Molecular study of mycobacterium tuberculosis complex (MTBC) DNA from Port Elizabeth
- Authors: Londiwe, Bhembe Nolwazi
- Date: 2014
- Subjects: Mycobacterium tuberculosis
- Language: English
- Type: Thesis , Masters , MSc (Microbiology)
- Identifier: vital:11281 , http://hdl.handle.net/10353/d1016163 , Mycobacterium tuberculosis
- Description: Mycobacterium tuberculosis complex (MTBC) is a causative agent of tuberculosis (TB) in humans and animals. The burden of tuberculosis in South Africa is worsened by the concurrent epidemic of HIV. The dynamic of TB epidemics has been investigated and yet little data has been given about the Eastern Cape, particularly Port Elizabeth. The study aimed to investigate the prevalence of drug resistant MTBC and to determine the mutations causing resistance in Port Elizabeth. One hundred and ninety (190) DNA samples isolated from sputum specimen in humans suspected of having TB were amplified using the Seeplex® MTB Nested ACE detection assay. To differentiate Mycobacterium tuberculosis complex (MTBC) members for surveillance purposes a multiplex polymerase chain reaction (PCR) method was done based on genomic regions of differences such as RD1, RD1mic, RD2seal, RD4, RD9 and RD12. Target genes known to confer resistance to first and second-line drugs were amplified and the amplicons sequenced using Big Dye Terminator DNA sequencing kit v3.1 (Applied Biosystems, UK). The patient’s demographic profiles were obtained from the National Health Laboratory Service (NHLS). All hundred and ninety DNA samples tested positive for MTBC using the Seeplex® MTB Nested ACE assay. Results show a high prevalence of extensive drug resistant TB in Port Elizabeth, Eastern Cape Province. One hundred and eighty four (184) DNA isolates were used in the identification of different MTBC species. We ended up working with 184 DNA isolates because we ran out of DNA, and we could not go back to isolate DNA from the affected individuals due to the fact that some patients died, while some have been released to go to their homes. From the 184 DNA isolates 45 (24.5%) isolates were identified to be M. tuberculosis, 94 isolates (51.1%) to be M. bovis BCG and 3 isolates (1.6%) to be M. cannetti. Sequencing results show the position of mutation in each DNA isolate; however in the study we got resistance to MDR to be 100% and 42% pre-XDR while 58% was XDR. These results raise an alarm for the prevalence MDR in MTBC from Port Elizabeth. This is a serious health concern which calls for a need to strategise on the identification of extensive drug resistant TB patients from multi-drug resistant TB patients and ensure monitoring of their treatment.
- Full Text:
- Date Issued: 2014
- Authors: Londiwe, Bhembe Nolwazi
- Date: 2014
- Subjects: Mycobacterium tuberculosis
- Language: English
- Type: Thesis , Masters , MSc (Microbiology)
- Identifier: vital:11281 , http://hdl.handle.net/10353/d1016163 , Mycobacterium tuberculosis
- Description: Mycobacterium tuberculosis complex (MTBC) is a causative agent of tuberculosis (TB) in humans and animals. The burden of tuberculosis in South Africa is worsened by the concurrent epidemic of HIV. The dynamic of TB epidemics has been investigated and yet little data has been given about the Eastern Cape, particularly Port Elizabeth. The study aimed to investigate the prevalence of drug resistant MTBC and to determine the mutations causing resistance in Port Elizabeth. One hundred and ninety (190) DNA samples isolated from sputum specimen in humans suspected of having TB were amplified using the Seeplex® MTB Nested ACE detection assay. To differentiate Mycobacterium tuberculosis complex (MTBC) members for surveillance purposes a multiplex polymerase chain reaction (PCR) method was done based on genomic regions of differences such as RD1, RD1mic, RD2seal, RD4, RD9 and RD12. Target genes known to confer resistance to first and second-line drugs were amplified and the amplicons sequenced using Big Dye Terminator DNA sequencing kit v3.1 (Applied Biosystems, UK). The patient’s demographic profiles were obtained from the National Health Laboratory Service (NHLS). All hundred and ninety DNA samples tested positive for MTBC using the Seeplex® MTB Nested ACE assay. Results show a high prevalence of extensive drug resistant TB in Port Elizabeth, Eastern Cape Province. One hundred and eighty four (184) DNA isolates were used in the identification of different MTBC species. We ended up working with 184 DNA isolates because we ran out of DNA, and we could not go back to isolate DNA from the affected individuals due to the fact that some patients died, while some have been released to go to their homes. From the 184 DNA isolates 45 (24.5%) isolates were identified to be M. tuberculosis, 94 isolates (51.1%) to be M. bovis BCG and 3 isolates (1.6%) to be M. cannetti. Sequencing results show the position of mutation in each DNA isolate; however in the study we got resistance to MDR to be 100% and 42% pre-XDR while 58% was XDR. These results raise an alarm for the prevalence MDR in MTBC from Port Elizabeth. This is a serious health concern which calls for a need to strategise on the identification of extensive drug resistant TB patients from multi-drug resistant TB patients and ensure monitoring of their treatment.
- Full Text:
- Date Issued: 2014
Sustainable agricultural development in the Malawian smallholder agricultural sector: a case of Lilongwe District
- Authors: Chizimba, Martha
- Date: 2010
- Subjects: Food security -- Malawi , Poverty -- Malawi , Sustainable agriculture -- Malawi Case studies , Sustainable development -- Malawi Case studies , Agriculture -- Malawi
- Language: English
- Type: Thesis , Masters , M Soc Sc (Dev)
- Identifier: vital:11420 , http://hdl.handle.net/10353/365 , Food security -- Malawi , Poverty -- Malawi , Sustainable agriculture -- Malawi Case studies , Sustainable development -- Malawi Case studies , Agriculture -- Malawi
- Description: Even though agriculture is the backbone of Malawi‟s economy, food insecurity has remained a continuous threat among the poor. Until the 1980s, Malawi had been achieving national food security through an extensive system of agricultural inputs and marketing subsidies. However, these subsidies were removed and at the same time, the agricultural credit system collapsed. Consequently, agricultural productivity in Malawi remained low, poverty remained pervasive and food insecurity remains a main constraint to national and household food security. Therefore, the success of the agricultural sector in Malawi is very critical for raising the living standards and for food self-sufficiency. In this vein, the study hypothesized that Malawi can only achieve sustainable agricultural development if its agricultural policies are focused towards intensifying agricultural productivity through active participation of smallholder farmers. The major aim of the study was to contribute towards an improved understanding of how the issues of sustainable agricultural development have been addressed in Malawi and how they have influenced the lives of smallholder farmers. The analysis of the results revealed that even though what was implemented in the 1970s to early 1980s was financially unsustainable, but it provided some solutions to the fundamental challenges of smallholder development in Malawi. However, the liberalisations eroded whatever economic benefits achieved then. Never the less, the re-introduction of the agricultural input subsidies restored back the means of production leading to significant transformation of the country from a net importer to a net food exporter. On the other hand, although the agricultural input subsidy programme is being commended for having helped in achieving food security, the study revealed that the programme requires complementary services of credit, extension, research and market to support it. This will provide an exit strategy, which can enable the producers to sell their produce at higher prices sufficient enough for them to afford agricultural inputs without subsidies.
- Full Text:
- Date Issued: 2010
- Authors: Chizimba, Martha
- Date: 2010
- Subjects: Food security -- Malawi , Poverty -- Malawi , Sustainable agriculture -- Malawi Case studies , Sustainable development -- Malawi Case studies , Agriculture -- Malawi
- Language: English
- Type: Thesis , Masters , M Soc Sc (Dev)
- Identifier: vital:11420 , http://hdl.handle.net/10353/365 , Food security -- Malawi , Poverty -- Malawi , Sustainable agriculture -- Malawi Case studies , Sustainable development -- Malawi Case studies , Agriculture -- Malawi
- Description: Even though agriculture is the backbone of Malawi‟s economy, food insecurity has remained a continuous threat among the poor. Until the 1980s, Malawi had been achieving national food security through an extensive system of agricultural inputs and marketing subsidies. However, these subsidies were removed and at the same time, the agricultural credit system collapsed. Consequently, agricultural productivity in Malawi remained low, poverty remained pervasive and food insecurity remains a main constraint to national and household food security. Therefore, the success of the agricultural sector in Malawi is very critical for raising the living standards and for food self-sufficiency. In this vein, the study hypothesized that Malawi can only achieve sustainable agricultural development if its agricultural policies are focused towards intensifying agricultural productivity through active participation of smallholder farmers. The major aim of the study was to contribute towards an improved understanding of how the issues of sustainable agricultural development have been addressed in Malawi and how they have influenced the lives of smallholder farmers. The analysis of the results revealed that even though what was implemented in the 1970s to early 1980s was financially unsustainable, but it provided some solutions to the fundamental challenges of smallholder development in Malawi. However, the liberalisations eroded whatever economic benefits achieved then. Never the less, the re-introduction of the agricultural input subsidies restored back the means of production leading to significant transformation of the country from a net importer to a net food exporter. On the other hand, although the agricultural input subsidy programme is being commended for having helped in achieving food security, the study revealed that the programme requires complementary services of credit, extension, research and market to support it. This will provide an exit strategy, which can enable the producers to sell their produce at higher prices sufficient enough for them to afford agricultural inputs without subsidies.
- Full Text:
- Date Issued: 2010
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