Understanding the sexual risk behaviours of people living with different mental illnesses and their vulnerability to HIV infection
- Authors: Ndlovu, Deogracious
- Date: 2013
- Subjects: Health -- Social aspects , HIV infections
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/25037 , vital:63899
- Description: This study was primarily carried out in order to assess the sexual risk behaviours of people living with mental illnesses as well as their vulnerability to HIV infection. It sets out the basic realities of the interaction between mental illness and HIV/AIDS and its impact on individuals and communities. This study explores the implications for health policy and practice, and suggests how to provide better support and assistance in the empowerment of people living with mental illness as far as HIV and AIDS is concerned, including community support. It was also the aim of this study to look into the factors that expose this group of people to HIV infection as well as to understand the link between HIV/AIDS and mental illness. It also tested the inclusion of people with mental disabilities in HIV and AIDS prevention and education programmes. The research was conducted on the basis of a qualitative research design. A semi-structured interview schedule was used in broad based interviews to gather information from caregivers or guardians of people with mental disabilities as well as the professionals in the mental health sector. The results of the research revealed that people who are living with mental illnesses are a vulnerable group as regards HIV infection. It also conveys that this group of people presents with sexual risk behaviours, which increase their vulnerability to HIV and AIDS. In addition, there are many barriers against the inclusion of this group of people in HIV and AIDS prevention and education programmes and, as a result, they are often excluded from participating in such programmes. Importantly, the findings of this study acknowledge that there is a two-fold relationship between mental illness and HIV/AIDS. It indicates that mental illnesses are still regarded as mysterious in the community and there is much stigma and discrimination towards the mentally ill, increasing their vulnerability to HIV infection. , Thesis (MSoc) -- Faculty of Social Sciences and Humanities, 2013
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- Date Issued: 2013
Optimisation of expression of a rice (Oryza sativa L.cv Nipponbare) plant natriuretic peptide (OsPNP-B) and its functional characterisation
- Authors: Affun, Ogheneochuko Janet
- Date: 2012
- Subjects: Rice , Plant hormones , Water-electrolyte imbalances
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/24354 , vital:62644
- Description: Maintenance of water and solute homeostasis is a key requirement for living systems, and in vertebrates, homeostasis is in part achieved by natriuretic peptides (NP), a family of peptide hormones. A related family of peptide hormones have also been found in the plant kingdom. Plant natriuretic peptides (PNP) are a novel class of plant proteins with two closely related homologous genes (AtPNP-A and AtPNP-B) identified in the model plant Arabidopsis thaliana. AtPNP-A has been extensively studied and evidence obtained points to a role in plant water homeostasis. No research has been conducted on the function of PNP-B proteins. In this study, we focus on the bioinformatic analysis of the PNP-B gene in various plants, as well investigating whether PNP-B plays a role in water homeostasis in rice plants exposed to drought stress. Basic local alignment search tool (BLAST) queries of the ―The Gene Index‖, EST and available plant genome databases revealed the presence of the PNP-B mRNA in rice, oil seed rape, oak, leafy spurge, poplar, sugarcane, pepper, cotton, apple and maize. All the identified genome sequences contained a predicted intron/insert, which was not present in the related mRNA sequences. However, RT-PCR analysis revealed the presence of the 101 bp insert in the 976 bp amplified rice PNP-B (OsPNP-B) cDNA and therefore expression was optimized for the protein encoded by only the exon2 sequence as this contained the predicted active site region of PNP-B. OsPNP-B was shown to be translated to a protein of 14 kDa with a sequence similarity to AtPNP-B (54percent), AtPNP-A (37percent) and CjBAp12 (55percent), suggesting two possible functions for PNP-B viz water homeostasis and/or pathogenesis defence. To determine whether PNP-B is involved in water homeostasis, total protein extracted from 4 weeks old (4 leaves stage) rice plants subjected to drought treatment for a period of 24, 48, 72, 120, 168 and 240hrs were resolved by 17percent SDS-PAGE and analysed by western blot analysis. The PNP-B protein was found to be down-regulated during drought stress, implying that PNP-B may play a role in water homeostasis through the release of water from cells rather than the up-take of water as seen At-PNP-A. PNP-B could therefore also be involved in plant defence mechanisms to pathogens where plants induce desiccation of infected leaves, thereby ridding the plant of the relevant pathogen. , Thesis (MSc) -- Faculty of Science and Agriculture, 2012
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- Date Issued: 2012
Impact of performance evaluation on service rendering by the Department of Education in the Lady Frere District at selected schools (2010-2011)
- Authors: Ngodwane, Anele Anthony
- Date: 2012-01
- Subjects: Performance standards , Education and state , School management and organization
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/26437 , vital:65312
- Description: The aim of this study is to evaluate the impact of performance evaluation on the service rendering by the department of education. There have been studies made around performance management system but to my knowledge it was the first of this nature in Lady Frere District. Performance Management System has not been a success ever since its implementation. A lot had happened in our country from apartheid era to the present democratic dispensation. Among the achievements South Africa masters is the ability to formulate or the formulation of policies but its weakness is on the implementation. Performance management system is among the policies that were developed but its implementation has never been a success. Its main aim is to enhance service delivery and attempt to make government effective and efficient. This research considers if performance management system and performance evaluation might address the question of service delivery in the Department of Education in Lady Frere District. Findings on this research spell it out that effective implementation performance management system will lead to an enhanced service delivery. , Thesis (MPA) -- Faculty of Management and Commerce , 2012
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- Date Issued: 2012-01