different ions in the tap water. The synthesized α-Al2O3 has the potential for the removal of cell free DNA harbouring multiply resistant genes. , Thesis (MSc) -- Faculty of Science and Agriculture, 2022
- Full Text:
- Authors: Tobechukwu, Anthony Eric
- Date: 2022
- Subjects: Metallic oxides , DNA -- Synthesis , Drinking water
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/27787 , vital:69494
- Description: Access to cleaner water is essential to human health. The incidence of Antibiotic Resistant Bacteria ARB in drinking water and subsequent proliferation of Antibiotic Resistance Genes ARGs in drinking water is a concern for public health. The free DNA cassette harboring antibiotic resistance gene in drinking water has been linked with impaired public health. The ARGs allows bacteria to develop resistance towards antibiotics while ARB render antibiotics ineffective. Existing drinking water treatment technologies eg adsorption, ozonation and chlorination, have shown different levels of potency in the removal of conventional water pollutant. These technologies, which operating principles is based on oxidation or mass transfer, have been explored to gained an insight into their efficiency in the removal of ARB ARGs in water treatment. In general, methods that functions by mass transfer of the ARB ARGs is more effectively than bacteria oxidation. Consequently, adsorption technology was chosen using common metal oxide adsorbents. The adsorbents ZnOAg CeO2 and Al2O3 were synthesized via the self propagation combustion method. This method was selected because of the purity of the final product and the energy requirement. One of the challenges in the removal of ARB in drinking water is the release of ARGs. To address this concern, five different ZnOAg heterostructures were synthesized for the removal of Enterococci faecium. ZnOAg was chosen because of the bactericidal and bacteriostatic characteristics. The study revealed that the concentration of the precursors influences the microstructures of the adsorbents; however, it did not significantly affect the adsorption efficiency. The maximum adsorption capacity q34.11 CFUg was obtained for Ag1Zn3.5. The kinetic studies revealed that Ag1Zn1 and Ag1Zn2 adsorbents agreed to the pseudofirst-order kinetic equation and adsorbents Ag2Zn1 Ag3.5Zn1 and Ag1Zn3.5 agreed to the pseudo-second-order kinetic equation. Initial tap-water pH range was beneficial for the adsorption and the pH of the treated tap-water was within the WHO tap water recommendation 6.5 – 8.5 whereas the effect of ionic strength, anionic and cationic interference was insignificant in the adsorption of MDREF onto the different heterostructure. Interestingly the MDREF could retain its cell membrane integrity and resistance genes, suggesting that surface adsorption was the primary mechanism for the removal. Cerium IV oxide CeO2 was selected because of high adsorption towards phosphate, backbone base for DNA. To prevent the problem of antibiotic resistance, we have synthesized a CeO2 adsorbent that exhibit highly positive character in a wide pH range, via the simple self-propagation combustion protocol, for the removal of free DNA harboring antibiotic resistance genes. Molecular characterization of the extracted genes showed that the sizes for E. coli and inherent gyrB genes are 147 and 460 bp with a purity between 19 2.0. The XRD SEM TEM, and PZC results of the as-synthesized CeO2 showed an agglomerate of pure cubic-faced centered material and highly crystalline, with a net charge at pH 6.2. Experimental results revealed that the reaction proceeded via pseudo first-order kinetic, and it is governed by electrostatic attraction. The free- DNA solution pH electrolyte, and competing ions impacted on the adsorption process. Further experimental results showed that the as-synthesized CeO2 adsorbent has the potential to be used for the removal of free DNA harboring ARGs from tap-water even under oxic conditions. Alumina Al2O3 is an abundant adsorbent that has also shown high removal capacity towards phosphate. The highly pure synthesized Al2O3 adsorbent exhibit fluid-like behaviour under Scanning Electron Microscope SEM. The XRD pattern corresponds to αAl2O3. The adsorption kinetics was described by pseudo second orderadsorption capacity 11.7 μgg implying chemisorption, which agrees with the electrostatic force of attraction caused by opposing ions. This result was evident by the effect of different ions in the tap water. The synthesized α-Al2O3 has the potential for the removal of cell free DNA harbouring multiply resistant genes. , Thesis (MSc) -- Faculty of Science and Agriculture, 2022
- Full Text:
Backpack carriage and musculoskeletal pain among primary school learners in King Williams Town, South Africa
- Authors: Moni, Busisiwe
- Date: 2022
- Subjects: Backache -- Prevention , Pediatric rheumatology , Pain -- Treatment
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/26984 , vital:66208
- Description: BACKGROUND: There is rising concern that kids are walking to school with too much weight on their backs. The weight of schoolchildren's backpacks is a persistent and divisive topic in the fields of education and health. Children who carry heavy backpacks are more likely to have back discomfort and musculoskeletal pain, which raises their risk of developing chronic back pain as adults. There is little research on this topic among South African school-age youngsters. The goal of the study was to find out how common low back and other musculoskeletal complaints were among primary school students who used a school bag. RESEARCH AIM: The purpose of the study was to identify the prevalence and location of musculoskeletal discomfort in primary school students in King William's Town, South Africa, and describe their link with backpack use. METHOD: In King Williams Town, Eastern Cape, South Africa, a descriptive cross-sectional survey was conducted among elementary school students between the ages of 9 and 14 years old. A systematic questionnaire and the Cornell Musculoskeletal Questionnaire were used to gather the data (body chart). Age, gender, and schoolbag details (style of backpack, carrying technique, and carrying time) were collected. The learner's waist, hip, and bag circumferences were all measured. On the data, descriptive and interferential statistics were used. FINDINGS: The study's conclusions showed that students' musculoskeletal pain was brought on by the weight of their backpacks or school bags. The majority of the students (89.8percent) complain about the difficulty they experience when lugging their heavy backpacks to class. In addition, 83.4percent of the students had bags that weighed more than 10percent of their body weight. Additionally, a sizable percentage of students (89.8percent) carry a hefty bag to school every day and report feeling fatigued while doing so (90.8percent). 90percent of the students reported feeling discomfort before, during, or after carrying their schoolbags. This pain is related with carrying a schoolbag. The majority of students (53.5percent) felt that their daily pain interferes with their ability to study. In order of severity, the majority of students report having discomfort in their shoulders (82.7percent), lower back (59.9percent), neck (47.0percent), upper back (40.7percent), and upper arm (16.4percent). CONCLUSION: According to the study, there is a link between students' musculoskeletal pain and carrying hefty backpacks or school bags. The shoulders are where musculoskeletal discomfort is most frequently felt. The study supported the findings of the majority of studies cited in the literature that most schoolchildren suffer from musculoskeletal pain because the weight of their backpacks is excessive compared to their size, weight, and age. RECOMMENDATIONS: The study produced a number of recommendations, including that the Department of Basic Education inform teachers, students, parents, guardians, and other key stakeholders about the effects of heavy backpacks or schoolbags on students' musculoskeletal pain. For students' convenience and to lighten the load on their school bags, locker rooms should be available. Teachers, parents, and guardians should also pay attention to the school bags that students are using to make sure that the weight is appropriate for their age and body size. , Thesis (MPA) -- Faculty of Health Sciences, 2022
- Full Text:
- Authors: Moni, Busisiwe
- Date: 2022
- Subjects: Backache -- Prevention , Pediatric rheumatology , Pain -- Treatment
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/26984 , vital:66208
- Description: BACKGROUND: There is rising concern that kids are walking to school with too much weight on their backs. The weight of schoolchildren's backpacks is a persistent and divisive topic in the fields of education and health. Children who carry heavy backpacks are more likely to have back discomfort and musculoskeletal pain, which raises their risk of developing chronic back pain as adults. There is little research on this topic among South African school-age youngsters. The goal of the study was to find out how common low back and other musculoskeletal complaints were among primary school students who used a school bag. RESEARCH AIM: The purpose of the study was to identify the prevalence and location of musculoskeletal discomfort in primary school students in King William's Town, South Africa, and describe their link with backpack use. METHOD: In King Williams Town, Eastern Cape, South Africa, a descriptive cross-sectional survey was conducted among elementary school students between the ages of 9 and 14 years old. A systematic questionnaire and the Cornell Musculoskeletal Questionnaire were used to gather the data (body chart). Age, gender, and schoolbag details (style of backpack, carrying technique, and carrying time) were collected. The learner's waist, hip, and bag circumferences were all measured. On the data, descriptive and interferential statistics were used. FINDINGS: The study's conclusions showed that students' musculoskeletal pain was brought on by the weight of their backpacks or school bags. The majority of the students (89.8percent) complain about the difficulty they experience when lugging their heavy backpacks to class. In addition, 83.4percent of the students had bags that weighed more than 10percent of their body weight. Additionally, a sizable percentage of students (89.8percent) carry a hefty bag to school every day and report feeling fatigued while doing so (90.8percent). 90percent of the students reported feeling discomfort before, during, or after carrying their schoolbags. This pain is related with carrying a schoolbag. The majority of students (53.5percent) felt that their daily pain interferes with their ability to study. In order of severity, the majority of students report having discomfort in their shoulders (82.7percent), lower back (59.9percent), neck (47.0percent), upper back (40.7percent), and upper arm (16.4percent). CONCLUSION: According to the study, there is a link between students' musculoskeletal pain and carrying hefty backpacks or school bags. The shoulders are where musculoskeletal discomfort is most frequently felt. The study supported the findings of the majority of studies cited in the literature that most schoolchildren suffer from musculoskeletal pain because the weight of their backpacks is excessive compared to their size, weight, and age. RECOMMENDATIONS: The study produced a number of recommendations, including that the Department of Basic Education inform teachers, students, parents, guardians, and other key stakeholders about the effects of heavy backpacks or schoolbags on students' musculoskeletal pain. For students' convenience and to lighten the load on their school bags, locker rooms should be available. Teachers, parents, and guardians should also pay attention to the school bags that students are using to make sure that the weight is appropriate for their age and body size. , Thesis (MPA) -- Faculty of Health Sciences, 2022
- Full Text:
Development and in vitro biological studies of polymer-based wound dressings with a high haemostatic ability for the management of wounds
- Nqoro, Xhamla https://orcid.org/0000-0002-2065-3629
- Authors: Nqoro, Xhamla https://orcid.org/0000-0002-2065-3629
- Date: 2022
- Subjects: Wound healing , Surgical wound infections , Wound treatment equipment industry
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/27970 , vital:71404
- Description: Wounds are usually accompanied by complications such as excessive bleeding and bacteria invasion. The design of wound dressings that rapidly stop excessive bleeding and inhibit bacterial invasion is crucial to promoting accelerated wound healing. To meet the abovementioned requirements in wound dressings, topical gels were prepared from sodium alginate SA and carboxymethylcellulose CMC. The wound dressings were loaded with an antifibrinolytic agent, tranexamic acid TA, essential oils, and a variety of metal-based nanoparticles, and carbon-based biomaterials. The scanning electron microscopy SEM and X-ray diffraction XRD confirmed the successful formation of the nanoparticles. The prepared formulations exhibited in vitro drug release kinetics that best fitted with the Korsmeyer-Peppas model. These gels exhibited good spreadability and viscosity, showing a shear-thinning behaviour with pH between 6.7 and 7.3, signifying suitability for skin application and ease of application. The prepared topical gels exhibited significant antibacterial effects against gram-negative and gram-positive strains of bacteria. SA EO-based formulations showed high antibacterial activity across all bacterial strains, followed by SA-based formulations compared to CMC-based formulations, which exhibited moderate antibacterial activity. Moreover, the prepared gels showed good cytocompatibility, promoted cell proliferation, and exhibited 80 percent wound closure on day 3 compared to the untreated group, which showed a 38 percent wound reduction in vitro. Excellent blood clotting properties were observed with CMC-based gels compared to other formulations. However, all the prepared formulations exhibited outstanding blood clotting ability compared to the control, showing that they can promote rapid blood coagulation. The features presented by the prepared gels reveal that they are suitable for rapid wound healing , Thesis (PhD) -- Faculty of Science and Agriculture, 2022
- Full Text:
- Authors: Nqoro, Xhamla https://orcid.org/0000-0002-2065-3629
- Date: 2022
- Subjects: Wound healing , Surgical wound infections , Wound treatment equipment industry
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/27970 , vital:71404
- Description: Wounds are usually accompanied by complications such as excessive bleeding and bacteria invasion. The design of wound dressings that rapidly stop excessive bleeding and inhibit bacterial invasion is crucial to promoting accelerated wound healing. To meet the abovementioned requirements in wound dressings, topical gels were prepared from sodium alginate SA and carboxymethylcellulose CMC. The wound dressings were loaded with an antifibrinolytic agent, tranexamic acid TA, essential oils, and a variety of metal-based nanoparticles, and carbon-based biomaterials. The scanning electron microscopy SEM and X-ray diffraction XRD confirmed the successful formation of the nanoparticles. The prepared formulations exhibited in vitro drug release kinetics that best fitted with the Korsmeyer-Peppas model. These gels exhibited good spreadability and viscosity, showing a shear-thinning behaviour with pH between 6.7 and 7.3, signifying suitability for skin application and ease of application. The prepared topical gels exhibited significant antibacterial effects against gram-negative and gram-positive strains of bacteria. SA EO-based formulations showed high antibacterial activity across all bacterial strains, followed by SA-based formulations compared to CMC-based formulations, which exhibited moderate antibacterial activity. Moreover, the prepared gels showed good cytocompatibility, promoted cell proliferation, and exhibited 80 percent wound closure on day 3 compared to the untreated group, which showed a 38 percent wound reduction in vitro. Excellent blood clotting properties were observed with CMC-based gels compared to other formulations. However, all the prepared formulations exhibited outstanding blood clotting ability compared to the control, showing that they can promote rapid blood coagulation. The features presented by the prepared gels reveal that they are suitable for rapid wound healing , Thesis (PhD) -- Faculty of Science and Agriculture, 2022
- Full Text:
Evaluation of functionalized silver and silica nanoparticles for the removal of deoxyribonucleic acid conveying antibiotics resistance genes from water
- Authors: Ezeuko, Adaora Stella
- Date: 2022
- Subjects: DNA , Silica , Water
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/27765 , vital:69414
- Description: Antibiotic resistance genes ARGs are recognized as a serious public health emergency linked to extensive use of antibiotics by humans and animals as a prophylactic agent that treats and prevents infections. The occurrence of high concentrations being identified in wastewater treatment plants, rivers, etc is due to untreated effluents being discharged from households, hospitals, agriculture, and pharmaceutical industries. The application of adequate treatment techniques and material for the removal of bacteria DNA conveying ARGs from the effluents before their release to the environment cannot be overemphasized. Adsorption techniques seem to be effective due to their easy design, operation, and ability to regenerate adsorbents for use without producing toxic by-products. This concept was employed for the removal of bacteria DNA conveying ARGs from simulated aqueous solution, effluents from hospital, river and WWTPs using silver and silica metallic nanoparticles. This thesis investigated the effectiveness of metallic nanoparticles containing silver AgNPs and mesoporous silica nanoparticles MSNPs as well as magnetite Fe3O4 functionalized with 4 4hydroxyphenyl 2 262-terpyridine onto their surface, for the removal of bacteria DNA conveying antibiotic resistance genes from water samples from hospitals, river, and wastewater treatment plants WWTPs. Silver nanoparticles AgNPs of different molar concentrations 0.1M, 0.5M and 1.0 M and mesoporous silica nanoparticles MSNPs adsorbents were successfully synthesized in their original states and surface functionalization achieved by incorporating magnetite Fe3O4 and 4 4 hydroxyphenyl 2 2 6 2 terpyridine on the silver AgNPs Fe3O4 and silica MSNPs TPPY surfaces respectively. Their effectiveness as adsorbent for the removal of bacteria DNA conveying ARGs from aqueous solutions and real water/wastewater samples were investigated. The DNA uptake by the as-synthesized AgNPs and MSNPs were compared to the functionalized AgNPs Fe3O4 and MSNPsTPPY by determining the adsorbents with the highest removal efficiencies. All as synthesized and functionalized adsorbents were characterized by SEM, EDX, FTIR, XRD, UV spectroscopy and PZC before the removal process. The extraction of genomic DNA from antibiotic-resistant Enterococcus faecium and Vibrio parahaemolyticus was successfully achieved via the boiling method. Antibiotic susceptibility test was conducted using the disk diffusion method before the commencement of genomic DNA extraction. Molecular characterization via gel electrophoresis confirmed the presence of resistance genes at different base pairs. Adsorption batch experiment were investigated, and the best optimum parameters were evaluated through the influence of pH, contact time, initial DNA concentration, adsorbent dose, and competitive ions for each sorption process. The rate determining step were determined by fitting kinetic models such as Natarajan and Khalaf first order, pseudo first order, pseudo second order, Elovich model to experimental data. Also, the adsorption mechanisms determining adsorption equilibrium were investigated by fitting Freundlich, Langmuir and Sips model into the experimental data. The application of AgNPsFe3O4 nanocomposite and MSNPsTPPY for the removal of bacteria DNA demonstrated much enhancement for DNA uptake than the as-synthesized AgNPs and MSNPs materials. The incorporation of magnetite and 4 4hydroxyphenyl 2 2 6 2-terpyridine onto AgNPs and MSNPs significantly enhanced the binding affinity towards the removal the bacteria DNA via strong electrostatic attraction between the active sites on the adsorbent and the negative DNA molecules. Finally, high adsorption capacities were recorded with AgNPsFe3O4 nanocomposite and MSNPsTPPY compared to AgNPs and MSNPs with chaotropic salts. The kinetic adsorption models were mostly best fitted by the pseudo-second order and Elovich models while the adsorption equilibrium was best described by Langmuir and Sips isotherm models. MSNPs with different chaotropic salts, AgNPsFe3O4 nanocomposite and MSNPsTPPY also proved its effectiveness in DNA removal not only in the simulated aqueous solution but in three different real life water samples obtained from Cofimvaba hospital, Ndevana river and Uitenhage WWTPs. High adsorption efficiencies above 90 percent were achieved during the removal of DNA in all the three real water samples. Therefore, application of these adsorbents for the removal of bacteria DNA conveying ARGs may be a promising option that would tackle the consequences of consuming ARGs infected water globally. , Thesis (MSc) -- Faculty of Science and Agriculture, 2022
- Full Text:
- Authors: Ezeuko, Adaora Stella
- Date: 2022
- Subjects: DNA , Silica , Water
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/27765 , vital:69414
- Description: Antibiotic resistance genes ARGs are recognized as a serious public health emergency linked to extensive use of antibiotics by humans and animals as a prophylactic agent that treats and prevents infections. The occurrence of high concentrations being identified in wastewater treatment plants, rivers, etc is due to untreated effluents being discharged from households, hospitals, agriculture, and pharmaceutical industries. The application of adequate treatment techniques and material for the removal of bacteria DNA conveying ARGs from the effluents before their release to the environment cannot be overemphasized. Adsorption techniques seem to be effective due to their easy design, operation, and ability to regenerate adsorbents for use without producing toxic by-products. This concept was employed for the removal of bacteria DNA conveying ARGs from simulated aqueous solution, effluents from hospital, river and WWTPs using silver and silica metallic nanoparticles. This thesis investigated the effectiveness of metallic nanoparticles containing silver AgNPs and mesoporous silica nanoparticles MSNPs as well as magnetite Fe3O4 functionalized with 4 4hydroxyphenyl 2 262-terpyridine onto their surface, for the removal of bacteria DNA conveying antibiotic resistance genes from water samples from hospitals, river, and wastewater treatment plants WWTPs. Silver nanoparticles AgNPs of different molar concentrations 0.1M, 0.5M and 1.0 M and mesoporous silica nanoparticles MSNPs adsorbents were successfully synthesized in their original states and surface functionalization achieved by incorporating magnetite Fe3O4 and 4 4 hydroxyphenyl 2 2 6 2 terpyridine on the silver AgNPs Fe3O4 and silica MSNPs TPPY surfaces respectively. Their effectiveness as adsorbent for the removal of bacteria DNA conveying ARGs from aqueous solutions and real water/wastewater samples were investigated. The DNA uptake by the as-synthesized AgNPs and MSNPs were compared to the functionalized AgNPs Fe3O4 and MSNPsTPPY by determining the adsorbents with the highest removal efficiencies. All as synthesized and functionalized adsorbents were characterized by SEM, EDX, FTIR, XRD, UV spectroscopy and PZC before the removal process. The extraction of genomic DNA from antibiotic-resistant Enterococcus faecium and Vibrio parahaemolyticus was successfully achieved via the boiling method. Antibiotic susceptibility test was conducted using the disk diffusion method before the commencement of genomic DNA extraction. Molecular characterization via gel electrophoresis confirmed the presence of resistance genes at different base pairs. Adsorption batch experiment were investigated, and the best optimum parameters were evaluated through the influence of pH, contact time, initial DNA concentration, adsorbent dose, and competitive ions for each sorption process. The rate determining step were determined by fitting kinetic models such as Natarajan and Khalaf first order, pseudo first order, pseudo second order, Elovich model to experimental data. Also, the adsorption mechanisms determining adsorption equilibrium were investigated by fitting Freundlich, Langmuir and Sips model into the experimental data. The application of AgNPsFe3O4 nanocomposite and MSNPsTPPY for the removal of bacteria DNA demonstrated much enhancement for DNA uptake than the as-synthesized AgNPs and MSNPs materials. The incorporation of magnetite and 4 4hydroxyphenyl 2 2 6 2-terpyridine onto AgNPs and MSNPs significantly enhanced the binding affinity towards the removal the bacteria DNA via strong electrostatic attraction between the active sites on the adsorbent and the negative DNA molecules. Finally, high adsorption capacities were recorded with AgNPsFe3O4 nanocomposite and MSNPsTPPY compared to AgNPs and MSNPs with chaotropic salts. The kinetic adsorption models were mostly best fitted by the pseudo-second order and Elovich models while the adsorption equilibrium was best described by Langmuir and Sips isotherm models. MSNPs with different chaotropic salts, AgNPsFe3O4 nanocomposite and MSNPsTPPY also proved its effectiveness in DNA removal not only in the simulated aqueous solution but in three different real life water samples obtained from Cofimvaba hospital, Ndevana river and Uitenhage WWTPs. High adsorption efficiencies above 90 percent were achieved during the removal of DNA in all the three real water samples. Therefore, application of these adsorbents for the removal of bacteria DNA conveying ARGs may be a promising option that would tackle the consequences of consuming ARGs infected water globally. , Thesis (MSc) -- Faculty of Science and Agriculture, 2022
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Evaluation of the extent of the public programming by Eastern Cape Provincial Archives and Records Services
- Tshebi, Siyathemba Mboneleli https://orcid.org/0000-0003-2833-9842
- Authors: Tshebi, Siyathemba Mboneleli https://orcid.org/0000-0003-2833-9842
- Date: 2022
- Subjects: Grahamstown (South Africa) , Eastern Cape (South Africa) , South Africa
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/27663 , vital:69381
- Description: Public programming initiatives are an integral part of archival operations worldwide as they support the greater use of archival records by attracting prospective users and sponsors. Public programming comprises a sequence of strategies for publicizing and marketing archives to the public, which are crucial in archival operations as they promote better use of archives and records. South African National Archives and Records. Services depends on provisions of the National Archives of South Africa Act of 1996 in its strive to promote access and use of archives as sources of information. . unknown and therefore not used to their maximal utility value, likely due to low-key and ineffective archival public programming initiatives. Consequently, the primary purpose of this research study was to evaluate the extent of public programming strategies used by the Eastern Cape Provincial Archives and Records Services (EAPARS). The study used a mixed-method, embracing qualitative and quantitative research approaches. As an integrated record-keeping framework, Record Continuum was used as a theoretical framework to guide the study. Sixteen copies of questionnaire were distributed among staff and users ECPARS. The research uncovered that ECPARS does not have specific regulations and policies on public programming, but relies on the more general provisions of the Provincial Archives and Records Services Act of 1996 as a guiding instrument in executing its public programming operations. The study further revealed various factors, such as the limited budget and inadequate staff, as key hindering factors in the smooth execution of archival public programming plans by ECPARS. The study recommends a review and update by ECPARS of how it can specifically effectively implement the provisions of the 1996 Act to the modern ways of taking public archives to the people. The research also recommends that ECPARS engage additional staff or outsourced services to strengthen its the public programming of its archival resources and services workforce for Eastern Cape Provincial Archives and Records. The study concludes that improved financial resources, use of ICTs, and coherent policy framework, and public awareness creation and education can facilitate better to acquire, widen access to use of its archives. Keyword , Thesis (MLIS) -- Faculty of Social Sciences and Humanities, 2022
- Full Text:
- Authors: Tshebi, Siyathemba Mboneleli https://orcid.org/0000-0003-2833-9842
- Date: 2022
- Subjects: Grahamstown (South Africa) , Eastern Cape (South Africa) , South Africa
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/27663 , vital:69381
- Description: Public programming initiatives are an integral part of archival operations worldwide as they support the greater use of archival records by attracting prospective users and sponsors. Public programming comprises a sequence of strategies for publicizing and marketing archives to the public, which are crucial in archival operations as they promote better use of archives and records. South African National Archives and Records. Services depends on provisions of the National Archives of South Africa Act of 1996 in its strive to promote access and use of archives as sources of information. . unknown and therefore not used to their maximal utility value, likely due to low-key and ineffective archival public programming initiatives. Consequently, the primary purpose of this research study was to evaluate the extent of public programming strategies used by the Eastern Cape Provincial Archives and Records Services (EAPARS). The study used a mixed-method, embracing qualitative and quantitative research approaches. As an integrated record-keeping framework, Record Continuum was used as a theoretical framework to guide the study. Sixteen copies of questionnaire were distributed among staff and users ECPARS. The research uncovered that ECPARS does not have specific regulations and policies on public programming, but relies on the more general provisions of the Provincial Archives and Records Services Act of 1996 as a guiding instrument in executing its public programming operations. The study further revealed various factors, such as the limited budget and inadequate staff, as key hindering factors in the smooth execution of archival public programming plans by ECPARS. The study recommends a review and update by ECPARS of how it can specifically effectively implement the provisions of the 1996 Act to the modern ways of taking public archives to the people. The research also recommends that ECPARS engage additional staff or outsourced services to strengthen its the public programming of its archival resources and services workforce for Eastern Cape Provincial Archives and Records. The study concludes that improved financial resources, use of ICTs, and coherent policy framework, and public awareness creation and education can facilitate better to acquire, widen access to use of its archives. Keyword , Thesis (MLIS) -- Faculty of Social Sciences and Humanities, 2022
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In Silico analysis of Spermidine, Spermine and Putrescine interaction with selected heat shock proteins from Plasmodium falciparum 3D7 towards malaria drug development
- Authors: Godlo, Sesethu
- Date: 2022
- Subjects: Heat shock proteins , Malaria vaccine , Plasmodium falciparum
- Language: English
- Type: Master'stheses , text
- Identifier: http://hdl.handle.net/10353/27709 , vital:69395
- Description: Malaria is a mosquito-borne disease that affects around half of the world's population. It is one of the most common parasite infections that endangers human life. One of the most serious issues in malaria therapy is the emergence and spread of antimalarial drug-resistant Plasmodium parasites. This is due to the Plasmodium parasite's constant evolution and development of new methods of surviving medication toxicity. Studies of antimalarial drug development have been focused on polyamine biosynthesis by targeting precursors such as ornithine decarboxylase, adenosylmethionine decarboxylase, and spermidine synthase and protein-protein interactions between Plasmodium falciparum chaperones spotting out Hsp90, Hsp70, and Hsp40 as potential targets with little attention being paid to the interaction between polyamines and molecular chaperones. Therefore, this study seeks to identify interactions between polyamines and molecular chaperones present in the malarial parasite Plasmodium falciparum 3D7. The identification of these interactions might lead to the design of effective drugs to treat and eliminate malaria. Using computational analysis, this study aims to find interactions between polyamines and molecular chaperones found in the malarial parasite Plasmodium falciparum 3D7. The sequences of the selected heat proteins retrieved, and the 3D structures predicted and validated. These structures were docked with polyamines retrieved from PubChem and MD simulations on the docked interactions were performed. Docking revealed common amino acid residues with hydrogen bonds and salt bridges between all receptors and ligands, with glutamine and aspartic acid standing out. MD simulations revealed that when HSP20 and HSP40 transport the ligands, they pop up or are released too quickly. However, HSP60, HSP70, and HSP90 provide optimism since the ligands remain attached to the proteins for a specific amount of time. To further understand and confirm these interactions wet laboratory studies may be carried out in future. , Thesis (MSci) -- Faculty of Science and Agriculture, 2022
- Full Text:
- Authors: Godlo, Sesethu
- Date: 2022
- Subjects: Heat shock proteins , Malaria vaccine , Plasmodium falciparum
- Language: English
- Type: Master'stheses , text
- Identifier: http://hdl.handle.net/10353/27709 , vital:69395
- Description: Malaria is a mosquito-borne disease that affects around half of the world's population. It is one of the most common parasite infections that endangers human life. One of the most serious issues in malaria therapy is the emergence and spread of antimalarial drug-resistant Plasmodium parasites. This is due to the Plasmodium parasite's constant evolution and development of new methods of surviving medication toxicity. Studies of antimalarial drug development have been focused on polyamine biosynthesis by targeting precursors such as ornithine decarboxylase, adenosylmethionine decarboxylase, and spermidine synthase and protein-protein interactions between Plasmodium falciparum chaperones spotting out Hsp90, Hsp70, and Hsp40 as potential targets with little attention being paid to the interaction between polyamines and molecular chaperones. Therefore, this study seeks to identify interactions between polyamines and molecular chaperones present in the malarial parasite Plasmodium falciparum 3D7. The identification of these interactions might lead to the design of effective drugs to treat and eliminate malaria. Using computational analysis, this study aims to find interactions between polyamines and molecular chaperones found in the malarial parasite Plasmodium falciparum 3D7. The sequences of the selected heat proteins retrieved, and the 3D structures predicted and validated. These structures were docked with polyamines retrieved from PubChem and MD simulations on the docked interactions were performed. Docking revealed common amino acid residues with hydrogen bonds and salt bridges between all receptors and ligands, with glutamine and aspartic acid standing out. MD simulations revealed that when HSP20 and HSP40 transport the ligands, they pop up or are released too quickly. However, HSP60, HSP70, and HSP90 provide optimism since the ligands remain attached to the proteins for a specific amount of time. To further understand and confirm these interactions wet laboratory studies may be carried out in future. , Thesis (MSci) -- Faculty of Science and Agriculture, 2022
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Indifference and Epistemic Injustice: Phenomenological Reflections
- Roos,R
- Authors: Roos,R
- Date: 2022
- Subjects: Epistemic logic , Virtue epistemology
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/27150 , vital:66324
- Description: The proposed research seeks to address salient questions concerning the relation of indifference and epistemic injustice. This involves questions dealing with the necessary and sufficient conditions for indifference; how it arises through our conditions of lived experience and how it extends to the production, maintenance and engagement with knowledge. In short, to what extent does indifference play a role in epistemic injustice? Conversely, to what extent does epistemic injustice relate to indifference? The proposed research will explore major concepts of the relation between indifference and epistemic injustice. The research will settle on a phenomenological approach. Its claim is that major contemporary concepts of indifference and injustice can be critically informed by a phenomenological analysis of these concepts. Thus, it will attempt to analyse the relation of indifference and injustice from within the context of our lived conditions of experience. Ultimately, this research will try to locate the similarities between the experience of indifference and the act of epistemic injustice. , Thesis (MSoc) -- Faculty of Social Sciences and Humanities, 2022
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- Authors: Roos,R
- Date: 2022
- Subjects: Epistemic logic , Virtue epistemology
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/27150 , vital:66324
- Description: The proposed research seeks to address salient questions concerning the relation of indifference and epistemic injustice. This involves questions dealing with the necessary and sufficient conditions for indifference; how it arises through our conditions of lived experience and how it extends to the production, maintenance and engagement with knowledge. In short, to what extent does indifference play a role in epistemic injustice? Conversely, to what extent does epistemic injustice relate to indifference? The proposed research will explore major concepts of the relation between indifference and epistemic injustice. The research will settle on a phenomenological approach. Its claim is that major contemporary concepts of indifference and injustice can be critically informed by a phenomenological analysis of these concepts. Thus, it will attempt to analyse the relation of indifference and injustice from within the context of our lived conditions of experience. Ultimately, this research will try to locate the similarities between the experience of indifference and the act of epistemic injustice. , Thesis (MSoc) -- Faculty of Social Sciences and Humanities, 2022
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Preparation, characterization, and in vitro evaluation of polymer-based wound dressings for the management of chronic wounds
- Authors: Alven, Sibusiso
- Date: 2022
- Subjects: Toxicity testing -- In vitro , Wound healing , Chronic diseases
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/27992 , vital:71414
- Description: Microbial infections are responsible for the retarded recovery process of chronic wounds. Polymer-based scaffolds possess features suitable for the treatment of chronic injuries. However, these scaffolds are commonly encapsulated with therapeutic agents to enhance their biological activities, including antibacterial efficacy. In this research, two types of polymer-based scaffolds were formulated and evaluated as effective formulations for the treatment of chronic wounds: sponges and topical gels. Sponges were formulated from cross-linking of gelatin and PEG. Ag nanoparticles and metronidazole were incorporated into the sponges to improve their antibacterial activity. Topical gels were loaded with essential oils and Ag nanoparticles and prepared from CMC and poloxamer. The prepared sponges and topical gels were evaluated using various analysis and characterization techniques. SEM/EDX, FTIR, and TGA were employed to characterize gelatin/PEG hybrid sponges followed by porosity, in vitro biodegradability, cytotoxicity, and antibacterial studies. FTIR, SEM/EDX, and TGA confirmed their physicochemical properties and successful fabrication of sponges loaded with metronidazole and Ag nanoparticles. The sponges were biodegradable, indicating their capability to induce skin regeneration. The drug release studies showed a rapid release of metronidazole 28.32-71.97 percent from the sponges over the first hour, followed by a sustained drug release. The Ag nanoparticles were released in a sustained manner, suggesting that these sponges can rapidly destroy bacteria and inhibit persisting bacterial infections as well as protect the lesion bed from further bacteria infections. The in vitro antibacterial studies of sponges displayed superior antibacterial activity against most of the Gram-negative and Gram-positive bacteria strains commonly found in chronic wound infections with a MIC value of 15.625 μg/mL. In vitro cytotoxicity experiments revealed excellent biocompatibility with a percent cell viability of more than 70 percent. The in vitro wound scratch healing assay exhibited that the sponges encapsulated with only metronidazole promoted high cell migration than the dual drug-loaded sponges and untreated cells, suggesting its potential to quicken the wound healing process. CMC/Poloxamer topical gels were also characterized by FTIR, followed by pH, viscosity, spreadability, cytotoxicity, and antibacterial studies. FTIR showed successful preparation of CMC/Poloxamer topical gels loaded with essential oils and Ag nanoparticles. The topical gels exhibited pH in the range of 5.20-6.68, spreadability between 5.4 and 5.9 cm, and viscosity ranged from 216 to 1200 cP at 50 rpm and 210–858 cP at 100 rpm. The in vitro drug release studies demonstrated that Ag nanoparticles were released from the topical gels in a sustained manner. Most formulated topical gels demonstrated superior antimicrobial efficacy against Gram-positive and Gram-negative bacteria strains than the blank gel and controls. The cytotoxicity analysis displayed more than 90.83 percent cell viability for the topical gels, revealing excellent biocompatibility. The outcomes revealed that the topical gels enriched with essential oils lavender and tea tree and Ag nanoparticles and sponges incorporated with metronidazole and Ag nanoparticles are potential wound dressing scaffolds that can be employed for the treatment of chronic infected injuries. The in vitro wound healing experiments showed that the HaCaT cells cultured with gels co-enriched with lavender oil and Ag nanoparticles possessed a higher rate of closure in comparison to the untreated cells for 96 hours. , Thesis (PhD) -- Faculty of Science and Agriculture, 2022
- Full Text:
- Authors: Alven, Sibusiso
- Date: 2022
- Subjects: Toxicity testing -- In vitro , Wound healing , Chronic diseases
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/27992 , vital:71414
- Description: Microbial infections are responsible for the retarded recovery process of chronic wounds. Polymer-based scaffolds possess features suitable for the treatment of chronic injuries. However, these scaffolds are commonly encapsulated with therapeutic agents to enhance their biological activities, including antibacterial efficacy. In this research, two types of polymer-based scaffolds were formulated and evaluated as effective formulations for the treatment of chronic wounds: sponges and topical gels. Sponges were formulated from cross-linking of gelatin and PEG. Ag nanoparticles and metronidazole were incorporated into the sponges to improve their antibacterial activity. Topical gels were loaded with essential oils and Ag nanoparticles and prepared from CMC and poloxamer. The prepared sponges and topical gels were evaluated using various analysis and characterization techniques. SEM/EDX, FTIR, and TGA were employed to characterize gelatin/PEG hybrid sponges followed by porosity, in vitro biodegradability, cytotoxicity, and antibacterial studies. FTIR, SEM/EDX, and TGA confirmed their physicochemical properties and successful fabrication of sponges loaded with metronidazole and Ag nanoparticles. The sponges were biodegradable, indicating their capability to induce skin regeneration. The drug release studies showed a rapid release of metronidazole 28.32-71.97 percent from the sponges over the first hour, followed by a sustained drug release. The Ag nanoparticles were released in a sustained manner, suggesting that these sponges can rapidly destroy bacteria and inhibit persisting bacterial infections as well as protect the lesion bed from further bacteria infections. The in vitro antibacterial studies of sponges displayed superior antibacterial activity against most of the Gram-negative and Gram-positive bacteria strains commonly found in chronic wound infections with a MIC value of 15.625 μg/mL. In vitro cytotoxicity experiments revealed excellent biocompatibility with a percent cell viability of more than 70 percent. The in vitro wound scratch healing assay exhibited that the sponges encapsulated with only metronidazole promoted high cell migration than the dual drug-loaded sponges and untreated cells, suggesting its potential to quicken the wound healing process. CMC/Poloxamer topical gels were also characterized by FTIR, followed by pH, viscosity, spreadability, cytotoxicity, and antibacterial studies. FTIR showed successful preparation of CMC/Poloxamer topical gels loaded with essential oils and Ag nanoparticles. The topical gels exhibited pH in the range of 5.20-6.68, spreadability between 5.4 and 5.9 cm, and viscosity ranged from 216 to 1200 cP at 50 rpm and 210–858 cP at 100 rpm. The in vitro drug release studies demonstrated that Ag nanoparticles were released from the topical gels in a sustained manner. Most formulated topical gels demonstrated superior antimicrobial efficacy against Gram-positive and Gram-negative bacteria strains than the blank gel and controls. The cytotoxicity analysis displayed more than 90.83 percent cell viability for the topical gels, revealing excellent biocompatibility. The outcomes revealed that the topical gels enriched with essential oils lavender and tea tree and Ag nanoparticles and sponges incorporated with metronidazole and Ag nanoparticles are potential wound dressing scaffolds that can be employed for the treatment of chronic infected injuries. The in vitro wound healing experiments showed that the HaCaT cells cultured with gels co-enriched with lavender oil and Ag nanoparticles possessed a higher rate of closure in comparison to the untreated cells for 96 hours. , Thesis (PhD) -- Faculty of Science and Agriculture, 2022
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Synthesis and in vitro biological studies of ursolic acid-based hybrid compounds
- Authors: Khwaza, Vuyolwethu
- Date: 2022
- Subjects: Herbal medicine , Herbs -- Therapeutic use , Antineoplastic antibiotics
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/27797 , vital:69689
- Description: Ursolic acid UA, a pentacyclic triterpenoid that is commonly found in many medicinal herbs and fruits, has been identified as a potential source of therapeutic agents because of its potent biological effects, which include its potential anticancer and antimicrobial activities. However, its limited solubility, rapid metabolism and poor bioavailability inhibit its clinical applications. Numerous UA derivatives have been prepared over the past years in an effort to mitigate the drawbacks associated with UA, as new chemical entities for the treatment of various infections. There is very little progress in the discovery of efficient UA derivatives. In this study, a class of ester and amide-linked ursolic acid-based hybrid compounds fused with selected pharmaceutical scaffolds were successfully synthesized using amidation and esterification reactions and tested for antibacterial and cytotoxicity activities. Hybridizing UA with other known pharmaceutical scaffolds has the potential of overcoming its drawbacks. FT-IR, Mass Spectroscopy, and 1H13C-NMR spectroscopy were used to confirm the structures of the synthesized hybrid compounds. Among the tested ester-linked hybrid compounds in Chapter three, compounds 3.14-3.19,3.21, 3.34, 3.31, and 3.30 demonstrated significant antibacterial activities against some tested bacteria, with MIC values of 15.625 μgml. Furthermore, the in vitro cytotoxicity of these hybrids was determined using the MTT assay against three human tumor cell lines MCF7, MDA-MB-231, and HeLa cells. Compounds 3.19 and 3.34 were found to have better cytotoxic activity when compared to ursolic acid, with IC50 values of 46.99 and 48.18 μg ml respectively. Both compounds revealed more promising docking results, presenting favourable binding interactions as well as better docking energy against the MCF 7 protein target compared to the parent compound ursolic acid. In Chapter Four, among the tested amide-linked hybrid compounds, Compounds 4.17 and 4.24 demonstrated significant antibacterial activity against the majority of bacterial strains with MIC values of 15.625 gml. Compound 4.24 exhibited a MIC value of 15.625gmL against BS, SA, PV, KO, PM, and EC. Compound 4.23 was more cytotoxic to HeLa cells than ursolic acid. Furthermore, molecular docking calculations revealed that compound 4.16 strongly binds to the protein epidermal growth factor receptor while e compounds 4.17 and 4.24 showed a strong binding affinity for the methionyl-tRNA synthetase. In both cases, the hybrid compounds showed better conformational fittings in the active site of the targeted proteins as compared to the parent ursolic acid. , Thesis (MSc) -- Faculty of Science and Agriculture, 2022
- Full Text:
- Authors: Khwaza, Vuyolwethu
- Date: 2022
- Subjects: Herbal medicine , Herbs -- Therapeutic use , Antineoplastic antibiotics
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/27797 , vital:69689
- Description: Ursolic acid UA, a pentacyclic triterpenoid that is commonly found in many medicinal herbs and fruits, has been identified as a potential source of therapeutic agents because of its potent biological effects, which include its potential anticancer and antimicrobial activities. However, its limited solubility, rapid metabolism and poor bioavailability inhibit its clinical applications. Numerous UA derivatives have been prepared over the past years in an effort to mitigate the drawbacks associated with UA, as new chemical entities for the treatment of various infections. There is very little progress in the discovery of efficient UA derivatives. In this study, a class of ester and amide-linked ursolic acid-based hybrid compounds fused with selected pharmaceutical scaffolds were successfully synthesized using amidation and esterification reactions and tested for antibacterial and cytotoxicity activities. Hybridizing UA with other known pharmaceutical scaffolds has the potential of overcoming its drawbacks. FT-IR, Mass Spectroscopy, and 1H13C-NMR spectroscopy were used to confirm the structures of the synthesized hybrid compounds. Among the tested ester-linked hybrid compounds in Chapter three, compounds 3.14-3.19,3.21, 3.34, 3.31, and 3.30 demonstrated significant antibacterial activities against some tested bacteria, with MIC values of 15.625 μgml. Furthermore, the in vitro cytotoxicity of these hybrids was determined using the MTT assay against three human tumor cell lines MCF7, MDA-MB-231, and HeLa cells. Compounds 3.19 and 3.34 were found to have better cytotoxic activity when compared to ursolic acid, with IC50 values of 46.99 and 48.18 μg ml respectively. Both compounds revealed more promising docking results, presenting favourable binding interactions as well as better docking energy against the MCF 7 protein target compared to the parent compound ursolic acid. In Chapter Four, among the tested amide-linked hybrid compounds, Compounds 4.17 and 4.24 demonstrated significant antibacterial activity against the majority of bacterial strains with MIC values of 15.625 gml. Compound 4.24 exhibited a MIC value of 15.625gmL against BS, SA, PV, KO, PM, and EC. Compound 4.23 was more cytotoxic to HeLa cells than ursolic acid. Furthermore, molecular docking calculations revealed that compound 4.16 strongly binds to the protein epidermal growth factor receptor while e compounds 4.17 and 4.24 showed a strong binding affinity for the methionyl-tRNA synthetase. In both cases, the hybrid compounds showed better conformational fittings in the active site of the targeted proteins as compared to the parent ursolic acid. , Thesis (MSc) -- Faculty of Science and Agriculture, 2022
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The nexus between petroleum energy security and South Africa’s foreign policy
- Authors: Seshibedi S S
- Date: 2022
- Subjects: Petroleum industry and trade -- Government policy , Energy industries , Petroleum law and legislation
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/27165 , vital:66325
- Description: This study sets out to explore the nexus between South Africa’s petroleum energy security and its foreign policy. The specific objectives of the research include: to find out the state of South Africa’s oil energy security, the extent to which oil is a consideration in the development of the country’s foreign policy and the extent to which its foreign policy is framed to favour oil exporting states. Qualitative research methodology is applied in this study with the Realism as the theoretical framework. The study found out that the state of South Africa’s oil energy security, which compels the country to rely on foreign oil, provides grounds for foreign policy intervention, with discernible espousal of relations with oil exporting states that is different to those that do not produce oil. The study also found that oil is a consideration in the development of South Africa’s foreign policy, as the country’s policy strategy towards oil exporting states is to build relations of bilateral nature, through diplomatic instruments, among others. South Africa’s regional politics makes discernible the fact that South Africa prefers a policy of multilateralism to manage its relations with SADC and SACU members who are not oil exporting, while evidence showed that bilateral relations are preferred towards oil exporting states. The study recommends amongst others, that South Africa’s over-reliance on foreign oil can be mitigated by taking the lead in transitioning to renewable energy. , Thesis (MSoc) -- Faculty of Social Sciences and Humanities, 2022
- Full Text:
- Authors: Seshibedi S S
- Date: 2022
- Subjects: Petroleum industry and trade -- Government policy , Energy industries , Petroleum law and legislation
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/27165 , vital:66325
- Description: This study sets out to explore the nexus between South Africa’s petroleum energy security and its foreign policy. The specific objectives of the research include: to find out the state of South Africa’s oil energy security, the extent to which oil is a consideration in the development of the country’s foreign policy and the extent to which its foreign policy is framed to favour oil exporting states. Qualitative research methodology is applied in this study with the Realism as the theoretical framework. The study found out that the state of South Africa’s oil energy security, which compels the country to rely on foreign oil, provides grounds for foreign policy intervention, with discernible espousal of relations with oil exporting states that is different to those that do not produce oil. The study also found that oil is a consideration in the development of South Africa’s foreign policy, as the country’s policy strategy towards oil exporting states is to build relations of bilateral nature, through diplomatic instruments, among others. South Africa’s regional politics makes discernible the fact that South Africa prefers a policy of multilateralism to manage its relations with SADC and SACU members who are not oil exporting, while evidence showed that bilateral relations are preferred towards oil exporting states. The study recommends amongst others, that South Africa’s over-reliance on foreign oil can be mitigated by taking the lead in transitioning to renewable energy. , Thesis (MSoc) -- Faculty of Social Sciences and Humanities, 2022
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