An investigation of the contribution of street vending on livelihoods : case of street vendors in Nkonkobe Municipality
- Authors: Dube, Thulani
- Date: 2017
- Subjects: Street vendors -- South Africa -- Eastern Cape Street vendors -- South Africa -- Eastern Cape -- Economic conditions Vending stands -- Economic aspects -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSoc
- Identifier: http://hdl.handle.net/10353/6068 , vital:29488
- Description: The informal sector thrives in a context of high unemployment, underemployment, poverty, gender inequality and precarious work. It plays a significant role in such circumstances, especially in income generation and in contributing to the livelihood of those engaged in it. The study investigated the contribution of street vending to the livelihood of street vendors in Nkonkobe Municipality. The aim of the study was to investigate whether street vending has the potential to sustain the lives of those engaged in it. In order to reach its objectives, the study employed a qualitative research approach whereby a non-probability sampling design in the form of a purposive sampling method was adopted and considered appropriate for this study. In terms of data collection, the study made use of structured questionnaires as a data collection method whereby questionnaires were administered and distributed to 40 street venders. The Upper Bound Poverty Line (UBPL) was used as a measurement to assess whether the participants’ incomes were above the poverty datum line in order to determine the sector’s contribution to the traders’ livelihood. From this study, it was found that street vending has both positive and negative impacts with regard to the success and profit-earning potential of these traders. Thus, the study concluded that street vending is a vital community development tool and a major source of livelihood as it plays a very important socio-economic role in Nkonkobe and in South Africa in general. The sector provides a livelihood for a huge population mainly comprised of the poor and other impoverished groups of society.
- Full Text:
- Authors: Dube, Thulani
- Date: 2017
- Subjects: Street vendors -- South Africa -- Eastern Cape Street vendors -- South Africa -- Eastern Cape -- Economic conditions Vending stands -- Economic aspects -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSoc
- Identifier: http://hdl.handle.net/10353/6068 , vital:29488
- Description: The informal sector thrives in a context of high unemployment, underemployment, poverty, gender inequality and precarious work. It plays a significant role in such circumstances, especially in income generation and in contributing to the livelihood of those engaged in it. The study investigated the contribution of street vending to the livelihood of street vendors in Nkonkobe Municipality. The aim of the study was to investigate whether street vending has the potential to sustain the lives of those engaged in it. In order to reach its objectives, the study employed a qualitative research approach whereby a non-probability sampling design in the form of a purposive sampling method was adopted and considered appropriate for this study. In terms of data collection, the study made use of structured questionnaires as a data collection method whereby questionnaires were administered and distributed to 40 street venders. The Upper Bound Poverty Line (UBPL) was used as a measurement to assess whether the participants’ incomes were above the poverty datum line in order to determine the sector’s contribution to the traders’ livelihood. From this study, it was found that street vending has both positive and negative impacts with regard to the success and profit-earning potential of these traders. Thus, the study concluded that street vending is a vital community development tool and a major source of livelihood as it plays a very important socio-economic role in Nkonkobe and in South Africa in general. The sector provides a livelihood for a huge population mainly comprised of the poor and other impoverished groups of society.
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Differential regulation of wheat (Triticum aestivum) callose synthases during Russian wheat aphid (diuraphis noxia)infestation
- Authors: Ngwenya, Nompilo
- Date: 2017
- Subjects: Russian wheat aphid Wheat -- Diseases and pests
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/8100 , vital:31522
- Description: Plant production serves as the primary food source for all living beings and is continuously being affected by many biotic and abiotic factors. Wheat, one of the most consumed cereal crops in the world and the second most cultivated crop after maize in South Africa, is continuously being affected by Russian wheat aphid (RWA) since 1978. To counteract the effect of this aphid, the first resistant wheat cultivar was developed in 1984 in South Africa containing the Dn1 resistance gene. However, the mechanism of action of the resistance is still not fully understood to date. The feeding of the RWA on the wheat induces callose deposition especially in susceptible wheat cultivars, linking the β-1.3-polysaccharide (callose) to the resistance mechanism. Callose is synthesized by callose synthases, also known as glucan synthase-like genes (GSL). Not much is known about the mechanism of action of callose synthases or how they are regulated in response to the RWA. Bioinformatics tools, such as those available at NCBI, were used to identify the wheat callose synthases genes. The proteins encoded by the identified wheat callose synthases genes, were then analyzed for conserved domain motifs using the MEME suite and InterproScan, sequence similarities using ClustaL Omega and transmembrane domains using HMMTOP and ProtCamp. These were then compared with those of the already characterized Arabidopsis callose synthase proteins in an attempt to identify the wheat callose synthase(s) that responds to aphid feeding. Further bioinformatics studies were carried out to identify the presence of biotic stress associated cis-acting regulatory elements found in the 1.5 kbp upstream region of the start codon of the Arabidopsis callose synthase genes. Eight partial wheat callose synthase sequences were identified and two of these (TaGSL2 and TaGSL22) showed high similarities to the AtGSL5, which is up-regulated in response to aphid feeding in Arabidopsis. Six of the wheat callose synthase genes were mediated to code for the functional callose synthases proteins: TaGSL3, 8, 12, 19, 22 and 23, and analyzed for conserved protein motifs. Based on the sequence similarities and conserved protein domains, TaGSL2 and TaGSL22 were found to be the most similar to AtGSL5 and most likely to respond to RWA infestation. Cis-acting regulatory element analyses confirmed the possibility of TaGSL22 being responsible for callose deposition in wheat as biotic stress associated cis-acting regulatory elements, Box W1, TC- rich element and W- box were all found in the 1.5 kbp upstream of the TaGSL22 coding region. Callose was quantified in both susceptible and resistant wheat cultivars using the aniline blue fluorescence method. When infested with RWA-SA1, the susceptible wheat cultivar (Tugela) deposited significantly higher amounts of callose, compared to the resistant wheat cultivar (Tugela DN), which deposited little, to no callose with respect to the control samples. Transcription analysis, of the TaGSL2 and TaGSL22 in RWA-SA2 infested Tugela and Tugela DN wheat cultivars, was performed using real time polymerase chain reaction (qPCR). Both TaGSL2 and TaGSL22 genes were up-regulated in Tugela and Tugela DN wheat cultivars in response to RWA-SA2 infestation, with TaGSL22 being more expressed than TaGSL2 in both cultivars, indicating that RWA-SA2 is able to overcome the resistance of Tugela DN and cause callose deposition. However, significantly higher expression of both genes was still observed in the susceptible, Tugela wheat cultivar. This study therefore confirms that callose deposition is associated with RWA-SA1 feeding, only in the susceptible wheat cultivar, Tugela and not the resistant cultivar, Tugela DN. However, during RWA-SA2 feeding, two of the eight identified callose synthases in wheat, are up-regulated in response to RWA-SA2 feeding, in both the resistant and susceptible wheat cultivars with higher levels observed in the susceptible wheat cultivar when compared to the resistant wheat cultivar. TaGSL2 and TaGSL22 are therefore implicated in the callose deposition observed in the susceptible and resistant wheat cultivars, after RWA-SA2 infestation. Further studies are required to confirm the differential regulation of the two wheat callose synthases proteins during RWA infestation and their possible role in the resistance mechanism of the resistant wheat cultivar, Tugela DN.
- Full Text:
- Authors: Ngwenya, Nompilo
- Date: 2017
- Subjects: Russian wheat aphid Wheat -- Diseases and pests
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/8100 , vital:31522
- Description: Plant production serves as the primary food source for all living beings and is continuously being affected by many biotic and abiotic factors. Wheat, one of the most consumed cereal crops in the world and the second most cultivated crop after maize in South Africa, is continuously being affected by Russian wheat aphid (RWA) since 1978. To counteract the effect of this aphid, the first resistant wheat cultivar was developed in 1984 in South Africa containing the Dn1 resistance gene. However, the mechanism of action of the resistance is still not fully understood to date. The feeding of the RWA on the wheat induces callose deposition especially in susceptible wheat cultivars, linking the β-1.3-polysaccharide (callose) to the resistance mechanism. Callose is synthesized by callose synthases, also known as glucan synthase-like genes (GSL). Not much is known about the mechanism of action of callose synthases or how they are regulated in response to the RWA. Bioinformatics tools, such as those available at NCBI, were used to identify the wheat callose synthases genes. The proteins encoded by the identified wheat callose synthases genes, were then analyzed for conserved domain motifs using the MEME suite and InterproScan, sequence similarities using ClustaL Omega and transmembrane domains using HMMTOP and ProtCamp. These were then compared with those of the already characterized Arabidopsis callose synthase proteins in an attempt to identify the wheat callose synthase(s) that responds to aphid feeding. Further bioinformatics studies were carried out to identify the presence of biotic stress associated cis-acting regulatory elements found in the 1.5 kbp upstream region of the start codon of the Arabidopsis callose synthase genes. Eight partial wheat callose synthase sequences were identified and two of these (TaGSL2 and TaGSL22) showed high similarities to the AtGSL5, which is up-regulated in response to aphid feeding in Arabidopsis. Six of the wheat callose synthase genes were mediated to code for the functional callose synthases proteins: TaGSL3, 8, 12, 19, 22 and 23, and analyzed for conserved protein motifs. Based on the sequence similarities and conserved protein domains, TaGSL2 and TaGSL22 were found to be the most similar to AtGSL5 and most likely to respond to RWA infestation. Cis-acting regulatory element analyses confirmed the possibility of TaGSL22 being responsible for callose deposition in wheat as biotic stress associated cis-acting regulatory elements, Box W1, TC- rich element and W- box were all found in the 1.5 kbp upstream of the TaGSL22 coding region. Callose was quantified in both susceptible and resistant wheat cultivars using the aniline blue fluorescence method. When infested with RWA-SA1, the susceptible wheat cultivar (Tugela) deposited significantly higher amounts of callose, compared to the resistant wheat cultivar (Tugela DN), which deposited little, to no callose with respect to the control samples. Transcription analysis, of the TaGSL2 and TaGSL22 in RWA-SA2 infested Tugela and Tugela DN wheat cultivars, was performed using real time polymerase chain reaction (qPCR). Both TaGSL2 and TaGSL22 genes were up-regulated in Tugela and Tugela DN wheat cultivars in response to RWA-SA2 infestation, with TaGSL22 being more expressed than TaGSL2 in both cultivars, indicating that RWA-SA2 is able to overcome the resistance of Tugela DN and cause callose deposition. However, significantly higher expression of both genes was still observed in the susceptible, Tugela wheat cultivar. This study therefore confirms that callose deposition is associated with RWA-SA1 feeding, only in the susceptible wheat cultivar, Tugela and not the resistant cultivar, Tugela DN. However, during RWA-SA2 feeding, two of the eight identified callose synthases in wheat, are up-regulated in response to RWA-SA2 feeding, in both the resistant and susceptible wheat cultivars with higher levels observed in the susceptible wheat cultivar when compared to the resistant wheat cultivar. TaGSL2 and TaGSL22 are therefore implicated in the callose deposition observed in the susceptible and resistant wheat cultivars, after RWA-SA2 infestation. Further studies are required to confirm the differential regulation of the two wheat callose synthases proteins during RWA infestation and their possible role in the resistance mechanism of the resistant wheat cultivar, Tugela DN.
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Species composition and seasonal population dynamics of free-living and engorged cattle ticks in three agro-ecological zones of the Eastern Cape Province, South Africa
- Authors: Yawa, Mandla
- Date: 2017
- Subjects: Ticks Boophilus microplus
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/9067 , vital:34224
- Description: This study was conducted to determine species composition and seasonal population dynamics of free-living and engorged cattle ticks in three agro-ecological zones (Bedford dry grassland, Kowie thicket and Bisho thornveld) of the Eastern Cape Province, South Africa. The occurrence of ticks on cattle was studied monthly from April 2016 and March 2017. Tick collections were performed on 360 selected cattle in each agro-ecological zones. During tick collections, 10 randomly selected cattle were sampled at each locality and more attention was given to African blue tick and the presence of Asian blue tick. A half body tick collection was performed from cattle and visible ticks were collected by means of fine-forceps after the animals had been restrained. Body regions that were examined includes the ear, head, neck, chest, abdomen, flank, front and hind leg and feet, tail, and peri-anal region. At each locality, six replicate drags of 100 m, approximately 50 m apart, were performed to collect ticks from vegetation. All ticks collected per cattle and from vegetation were stored in labelled sample tubes containing 70percent ethanol, subsequently counted and identified to genus and species level using a standard stereomicroscope. A total of 31425 ticks belonging to ten species were collected during the study. Rhipicephalus (Boophilus) decoloratus, was the most observed tick species (32.50percent), all other ticks like R. evertsi evertsi (18.84percent), R. appendiculatus (17.26percent), A. hebraeum (16.27percent), R. simus (7.69percent) were also commonly collected. Ixodes pilosus (3.84percent), H. rufipes (3.46percent), R. follis (0.08percent) together with Haemaphysalis silacea (0.02percent) were sporadic infestation. Haemaphysalis elliptica (0.04percent) was only found on the vegetation. No H. rufipes was collected in the thicket vegetation. Agro-ecological zones differ significantly (P<0.05) in tick species and distribution. Significantly more R. (B.) decoloratus were collected in thicket during summer compared to thornveld and dry grassland regions (P<0.05). Free-living ticks were widely distributed across different seasons but with of R. (B.) decoloratus larvae were significantly higher (P<0.05) in thornveld region during the spring season. In conclusion, agro-ecological differences and seasonal variations had an influence on ticks abundance and distribution. From this study, it is of interest to note the absence of R. (B.) microplus, whose trend to invade new areas previously colonised by R. (B.) decoloratus has been observed in Africa. In conclusion, agro-ecological differences and seasonal variations had an influence on ticks abundance and distribution. The aforementioned absence of R. (B.) microplus in this study highlights the importance of further research as this tick invades new areas previously colonised by R. (B.) decoloratus in the continent.
- Full Text:
- Authors: Yawa, Mandla
- Date: 2017
- Subjects: Ticks Boophilus microplus
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/9067 , vital:34224
- Description: This study was conducted to determine species composition and seasonal population dynamics of free-living and engorged cattle ticks in three agro-ecological zones (Bedford dry grassland, Kowie thicket and Bisho thornveld) of the Eastern Cape Province, South Africa. The occurrence of ticks on cattle was studied monthly from April 2016 and March 2017. Tick collections were performed on 360 selected cattle in each agro-ecological zones. During tick collections, 10 randomly selected cattle were sampled at each locality and more attention was given to African blue tick and the presence of Asian blue tick. A half body tick collection was performed from cattle and visible ticks were collected by means of fine-forceps after the animals had been restrained. Body regions that were examined includes the ear, head, neck, chest, abdomen, flank, front and hind leg and feet, tail, and peri-anal region. At each locality, six replicate drags of 100 m, approximately 50 m apart, were performed to collect ticks from vegetation. All ticks collected per cattle and from vegetation were stored in labelled sample tubes containing 70percent ethanol, subsequently counted and identified to genus and species level using a standard stereomicroscope. A total of 31425 ticks belonging to ten species were collected during the study. Rhipicephalus (Boophilus) decoloratus, was the most observed tick species (32.50percent), all other ticks like R. evertsi evertsi (18.84percent), R. appendiculatus (17.26percent), A. hebraeum (16.27percent), R. simus (7.69percent) were also commonly collected. Ixodes pilosus (3.84percent), H. rufipes (3.46percent), R. follis (0.08percent) together with Haemaphysalis silacea (0.02percent) were sporadic infestation. Haemaphysalis elliptica (0.04percent) was only found on the vegetation. No H. rufipes was collected in the thicket vegetation. Agro-ecological zones differ significantly (P<0.05) in tick species and distribution. Significantly more R. (B.) decoloratus were collected in thicket during summer compared to thornveld and dry grassland regions (P<0.05). Free-living ticks were widely distributed across different seasons but with of R. (B.) decoloratus larvae were significantly higher (P<0.05) in thornveld region during the spring season. In conclusion, agro-ecological differences and seasonal variations had an influence on ticks abundance and distribution. From this study, it is of interest to note the absence of R. (B.) microplus, whose trend to invade new areas previously colonised by R. (B.) decoloratus has been observed in Africa. In conclusion, agro-ecological differences and seasonal variations had an influence on ticks abundance and distribution. The aforementioned absence of R. (B.) microplus in this study highlights the importance of further research as this tick invades new areas previously colonised by R. (B.) decoloratus in the continent.
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The experiences of young adults raised in single-parent families in the Raymond Mhlaba Local Municipality, Eastern Cape, South Africa
- Authors: Makoni, Mavis
- Date: 2017
- Subjects: Single-parent families , Children of single parents Young adults
- Language: English
- Type: Thesis , Masters , MSoc
- Identifier: http://hdl.handle.net/10353/5292 , vital:29184
- Description: Although the single-parent family is becoming an increasingly common family structure, it is associated with a great many hardships. Owing to the heavy responsibilities which fall on single parents, most fail to meet all of the needs of their children, particularly with respect to the financial needs which are entailed by providing educational necessities, safe, stable and permanent accommodation, adequate food and nutrition and healthcare. This study adopted the parental loss perspective, the economic hardship perspective and the interparental conflict perspective in investigating the experiences of young adults who were raised in single-parent families. A qualitative approach was adopted to conduct the research and data was collected from 26 male and female participants between the ages of 22 and 39 years who resided within the Raymond Mhlaba Local Municipality. The researcher sought permission to conduct this study from all the community leaders including the councillors and chiefs, thereby acquiring the first referrals. The study made use of purposive sampling and snowballing to select the research sample. The data was collected by using semi-structured interviews and focus group discussions and analysed through the use of thematic analysis. The principal findings of the study indicate that although children who are raised in single-parent homes can attain high levels of education and have solid functional relationships in later life, a lack of finance and active parental involvement in their educational activities and social lives can serve to limit their development. As single-mother households face more economic hardships than any other family structures, financial assistance from both the government and their communities would undoubtedly increase the likelihood of children in single-mother families attaining their true academic potential. In addition, free counselling services would also help children who are forced to live under these circumstances overcome the emotional burden which inevitably accompanies either losing or being separated from one parent.
- Full Text:
- Authors: Makoni, Mavis
- Date: 2017
- Subjects: Single-parent families , Children of single parents Young adults
- Language: English
- Type: Thesis , Masters , MSoc
- Identifier: http://hdl.handle.net/10353/5292 , vital:29184
- Description: Although the single-parent family is becoming an increasingly common family structure, it is associated with a great many hardships. Owing to the heavy responsibilities which fall on single parents, most fail to meet all of the needs of their children, particularly with respect to the financial needs which are entailed by providing educational necessities, safe, stable and permanent accommodation, adequate food and nutrition and healthcare. This study adopted the parental loss perspective, the economic hardship perspective and the interparental conflict perspective in investigating the experiences of young adults who were raised in single-parent families. A qualitative approach was adopted to conduct the research and data was collected from 26 male and female participants between the ages of 22 and 39 years who resided within the Raymond Mhlaba Local Municipality. The researcher sought permission to conduct this study from all the community leaders including the councillors and chiefs, thereby acquiring the first referrals. The study made use of purposive sampling and snowballing to select the research sample. The data was collected by using semi-structured interviews and focus group discussions and analysed through the use of thematic analysis. The principal findings of the study indicate that although children who are raised in single-parent homes can attain high levels of education and have solid functional relationships in later life, a lack of finance and active parental involvement in their educational activities and social lives can serve to limit their development. As single-mother households face more economic hardships than any other family structures, financial assistance from both the government and their communities would undoubtedly increase the likelihood of children in single-mother families attaining their true academic potential. In addition, free counselling services would also help children who are forced to live under these circumstances overcome the emotional burden which inevitably accompanies either losing or being separated from one parent.
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