Isolation, characterisation of terpenoids and biosynthesis of silver nanoparticles of acacia mearnsii de wild and acacia Karroo Hayne and their Bioassays
- Authors: Avoseh, Opeyemi Nudewhenu
- Date: 2015
- Language: English
- Type: Thesis , Doctoral , PhD (Chemistry)
- Identifier: vital:11355 , http://hdl.handle.net/10353/d1021292
- Description: Great wealth of traditional knowledge about the use of plants had been transferred from generation to generations leading to the present day drug discovery and invention of new scientific methods of isolation, purification and identification. With the discovery of new diseases and drug-resistant organisms, there is no other source or deposit of lead compounds or drugs than the plant kingdom. As a result of this, about 25% of the current drug administered owe their origin to plant sources with the view to reduce the carcinogenic effect of synthetic drugs. Volatile terpenoids among other broad spectrum of natural product had been implicated to show high therapeutic properly. In the present study, selected locally-used medicinal plants were exploited for the presence of potent bioactive compounds and ability to form nanoparticles with distinctive property for use as chemoprotective agent against inflammation, tumors, cancer and other chronic diseases. Acacia mearnsii De Wild and Acacia karroo Hayne studied in this report are known to be invasive species with no proper regulation to conserve and preserve them. However, ethnopharmacology report of these plant species in the Southern Africa region reveals that they are good antiseptic, anti-diarrhea, anti-inflammation and a forage for livestock. These plants were subjected to volatile extraction protocol of some parts of the plants (stem and leaves) followed by examination of the anti-inflammation capacity of the extracts using an animal model. In addition, the bye-product (hydrosol) from the stem bark of each species possess a high reducing and stabilizing property leading to synthesis of silver nanoparticles, followed by investigation of the anti-inflammation potential of the synthesized silver nanoparticles using animal model. The volatile oils of the leaves and stem bark of Acacia mearnsii De Wild obtained by hydro-distillation were analyzed by Gas Chromatography-Mass Spectrometry (GC-MS). Twenty, Thirty-Eight, Twenty-nine and Thirty-Eight components accounting for 93.8%, 92.1%, 78.5% and 90.9% of the total oils of the fresh, dry leaves and fresh, dry stem bark respectively. The major components of the oil were octadecyl alcohol (25.5%) and phytol (10.5%); cis-verbenol (29.5%); phytol (10.1%) and phytol (23.4%) for the fresh leaves, dried leaves, and fresh stem, dry stem bark respectively. Oral administration of essential oils at the dose of 2% showed significant (p<0.05) anti-inflammatory properties in the albumin induced test model in rats. Oils from the fresh leaves and dry stems inhibited inflammation beyond 4 h post treatment. Furthermore, the chemical composition of the essential oils obtained by hydro-distillation from the leaves and stem bark (dry and fresh) of Acacia karroo Hayne, analysed by GC-MS, shows that hexanal (10.67%) and ß-ionone (9.74%) were dominant in the dried leaves, β-pinene (14.30%), and (Z)-2-Hexen-1-ol (10.21%) in the fresh leaves while Octacosane (10.59%) and phytol (23.38%) were dominant in the dry and fresh stem respectively. The anti-inflammation ability of these oils after an albumin-induced inflammation on wistar rats, shows a significant effect at the 1st h of treatment with a significance of P< 0.01 for all part plants, while the fresh leaves shows further inhibitory activities at the 2nd h of analysis. Silver nanoparticles (AgNPs) were successfully synthesized from AgNO3 through a green route using the aqueous extract (hydrosols) of Acacia mearnsii De Wild and Acacia karroo Hayne as reducing agent and as well as capping agent. The Acacia-mediated AgNPs were characterized with the use of UV-vis absorption spectroscopy, Fourier Transform Spectroscopy (FT-IR), Transmission electron microscope (TEM), Scanning Electron Microscope (SEM), Energy Dispersive Spectroscopy (EDX), and X-ray Diffractometry (XRD). A spherical, 10-40 nm diameter silver nanoparticles were synthesized with very low level of stability for the AMDS and the AKDS-AgNPs. In addition, nociceptive activity with a mice rat reveals higher inhibition at the neurogenic phase for the AKDS-AgNPs, while AMDS-AgNPs exhibited a high inhibition at the inflammatory phase. The potent anti-inflammatory activity of essential oils of A. mearnsii De Wild and A. karroo Hayne hereby confirmed its traditional use in treating various inflammatory diseases, while the inflammatory studies on the synthesized AgNPs reveals a very active compound which can be used as a potent opioid or non-steroidal anti-inflammatory drug (NSAID).
- Full Text:
- Authors: Avoseh, Opeyemi Nudewhenu
- Date: 2015
- Language: English
- Type: Thesis , Doctoral , PhD (Chemistry)
- Identifier: vital:11355 , http://hdl.handle.net/10353/d1021292
- Description: Great wealth of traditional knowledge about the use of plants had been transferred from generation to generations leading to the present day drug discovery and invention of new scientific methods of isolation, purification and identification. With the discovery of new diseases and drug-resistant organisms, there is no other source or deposit of lead compounds or drugs than the plant kingdom. As a result of this, about 25% of the current drug administered owe their origin to plant sources with the view to reduce the carcinogenic effect of synthetic drugs. Volatile terpenoids among other broad spectrum of natural product had been implicated to show high therapeutic properly. In the present study, selected locally-used medicinal plants were exploited for the presence of potent bioactive compounds and ability to form nanoparticles with distinctive property for use as chemoprotective agent against inflammation, tumors, cancer and other chronic diseases. Acacia mearnsii De Wild and Acacia karroo Hayne studied in this report are known to be invasive species with no proper regulation to conserve and preserve them. However, ethnopharmacology report of these plant species in the Southern Africa region reveals that they are good antiseptic, anti-diarrhea, anti-inflammation and a forage for livestock. These plants were subjected to volatile extraction protocol of some parts of the plants (stem and leaves) followed by examination of the anti-inflammation capacity of the extracts using an animal model. In addition, the bye-product (hydrosol) from the stem bark of each species possess a high reducing and stabilizing property leading to synthesis of silver nanoparticles, followed by investigation of the anti-inflammation potential of the synthesized silver nanoparticles using animal model. The volatile oils of the leaves and stem bark of Acacia mearnsii De Wild obtained by hydro-distillation were analyzed by Gas Chromatography-Mass Spectrometry (GC-MS). Twenty, Thirty-Eight, Twenty-nine and Thirty-Eight components accounting for 93.8%, 92.1%, 78.5% and 90.9% of the total oils of the fresh, dry leaves and fresh, dry stem bark respectively. The major components of the oil were octadecyl alcohol (25.5%) and phytol (10.5%); cis-verbenol (29.5%); phytol (10.1%) and phytol (23.4%) for the fresh leaves, dried leaves, and fresh stem, dry stem bark respectively. Oral administration of essential oils at the dose of 2% showed significant (p<0.05) anti-inflammatory properties in the albumin induced test model in rats. Oils from the fresh leaves and dry stems inhibited inflammation beyond 4 h post treatment. Furthermore, the chemical composition of the essential oils obtained by hydro-distillation from the leaves and stem bark (dry and fresh) of Acacia karroo Hayne, analysed by GC-MS, shows that hexanal (10.67%) and ß-ionone (9.74%) were dominant in the dried leaves, β-pinene (14.30%), and (Z)-2-Hexen-1-ol (10.21%) in the fresh leaves while Octacosane (10.59%) and phytol (23.38%) were dominant in the dry and fresh stem respectively. The anti-inflammation ability of these oils after an albumin-induced inflammation on wistar rats, shows a significant effect at the 1st h of treatment with a significance of P< 0.01 for all part plants, while the fresh leaves shows further inhibitory activities at the 2nd h of analysis. Silver nanoparticles (AgNPs) were successfully synthesized from AgNO3 through a green route using the aqueous extract (hydrosols) of Acacia mearnsii De Wild and Acacia karroo Hayne as reducing agent and as well as capping agent. The Acacia-mediated AgNPs were characterized with the use of UV-vis absorption spectroscopy, Fourier Transform Spectroscopy (FT-IR), Transmission electron microscope (TEM), Scanning Electron Microscope (SEM), Energy Dispersive Spectroscopy (EDX), and X-ray Diffractometry (XRD). A spherical, 10-40 nm diameter silver nanoparticles were synthesized with very low level of stability for the AMDS and the AKDS-AgNPs. In addition, nociceptive activity with a mice rat reveals higher inhibition at the neurogenic phase for the AKDS-AgNPs, while AMDS-AgNPs exhibited a high inhibition at the inflammatory phase. The potent anti-inflammatory activity of essential oils of A. mearnsii De Wild and A. karroo Hayne hereby confirmed its traditional use in treating various inflammatory diseases, while the inflammatory studies on the synthesized AgNPs reveals a very active compound which can be used as a potent opioid or non-steroidal anti-inflammatory drug (NSAID).
- Full Text:
Constitutional damages for the infringement of a social assistance right in South Africa are monetary damages in the form of interest a just and equitable remedy for breach of a social assistance right
- Authors: Batchelor, Bronwyn Le Ann
- Date: 2011
- Subjects: South Africa -- Constitution -- Bill of Rights , Constitutional law -- South Africa , Human rights -- South Africa , Constitutional history -- South Africa , Social security -- Law and legislation -- South Africa , Civil rights -- South Africa , Common law -- South Africa , Compensation (Law) , Damages
- Language: English
- Type: Thesis , Masters , LLM
- Identifier: vital:11117 , http://hdl.handle.net/10353/388 , South Africa -- Constitution -- Bill of Rights , Constitutional law -- South Africa , Human rights -- South Africa , Constitutional history -- South Africa , Social security -- Law and legislation -- South Africa , Civil rights -- South Africa , Common law -- South Africa , Compensation (Law) , Damages
- Description: This dissertation will explore the revolutionary progression in the provision of monetary damages and the availability thereof due to the change in South Africa’s legal system from Parliamentary sovereignty to Constitutional supremacy after the enactment of the final Constitution in 19961. The Constitution of South Africa brought with it the concepts of justification and accountability as the Bill of Rights enshrines fundamental rights and the remedies for the infringement of same. The available remedies for the infringement of a fundamental right flow from two sources, being either from the development of the common law remedies in line with the Bill of Rights or alternatively from Section 38 of the Constitution, which provides for a remedy which provides ‘appropriate’ relief. The question that will be raised in this dissertation is, ‘does appropriate relief include an award of delictual damages?’ or a question related thereto ‘is an award of monetary damages an appropriate remedy?’ The motivation for this dissertation arises from the plethora of case law, especially in the Eastern Cape, that has come to the fore in the last sixteen years, highlighting the injustice of cancellations of social assistance grants and the non-payment of such in South Africa’s social security system, as well as the precedent that was set by our Constitutional Court and Supreme Court in remedying that injustice. The central case to this dissertation is that of Kate v Member of Executive Council for Department of Welfare, Eastern Cape 2005 1 SA 141 SECLD; Member of Executive Council, Department of Welfare, Eastern Cape v Kate 2006 (4) SA 478 (SCA), which is generally regarded as having paved the way for the granting of monetary damages for the infringement of an individual’s constitutional right as same require legal protection. Firstly the past approach to damages will be explored in relation to South Africa’s common law, being the Roman-Dutch law. The common law Aquilian action is the focal point of this dissertation in relation to the common law in that the granting of damages for the infringement of an individual’s social assistance right (being a specific constitutional right framed within the 1996 Constitution) results in pure patrimonial loss which in our common law system was remedied by the actio legis Aquilae. In delict, an award of damages is the primary remedy, aimed at affording compensation in respect of the legal right or interest infringed. After the common law system of damages has been explored, this dissertation will then examine the changes that have developed therefrom, and largely shaped by the current state of disorganization in the National Department of Welfare coupled with the all encompassing power of the final Constitution. The final Constitution provides the power, in section 38 of the 1996 Constitution, for the court to award a monetary remedy for the breach of a constitutional right. The question, however, is “does the award of monetary damages not merely throw money at the problem, whereas the purpose of a constitutional remedy is to vindicate guaranteed rights and prevent or deter future violations?” The battle for domination between the common law approach and the constitutional approach to damages is witnessed as the two systems eventually amalgamate to form an essentially new remedy, unique to South Africa. South Africa’s new system is aligned with the Constitution as the Constitution is the supreme law of the land and underpins the awarding of all damages and, especially, the awarding of constitutional damages. For the sake of completeness, alternatives to monetary damages will also be canvassed in this dissertation. It is hoped that the reader will, in the end, realize that the final Constitution is the supreme law of the land and as such dictates the manner and form in which damages are provided. If such provision is not in alignment with the Constitution, it will be declared invalid. The flexibility of our common law is put to the test, yet it is found to be adaptable to the ever-developing boni mores of society exemplified in the embracing constitutional principles and the production of this new remedy. The courts develop the common law under section 39(2) of the Constitution in order to keep the common law in step with the evolution of our society and the ever changing nature of bonos mores. , National Research Foundation
- Full Text:
- Authors: Batchelor, Bronwyn Le Ann
- Date: 2011
- Subjects: South Africa -- Constitution -- Bill of Rights , Constitutional law -- South Africa , Human rights -- South Africa , Constitutional history -- South Africa , Social security -- Law and legislation -- South Africa , Civil rights -- South Africa , Common law -- South Africa , Compensation (Law) , Damages
- Language: English
- Type: Thesis , Masters , LLM
- Identifier: vital:11117 , http://hdl.handle.net/10353/388 , South Africa -- Constitution -- Bill of Rights , Constitutional law -- South Africa , Human rights -- South Africa , Constitutional history -- South Africa , Social security -- Law and legislation -- South Africa , Civil rights -- South Africa , Common law -- South Africa , Compensation (Law) , Damages
- Description: This dissertation will explore the revolutionary progression in the provision of monetary damages and the availability thereof due to the change in South Africa’s legal system from Parliamentary sovereignty to Constitutional supremacy after the enactment of the final Constitution in 19961. The Constitution of South Africa brought with it the concepts of justification and accountability as the Bill of Rights enshrines fundamental rights and the remedies for the infringement of same. The available remedies for the infringement of a fundamental right flow from two sources, being either from the development of the common law remedies in line with the Bill of Rights or alternatively from Section 38 of the Constitution, which provides for a remedy which provides ‘appropriate’ relief. The question that will be raised in this dissertation is, ‘does appropriate relief include an award of delictual damages?’ or a question related thereto ‘is an award of monetary damages an appropriate remedy?’ The motivation for this dissertation arises from the plethora of case law, especially in the Eastern Cape, that has come to the fore in the last sixteen years, highlighting the injustice of cancellations of social assistance grants and the non-payment of such in South Africa’s social security system, as well as the precedent that was set by our Constitutional Court and Supreme Court in remedying that injustice. The central case to this dissertation is that of Kate v Member of Executive Council for Department of Welfare, Eastern Cape 2005 1 SA 141 SECLD; Member of Executive Council, Department of Welfare, Eastern Cape v Kate 2006 (4) SA 478 (SCA), which is generally regarded as having paved the way for the granting of monetary damages for the infringement of an individual’s constitutional right as same require legal protection. Firstly the past approach to damages will be explored in relation to South Africa’s common law, being the Roman-Dutch law. The common law Aquilian action is the focal point of this dissertation in relation to the common law in that the granting of damages for the infringement of an individual’s social assistance right (being a specific constitutional right framed within the 1996 Constitution) results in pure patrimonial loss which in our common law system was remedied by the actio legis Aquilae. In delict, an award of damages is the primary remedy, aimed at affording compensation in respect of the legal right or interest infringed. After the common law system of damages has been explored, this dissertation will then examine the changes that have developed therefrom, and largely shaped by the current state of disorganization in the National Department of Welfare coupled with the all encompassing power of the final Constitution. The final Constitution provides the power, in section 38 of the 1996 Constitution, for the court to award a monetary remedy for the breach of a constitutional right. The question, however, is “does the award of monetary damages not merely throw money at the problem, whereas the purpose of a constitutional remedy is to vindicate guaranteed rights and prevent or deter future violations?” The battle for domination between the common law approach and the constitutional approach to damages is witnessed as the two systems eventually amalgamate to form an essentially new remedy, unique to South Africa. South Africa’s new system is aligned with the Constitution as the Constitution is the supreme law of the land and underpins the awarding of all damages and, especially, the awarding of constitutional damages. For the sake of completeness, alternatives to monetary damages will also be canvassed in this dissertation. It is hoped that the reader will, in the end, realize that the final Constitution is the supreme law of the land and as such dictates the manner and form in which damages are provided. If such provision is not in alignment with the Constitution, it will be declared invalid. The flexibility of our common law is put to the test, yet it is found to be adaptable to the ever-developing boni mores of society exemplified in the embracing constitutional principles and the production of this new remedy. The courts develop the common law under section 39(2) of the Constitution in order to keep the common law in step with the evolution of our society and the ever changing nature of bonos mores. , National Research Foundation
- Full Text:
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