Characterization of Trypanosoma brucei Sti1 and its interactions with Trypanosoma brucei Hsp83 and human Hsp90
- Authors: Jamabo, Miebaka
- Date: 2023-03-31
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/422629 , vital:71963 , DOI 10.21504/10962/422629
- Description: Neglected tropical diseases continue to pose global concern due to their impact on health and socio-economic status of developing countries in sub-Saharan Africa. African trypanosomiasis is one of the neglected tropical diseases caused by the kinetoplastid flagellate parasite Trypanosoma brucei (T. brucei). The disease is fatal if untreated and the toolbox to combat the disease has been plagued with many difficulties such as drug resistance, toxic chemotherapeutics, and cumbersome drug delivery processes. In recent years, the disease has received attention from organizations such as the Drugs for Neglected Diseases initiative (DNDi) in partnership with WHO as well as academia and industry to provide alternatives to the existing drugs as part of a targeted approach to eliminate human African trypanosomiasis by 2030. The life cycle of the T. brucei parasite requires that it transitions between a cold-blooded vector (the tsetse fly) and a human host. To survive this extreme environmental change and maintain its infectious cycle, the parasite has evolved an arsenal of tools which include a strong immune evasion technique and a robust molecular chaperone system. Heat shock protein 90 (Hsp90) is one of the most abundant eukaryotic molecular chaperones that has been extensively studied in many organisms. It is indispensable for maintaining proteostasis in some organisms and its inhibition is currently being explored as a drug target for cancer and other parasitic diseases. In T. brucei, cytosolic Hsp90 is specifically referred to as Hsp83 due to variations in the sizes amongst different orthologues. Hsp90 is present in high levels in all stages of the T. brucei cell cycle both constitutively and on exposure to stress. To function in the cell, Hsp90 is dependent on co-chaperones, one of which can be found in most organisms, namely, the stress-inducible protein 1 (Sti1). The Hsp90-Sti1 interaction was shown to be crucial for growth in the intracellular kinetoplastid parasite, Leishmania donovani. However, this partnership has not been explored in the extracellular parasite T. brucei. To analyse the interaction of Hsp90 with Sti1 in T. brucei, this study combined in silico, in vitro and in vivo tools. In silico analyses of the Hsp90 complement in T. brucei revealed the presence of twelve putative Hsp90 genes, ten of which code for the cytosolic protein and are arranged in tandem in a head to tail fashion on the same chromosome. One gene each was found for the mitochondrial and ER paralogues of Hsp90, similar to all other species analysed. Eight putative co-chaperones specific to T. brucei were also discovered: six tetratricopeptide repeat domain (TPR) containing co-chaperones and two non-TPR containing co-chaperones. Structural and evolutionary analysis also confirmed that the domains were conserved across the species analysed. T. brucei Sti1 (TbSti1), T. brucei cytosolic Hsp90 (TbHsp83) and human cytosolic Hsp90 (hHsp90) were heterologously overproduced in E. coli and purified using nickel affinity chromatography. With specific antibodies, the expression and localization of the proteins were confirmed. TbSti1 showed strong affinity to the Hsp90s in the nanomolar range, with higher affinity for hHsp90 compared to TbHsp83. TbHsp83 and hHsp90 showed typical chaperone properties by suppressing the aggregation of thermolabile substrate MDH at equimolar concentrations and both chaperones had potent ATP hydrolysis activity. TbSti1, on the other hand, showed no MDH suppression activity and did not affect the ATP hydrolysis activity of TbHsp83 or hHsp90. Ex-vivo experiments using HeLa CRISPR Hop knockout (KO) human cell lines transfected with pcDNA3.1(+)HA-TbSti1 revealed TbSti1 also localized to the cytoplasm. The transfected cells showed a distinct fibroblast-like morphology which was different from the circular morphology seen in the Hop KO untransfected and wild type untransfected cells. Finally, co-immunoprecipitation studies revealed that TbSti1 co-immunoprecipitated with hHsp90. These results show the first characterization of the TbHsp83-TbSti1 partnership in T. brucei. The strong association between both proteins suggests a functional role for this partnership in T. brucei and could provide an updated context for understanding Trypanosome brucei biology. , Thesis (PhD) -- Faculty of Science, Biotechnology and Innovation Centre, 2023
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- Date Issued: 2023-03-31
Economically important cephalopods of southern Angola, with a focus on the artisanal jig fishery
- Authors: Vázquez Morán, Chénelle Lesley
- Date: 2023-03-31
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/422529 , vital:71955 , DOI 10.21504/10962/422529
- Description: The global decline in marine finfish stocks has been met with a concomitant expansion of fisheries for cephalopods. Despite the increase in the exploitation and commercialization of cephalopods, there is a dearth of scientific information for over 90% of the extant species and this is even more marked in the developing world. Angolan capture fisheries production noted a tenfold increase in cephalopod landings between 1994 and 2003. One of the important fisheries is a cephalopod directed jig fishery that targets the squid Loligo reynaudii and the cuttlefish Sepia vermiculata in southern Angola, however, there is a complete lack of socio-ecological information available for this fishery. Hence the aim of this thesis was to provide basic biological and demographic information on L. reynaudii and S. vermiculata distributed off the coast of southern Angola, assess the importance and dependence of local artisanal fishers on these two species and examine the current management strategies and policies in the region This study found that the artisanal cephalopod jig fishery is critical for the livelihoods of the coastal communities of southern Angola, particularly due to the limited alternative income generating opportunities available. The cephalopod jig fishery is highly labour intensive, with fishers spending a considerable amount of time per day, drifting over known fishing grounds. Catch composition of the artisanal jig fishery indicated that squid is the dominant species within the fishery, in terms of numerical abundance and weight. The productivity and progression of the fishery is hampered by a number of ecological, socio-economic and governance constraints, as highlighted by socio-economic surveys conducted with local squid fishers. Such constraints include, but are not limited to declining catches, increased negative interactions with seals, increased inter-sector competition, lack of infrastructure, poor quality fishing equipment as well as a lack of communication and support from government officials. To examine aspects of the biology and demography of L. reynaudii, whole specimens were collected monthly, from the two primary fishing areas in southern Angola; Namibe and Tombua, during two main sampling periods, June 2008 – May 2009 (Period 1) and June 2014 – May 2015 (Period 2). Male L. reynaudii were found to be significantly larger in size and weight than females, however both the average and maximum dorsal mantle length (DML) decreased between sampling periods. Length-at-50% maturity was larger for male L. reynaudii (170.47 mm DML – 1st period, 165.44 mm DML – 2nd period) than female L. reynaudii (114.71 mm DML – 1st period, 109.43 mm DML – 2nd period). Gonadosomatic indices (GSI) varied significantly during month and season for both sexes. Peak GSI coincided with a low catches in the artisanal jig fishery suggesting that the population may undergo a spawning migration away from fishing grounds, during late spring and summer. The theory of a seasonal spawning migration is further supported in this study by the low abundance of juvenile and sub-adult L, reynaudii collected during the study, along with the inability to detect egg beds in known fishing grounds. Stomach content analysis indicated that teleosts were the most important dietary item (66.3% F of the stomach contents). Trachurus capensis and Engraulis encrasicolus are both highly abundant small pelagic fishes in the cold nutrient rich waters of the Benguela Current and dominated the diet of sampled squid during autumn and winter months. It is likely that the prevalence of large, adult squid off the coast of Angola, as observed within the current study, may be ascribed to the suitable environmental conditions and the abundance of suitable prey. The results from the current study indicate that the southern Angolan subpopulation reveals similarities to that of the West Coast subpopulation of squid in South Africa, with regards to demographics and reproductive characteristics. It is likely that the population in southern Angola is an extension of the South African subpopulation of L. reynaudii on the western extremities. To examine the basic population biology and ecology of S. vermiculata off southern Angola whole specimens were collected from the fishery between December 2014 and April 2015. Morphological results indicate clear sexual dimorphism in soft part characteristics, cuttlebone and beak morphometry. Length-at-50% maturity was smaller in male S. vermiculata (217.53 mm DML) than female S. vermiculata (231.13 mm DML), although these differences were not significant. Stomach content analysis indicated that S. vermiculata predominantly fed on teleost species which were found within 64.5% of stomachs. Teleosts were more dominant in the diet of male S. vermiculata (78.6% F) than female S. vermiculata (53.0% F), which ate a more varied diet including crustaceans (41.2% F) and cephalopods (17.7% F). Availability and abundance of cuttlefish in Namibe indicates that the species may undergo a spatio-temporal shift in distribution, with majority of the artisanal catches of S. vermiculata occurring during summer months. The results from the current study highlights the fisheries potential of L. reynaudii and S. vermiculata and the dependence of artisanal fishers in southern Angola on cephalopods, and more specifically squid. The development of sustainable fisheries management strategies for the artisanal cephalopod jig fishery is critical, particularly as the global exploitation of cephalopods continues to increase with declining finfish populations. Failure to implement management strategies may remove this critical economic safety net for vulnerable coastal communities which have little alternative livelihood opportunities. Ongoing ecological research, a co-management approach and an increased consideration of local ecological knowledge will assist in designing appropriate management plans for the southern Angolan cephalopod jig fishery. , Thesis (PhD) -- Faculty of Science, Ichthyology and Fisheries Science, 2023
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- Date Issued: 2023-03-31
Innovation in ecological restoration techniques: Enhancing Portulacaria afra survivorship in degraded arid thicket
- Authors: Norman, Yondela Masande
- Date: 2023-03-31
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/419276 , vital:71630
- Description: The Albany Thicket Biome has undergone extensive degradation over the past century, particularly from overstocking of livestock in the arid types of thicket. The degradation of the biome, coupled with little to no natural recovery, prompted the South African Government to implement the Subtropical Thicket Restoration Programme (STRP) in the early 2000s to rehabilitate degraded thicket using unrooted Portulacaria afra truncheons. The STRP also sought to create job opportunities, bring about social upliftment, promote biodiversity conservation and incentivise farmers and landowners in the region to promote carbon trading and farming of P. afra using carbon credits. However, the survivorship of P. afra planted using the STRP planting protocol has been less than ⁓30% because they are planted in degraded areas, where the truncheons are subjected to harsh biophysical conditions such as hard, capped soil, high soil temperatures, drought and herbivory. The aim of this study, therefore, was to improve the survivorship of P. afra planted in degraded thicket to at least 30%, using modified planting methods. In this study, four treatments were applied in 210 pondings (or micro-dams), each with P. afra planted in them (the first three treatments used rooted cuttings). The first treatment involved watering regimes where pondings were watered at varying frequencies. The second treatment involved planting companion species alongside P. afra while the third treatment involved planting P. afra underneath a nurse canopy. In the fourth treatment, unrooted P. afra truncheons were planted inside pondings. The mean survivorship of the P. afra cuttings was assessed 18 months after planting. The watering regime treatments, including the control, all yielded a survivorship of over 75%, with the significantly highest levels of survivorship being displayed in the weekly watering treatment (90.9 ± 6.8%) (p < 0.01). The companion plant treatment also produced a high mean survivorship of P. afra (94.9 ± 3.6%), significantly higher than that of the nurse plants (87.5 ± 6.3%) (p < 0.01). Among the unrooted truncheons it was found that untreated truncheons had the highest survivorship (76.2 ± 17.6%), with the lowest mean being found in truncheons that were both pruned and scarified (70.8 ± 20.8%), suggesting that this treatment, out of all of them, is the least successful under the prevailing environmental conditions in the study area. However, there was no significant differences among the unrooted truncheon treatments and their respective survivorship values to further substantiate this assertion (p = 0.26). Findings in this study also suggest that planting P. afra cuttings under a nurse plant was ideal for P. afra survivorship due to the nurse plant’s ability to ameliorate the microclimate under which the cuttings can establish and grow. Despite the soil under the nurse canopy having a significantly lower mean soil water potential (-160.9 ± 200.5 kPa), compared to the open areas (-73.4 ± 55.7 kPa) (p = 0.04), the pondings under the nurse canopy still had a lower mean soil temperature (31.4 ± 5.25°C) than the treatments in the open areas (38.5 ± 2.7°C), during the harsh midday sun. These favourable characteristics are reflected in the P. afra cuttings under a nurse canopy having a higher chlorophyll fluorescence (0.76 ± 0.06). compared to those planted in open areas (0.73 ± 0.13), suggesting that the P. afra cuttings in the former treatment had a higher photosynthetic efficiency compared to those in the latter, despite there being no statistically significant difference in chlorophyll fluorescence between the treatments (p = 0.14). Although these findings suggest that applying these modifications to planting P. afra, having achieved its objective of increasing survivorship to well over 50%, is likely to produce favourable results not only in overall survivorship, but also in fast-tracking arid thicket rehabilitation and restoration, further research on these rehabilitation techniques and their effectiveness is required. Furthermore, the downside to these treatments is that they are costly and time consuming, which puts the feasibility of large-scale programmes using these applications into question. Further investigation is required to determine ways in which the cost-effectiveness of these applications can be enhanced. , Thesis (MSc) -- Faculty of Science, Environmental Science, 2023
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- Date Issued: 2023-03-31
Phytoplankton communities provide insight into ecosystem functioning of the Agulhas Current system
- Authors: Gibb, Ross-Lynne Alida
- Date: 2023-03-31
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/422646 , vital:71965
- Description: Access restricted. Embargoed until 2025. , Thesis (PhD) -- Faculty of Science, Biochemistry and Microbiology, 2023
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- Date Issued: 2023-03-31
Rapid Synthesis of Thiol-Co-Capped CdTe/CdSe/ZnSe Multi-Core-Shell QDs and Their Encapsulation in Liposomes and Chitosan Nanoparticles; Comparative Bio-compatibility Studies Using Hela and Vero Cells
- Authors: Daramola, Olamide Abiodun
- Date: 2023-03-31
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/422617 , vital:71962 , DOI 10.21504/10962/422617
- Description: The common method that has been used to reduce the toxicity posed to living cells by CdTe Quantum Dots (QDs) is through the synthesis of CdTe multi-core-shells nanoparticles. In this process, the surface of CdTe QDs is usually coated by less toxic ZnS or ZnSe shells. This heterostructure compound does not only reduce the toxicity of CdTe QDs but can also be used in applications such as deep tissue imaging. The heterostructures can be in numerous forms such as CdTe/CdSe/ZnSe or CdTe/CdSe/ZnS or CdTe/CdS/ZnS multi-core-shell QDs. However, the drawbacks attributed to the fabrication of these compounds is long synthesis times (6- 24 h) in achieving the highest wavelength emission maxima. Others are the use of toxic reagents and poor reproducibility of synthesized materials. An additional problem is that the ZnSe or ZnS coating is insufficient to completely protect the highly toxic Cd metal from escaping into immediate solution. This limits their use in biochemistry and with living systems. Liposomes and biopolymers such as chitosan are known to be environmentally friendly compounds that have been used in various studies as delivery systems for QDs and model drugs for drug delivery applications. They are generally non-toxic and highly bio-compatible. In this study, the rapid synthesis of thiol-co-capped CdTe/CdSe/ZnSe multi-core-shell QDs with a maximum reaction time of 35 mins, gave reliable QDs with emission maxima at 625 nm. The multi-core-shell QDs were encapsulated in two different bio-compatible environments, namely liposome and chitosan nanoparticles (CNP) at 14 different formulations (F) for liposome and 12 different formulations for CNP. Cytotoxicity and florescence imaging studies using HeLa and Vero cells, were used to investigate the improved bio-compatibility. Various characterization techniques were used to elucidate the optical properties, morphology and physico-chemical properties of the QDs and nanocomposites. Two of the best formulations, QD-liposome vesicles (LVs)-F12 and QD-CNP-F9 (with chitosan), demonstrated high loading efficiencies of 42 ± 6 % and 59 ± 5 %, respectively. While the plain CdTe QDs showed high toxicity, some of the encapsulated materials, QD-LVs-F1 and F12, depicted no-toxicity against the cells (IC50 > 0.5 mg/ml). The QDs also retained most of their fluorescence and properties and could easily be tracked in cells and visualized around the nucleus, indicating the successful internalization of the QDs in the cytosol. These results shows that encapsulation of CdTe multi-core-shell QDs in liposomes produce better bio-compatibility compared to multi-core-shell QDs and better than CNP coating. These particles therefore show good promise in cell-labelling, drug delivery studies. Their core-shell nanoparticles have also shown good behavior in enhancing the memory of a device which is based on some recent collaborated works. , Thesis (PhD) -- Faculty of Science, Chemistry, 2023
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- Date Issued: 2023-03-31
South African supratidal microbialites: prokaryote communities, metabolic capabilities, and biogeochemical processes
- Authors: Isemonger, Eric William
- Date: 2023-03-31
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/422641 , vital:71964
- Description: Access restricted. Embargoed until 2025. , Thesis (PhD) -- Faculty of Science, Biochemistry and Microbiology, 2023
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- Date Issued: 2023-03-31
The development of ionic zinc(II) phthalocyanines for sono-photodynamic combination therapy of cervical and breast cancer
- Authors: Nene, Lindokuhle Cindy
- Date: 2023-03-31
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/422565 , vital:71958 , DOI 10.21504/10962/422565
- Description: This study focuses on the development of the sono-photodynamic combination therapy (SPDT) activity of phthalocyanines (Pcs) on the cervical and breast cancer cell lines in vitro. The SPDT technique utilizes ultrasound in combination with light to elicit cytotoxic effects for cancer eradication. In this work, a selection of tetra-peripherally substituted Zn(II) cationic and zwitterionic Pcs were prepared. The photophysical parameters of the Pcs were determined including their fluorescence behaviours and efficiency of the triplet excited state population. The effects of the ultrasonic parameters (frequencies (MHz) and power (W.cm-2)) on the stability of the Pcs were evaluated. Four parameters were evaluated: Par I (1 MHz: 1 W.cm-2), Par II (1 MHz: 2 W.cm-2), Par III (3 MHz: 1 W.cm-2) and Par IV (3 MHz: 2 W.cm-2). The stability of the Pcs reduced with the increase in the ultrasonic power (for Par II and Par IV). The Par I showed the least degradation compared to the other parameters and was therefore used for the SPDT treatments. The sonodynamic (SDT), photodynamic (PDT) therapy activities of the Pcs were studied and compared to their SPDT efficacies. The Pcs showed reactive oxygen species generation during the SDT, PDT and SPDT treatments. For the SDT and SPDT, singlet oxygen (1O2) and hydroxyl radicals (•OH) were detected. For PDT, only the 1O2 were detected. The cell cytotoxicity studies for the Pcs showed relatively higher therapeutic efficacies for the SDT treatments compared to the PDT treatments, where the SPDT showed higher therapeutic efficacies compared to both the SDT and PDT monotreatments on both the cell lines in vitro. Overall, the combination treatments were better compared to the monotreatments. The activities of the Pcs were compared by their differences in structures, including the type of R-group, type of quaternizing agent and type of nanoparticle conjugates. , Thesis (PhD) -- Faculty of Science, Chemistry, 2023
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- Date Issued: 2023-03-31
A discourse analysis of media representation of women political leaders in Uganda
- Authors: Kemirembe, Grace
- Date: 2023-03-30
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/408908 , vital:70536
- Description: This study is a qualitative desktop research project. The study employed a Feminist Critical Discourse Analysis to analyse and unpack the discursive ways in which female politicians are discussed and talked about in Ugandan online media. This study was a response to the realisation that online media portrayals of female politicians in Uganda remain largely unexplored. Additionally, the study discovered that the Daily Monitor and The Observer, the two newspapers that this thesis researched, often employ gender stereotypes and sexist coverage of female politicians in Uganda using personalisation, trivialisation and demonisation frames. The study illustrates that these misogynistic frames are intended to diminish women’s importance in the political sphere. Moreover, women who do not conform to the gender stereotypes are portrayed as social deviants. This work concludes that one of the challenges faced by women politicians in Uganda, and in Africa as a whole, is how to exploit online media’s productive capacity while, at the same time, resisting its use as an instrument that undermines them. Given the limited scope of the study using only two media organisations, future studies on media representation of female politicians could expand the range to include print and visual sources to provide generalisable results. , Thesis (MA) -- Faculty of Humanities, Political and International Studies, 2023
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- Date Issued: 2023-03-30
An analysis of womxn’s understanding of South African public health awareness campaigns’ messages on HIV/AIDS using cognitive interviewing
- Authors: Simpo, Hazel
- Date: 2023-03-30
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/408738 , vital:70521
- Description: Besides having the most extensive antiretroviral therapy (ART) treatment programme, South Africa faces the highest epidemic rate, with womxn and children most affected. Several prevention strategies have been implemented to curb the spread of HIV/AIDS in South Africa, including free access to print form awareness campaigns that come through pamphlets, posters and billboards. However, most public health sector awareness and educational programmes carry uniform messages that are then carried out in heterogeneous contexts leading to the miscomprehension of the intended messages. This study aimed to explore womxn’s understanding of public health awareness content on the prevention of HIV/AIDS. The focus was on examining their interpretation of universal HIV/AIDS awareness messages in their heterogeneous contexts, which might provide obstacles to womxn’s reproductive health and well-being using a reproductive justice framework. This study employed a cognitive interviewing methodology to collect data from the participants to obtain information about how participants understand and perceive knowledge and how they arrive at specific answers or judgements. Twenty-eight (28) womxn aged between 18 and 30 years were purposively sampled for this study from four (4) local healthcare facilities in Makhanda: the Grahamstown Municipality (Anglo-African) Clinic, the Joza Clinic, the Settlers Day Hospital and the Raglan Road Clinic. Thematic network analysis (Attride-Stirling, 2001) was used to analyse the data collected from the study. One global theme emerged from the dataset: Barriers to HIV prevention, care and treatment. The barriers were identified mainly due to social constructions of hegemonic masculinity, individual versus cultural expectations, and outdated awareness content. The findings from the study suggest that almost all the challenges that womxn faced in terms of understanding and interpreting the awareness materials stemmed from structural factors in the South African context. The findings also suggest that cognitive interviewing methodology can be reconceptualised as an endeavour that cuts across a wide range of fields. , Thesis (MSocSci) -- Faculty of Humanities, Psychology, 2023
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- Date Issued: 2023-03-30
Babantu ababomvu entliziyweni(People who are ‘Red’ in their hearts) and the myth of ‘modernity’
- Authors: Haynes, Duncan Robert
- Date: 2023-03-30
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/408897 , vital:70535
- Description: This study examines a history of urban research on ancestral belief of amaXhosa within the Greater East London region, Eastern Cape, South Africa which spanned much of the 20th century (1934–2002). Through extensive textual and literary investigation, this thesis offers new perspectives on this topic. Current theoretical explorations offer the tools to reassess the positionality of the researchers and in light of findings of these previous studies, highlighting above all, the presence of certain academic ‘blind-spots’. These ‘blind-spots’ are argued to have been caused by theoretical and methodological constraints, particularly around Western-centric definitions of the nature of 'modernity' that rely on the positivist notion of a singular unilinear path of time as a universal standard (and which thus defines 'progressiveness' and 'backwardness'). (These ‘blind spots’ were notably further strengthened by a Eurocentric interpretation of identity and a trend of Neo-Marxist scholarship between the 1970s and the 2010s1 which created a ‘taboo’ on addressing issues of culture, and especially of ancestral spirituality, as this was taken to be portraying Africans as culturally ‘static’ and backward and therefore supporting Apartheid ideologies of ‘separate development’). Through these textual investigations this thesis brings to light the, largely unrecognised, core of the issue or driving factor behind the difficulties in all of these previous studies. This is posited as the existence of a prevailing culture of ‘silence’ in Xhosa spaces (stretching from 1840–2000) regarding the sustained importance of 'Redness' (ancestral spirituality and traditional dimensions of identity due to the notion that it was 'backward'). This self-censoring is unpacked as having been an expedient response by many to the requirements for Xhosa pagans to wash off the symbolic red cosmetic clay at baptism, put on ‘decent’ Western clothing and shoes and take on Christian first names (‘shedding’ their previous names and identities) as processes deemed necessary to properly convert to Christianity (Frescura, 2015). More significantly however, it has been argued by scholars that adopting Christianity was the only way to be able to harness the power of education at a time of starvation related to the famine of the millennial Cattle Killing movement (1856–7) and the Xhosa military defeat at the end of the 100-year period of the British Xhosa Frontier Wars (1779–1879). This thesis uses perspectives offered by Queer theory to unpack the pain of this 'veiling' of aspects of identity as a necessary strategy for achieving economic 'success' amid the structural and epistemological violence of colonialism and apartheid. It is suggested that a combination of the ‘silence’ and ‘taboo’ area of the ‘blind-spot’ could completely ‘veil’ these epistemological realities from the view of many earlier researchers of urban Xhosa identity (whose cultural and linguistic backgrounds as well as etic positioning caused them to be unable to 'knock' to gain access into this normally ‘veiled’ world). This thesis combines these historical perspectives with contemporary scholarship and literary works to propose that these ‘Red’ aspects of identity and spirituality have always and still ‘continue’ to form a core, or crux, of Xhosa identity for a previously widely unrecognised and overwhelming majority of urban amaXhosa. This thesis uses the perspective offered by a Xhosa translation of the title of Mda's English language novel, 'A Heart of Redness’ (2000), to explore the importance of ancestral spirituality and clan identities to amaXhosa as a [previously invisible/veiled] ‘Redness of one's Heart’ for a high proportion of contemporary amaXhosa. In this way, this thesis agrees with scholars such as Latour (1993) and Mignolo (2007) in their 'jarring' expose regarding the epistemic violence of euro-normative notions of ‘modernity’. Here they call for a disruption of the pervasive idea of the West as being at the 'forefront' of human experience and even a challenge to the western reading of time. Finally, through exploring critique of the 'Secularisation Thesis' of the Western world, this thesis poses the implicit question contained in its title: ‘Is the western world now perhaps able to embrace a 'modernity' and integrative-cosmology that has been safe-guarded by Xhosa pagans since the 1840s?’ This study documented numerical indicators on the presence of ancestral belief and traditional dimensions of Xhosa identity from fieldwork conducted in an urban locality King William’s Town (Qonce) within the Buffalo City Municipality, Eastern Cape of South Africa from September 2018 to December 2019. In-depth quantitative and qualitative data were collected from lengthy interactions with 305 consenting Xhosa participants. These interactions comprised 129 semi-structured and 176 structured interviews, 61 of the latter formed the sample for numerical data analysis with which to give clarity on the abiding importance of ancestral spirituality for a vast majority of urban amaXhosa. , Thesis (MA) -- Faculty of Humanities, Anthropology, 2023
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- Date Issued: 2023-03-30
Constructions of Ukuyalwa and marriage satisfaction: experiences of Xhosa couples in the Eastern Cape
- Authors: Bikwe, Siphuxolo
- Date: 2023-03-30
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/408805 , vital:70527
- Description: Marital satisfaction may be described as the extent to which one’s expectations, desires and needs are being satisfied in their marriage. Studies that concern marital satisfaction provide us with crucial insights into factors that support or compromise the longevity of long-term, committed relationships. Little work has been done in understanding the role of cultural practices in marital satisfaction particularly in Africa, and South Africa more specifically. Ukuyalwa is a Xhosa ritual that takes place during a Xhosa traditional wedding, whereby the bride is introduced to the (traditional) guiding practices of her new home by the women of the family together with her sisters-in-law. This study informs the practice of African psychology as formulated by Ratele (2017b).Critical African psychology as a framework recognizes the construction of culture alongside political, socioeconomic, and historical matrices. These are acknowledged as an important element in psychological theories, methods, and explanations. This research project aimed to identify the discourses that circulate around ukuyalwa as a Xhosa cultural practice and consider the implications for the marital satisfaction of amaXhosa couples. The study interviewed 3 Xhosa couples of varying ages who had been married for different durations to develop an understanding of their experiences of ukuyalwa and marital satisfaction. Data was analyzed using a Foucauldian Discourse Analysis (FDA) which allowed for the identifications of the discourses that the couples used to construct ukuyalwa and marital satisfaction. The couples discursively constructed marriage as ‘divine unity’, ‘unification of families’ and ‘partnership’. Ukuyalwa was constructed as a ‘gendered process’, ideas of tradition and modernity, and a ‘foundation’ also became apparent. Satisfaction was constructed as ‘open communication’ and ‘work’. The couples’ positions shifted in their conversations, where in some instances they were 'learners’, ‘initiates’, and/or ‘passive’, and in other instances, they were ‘in charge’, ‘knowledgeable’, and ‘experts’. The discourses of culture, religion, and partnership circulated prominently in relation to marriage, ukuyalwa, and marital satisfaction. The findings of the study speak to how the couples’ constructions of marriage and cultural customs such as ukuyalwa are valuable as they often inform how couples behave in their marriage relationships and what they regard as satisfactory to them when it comes to marriage. These insights into what informs the couples’ ideas on marriage and satisfaction can be utilised in informing couples therapy interventions. , Thesis (MA) -- Faculty of Humanities, Psychology, 2023
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- Date Issued: 2023-03-30
Enforced sojourn: Zimbabwean dispensation, special and exemption permits
- Authors: Maziyanhanga, Zvikomborero
- Date: 2023-03-30
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/408913 , vital:70537
- Description: This thesis investigates Zimbabwean’s immigration to South Africa. Zimbabwean’s immigration to South Africa dates back to the early 2000s. This thesis uses a combination of theories to interrogate and discuss the Zimbabwe special permits and some of the post-apartheid government’s amendments to the South African Citizenship Act and other immigration policies. Some of the theories that theoretically underpin this research project’s methodology include Marxism, Pan-Africanism, Liberalism and culture-based theories. This thesis interrogates the discursive strategies these permits draw on to frame and understand Zimbabwean immigrants in South Africa. This study has found that these permits use similar operating logic as the White South African governments used the migrant labour system to exploit Blacks from all of Southern Africa in the 20th century. For instance, the migrant labour system used during apartheid made all Blacks in South Africa “guest workers” who could be deported at the government’s whim. The apartheid government used racist pass laws to regulate the movement of Black people in South Africa, whereas the post-apartheid government uses Zimbabwean special permits to regulate the movement of Zimbabweans in South Africa. The pass laws were fundamentally racist, and their ultimate objective was to reinforce the idea of White citizenship, whereas the Zimbabwe special permits are not racist. Their colonial similarity, however, lies in how they make Zimbabwean migrants perpetual migrants in South Africa and the various ways in which they cast Zimbabweans as not deserving of South African citizenship. These special permits force Zimbabwe migrants to become “guest workers” who build the post-apartheid economy and then return home when they are no longer “useful” to the economy. This thesis concludes that the post-apartheid Zimbabwe special permits achieve analogous objectives. , Thesis (MA) -- Faculty of Humanities, Political and International Studies, 2023
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- Date Issued: 2023-03-30
Experiences of men vlogging about substance use disorder recovery on YouTube
- Authors: Chele, Palesa Shené
- Date: 2023-03-30
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/408829 , vital:70529
- Description: The journey of substance use recovery is an inherently dynamic and on-going process. It is advanced through secure social support systems. Furthermore, requiring the explicit practice of self-advancement and self-efficacy. However, in South Africa, despite the continued upsurge of substance abuse, there remains a substantial gap between the high demand for treatment and the limited capacity to provide state-funded treatment. In addition, various psychosocial and socio-economic factors further impede access to treatment or recovery support. Thus, within communities facing SUD treatment barriers the exploration of individual lived experiences may deepen understandings of how men experience the journey of substance use recovery. , Thesis (MA) -- Faculty of Humanities, Psychology, 2023
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- Date Issued: 2023-03-30
Nurses’ experiences of an abortion counselling training course and their understandings of quality abortion services
- Authors: Mogonong, Laurah Carolina
- Date: 2023-03-30
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/408862 , vital:70532
- Description: This research study focuses on nurses’ experiences of participating in an abortion counselling training programme in the Eastern Cape Province (E.C), using a grounded theory and symbolic interactionism framework. The study aims to investigate how participating in a women-centred abortion counselling training course offered through the Critical Studies in Sexualities and Reproduction research unit may modify or improve nurses’ practice of abortion counselling and understanding of quality abortion services. Through purposive sampling, nine research participants working as abortion providers in EC health care facilities were recruited. Research data were produced using discussions around prior abortion counselling experiences, a reflective activity, power point case presentations conducted within the counselling training, and semi-structured interviews conducted two months after the training with five of the providers. A grounded theory analysis was used to generate themes that highlighted different aspects that enabled or hindered access to quality abortion services. In accordance with prior research, it was revealed that nurses undergo abortion training but there is limited content on abortion counselling training. Findings from this study reveal the modifications of practice and understanding of quality abortion services of nurses who participated in the client-centred abortion counselling training course. Themes of change that emerged from the nurses’ experiences were rendering counselling services in a non- judgemental, non-directive way, being acknowledged and recognized for the services they offer as abortion providers, and accepting that abortion is a reproductive legal right that can be accessed whenever a woman in need of the service requests it. The findings of this research also indicate that, despite the controversy and stigma attached to providing abortion services, the participants were motivated to continue offering abortion services because they felt equipped since undergoing abortion counselling training. The training course improved reported abortion counselling practices, but systematic issues such as lack of managerial support, space for individual counselling and procedure equipment continue to undermine services. , Thesis (MA) -- Faculty of Humanities, Psychology, 2023
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- Date Issued: 2023-03-30
Pathi’s sister is still troubling
- Authors: Naidoo, Savani
- Date: 2023-03-30
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/408942 , vital:70539
- Description: My thesis is a collection of micro fiction, flash fiction, fairy tales, vignettes and short stories which explore the tension of being both an insider and an outsider. I have access to different cultures without belonging to any of them: as a child, my family moved from a South African Indian community to a formerly whites-only suburb; as an adult I have lived in South Korea, the United Arab Emirates and Sudan. My prose draws on my life experiences, family legends, neighbourhood gossip, news reports and historical events to question norms and ideas that I may have taken for granted had I been fully inside a single culture. In my thesis I frequently spell words phonetically to mimic how I hear or remember them. I also borrow words from languages I don’t speak. I want the languages I use and mix to corrupt each other, as Raymond Federman put it, in order to better express the voices and contexts of the communities I draw inspiration from. Kuzhali Manickavel’s Things We Found During the Autopsy showed me that culturally rich imagery can be used without interrupting narrative flow with explanations. I am also influenced by the poetic sense of rhythm and melody of Lydia Davis’s minimalist prose, and by Sandra Cisneros’s The House on Mango Street, where each concise short story stands alone but together creates a broad understanding of people and place. Anthologies such as PP/FF, edited by Peter Conners, and My Mother She Killed Me, My Father He Ate Me, edited by Kate Bernheimer, have inspired me to be bold in finding the form that best allows each narrative to be told. , Thesis (MA) -- Faculty of Humanities, School of Languages and Literatures, 2023
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- Date Issued: 2023-03-30
Patterns of inclusion and exclusion among trans women in South Africa: a critical narrative inquiry
- Authors: Shabalala, Siyanda Buyile
- Date: 2023-03-30
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/408874 , vital:70533
- Description: Trans women have for the most part remained grossly marginalised in gender development frameworks that have concerned themselves with resolving the historical disenfranchisement of women in patriarchal societies. Considering this continuing systemic erasure of trans subjectivity, this study has aimed to foreground transness, engaging trans women’s experiences of inclusion and exclusion to better understand the traditionally oppressive institutionality of gender in South Africa from a historically silenced trans standpoint. Semi-structured individual interviews were conducted with five trans women living in South Africa. Data was transcribed and analysed using narrative analysis. Mapping the structures, practices and norms that contribute to the marginalisation of trans women in South Africa, the study found that trans women face economic vulnerability driven by familial rejection along with structural discrimination in a gender-biased labour system. Furthermore, trans women were revealed to contend with institutional erasure and stigmatisation in sex-segregated healthcare structures as well as invisibilisation in cisnormative South African knowledge systems that underrepresent trans identities and their viewpoints of the world. Underlyingly, the societal exclusion of trans women emerged as centrally organised by a structure of genderism that rigidly assumes the binariness and fixedness of gender, principally in ways that restrict trans women's right to self-determine as women. Genderism was observed to collude with factors of race and class in a largely capitalist post-apartheid South African society, working concurrently and jointly to reinforce the social exclusion of trans women. However, often subtle narratives of trans social inclusion located marginalising cisgender power in South African democracy as contested terrain. , Thesis (MA) -- Faculty of Humanities, Psychology, 2023
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- Date Issued: 2023-03-30
Pedagogical practices teachers use to teach reading lessons in the junior primary phase in Kavango West region’
- Authors: Shimafo, Hildegard
- Date: 2023-03-30
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/408762 , vital:70523
- Description: Learning to read for meaning and enjoyment in the Junior Primary phase is an essential goal for every child during their primary schooling. Despite this, reading for meaning and enjoyment in the Namibian context, specifically for primary learners, remains a concern. Current research suggests that there are very few studies that have been done in Namibia that examine the pedagogical practices of Junior Primary teachers to develop learners’ reading competence. In view of that, this study focused on the pedagogical practices of Junior Primary teachers in mediating reading in their classrooms. The study is a qualitative interpretive case study using observation and interviews as tools to generate in-depth data on how teachers teach reading. The study sample comprised of three Junior Primary teachers from Grades 1-3 who participated in this study. The selection criteria were based on historical background and the culture of teaching reading lessons for Grade 1-3 teachers. The study revealed some factors that impede the teaching of reading. Amongst them are a lack of in-service training, language barriers, and the lack of resources. The study found that English used as the LoLT (Language of Learning and Teaching) at the school where the study took place made it difficult for the teachers to convey the correct information to the learners due to a lack of proficiency. The study revealed that this situation limited most of the learners’ reading opportunities. Theory of Practice Architecture (ToPA), in particular by Kemmis and Grootenboer (2008), was used as an explanatory and analytical tool. This research asked the questions: 1.What pedagogical practices do Junior Primary teachers employ to mediate reading? and 2. What factors enable and constrain the pedagogical practices of teachers? As a way forward, this study recommends for a compulsory reading program be instituted at Higher Education Institutions responsible for teacher education. This should empower the teachers to enter a Junior Primary classroom with more confidence to teach the various reading components. This study recommends continuous in-service courses for teachers. The study further recommends that the Ministry of Education, Arts and Culture make provisions for textbooks in all schools for all learners if the learners reading performance is to improve in Namibia. , Thesis (MEd) -- Faculty of Education, Education, 2023
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- Date Issued: 2023-03-30
South African traditional healers’ perceptions and treatment of the symptoms of depression
- Authors: Augustine, Daniella Joan
- Date: 2023-03-30
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/408789 , vital:70525
- Description: While depression is experienced worldwide, the World Health Organization indicated that over 80% of this disease burden is found in low- and middle-income countries, with 16% of depressive disorder cases being found in Africa. Specifically, in South Africa, the lifetime prevalence of major depressive disorder is 9.7%. Research conducted on traditional healing estimates that as high as 80% of black individuals in South Africa choose to consult traditional healers. Although there was initial consensus that the use of traditional healers reflected a lack of access to alternative resources, more recent research has however indicated that traditional healers are consulted even when psychiatric facilities and medication are available. Research has illustrated that many patients that chose to consult traditional healers do so as a result of their shared beliefs, and traditional healers' ability to understand the cultural frameworks underlying illness. The current study investigated how traditional healers in the Eastern Cape perceive and treat phenomena currently understood as the symptoms of depression and how these understandings can be incorporated into collaborations between Western medicine and traditional healing practice. A qualitative research design was used to examine the perspectives and treatment methods of isiXhosa traditional healers in the Eastern Cape. Four healers participated in the study. Key findings indicated that there is no single cause or definition of the phenomenon of depression by healers. Causality is greatly attributed to spiritual causes and ancestors play a key role in guiding the diagnosis and multiple treatment interventions employed by healers to treat the experience of depression. Finally, all traditional healers expressed a willingness to collaborate and work with the Western healthcare system, however, they indicated a strong need for greater recognition as a valid healthcare system. , Thesis (MA) -- Faculty of Humanities, Psychology, 2023
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- Date Issued: 2023-03-30
The effectiveness and feasibility of online prolonged exposure therapy for post-traumatic stress among university students
- Authors: Slabbert, Maryna
- Date: 2023-03-30
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/408885 , vital:70534
- Description: Research has found that 70.6% of university students reported exposure to several traumatic events (Hoffman, 2002). Considering the high prevalence of trauma exposure among South African university students, many are at risk of developing post-traumatic stress disorder (PTSD) (Iribarren, Prolo, Naegos & Chiappelli, 2005). Prolonged Exposure Therapy (PE) is regarded as a first-line trauma therapy for PTSD (Foa, Hembree, Rothbaum, & Rauch, 2019). During the Covid-19 pandemic, public health guidelines for physical distancing reduced access to face-to-face mental health care. In response to this, many health care providers adapted to pandemic constraints by meeting their clients online, through Zoom, for example (Wells et al., 2020). Considering the prevalence of trauma exposure among university students, as well as the increased necessity of teletherapy, the study aimed to investigate the effectiveness and feasibility of online PE for PTSD among university students in a South African setting. The study employed Single-Case Experimental Design (SCED) as basis for its research design. Participants included a sample of Rhodes University students (n = 3) who were recruited through purposive sampling. Prior to the intervention, all three participants met the criteria for PTSD, and comorbid depression. Based on the measures used, two of the three participants did not meet the criteria for PTSD post-intervention. In terms of depression symptoms, one participant did not meet the criteria for depression post-intervention, and another participant showed a decrease in depression symptoms. Regarding the feasibility of online PE, all three participants reported the intervention to be feasible, acceptable, and appropriate. Overall, the study provides preliminary results to support the effectiveness and feasibility of online PE. It also gives insight into the value of intervention research and how it can ameliorate PTSD in a South African setting, as well as other low- and middle-income countries (LMICs). , Thesis (MA) -- Faculty of Humanities, Psychology, 2023
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- Date Issued: 2023-03-30
The use of lesson study to assist junior primary ‘in-service training teachers’ to develop learners’ number sense using number talks
- Authors: Ortman-Gaweseb, Dominika
- Date: 2023-03-30
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/408750 , vital:70522
- Description: Thesis embargoed. To be released in 2025. , Thesis (MEd) -- Faculty of Education, Education, 2023
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- Date Issued: 2023-03-30