Assessment of the antibacterial properties of n-Hexane extract of Cocos Nucifera and its interactions with some conventional antibiotics
- Authors: Akinyele, Taiwo Adesola
- Date: 2011
- Subjects: Coconut palm , Microbial sensitivity tests , Gram-negative bacterial infections , Vibrio infections , Antibiotics , Hexane , Extracts
- Language: English
- Type: Thesis , Masters , MSc (Microbiology)
- Identifier: vital:11245 , http://hdl.handle.net/10353/416 , Coconut palm , Microbial sensitivity tests , Gram-negative bacterial infections , Vibrio infections , Antibiotics , Hexane , Extracts
- Description: Cocos nucifera belong to the family Aracaceae (palm Family). The English name is coconut and it is used extensively as medicinal remedies against infections such as urinary tract infections, gastro intestinal infections, skin and wound infections. The in vitro antibacterial (including anti-listerial and anti-vibrio) properties as well as the evaluation of the combination potentials of the plant extract with six front-line antibiotics were evaluated in this study using standard procedures. The in vitro anti-listerial properties of the crude aqueous and n-Hexane extract of the husk of Cocos nucifera were carried out against 37 Listeria isolates. Twenty-nine of the test organisms were susceptible to the aqueous extract while thirty were susceptible to the n-Hexane extract both at the screening concentration of 25 mg/ml. Minimum Inhibitory Concentration (MIC) values for all the susceptible bacteria ranged between 0.6 - 5.0 mg/ml. For the aqueous extract, average log reduction in viable cell count ranged between 0.32 Log10 and 4.8 Log10 CFU/ml after 8 hours interaction in 1 × MIC and 2 × MIC. For the n-Hexane extract, the log reduction ranged between 2.4 Log10 and 6.2 Log10 CFU/ml after 8 hours interaction in 1 × MIC and 2 × MIC. The time-kill characteristics of the two extracts suggest that at higher concentration (2 × MIC) and longer duration of interaction (8 hr), more bacteria were killed. In vitro anti-vibrio and antibacterial properties experiment revealed that of all the 45 vibrio and 25 bacteria strains that was tested, 37 were susceptible to the aqueous extract and 38 to the n-Hexane extract, while 17 were susceptible to the aqueous extract and 21 to the n-Hexane extract. Minimum Inhibitory Concentration (MIC) values for all the susceptible bacteria ranged between 0.3 - 5.0 mg/ml. viii The time kill studies revealed that for the aqueous extract, average log reduction in viable cell count in time kill assay ranged between 0.12 Log10 and 4.2 Log10 CFU/ml after 8 hr interaction at 1 × MIC and 2 × MIC. For the n-Hexane extract, the log reduction ranged between 0.56 Log10 and 6.4 Log10 CFU/ml after 8 hr interaction in 1 × MIC and 2 × MIC. In the test for the combination interactions, the checkerboard method revealed synergy of 67% and indifferent of 33%, while the time kill assay detected synergy in 72% and indifferent in 28% of the combinations tested. The synergy detected was not specific to any of the antibiotics or the Gram reaction of the bacteria, and no antagonism was detected. We conclude that the aqueous and n-Hexane extract of the husk of C. nucifera contains potential broad spectrum antibiotics resistance modulating compounds that could be relevant in the treatment of infections caused by these pathogens. In addition, the husk which is being discarded as agro waste will opens up a vista of opportunities for utilization for therapeutic purposes
- Full Text:
- Date Issued: 2011
- Authors: Akinyele, Taiwo Adesola
- Date: 2011
- Subjects: Coconut palm , Microbial sensitivity tests , Gram-negative bacterial infections , Vibrio infections , Antibiotics , Hexane , Extracts
- Language: English
- Type: Thesis , Masters , MSc (Microbiology)
- Identifier: vital:11245 , http://hdl.handle.net/10353/416 , Coconut palm , Microbial sensitivity tests , Gram-negative bacterial infections , Vibrio infections , Antibiotics , Hexane , Extracts
- Description: Cocos nucifera belong to the family Aracaceae (palm Family). The English name is coconut and it is used extensively as medicinal remedies against infections such as urinary tract infections, gastro intestinal infections, skin and wound infections. The in vitro antibacterial (including anti-listerial and anti-vibrio) properties as well as the evaluation of the combination potentials of the plant extract with six front-line antibiotics were evaluated in this study using standard procedures. The in vitro anti-listerial properties of the crude aqueous and n-Hexane extract of the husk of Cocos nucifera were carried out against 37 Listeria isolates. Twenty-nine of the test organisms were susceptible to the aqueous extract while thirty were susceptible to the n-Hexane extract both at the screening concentration of 25 mg/ml. Minimum Inhibitory Concentration (MIC) values for all the susceptible bacteria ranged between 0.6 - 5.0 mg/ml. For the aqueous extract, average log reduction in viable cell count ranged between 0.32 Log10 and 4.8 Log10 CFU/ml after 8 hours interaction in 1 × MIC and 2 × MIC. For the n-Hexane extract, the log reduction ranged between 2.4 Log10 and 6.2 Log10 CFU/ml after 8 hours interaction in 1 × MIC and 2 × MIC. The time-kill characteristics of the two extracts suggest that at higher concentration (2 × MIC) and longer duration of interaction (8 hr), more bacteria were killed. In vitro anti-vibrio and antibacterial properties experiment revealed that of all the 45 vibrio and 25 bacteria strains that was tested, 37 were susceptible to the aqueous extract and 38 to the n-Hexane extract, while 17 were susceptible to the aqueous extract and 21 to the n-Hexane extract. Minimum Inhibitory Concentration (MIC) values for all the susceptible bacteria ranged between 0.3 - 5.0 mg/ml. viii The time kill studies revealed that for the aqueous extract, average log reduction in viable cell count in time kill assay ranged between 0.12 Log10 and 4.2 Log10 CFU/ml after 8 hr interaction at 1 × MIC and 2 × MIC. For the n-Hexane extract, the log reduction ranged between 0.56 Log10 and 6.4 Log10 CFU/ml after 8 hr interaction in 1 × MIC and 2 × MIC. In the test for the combination interactions, the checkerboard method revealed synergy of 67% and indifferent of 33%, while the time kill assay detected synergy in 72% and indifferent in 28% of the combinations tested. The synergy detected was not specific to any of the antibiotics or the Gram reaction of the bacteria, and no antagonism was detected. We conclude that the aqueous and n-Hexane extract of the husk of C. nucifera contains potential broad spectrum antibiotics resistance modulating compounds that could be relevant in the treatment of infections caused by these pathogens. In addition, the husk which is being discarded as agro waste will opens up a vista of opportunities for utilization for therapeutic purposes
- Full Text:
- Date Issued: 2011
An exploration of family group conferencing as part of the diversion experience of young male offenders
- Authors: Tshem, Tembakazi
- Date: 2009
- Subjects: Juvenile delinquents -- South Africa -- Eastern Cape , Social group work , Social work with criminals -- South Africa -- Eastern Cape , Probation -- South Africa -- Eastern Cape , Family social work
- Language: English
- Type: Thesis , Masters , M SW
- Identifier: vital:11756 , http://hdl.handle.net/10353/210 , Juvenile delinquents -- South Africa -- Eastern Cape , Social group work , Social work with criminals -- South Africa -- Eastern Cape , Probation -- South Africa -- Eastern Cape , Family social work
- Description: The aim of this study is to explore the experiences and evaluations of young male offenders who were diverted to family group conferencing in order to improve current services. An exploratory research design was chosen to obtain an understanding of what these young male offenders experienced during their conferencing and also to seek out their recommendations for future improvement of the family group conferencing diversion programme. This study comprised two samples which were purposively selected. The first sample was made up of ten young male offenders and the second sample comprised of the parents and /or guardians of the young male offenders. The data was collected using structured interview schedules. The data was collected on separate occasions for the two samples. Each interview lasted about 30- 40 minutes each. The findings of this study showed that there is still a need to train probation officers on appropriate implementation of family group conferencing and that families also have a role to play towards succefull implementation of diversion programmes. It is evident from the findings that young offenders need their families to support and care for them during difficult situations, as purported by the systems theory whereby society is regarded as a system with different parts and families are an important part of this system. Young offenders belong to families and families are part of communities and society. It is also important to note that victims are part of families, thus also part of society and the system. Family group conferencing provide an opportunity for all these parts to meet in a mediated environment to address the harm caused and make amends. The findings of this study also showed that there is a need to educate the general public about child justice legislation and the emerging trends in the field of child justice including diversion programmes.
- Full Text:
- Date Issued: 2009
- Authors: Tshem, Tembakazi
- Date: 2009
- Subjects: Juvenile delinquents -- South Africa -- Eastern Cape , Social group work , Social work with criminals -- South Africa -- Eastern Cape , Probation -- South Africa -- Eastern Cape , Family social work
- Language: English
- Type: Thesis , Masters , M SW
- Identifier: vital:11756 , http://hdl.handle.net/10353/210 , Juvenile delinquents -- South Africa -- Eastern Cape , Social group work , Social work with criminals -- South Africa -- Eastern Cape , Probation -- South Africa -- Eastern Cape , Family social work
- Description: The aim of this study is to explore the experiences and evaluations of young male offenders who were diverted to family group conferencing in order to improve current services. An exploratory research design was chosen to obtain an understanding of what these young male offenders experienced during their conferencing and also to seek out their recommendations for future improvement of the family group conferencing diversion programme. This study comprised two samples which were purposively selected. The first sample was made up of ten young male offenders and the second sample comprised of the parents and /or guardians of the young male offenders. The data was collected using structured interview schedules. The data was collected on separate occasions for the two samples. Each interview lasted about 30- 40 minutes each. The findings of this study showed that there is still a need to train probation officers on appropriate implementation of family group conferencing and that families also have a role to play towards succefull implementation of diversion programmes. It is evident from the findings that young offenders need their families to support and care for them during difficult situations, as purported by the systems theory whereby society is regarded as a system with different parts and families are an important part of this system. Young offenders belong to families and families are part of communities and society. It is also important to note that victims are part of families, thus also part of society and the system. Family group conferencing provide an opportunity for all these parts to meet in a mediated environment to address the harm caused and make amends. The findings of this study also showed that there is a need to educate the general public about child justice legislation and the emerging trends in the field of child justice including diversion programmes.
- Full Text:
- Date Issued: 2009
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