Exploring rural youth livelihood opportunities: a case study of a youth centre in Bizana
- Authors: Mtwa, Zikhona Asanda
- Date: 2023-10-13
- Subjects: Rural youth South Africa Bizana , Youth centers South Africa Bizana , Sustainable livelihood , NEET
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/431485 , vital:72776
- Description: Throughout South Africa, youth that are not in Education, Employment, or Training (NEET) continue to face many socio-economic challenges, including unemployment. Bizana, a small rural town in the Eastern Cape, is no exception. This study explores rural youth livelihood opportunities for youth NEET in Bizana. There is limited research done to understand the role of youth centres in providing accessing to rural youth livelihood opportunities. As a result, this study sought to understand the role of youth centres in re-integrating youth NEET into the labour market, as well as into education and training institutions. It utilizes the case study of the Bizana Love Life youth centre by looking at the programmes they offer, and whether they enhance livelihood opportunities for youth NEET. The Sustainable Livelihoods Approach (SLA) was used as a theoretical lens in understanding rural youth livelihood opportunities for youth NEET in Bizana, by identifying the challenges they face and the assets they use to enhance their livelihoods. Against this backdrop, the study adopted a qualitative research approach and an explorative case study design and was supplemented by purposive and snowball sampling methods. Data was obtained using semi-structured interviews with twelve youth NEET participants who are part of the programmes of the youth centre, and two staff members of the youth centre. Findings were analysed using open coding and thematic analysis in line with the objectives of the study. The findings of the study revealed that in attempting to gain employment, extended family support, parenthood, financial reasons, household chores, poor academic performance and lack of work experience were some of the challenges facing rural youth NEET in Bizana. The findings indicate that a combination of human and social capital can be used to support youth NEET in gaining confidence and in preparing them for the workplace. With these findings, the study indicates the need to strengthen existing youth programmes that cater for youth NEET, who are the most vulnerable in the labour market, through skills development and broadening of their social networks. As a policy recommendation, the national and provincial government must work with youth centres and other stakeholders to establish policies that cater for the development of youth NEET. , Thesis (MSocSci) -- Faculty of Humanities, Sociology, 2023
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- Date Issued: 2023-10-13
The development and op timisation of a Theiler’s murine encephalomyelitis virus antiviral assay
- Authors: Naidoo, Urisha Tirah
- Date: 2023-10-13
- Subjects: Theiler's encephalomyelitis virus , Picornaviruses , Antiviral agents , Immunofluorescence , Western immunoblotting , Drug development
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/424677 , vital:72174
- Description: Picornaviruses belong to the Picornaviridae family which are one of the largest and most diverse family of RNA viruses that cause a broad spectrum of infections in both humans and animals. These diseases range from severe infections such as poliomyelitis, meningitis, myocarditis to mild illnesses such as the common cold. Picornavirus outbreaks are a worldwide threat as they are continuously occurring. A recent outbreak of foot-and-mouth disease caused by a picornavirus occurred in South Africa, resulting in a temporary ban on the movement of cattle. Currently, the FDA has not approved any antiviral drugs against this virus, increasing the urgency for identifying effective antivirals. Picornaviruses have similar genomes and capsid organisation as such, those that are non-hazardous to humans can be used as a model system. A Theiler’s murine encephalomyelitis virus (TMEV) strain GDVII and Baby Hamster Kidney fibroblasts (BHK-21 cells) was used as a replication system to develop and optimise a medium-throughput antiviral screening assay. The TMEV GDVII replication system in BHK-21 cells was validated, and preliminary experiments were performed that were necessary for the development of the TMEV GDVII antiviral assay. This was achieved by conducting a CPE assay to visually monitor the onset and development of CPE induced by TMEV GDVII. Plaque assays accurately quantified the number of infectious virus particles required for calculating the MOI in downstream experiments. Lastly, indirect immunofluorescence and Western blot analysis detected the expression of viral proteins using previously generated antibodies against the TMEV GDVII VP1 capsid and 2C protein, thereby confirming infection in BHK-21 cells. The development of robust and reproducible assays is an essential component in antiviral drug discovery. Therefore, the confirmed replication system was then used as a foundation to develop a medium-throughput CPE-based TMEV GDVII antiviral assay whereby the parameters were optimised to produce one of high quality. Firstly, the quantitation of viral-induced CPE was examined and confirmed in a 96-well plate using resazurin as a cell viability indicator. Each parameter was tested at varying conditions, and the optimal was concluded as 2 % FBS in the assay media, a 15 000 cells/well seeding density, infecting the cells with TMEV GDVII at an MOI of 0.00625 and measuring resazurin at an endpoint of 72 hpi. Furthermore, the parameters were ii validated by calculating the Z’- factor, which consistently produced scores above 0.5, indicative of a reliable, robust, reproducible antiviral assay. Currently, there are no inhibitors against TMEV GDVII that have been reported or confirmed in cell lines, animal models or clinical trials. Therefore, once the optimal assay parameters were selected, it presented an opportunity to assess whether potential compounds, including itraconazole (ITZ) and dipyridamole (DIP), possessed antiviral activity that could firstly, be utilised as a control inhibitor when screening compounds against TMEV GDVII and secondly, contribute to research on this virus. Additionally, the previously produced anti-TMEV GDVII capsid antibody was shown to neutralise viral infection and was also included as a potential control. The sensitivity of the cells towards DMSO, a solution in which the compounds were solubilised, was first investigated. It was found that concentrations above 1 % are toxic to the cells; as such, the final DMSO concentrations were always kept below 1 % when screening compounds. Lastly, the generation of dose-response curves aided in the conclusion that the antibody was the most suitable control inhibitor as it displayed potent antiviral activity and no cytotoxicity towards the cells. In contrast, ITZ and DIP did not possess effective antiviral action and were toxic to cells at high concentrations. Finally, after all the components of the medium-throughput TMEV GDVII antiviral assay were identified, it was possible to screen 24 compounds from a coumarin and marine natural product library for cell cytotoxicity and antiviral activity. After generating dose-response curves, it was concluded that no compound effectively inhibited virus-induced CPE, and most were toxic to cells at relatively high concentrations. In conclusion, this is the first study that describes the development and optimisation of a robust medium-throughput CPE-based antiviral assay that has immense potential to screen other libraries of compounds for antiviral activity against TMEV GDVII. , Thesis (MSc) -- Faculty of Science, Biochemistry and Microbiology, 2023
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- Date Issued: 2023-10-13
Simplified menu-driven data analysis tool with macro-like automation
- Authors: Kazembe, Luntha
- Date: 2022-10-14
- Subjects: Data analysis , Macro instructions (Electronic computers) , Quantitative research Software , Python (Computer program language) , Scripting languages (Computer science)
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/362905 , vital:65373
- Description: This study seeks to improve the data analysis process for individuals and small businesses with limited resources by developing a simplified data analysis software tool that allows users to carry out data analysis effectively and efficiently. Design considerations were identified to address limitations common in such environments, these included making the tool easy-to-use, requiring only a basic understanding of the data analysis process, designing the tool in manner that minimises computing resource requirements and user interaction and implementing it using Python which is open-source, effective and efficient in processing data. We develop a prototype simplified data analysis tool as a proof-of-concept. The tool has two components, namely, core elements which provide functionality for the data anal- ysis process including data collection, transformations, analysis and visualizations, and automation and performance enhancements to improve the data analysis process. The automation enhancements consist of the record and playback macro feature while the performance enhancements include multiprocessing and multi-threading abilities. The data analysis software was developed to analyse various alpha-numeric data formats by using a variety of statistical and mathematical techniques. The record and playback macro feature enhances the data analysis process by saving users time and computing resources when analysing large volumes of data or carrying out repetitive data analysis tasks. The feature has two components namely, the record component that is used to record data analysis steps and the playback component used to execute recorded steps. The simplified data analysis tool has parallelization designed and implemented which allows users to carry out two or more analysis tasks at a time, this improves productivity as users can do other tasks while the tool is processing data using recorded steps in the background. The tool was created and subsequently tested using common analysis scenarios applied to network data, log data and stock data. Results show that decision-making requirements such as accurate information, can be satisfied using this analysis tool. Based on the functionality implemented, similar analysis functionality to that provided by Microsoft Excel is available, but in a simplified manner. Moreover, a more sophisticated macro functionality is provided for the execution of repetitive tasks using the recording feature. Overall, the study found that the simplified data analysis tool is functional, usable, scalable, efficient and can carry out multiple analysis tasks simultaneously. , Thesis (MSc) -- Faculty of Science, Computer Science, 2022
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- Date Issued: 2022-10-14
Mental Health Professionals’ Gender-Sensitivity and Responsiveness to the Genderqueer population in Substance Use Disorder Treatment: A Systematic Review
- Authors: Maseko, Moosa Lorenzo
- Date: 2021-10-29
- Subjects: Gender nonconformity , Substance abuse , Mental health personnel , Gender-nonconforming people Counseling of , Discrimination in mental health services , Joanna Briggs Institute’s systematic review method
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/190133 , vital:44966
- Description: Background: The prevalence of Substance Use Disorders (SUDs) amongst the genderqueer population is a huge concern in the public mental health system. The genderqueer population’s help-seeking barriers have been attributed to SUD treatment centre’s questionable ability to be responsive to the unique mental health needs of genderqueer individuals. Aim: The aim of this study is to conduct a systematic review on mental healthcare workers‟ responsiveness and gender-sensitivity towards the genderqueer population in SUD treatment centres. Methods: Employing the Joanna Briggs Institute’s (JBIs) systematic review method, 25 qualitative articles were included in this study. A thematic analysis was used to examine the data. Results: The analysis revealed that SUD treatment centres are experienced as discriminatory and unreceptive by the genderqueer population due to several barriers. The barriers identified were structural, financial, personal, cultural and the use of a heterosexual framework to treat SUD which led to abuse, isolation, and stigma. Mental healthcare providers lack skills in working with genderqueer individuals as well as a lack of knowledge on genderqueer related needs. Lack of gender sensitivity affects genderqueer individuals in accessing SUD treatment centres and the progress they make. This magnified the need and importance of specialised gender-responsive and gender-sensitive training in working with genderqueer individuals. Twelve interventions to address the areas of difficulty were identified. , Thesis (MA) -- Faculty of Humanities, Psychology, 2021
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- Date Issued: 2021-10-29
Psychosocial experiences of caregivers of mentally challenged children in Alice, Eastern Cape
- Authors: Mmangaliso, Azola
- Date: 2021-08
- Subjects: Caregivers , Children with disabilities--Psychology , Children with mental disabilities
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/21025 , vital:46904
- Description: There is dearth of information in South Africa about the psychosocial challenges experienced by caregivers of mentally challenged children. This study addresses psychosocial experiences of caregivers, such as, scorning, isolation and anxiety, which affect their well-being in adverse ways, and that continues to be overlooked by professionals dealing with mental health. This study sought to explore psychosocial experiences of caregivers of mentally challenged children in Alice in the Eastern Cape in South Africa. The study intended to uncover the psychosocial wellbeing of caregivers of mentally challenged children. Secondly, it set out to highlight the coping mechanisms employed by caregivers of mentally challenged children to deal with their psychosocial experiences. Lastly, the study sought to examine policies, legislation and support services in place for psychosocial care of caregivers of mentally challenged children. The researcher adopted qualitative research methods. Snowball sampling was employed to recruit 15 caregivers of mentally challenged children in Alice. The age of the caregivers ranged between the ages of 20 and 65. In addition, purposive sampling was adopted to recruit six professionals working with mental health in Alice. All the participants were black Africans. The gender comprised of thirteen females and two males. Data was collected through the use of face-to-face, semi-structured individual interviews, one interview per research participant, with the caregivers of mentally challenged children, and through the use of one focus group discussion with professionals dealing with mental health. The researcher analysed data through thematic content analysis. The study findings indicate that caregivers of mentally challenged children experience the caregiving process negatively, with a lot of burden. It also appears that they do not cope positively with such experiences despite professionally-trained personnel that are obliged by the Social Assistance Act and other government policies to ensure that caregivers receive sufficient psychosocial services. Findings also indicate that there is very little that is done by the relevant professionals in ensuring that they render sufficient professional support services to caregivers, such as needs assessment and therapeutic services. In light of the findings, the study concludes that there is a need for continuous rendering of comprehensive psychosocial support services to the caregivers of mentally challenged children. It is also recommended that social workers empower caregivers for mentally challenged children so that they can function effectively in their communities. Further, the government needs to reinforce existing policies that afford these psychosocial services, such as Integrated National Strategy on Support Services to Children with Disabilities. Meaning, the government should take an oversite role of these policies and monitor whether they get implemented or not, especially at grassroots level. , Thesis (MSoc Sci) (Social Work) -- University of Fort Hare, 2021
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- Date Issued: 2021-08
Kwelo lizwe leentsomi : iintsomi zesiXhosa
- Authors: Sigcau, Bulelwa Monica
- Date: 2021-04
- Subjects: Creative writing (Higher education) -- South Africa , Xhosa fiction -- 21st century , Diaries -- Authorship , Short stories, Xhosa -- 21st century , African fiction -- History and criticism , English fiction -- History and criticism
- Language: Xhosa
- Type: thesis , text , Masters , MA
- Identifier: http://hdl.handle.net/10962/178270 , vital:42926
- Description: Ukuphosa kuvimba wolwazi ngeentsomi zesiXhosa, le thisisi iqulathe iintsomi-mabali eziqanjwe zabaliswa kusetyenziswa ulwimi, umxholo nemixholo engenelelayo engeziganeko zemihla ngemihla, abalinganiswa, isakhiwo sentsomi nesimo sentlalo zala maxesha siphila kuwo. Ezi ntsomi zixhaswa kakhulu yimifanekiso ephilayo, ukusetyenziswa kwemigca emifutshane ezoba isimo sentlalo nevakala ingathi yimibongo ngenxa yokuqupha kwayo. Umsebenzi kaR.F. Mcimeli, kwincwadi ethi, Ingqaka kaMaqhudeni iqulethe iintsomi ezisondeleyo kwezi ziqanjwe kulo msebenzi, ngakumbi iintsomi ezizezi, uNomvula noNomvulazana, uDumisani nesigebenga esingabonwayo, uNomaciko noCikokazi, nezinye. Umsebenzi kaNongenile Masithathu Zenani (2006) oqokelelwe nguHarold Scheub kwincwadi ethi South African voices: A long time passed ube negaleloo elikhulu ukubonisa indima esele idlaliwe kwiintsomi zesiXhosa. UKholeka Sigenu (2002) kwincwandi ethi Ezakowethu naye ube nefuthe kwindlela ezi ntsomi eziqanjwe ngayo nokuqinisekisa ukuba ngenene zongeza ulwazi olutsha. Ezi ntsomi-mabali zibhentsise okulungileyo nokuhenxayo ngaphandle kokushumayela nokuveza abalinganiswa njengeengilosi. Ndiluthande kakhulu nohlobo lukaAmos Tutuola lokubhala, ndizame ukusondeza uhlobo lwam lokubhala kolu hlobo lukaAmos Tutuola. Ndilandele ifuthe lohlobo lokubhala olubizwa ngeflash fiction. , Thesis (MA) -- Faculty of Humanities, School of Languages and Literatures, 2021
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- Date Issued: 2021-04
Synthesis, characterisation and evaluation of ferrocene-containing Novobiocin analogues for anticancer and antiplasmodial activity through inhibition of Hsp90
- Authors: Mbaba, Mziyanda
- Date: 2017
- Subjects: Antibiotics Synthesis , Ferrocene , Heat shock proteins , Antimalarials , Cancer Chemotherapy
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/65111 , vital:28690
- Description: Novobiocin (Nb) is a coumarin type antibiotic isolated from the bacterium species of Streptomyces and possesses modest anticancer and antimalarial activities. Nb and analogues have been extensively explored as potential anticancer agents through inhibition of the C- terminal domain of heat shock protein 90 (Hsp90), which plays a pivotal role in the proteinfolding machinery of cells. There has been little effort in the exploration of Nb and derivatives for antimalarial activity. Incorporation of organometallic units, such as ferrocene (Fc), into bioactive chemical scaffolds remains an attractive approach for developing new therapeutic agents for treatment of several ailments. The current study sought to investigate the anticancer and antiplasmodial effects of incorporating ferrocene (Fc) into Nb scaffold presumably through inhibition of Hsp90. The ferrocenyl Nb analogues containing simplified structural motifs such as phenyl, benzyl, and piperidine were synthesized in six to nine steps employing conventional synthetic organic protocols adapted from literature, and the compounds were accessed in reasonable yields. For comparison purposes, a selection of organic Nb analogues were also included in the study. The target compounds were characterized by spectroscopic techniques including 1-dimensional nuclear magnetic resonance (1D NMR) and high-resolution mass spectroscopy. The synthesized compounds were evaluated in vitro for potential anticancer and antiplasmodial activities using the breast cancer cell line (HCC38) and chloroquine-sensitive strain (3D7) of the malaria parasite, Plasmodium falciparum. The presence of the Fc unit was found to enhance both anticancer and antiplasmodial activities of the resultant ferrocenyl Nb compounds with IC50 values in the low to mid micromolar range. Hsp90 inhibitory studies of the ferrocenyl Nb analogues possessing superior activities (2.13a and 2.20c) were also conducted using different yeast strains expressing both human and malarial Hsp90 isoforms: hHsp90a/p and PfHsp90, respectively. The results of Hsp90 inhibitory studies suggested no direct correlation between the observed activities of the analogues and Hsp90 inhibition. However, since the conditions of the assay were not optimised due to time constrains of the project, these observed data remained to be confirmed. , Thesis (MSc) -- Faculty of Science, Chemistry, 2017
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- Date Issued: 2017