Beef quality, oxidative stability and fatty acid composition of Nguni heifers fed diets with incremental levels of Opuntia ficus-indica (cactus) cladodes
- Authors: Mkhosi, Silungile
- Date: 2020
- Subjects: Meat -- Quality
- Language: English
- Type: Thesis , Masters , MSc (Animal Science )
- Identifier: http://hdl.handle.net/10353/18732 , vital:42728
- Description: The objective of the study was to determine meat quality, fatty acid (FA) composition and shelf-life stability of longissimus muscle of Nguni heifers fed incremental levels of Opuntia ficus-indica (cactus) cladodes. A total of 32 heifers aged 36 months were allocated randomly to four dietary treatments: 10% cactus, 20% cactus, commercial and control diets for 90 days. The longissimus thoracis et lumborum (LTL) samples were taken from each carcass for the determination of meat quality, retail shelf-life stability and FA composition. Diet had no effect (P > 0.05) on pH and drip display, however, there was an increase in drip display with ageing time (P ≤ 0.05). Meat colour attributes (L* a* b* and C*) were influenced by diet, with 20% cactus diet having lower L* values compared to other diets (P ≤ 0.05). During a 14-day retail display, a diet day interaction was observed for hue angle with the cactus diets having the lowest values at day 14 of display compared to the other diets (P ≤ 0.05). Differences in meat from heifers fed different diets were observed with 20% cactus accounting for significantly (P ≤ 0.05) higher levels of deoxymyoglobin and the lowest levels of oxymyoglobin. Thawing loss and Warner Bratzler shear force decreased (P ≤ 0.05) with ageing period. Meat from heifers fed cactus diets had lower (P ≤ 0.05) proportions of oleic acid (C18:1n9c) and total MUFA, higher (P ≤ 0.05) proportions of total SFA, C15:1, C17:1, linoleic acid (C18:2n6c) and total PUFA. The control diet produced meat with the highest thiobarbituric acid reactive substances (TBARS) values compared to other diets (P ≤ 0.05) and with ageing TBARS values increased (P ≤ 0.05). Diet showed an effect (P ≤ 0.05) on sensory attributes such as overall tenderness and mouth feel with cactus diets accounting for lower scores. However, juiciness, overall tenderness, grassy and animal like attributes increased with ageing (P ≤ 0.05). Inclusion of cactus in the diet did not produce any adverse effects on meat quality, fatty acid composition, lipid oxidation and sensory quality. Therefore, it can be concluded that inclusion of cactus in Nguni heifer finisher diets up to 20% can improve meat quality.
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An Assessment of Teaching and Learning Strategy in Eastern Selected School, Butterworth District
- Authors: Mantanga- Mgoduka, Ntombomzi Beauty
- Date: 2019
- Subjects: School improvement programs -- South Africa -- Eastern Cape High school teaching -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MPA
- Identifier: http://hdl.handle.net/10353/12447 , vital:39264
- Description: This is a study that was conducted at Nomaheya Senior Secondary School in the Butterworth in East of the Eastern Cape Province. The main purpose of this study was to assesses teaching and learning strategies in improving Grade 12 results. Teaching and Learning Strategies are defined as activities and methods used to move the learner towards achievement of the learning outcome. Crawford, Alan; 2005:16 views teaching and learning strategy, as an effective instruction, as an interactive process - students learn best when they are engaged, not only with their teacher, but also with one another. The researcher will provide the background and motivation of the study. The problem statement, research questions, research objectives, significance of the study, delimitations of the study, literature review, research methodology, and the conclusion of the study. Poor performance that is displayed by Grade 12 learners in the Eastern Cape has been a trend for the past three years. Well known contributory factor for poor performance is poverty, unemployment, high levels of illiteracy, low levels of management and leadership in schools, constant shortage of teachers with specific reference to critical subjects, learner teacher support material (LTSM) that is not delivered on time school safety, infrastructure, discipline amongst learners, absenteeism of learners, SMT with no well-defined roles, Annual Teaching plans, circulars are available but are not implemented. In other words, lack of support by the stakeholders, management and leadership in schools. This was observed by Solo, 1997: 37 who argues that, the atmosphere at home helps to foster or break school functionality, effects of parental interest in learner academic achievement. The background of the study, the problem statement, objectives, questions and also the objectives of research were fully discussed in chapter one. In chapter two, a research on recent publications in the subject was done. Keywords were: outcomebased education, educator, change, assessment and evaluation. The literature study was done to attain objectives in chapter one and also to provide the theoretical background to evaluate the findings in chapter four. vii Chapter three dealt presents the research methodology, research design, area of study, population, sampling method, purposive sampling method, research sample, data collection procedures, data collection instruments, interviews, observations, ethical considerations.
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An assessment of teaching and learning strategy in improving grade 12 results in Eastern Cape selected school Butterworth District
- Authors: Mantanga-Mgoduka, N B
- Date: 2019
- Subjects: Educational tests and measurements Effective teaching
- Language: English
- Type: Thesis , Masters , MPA
- Identifier: http://hdl.handle.net/10353/18554 , vital:42608
- Description: This is a study that was conducted at Nomaheya Senior Secondary School in the Butterworth in East of the Eastern Cape Province. The main purpose of this study was to assesses teaching and learning strategies in improving Grade 12 results. Teaching and Learning Strategies are defined as activities and methods used to move the learner towards achievement of the learning outcome. Crawford, Alan; 2005:16 views teaching and learning strategy, as an effective instruction, as an interactive process - students learn best when they are engaged, not only with their teacher, but also with one another. The researcher will provide the background and motivation of the study. The problem statement, research questions, research objectives, significance of the study, delimitations of the study, literature review, research methodology, and the conclusion of the study. Poor performance that is displayed by Grade 12 learners in the Eastern Cape has been a trend for the past three years. Well known contributory factor for poor performance is poverty, unemployment, high levels of illiteracy, low levels of management and leadership in schools, constant shortage of teachers with specific reference to critical subjects, learner teacher support material (LTSM) that is not delivered on time school safety, infrastructure, discipline amongst learners, absenteeism of learners, SMT with no well-defined roles, Annual Teaching plans, circulars are available but are not implemented. In other words, lack of support by the stakeholders, management and leadership in schools. This was observed by Solo, 1997: 37 who argues that, the atmosphere at home helps to foster or break school functionality, effects of parental interest in learner academic achievement. The background of the study, the problem statement, objectives, questions and also the objectives of research were fully discussed in chapter one. In chapter two, a research on recent publications in the subject was done. Keywords were: outcome-based education, educator, change, assessment and evaluation. The literature study was done to attain objectives in chapter one and also to provide the theoretical background to evaluate the findings in chapter four. Chapter three dealt presents the research methodology, research design, area of study, population, sampling method, purposive sampling method, research sample, data collection procedures, data collection instruments, interviews, observations, ethical considerations.
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An evaluation of the harmonised social cash transfer programme on livelihoods in Zimbabwe: the case of Mangwe District
- Authors: Poseni ,Pedzisai
- Date: 2019
- Subjects: Economic development -- Social aspects Social integration Social systems
- Language: English
- Type: Thesis , Masters , MCom (Development Studies)
- Identifier: http://hdl.handle.net/10353/13943 , vital:39730
- Description: Poverty and limited livelihood options remains a huge challenge in Africa, particularly in Zimbabwe. The study investigated the effectiveness of the Harmonized Social Cash Transfer programme in improving household economy and strengthening livelihood options in Zimbabwe with a particular focus on Mangwe District. In order to come up with a comprehensive investigation, the researcher grounded the study in sustainable livelihood approach and the capabaility approach. Due to persistent poverty and people adopting precarious coping strategies in Zimbawe, the government and NGOs collectively came up with a comprehensive cash based poverty alleviation strategy to alleviate poverty and build on peoples livelihood options which is the cash transfer programme. The study utilized a qualitative methodology, based on its ability to provide a contextualized description of people’s perspectives, feelings and opinions in terms of how a programme affected them. The evaluation sought to find out whether the cash transfer programme achieved its objective of strengthening household economy. The study established that the Harmonized Social Cash Transfer programme made some positive inroad towards strengthening household economy, however, lack of stakeholders coordination, collapsed economy, pollitically motivated moves from the ruling party caused the programme to fail. As such, the poor Mangwe people are still continuing engaging in precarious coping strategies, such as, selling assets, eating less than one meal per day and venturing into prostitution. The study therefore, calls for the government to review the household size vs cash scale and regularly review the amount of money received to be in line with current prices of commodities
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Effects of herbicide application and phosphorous fertilization on forage production and plant density of abandoned lands planted with pasture legumes
- Authors: Mashece , Wandile
- Date: 2019
- Subjects: Forage plants Grazing
- Language: English
- Type: Thesis , Masters , MSc (Pasture Science)
- Identifier: http://hdl.handle.net/10353/11950 , vital:39121
- Description: The study was conducted in two communal abandoned grazing lands, namely; Sinqumeni and Kubedlana (referred hereafter as sites) located in the Eastern Cape Province (ECP) where legume pastures have been successfully established for the last ten years. The objectives of the study were to investigate the effects of five different herbicide treatments and four different phosphorus fertilizer application rates on forage production and plant density of abandoned lands. At each site, an area previously planted with legume species and well established with an even stand of legumes were selected. For both experimental sites, experimental design consisted of plot size of 50 m × 50 m for herbicide plot and 25 m ×25 m for phosphorus plot with 5 m×10 m of sub-plot planted with an even stand of pasture legumes arranged in a randomised complete block design (RCBD) in two blocks. The legumes that were planted in these sites were: Lotus corniculatus, White clover, Arrow leaf clover, Yellow serradella, Pitman serradella, Biserrula and Lespedeza cuneata. Prior to planting, the legume seeds were mixed with the appropriate inoculant by hand. Planting was done by over sowing legumes into the natural pastures (grasses) using an Aitchison Mini seeder. In each small plot, data was collected on forage biomass and plant density (species count). Data was analysed using the SAS programme and the significance of differences between means were tested with Fisher’s test for the LSD. On herbicide study, results showed that there were no significant (p > 0.05) difference in forage dry matter (DM) among the herbicide treatments in both years at Kubedlana. Results further showed that all five herbicides did not significantly (p > 0.05) increase the DM yield over control treatment. At Sinqumeni, results displayed no significant (p > 0.05) difference in forage DM among treatments during year 1, whereas in year 2, but all herbicide treatments except Haloxyfop-R methyl & Bendioxide (HBE) yielded greater (p < 0.05) DM yield than the control treatment. Comparing years, forage DM yield only under Bendioxide (BEN) and Paraquat (PAR) treatments had greater DM yield in year 2 than years 1. In Kubedlana, Bendioxide (BEN) and Haloxyfop-R methyl (HAL) treatments showed an IV increase in total density (TD), whereas Glyphosate (GLY) produced the lowest total density (TD) and Paraquat (PAR) and Haloxyfop-R methyl (HAL) resulted in an increased in density of total legumes (TL) relative to control treatment. There was a decline (p < 0.05) in the densities of total plants and forbs from year 1 to year 2. In Sinqumeni, herbicide applications resulted in higher density of total legumes than the control treatment, but results varied among herbicide treatments, greatest density being obtained at Haloxyfop-R methyl & Bendioxide (HBE) and Haloxyfop-R methyl (HAL) treatments. On phosphorus study, result showed a significant (p < 0.05) differences in dry matter (DM) yield among Phosphorus (P) treatments during year 2 of the study at Kubedlana site. All P treatments showed significantly greater dry matter (DM) yield in forage harvested in year 2 compared to year 1. At Sinqumeni, results showed Phosphorus (P) application did not have significance (p > 0.05) effect on forage dry matter (DM) yield on both years. In Kubedlana, there were indications of increased densities of total grasses, legumes and total plants in response to some levels of phosphorus (P) treatments. Treatment Phosphorus at 20 kg ha-1 (P20) yielded the highest total density (TD) and total legumes (TL) numbers and P10 had the lowest total density (TD). Both treatment and year interacted significantly (p < 0.05) to influence the mean density of some plant variables. At Sinqumeni, there were no significant differences due to phosphorus (P) treatments in the densities of total plants, grasses and forbs. Both years interacted significantly (p < 0.05) with treatments to influence the plant density mean, with Lespedeza cuneate (LESP) producing the highest plant density during year 1 and year 2, whereas BSERRL produced the lowest plant density during year 1 and year 2 periods. Therefore, findings of this study suggest that application of both Bendioxide (BEN) & Haloxyfop-R methyl & Bendioxide (HBE) treatments result in a decrease in dry matter (DM) yield, whereas Glyphosate (GLY) herbicide resulted in a decrease in both total density (TD) and total legumes (TL) and Haloxyfop-R methyl (HAL) herbicide application resulted in an increase of total density (TD) and total legumes (TL) V relative to control plot. On phosphorus study, results suggest that at Phosphorus at 40 kg ha-1 (P40) fertilization dry matter (DM) yield will be increased and at both Phosphorus at 20 kg ha1 (P20) and Phosphorus at 40 kg ha-1 (P40) high total density (TD) will be recorded where’s Phosphorus at 20 kg ha-1 (P20) fertilization result in high total legumes (TL).
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Preparation, characterization and in vitro analysis of polyamidoamine drug conjugates containing ferrocene and platinum analogues
- Authors: Mugogodi, Ansley
- Date: 2018
- Subjects: Ferrocene
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/10145 , vital:35360
- Description: Polyamidoamine drug conjugates were prepared from analogues of ferrocene and platinum. Standard reaction procedures were followed in the synthesis of platinum and ferrocene analogues. Michael addition reaction of amines to the activated double bonds of methylenebisacrylamide was applied for preparation of the water soluble polyamidoamine carriers onto which drug analogues were attached. The drug release studies of the conjugates were evaluated at different pH environments. The results obtained from drug release studies showed that rate of drug release was variable depending on the conjugate and pH environment. Mathematical drug release models by Korsmeyer-Peppas were used to determine the drug release characteristics of the ferrocene and platinum based drugs from polyamidoamine drug conjugates. Cytotoxicity potential of the analogues and polyamidoamine drug conjugates was tested on selected cell lines. Cisplatin was used as the standard for comparison of the IC50 values obtained for the compounds tested for cytotoxicity activity. The results from six polymer drug conjugates tested for cytotoxicity activity showed that conjugation of analogues to polyamidoamine carrier enhanced the activity of the analogues in some of the polyamidoamine drug conjugates. Various techniques such as Fourier Transform Infrared (FTIR) spectroscopy, Proton Nuclear Magnetic Resonance spectroscopy (1H NMR), Scanning Electron Microscopy (SEM), transmission electron microscopy (TEM), Transmission Electron Microscopy (TEM) and Energy Dispersive X-ray (EDX) spectroscopy were employed for the characterization of the ferrocene and platinum analogues, polyamidoamine carriers and drug conjugates.
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The influence of technology factors on turnover intentions amongst a sample of government employees in the Eastern Cape, South Africa
- Authors: Mahlasela, Samkelisiwe
- Date: 2018
- Subjects: Work -- Technological innovations
- Language: English
- Type: Thesis , Masters , MCom (Industrial Psychology)
- Identifier: http://hdl.handle.net/10353/13535 , vital:39677
- Description: Orientation: Technology affects organisations both positively and negatively and in some instances technology factors can result in turnover intentions for certain employees of organisations. Research purpose: The main objective of the study was to investigate how technology-based job autonomy, technology-based job overload and technologybased job monitoring impact turnover intentions of employees. Motivation of the study: Exploring and understanding the relationship between technology factors and turnover intention amongst government employees to determine whether these factors affect government as much as they affect the private sector. Research design, approach and method: This research used a quantitative research approach. It drew a convenience sample of 182 employees (N= 182) from the South African Government. Main findings: The three main findings of the study were: Technology-based job autonomy was positively related to turnover intention. Technology-based job overload was found to be positively related to turnover intention. Finally, technology-based monitoring was found to be positively related to turnover intention. Practical/managerial implications: If organisations do not address the issue of monitoring and overload they are likely to experience many turnover intentions amongst the employees. Furthermore, the organisations with less autonomy are likely to lose their employees which means they will lose the resources that have been serving the organisation for a long time. This will also contribute to the organisation losing many funds as they would constantly have to train new employees. Contribution/value-add: The research adds value to the knowledge about the effects of technology factors on turnover intention by pointing out the reasons that lead to turnover intention.
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A discourse analysis of the construction of gendered relationships in grade 10-12 Life Orientation textbooks in the Eastern Cape
- Authors: Adams, Luvo
- Date: 2017
- Subjects: Sex instruction -- South Africa -- Eastern Cape Life skills -- Study and teaching (Secondary)
- Language: English
- Type: Thesis , Masters , MSoc
- Identifier: http://hdl.handle.net/10353/5532 , vital:29315
- Description: School-based sexuality education has been the subject of research in the social sciences and pedagogical spheres globally. In South Africa, growing interest among social scientists in the topic, were ignited by the introduction of sexuality education as a compulsory part of Life Orientation (LO) by the late 1990s. However, the implementation of LO has been problematic. Reviewed literature in the current study, reveals how the dominance of the heterosex discourse is foregrounded in LO content on gender and sexuality. The current study was aimed at examining the construction of gendered relationships in LO textbooks. The study sampled LO textbooks for Grades 10-12, learners in these grades are between the mean ages 16-18 years. This group is the target group, because they are legally afforded the right to consent to sexual activity with peers, within the same age bracket. Conducted from a social constructionist perspective, the current study employed qualitative methods of inquiry (textual analysis). Against the backdrop of heterosexuality as norm, it was the aim of the current study to understand the subject positions made available for female learners to construct themselves, within the discursive spaces in LO content. Findings suggest that two discourses namely: the heterosex discourse and the discourse of danger and disease, dominate in LO content on gender and sexuality. This leads to the construction of gendered relationships a s inherently heterosexual, leading to the marginalisation of relationships that fall outside of the norm. The female learner is positioned as a passive-victim, incapable of exercising sexual agency, while young men are positioned as inherently more powerful members of the intimate relationships or dangerous sexual predators. In the discourse of danger and disease, she is also positioned as a potential victim but the focus is on equipping her with skills, in a way which positioned her as an active-resistor in refusing sexual activity; and being in control of decision-making on issues of safety in relationships. The implications of these contradictions, is that they focus on the individual and disallow her taking up of sexual agency, and disregard the context in which she has to do so.
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Agricultural urbanism and urban agriculture : exploring the possible role of University of Fort Hare and Buffalo City Municipality in supporting small-scale urban farming in East London, South Africa
- Authors: Rumsha, Siphamandla
- Date: 2017
- Subjects: Urban agriculture -- South Africa -- Eastern Cape Agriculture -- Economic aspects -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSoc
- Identifier: http://hdl.handle.net/10353/5072 , vital:29054
- Description: This study investigates the impact of urban agriculture in the city of East London as well as the possibility of adopting agricultural urbanism as an urban farming model to bolster food security. The study goes further to understand the role that could be played by University of Fort Hare and Buffalo City Metropolitan Municipality in capacitating small scale urban farmers in the city of East London. Unemployment and food insecurity are the key challenges that are affecting many families in South Africa, including urban dwellers. The main objective of this study therefore is to investigate the contributions of urban farming in securing food security and livelihoods in East London. This study adopted a mixed methods research approach, where both quantitative and qualitative methods were used to harvest data. Statistical analysis (descriptive) was then used to analyse quantitative data. Thematic approach used to analyse qualitative data. The study shows that urban farming plays an important role in the livelihoods of urban dwellers. However, the sector still faces various challenges such as lack of recognition by authorities. The study also revealed that the city of East London has potential to establish agricultural urbanism as it has most of the essential aspects that are necessary to establish it. The study recommends strengthening of the partnership between University of Fort Hare, Buffalo City Metropolitan Municipality and small scale urban farmers in East London.
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The impact of inflation on financial development in South Africa
- Authors: Muzvanya, Kudzai Fungai
- Date: 2016
- Subjects: Economic development Monetary policy Consumer price indexes
- Language: English
- Type: Thesis , Masters , MCom
- Identifier: http://hdl.handle.net/10353/13045 , vital:39440
- Description: Growing theoretical and empirical studies have predicted different influences that inflation has on financial development in different economies. This dissertation observes the impact South Africa’s inflation has on financial development over the period between 1990 and 2012. Monetary policy framework in South Africa has, to a greater extent, assisted in monitoring the movement of the consumer price index. Although inflation does affect financial sector performance, the study also looked into other variables that have an effect like private credit, money supply and gross domestic product. To test for stationarity to avoid spurious regression, the ADF test and the PP test were used. To determine the long- and short-run relationship, the Johansen Maximum Likelihood test and VECM models were used. Results of the study indicated that money supply and inflation have a negative effect on financial development. In addition, apart from money supply and inflation the findings revealed that private credit and gross domestic product play a significant part in financial sector performance. The study recommends that the South African Reserve Bank should keep the inflation rate within its target range (3-6percent). This would ensure price stability and restore investor confidence in the financial sector, which then improves financial sector development.
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The impact of monetary policy announcement on financial markets in South Africa
- Authors: Chipfunde, Memory
- Date: 2016
- Subjects: Johannesburg Stock Exchange Monetary policy Money market
- Language: English
- Type: Thesis , Masters , MCom
- Identifier: http://hdl.handle.net/10353/12193 , vital:39195
- Description: Monetary policy announcements are among the major decisions that affect the economy as a whole. The purpose of this study was to analyze the impact of monetary policy announcements on equity markets in South Africa during 2014-2015 financial years. That financial period is a unique year for analyzing the impact of monetary announcements on stock returns because the Reserve Bank Governor was changed in the last quarter of 2014. Moreover, interest rates were changed in four monetary policy announcements over the course of two years. This provides a good opportunity to investigate how monetary policy announcements can affect financial markets. The study used an event study methodology whereby a 15-day event window is used as well as a 45-day estimation window. Around the event window, abnormal returns were calculated using the market model and capital asset pricing model (CAPM) was used to calculate expected returns. The results are mixed; (both significant and insignificant) impact of monetary policy announcements on JSE stock returns. Of the 12 interest rates announcements, only four seemed to be significant. It was observed that of the four significant results, three of them were because of a hike in interest rates. The results show an asymmetric response by the market to interest rate changes. The results also suggest that the market anticipated the relative volatility arising from the change of the minister of the reserve bank governor. Following the appointment of the new Central Bank‟s governor in the last quarter of 2014, the first interest rate announcement seemed to have been anticipated by the public thereby rendering it insignificant. Inorder to improve the strength of the results in future, it is important that that there should be the absence of signalling effect in the market. It is recommended that the markets are efficient and the announcements should not be highly anticipated by the public.
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The relationship between household debt and consumption spending in South Africa (1994 - 2013)
- Authors: Nkala, Patience
- Date: 2016
- Subjects: Finance, Personal Financial services industry Consumption (Economics)
- Language: English
- Type: Thesis , Masters , MCom
- Identifier: http://hdl.handle.net/10353/14022 , vital:39798
- Description: Consumption has been and remains the main contributor to gross domestic product (GDP) growth in South Africa. Household debt on the other side has remained high over the years. This study examined the relationship between household debt and consumption spending, for the period between 1994 and 2013. The Johansen cointegration technique and the Vector error correction model (VECM) were utilised to test the long run and short run relationships between the variables. The Granger causality test was also employed to test the direction of causality between the variables. Results from this study have revealed that a relationship exists between household debt and consumption spending in South Africa and they have also showed that this relationship flows from household debt to consumption spending. The implications of these results are that consumption spending may be increased through other measures rather than through increasing debt. The study therefore recommends that policy makers avail more investment opportunities for households and to also create employment in a bid to increase the income of households which can then be used to increase household consumption rather than the use of debt.
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Risk management : a prerequisite for the implementation of government projects by the Eastern Cape Department of Local Government
- Authors: Sojini, Sandile
- Date: 2015
- Subjects: Risk management -- South Africa -- Eastern Cape Project management -- South Africa -- Eastern Cape Local government -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MPA
- Identifier: http://hdl.handle.net/10353/2755 , vital:28073
- Description: This Research Report that this research was motivated by the fact that despite thetremendous achievements have been realized from the successful implementation ofgovernment programmes and projects related the construction and refurbishment of medical facilities, educational, bridges and road infrastructure facilities. Trends indicate that only iota of evidence implies that the concept of risk management is fully integrated and embraced as part of a culture for ensuring the successful project implementation by the Modern South African public sector organizations. Empirical research revealed that the common types of risks that have been affecting the successful implementation of government projects and programmes include: riots, fraud, corruption, changes in demographic figures, outsourcing risks, and floods and changes in weather. Most of the participants stated that risks associated with riots do not only arise from the riots within the government departments, but also in the external environment. In terms of the internal riots, they revealed that when the employees go on strike or riots on their own or in solidarity with the other trade unions, leading to the process for the implementation of government projects and programmes being affected. Yet, in certain cases, they noted that internal riots or strikes most occur as preceded by rumours that the riots by a particular union will take place and may significantly affect the ongoing process of implementation of government programmes. Besides risks associated with the internal riots by the employees and the communities, the interview findings also indicated that the other forms of risks arise from the strikes and riots by the private enterprises.
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Studies on bioflocculants produced by three freshwater Actinomycetes (Streptomyces Sp.Gansen, Cellulomonas Sp,Bola and Brachybacterium Sp, UFH) isolated from Tyume river
- Authors: Oladele, Agunbiade M
- Date: 2011
- Subjects: Flocculation Streptomyces Gram-positive bacteria Actinobacteria
- Language: English
- Type: Thesis , Masters , Degree
- Identifier: http://hdl.handle.net/10353/6550 , vital:30552
- Description: Several bacteria were isolated from the bottom sediments of Tyume River and investigated for bioflocculant production potentials. Kaolin clay suspension (4 g/l) was used to measure the flocculating activity and three of the positive isolates were identified by 16S rRNA gene nucleotide sequence analyses and the sequences deposited in GenBank as Streptomyces sp Gansen (accession number HQ537129), Brachybacterium sp UFH (accession number HQ537131.), and Cellulomonas sp Bola (accession number HQ537132). Streptomyces sp Gansen exhibited its maximum flocculating activity using lactose (85% activity), peptone (76.3% activity), Ca2+ as sole sources of carbon, nitrogen and cations respectively, and at a neutral pH of 7.0, while, the bioflocculant produced by Brachybacterium sp UFH with glucose, urea and Ca2+ as carbon, nitrogen and cations sources yielded 82% and 97% flocculation activity respectively at a neutral pH. Also, glucose (73.2% activity), ammonium chloride (78.2% activity) and Ca2+ resulted in optimal production of bioflocculant by Cellulomonas sp Bola, also at a neutral pH. Chemical analysis confirmed that bioflocculant produced by Streptomyces Gansen is a polysaccharide while Brachybacterium sp UFH and Cellulomonas sp Bola produces a glycoprotein compound. This freshwater actinomycetes appears to have a tremendous potential as sou rces of new bioflocculants.
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