Histopathological changes in male wistar rats maintained on a water-based sutherlandia frutescens extract
- Authors: Wickens, Nicolas John
- Date: 2012
- Subjects: Rats -- Physiology , Rats as laboratory animals
- Language: English
- Type: Thesis , Doctoral , DTech
- Identifier: http://hdl.handle.net/10948/4742 , vital:20670
- Description: In this study a standardized 46 week chronic drinking water toxicity protocol was used to elucidate the toxic potential of Sutherlandia frutescens (S. frutescens) using histopathologic, morphometric and transmission electron microscopic analysis. The histopathologic changes in the duodenum, heart, kidney, liver, lung, pancreas and spleen of male Wistar rats were evaluated. Fifty-four rats were randomly divided into four groups: Group 1 – Normal diet control (ND control), n=7, Group 2 – Normal diet + plant extract (ND + p), n=9, Group 3 – High fat diet control (HFD control), n=19Group 4 – High fat diet + p (HFD + p), n=19In the high fat group male Wistar rats were fed ±55 g/day of a specialised high fat diet over a 46 week period to induce obesity and an insulin resistant state. The treatment groups (groups 2 and 4) received a dose concentration of a tea extract of the S. frutescens plant in their drinking water daily. This study showed that the consumption of S. frutescens significantly reduces weight gain in male Wistar rats on a chronic high fat diet (p≤0.001 vs. HFD control group). S. frutescens appears to propagate periportal and centrilobular glycogen storage in rat hepatocytes in the experimental groups as exemplified by a significantly (p≤0.0001 vs. control groups) increased incidences of Periodic Acid Schiff (PAS) positive staining S. frutescens also reduced intracellular lipid accumulation as made evident by the significantly lower incidence of epicardial adipose tissue (EAT), hepatic steatosis and pancreatic interstitial fat. Obesity was associated with increased fibrotic lesions such as myocardial perivascular fibrosis, centrilobular hepatic fibrosis and pancreatic periductal fibrosis. Obesity associated hypertension contributed to the widespread and significant increase in the average lesion severity of arterial congestion in all organs in the HFD control group. Pulmonary infection was equally prevalent in all rats. Despite the complex histopathology in all groups, differences in the control groups, such as, the presence of a conservative polymorphonuclear leukocyte (PMNL) infiltration, substantial intra-alveolar oedema and focal arterial wall hypertrophy in the control groups was highly suggestive of Sendai viral infection. However histopathologic evidence, in the treatment groups, suggested chronic recurrent viral infection with superimposed Mycoplasma pulmonis (M. pulmonis) bacterial infection. The impact of advanced suppurative pulmonary infection was widespread and exemplified by increased lesion incidences of spontaneous murine progressive cardiomyopathy (MCP) and spontaneous chronic progressive nephropathy (CPN) among others. In conclusion S. frutescens administered for 46 weeks to male Wistar rats significantly lowered intracellular lipid accumulation and obesity associated myocardial, renal, hepatobiliary, pulmonary and pancreatic histopathology. Moreover, duodenal, cardiovascular, hepatobiliary, pulmonary, renal, pancreatic and splenic tissue did not show histopathologic evidence of direct plant extract associated toxicity or carcinogenicity.
- Full Text:
- Date Issued: 2012
- Authors: Wickens, Nicolas John
- Date: 2012
- Subjects: Rats -- Physiology , Rats as laboratory animals
- Language: English
- Type: Thesis , Doctoral , DTech
- Identifier: http://hdl.handle.net/10948/4742 , vital:20670
- Description: In this study a standardized 46 week chronic drinking water toxicity protocol was used to elucidate the toxic potential of Sutherlandia frutescens (S. frutescens) using histopathologic, morphometric and transmission electron microscopic analysis. The histopathologic changes in the duodenum, heart, kidney, liver, lung, pancreas and spleen of male Wistar rats were evaluated. Fifty-four rats were randomly divided into four groups: Group 1 – Normal diet control (ND control), n=7, Group 2 – Normal diet + plant extract (ND + p), n=9, Group 3 – High fat diet control (HFD control), n=19Group 4 – High fat diet + p (HFD + p), n=19In the high fat group male Wistar rats were fed ±55 g/day of a specialised high fat diet over a 46 week period to induce obesity and an insulin resistant state. The treatment groups (groups 2 and 4) received a dose concentration of a tea extract of the S. frutescens plant in their drinking water daily. This study showed that the consumption of S. frutescens significantly reduces weight gain in male Wistar rats on a chronic high fat diet (p≤0.001 vs. HFD control group). S. frutescens appears to propagate periportal and centrilobular glycogen storage in rat hepatocytes in the experimental groups as exemplified by a significantly (p≤0.0001 vs. control groups) increased incidences of Periodic Acid Schiff (PAS) positive staining S. frutescens also reduced intracellular lipid accumulation as made evident by the significantly lower incidence of epicardial adipose tissue (EAT), hepatic steatosis and pancreatic interstitial fat. Obesity was associated with increased fibrotic lesions such as myocardial perivascular fibrosis, centrilobular hepatic fibrosis and pancreatic periductal fibrosis. Obesity associated hypertension contributed to the widespread and significant increase in the average lesion severity of arterial congestion in all organs in the HFD control group. Pulmonary infection was equally prevalent in all rats. Despite the complex histopathology in all groups, differences in the control groups, such as, the presence of a conservative polymorphonuclear leukocyte (PMNL) infiltration, substantial intra-alveolar oedema and focal arterial wall hypertrophy in the control groups was highly suggestive of Sendai viral infection. However histopathologic evidence, in the treatment groups, suggested chronic recurrent viral infection with superimposed Mycoplasma pulmonis (M. pulmonis) bacterial infection. The impact of advanced suppurative pulmonary infection was widespread and exemplified by increased lesion incidences of spontaneous murine progressive cardiomyopathy (MCP) and spontaneous chronic progressive nephropathy (CPN) among others. In conclusion S. frutescens administered for 46 weeks to male Wistar rats significantly lowered intracellular lipid accumulation and obesity associated myocardial, renal, hepatobiliary, pulmonary and pancreatic histopathology. Moreover, duodenal, cardiovascular, hepatobiliary, pulmonary, renal, pancreatic and splenic tissue did not show histopathologic evidence of direct plant extract associated toxicity or carcinogenicity.
- Full Text:
- Date Issued: 2012
An epidemiological study of childhood asthma care by general practitioners in the Nelson Mandela Metropole
- Authors: Wickens, Nicolas John
- Date: 2003
- Subjects: Asthma in children -- South Africa -- Nelson Mandela Metropole , Epidemiology -- South Africa -- Nelson Mandela Metropole , Physicians (General practice) -- South Africa -- Nelson Mandela Metropole
- Language: English
- Type: Thesis , Masters , MTech (Biomedical Technology)
- Identifier: vital:10825 , http://hdl.handle.net/10948/124 , Asthma in children -- South Africa -- Nelson Mandela Metropole , Epidemiology -- South Africa -- Nelson Mandela Metropole , Physicians (General practice) -- South Africa -- Nelson Mandela Metropole
- Description: The purpose of this study was to measure and evaluate GPs' practices, perceptions, and beliefs with regard to chronic childhood asthma management and to identify GPs' attitudes towards practice guidelines in particular the SACAWG (South African Childhood Asthma Work Group) guideline (Motala et al., 2000). In 2001 a single period cross-sectional survey was conducted among 300 GPs in the Nelson Mandela Metropole (NMM) which comprised the Port Elizabeth, Uitenhage, and Despatch regions. GPs in clinical practice in the NMM were identified from the register of the Health Professions Council (HPC) CD-ROM (2000). The register contains names and professional information on all physicians in South Africa who have completed or in the process of completing requirements to practice medicine. GPs meeting the following criteria were identified: (1) primary self-designated practice specialty of general practice; (2) practice location in Port Elizabeth, Uitenhage, and Despatch areas; and (3) engaged in direct patient care. The HPC register contained listings for 300 GPs that matched the study criteria. Data were collected by means of a structured self-administered questionnaire. Questionnaires with a return envelope were mailed to the GPs' practices. Questionnaires were returned by 89 of the 300 eligible NMM GPs, reflecting a 29,7 % response rate. A 43-item Chronic Childhood Asthma Management questionnaire was developed and validated to assess the delivery of asthma care by GPs in the NMM. Forty-four per cent of the respondents reported performing spirometry on patients with newly diagnosed asthma as part of their initial evaluation. For patients with moderate persistent asthma prescribing of inhaled corticosteroids (ICSs) varied by patient age, with 57,4% of physicians routinely prescribing them for patients < 5 years, compared with 76,5% of physicians prescribing them for patients 5 years. Awareness of the SACAWG guidelines among these GPs was high, with 76,8% reporting that they have heard of the guidelines, and 59,4% reporting having read them. 14,5 per cent of the GPs reported developing written treatment plans for patients with moderate or severe asthma. To achieve the best results with their asthma patients, 68,1% of the physicians reported that patient compliance was imperative, 49,3% reported education as the most important, pharmacotherapy / good products was reported as most important by 29,0% of the group, environmental control was cited by 18,8% of the GPs. Several aspects of the SACAWG guidelines appear to have been incorporated into clinical practice by GPs in the NMM, whereas other recommendations do not appear to have been readily adopted. This information suggests areas for intervention to improve primary care for asthma in the NMM.
- Full Text:
- Date Issued: 2003
- Authors: Wickens, Nicolas John
- Date: 2003
- Subjects: Asthma in children -- South Africa -- Nelson Mandela Metropole , Epidemiology -- South Africa -- Nelson Mandela Metropole , Physicians (General practice) -- South Africa -- Nelson Mandela Metropole
- Language: English
- Type: Thesis , Masters , MTech (Biomedical Technology)
- Identifier: vital:10825 , http://hdl.handle.net/10948/124 , Asthma in children -- South Africa -- Nelson Mandela Metropole , Epidemiology -- South Africa -- Nelson Mandela Metropole , Physicians (General practice) -- South Africa -- Nelson Mandela Metropole
- Description: The purpose of this study was to measure and evaluate GPs' practices, perceptions, and beliefs with regard to chronic childhood asthma management and to identify GPs' attitudes towards practice guidelines in particular the SACAWG (South African Childhood Asthma Work Group) guideline (Motala et al., 2000). In 2001 a single period cross-sectional survey was conducted among 300 GPs in the Nelson Mandela Metropole (NMM) which comprised the Port Elizabeth, Uitenhage, and Despatch regions. GPs in clinical practice in the NMM were identified from the register of the Health Professions Council (HPC) CD-ROM (2000). The register contains names and professional information on all physicians in South Africa who have completed or in the process of completing requirements to practice medicine. GPs meeting the following criteria were identified: (1) primary self-designated practice specialty of general practice; (2) practice location in Port Elizabeth, Uitenhage, and Despatch areas; and (3) engaged in direct patient care. The HPC register contained listings for 300 GPs that matched the study criteria. Data were collected by means of a structured self-administered questionnaire. Questionnaires with a return envelope were mailed to the GPs' practices. Questionnaires were returned by 89 of the 300 eligible NMM GPs, reflecting a 29,7 % response rate. A 43-item Chronic Childhood Asthma Management questionnaire was developed and validated to assess the delivery of asthma care by GPs in the NMM. Forty-four per cent of the respondents reported performing spirometry on patients with newly diagnosed asthma as part of their initial evaluation. For patients with moderate persistent asthma prescribing of inhaled corticosteroids (ICSs) varied by patient age, with 57,4% of physicians routinely prescribing them for patients < 5 years, compared with 76,5% of physicians prescribing them for patients 5 years. Awareness of the SACAWG guidelines among these GPs was high, with 76,8% reporting that they have heard of the guidelines, and 59,4% reporting having read them. 14,5 per cent of the GPs reported developing written treatment plans for patients with moderate or severe asthma. To achieve the best results with their asthma patients, 68,1% of the physicians reported that patient compliance was imperative, 49,3% reported education as the most important, pharmacotherapy / good products was reported as most important by 29,0% of the group, environmental control was cited by 18,8% of the GPs. Several aspects of the SACAWG guidelines appear to have been incorporated into clinical practice by GPs in the NMM, whereas other recommendations do not appear to have been readily adopted. This information suggests areas for intervention to improve primary care for asthma in the NMM.
- Full Text:
- Date Issued: 2003
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