Incidence of porcine circovirus type 2 and porcine parvoviruses in swine herds of some communities in Eastern Cape, South Africa
- Authors: Afolabi, Kayode Olayinka
- Date: 2018
- Subjects: Swine -- Diseases Swine -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10353/9691 , vital:34820
- Description: Porcine circovirus type 2 (PCV2) is one of the swine pathogens of global economic importance. Since its first detection in early 1990s as the main etiologic agent of porcine multisystemic wasting syndrome (PMWS) and many other porcine circovirus-associated diseases (PCVAD), the virus has been extensively studied and has been found to be present in virtually all the pig producing countries of the world. As a viral pathogen that brings about clinical diseases aided by co-infecting pathogens, the involvement of many other viral agents including porcine parvoviruses (PPVs) have caught the attention of stakeholders worldwide. However, no surveillance study of the viral pathogens has been carried out in South Africa as there are little or no information on their prevalence in the swine herds of the country. This present study therefore aimed at detection and molecular characterization of PCV2 and PPVs in swine herds of some selected communities in Eastern Cape Province, South Africa. A total of 375 field samples were collected from seven commercial and communal farms from three District Municipalities of Eastern Cape, South Africa between 2015 and 2016. Structured questionnaires were also administered to each farm at the time of sample collection to obtain some important information relating to health status and farm management practices in the sampled farms. With the aid of conventional PCR method, 339 samples were initially screened for the presence of PCV2; positive amplicons were sequenced and obtained partial genomes of the virus were preliminarily analyzed. In order to obtain the complete genomes of the virus, four overlapping primer pairs were used to amplify the full-genome of PCV2 from the initial positive samples; amplified genomes were sequenced using the Sanger methods, sequenced PCV2 genomes were assembled and characterized. Furthermore, the prevalences of some designated PPVs in the sampled farms were obtained using 110 samples randomly selected from the previously archived samples and screened with 6 different primer pairs specific for the detection of 7 PPVs. All the amplified parvoviruses’ genomes were sequenced; their sequenced partial genomes were subsequently base-culled and analysed. The data obtained revealed that 54/339 (15.93 percent) samples from the swine herds were positive for PCV2; whereas the degree of occurrence of the viral pathogen as observed at farm level ranges from approximately 5.6 to 60 percent. The majority 15/17 (88 percent) of the analyzed partial sequences were found clustering with other PCV2b strains in the phylogenetic analysis. More interestingly, two other sequences obtained were also found clustering within PCV2d genotype in the initial screening and analysis. Furthermore, 15 complete PCV2 genomes were successfully amplified, sequenced and assembled. NJ and ML phylogenetic analysis of the complete ORF2 gene and full genomes unanimously showed 11 of the assembled genomes belonging to genotype PCV2b. Another 3 of the characterized sequences formed clade with other reference mutant PCV2b and PCV2b subtype 1C (PCV2d) strains from different parts of the world. The last sequence however, clustered with other reference strains belonging to PCV2 intermediate clade 2 (PCV2-IM2) recently identified in a global PCV2 strains phylogenetic analysis. Other genetic analyses including multiple sequence alignment and p-distance analysis also confirmed the outcomes of the phylogenetic analyses of the complete capsid gene and fullgenomes of the virus. On the other hand, the findings of the molecular profiling for PPVs showed that all the screened parvoviruses were present in the study area, having prevalence of 29.1 percent (PPV1), 21.8 percent (PPV2), 5.5 percent (PPV3), 43.6 percent (PPV4), 21.8 percent (PBo-likeV) and 44.6 percent for PBoV1 and PBoV2. Double infection of the screened PPVs was observed to be very rampant among the pigs as high as 20/110 (18.2 percent) for PPV2/PPV4 and PPV4/PBoV; followed by 19/110 (17.3 percent) of the samples for PPV1/PPV4 and PPV1/PBoV. Three of the viruses were found simultaneously in 19 of the screened samples representing 17.3 percent, whereas 8 (7.3 percent) samples were positive for four of the viruses. Phylogenetic analyses of PPV1, PPV2 and PBoVs 1 and 2 were conducted with two major clades homologous for each of them. This is the first report of PCV2 in swine herds of the Province and the first detection of PCV2b, PCV2d and PCV2-IM2 strains in South African swine herds. It follows the first reported case of PCV2a in an outbreak of porcine multisystemic wasting syndrome (PMWS) in Gauteng Province, South Africa over two decades ago. Also, this is the first major epidemiologic study on PPVs in the country following the initial case study of 1975. These findings confirmed the presence of the allimportant viral pathogens among pigs and also give preliminary insights into the possibility of co-infections of the pathogens in the studied area. This could however result in a serious large scale outbreak of devastating disease(s) associated with the viral pathogens, thereby ultimately resulting in huge economic losses if no appropriate measures are taken to effectively curb their spread across the country.
- Full Text:
- Authors: Afolabi, Kayode Olayinka
- Date: 2018
- Subjects: Swine -- Diseases Swine -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10353/9691 , vital:34820
- Description: Porcine circovirus type 2 (PCV2) is one of the swine pathogens of global economic importance. Since its first detection in early 1990s as the main etiologic agent of porcine multisystemic wasting syndrome (PMWS) and many other porcine circovirus-associated diseases (PCVAD), the virus has been extensively studied and has been found to be present in virtually all the pig producing countries of the world. As a viral pathogen that brings about clinical diseases aided by co-infecting pathogens, the involvement of many other viral agents including porcine parvoviruses (PPVs) have caught the attention of stakeholders worldwide. However, no surveillance study of the viral pathogens has been carried out in South Africa as there are little or no information on their prevalence in the swine herds of the country. This present study therefore aimed at detection and molecular characterization of PCV2 and PPVs in swine herds of some selected communities in Eastern Cape Province, South Africa. A total of 375 field samples were collected from seven commercial and communal farms from three District Municipalities of Eastern Cape, South Africa between 2015 and 2016. Structured questionnaires were also administered to each farm at the time of sample collection to obtain some important information relating to health status and farm management practices in the sampled farms. With the aid of conventional PCR method, 339 samples were initially screened for the presence of PCV2; positive amplicons were sequenced and obtained partial genomes of the virus were preliminarily analyzed. In order to obtain the complete genomes of the virus, four overlapping primer pairs were used to amplify the full-genome of PCV2 from the initial positive samples; amplified genomes were sequenced using the Sanger methods, sequenced PCV2 genomes were assembled and characterized. Furthermore, the prevalences of some designated PPVs in the sampled farms were obtained using 110 samples randomly selected from the previously archived samples and screened with 6 different primer pairs specific for the detection of 7 PPVs. All the amplified parvoviruses’ genomes were sequenced; their sequenced partial genomes were subsequently base-culled and analysed. The data obtained revealed that 54/339 (15.93 percent) samples from the swine herds were positive for PCV2; whereas the degree of occurrence of the viral pathogen as observed at farm level ranges from approximately 5.6 to 60 percent. The majority 15/17 (88 percent) of the analyzed partial sequences were found clustering with other PCV2b strains in the phylogenetic analysis. More interestingly, two other sequences obtained were also found clustering within PCV2d genotype in the initial screening and analysis. Furthermore, 15 complete PCV2 genomes were successfully amplified, sequenced and assembled. NJ and ML phylogenetic analysis of the complete ORF2 gene and full genomes unanimously showed 11 of the assembled genomes belonging to genotype PCV2b. Another 3 of the characterized sequences formed clade with other reference mutant PCV2b and PCV2b subtype 1C (PCV2d) strains from different parts of the world. The last sequence however, clustered with other reference strains belonging to PCV2 intermediate clade 2 (PCV2-IM2) recently identified in a global PCV2 strains phylogenetic analysis. Other genetic analyses including multiple sequence alignment and p-distance analysis also confirmed the outcomes of the phylogenetic analyses of the complete capsid gene and fullgenomes of the virus. On the other hand, the findings of the molecular profiling for PPVs showed that all the screened parvoviruses were present in the study area, having prevalence of 29.1 percent (PPV1), 21.8 percent (PPV2), 5.5 percent (PPV3), 43.6 percent (PPV4), 21.8 percent (PBo-likeV) and 44.6 percent for PBoV1 and PBoV2. Double infection of the screened PPVs was observed to be very rampant among the pigs as high as 20/110 (18.2 percent) for PPV2/PPV4 and PPV4/PBoV; followed by 19/110 (17.3 percent) of the samples for PPV1/PPV4 and PPV1/PBoV. Three of the viruses were found simultaneously in 19 of the screened samples representing 17.3 percent, whereas 8 (7.3 percent) samples were positive for four of the viruses. Phylogenetic analyses of PPV1, PPV2 and PBoVs 1 and 2 were conducted with two major clades homologous for each of them. This is the first report of PCV2 in swine herds of the Province and the first detection of PCV2b, PCV2d and PCV2-IM2 strains in South African swine herds. It follows the first reported case of PCV2a in an outbreak of porcine multisystemic wasting syndrome (PMWS) in Gauteng Province, South Africa over two decades ago. Also, this is the first major epidemiologic study on PPVs in the country following the initial case study of 1975. These findings confirmed the presence of the allimportant viral pathogens among pigs and also give preliminary insights into the possibility of co-infections of the pathogens in the studied area. This could however result in a serious large scale outbreak of devastating disease(s) associated with the viral pathogens, thereby ultimately resulting in huge economic losses if no appropriate measures are taken to effectively curb their spread across the country.
- Full Text:
Influence of family structure on food security status of farming households in Alice, South Africa
- Authors: Ijila, Olusegun Jeremiah
- Date: 2018
- Subjects: Food security Families--South Africa--Eastern Cape Farms, Small
- Language: English
- Type: Thesis , Masters , Agricultural Economics
- Identifier: http://hdl.handle.net/10353/11537 , vital:39081
- Description: Achieving food security in sub-Saharan Africa remains a major challenge despite efforts made by a majority of the countries to reduce abject poverty and food insecurity. Just as these countries prioritized food security under the Millennium Development Goals (MDGs), they are equally putting it on top of the agenda under the Sustainable Development Goals (SDGs). In the face of the political and economic developments seen in the country since 1994, South Africa is overwhelmed by poverty and unemployment. But efforts to address these problems have not given due recognition to the question of family structure which is complex and varied, with immense potential implications for ownership and distribution of resources and the bargaining strength that are likely to determine the food security status of various farming households. Furthermore, the contribution of households towards agricultural production differs according to the way family organizes itself. This study was carried out to determine the influence of family structures on food security status of farming households in Alice, South Africa. Data were collected from 120 farming households’ selected using purposive sampling method. To collect data, a well-structured questionnaire was administered through face-to-face interviews. Consequent to that, the data were analyzed using descriptive statistics, and binary logistic regression model. The dominant family structures are nuclear, single-parent, working parent and cohabiting. The analysis revealed that nuclear family where father, mother and their children have access to labour and financial resources could be more comfortable than single parent family. Working parents would probably be more food secure because both parents are able to combine incomes while food insecurity might exist within cohabiting families since the union is less stable because of scarce resources occasioned by their inability to combine their resources to pursue a common goal. The results further revealed that single parent family structures and socio-economic characteristics like age, marital status and year of education of the household head were significant in terms of their influence on food security. The study therefore, recommends that old people should be encouraged to participate in agricultural production due to the wealth of experience gathered over the years. It is probably safe to conclude that encouraging marriage would provide better opportunities to raise the necessary capital to support positive productivity changes that would have implications for enhanced food availability and affordability.
- Full Text:
- Authors: Ijila, Olusegun Jeremiah
- Date: 2018
- Subjects: Food security Families--South Africa--Eastern Cape Farms, Small
- Language: English
- Type: Thesis , Masters , Agricultural Economics
- Identifier: http://hdl.handle.net/10353/11537 , vital:39081
- Description: Achieving food security in sub-Saharan Africa remains a major challenge despite efforts made by a majority of the countries to reduce abject poverty and food insecurity. Just as these countries prioritized food security under the Millennium Development Goals (MDGs), they are equally putting it on top of the agenda under the Sustainable Development Goals (SDGs). In the face of the political and economic developments seen in the country since 1994, South Africa is overwhelmed by poverty and unemployment. But efforts to address these problems have not given due recognition to the question of family structure which is complex and varied, with immense potential implications for ownership and distribution of resources and the bargaining strength that are likely to determine the food security status of various farming households. Furthermore, the contribution of households towards agricultural production differs according to the way family organizes itself. This study was carried out to determine the influence of family structures on food security status of farming households in Alice, South Africa. Data were collected from 120 farming households’ selected using purposive sampling method. To collect data, a well-structured questionnaire was administered through face-to-face interviews. Consequent to that, the data were analyzed using descriptive statistics, and binary logistic regression model. The dominant family structures are nuclear, single-parent, working parent and cohabiting. The analysis revealed that nuclear family where father, mother and their children have access to labour and financial resources could be more comfortable than single parent family. Working parents would probably be more food secure because both parents are able to combine incomes while food insecurity might exist within cohabiting families since the union is less stable because of scarce resources occasioned by their inability to combine their resources to pursue a common goal. The results further revealed that single parent family structures and socio-economic characteristics like age, marital status and year of education of the household head were significant in terms of their influence on food security. The study therefore, recommends that old people should be encouraged to participate in agricultural production due to the wealth of experience gathered over the years. It is probably safe to conclude that encouraging marriage would provide better opportunities to raise the necessary capital to support positive productivity changes that would have implications for enhanced food availability and affordability.
- Full Text:
Influence of trans-border trading activities on senior secondary school students' academic performance in economics in Ogun State, Nigeria
- Authors: Titus, Adesegun Benedict
- Date: 2018
- Subjects: Smuggling -- Education (Secondary) Borderlands -- Nigeria
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10353/9659 , vital:34814
- Description: The study investigated the influence of transborder trading activities on senior secondary school students’ academic performance in economics in Ogun state, Nigeria. A descriptive survey research design involving 320 students that comprised males and females were selected through stratified random sampling technique. Two self-developed instruments were used: student questionnaire on transborder trading activities (SQTTA) with a Cronbach alpha index of 0.7845; and the economics achievement test (EAT) with a co-efficient index of r= 0.814. The results of the data collected were collated, coded, analyzed and interpreted through the use of descriptive research statistics of frequency, mean, percentages, and standard deviation while Step-wise regression analysis was used to test the hypotheses raised in the study, and the inferential statistics t-test was used to test the significant differences between the independent and dependent variables. The results revealed that there is a positive significant influence of smuggling on students’ academic performance in economics concepts (t=4.123; df =307, p<0.05) and a negative significant influence of school location on students’ academic performance in economics concepts (t=0.952; df=307, p>0.05). The study revealed that the most prevalent transborder trading activities like smuggling of used tyres, small firearms and fairly used clothes had negative influence on students’ academic performance. Based on these findings, discussions were focused on the involvement of students in trans-border trading activities and the location of schools, amongst others, which has no significant influence on students’ academic performance. However, it was recommended that schools should not be located near border areas in future.
- Full Text:
- Authors: Titus, Adesegun Benedict
- Date: 2018
- Subjects: Smuggling -- Education (Secondary) Borderlands -- Nigeria
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10353/9659 , vital:34814
- Description: The study investigated the influence of transborder trading activities on senior secondary school students’ academic performance in economics in Ogun state, Nigeria. A descriptive survey research design involving 320 students that comprised males and females were selected through stratified random sampling technique. Two self-developed instruments were used: student questionnaire on transborder trading activities (SQTTA) with a Cronbach alpha index of 0.7845; and the economics achievement test (EAT) with a co-efficient index of r= 0.814. The results of the data collected were collated, coded, analyzed and interpreted through the use of descriptive research statistics of frequency, mean, percentages, and standard deviation while Step-wise regression analysis was used to test the hypotheses raised in the study, and the inferential statistics t-test was used to test the significant differences between the independent and dependent variables. The results revealed that there is a positive significant influence of smuggling on students’ academic performance in economics concepts (t=4.123; df =307, p<0.05) and a negative significant influence of school location on students’ academic performance in economics concepts (t=0.952; df=307, p>0.05). The study revealed that the most prevalent transborder trading activities like smuggling of used tyres, small firearms and fairly used clothes had negative influence on students’ academic performance. Based on these findings, discussions were focused on the involvement of students in trans-border trading activities and the location of schools, amongst others, which has no significant influence on students’ academic performance. However, it was recommended that schools should not be located near border areas in future.
- Full Text:
Inmate incarceration and rehabilitation : a cross sectional study of rehabilitation programmes and facilities in correctional centres in North Western Nigeria
- Authors: Amali, Sadiq Ewaoda
- Date: 2018
- Subjects: Criminal -- Rehabilitation Prisoners -- Counseling of Prisoners -- Mental health services
- Language: English
- Type: Thesis , Doctoral , Social Sciences
- Identifier: http://hdl.handle.net/10353/9984 , vital:35215
- Description: The study was conducted in Kano Central and Goron-Dutse prisons, North Western, Nigeria to determine the effectiveness of these correctional centres with regards to inmate rehabilitation. Both quantitative and qualitative collection was conducted concurrently and incorporated in the explanation of the results. This was achieved as the study empirically examined the experiences of convicted inmates and correctional officials in both correctional centres with a sample that compromised of both convicted male and female inmates as well as male and female correctional officials. A total of 250 questionnaires were administered to convicted inmates in Kano central and Goron-Dutse prisons. A sample size of 250 was determined using the sample size calculator at a confidence level of 95 percent, confidence interval of +/-5.97 percent, and with a population of 3488. Data for this study was generated from 250 respondents. Out of 250 respondents, questionnaires were administered to 140 inmates in Kano Central Prison and 110 questionnaires were administered to inmates in Goron Dutse Prison. The number of respondents in Kano Central Prisons is higher than that of Goron-Dutse Prison due to disparity in population of inmates. Also, 207 questionnaires were administered to correctional officials in Kano Central and Goron-Dutse prisons. A sample size of 207 was determined using the sample size calculator at a confidence interval of +/-5, confidence level of 95 percent and population of 449 correctional officials. Out of the 207 respondents, 107 questionnaires were administered to correctional officials in Kano Central Prison and 100 to correctional officials in Goron-Dutse Prison. The number of respondents in Kano Central Prison is higher than that of Goron-Dutse prison due to disparity in population of correctional officials. The selections were done using purposive sampling procedures. Rank, involvement in rehabilitation programmes were also used as criteria for selection of correctional officials as respondents. Furthermore, 28 correctional officials were selected purposively across the two correctional centres were interviewed. Thus, 14 correctional officials each were selected in Kano Central and Goron Dutse prisons using purposive sampling method. Thus, a total of 28 in-depth interviews were conducted in the study area. This study empirically probed into the factors mitigating against the effective rehabilitation of inmates. Therefore, against this background, this study involved an appraisal of rehabilitation programmes and facilities which was followed by an evaluation of inmates’ opinion of the correctional centre social setting and overall practices. Thus, inmate’s perceptions of correctional officials’ attitudes towards them and also its implication for re-integration principle was thoroughly appraised. The findings of this study were analyzed with the assistance of computer applications such as SPSS and atlas.ti.6. Some findings from this study revealed that only 15.7 percent of inmates in Kano Central prisons were satisfied with rehabilitation programmes, 37.3 percent were satisfied in Goron-Dutse prison while the majority of the inmates (88 percent) in Goron-Dutse prison stated that the relationship with correctional officials was cordial compared to 53.5 percent of the inmates in Kano Central prison. Also, data revealed that 64.2 percent of the inmates in Goron-Dutse Prison stated that medical attention was good and very good. The results also reveals that (58.9 percent) correctional officials stated that their relationship with inmates was very cordial compared to those of Goron-Dutse Prison (41.1 percent).Pearson Chi-square statistics showed that there was no statistical difference in correctional officials’ perceptions of the relationship with inmates between the two correctional centres (p.value>0.05). This study recommends that it is imperative for the Nigerian government to make more efforts with the provision of adequate rehabilitation programmes and facilities in the two correctional centres and correctional centres across the country as an exploration of existing literature reveals that most correctional centres in Nigeria lacked basic facilities for inmate rehabilitation as inmates were idle during incarceration.
- Full Text:
- Authors: Amali, Sadiq Ewaoda
- Date: 2018
- Subjects: Criminal -- Rehabilitation Prisoners -- Counseling of Prisoners -- Mental health services
- Language: English
- Type: Thesis , Doctoral , Social Sciences
- Identifier: http://hdl.handle.net/10353/9984 , vital:35215
- Description: The study was conducted in Kano Central and Goron-Dutse prisons, North Western, Nigeria to determine the effectiveness of these correctional centres with regards to inmate rehabilitation. Both quantitative and qualitative collection was conducted concurrently and incorporated in the explanation of the results. This was achieved as the study empirically examined the experiences of convicted inmates and correctional officials in both correctional centres with a sample that compromised of both convicted male and female inmates as well as male and female correctional officials. A total of 250 questionnaires were administered to convicted inmates in Kano central and Goron-Dutse prisons. A sample size of 250 was determined using the sample size calculator at a confidence level of 95 percent, confidence interval of +/-5.97 percent, and with a population of 3488. Data for this study was generated from 250 respondents. Out of 250 respondents, questionnaires were administered to 140 inmates in Kano Central Prison and 110 questionnaires were administered to inmates in Goron Dutse Prison. The number of respondents in Kano Central Prisons is higher than that of Goron-Dutse Prison due to disparity in population of inmates. Also, 207 questionnaires were administered to correctional officials in Kano Central and Goron-Dutse prisons. A sample size of 207 was determined using the sample size calculator at a confidence interval of +/-5, confidence level of 95 percent and population of 449 correctional officials. Out of the 207 respondents, 107 questionnaires were administered to correctional officials in Kano Central Prison and 100 to correctional officials in Goron-Dutse Prison. The number of respondents in Kano Central Prison is higher than that of Goron-Dutse prison due to disparity in population of correctional officials. The selections were done using purposive sampling procedures. Rank, involvement in rehabilitation programmes were also used as criteria for selection of correctional officials as respondents. Furthermore, 28 correctional officials were selected purposively across the two correctional centres were interviewed. Thus, 14 correctional officials each were selected in Kano Central and Goron Dutse prisons using purposive sampling method. Thus, a total of 28 in-depth interviews were conducted in the study area. This study empirically probed into the factors mitigating against the effective rehabilitation of inmates. Therefore, against this background, this study involved an appraisal of rehabilitation programmes and facilities which was followed by an evaluation of inmates’ opinion of the correctional centre social setting and overall practices. Thus, inmate’s perceptions of correctional officials’ attitudes towards them and also its implication for re-integration principle was thoroughly appraised. The findings of this study were analyzed with the assistance of computer applications such as SPSS and atlas.ti.6. Some findings from this study revealed that only 15.7 percent of inmates in Kano Central prisons were satisfied with rehabilitation programmes, 37.3 percent were satisfied in Goron-Dutse prison while the majority of the inmates (88 percent) in Goron-Dutse prison stated that the relationship with correctional officials was cordial compared to 53.5 percent of the inmates in Kano Central prison. Also, data revealed that 64.2 percent of the inmates in Goron-Dutse Prison stated that medical attention was good and very good. The results also reveals that (58.9 percent) correctional officials stated that their relationship with inmates was very cordial compared to those of Goron-Dutse Prison (41.1 percent).Pearson Chi-square statistics showed that there was no statistical difference in correctional officials’ perceptions of the relationship with inmates between the two correctional centres (p.value>0.05). This study recommends that it is imperative for the Nigerian government to make more efforts with the provision of adequate rehabilitation programmes and facilities in the two correctional centres and correctional centres across the country as an exploration of existing literature reveals that most correctional centres in Nigeria lacked basic facilities for inmate rehabilitation as inmates were idle during incarceration.
- Full Text:
Institutions, Production and Marketing of maize in Zimbabwe. Case of Marondera District of Mashonaland East Province
- Authors: Mafuse, Never
- Date: 2018
- Subjects: Food security Food supply
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10353/15687 , vital:40506
- Description: Food security situation in Zimbabwe is deteriorating despite the efforts made by the government for embarking on agrarian reforms in terms of redressing colonial imbalances and taking on the major trading investment risks. The government assured the resettled farmers that if they invest in maize production, they would not be stranded by lack of marketing services as the markets are liberalized. However, this market liberalization policy occurring in maize sector has resulted in A1 resettled farmers becoming more vulnerable to traders preying on their weaknesses as the policy is not living up to expectations and is failing to bring dramatic increases in agricultural growth. This may mean that sending children to school is a nightmare for those who depend on farming for educating their children, inadequate food in the home, unpaid loans and subsequent impoverishment. The resettled A1farmers will remain trapped in the poverty cycle as long as institutions are not corrected. The general aim of the study was to evaluate institutional factors influencing maize production and marketing by A1 resettled farmers of Marondera district in Mashonaland East province of Zimbabwe. Using a sample of 343 farmers randomly selected in Marondera District, different econometric models like Data envelopment Analysis, Cob Douglas, Binary logistic, and Tobit as well as descriptive statistics were used to analyse the data. Institutional variables were captured in two ways: as a dummy showing whether farmers have access to institutional variables and as a continuous variable indicating importance of institutions on maize production and marketing. The study found out that, most of the A1 farmers are old, with a mean age of 47 years and a maximum of 55 years of farming experience. It is also dominated by male farmers who are literate. Results also showed that most of A1 farmers from the sample are married and very few separated. These farmers sell most their maize and keep little for consumption though they are still using convectional farming methods. Most of the farmers from the sample generate their incomes from farming activities only and they have good access to market and production information as well as extension xvi messages through training .They however have poor access to financial credit and weak social capital as well as low contract farming. Results also indicated that A1 farmers pursue the formal supply chains than informal supply chains. It is noted that, A1 farmers participate in a number of markets that include agricultural inputs markets seed, fertilizers, chemicals.; credit markets which includes banks as well as labour markets which can in the form of (maricho). In terms production and marketing efficiency, the study found out that A1 farmers are technically, allocatively and economically inefficient. The maximum efficient score is 1 while the minimum score is 0.01 for both production and marketing stage. The mean scores for production stage are 0.195, 0.504 and 0.072 for technical, allocative and economic efficient respectively. The mean scores for marketing are 0.103, 0.688, and 0.068 for technical, allocative and economic efficiency respectively. Farmers are performing better at production stage than at marketing technically while there is better allocative and economic efficiency at marketing stage. Results for determinants of technical, allocative and economic efficiency at production stage showed that, cattle ownership, farming experience, access to financial credit and (member of farmer organisation) which was used as a proxy indicator for social capital ,access to information, contract farming, distance to input market ,area planted to maize and quantity of seed used influences marketing efficiency of A1 maize farmers in Marondera District. Access to credit, Access to market, distance to market, road infrastructure, farming experience and access to information influences technical, allocative and economic efficiency at marketing stage. Cooperative membership, road infrastructure, contractual farming arrangements, and access to financial credit and transport cost to market influence choice of marketing channel respectively. Finally results from the non linear cob Douglas reveals that, maize production is significantly influenced by contract with value chain actors, access to market xvii information, access to financial credit, total topdressing used , total basal fertilizer used, total herbicides used, and total area put under maize production. The results showed that, future of A1 resettled farmers depends on correcting the institutions which are of paramount importance for maize production and marketing. The study therefore recommended the government to improve institutional environment that has direct link with institutional innovations necessary for sustainable maize production and marketing. This can be done through correcting inefficiencies and ineffective institutions by creating and maintaining both environment and arrangement that will transform maize production and marketing systems to operate efficiently. The study presents policy options to address both production and marketing of maize arguing that new direction of production and marketing of maize depends on correcting dysfunctional institutions. Policy options include increasing A1 farmers access to financial credit by removing the stringent collateral requirements which are not attainable by most A1 farmers as their asset endowment is limited to non capital. Addressing imperfections in the input markets by improving the supply of chemical fertilisers and seed at affordable prices as this influences total area put under maize production. The other policy is for private companies and NGOs to offer contract farming to A1 maize farmers as this will help them to increase total area put under maize production, access to market, access to credit, and access to information and reduces long distances to purchase inputs. Introducing focused training to motivate resettled A1 farmers on formation farmer organisations as this assists in social capital which is an important asset in maize production and marketing.Improving road infrastructure by creating a fund that would be used to maintain the road rather waiting for DDF only.
- Full Text:
- Authors: Mafuse, Never
- Date: 2018
- Subjects: Food security Food supply
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10353/15687 , vital:40506
- Description: Food security situation in Zimbabwe is deteriorating despite the efforts made by the government for embarking on agrarian reforms in terms of redressing colonial imbalances and taking on the major trading investment risks. The government assured the resettled farmers that if they invest in maize production, they would not be stranded by lack of marketing services as the markets are liberalized. However, this market liberalization policy occurring in maize sector has resulted in A1 resettled farmers becoming more vulnerable to traders preying on their weaknesses as the policy is not living up to expectations and is failing to bring dramatic increases in agricultural growth. This may mean that sending children to school is a nightmare for those who depend on farming for educating their children, inadequate food in the home, unpaid loans and subsequent impoverishment. The resettled A1farmers will remain trapped in the poverty cycle as long as institutions are not corrected. The general aim of the study was to evaluate institutional factors influencing maize production and marketing by A1 resettled farmers of Marondera district in Mashonaland East province of Zimbabwe. Using a sample of 343 farmers randomly selected in Marondera District, different econometric models like Data envelopment Analysis, Cob Douglas, Binary logistic, and Tobit as well as descriptive statistics were used to analyse the data. Institutional variables were captured in two ways: as a dummy showing whether farmers have access to institutional variables and as a continuous variable indicating importance of institutions on maize production and marketing. The study found out that, most of the A1 farmers are old, with a mean age of 47 years and a maximum of 55 years of farming experience. It is also dominated by male farmers who are literate. Results also showed that most of A1 farmers from the sample are married and very few separated. These farmers sell most their maize and keep little for consumption though they are still using convectional farming methods. Most of the farmers from the sample generate their incomes from farming activities only and they have good access to market and production information as well as extension xvi messages through training .They however have poor access to financial credit and weak social capital as well as low contract farming. Results also indicated that A1 farmers pursue the formal supply chains than informal supply chains. It is noted that, A1 farmers participate in a number of markets that include agricultural inputs markets seed, fertilizers, chemicals.; credit markets which includes banks as well as labour markets which can in the form of (maricho). In terms production and marketing efficiency, the study found out that A1 farmers are technically, allocatively and economically inefficient. The maximum efficient score is 1 while the minimum score is 0.01 for both production and marketing stage. The mean scores for production stage are 0.195, 0.504 and 0.072 for technical, allocative and economic efficient respectively. The mean scores for marketing are 0.103, 0.688, and 0.068 for technical, allocative and economic efficiency respectively. Farmers are performing better at production stage than at marketing technically while there is better allocative and economic efficiency at marketing stage. Results for determinants of technical, allocative and economic efficiency at production stage showed that, cattle ownership, farming experience, access to financial credit and (member of farmer organisation) which was used as a proxy indicator for social capital ,access to information, contract farming, distance to input market ,area planted to maize and quantity of seed used influences marketing efficiency of A1 maize farmers in Marondera District. Access to credit, Access to market, distance to market, road infrastructure, farming experience and access to information influences technical, allocative and economic efficiency at marketing stage. Cooperative membership, road infrastructure, contractual farming arrangements, and access to financial credit and transport cost to market influence choice of marketing channel respectively. Finally results from the non linear cob Douglas reveals that, maize production is significantly influenced by contract with value chain actors, access to market xvii information, access to financial credit, total topdressing used , total basal fertilizer used, total herbicides used, and total area put under maize production. The results showed that, future of A1 resettled farmers depends on correcting the institutions which are of paramount importance for maize production and marketing. The study therefore recommended the government to improve institutional environment that has direct link with institutional innovations necessary for sustainable maize production and marketing. This can be done through correcting inefficiencies and ineffective institutions by creating and maintaining both environment and arrangement that will transform maize production and marketing systems to operate efficiently. The study presents policy options to address both production and marketing of maize arguing that new direction of production and marketing of maize depends on correcting dysfunctional institutions. Policy options include increasing A1 farmers access to financial credit by removing the stringent collateral requirements which are not attainable by most A1 farmers as their asset endowment is limited to non capital. Addressing imperfections in the input markets by improving the supply of chemical fertilisers and seed at affordable prices as this influences total area put under maize production. The other policy is for private companies and NGOs to offer contract farming to A1 maize farmers as this will help them to increase total area put under maize production, access to market, access to credit, and access to information and reduces long distances to purchase inputs. Introducing focused training to motivate resettled A1 farmers on formation farmer organisations as this assists in social capital which is an important asset in maize production and marketing.Improving road infrastructure by creating a fund that would be used to maintain the road rather waiting for DDF only.
- Full Text:
Investigation of strategies employed in rural households to improve food security in Raymond Mhlaba Local Municipality : a case of Zihlahleni Location in Middledrift, Eastern Cape Province
- Authors: Gqozo, Odwa
- Date: 2018
- Subjects: Food security -- South Africa -- Eastern Cape Rural development -- South Africa -- Eastern Cape Households -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/10758 , vital:35752
- Description: Eastern Cape is one of the provinces experiencing a rapid increase in food prices while household income is increasing at a slower rate. Against this background, the main objective of this study was to investigate strategies used by rural households to improve food security in Raymond Mhlaba Local Municipality formally known as Nkonkobe in the Eastern Cape Province, South Africa. Data were collected from 100 households using a questionnaire as the main instrument by using simple random sampling. Data were analyzed by employing simple descriptive statistics to describe household food security. Results revealed that most (50.3percent) households that were interviewed were male headed and unemployment was high (73.6percent). Moreover, the average age of the interviewed household heads was 59 years. Most (68percent) of the households owned home gardens and had access to arable land which was not being cultivated. Most (87percent) households indicated that lack of capital was the main cause of food security and depended on government grants. Most (100percent) of the interviewed household heads depend mostly on food purchases rather than their own production. The recommendation is that the government should continue fostering its agenda of improving agriculture as a strategy in rural households for curbing food insecurity.
- Full Text:
- Authors: Gqozo, Odwa
- Date: 2018
- Subjects: Food security -- South Africa -- Eastern Cape Rural development -- South Africa -- Eastern Cape Households -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/10758 , vital:35752
- Description: Eastern Cape is one of the provinces experiencing a rapid increase in food prices while household income is increasing at a slower rate. Against this background, the main objective of this study was to investigate strategies used by rural households to improve food security in Raymond Mhlaba Local Municipality formally known as Nkonkobe in the Eastern Cape Province, South Africa. Data were collected from 100 households using a questionnaire as the main instrument by using simple random sampling. Data were analyzed by employing simple descriptive statistics to describe household food security. Results revealed that most (50.3percent) households that were interviewed were male headed and unemployment was high (73.6percent). Moreover, the average age of the interviewed household heads was 59 years. Most (68percent) of the households owned home gardens and had access to arable land which was not being cultivated. Most (87percent) households indicated that lack of capital was the main cause of food security and depended on government grants. Most (100percent) of the interviewed household heads depend mostly on food purchases rather than their own production. The recommendation is that the government should continue fostering its agenda of improving agriculture as a strategy in rural households for curbing food insecurity.
- Full Text:
Investigation of the subsurface geology using time domain, magnetic and electrical resistivity geophysical techniques in the Karoo basin at Beaufort west, Western Cape Province, South Africa
- Authors: Ntunja, Asanda
- Date: 2018
- Subjects: Geology Geophysics
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/14659 , vital:40034
- Description: Beaufort West lies in the Central Karoo Basin and is specifically situated in the Poortjie Member of the Teekloof Formation in the Beaufort Group. The Teekloof Formation was deposited in a floodplain environment and it consists of alternating bluish grey mudstone and very fine to medium grained sandstone. The sediments of the Karoo Basin were intruded by numerous dolerite sills and dykes during a period of extensive magmatic activity over the whole South African subcontinent when the break-up of Gondwanda occurred. This led to a continuous jointing of the sedimentary rocks and the jointing resulted in rocks that are prone to weathering. Weathering that occurred in Beaufort West resulted in the formation of alluvium, calcrete, scree and hard pan deposits which overlay most of the ground surface. The joints in rocks paved way for groundwater storage in the sedimentary beds as sediments of the Karoo Basin lack significant primary porosity. Geophysical surveys were done in order to determine a suitable site to locate and drill a deep borehole to the White Hill Formation in order to extract shale gas that is proposed to be present in the area, thereafter determine sites for monitoring boreholes for groundwater and also look for area where potential groundwater might be present. This then requires geophysical investigations of the surface and subsurface geology of the area prior to drilling so as to locate any features such as dolerite intrusions which may hinder the drilling process and locate shallow aquifers, which will tend to be monitored before and during fracking. Five lines of varying lengths from 2-6 km and different orientation were surveyed using geophysical techniques which included the time domain, electrical resistivity as well as the magnetic methods. The geophysical investigations were conducted in order to fulfil the main vii objectives and aims of the study. The techniques were then complemented by XRF, petrography and density analyses. The time domain method reveals that Lines 1 and Line 2 comprises of rocks with low resistivity values of less than 30 Ωm. These two lines were more conductive towards the east and northeast side of the study site and the resistivity tends to increase towards the south and west. Line 4 appeared to be more conductive towards the south-east of the line. Lines 3 and 5 showed high resistivity values with Line 5 being the most resistive suggesting that the underlying strata is consolidated, dry and hard. The resistivity in all the lines surveyed showed an increase with increasing depth. The depth of investigation was approximately up to 150 m. The electrical resistivity results were only obtained from Line 1 and Line 2. The results, which complement the time domain results, suggest that the two lines indeed comprise of rocks which exhibit low resistivity values. This then implies that the rocks in the vicinity are fractured and the fractures might be field with groundwater and possibly contain some conductive minerals such as sodium oxide (Na2O) as supported by XRF results. This method also reveals that along Line 1, there are individual bodies of high resistivity values. The probing depth of about 78 m was achieved for the electrical resistivity survey. The magnetic data indicate that the magnetic intensity does not vary much across lines 3, 4 and 5 and there is not much change in lithology with depth and lateral extent. The area covered by Lines 1 and 2, and the additional eleven fill in lines has several prominent magnetic highs that are inferred to be due to dolerite intrusions in the sandstones and these bodies may be connected at depth. One of these anomalies which trends east-west direction partially coincides with a mapped dolerite intrusion. Some areas with sandstones also exhibit high viii magnetism which is due to about 3.3% content of Fe2O3 present in the sandstones as seen from XRF. This is probably resulted from weathering of magnetite in dolerite intrusions. Petrography results obtained from samples collected in the field reveal that there is an abundance of quartz in sandstones and siltstones. Quartz arenite also contained about 50% of feldspar, containing both plagioclase and K-feldspar. The sandstones of the Teekloof Formation (Beaufort West) have undergone calcite replacement where the calcite matrix replaced most or all of the clay matrix between the mineral grains. The fractures in rocks were seen and it was inferred that they are due to the present cleavages in feldspar and weathering of feldspar in sandstones. Through X-ray Fluorescence (XRF) analysis, it was revealed that the rocks contained a high percentage of Na2O. XRF also showed that these rocks were under low chemical weathering which led to the formation of new minerals in rock as other minerals such as feldspar weather away. This resulted in quartz being the most dominant mineral present as it is not prone to chemical weathering and thus the rocks were proven to be chemically matured. Thirty one rock samples were collected from the field in order to determine the density and porosity measurements. The fine to medium grained grey sandstones show an average density of 2.597 g/cm3 and a porosity of 1.4 %. The brownish grey sandstone have an average density of 2.546 g/cm3 and porosity of 2.9 % and the brown sandstones exhibit an average of 2.584 g/cm3 and a porosity 1.46 %. Dolerite has an average density of 2.970 g/cm3 and porosity of 0.5 % and siltstones have a density of 2.595 g/cm3 and porosity of 1.38 %. The brownish grey sandstones have the highest porosity and the grey sandstone and siltstone have the least porosity after the dolerite.
- Full Text:
- Authors: Ntunja, Asanda
- Date: 2018
- Subjects: Geology Geophysics
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/14659 , vital:40034
- Description: Beaufort West lies in the Central Karoo Basin and is specifically situated in the Poortjie Member of the Teekloof Formation in the Beaufort Group. The Teekloof Formation was deposited in a floodplain environment and it consists of alternating bluish grey mudstone and very fine to medium grained sandstone. The sediments of the Karoo Basin were intruded by numerous dolerite sills and dykes during a period of extensive magmatic activity over the whole South African subcontinent when the break-up of Gondwanda occurred. This led to a continuous jointing of the sedimentary rocks and the jointing resulted in rocks that are prone to weathering. Weathering that occurred in Beaufort West resulted in the formation of alluvium, calcrete, scree and hard pan deposits which overlay most of the ground surface. The joints in rocks paved way for groundwater storage in the sedimentary beds as sediments of the Karoo Basin lack significant primary porosity. Geophysical surveys were done in order to determine a suitable site to locate and drill a deep borehole to the White Hill Formation in order to extract shale gas that is proposed to be present in the area, thereafter determine sites for monitoring boreholes for groundwater and also look for area where potential groundwater might be present. This then requires geophysical investigations of the surface and subsurface geology of the area prior to drilling so as to locate any features such as dolerite intrusions which may hinder the drilling process and locate shallow aquifers, which will tend to be monitored before and during fracking. Five lines of varying lengths from 2-6 km and different orientation were surveyed using geophysical techniques which included the time domain, electrical resistivity as well as the magnetic methods. The geophysical investigations were conducted in order to fulfil the main vii objectives and aims of the study. The techniques were then complemented by XRF, petrography and density analyses. The time domain method reveals that Lines 1 and Line 2 comprises of rocks with low resistivity values of less than 30 Ωm. These two lines were more conductive towards the east and northeast side of the study site and the resistivity tends to increase towards the south and west. Line 4 appeared to be more conductive towards the south-east of the line. Lines 3 and 5 showed high resistivity values with Line 5 being the most resistive suggesting that the underlying strata is consolidated, dry and hard. The resistivity in all the lines surveyed showed an increase with increasing depth. The depth of investigation was approximately up to 150 m. The electrical resistivity results were only obtained from Line 1 and Line 2. The results, which complement the time domain results, suggest that the two lines indeed comprise of rocks which exhibit low resistivity values. This then implies that the rocks in the vicinity are fractured and the fractures might be field with groundwater and possibly contain some conductive minerals such as sodium oxide (Na2O) as supported by XRF results. This method also reveals that along Line 1, there are individual bodies of high resistivity values. The probing depth of about 78 m was achieved for the electrical resistivity survey. The magnetic data indicate that the magnetic intensity does not vary much across lines 3, 4 and 5 and there is not much change in lithology with depth and lateral extent. The area covered by Lines 1 and 2, and the additional eleven fill in lines has several prominent magnetic highs that are inferred to be due to dolerite intrusions in the sandstones and these bodies may be connected at depth. One of these anomalies which trends east-west direction partially coincides with a mapped dolerite intrusion. Some areas with sandstones also exhibit high viii magnetism which is due to about 3.3% content of Fe2O3 present in the sandstones as seen from XRF. This is probably resulted from weathering of magnetite in dolerite intrusions. Petrography results obtained from samples collected in the field reveal that there is an abundance of quartz in sandstones and siltstones. Quartz arenite also contained about 50% of feldspar, containing both plagioclase and K-feldspar. The sandstones of the Teekloof Formation (Beaufort West) have undergone calcite replacement where the calcite matrix replaced most or all of the clay matrix between the mineral grains. The fractures in rocks were seen and it was inferred that they are due to the present cleavages in feldspar and weathering of feldspar in sandstones. Through X-ray Fluorescence (XRF) analysis, it was revealed that the rocks contained a high percentage of Na2O. XRF also showed that these rocks were under low chemical weathering which led to the formation of new minerals in rock as other minerals such as feldspar weather away. This resulted in quartz being the most dominant mineral present as it is not prone to chemical weathering and thus the rocks were proven to be chemically matured. Thirty one rock samples were collected from the field in order to determine the density and porosity measurements. The fine to medium grained grey sandstones show an average density of 2.597 g/cm3 and a porosity of 1.4 %. The brownish grey sandstone have an average density of 2.546 g/cm3 and porosity of 2.9 % and the brown sandstones exhibit an average of 2.584 g/cm3 and a porosity 1.46 %. Dolerite has an average density of 2.970 g/cm3 and porosity of 0.5 % and siltstones have a density of 2.595 g/cm3 and porosity of 1.38 %. The brownish grey sandstones have the highest porosity and the grey sandstone and siltstone have the least porosity after the dolerite.
- Full Text:
Isolation and characterization of E. coli and Campylobacter spp. from diarrhoeal samples collected from selected hospitals in Amathole District Municipality, Eastern Cape, South Africa
- Authors: Omolajaiye, Sunday Abraham
- Date: 2018
- Subjects: Escherichia coli infections Campylobacter infections Diarrhea
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/6213 , vital:29515
- Description: Approximately 2-4 billion cases of infectious diarrhoea occur every year, with the highest numbers recorded in sub-Saharan Africa. It remains the most common public health issue among children in developing nations. The purpose of this research was to unfold the prevalence of diarrhoeagenic E. coli and Campylobacter pathotypes as well as elucidate their antibiogram characteristics in diarrhoeal stool samples collected in some medical facilities in Eastern Cape Province, South Africa. Two hundred stool samples were collected from both inpatients and outpatients from male and females of all age groups attending selected medical facilities in the study area. Isolation and characterization of both organisms were done using culture based and molecular methods. Antibiotic susceptibility patterns of identified isolates were determined against a panel of 12 antimicrobial agents. One hundred and twenty presumptive E. coli isolates and 42 presumptive isolates of Campylobacter spp. Were isolated. Eighty-two percent (82 percent) of the presumptive E. coli isolates were confirmed as E. coli while 46.3 percent belonged to Campylobacter spp. Pathotyping of the diarrhoeagenic E. coli isolates by Polymerase chain reaction (PCR) showed the following prevalences: DAEC 43 (32 percent), EHEC 18 (17 percent), EIEC 11 (10 percent) and EPEC 18 (17 percent). EAEC and ETEC were not detected, while for Campylobacter spp. 37 (88 percent) were C. jejuni, and C. coli was not detected. A total of 12 (32.4 percent) of the confirmed Campylobacter jejuni isolates were found to possess the fliM gene, 9 (24.3 percent) possessed the flhA gene and only 6 (16.2 percent) harboured the gene flgE2. None were positive for the flaA, flab and flhB genes.The antibiotic resistance patterns observed among the E. coli isolates were high against ampicillin (98.1 percent), chloramphenicol (94.3 percent) and tetracycline (90.6 percent). For Campylobacter spp., resistance observed were: chloramphenicol (91.6 percent), tetracycline (25.2 percent), erythromycin (49.6 percent) and gentamycin (56.4 percent). A lesser resistance against imipenem (35.9 percent) and quinolone (ciprofloxacin) (45.5 percent) were exhibited by the E.coli isolates. 10.8 percent and 20.3 percent of the Campylobacter isolates were resistant to imipenem and ciprofloxacin respectively. The presence of chloramphenicol (CatA1) and tetracycline (tetA) resistance genes were detected in 94 percent and 89 percent of E. coli isolates respectively while 98 percent of Campylobacter spp. Harboured the catA1 resistance gene. It could be deduced from this study that E. coli and Campylobacter spp. are predomiant enteric pathogens as the etiologic agents of diarrhoea in the study community, and that their antimicrobial resistance is high in the study location. The need to develop strategies to prevent infection and control resistant organisms is evident.
- Full Text:
- Authors: Omolajaiye, Sunday Abraham
- Date: 2018
- Subjects: Escherichia coli infections Campylobacter infections Diarrhea
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/6213 , vital:29515
- Description: Approximately 2-4 billion cases of infectious diarrhoea occur every year, with the highest numbers recorded in sub-Saharan Africa. It remains the most common public health issue among children in developing nations. The purpose of this research was to unfold the prevalence of diarrhoeagenic E. coli and Campylobacter pathotypes as well as elucidate their antibiogram characteristics in diarrhoeal stool samples collected in some medical facilities in Eastern Cape Province, South Africa. Two hundred stool samples were collected from both inpatients and outpatients from male and females of all age groups attending selected medical facilities in the study area. Isolation and characterization of both organisms were done using culture based and molecular methods. Antibiotic susceptibility patterns of identified isolates were determined against a panel of 12 antimicrobial agents. One hundred and twenty presumptive E. coli isolates and 42 presumptive isolates of Campylobacter spp. Were isolated. Eighty-two percent (82 percent) of the presumptive E. coli isolates were confirmed as E. coli while 46.3 percent belonged to Campylobacter spp. Pathotyping of the diarrhoeagenic E. coli isolates by Polymerase chain reaction (PCR) showed the following prevalences: DAEC 43 (32 percent), EHEC 18 (17 percent), EIEC 11 (10 percent) and EPEC 18 (17 percent). EAEC and ETEC were not detected, while for Campylobacter spp. 37 (88 percent) were C. jejuni, and C. coli was not detected. A total of 12 (32.4 percent) of the confirmed Campylobacter jejuni isolates were found to possess the fliM gene, 9 (24.3 percent) possessed the flhA gene and only 6 (16.2 percent) harboured the gene flgE2. None were positive for the flaA, flab and flhB genes.The antibiotic resistance patterns observed among the E. coli isolates were high against ampicillin (98.1 percent), chloramphenicol (94.3 percent) and tetracycline (90.6 percent). For Campylobacter spp., resistance observed were: chloramphenicol (91.6 percent), tetracycline (25.2 percent), erythromycin (49.6 percent) and gentamycin (56.4 percent). A lesser resistance against imipenem (35.9 percent) and quinolone (ciprofloxacin) (45.5 percent) were exhibited by the E.coli isolates. 10.8 percent and 20.3 percent of the Campylobacter isolates were resistant to imipenem and ciprofloxacin respectively. The presence of chloramphenicol (CatA1) and tetracycline (tetA) resistance genes were detected in 94 percent and 89 percent of E. coli isolates respectively while 98 percent of Campylobacter spp. Harboured the catA1 resistance gene. It could be deduced from this study that E. coli and Campylobacter spp. are predomiant enteric pathogens as the etiologic agents of diarrhoea in the study community, and that their antimicrobial resistance is high in the study location. The need to develop strategies to prevent infection and control resistant organisms is evident.
- Full Text:
Knowledge, attitudes and practice of professional nurses regarding organ donation in critical care units of public and private hospitals in Eastern Cape Province
- Authors: Green, Bukelwa
- Date: 2018
- Subjects: Organ donors--Supply and demand Donation of organs, tissues, etc Transplantation of organs, tissues
- Language: English
- Type: Thesis , Masters , Magister Curationis
- Identifier: http://hdl.handle.net/10353/11116 , vital:37138
- Description: Globally there is an overwhelming increase in the number of patients waiting for donated organs for transplantation, with a gross shortage of available organs. There are many contributing factors to this dearth of donated organs, including level of knowledge, cultural beliefs, lack of clear practice and the attitudes of health care providers, as the gatekeepers of the health system. This non-experimental research study assessed the attitudes, level of knowledge, and practices of professional nurses working in critical care units in public and private hospitals in Eastern Cape Province regarding organ donation. The study was conducted in both private and public hospital intensive care and renal care units. The targeted population in the study was professional nurses working in intensive and renal care units, trained in critical care or nephrology, as well as those who were not trained but working in these specialised areas. The non-probability, purposive sampling method was employed and total population was 187 professional nurses working in public and private critical care units but only 108 managed to yield data for this study. Data was collected with anonymous, self - developed questionnaires. The questionnaire was submitted to supervisors and statistician for validity. Reliability was established by conducting pretest of the instrument, which yielded positive results because all questionnaires were correctly completed.Data captured and analysed using the Statistical Package for Social Sciences (SPSS) software for Windows version 22 and is presented by means of percentages and tables. Results: The findings were that, there is insufficient knowledge on the topic of organ donation, with subsequent inadequate practice, which derives from lack of pre-registration training and ongoing in-service education regarding the topic. The unavailability of full organ transplantation services in the province is viewed as a hindrance in the practice of organ donation. There was generally a positive attitude towards organ donation, particularly among Christian nurses. The knowledge deficit and limited practice seem not to have affected the attitudes of the participants, who supported the idea of organ donation. However, despite the positive attitudes, only 13 participants (12.0 percent) agreed to be registered as organ donors. 50 percent of participants lacked knowledge about availability and contact details of the provincial organ donation foundation, which may indicate inadequate marketing on the part of the foundation. There was no significant difference in knowledge, attitudes and practice between nurses in the private and public sectors. Rather differences were noted between the different levels of health care services, i.e. between the secondary and tertiary level. The tertiary level outperformed the secondary level institutions. It is recommended that organ donation be incorporated into the syllabi of health sciences courses both in universities and colleges, and that awareness campaigns be strengthened.
- Full Text:
- Authors: Green, Bukelwa
- Date: 2018
- Subjects: Organ donors--Supply and demand Donation of organs, tissues, etc Transplantation of organs, tissues
- Language: English
- Type: Thesis , Masters , Magister Curationis
- Identifier: http://hdl.handle.net/10353/11116 , vital:37138
- Description: Globally there is an overwhelming increase in the number of patients waiting for donated organs for transplantation, with a gross shortage of available organs. There are many contributing factors to this dearth of donated organs, including level of knowledge, cultural beliefs, lack of clear practice and the attitudes of health care providers, as the gatekeepers of the health system. This non-experimental research study assessed the attitudes, level of knowledge, and practices of professional nurses working in critical care units in public and private hospitals in Eastern Cape Province regarding organ donation. The study was conducted in both private and public hospital intensive care and renal care units. The targeted population in the study was professional nurses working in intensive and renal care units, trained in critical care or nephrology, as well as those who were not trained but working in these specialised areas. The non-probability, purposive sampling method was employed and total population was 187 professional nurses working in public and private critical care units but only 108 managed to yield data for this study. Data was collected with anonymous, self - developed questionnaires. The questionnaire was submitted to supervisors and statistician for validity. Reliability was established by conducting pretest of the instrument, which yielded positive results because all questionnaires were correctly completed.Data captured and analysed using the Statistical Package for Social Sciences (SPSS) software for Windows version 22 and is presented by means of percentages and tables. Results: The findings were that, there is insufficient knowledge on the topic of organ donation, with subsequent inadequate practice, which derives from lack of pre-registration training and ongoing in-service education regarding the topic. The unavailability of full organ transplantation services in the province is viewed as a hindrance in the practice of organ donation. There was generally a positive attitude towards organ donation, particularly among Christian nurses. The knowledge deficit and limited practice seem not to have affected the attitudes of the participants, who supported the idea of organ donation. However, despite the positive attitudes, only 13 participants (12.0 percent) agreed to be registered as organ donors. 50 percent of participants lacked knowledge about availability and contact details of the provincial organ donation foundation, which may indicate inadequate marketing on the part of the foundation. There was no significant difference in knowledge, attitudes and practice between nurses in the private and public sectors. Rather differences were noted between the different levels of health care services, i.e. between the secondary and tertiary level. The tertiary level outperformed the secondary level institutions. It is recommended that organ donation be incorporated into the syllabi of health sciences courses both in universities and colleges, and that awareness campaigns be strengthened.
- Full Text:
Laccase production by selected bacteria species isolated from some aquatic and terrestrial milieu of the Eastern Cape : applications in wastewater treatment
- Authors: Unuofin, John Onolame
- Date: 2018
- Subjects: Laccase Sewage -- Purification -- Biological treatment Water -- Purification
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10353/9626 , vital:34810
- Description: Aromatic pollutants are a diverse group of chemicals which are continuously produced from industrialization, urbanization and sophistication in technological advancement. Pristine water source polluted by these chemicls makes the water unsafe for human consumption, and as well disrupts the trophic structure of the aquatic milieu. Physico-chemical treatment techniques employed so far have been accompanied by major drawbacks which have overriden the relative successes recorded, hence, greener, simpler and more efficient methods of pollutant transformation is imperative. The prospect of enzymatic treatment of pollutants has gradually been receiving growing attention in contemporary times due to the their environmental friendliness and production economic feasiblity. Laccase, a multicopper oxidase has heightened its appeal towards environmental and biotechnological applications due to its broad substrate specificity and its requirement of atmospheric molecular oxygen as a cosubstrate and the discharge of water as the byproduct. Hence, this present study was designed to evaluate the biotechnological potentials of laccases produced by some bacteria species from some aquatic biomes of the Eastern Cape Province, South Africa. The laccase-producing bacteria were isolated from selected environmental samples by selective enrichment using selective aromatic compounds as sole carbon source and subsequently, laccase-screening phenolic substrates. The laccase-producing bacteria were identified by molecular techniques as proteobacteria belonging to the following genera: Achromobacter, Bordetella, Citrobacter, Pseudomonas and Stenotrophomonas. Optimisation of laccase production in a submerged fermentation was by traditional and statistical methods, where four isolates (Hb9c; Achromobacter xylosoxidans HWN16, Hb16c; Bordetella bronchisepta HSO16, Berl11b2; Stenotrophomonas maltophilia BIJ16, Ie1c; Citrobacter freundii LLJ16) were evaluated for the fermentative production of laccase from lignocellulosic agroindustrial residues. Predictions from statistical optimisation showed that weakly acidic conditions (pH 5) and low agitation speed (100 rpm) were required for maximum laccase production from mandarin peelings (0.5 g/200 mL) and NaNO3 (0.25 g/200 mL) in Hb9c, maize stover (0.50 g/200 mL) and NaNO3 (0.050 g/200mL) in Berl11b2 while a lower agitation speed (50 rpm) was required for maximum laccase output from 2.0 g/200 mL maize stover and 0.050 g/200 mL KNO3 in Ie1c. However, 2.50 g/200 mL wheat bran, 0.050 g/200 mL yeast extract and 50 rpm agitiation under acidic conditions (pH3) yielded maximum laccase titres in Hb16c. Further characterisation of Hb9c and Ie1c laccase secretions portrayed their polyextremotolerant capacities. They were active at a broad range of tempertaure (0-90 degreesC); with optima at 70°C (Hb9c) and 60°C (Ie1c), pH (3-11); with optima at pH 6 (Ie1c) and pH 8 (Hb9c), respectively, and were equally thermo- and pH-stable. Their activities were either improved or left unabated by high concentrations of cations, detergents, and chloride. In addition, catalytic activities of Hb9c and Ie1c laccase secretions increased when they were preincubated with 2 – 20 percent of fluoride, a potent inhibitor. Consequently, a molecular perspective depicted the isolates to have multiple homologous laccase encoding genes. The enzymes were successfully immobilised on solid supports comprising gelatin and Na-alginate with a recovery of cca. 85 percent residual activity after 8 cycles of oprertional stability experiments. The immobilised laccases were remarkable in the decolourisation of synthetic dyes, albeit, free forms also elicited satisfactory performances. Ultimately, the application of free laccases in denim bleaching, individually or with a blend of a mediator, ABTS, showed that denim colours could be bleached without the need for chemical bleaching agents. The results obtained suggest the bacteria laccases produced from lignocellulosic wastes may serve as potent degraders of phenolic pollutants in water and, may also contribute to the bioeconomy and promote greener techniques for industrial applications.
- Full Text:
- Authors: Unuofin, John Onolame
- Date: 2018
- Subjects: Laccase Sewage -- Purification -- Biological treatment Water -- Purification
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10353/9626 , vital:34810
- Description: Aromatic pollutants are a diverse group of chemicals which are continuously produced from industrialization, urbanization and sophistication in technological advancement. Pristine water source polluted by these chemicls makes the water unsafe for human consumption, and as well disrupts the trophic structure of the aquatic milieu. Physico-chemical treatment techniques employed so far have been accompanied by major drawbacks which have overriden the relative successes recorded, hence, greener, simpler and more efficient methods of pollutant transformation is imperative. The prospect of enzymatic treatment of pollutants has gradually been receiving growing attention in contemporary times due to the their environmental friendliness and production economic feasiblity. Laccase, a multicopper oxidase has heightened its appeal towards environmental and biotechnological applications due to its broad substrate specificity and its requirement of atmospheric molecular oxygen as a cosubstrate and the discharge of water as the byproduct. Hence, this present study was designed to evaluate the biotechnological potentials of laccases produced by some bacteria species from some aquatic biomes of the Eastern Cape Province, South Africa. The laccase-producing bacteria were isolated from selected environmental samples by selective enrichment using selective aromatic compounds as sole carbon source and subsequently, laccase-screening phenolic substrates. The laccase-producing bacteria were identified by molecular techniques as proteobacteria belonging to the following genera: Achromobacter, Bordetella, Citrobacter, Pseudomonas and Stenotrophomonas. Optimisation of laccase production in a submerged fermentation was by traditional and statistical methods, where four isolates (Hb9c; Achromobacter xylosoxidans HWN16, Hb16c; Bordetella bronchisepta HSO16, Berl11b2; Stenotrophomonas maltophilia BIJ16, Ie1c; Citrobacter freundii LLJ16) were evaluated for the fermentative production of laccase from lignocellulosic agroindustrial residues. Predictions from statistical optimisation showed that weakly acidic conditions (pH 5) and low agitation speed (100 rpm) were required for maximum laccase production from mandarin peelings (0.5 g/200 mL) and NaNO3 (0.25 g/200 mL) in Hb9c, maize stover (0.50 g/200 mL) and NaNO3 (0.050 g/200mL) in Berl11b2 while a lower agitation speed (50 rpm) was required for maximum laccase output from 2.0 g/200 mL maize stover and 0.050 g/200 mL KNO3 in Ie1c. However, 2.50 g/200 mL wheat bran, 0.050 g/200 mL yeast extract and 50 rpm agitiation under acidic conditions (pH3) yielded maximum laccase titres in Hb16c. Further characterisation of Hb9c and Ie1c laccase secretions portrayed their polyextremotolerant capacities. They were active at a broad range of tempertaure (0-90 degreesC); with optima at 70°C (Hb9c) and 60°C (Ie1c), pH (3-11); with optima at pH 6 (Ie1c) and pH 8 (Hb9c), respectively, and were equally thermo- and pH-stable. Their activities were either improved or left unabated by high concentrations of cations, detergents, and chloride. In addition, catalytic activities of Hb9c and Ie1c laccase secretions increased when they were preincubated with 2 – 20 percent of fluoride, a potent inhibitor. Consequently, a molecular perspective depicted the isolates to have multiple homologous laccase encoding genes. The enzymes were successfully immobilised on solid supports comprising gelatin and Na-alginate with a recovery of cca. 85 percent residual activity after 8 cycles of oprertional stability experiments. The immobilised laccases were remarkable in the decolourisation of synthetic dyes, albeit, free forms also elicited satisfactory performances. Ultimately, the application of free laccases in denim bleaching, individually or with a blend of a mediator, ABTS, showed that denim colours could be bleached without the need for chemical bleaching agents. The results obtained suggest the bacteria laccases produced from lignocellulosic wastes may serve as potent degraders of phenolic pollutants in water and, may also contribute to the bioeconomy and promote greener techniques for industrial applications.
- Full Text:
Land reform and livelihood among subsistence farmers in Great Kei local municipality, South Africa
- Authors: Bani, Tida Witness
- Date: 2018
- Subjects: Land reform Subsistence farming Sustainable development
- Language: English
- Type: Thesis , Masters , MSoc
- Identifier: http://hdl.handle.net/10353/12870 , vital:39386
- Description: Land reform, as enshrined in the constitution of the Republic of South Africa, aims to foster conditions which enable citizens to gain access to land on equitable basis. Section 25(6) of the constitution states that ‘a person or community whose tenure of land is legally insecure as a result of past racially discriminatory laws or practice is entitled, to the extent provided by an Act of Parliament, either to tenure, which is legally secure, or comparable redress. In addition, section 25(7) states that a person or community dispossessed of property after 19 June 1913 as a result of past racially discriminatory laws or practices is entitled to the extent provided by an Act of Parliament, either to restitution of that property or to equitable redress. Within this context, this study assessed the impact of land reform on the livelihoods of subsistence farmers in Great Kei Local Municipality. The study adopted a qualitative approach in order to get an in-depth understanding of the farming activities of land reform beneficiaries, the contribution of farming activities to agro-based sustainable livelihoods and the general challenges faced by subsistence farmers. The sample comprised of thirty (30) farmers, two focus group discussions which comprised of six (6) farmers each and five key informants from the Department of Rural development and Agrarian Reform, a councillor and a community leader from the local municipality. The study found that most farmers who were allocated land were from different disadvantaged backgrounds. Many of the farmers came from farming backgrounds hence they had the requisite skills and knowledge. Those with relatively limited knowledge and skills were technically supported and subsequently improved their production and livelihoods. Those farmers that were trained in various farming aspects did well as they improved production. However, it was found that those farmers that did not receive training and adequate support yielded negative results in both crop and animal production. Drought has been another major contributor in the decline of the production. It was also found that the post settlement grants, such as Comprehensive Agricultural Support Programme and Recapitalization and Agricultural Development, are not enough and regular for infrastructural development on the farms.
- Full Text:
- Authors: Bani, Tida Witness
- Date: 2018
- Subjects: Land reform Subsistence farming Sustainable development
- Language: English
- Type: Thesis , Masters , MSoc
- Identifier: http://hdl.handle.net/10353/12870 , vital:39386
- Description: Land reform, as enshrined in the constitution of the Republic of South Africa, aims to foster conditions which enable citizens to gain access to land on equitable basis. Section 25(6) of the constitution states that ‘a person or community whose tenure of land is legally insecure as a result of past racially discriminatory laws or practice is entitled, to the extent provided by an Act of Parliament, either to tenure, which is legally secure, or comparable redress. In addition, section 25(7) states that a person or community dispossessed of property after 19 June 1913 as a result of past racially discriminatory laws or practices is entitled to the extent provided by an Act of Parliament, either to restitution of that property or to equitable redress. Within this context, this study assessed the impact of land reform on the livelihoods of subsistence farmers in Great Kei Local Municipality. The study adopted a qualitative approach in order to get an in-depth understanding of the farming activities of land reform beneficiaries, the contribution of farming activities to agro-based sustainable livelihoods and the general challenges faced by subsistence farmers. The sample comprised of thirty (30) farmers, two focus group discussions which comprised of six (6) farmers each and five key informants from the Department of Rural development and Agrarian Reform, a councillor and a community leader from the local municipality. The study found that most farmers who were allocated land were from different disadvantaged backgrounds. Many of the farmers came from farming backgrounds hence they had the requisite skills and knowledge. Those with relatively limited knowledge and skills were technically supported and subsequently improved their production and livelihoods. Those farmers that were trained in various farming aspects did well as they improved production. However, it was found that those farmers that did not receive training and adequate support yielded negative results in both crop and animal production. Drought has been another major contributor in the decline of the production. It was also found that the post settlement grants, such as Comprehensive Agricultural Support Programme and Recapitalization and Agricultural Development, are not enough and regular for infrastructural development on the farms.
- Full Text:
Livelihood and unemployment transitions : drivers and effects on welfare in Nigeria
- Authors: Fashogbon, Ayodele Emmanuel
- Date: 2018
- Subjects: Social service -- Nigeria Social problems -- Nigeria Nigeria -- Social conditions
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10353/9176 , vital:34302
- Description: Hunger, unemployment and social crisis are increasing in Nigeria. Statistics supports it that most of the able-bodied youth, both male and female, are not in employment, school or training in Nigeria while poverty rate is as high as a decade ago. A number of research efforts have looked at the micro and macroeconomic paths of the problem with less attention on dynamism of unemployment vis-a-vis the labour market transition, hence this study. This study investigates dynamism of labour market by assessing pattern and transition in labour market and out of labour, as well as determining the effect size of households head’s unemployment on household welfare. To achieve this objective, the General Household Survey (GHS) panel data for 2010/211 and 2012/2013 for Nigeria was used coupled with panel regression analyses to identify determinants of transitions from unemployment to productive sectors, and determinants of transition from productive sectors to unemployment, as well as effect of unemployment on household welfare while controlling for potential endogeneity in unemployment. Across the productive sectors and between 2010 and 2013, the descriptive results of transition show that workforce in the services sector (68 percent) are the most persistent, followed by agriculture (59 percent) and least is mining and manufacturing (44 percent). Transition from nonemployment occurred the most to services sector (22 percent), followed by out-of-labour force (18 percent), agriculture (15 percent) and least to mining and manufacturing (5 percent). Further, the results show that there are both locations and gender differences in the extent of transitions in the labour market. The econometric analyses reveal that job search characteristics, age, educational levels, gender, family ties, locations and time significantly determine transition from unemployment to productive sectors (agriculture or non-agriculture). Likewise, job characteristics, demographic variables, social network, location and time significantly determine switch from one productive sector to another or to unemployment. Moreover, it explicitly reveals as well from the analysis that household head month of unemployment reduces household total consumption and food consumption by 6.4 percent with gender and sectorial differences. The study recommends a combined and swift intervention on three sides: one, there is a need to stimulate education either through scholarship, or adult education from both public and private sectors; two, need to create jobs for youth to reduce potential social and economic crisis; and lastly there is a need to provide social support either in terms of public workfare, skills for jobs and unemployment benefits.
- Full Text:
- Authors: Fashogbon, Ayodele Emmanuel
- Date: 2018
- Subjects: Social service -- Nigeria Social problems -- Nigeria Nigeria -- Social conditions
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10353/9176 , vital:34302
- Description: Hunger, unemployment and social crisis are increasing in Nigeria. Statistics supports it that most of the able-bodied youth, both male and female, are not in employment, school or training in Nigeria while poverty rate is as high as a decade ago. A number of research efforts have looked at the micro and macroeconomic paths of the problem with less attention on dynamism of unemployment vis-a-vis the labour market transition, hence this study. This study investigates dynamism of labour market by assessing pattern and transition in labour market and out of labour, as well as determining the effect size of households head’s unemployment on household welfare. To achieve this objective, the General Household Survey (GHS) panel data for 2010/211 and 2012/2013 for Nigeria was used coupled with panel regression analyses to identify determinants of transitions from unemployment to productive sectors, and determinants of transition from productive sectors to unemployment, as well as effect of unemployment on household welfare while controlling for potential endogeneity in unemployment. Across the productive sectors and between 2010 and 2013, the descriptive results of transition show that workforce in the services sector (68 percent) are the most persistent, followed by agriculture (59 percent) and least is mining and manufacturing (44 percent). Transition from nonemployment occurred the most to services sector (22 percent), followed by out-of-labour force (18 percent), agriculture (15 percent) and least to mining and manufacturing (5 percent). Further, the results show that there are both locations and gender differences in the extent of transitions in the labour market. The econometric analyses reveal that job search characteristics, age, educational levels, gender, family ties, locations and time significantly determine transition from unemployment to productive sectors (agriculture or non-agriculture). Likewise, job characteristics, demographic variables, social network, location and time significantly determine switch from one productive sector to another or to unemployment. Moreover, it explicitly reveals as well from the analysis that household head month of unemployment reduces household total consumption and food consumption by 6.4 percent with gender and sectorial differences. The study recommends a combined and swift intervention on three sides: one, there is a need to stimulate education either through scholarship, or adult education from both public and private sectors; two, need to create jobs for youth to reduce potential social and economic crisis; and lastly there is a need to provide social support either in terms of public workfare, skills for jobs and unemployment benefits.
- Full Text:
Livelihood strategies determinants and ranking across times : evidence from Eastern Cape
- Authors: Sikhunyana, Zintle
- Date: 2018
- Subjects: Households Rural development
- Language: English
- Type: Thesis , Masters , MCom
- Identifier: http://hdl.handle.net/10353/15079 , vital:40161
- Description: The existing literature has acknowledged that analysis of livelihood strategies is more important for rural development policy and also that livelihood strategies are at the centre of development. Persistent challenges of poverty, unemployment and inequality have called for a need to understand livelihood patterns of poor households as to effectively address these socio-economic issues in rural areas. The study aimed to identify determinants of livelihood strategies and to identify livelihood strategies that are superior to others in the rural Eastern Cape province of South Africa for the years 2002-2014. The study used multinomial logistic regression to identify the determinants of livelihood strategies. The findings showed that: age of household head, gender of household head, dwelling type, household size, number of economically active members, size of land that the household uses, and access to electricity statistically determine livelihood strategies of the poor in rural Eastern Cape. With regards to ranking, the study used dominance tests such as the Kruskal-Wallis test and also used one way between groups ANOVA with the post hoc test for additional information. The results from the Kruskal-Wallis test showed that remittances are superior livelihood strategies, and the results from one-way between groups ANOVA with post hoc showed that sales of farm products and services dominate other strategies. Restrictions on land conservation, and promotion of sustainable development have constrained rural households from freely using land in pursuit of their goals. The government needs to reconcile the policy amendments for land use. Furthermore, social grants alone are not sufficient to meet the needs of the poor; there should be alternative strategies on how to take the poor out of poverty.
- Full Text:
- Authors: Sikhunyana, Zintle
- Date: 2018
- Subjects: Households Rural development
- Language: English
- Type: Thesis , Masters , MCom
- Identifier: http://hdl.handle.net/10353/15079 , vital:40161
- Description: The existing literature has acknowledged that analysis of livelihood strategies is more important for rural development policy and also that livelihood strategies are at the centre of development. Persistent challenges of poverty, unemployment and inequality have called for a need to understand livelihood patterns of poor households as to effectively address these socio-economic issues in rural areas. The study aimed to identify determinants of livelihood strategies and to identify livelihood strategies that are superior to others in the rural Eastern Cape province of South Africa for the years 2002-2014. The study used multinomial logistic regression to identify the determinants of livelihood strategies. The findings showed that: age of household head, gender of household head, dwelling type, household size, number of economically active members, size of land that the household uses, and access to electricity statistically determine livelihood strategies of the poor in rural Eastern Cape. With regards to ranking, the study used dominance tests such as the Kruskal-Wallis test and also used one way between groups ANOVA with the post hoc test for additional information. The results from the Kruskal-Wallis test showed that remittances are superior livelihood strategies, and the results from one-way between groups ANOVA with post hoc showed that sales of farm products and services dominate other strategies. Restrictions on land conservation, and promotion of sustainable development have constrained rural households from freely using land in pursuit of their goals. The government needs to reconcile the policy amendments for land use. Furthermore, social grants alone are not sufficient to meet the needs of the poor; there should be alternative strategies on how to take the poor out of poverty.
- Full Text:
Macro-prudential banking regulation, interest rate spread and the conduct of monetary policy in South Africa
- Shayanewako,Varaidzo Batsirai
- Authors: Shayanewako,Varaidzo Batsirai
- Date: 2018
- Subjects: Monetary policy Economic stabilization
- Language: English
- Type: Thesis , Doctoral , D.Com
- Identifier: http://hdl.handle.net/10353/14093 , vital:39843
- Description: The joint implementation of macro-prudential regulatory and monetary policies is necessary for modern banking systems not only to achieve optimal interest rate spread but to cushion the economy against the catastrophic effects of systemic risk. Therefore, the understanding of the interconnection between macroprudential regulation, interest rate spread and monetary policy, supported with empirical evidence, enables economies to build health and sound financial systems and achieve desirable growth rates. Thus, this study utilises the Vector Error Correction (VEC) mechanism to empirically investigate the interconnection between macro-prudential regulation, interest rate spread and monetary policy in South Africa. Quarterly time series data for the period from 1994 to 2016 was employed with the New Keynesian Dynamic Stochastic General Equilibrium (NKDSGE) model as the framework for analysis. The findings of this study suggest that a negative relationship exists between interest rate spread, macro-prudential regulation and monetary policy in South Africa. Moreover, the systems equation provided evidence that a short run causality running from macroprudential regulation to monetary policy is prevalent in South Africa. The empirical model of the study was found to be desirable as evidence of no serial correlation, no ARCH effect and non-normality in residuals was detected. Evidence from this study further suggest that interest rate spread has a dampening effect on monetary policy, but in the long-run this effect seems reversible in South Africa. As a result, this study recommends that caution should be taken on the appropriate selection of measures of macro-prudential regulation and its tool-kit as it can be used to disguise the symptoms of a lax monetary policy. This implies that the South African Reserve Bank (SARB) should sternly supervise and regulate the extension of credit by commercial banks in line with its inflation targeting monetary policy rule in order to achieve financial stability and ensure optimal interest rate spread that can stimulate the economy to growth.
- Full Text:
- Authors: Shayanewako,Varaidzo Batsirai
- Date: 2018
- Subjects: Monetary policy Economic stabilization
- Language: English
- Type: Thesis , Doctoral , D.Com
- Identifier: http://hdl.handle.net/10353/14093 , vital:39843
- Description: The joint implementation of macro-prudential regulatory and monetary policies is necessary for modern banking systems not only to achieve optimal interest rate spread but to cushion the economy against the catastrophic effects of systemic risk. Therefore, the understanding of the interconnection between macroprudential regulation, interest rate spread and monetary policy, supported with empirical evidence, enables economies to build health and sound financial systems and achieve desirable growth rates. Thus, this study utilises the Vector Error Correction (VEC) mechanism to empirically investigate the interconnection between macro-prudential regulation, interest rate spread and monetary policy in South Africa. Quarterly time series data for the period from 1994 to 2016 was employed with the New Keynesian Dynamic Stochastic General Equilibrium (NKDSGE) model as the framework for analysis. The findings of this study suggest that a negative relationship exists between interest rate spread, macro-prudential regulation and monetary policy in South Africa. Moreover, the systems equation provided evidence that a short run causality running from macroprudential regulation to monetary policy is prevalent in South Africa. The empirical model of the study was found to be desirable as evidence of no serial correlation, no ARCH effect and non-normality in residuals was detected. Evidence from this study further suggest that interest rate spread has a dampening effect on monetary policy, but in the long-run this effect seems reversible in South Africa. As a result, this study recommends that caution should be taken on the appropriate selection of measures of macro-prudential regulation and its tool-kit as it can be used to disguise the symptoms of a lax monetary policy. This implies that the South African Reserve Bank (SARB) should sternly supervise and regulate the extension of credit by commercial banks in line with its inflation targeting monetary policy rule in order to achieve financial stability and ensure optimal interest rate spread that can stimulate the economy to growth.
- Full Text:
Media portrayal of gender based violence: a perception study in Uitenhage, Eastern Cape South Africa 2
- Oladimeji Olawumi Kate https://orcid.org/0000-0002-6577-0140
- Authors: Oladimeji Olawumi Kate https://orcid.org/0000-0002-6577-0140
- Date: 2018
- Subjects: Abused women , Mass media -- Eastern Cape -- South Africa , Mass media criticism -- Eastern Cape -- South Africa
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/19476 , vital:43131
- Description: Gender based violence (GBV) has become a global menace. The prevalence of this violence against women and children in South Africa reveals that the country is plagued by this horror called GBV. According to the South African police crime statistics, with regards to sexual offences, the SAPS reported 64 419 (2012), 66 197 (2014), 62 226 (2013) and 53 617 (2015) cases. A government survey conducted revealed that men were the main perpetrators of this violence. For example, 76 percent of men in Gauteng, 48 percent in Limpopo and 41 percent in KwaZulu-Natal admitted to perpetrating GBV. These alarming statistics calls for the attention of the media. The media is known to be a powerful tool of influence, however in the process, the media has been criticised to portray violence in ways which can have varying effect on the behaviour of those exposed to it. This study was therefore conducted to investigate the perception of the residents of Uitenhage on the media‘s portrayal of gender based violence against women and children. The mixed method of research was used to obtain data from the participants and respondents which were residents of Uitenhage, in the Eastern Cape, South Africa. Non-probability sampling in the form of purposive sampling was used to select the focus group participants while probability sampling in the form of simple random probability sampling was used to select the respondents with whom the questionnaires were administered to. Quantitative data analysis for questionnaires was done using descriptive statistics and findings were presented in form of tables and pie charts while qualitative data analysis for the focus group discussions was done using thematic analysis. A final sample of 257 valid responses were analysed, representing 80 percent response rate, three focus group discussions were also analysed. The study revealed that all the participants and respondents are exposed to more than one medium of mass communication, however participants and respondents opined that the media has both positive and negative effect on different individuals. The study also demonstrated that there is an association between people‘s exposure to the media and their violent behaviour which is attributed to the messages conveyed by the media. The study recommends amongst others that the media should take a more proactive approach to the issue of VAW. , Thesis (PhD) (Communication) -- University of Fort Hare, 2018
- Full Text:
- Authors: Oladimeji Olawumi Kate https://orcid.org/0000-0002-6577-0140
- Date: 2018
- Subjects: Abused women , Mass media -- Eastern Cape -- South Africa , Mass media criticism -- Eastern Cape -- South Africa
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/19476 , vital:43131
- Description: Gender based violence (GBV) has become a global menace. The prevalence of this violence against women and children in South Africa reveals that the country is plagued by this horror called GBV. According to the South African police crime statistics, with regards to sexual offences, the SAPS reported 64 419 (2012), 66 197 (2014), 62 226 (2013) and 53 617 (2015) cases. A government survey conducted revealed that men were the main perpetrators of this violence. For example, 76 percent of men in Gauteng, 48 percent in Limpopo and 41 percent in KwaZulu-Natal admitted to perpetrating GBV. These alarming statistics calls for the attention of the media. The media is known to be a powerful tool of influence, however in the process, the media has been criticised to portray violence in ways which can have varying effect on the behaviour of those exposed to it. This study was therefore conducted to investigate the perception of the residents of Uitenhage on the media‘s portrayal of gender based violence against women and children. The mixed method of research was used to obtain data from the participants and respondents which were residents of Uitenhage, in the Eastern Cape, South Africa. Non-probability sampling in the form of purposive sampling was used to select the focus group participants while probability sampling in the form of simple random probability sampling was used to select the respondents with whom the questionnaires were administered to. Quantitative data analysis for questionnaires was done using descriptive statistics and findings were presented in form of tables and pie charts while qualitative data analysis for the focus group discussions was done using thematic analysis. A final sample of 257 valid responses were analysed, representing 80 percent response rate, three focus group discussions were also analysed. The study revealed that all the participants and respondents are exposed to more than one medium of mass communication, however participants and respondents opined that the media has both positive and negative effect on different individuals. The study also demonstrated that there is an association between people‘s exposure to the media and their violent behaviour which is attributed to the messages conveyed by the media. The study recommends amongst others that the media should take a more proactive approach to the issue of VAW. , Thesis (PhD) (Communication) -- University of Fort Hare, 2018
- Full Text:
Metabolic Systems Biology: Uncovering operations of cell and metabolism
- Authors: Marange , Israel Yeukai
- Date: 2018
- Subjects: Systems biology
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/19120 , vital:39881
- Description: The aim of this work is to analyse the technique for formulating cellular metabolism in living organisms. It also focuses on developing models of cellular metabolic systems. A sequence of biochemical reactions termed metabolic branched pathways that occur inside a cell, are of particular interest.Mathematical modelling is used as a tool to examine and analyse the metabolism of viral dynamics for the ends of this research. Dynamic mathematical models provide an important means with which to investigate the cell cycle control system. The derivation of the ordinary differential equations (ODEs) is useful in describing and analysing the metabolic network structures of branched flux pathways, as well as enzyme-substrate,reciprocal-action networks which control the cell cycle. Different general and specific factors affecting metabolic rate are identified and explained
- Full Text:
- Authors: Marange , Israel Yeukai
- Date: 2018
- Subjects: Systems biology
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/19120 , vital:39881
- Description: The aim of this work is to analyse the technique for formulating cellular metabolism in living organisms. It also focuses on developing models of cellular metabolic systems. A sequence of biochemical reactions termed metabolic branched pathways that occur inside a cell, are of particular interest.Mathematical modelling is used as a tool to examine and analyse the metabolism of viral dynamics for the ends of this research. Dynamic mathematical models provide an important means with which to investigate the cell cycle control system. The derivation of the ordinary differential equations (ODEs) is useful in describing and analysing the metabolic network structures of branched flux pathways, as well as enzyme-substrate,reciprocal-action networks which control the cell cycle. Different general and specific factors affecting metabolic rate are identified and explained
- Full Text:
Molecular characterization of streptococcus agalactiae isolated from pregnant women in the Eastern Cape, South Africa and Windhoek, Namibia and antibacterial activities of some medicinal plant extracts on the isolates
- Authors: Mukesi, Munyaradzi
- Date: 2018
- Subjects: Streptococcus Medicinal plants Pregnant women
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10353/13034 , vital:39437
- Description: Streptococcus agalactiae (S. agalactiae) also known as group B Streptococcus (GBS) is one of the leading causes of bacterial morbidity and mortality among neonates worldwide. It is the cause of invasive Early Onset Disease (EOD), which occurs in the first 7 days of life and characterised by sepsis, pneumonia and meningitis and Late Onset Disease (LOD) occurring between 7 and 89 days of life. Late onset disease is characterised by meningitis and long term neurological sequelae such as cerebral palsy, hearing impairment and cognitive challenges. S. agalactiae does not only infect neonates, it also infects the elderly, immunocompromised individuals and pregnant and non-pregnant women, causing invasive disease. In the world, 10-40 percent of healthy women are rectally or vaginally colonised with GBS and they face the risk of passing it to their babies during the process of childbirth. During parturition, a GBS colonized pregnant woman transfers the bacterium to her new-born as the baby passes through the ruptured membrane, thus infecting the child. However, GBS has been reported to be transferred even without rupture of membranes. Once it infects the membranes, it is transferred into the amniotic fluid and subsequently infects the baby. It can be aspirated into the lungs causing pneumonia or it can infect the blood stream and disseminated round the body causing septicaemia, meningitis and other infections. Once in the neonate’s body, the bacteria is able to evade the immune system as the host immune system is not yet fully developed. Bacterial evasion of the immune system is enhanced by its various virulence factors which are deployed to help it escape the immune system. These include the polysaccharide capsule, haemolysin and the release of complement inactivating factors such C5a peptidase. The World Health Organisation (WHO) (2010) recommends universal screening of pregnant women to identify those colonised and who are at risk of passing the bacterium to their babies during birth. WHO also recommends identification of at risk women and providing Intrapartum Antibiotic Prophylaxis (IAP) using penicillin. However, problems arise in penicillin allergic women and while alternatives for IAP include erythromycin and clindamycin, there is increasing resistance to these drugs thereby limiting therapeutic options. Antimicrobial susceptibility testing is also not always possible in most resource constrained countries due to poor infrastructure, limited access to health care and the logistical problems in implementing the WHO guidelines. Alternative therapeutic options to GBS infection include developing new and potent antibiotics, development of a vaccine, use of medicinal plants and the use of bacteriophage therapy. While these look like better alternatives there is massive scientific work to be carried out to ensure proper characterisation and efficiency of such alternatives. This process should be followed by in vitro diagnostic testing, experiments with animal models and clinical trials. The problems encountered during vaccine development to curtail GBS infection are compounded by the multiplicity of S. agalactiae capsular types which vary in different geographic locations. Medicinal plants are a cheap and convenient option since they are widely used in communities but the phytochemical components of the plants have to be identified and subjected to in vitro testing to evaluate their therapeutic efficacy as antimicrobial agents. This study therefore sought to isolate GBS from pregnant women between 35 and 37 weeks gestation in Windhoek (Namibia) and the Eastern Cape (South Africa), to determine the prevalence of GBS colonisation in the vagina and rectum of the pregnant women, characterise the isolates by molecular techniques, determine the antimicrobial resistance profiles and genes of the isolates and explore the efficacies of medicinal plant extracts as possible candidates for therapeutic options.
- Full Text:
- Authors: Mukesi, Munyaradzi
- Date: 2018
- Subjects: Streptococcus Medicinal plants Pregnant women
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10353/13034 , vital:39437
- Description: Streptococcus agalactiae (S. agalactiae) also known as group B Streptococcus (GBS) is one of the leading causes of bacterial morbidity and mortality among neonates worldwide. It is the cause of invasive Early Onset Disease (EOD), which occurs in the first 7 days of life and characterised by sepsis, pneumonia and meningitis and Late Onset Disease (LOD) occurring between 7 and 89 days of life. Late onset disease is characterised by meningitis and long term neurological sequelae such as cerebral palsy, hearing impairment and cognitive challenges. S. agalactiae does not only infect neonates, it also infects the elderly, immunocompromised individuals and pregnant and non-pregnant women, causing invasive disease. In the world, 10-40 percent of healthy women are rectally or vaginally colonised with GBS and they face the risk of passing it to their babies during the process of childbirth. During parturition, a GBS colonized pregnant woman transfers the bacterium to her new-born as the baby passes through the ruptured membrane, thus infecting the child. However, GBS has been reported to be transferred even without rupture of membranes. Once it infects the membranes, it is transferred into the amniotic fluid and subsequently infects the baby. It can be aspirated into the lungs causing pneumonia or it can infect the blood stream and disseminated round the body causing septicaemia, meningitis and other infections. Once in the neonate’s body, the bacteria is able to evade the immune system as the host immune system is not yet fully developed. Bacterial evasion of the immune system is enhanced by its various virulence factors which are deployed to help it escape the immune system. These include the polysaccharide capsule, haemolysin and the release of complement inactivating factors such C5a peptidase. The World Health Organisation (WHO) (2010) recommends universal screening of pregnant women to identify those colonised and who are at risk of passing the bacterium to their babies during birth. WHO also recommends identification of at risk women and providing Intrapartum Antibiotic Prophylaxis (IAP) using penicillin. However, problems arise in penicillin allergic women and while alternatives for IAP include erythromycin and clindamycin, there is increasing resistance to these drugs thereby limiting therapeutic options. Antimicrobial susceptibility testing is also not always possible in most resource constrained countries due to poor infrastructure, limited access to health care and the logistical problems in implementing the WHO guidelines. Alternative therapeutic options to GBS infection include developing new and potent antibiotics, development of a vaccine, use of medicinal plants and the use of bacteriophage therapy. While these look like better alternatives there is massive scientific work to be carried out to ensure proper characterisation and efficiency of such alternatives. This process should be followed by in vitro diagnostic testing, experiments with animal models and clinical trials. The problems encountered during vaccine development to curtail GBS infection are compounded by the multiplicity of S. agalactiae capsular types which vary in different geographic locations. Medicinal plants are a cheap and convenient option since they are widely used in communities but the phytochemical components of the plants have to be identified and subjected to in vitro testing to evaluate their therapeutic efficacy as antimicrobial agents. This study therefore sought to isolate GBS from pregnant women between 35 and 37 weeks gestation in Windhoek (Namibia) and the Eastern Cape (South Africa), to determine the prevalence of GBS colonisation in the vagina and rectum of the pregnant women, characterise the isolates by molecular techniques, determine the antimicrobial resistance profiles and genes of the isolates and explore the efficacies of medicinal plant extracts as possible candidates for therapeutic options.
- Full Text:
Molecular epidemiology of vibrio pathogens in selected surface waters and treated final effluents of wastewater treatment plants in Chris Hani District Municipality in the Eastern Cape Province, South Africa
- Authors: Ayodeji, Onsula Charles
- Date: 2018
- Subjects: Sewage disposal plants Sewage -- Purification Water -- Purification -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Doctoral , Microbiology
- Identifier: http://hdl.handle.net/10353/10464 , vital:35478
- Description: Access to clean and safe water is essential for the survival of human beings. Nevertheless pollution from wastewater treatment plants (WWTPs) effluents often impacts the microbiological qualities of surface waters. Availability of freshwater resources is indispensable for preventing waterborne diseases. The current study evaluated the physicochemical properties and abundance of Vibrio species in selected rivers and WWTPs in the Eastern Cape Province of South Africa, over a one year sampling regime (December, 2016 to November, 2017). Water samples were collected monthly at designated locations along the banks of two rivers, treated effluents from 500 m upstream and downstream discharge points of two WWTPs and examined for physicochemical qualities, prevalence of Vibrio pathogens and their antibiogram profiles using both cultural and molecular techniques. The findings reveal the qualities of Tsomo and Great Fish river qualities with respect to electrical conductivity (EC) (137.75 – 377.69 mg/l), total suspended solids (TSS) (52.00 – 725.19 mg/l) and temperature (12.7 – 23 oC) and the treated effluents of WW-AD and WW-CR WWTPs with respect to EC (525.73 – 1071.89 mg/l), free chlorine (0.00 mg/l – 0.68 mg/l), temperature (12.4 – 28.8 oC), TSS (14.67 – 276.44 mg/l) exceeds the permissible limits set for effluent discharged to freshwater by South Africa guidelines. Other physicochemical qualities such as turbidity (63.11 – 797.17 NTU), BOD (3.30 – 5.26 mg/l) and TDS (68.88 – 148.14 mg/l) for Tsomo and Great Fish river and turbidity (20.33 – 310 NTU), biological oxygen demand (BOD) (1.28 – 4.96 mg/l), and total dissolve solid (TDS) (262.89 – 534.89 mg/l) for WWTPs WW-AD and WW-CR did not comply with WHO and EU standards as no regulation is set for them in the South African guidelines for domestic water usage. Statistical analysis revealed that pH, electrical conductivities, dissolved oxygen and total dissolved solid were significantly different whereas temperature did not differ significantly with respect to the four seasons (P < 0.05). The Vibrio densities for Great Fish river ranged between 0 and 3.29 log10 CFU/ml with the highest obtained in the spring. The Tsomo River Vibrio densities varied between 0 to 3.56 log10 CFU/ml and the maximum densities recorded during summer. The presumptive Vibrio densities in WW-AD and WW-CR WWTPs ranged from 0 to 3.67 log10 CFU/ml and 0 to 4.42 log10 CFU/ml with autumn and spring having the highest loads respectively. Molecular identification of the presumptive Vibrio species revealed 424 positive for the Vibrio genus. Of these, 21.69 percent, 11.79 percent, 8.25 percent and 2.12 percent were confirmed as V. cholerae, V. mimicus, V. parahaemolyticus and V. fluvialis respectively. The presence of potentially Vibrio pathogens in the rivers and treated effluents suggests potential public health threat to the communities relying on receiving watersheds where the effluents are discharged. Results of antibiotic sensitivity testing revealed high sensitivities against Ofloxacin (85.54 percent), Cefuroxime (81.93 percent), and Ciprofloxacin (74.70 percent), whereas resistance against other antibiotics follow this order: Imipenem (42.17 percent), Amoxicillin-Clavulanic Acid (54.17 percent), Ampicillin (63.54 percent), Nitrofurantoin (71.79 percent) and Polymyxin B (97.44 percent). Surprisingly, the resistance of Vibrio species against the known effective Carbapenems was relatively high (Meropenem (38.54 percent) and Imipenem (58.88 percent). Multiple antibiotic resistance phenotypes (MARP) of the isolates were resistant to two or more antibiotics whereas the calculated multiple antibiotic resistance index (MARI) ranged from 0.2 to 0.7. The observed high multiple antibiotics index suggest the recovered Vibrio pathogens are of high antimicrobial usage origin. Increase in antimicrobial resistance profiles towards conventionally used antibiotics as indicated in this study calls for adequate sanitation facilities and proper surveillance programs towards monitoring of antimicrobial resistance determinants in wastewater treatment effluents and receiving watersheds. This will in turn enhance early detection of resistant strains of public health importance, and supports the prompt notification and investigation of outbreaks. It further advocates the need for constant monitoring programme by the relevant regulatory agencies to ensure total compliance of the wastewater treatment facilities to the stipulated standards.
- Full Text:
- Authors: Ayodeji, Onsula Charles
- Date: 2018
- Subjects: Sewage disposal plants Sewage -- Purification Water -- Purification -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Doctoral , Microbiology
- Identifier: http://hdl.handle.net/10353/10464 , vital:35478
- Description: Access to clean and safe water is essential for the survival of human beings. Nevertheless pollution from wastewater treatment plants (WWTPs) effluents often impacts the microbiological qualities of surface waters. Availability of freshwater resources is indispensable for preventing waterborne diseases. The current study evaluated the physicochemical properties and abundance of Vibrio species in selected rivers and WWTPs in the Eastern Cape Province of South Africa, over a one year sampling regime (December, 2016 to November, 2017). Water samples were collected monthly at designated locations along the banks of two rivers, treated effluents from 500 m upstream and downstream discharge points of two WWTPs and examined for physicochemical qualities, prevalence of Vibrio pathogens and their antibiogram profiles using both cultural and molecular techniques. The findings reveal the qualities of Tsomo and Great Fish river qualities with respect to electrical conductivity (EC) (137.75 – 377.69 mg/l), total suspended solids (TSS) (52.00 – 725.19 mg/l) and temperature (12.7 – 23 oC) and the treated effluents of WW-AD and WW-CR WWTPs with respect to EC (525.73 – 1071.89 mg/l), free chlorine (0.00 mg/l – 0.68 mg/l), temperature (12.4 – 28.8 oC), TSS (14.67 – 276.44 mg/l) exceeds the permissible limits set for effluent discharged to freshwater by South Africa guidelines. Other physicochemical qualities such as turbidity (63.11 – 797.17 NTU), BOD (3.30 – 5.26 mg/l) and TDS (68.88 – 148.14 mg/l) for Tsomo and Great Fish river and turbidity (20.33 – 310 NTU), biological oxygen demand (BOD) (1.28 – 4.96 mg/l), and total dissolve solid (TDS) (262.89 – 534.89 mg/l) for WWTPs WW-AD and WW-CR did not comply with WHO and EU standards as no regulation is set for them in the South African guidelines for domestic water usage. Statistical analysis revealed that pH, electrical conductivities, dissolved oxygen and total dissolved solid were significantly different whereas temperature did not differ significantly with respect to the four seasons (P < 0.05). The Vibrio densities for Great Fish river ranged between 0 and 3.29 log10 CFU/ml with the highest obtained in the spring. The Tsomo River Vibrio densities varied between 0 to 3.56 log10 CFU/ml and the maximum densities recorded during summer. The presumptive Vibrio densities in WW-AD and WW-CR WWTPs ranged from 0 to 3.67 log10 CFU/ml and 0 to 4.42 log10 CFU/ml with autumn and spring having the highest loads respectively. Molecular identification of the presumptive Vibrio species revealed 424 positive for the Vibrio genus. Of these, 21.69 percent, 11.79 percent, 8.25 percent and 2.12 percent were confirmed as V. cholerae, V. mimicus, V. parahaemolyticus and V. fluvialis respectively. The presence of potentially Vibrio pathogens in the rivers and treated effluents suggests potential public health threat to the communities relying on receiving watersheds where the effluents are discharged. Results of antibiotic sensitivity testing revealed high sensitivities against Ofloxacin (85.54 percent), Cefuroxime (81.93 percent), and Ciprofloxacin (74.70 percent), whereas resistance against other antibiotics follow this order: Imipenem (42.17 percent), Amoxicillin-Clavulanic Acid (54.17 percent), Ampicillin (63.54 percent), Nitrofurantoin (71.79 percent) and Polymyxin B (97.44 percent). Surprisingly, the resistance of Vibrio species against the known effective Carbapenems was relatively high (Meropenem (38.54 percent) and Imipenem (58.88 percent). Multiple antibiotic resistance phenotypes (MARP) of the isolates were resistant to two or more antibiotics whereas the calculated multiple antibiotic resistance index (MARI) ranged from 0.2 to 0.7. The observed high multiple antibiotics index suggest the recovered Vibrio pathogens are of high antimicrobial usage origin. Increase in antimicrobial resistance profiles towards conventionally used antibiotics as indicated in this study calls for adequate sanitation facilities and proper surveillance programs towards monitoring of antimicrobial resistance determinants in wastewater treatment effluents and receiving watersheds. This will in turn enhance early detection of resistant strains of public health importance, and supports the prompt notification and investigation of outbreaks. It further advocates the need for constant monitoring programme by the relevant regulatory agencies to ensure total compliance of the wastewater treatment facilities to the stipulated standards.
- Full Text:
Molecular studies on cholera and non-cholera causing vibrio pathogens isolated from some water resources and aquatic animals in Eastern Cape Province, South Africa
- Authors: Abioye, Oluwatayo Emmanuel
- Date: 2018
- Subjects: Vibrio cholerae -- Pathogens Vibrio cholerae
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10353/9154 , vital:34283
- Description: The global socioeconomic and disease burdens of cholera; a disease caused by Vibrio cholerae have been ongoing since it was first reported in India in 1563. Unfortunately, emerging non-cholera causing Vibrio pathogens have been contributing to the malady of Vibrio genus globally in the recent years. The history of cholera outbreaks in South Africa have been well documented but information regarding other non-cholera Vibrio pathogens, is deficient in the literature. This study evaluated the statuses of aquatic animals including crustaceans, molluscs, and fish as important reservoirs of six key pathogenic Vibrio spp. in some important water resources and fish markets in the Eastern Cape, South Africa, between December 2016 and November 2017. Physiochemical parameters that are vital for water quality assessment were determined for all the water sampling points using standard methods. The parameters include pH, electrical conductivity (EC), total dissolved solids (TDS), salinity, temperature, total dissolved solids, turbidity and biological oxygen demand (BOD5). Vibrio spp. densities in water and aquatic animal samples were determined using membrane filtration and Most Probable NumberPolymerase chain reaction (MPN-PCR) methods respectively. Presumptive Vibrio spp. were recovered from the samples by direct plating of water samples and aliquots of aquatic animal homogenates on thiosulfate-citrate-bile salts-sucrose (TCBS) agar. Where necessary, samples were enriched using alkaline peptone water (APW) for optimum isolation of the targeted Vibrio spp. Presumptive isolates were identified and delineated into the six targeted pathogenic Vibrio spp. using PCR method. The antibiogram fingerprint of selected Vibrio spp. against a panel 18 panels of antibacterial agents were also carried out. Multiple antibiotic resistance phenotypes (MARPs) per set of isolates from water sampling sites and aquatic animal types were determined. Finally, multiple antibiotic resistance indices (MARI) for isolates recovered from the samples were determined. All experiments were done in replicate and data compared using one-way analysis of variance and one-tail t-test as appropriate. Correlation and regression analysis were also carried out as appropriate. Uncontrolled agricultural, industrial and domestic wastewater influxes, as well as run-off intrusions into the water resources investigated, were observed throughout the sampling season. The physicochemical qualities of the water resources varied with seasons. While pH observed for all the water sampling sites complied with South Africa and international standards, most sampling sites did not comply with set standards for one or more of the remaining physicochemical parameters. The parameters that frequently exhibited significant correlation and regression with Vibrio spp. densities were TDS, TSS, turbidity and temperature. The densities of Vibrio spp. in the samples studied varied with season and the highest density per sample types were observed in the summer. The prevalence of Vibrio spp. in freshwater, brackish water and aquatic animal samples were 79 percent, 78 percent and 86 percent respectively. The respective prevalence of V. cholerae, V. mimicus, V. fluvialis, V.alginolyticus, V. vulnificus and V. parahaemolyticus in freshwater samples (18 percent, 3 percent, ~1 percent, ~1 percent, <1 percent, < percent), brackish water(12 percent, 3 percent, 28 percent,~1 percent, ~1 percent, 9 percent) and aquatic animals (9 percent, 1 percent, 17 percent, 1 percent, 1 percent, 17 percent) were observed. All isolates from all the sample types showed very high antibiotic resistance against ampicillin and polymyxin B. Furthermore, MARPs were observed among isolates from the three sample types. Multiple antibiotic resistance indices (MARI) above 0.2 was observed at one site on Kowie River, three on Kubusi River and one on Buffalo River. The findings of this study suggest that most South Africa water-bodies especially freshwater resources are not well protected. This need to be addressed in a country like South Africa where availability of the freshwater resource for human activities is a critical issue. Base on the prevalence of pathogenic Vibrio spp. especially MARPs and sites that exhibited above 0.2 threshold for MARI, environmental base Vibrio species monitoring programs are recommended.
- Full Text:
- Authors: Abioye, Oluwatayo Emmanuel
- Date: 2018
- Subjects: Vibrio cholerae -- Pathogens Vibrio cholerae
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10353/9154 , vital:34283
- Description: The global socioeconomic and disease burdens of cholera; a disease caused by Vibrio cholerae have been ongoing since it was first reported in India in 1563. Unfortunately, emerging non-cholera causing Vibrio pathogens have been contributing to the malady of Vibrio genus globally in the recent years. The history of cholera outbreaks in South Africa have been well documented but information regarding other non-cholera Vibrio pathogens, is deficient in the literature. This study evaluated the statuses of aquatic animals including crustaceans, molluscs, and fish as important reservoirs of six key pathogenic Vibrio spp. in some important water resources and fish markets in the Eastern Cape, South Africa, between December 2016 and November 2017. Physiochemical parameters that are vital for water quality assessment were determined for all the water sampling points using standard methods. The parameters include pH, electrical conductivity (EC), total dissolved solids (TDS), salinity, temperature, total dissolved solids, turbidity and biological oxygen demand (BOD5). Vibrio spp. densities in water and aquatic animal samples were determined using membrane filtration and Most Probable NumberPolymerase chain reaction (MPN-PCR) methods respectively. Presumptive Vibrio spp. were recovered from the samples by direct plating of water samples and aliquots of aquatic animal homogenates on thiosulfate-citrate-bile salts-sucrose (TCBS) agar. Where necessary, samples were enriched using alkaline peptone water (APW) for optimum isolation of the targeted Vibrio spp. Presumptive isolates were identified and delineated into the six targeted pathogenic Vibrio spp. using PCR method. The antibiogram fingerprint of selected Vibrio spp. against a panel 18 panels of antibacterial agents were also carried out. Multiple antibiotic resistance phenotypes (MARPs) per set of isolates from water sampling sites and aquatic animal types were determined. Finally, multiple antibiotic resistance indices (MARI) for isolates recovered from the samples were determined. All experiments were done in replicate and data compared using one-way analysis of variance and one-tail t-test as appropriate. Correlation and regression analysis were also carried out as appropriate. Uncontrolled agricultural, industrial and domestic wastewater influxes, as well as run-off intrusions into the water resources investigated, were observed throughout the sampling season. The physicochemical qualities of the water resources varied with seasons. While pH observed for all the water sampling sites complied with South Africa and international standards, most sampling sites did not comply with set standards for one or more of the remaining physicochemical parameters. The parameters that frequently exhibited significant correlation and regression with Vibrio spp. densities were TDS, TSS, turbidity and temperature. The densities of Vibrio spp. in the samples studied varied with season and the highest density per sample types were observed in the summer. The prevalence of Vibrio spp. in freshwater, brackish water and aquatic animal samples were 79 percent, 78 percent and 86 percent respectively. The respective prevalence of V. cholerae, V. mimicus, V. fluvialis, V.alginolyticus, V. vulnificus and V. parahaemolyticus in freshwater samples (18 percent, 3 percent, ~1 percent, ~1 percent, <1 percent, < percent), brackish water(12 percent, 3 percent, 28 percent,~1 percent, ~1 percent, 9 percent) and aquatic animals (9 percent, 1 percent, 17 percent, 1 percent, 1 percent, 17 percent) were observed. All isolates from all the sample types showed very high antibiotic resistance against ampicillin and polymyxin B. Furthermore, MARPs were observed among isolates from the three sample types. Multiple antibiotic resistance indices (MARI) above 0.2 was observed at one site on Kowie River, three on Kubusi River and one on Buffalo River. The findings of this study suggest that most South Africa water-bodies especially freshwater resources are not well protected. This need to be addressed in a country like South Africa where availability of the freshwater resource for human activities is a critical issue. Base on the prevalence of pathogenic Vibrio spp. especially MARPs and sites that exhibited above 0.2 threshold for MARI, environmental base Vibrio species monitoring programs are recommended.
- Full Text:
Monetary policy credibility, exchange rate pass through and inflation in South Africa
- Authors: Bom, Sandisiwe Abongile
- Date: 2018
- Subjects: Foreign exchange rates -- South Africa Monetary policy -- South Africa
- Language: English
- Type: Thesis , Masters , MCom (Economics)
- Identifier: http://hdl.handle.net/10353/13303 , vital:39632
- Description: The South African Reserve Bank (SARB) implemented inflation targeting as a monetary policy framework in 2000 with the aim of achieving low and stable price associated with economic growth. However, the period in which the framework was implemented is characterised by periods of price instability and low economic growth. This contradicts the view of different views in the literature that indicates that inflation targeting ensures low inflation rates. The purpose of the current study was to investigate the nexus between monetary policy credibility, exchange rate pass-through and inflation in South Africa. The study employed the Johansen co-integration test, Vector error correction modelling (VECM) techniques, impulse response and variance decomposition for the period from 2000 to 2017 using quarterly data. The Johansen cointegration indicated that there was a long-term relationship between the variables of interest. The VECM was estimated together with the impulse response and variance decomposition. The empirical results indicated that the variables utilised in the study are positively related. Impulse response functions also proved that in the long run, changes in consumer prices are a result of fluctuations in oil prices and the repo rate. It is evident that consumer prices (inflation) are positively related to changes in exchange rates and monetary policy credibility. Thus, the study recommends that changes in the exchange rate must be considered when implementing the monetary policy as the prices move in the same direction as changes in the NEER. Further, the lack of monetary policy credibility seems to be an issue for monetary authorities as there is supposed to be an inverse relationship among the CPI and MPCRED, which is opposed by the study. Additionally, the results also indicate that the repo rate is positively related to the consumer price and this opposes previous literature. Thus, recommending that the SARB needs to evaluate the monetary policy or the inflation targeting framework to ensure they achieve credibility
- Full Text:
- Authors: Bom, Sandisiwe Abongile
- Date: 2018
- Subjects: Foreign exchange rates -- South Africa Monetary policy -- South Africa
- Language: English
- Type: Thesis , Masters , MCom (Economics)
- Identifier: http://hdl.handle.net/10353/13303 , vital:39632
- Description: The South African Reserve Bank (SARB) implemented inflation targeting as a monetary policy framework in 2000 with the aim of achieving low and stable price associated with economic growth. However, the period in which the framework was implemented is characterised by periods of price instability and low economic growth. This contradicts the view of different views in the literature that indicates that inflation targeting ensures low inflation rates. The purpose of the current study was to investigate the nexus between monetary policy credibility, exchange rate pass-through and inflation in South Africa. The study employed the Johansen co-integration test, Vector error correction modelling (VECM) techniques, impulse response and variance decomposition for the period from 2000 to 2017 using quarterly data. The Johansen cointegration indicated that there was a long-term relationship between the variables of interest. The VECM was estimated together with the impulse response and variance decomposition. The empirical results indicated that the variables utilised in the study are positively related. Impulse response functions also proved that in the long run, changes in consumer prices are a result of fluctuations in oil prices and the repo rate. It is evident that consumer prices (inflation) are positively related to changes in exchange rates and monetary policy credibility. Thus, the study recommends that changes in the exchange rate must be considered when implementing the monetary policy as the prices move in the same direction as changes in the NEER. Further, the lack of monetary policy credibility seems to be an issue for monetary authorities as there is supposed to be an inverse relationship among the CPI and MPCRED, which is opposed by the study. Additionally, the results also indicate that the repo rate is positively related to the consumer price and this opposes previous literature. Thus, recommending that the SARB needs to evaluate the monetary policy or the inflation targeting framework to ensure they achieve credibility
- Full Text: