Exploring the involvement of the SARS-CoV-2 Replicase in the synthesis of polyamines and heat shock proteins
- Authors: Mthembu,Yamkela
- Date: 2023-07
- Subjects: Post COVID-19 condition (Disease) , COVID-19 (Disease) , Polyamines in the body
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/${Handle} , vital:74275
- Description: A disease named COVID-19 almost destroyed the human population. It is caused by the SARS CoV 2 virus which emerged in 2019 and rapidly spread worldwide forcing all countries to shut down. This virus uses non-structural proteins to complete its invasion and development inside the host. The non-structural protein 2 NSP2 defined as the RNA binding protein involved in coronavirus genome replication and decreases the human immune response is key for this virus’s development and invasion. It is believed that the NSP2 associate themselves with polyamines and heat shock proteins inside the host cell, to proceed with the viral development and this study aimed to investigate how SARS CoV 2 virus key non-structural proteins NSP2 utilizes polyamines and heat shock proteins using the molecular docking approach and molecular dynamics MD. In this present study a docking approach was used. The dockings were done on ClusPro, and analysis was done on Discovery Studio, chimera, and PyMOL. One of the enzymes that are involved in the regulation of polyamine biosynthesis, adenosylmethionine decarboxylase AdoMetDC was used in investigating the polyamine binding to the viral NSP2, and major heat shock proteins HSPs HSP40, HSP70, and HSP90 were used in the investigation of the binding of HSPs to viral non-structural proteins NSP2. The results obtained show that 32 interactions were formed when docking heat shock protein 40 HSP40 with the SARS CoV 2 NSP2, but the area that has the highest interactions was from amino acid Ala554 to His557. Asp553 plays a very crucial role in this interaction forming six interactions. Heat shock protein 70 produced about 28 interactions when docked with the SARS CoV 2 NSP2. But the section with the most bonds was from position 550 to 560 of the ligand. Human heat shock protein 90 HSP90 formed 19 interactions when docked with the SARS CoV 2 viral NSP2. MD revealed Arg458, Asn508, Met297, Arg301 and Tr417 active residues. And pharmacophore modeling indicated zinc inhibitors as crucial for inhibiting this virus. Enough interactions were produced with ideal bonds lengths, and it can be said that the functioning of the SARS CoV2 viral non-structural proteins functioning majorly depends on the presence of the heat shock proteins at hand for proper formation and depends on polyamines for complete development and correct functioning. , Thesis (MSc) -- Faculty of Science and Agriculture, 2023
- Full Text:
- Date Issued: 2023-07
- Authors: Mthembu,Yamkela
- Date: 2023-07
- Subjects: Post COVID-19 condition (Disease) , COVID-19 (Disease) , Polyamines in the body
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/${Handle} , vital:74275
- Description: A disease named COVID-19 almost destroyed the human population. It is caused by the SARS CoV 2 virus which emerged in 2019 and rapidly spread worldwide forcing all countries to shut down. This virus uses non-structural proteins to complete its invasion and development inside the host. The non-structural protein 2 NSP2 defined as the RNA binding protein involved in coronavirus genome replication and decreases the human immune response is key for this virus’s development and invasion. It is believed that the NSP2 associate themselves with polyamines and heat shock proteins inside the host cell, to proceed with the viral development and this study aimed to investigate how SARS CoV 2 virus key non-structural proteins NSP2 utilizes polyamines and heat shock proteins using the molecular docking approach and molecular dynamics MD. In this present study a docking approach was used. The dockings were done on ClusPro, and analysis was done on Discovery Studio, chimera, and PyMOL. One of the enzymes that are involved in the regulation of polyamine biosynthesis, adenosylmethionine decarboxylase AdoMetDC was used in investigating the polyamine binding to the viral NSP2, and major heat shock proteins HSPs HSP40, HSP70, and HSP90 were used in the investigation of the binding of HSPs to viral non-structural proteins NSP2. The results obtained show that 32 interactions were formed when docking heat shock protein 40 HSP40 with the SARS CoV 2 NSP2, but the area that has the highest interactions was from amino acid Ala554 to His557. Asp553 plays a very crucial role in this interaction forming six interactions. Heat shock protein 70 produced about 28 interactions when docked with the SARS CoV 2 NSP2. But the section with the most bonds was from position 550 to 560 of the ligand. Human heat shock protein 90 HSP90 formed 19 interactions when docked with the SARS CoV 2 viral NSP2. MD revealed Arg458, Asn508, Met297, Arg301 and Tr417 active residues. And pharmacophore modeling indicated zinc inhibitors as crucial for inhibiting this virus. Enough interactions were produced with ideal bonds lengths, and it can be said that the functioning of the SARS CoV2 viral non-structural proteins functioning majorly depends on the presence of the heat shock proteins at hand for proper formation and depends on polyamines for complete development and correct functioning. , Thesis (MSc) -- Faculty of Science and Agriculture, 2023
- Full Text:
- Date Issued: 2023-07
An evaluation of the impact of social media on youth political participation in South Africa's 2014 general election: the case of Fort Hare students
- Authors: Mhlomi, Yolisa
- Date: 2014-12
- Subjects: Political participation -- South Africa , Elections -- South Africa , Universities and colleges -- South Africa Maps
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/26255 , vital:65225
- Description: The aim of this paper is to evaluate the impact that social media had on influencing youths to engage in political and civic participation in the campaigning for and national elections of 2014, South Africa. Different views may impact on the implementation and use of social networking sites for politics. Youths consist of a larger population in the country but young people are also considered to be apathetic in political activities. However, it is essential to understand the perceptions of youth as this strategy was implemented especially to reach out to them, as they are the beneficiaries of the democratic system as well as future leaders of the country. Using the quantitative method, 200 questionnaires were distributed to the respondents in the University of Fort Hare and analysed by the researcher. The results of the study show that, youth are not entirely apathetic to politics and the use of social media for campaigning was seen as having a positive impact on voters because it managed to shift some youth’s perspectives about voting and how important it is for them to participate in the electoral process. , Thesis (MSoc) -- Faculty of Social Sciences and Humanities, 2014
- Full Text:
- Date Issued: 2014-12
- Authors: Mhlomi, Yolisa
- Date: 2014-12
- Subjects: Political participation -- South Africa , Elections -- South Africa , Universities and colleges -- South Africa Maps
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/26255 , vital:65225
- Description: The aim of this paper is to evaluate the impact that social media had on influencing youths to engage in political and civic participation in the campaigning for and national elections of 2014, South Africa. Different views may impact on the implementation and use of social networking sites for politics. Youths consist of a larger population in the country but young people are also considered to be apathetic in political activities. However, it is essential to understand the perceptions of youth as this strategy was implemented especially to reach out to them, as they are the beneficiaries of the democratic system as well as future leaders of the country. Using the quantitative method, 200 questionnaires were distributed to the respondents in the University of Fort Hare and analysed by the researcher. The results of the study show that, youth are not entirely apathetic to politics and the use of social media for campaigning was seen as having a positive impact on voters because it managed to shift some youth’s perspectives about voting and how important it is for them to participate in the electoral process. , Thesis (MSoc) -- Faculty of Social Sciences and Humanities, 2014
- Full Text:
- Date Issued: 2014-12
Critical assessment of leadership skills in developmental local government: a case study of Buffalo City Municipality in the Eastern Cape
- Mnyatheli, Mbeko Bandla Sizasomzi
- Authors: Mnyatheli, Mbeko Bandla Sizasomzi
- Date: 2011-01
- Subjects: Leadership , Municipal government , Buffalo City (South Africa)
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/26175 , vital:64950
- Description: This study seeks to examine the nature and role of leadership, in pursuit of a suitable leadership for a Developmental Local Government environment. The aim of this study is to describe the nature of leadership, particularly in local government, and in public organization in general. The study begins by teasing out the various concepts and terminology attendant with leadership in this environment. The study further reviews relevant literature as basis for understanding and goes on to interrogate the general theories on leadership with a view to choose the best practice in leadership of a developmental local government. Central to its quest for answers, is the drive from the constitutional mandate, a developmental local government imposed to municipality by the constitution of the Republic of South Africa, (1996) arising out of the need to transform the South African local government from an erstwhile policy of racial segregation to a new democratic political dispensation fostering equity and access to services and resources for all citizens. The challenge for the new leadership therefore is how to deal, speedily with the huge disparities and socio-economic backlogs? What kind of leadership and what level of knowledge, skills and competencies are required and how soon can public goods and services be delivered to the satisfaction of the communities it serves? ‘Analysis of variance’ was used to analyse and interpret data collected. Literature on the difference between transactional and transformational leadership formed the basis of the findings. Finally, findings and conclusions were drawn. On the bases of these, recommendations for Buffalo City Municipality were presented. , Thesis (MPA) -- Faculty of Management and Commerce, 2011
- Full Text:
- Date Issued: 2011-01
- Authors: Mnyatheli, Mbeko Bandla Sizasomzi
- Date: 2011-01
- Subjects: Leadership , Municipal government , Buffalo City (South Africa)
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/26175 , vital:64950
- Description: This study seeks to examine the nature and role of leadership, in pursuit of a suitable leadership for a Developmental Local Government environment. The aim of this study is to describe the nature of leadership, particularly in local government, and in public organization in general. The study begins by teasing out the various concepts and terminology attendant with leadership in this environment. The study further reviews relevant literature as basis for understanding and goes on to interrogate the general theories on leadership with a view to choose the best practice in leadership of a developmental local government. Central to its quest for answers, is the drive from the constitutional mandate, a developmental local government imposed to municipality by the constitution of the Republic of South Africa, (1996) arising out of the need to transform the South African local government from an erstwhile policy of racial segregation to a new democratic political dispensation fostering equity and access to services and resources for all citizens. The challenge for the new leadership therefore is how to deal, speedily with the huge disparities and socio-economic backlogs? What kind of leadership and what level of knowledge, skills and competencies are required and how soon can public goods and services be delivered to the satisfaction of the communities it serves? ‘Analysis of variance’ was used to analyse and interpret data collected. Literature on the difference between transactional and transformational leadership formed the basis of the findings. Finally, findings and conclusions were drawn. On the bases of these, recommendations for Buffalo City Municipality were presented. , Thesis (MPA) -- Faculty of Management and Commerce, 2011
- Full Text:
- Date Issued: 2011-01
- «
- ‹
- 1
- ›
- »