Genetic diversity, evolutionary relationships and conservation of southern African Labeo fishes in relation to water management
- Authors: Ramoejane, Mpho
- Date: 2017
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/5209 , vital:20786
- Description: Labeo spp. are large, herbivorous fishes that are important components of aquatic ecosystems and are a high conservation priority in South Africa. This thesis contributes to determination of conservation priorities for Labeo umbratus (Smith 1841) by resolving the taxonomic status of this species in the evolutionary context of southern African Labeo spp., assessing the presence of unique lineages in historically isolated river basins, and assessing the threat of intra- and interspecific hybridisation associated with introductions. Phylogenetic analyses of five DNA sequence data sets (cytochrome c oxidase subunit I gene [COI], cytochrome b gene [Cyt b], Recombination activating gene 1 [Rag1], COI+Rag1 and COI+Cyt b+Rag1) showed that the Labeo umbratus group (sensu Reid, 1985), which comprises the species Labeo umbratus, Labeo capensis (Smith 1841), Labeo seeberi Gilchrist and Thompson 1911 and Labeo rubromaculatus Gilchrist and Thompson 1913, is monophyletic, morphologically distinct and geographically disjunct from other African Labeo spp. groups except in the Tugela River system were L. rubromaculatus co-occurs with Labeo molybdinus Du Plessis 1963. Phylogeographic analysis of mitochondrial DNA (Cyt b) sequence data demonstrated that the populations of the L. umbratus from the Orange and the southward-flowing river systems are reciprocally monophyletic and were identified as evolutionary significant units. The populations in the southward-flowing river systems were further divided into southwestern (Gourits and Gamtoos) and southeastern (Sundays, Bushmans, Great Fish, Keiskamma, Buffalo and Nahoon) polyp hyletic sublineages. Four management units (Gourits Basin; Gamtoos Basin; Sundays+Bushmans+Great Fish River Basins; and Keiskamma+Buffalo+Nahoon River Basin) were not reciprocally monophyletic but were proposed on the basis of containing unique haplotype frequencies for conservation purposes. To evaluate the threat of hybridisation to the genetic integrity of L. umbratus, the occurrence of Labeo umbratus x L. capensis hybrids was investigated using mtDNA Cyt b and nDNA S7 intron sequence data and morphological data. Genetic evidence for interspecific hybridisation was detected for populations in two impoundments, Hardap Dam (Orange River Basin) and Darlington Dam (Sundays River Basin, Eastern Cape, South Africa). Some putative hybrids were identifiable morphologically on account of intermediacy between the parental species in meristic and morphometric characters. Translocation via direct stocking (Hardap Dam) or via an inter-basin water transfer scheme (Darlington Dam) was identified as a driver for hybridisation. Introductions associated with an inter-basin water transfer scheme has resulted in introgression between the previously isolated Orange River and southern lineages of L. umbratus. Further translocation of fish from these affected areas to non-contaminated river systems and impoundments such as Kat River (Great Fish River) and Slagboom (Sundays River) should be avoided.
- Full Text:
- Date Issued: 2017
- Authors: Ramoejane, Mpho
- Date: 2017
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/5209 , vital:20786
- Description: Labeo spp. are large, herbivorous fishes that are important components of aquatic ecosystems and are a high conservation priority in South Africa. This thesis contributes to determination of conservation priorities for Labeo umbratus (Smith 1841) by resolving the taxonomic status of this species in the evolutionary context of southern African Labeo spp., assessing the presence of unique lineages in historically isolated river basins, and assessing the threat of intra- and interspecific hybridisation associated with introductions. Phylogenetic analyses of five DNA sequence data sets (cytochrome c oxidase subunit I gene [COI], cytochrome b gene [Cyt b], Recombination activating gene 1 [Rag1], COI+Rag1 and COI+Cyt b+Rag1) showed that the Labeo umbratus group (sensu Reid, 1985), which comprises the species Labeo umbratus, Labeo capensis (Smith 1841), Labeo seeberi Gilchrist and Thompson 1911 and Labeo rubromaculatus Gilchrist and Thompson 1913, is monophyletic, morphologically distinct and geographically disjunct from other African Labeo spp. groups except in the Tugela River system were L. rubromaculatus co-occurs with Labeo molybdinus Du Plessis 1963. Phylogeographic analysis of mitochondrial DNA (Cyt b) sequence data demonstrated that the populations of the L. umbratus from the Orange and the southward-flowing river systems are reciprocally monophyletic and were identified as evolutionary significant units. The populations in the southward-flowing river systems were further divided into southwestern (Gourits and Gamtoos) and southeastern (Sundays, Bushmans, Great Fish, Keiskamma, Buffalo and Nahoon) polyp hyletic sublineages. Four management units (Gourits Basin; Gamtoos Basin; Sundays+Bushmans+Great Fish River Basins; and Keiskamma+Buffalo+Nahoon River Basin) were not reciprocally monophyletic but were proposed on the basis of containing unique haplotype frequencies for conservation purposes. To evaluate the threat of hybridisation to the genetic integrity of L. umbratus, the occurrence of Labeo umbratus x L. capensis hybrids was investigated using mtDNA Cyt b and nDNA S7 intron sequence data and morphological data. Genetic evidence for interspecific hybridisation was detected for populations in two impoundments, Hardap Dam (Orange River Basin) and Darlington Dam (Sundays River Basin, Eastern Cape, South Africa). Some putative hybrids were identifiable morphologically on account of intermediacy between the parental species in meristic and morphometric characters. Translocation via direct stocking (Hardap Dam) or via an inter-basin water transfer scheme (Darlington Dam) was identified as a driver for hybridisation. Introductions associated with an inter-basin water transfer scheme has resulted in introgression between the previously isolated Orange River and southern lineages of L. umbratus. Further translocation of fish from these affected areas to non-contaminated river systems and impoundments such as Kat River (Great Fish River) and Slagboom (Sundays River) should be avoided.
- Full Text:
- Date Issued: 2017
The genetic integrity of Labeo capensis and L. umbratus (Cyprinidae) in South Africa in relation to inter-basin water transfer schemes
- Authors: Ramoejane, Mpho
- Date: 2011
- Subjects: Cyprinidae -- South Africa -- Darlington Dam , Cyprinidae -- Namibia -- Hardap Dam , Labeo -- South Africa -- Darlington Dam , Labeo -- Namibia -- Hardap Dam , Fishes -- Hybridisation -- South Africa -- Darlington Dam , Fishes -- Hybridisation -- Namibia -- Hardap Dam , Fishes -- Anatomy -- South Africa -- Darlington Dam , Fishes -- Anatomy -- Namibia -- Hardap Dam , Water transfer -- South Africa-- Darlington Dam , Water transfer -- Namibia -- Hardap Dam
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5367 , http://hdl.handle.net/10962/d1013049
- Description: The Orange-Fish and Cookhouse tunnels that are part of a major inter-basin water transfer scheme (IBT) act as a pathway for several fish species from the Orange River system to enter the Great Fish and Sundays River systems in South Africa. These include Labeo capensis and L. umbratus. Labeo capensis was restricted to the Orange River system before the inter-basin water transfer scheme. Labeo umbratus occurred naturally in the Orange River and in southern flowing river systems. Previous studies showed that the two species hybridise in Hardap Dam, located in a tributary of the Orange River system in Namibia. There are also unconfirmed reports of hybrids from Darlington Dam on the Sundays River system. The aim of the thesis was to confirm hybridisation in Hardap Dam, assess whether hybridisation between L. capensis and L. umbratus has occurred in Darlington Dam and to gain a better understanding of the diversity of these two species. Morphology (morphometrics and meristics), a nuclear S7 intron and the mitochondrial cytochrome ♭ gene were used to assess for hybridisation. A total of 275 specimens were analysed from across the geographical range of the two species. The two species could be distinguished using morphometrics (dorsal fin base, interorbital width and operculum to eye distance) and meristics (lateral line, origin of the dorsal fin to lateral line, origin of the pelvic fin to lateral line and caudal peduncle scale counts) characters. Hybrids from Hardap and Darlington dams were placed between the two species clusters. Labeo umbratus from the Orange River and southern flowing rivers formed a single cluster. The two species could also be distinguished from each other with six nuclear DNA mutations and hybrids were heterozygous at such sites in both dams. Labeo umbratus populations from the Orange River and southern flowing rivers (Gouritz, Gamtoos, Sundays, Bushmans, Great Fish and Nahoon) formed a single lineage. Analysis of mitochondrial DNA, however, revealed that L. umbratus populations from the Orange River and southern flowing rivers were two lineages that differ from each other by 5 mutations. Labeo capensis could be differentiated from both these lineages. Being maternally inherited, mitochondrial DNA did not reveal hybridisation, but ten specimens with L. capensis haplotypes were found in the Darlington Dam. In Hardap Dam, however, it appears that only L. capensis mitochondrial DNA haplotypes persist, despite morphological and nuclear DNA analysis suggesting that both morphs and hybrids of the two species occur. The genetic integrity of these Labeo species has therefore been compromised in at least Hardap and Darlington dams. The Great Fish and Sundays populations are considered to be under threat of complete introgression. The Kat River and Slagboom Dam populations that were isolated before the IBTs have to remain isolated to protect the genetic integrity of the southern lineage of L. umbratus in these two systems.
- Full Text:
- Date Issued: 2011
- Authors: Ramoejane, Mpho
- Date: 2011
- Subjects: Cyprinidae -- South Africa -- Darlington Dam , Cyprinidae -- Namibia -- Hardap Dam , Labeo -- South Africa -- Darlington Dam , Labeo -- Namibia -- Hardap Dam , Fishes -- Hybridisation -- South Africa -- Darlington Dam , Fishes -- Hybridisation -- Namibia -- Hardap Dam , Fishes -- Anatomy -- South Africa -- Darlington Dam , Fishes -- Anatomy -- Namibia -- Hardap Dam , Water transfer -- South Africa-- Darlington Dam , Water transfer -- Namibia -- Hardap Dam
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5367 , http://hdl.handle.net/10962/d1013049
- Description: The Orange-Fish and Cookhouse tunnels that are part of a major inter-basin water transfer scheme (IBT) act as a pathway for several fish species from the Orange River system to enter the Great Fish and Sundays River systems in South Africa. These include Labeo capensis and L. umbratus. Labeo capensis was restricted to the Orange River system before the inter-basin water transfer scheme. Labeo umbratus occurred naturally in the Orange River and in southern flowing river systems. Previous studies showed that the two species hybridise in Hardap Dam, located in a tributary of the Orange River system in Namibia. There are also unconfirmed reports of hybrids from Darlington Dam on the Sundays River system. The aim of the thesis was to confirm hybridisation in Hardap Dam, assess whether hybridisation between L. capensis and L. umbratus has occurred in Darlington Dam and to gain a better understanding of the diversity of these two species. Morphology (morphometrics and meristics), a nuclear S7 intron and the mitochondrial cytochrome ♭ gene were used to assess for hybridisation. A total of 275 specimens were analysed from across the geographical range of the two species. The two species could be distinguished using morphometrics (dorsal fin base, interorbital width and operculum to eye distance) and meristics (lateral line, origin of the dorsal fin to lateral line, origin of the pelvic fin to lateral line and caudal peduncle scale counts) characters. Hybrids from Hardap and Darlington dams were placed between the two species clusters. Labeo umbratus from the Orange River and southern flowing rivers formed a single cluster. The two species could also be distinguished from each other with six nuclear DNA mutations and hybrids were heterozygous at such sites in both dams. Labeo umbratus populations from the Orange River and southern flowing rivers (Gouritz, Gamtoos, Sundays, Bushmans, Great Fish and Nahoon) formed a single lineage. Analysis of mitochondrial DNA, however, revealed that L. umbratus populations from the Orange River and southern flowing rivers were two lineages that differ from each other by 5 mutations. Labeo capensis could be differentiated from both these lineages. Being maternally inherited, mitochondrial DNA did not reveal hybridisation, but ten specimens with L. capensis haplotypes were found in the Darlington Dam. In Hardap Dam, however, it appears that only L. capensis mitochondrial DNA haplotypes persist, despite morphological and nuclear DNA analysis suggesting that both morphs and hybrids of the two species occur. The genetic integrity of these Labeo species has therefore been compromised in at least Hardap and Darlington dams. The Great Fish and Sundays populations are considered to be under threat of complete introgression. The Kat River and Slagboom Dam populations that were isolated before the IBTs have to remain isolated to protect the genetic integrity of the southern lineage of L. umbratus in these two systems.
- Full Text:
- Date Issued: 2011
- «
- ‹
- 1
- ›
- »