Effect of pre-slaughter stress of pigs on the levels of cortisol, creatine kinase and their subsequent relationship with pork quality
- Authors: Nzolo, Jama
- Date: 2014
- Subjects: Slaughtering and slaughter-houses , Pork -- Quality , Swine -- Handling , Livestock -- Handling , Creatine kinase
- Language: English
- Type: Thesis , Masters , MSc Agric (Animal Science)
- Identifier: vital:11837 , http://hdl.handle.net/10353/d1021287 , Slaughtering and slaughter-houses , Pork -- Quality , Swine -- Handling , Livestock -- Handling , Creatine kinase
- Description: The study was conducted to determine the effect of pre-slaughter stress of pigs on the levels of cortisol, creatine kinase (CK) and their subsequent relationship with pork quality. From our knowledge, there is paucity of information on transportation, lairage duration and sex effects on saliva cortisol and its relation with serum cortisol, urine cortisol and other meat quality traits. Stage one of the study assessed the effect of sex and time to slaughter (transport and lairage duration) on the levels of cortisol and CK in crossbred pigs. The second stage of the study evaluated the effect of sex and lairage duration on pork quality. The trials comprised of 60 22-weeks old commercial crossbred pigs [(30 boars vs. 30 gilts) with an average body weight of 76.9kg for gilts and 78.3kg for boars. Thirty pigs (15 Boars vs. 15 Gilts) were slaughtered on arrival after a 120km road journey and the other 30, after travelling the same road and distance, were slaughtered after having been in the lairage for 20 hours. Saliva samples were obtained during three time periods: between 07:00-10:00 on the day before the journey, on arrival at the abattoir and after lairage. Levels of cortisol in serum and urine and CK in serum samples collected at slaughter were determined. The samples (saliva and serum) were centrifuged at 20oC for 10 min at 3550 x g and stored at -20oC in separate vials until analysis. Pork quality was assessed using muscle pH, colour, thawing and cooking losses and Warner-Bratzler Shear Force. Correlations between cortisol levels in saliva, serum and urine and meat quality were assessed. The effect of sex and time to slaughter on saliva cortisol was significant. Statistical analysis showed significant interaction of sex by time to slaughter on serum cortisol. It was only the effect of sex that demonstrated higher (P <0.05) serum creatine kinase levels in gilts. Likewise, urine cortisol was influenced (P <0.05) only by time to slaughter. Saliva cortisol after transportation (r = 0.52) was correlated (P <0.05) with urine cortisol. The study suggests that there are greater responses of the Hypothalamic-pituitary-adrenal axis following time to slaughter (transport and lairage duration) between gilts and boars. This can be used in measuring cortisol in saliva, serum and urine, and serum creatine kinase to determine stress levels in pigs. Besides the lightness (L*) colour value which was significantly lower (P <0.05) in meat from gilts than from boars, the results of this study showed no interaction or individual effect (sex or lairage duration) on pH and other colour measurements. Meat cooking loss was affected (P <0.05) by gender x lairage interaction. Effect of lairage duration (as an individual effect) on the measured meat quality traits was not significant (P <0.05). Correlation analysis showed significant (P <0.05) negative relation between baseline saliva cortisol (r = -0.40) and the pork L* value. Saliva cortisol after lairage duration (r = -0.38 and r = 0.38) was correlated (P <0.05) with pH45 and pork a* value, respectively. Serum cortisol (r = -0.35) was negatively correlated (P <0.05) with pH45. Moreover, there were negative correlations (P <0.05) between saliva cortisol after transportation (r = -0.35), saliva cortisol after lairage duration (r = -0.44), serum cortisol (r = -0.40) and meat cooking loss. The study suggests that cortisol in saliva and serum can be used to determine effect of time to slaughter (transport and lairage duration) on meat quality traits from gilts and boars. Keywords: Boars; gilts; saliva cortisol; serum cortisol; serum creatine kinase; urine cortisol.
- Full Text:
- Date Issued: 2014
- Authors: Nzolo, Jama
- Date: 2014
- Subjects: Slaughtering and slaughter-houses , Pork -- Quality , Swine -- Handling , Livestock -- Handling , Creatine kinase
- Language: English
- Type: Thesis , Masters , MSc Agric (Animal Science)
- Identifier: vital:11837 , http://hdl.handle.net/10353/d1021287 , Slaughtering and slaughter-houses , Pork -- Quality , Swine -- Handling , Livestock -- Handling , Creatine kinase
- Description: The study was conducted to determine the effect of pre-slaughter stress of pigs on the levels of cortisol, creatine kinase (CK) and their subsequent relationship with pork quality. From our knowledge, there is paucity of information on transportation, lairage duration and sex effects on saliva cortisol and its relation with serum cortisol, urine cortisol and other meat quality traits. Stage one of the study assessed the effect of sex and time to slaughter (transport and lairage duration) on the levels of cortisol and CK in crossbred pigs. The second stage of the study evaluated the effect of sex and lairage duration on pork quality. The trials comprised of 60 22-weeks old commercial crossbred pigs [(30 boars vs. 30 gilts) with an average body weight of 76.9kg for gilts and 78.3kg for boars. Thirty pigs (15 Boars vs. 15 Gilts) were slaughtered on arrival after a 120km road journey and the other 30, after travelling the same road and distance, were slaughtered after having been in the lairage for 20 hours. Saliva samples were obtained during three time periods: between 07:00-10:00 on the day before the journey, on arrival at the abattoir and after lairage. Levels of cortisol in serum and urine and CK in serum samples collected at slaughter were determined. The samples (saliva and serum) were centrifuged at 20oC for 10 min at 3550 x g and stored at -20oC in separate vials until analysis. Pork quality was assessed using muscle pH, colour, thawing and cooking losses and Warner-Bratzler Shear Force. Correlations between cortisol levels in saliva, serum and urine and meat quality were assessed. The effect of sex and time to slaughter on saliva cortisol was significant. Statistical analysis showed significant interaction of sex by time to slaughter on serum cortisol. It was only the effect of sex that demonstrated higher (P <0.05) serum creatine kinase levels in gilts. Likewise, urine cortisol was influenced (P <0.05) only by time to slaughter. Saliva cortisol after transportation (r = 0.52) was correlated (P <0.05) with urine cortisol. The study suggests that there are greater responses of the Hypothalamic-pituitary-adrenal axis following time to slaughter (transport and lairage duration) between gilts and boars. This can be used in measuring cortisol in saliva, serum and urine, and serum creatine kinase to determine stress levels in pigs. Besides the lightness (L*) colour value which was significantly lower (P <0.05) in meat from gilts than from boars, the results of this study showed no interaction or individual effect (sex or lairage duration) on pH and other colour measurements. Meat cooking loss was affected (P <0.05) by gender x lairage interaction. Effect of lairage duration (as an individual effect) on the measured meat quality traits was not significant (P <0.05). Correlation analysis showed significant (P <0.05) negative relation between baseline saliva cortisol (r = -0.40) and the pork L* value. Saliva cortisol after lairage duration (r = -0.38 and r = 0.38) was correlated (P <0.05) with pH45 and pork a* value, respectively. Serum cortisol (r = -0.35) was negatively correlated (P <0.05) with pH45. Moreover, there were negative correlations (P <0.05) between saliva cortisol after transportation (r = -0.35), saliva cortisol after lairage duration (r = -0.44), serum cortisol (r = -0.40) and meat cooking loss. The study suggests that cortisol in saliva and serum can be used to determine effect of time to slaughter (transport and lairage duration) on meat quality traits from gilts and boars. Keywords: Boars; gilts; saliva cortisol; serum cortisol; serum creatine kinase; urine cortisol.
- Full Text:
- Date Issued: 2014
Effects of dietary inclusion of Moringa oleifera leaf meal on growth performance, physico-chemical attributes, oxidative stability and sensory quality of pork
- Authors: Nduku, Xola Pauline
- Date: 2014
- Subjects: Pork -- Quality , Food -- Sensory evaluation , Fatty acids , Moringa , Meat -- Quality
- Language: English
- Type: Thesis , Masters , MSc Agric (Animal Science)
- Identifier: vital:11836 , http://hdl.handle.net/10353/d1021281 , Pork -- Quality , Food -- Sensory evaluation , Fatty acids , Moringa , Meat -- Quality
- Description: The objective of the study was to determine growth performance, physico-chemical attributes, oxidative stability and sensory quality of pork from pigs fed one of three dietary treatments, eac containing 0%, 2.5% or 5% (T1, T2 and T3, respectively) Moringa oleifera leaf meal (MOLM). Dietary treatments were formulated to be isonitrogenous and isoenergetic for weaner (6 – 8 weeks) and grower (9 – 13 weeks) phases. Twelve Large White (LW) and 12 Kolbroek (KB) male pigs at 6 weeks of age, initially weighing an average 10 kg, were randomly allocated to one of the dietary treatments, each with four replicates, in individual pens. Feed and water were offered ad libitum. The Longissimus thoracis et lumborum (LTL) muscle of each carcass (n = 24) was sampled for meat quality and fatty acid analyses. Breed differences in measured parameters were observed. In pigs receiving 5% MOLM, the LW had significantly the highest ADFI (P<0.05) than KB pigs. In pigs receiving 2.5% and 5% MOLM, backfat thickness was significantly highest (P<0.05) compared to those receiving 0% MOML. Dietary inclusion of MOLM had no significant effects (P>0.05) on the physico-chemical quality of pork from LW pigs; although it significantly (P<0.05) increased a* and reduced WBSF values in pork from KB pigs. Pork from LW pigs receiving 5% MOLM had significantly increased (P<0.05) n-3 content of the subcutaneous tissue of LW pigs compared to KB, and also when compread to other treatment groups. In both breeds, the composition of PUFA: SFA and n-6: n-3 in the subcutaneous adipose tissue was significantly (P<0.05) lower in T2 and T3 than in T1. The n-3 levels for pork muscle from LW pigs receiving 2.5% and 5% MOLM, and from KB pigs receiving 5% MOLM, were significantly (P<0.05) lower. Inclusions of MOLM significantly (P<0.05) reduced the n-6: n-3 fatty acids in pork from both LW pigs and KB pigs. Consumer scores on sensory attributes (aroma intensity, initial impression of juiciness, first bite, sustained impression of juiciness, muscle fibre and overall tenderness and overall flavor intensity) significantly (P<0.05) increased as the level of MOLM inclusion in the diet increased; and higher scores were observed in fried meat than in boiled for most sensory attributes. It may be concluded that inclusion of MOLM in pig diets up to 5% improved the ADFI, a*, tenderness of pork without adversely affecting the FCR and other physico-chemical quality attributes; and resulted in desired increase in levels of n-3 and reduced the n-6: n-3 fatty acid ratio.
- Full Text:
- Date Issued: 2014
- Authors: Nduku, Xola Pauline
- Date: 2014
- Subjects: Pork -- Quality , Food -- Sensory evaluation , Fatty acids , Moringa , Meat -- Quality
- Language: English
- Type: Thesis , Masters , MSc Agric (Animal Science)
- Identifier: vital:11836 , http://hdl.handle.net/10353/d1021281 , Pork -- Quality , Food -- Sensory evaluation , Fatty acids , Moringa , Meat -- Quality
- Description: The objective of the study was to determine growth performance, physico-chemical attributes, oxidative stability and sensory quality of pork from pigs fed one of three dietary treatments, eac containing 0%, 2.5% or 5% (T1, T2 and T3, respectively) Moringa oleifera leaf meal (MOLM). Dietary treatments were formulated to be isonitrogenous and isoenergetic for weaner (6 – 8 weeks) and grower (9 – 13 weeks) phases. Twelve Large White (LW) and 12 Kolbroek (KB) male pigs at 6 weeks of age, initially weighing an average 10 kg, were randomly allocated to one of the dietary treatments, each with four replicates, in individual pens. Feed and water were offered ad libitum. The Longissimus thoracis et lumborum (LTL) muscle of each carcass (n = 24) was sampled for meat quality and fatty acid analyses. Breed differences in measured parameters were observed. In pigs receiving 5% MOLM, the LW had significantly the highest ADFI (P<0.05) than KB pigs. In pigs receiving 2.5% and 5% MOLM, backfat thickness was significantly highest (P<0.05) compared to those receiving 0% MOML. Dietary inclusion of MOLM had no significant effects (P>0.05) on the physico-chemical quality of pork from LW pigs; although it significantly (P<0.05) increased a* and reduced WBSF values in pork from KB pigs. Pork from LW pigs receiving 5% MOLM had significantly increased (P<0.05) n-3 content of the subcutaneous tissue of LW pigs compared to KB, and also when compread to other treatment groups. In both breeds, the composition of PUFA: SFA and n-6: n-3 in the subcutaneous adipose tissue was significantly (P<0.05) lower in T2 and T3 than in T1. The n-3 levels for pork muscle from LW pigs receiving 2.5% and 5% MOLM, and from KB pigs receiving 5% MOLM, were significantly (P<0.05) lower. Inclusions of MOLM significantly (P<0.05) reduced the n-6: n-3 fatty acids in pork from both LW pigs and KB pigs. Consumer scores on sensory attributes (aroma intensity, initial impression of juiciness, first bite, sustained impression of juiciness, muscle fibre and overall tenderness and overall flavor intensity) significantly (P<0.05) increased as the level of MOLM inclusion in the diet increased; and higher scores were observed in fried meat than in boiled for most sensory attributes. It may be concluded that inclusion of MOLM in pig diets up to 5% improved the ADFI, a*, tenderness of pork without adversely affecting the FCR and other physico-chemical quality attributes; and resulted in desired increase in levels of n-3 and reduced the n-6: n-3 fatty acid ratio.
- Full Text:
- Date Issued: 2014
Perceptions of rural consumers on behaviour and beef quality of cattle slaughtered for traditional ceremonies in the Eastern Cape of South Africa
- Authors: Soga, Sizwe William
- Date: 2014
- Subjects: Meat -- Quality , Consumers' preferences , Consumers -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc Agric (Animal Science)
- Identifier: vital:11833 , http://hdl.handle.net/10353/d1019812 , Meat -- Quality , Consumers' preferences , Consumers -- South Africa -- Eastern Cape
- Description: The objective of the study was to determine perceptions of the consumers on how they judged behaviour and beef quality of cattle slaughtered for traditional ceremonies in the Eastern Cape Province of South Africa. A survey was conducted where a sample of 200 consumers were randomly selected and interviewed. Fifty non-descript cattle were slaughtered during traditional ceremonies and two hundred and twenty four (224) cattle were slaughtered at 3 through-put abattoirs (high, medium and low). Meat quality attributes were determined from the cattle slaughtered for traditional ceremonies and from cattle slaughtered at the abattoirs. Data for questionnaires was summarized as frequencies for each ceremony and statistical differences were analysed using chi square test (X2). The effect of Age, breed and sex on beef pH, colour, meat tenderness, temperature and cooking loss of cattle slaughtered during traditional ceremonies, at low, medium and high throughput abattoirs were analyzed using General Linear Model (PROG GLM) of SAS (2003). There was a significant age effect (P < 0.05) on beef tenderness and cooking loss of cattle slaughtered for traditional ceremonies. Age of cattle that have milk teeth to 1 year and age of 6 years to 8 years had an effect on the beef tenderness (25.55 ± 8.05) and cooking loss (48.00 ± 4.26) of cattle slaughtered for traditional ceremonies in Table 4.1. There was no significant sex effect on beef tenderness and cooking loss of cattle slaughtered for traditional ceremonies. Non-descript and Angus cattle slaughtered at low throughput abattoir had an effect on the colour of the beef in Table 4.2. The lightness (27.49 ± 2.53) and yellowness (21.16 ± 0.79) of the beef shows that there was a significant breed effect on cattle that are slaughtered at Low throughput abattoir, however redness of the meat was not affected by breed after slaughter. Cattle that are slaughtered at high throughput abattoir between ages of 6 to 8 years and also cattle that are more than 8 years in Table 4.4 had an effect on cooking loss (p < 0.05). Age between 6 to 8 years had an effect on the beef tenderness from cattle that are slaughtered at medium throughput abattoir. It was concluded that the meat tenderness of the young cattle with milk teeth slaughtered for traditional ceremonies was affected. On the other hand the meat tenderness and cooking loss of beef from the cattle slaughtered from low, medium and high throughput abattoir were affected by sex and breed mostly.
- Full Text:
- Date Issued: 2014
- Authors: Soga, Sizwe William
- Date: 2014
- Subjects: Meat -- Quality , Consumers' preferences , Consumers -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc Agric (Animal Science)
- Identifier: vital:11833 , http://hdl.handle.net/10353/d1019812 , Meat -- Quality , Consumers' preferences , Consumers -- South Africa -- Eastern Cape
- Description: The objective of the study was to determine perceptions of the consumers on how they judged behaviour and beef quality of cattle slaughtered for traditional ceremonies in the Eastern Cape Province of South Africa. A survey was conducted where a sample of 200 consumers were randomly selected and interviewed. Fifty non-descript cattle were slaughtered during traditional ceremonies and two hundred and twenty four (224) cattle were slaughtered at 3 through-put abattoirs (high, medium and low). Meat quality attributes were determined from the cattle slaughtered for traditional ceremonies and from cattle slaughtered at the abattoirs. Data for questionnaires was summarized as frequencies for each ceremony and statistical differences were analysed using chi square test (X2). The effect of Age, breed and sex on beef pH, colour, meat tenderness, temperature and cooking loss of cattle slaughtered during traditional ceremonies, at low, medium and high throughput abattoirs were analyzed using General Linear Model (PROG GLM) of SAS (2003). There was a significant age effect (P < 0.05) on beef tenderness and cooking loss of cattle slaughtered for traditional ceremonies. Age of cattle that have milk teeth to 1 year and age of 6 years to 8 years had an effect on the beef tenderness (25.55 ± 8.05) and cooking loss (48.00 ± 4.26) of cattle slaughtered for traditional ceremonies in Table 4.1. There was no significant sex effect on beef tenderness and cooking loss of cattle slaughtered for traditional ceremonies. Non-descript and Angus cattle slaughtered at low throughput abattoir had an effect on the colour of the beef in Table 4.2. The lightness (27.49 ± 2.53) and yellowness (21.16 ± 0.79) of the beef shows that there was a significant breed effect on cattle that are slaughtered at Low throughput abattoir, however redness of the meat was not affected by breed after slaughter. Cattle that are slaughtered at high throughput abattoir between ages of 6 to 8 years and also cattle that are more than 8 years in Table 4.4 had an effect on cooking loss (p < 0.05). Age between 6 to 8 years had an effect on the beef tenderness from cattle that are slaughtered at medium throughput abattoir. It was concluded that the meat tenderness of the young cattle with milk teeth slaughtered for traditional ceremonies was affected. On the other hand the meat tenderness and cooking loss of beef from the cattle slaughtered from low, medium and high throughput abattoir were affected by sex and breed mostly.
- Full Text:
- Date Issued: 2014
Socio-economic importance, health and welfare aspects of donkeys (Equus asinus) used for carting in a peri-urban area of South Africa
- Authors: Marufu, Madure
- Date: 2014
- Subjects: Donkeys -- South Africa , Animal welfare , Donkeys -- Handling , Donkeys -- Health
- Language: English
- Type: Thesis , Masters , MSc Agric (Animal Science)
- Identifier: vital:11832 , http://hdl.handle.net/10353/d1019781 , Donkeys -- South Africa , Animal welfare , Donkeys -- Handling , Donkeys -- Health
- Description: The broad objective of the study was to determine the socio-economic importance, health and welfare aspects of donkeys used for carting in a peri-urban area. A survey was conducted among 71 donkey owners in Joza, a peri-urban area near Grahamstown in the Eastern Cape Province (ECP) of South Africa to investigate their perceptions on socio-economic importance, health and welfare aspects of donkeys used for transport. Two hundred and seventy-one donkeys used for carting were assessed through direct observation of health and behaviour parameters to determine their welfare status. Results revealed that donkeys are indeed of immense value to people, being used daily (54.93%) for income generation activities and at least once per month (7.08%) for domestic chores. Most of the respondents (32%) earned R 600.00 per week while 11% earned at least R 200.00 per week from carting using donkeys. Donkeys were used for carting of goods (90.14%) more than for manure (16.9%). Gender and age of owners significantly (P < 0.05) influence perceptions on source of income while education level influenced their perceptions on carting firewood, goods and water. Results on management practices and health problems encountered by working donkeys showed that most of the owners managed their animals poorly. Most donkeys were being beaten (74.65%) during work, made to travel long distances (43.66%), worked long hours (52.11%) without adequate water (59.10%) and feed supplements (83.10%). Wounds (95.97%), coughing (75.65%) and lameness (64.79%) were some of the health problems encountered by donkeys. Significant relationships (P ˂ 0.05) were observed between owners’ perceptions on management practices and health problems encountered by donkeys. Among the observed animals, 61% were thin and mostly apathetic (26.2%) than medium and fat animals. Responses to observer approaches were significantly associated with sex and body condition score (BCS), with stallions in good body condition being more aggressive. Donkeys having a BCS of 3-5 avoided chin contact either by kicking out or moving away. Results further showed that most of the donkeys were suffering from external injuries, with the hindquarters (39.85%), shoulder (32.10%) and spine (21.77%) being the mostly affected areas. The prevalence of wounds on tail-base, belly, flank and neck were seen in less than 13% of the animals. The prevalence of wounds was influenced by age and BCS of the animals. Young donkeys with good BCS were less affected than old donkeys. Lameness, poor coat condition, external parasites, abnormal mucous membrane and dental problems were observed in less than 30% of the animals. It was concluded that donkeys play a pivotal role economically and socially to the livelihoods of people in a peri-urban area (Joza) in terms of income generation and transportation. However, these animals are experiencing multiple health and welfare problems. Therefore, more access to veterinary services, training on donkey use and management, health and welfare promotion programs are of paramount importance in solving the problem of poor health and welfare in donkeys.
- Full Text:
- Date Issued: 2014
- Authors: Marufu, Madure
- Date: 2014
- Subjects: Donkeys -- South Africa , Animal welfare , Donkeys -- Handling , Donkeys -- Health
- Language: English
- Type: Thesis , Masters , MSc Agric (Animal Science)
- Identifier: vital:11832 , http://hdl.handle.net/10353/d1019781 , Donkeys -- South Africa , Animal welfare , Donkeys -- Handling , Donkeys -- Health
- Description: The broad objective of the study was to determine the socio-economic importance, health and welfare aspects of donkeys used for carting in a peri-urban area. A survey was conducted among 71 donkey owners in Joza, a peri-urban area near Grahamstown in the Eastern Cape Province (ECP) of South Africa to investigate their perceptions on socio-economic importance, health and welfare aspects of donkeys used for transport. Two hundred and seventy-one donkeys used for carting were assessed through direct observation of health and behaviour parameters to determine their welfare status. Results revealed that donkeys are indeed of immense value to people, being used daily (54.93%) for income generation activities and at least once per month (7.08%) for domestic chores. Most of the respondents (32%) earned R 600.00 per week while 11% earned at least R 200.00 per week from carting using donkeys. Donkeys were used for carting of goods (90.14%) more than for manure (16.9%). Gender and age of owners significantly (P < 0.05) influence perceptions on source of income while education level influenced their perceptions on carting firewood, goods and water. Results on management practices and health problems encountered by working donkeys showed that most of the owners managed their animals poorly. Most donkeys were being beaten (74.65%) during work, made to travel long distances (43.66%), worked long hours (52.11%) without adequate water (59.10%) and feed supplements (83.10%). Wounds (95.97%), coughing (75.65%) and lameness (64.79%) were some of the health problems encountered by donkeys. Significant relationships (P ˂ 0.05) were observed between owners’ perceptions on management practices and health problems encountered by donkeys. Among the observed animals, 61% were thin and mostly apathetic (26.2%) than medium and fat animals. Responses to observer approaches were significantly associated with sex and body condition score (BCS), with stallions in good body condition being more aggressive. Donkeys having a BCS of 3-5 avoided chin contact either by kicking out or moving away. Results further showed that most of the donkeys were suffering from external injuries, with the hindquarters (39.85%), shoulder (32.10%) and spine (21.77%) being the mostly affected areas. The prevalence of wounds on tail-base, belly, flank and neck were seen in less than 13% of the animals. The prevalence of wounds was influenced by age and BCS of the animals. Young donkeys with good BCS were less affected than old donkeys. Lameness, poor coat condition, external parasites, abnormal mucous membrane and dental problems were observed in less than 30% of the animals. It was concluded that donkeys play a pivotal role economically and socially to the livelihoods of people in a peri-urban area (Joza) in terms of income generation and transportation. However, these animals are experiencing multiple health and welfare problems. Therefore, more access to veterinary services, training on donkey use and management, health and welfare promotion programs are of paramount importance in solving the problem of poor health and welfare in donkeys.
- Full Text:
- Date Issued: 2014
- «
- ‹
- 1
- ›
- »