Towards a capability maturity model for a cyber range
- Authors: Aschmann, Michael Joseph
- Date: 2020
- Subjects: Computer software -- Development , Computer security
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/163142 , vital:41013
- Description: This work describes research undertaken towards the development of a Capability Maturity Model (CMM) for Cyber Ranges (CRs) focused on cyber security. Global cyber security needs are on the rise, and the need for attribution within the cyber domain is of particular concern. This has prompted major efforts to enhance cyber capabilities within organisations to increase their total cyber resilience posture. These efforts include, but are not limited to, the testing of computational devices, networks, and applications, and cyber skills training focused on prevention, detection and cyber attack response. A cyber range allows for the testing of the computational environment. By developing cyber events within a confined virtual or sand-boxed cyber environment, a cyber range can prepare the next generation of cyber security specialists to handle a variety of potential cyber attacks. Cyber ranges have different purposes, each designed to fulfil a different computational testing and cyber training goal; consequently, cyber ranges can vary greatly in the level of variety, capability, maturity and complexity. As cyber ranges proliferate and become more and more valued as tools for cyber security, a method to classify or rate them becomes essential. Yet while a universal criteria for measuring cyber ranges in terms of their capability maturity levels becomes more critical, there are currently very limited resources for researchers aiming to perform this kind of work. For this reason, this work proposes and describes a CMM, designed to give organisations the ability to benchmark the capability maturity of a given cyber range. This research adopted a synthesised approach to the development of a CMM, grounded in prior research and focused on the production of a conceptual model that provides a useful level of abstraction. In order to achieve this goal, the core capability elements of a cyber range are defined with their relative importance, allowing for the development of a proposed classification cyber range levels. An analysis of data gathered during the course of an expert review, together with other research, further supported the development of the conceptual model. In the context of cyber range capability, classification will include the ability of the cyber range to perform its functions optimally with different core capability elements, focusing on the Measurement of Capability (MoC) with its elements, namely effect, performance and threat ability. Cyber range maturity can evolve over time and can be defined through the Measurement of Maturity (MoM) with its elements, namely people, processes, technology. The combination of these measurements utilising the CMM for a CR determines the capability maturity level of a CR. The primary outcome of this research is the proposed level-based CMM framework for a cyber range, developed using adopted and synthesised CMMs, the analysis of an expert review, and the mapping of the results.
- Full Text:
- Date Issued: 2020
- Authors: Aschmann, Michael Joseph
- Date: 2020
- Subjects: Computer software -- Development , Computer security
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/163142 , vital:41013
- Description: This work describes research undertaken towards the development of a Capability Maturity Model (CMM) for Cyber Ranges (CRs) focused on cyber security. Global cyber security needs are on the rise, and the need for attribution within the cyber domain is of particular concern. This has prompted major efforts to enhance cyber capabilities within organisations to increase their total cyber resilience posture. These efforts include, but are not limited to, the testing of computational devices, networks, and applications, and cyber skills training focused on prevention, detection and cyber attack response. A cyber range allows for the testing of the computational environment. By developing cyber events within a confined virtual or sand-boxed cyber environment, a cyber range can prepare the next generation of cyber security specialists to handle a variety of potential cyber attacks. Cyber ranges have different purposes, each designed to fulfil a different computational testing and cyber training goal; consequently, cyber ranges can vary greatly in the level of variety, capability, maturity and complexity. As cyber ranges proliferate and become more and more valued as tools for cyber security, a method to classify or rate them becomes essential. Yet while a universal criteria for measuring cyber ranges in terms of their capability maturity levels becomes more critical, there are currently very limited resources for researchers aiming to perform this kind of work. For this reason, this work proposes and describes a CMM, designed to give organisations the ability to benchmark the capability maturity of a given cyber range. This research adopted a synthesised approach to the development of a CMM, grounded in prior research and focused on the production of a conceptual model that provides a useful level of abstraction. In order to achieve this goal, the core capability elements of a cyber range are defined with their relative importance, allowing for the development of a proposed classification cyber range levels. An analysis of data gathered during the course of an expert review, together with other research, further supported the development of the conceptual model. In the context of cyber range capability, classification will include the ability of the cyber range to perform its functions optimally with different core capability elements, focusing on the Measurement of Capability (MoC) with its elements, namely effect, performance and threat ability. Cyber range maturity can evolve over time and can be defined through the Measurement of Maturity (MoM) with its elements, namely people, processes, technology. The combination of these measurements utilising the CMM for a CR determines the capability maturity level of a CR. The primary outcome of this research is the proposed level-based CMM framework for a cyber range, developed using adopted and synthesised CMMs, the analysis of an expert review, and the mapping of the results.
- Full Text:
- Date Issued: 2020
Towards a community-based model for Agricultural development in Uganda : a case study of Kumi and Gomba districts
- Authors: Kiggundu, Joseph
- Date: 2020
- Subjects: Agricultural development projects -- Uganda
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10948/48654 , vital:41056
- Description: This study intended to design a community-based model for agricultural development in Uganda, using Kumi and Gomba districts as case studies. The surge in attention towards community-based development is attributed to the growing challenges posed by the traditional approaches to community development, dominated by top-down mechanisms during the planning, implementation, management, monitoring, and evaluation phases of community development programmes. Driven by Robert Chambers and Conway’s theoretical perspectives, this study discusses that a community-based model is capable of filling knowledge gaps, not only in scientific research, but also in the community development process of many developing nations, such as Uganda. In this study particularly, a community-based approach has been identified as the better option in aiding and facilitating the current government efforts to transform the agricultural sector from its predominantly subsistence nature to commercial orientation. It has been acknowledged that the Ugandan government and its development partners do realise the significant role agriculture plays in directly or indirectly impacting the social welfare of Ugandans. The agricultural sector has been identified as a major source of livelihood for most rural Ugandans (over 95% depend on it for food, employment and income). Unfortunately, even with such recognition, coupled with many reforms initiated by government and supported by various development partners who have invested huge sums of money into the sector, agricultural productivity and profitability has remained extremely low. Most rural Ugandans still languish in abject poverty, hunger, malnutrition, vulnerability and powerlessness. This study has established that, while the agricultural sector in Uganda suffers from enormous institutional, technological, market, research and land-oriented challenges, if all these factors are kept constant, an effective community-based approach is capable of facilitating effective planning, implementation, management, monitoring, and evaluation of agricultural reforms, for increased agricultural productivity and profitability hence the improved quality of life of Ugandans. A qualitative elicitation interviewing technique involving in-depth discussions with agricultural extension workers, community development officers, representatives from NGOs, local farmers, farmers’ associations, local and religious leaders as well as key persons from the Ministry of Agriculture and other line ministries was conducted. The participants were selected through chain referrals until the level of theoretical saturation. In addition, directed field observations, document analysis and key informant interviews with other respondents selected through theoretical sampling enhanced the robustness of data acquisition methods. Group-based participatory data analysis and reflexive pragmatism also enhanced the rigour and quality of research findings intended to balance the knowledge generated from the recognised scientific audience and the views of the important but unknown “knowledge generators” (the local experts).The key findings indicate that, historically and currently, agriculture has been and is the predominant community development activity at household, community and national levels in Uganda. It is also anticipated that agriculture will remain a major contributor to the national economic development of Uganda even over the next hundred years. The efforts by government and its development partners to transform the sector are therefore justified by the sector’s strategic importance. It has been found out that although the sector faces huge challenges, there are numerous opportunities for the sector to become a driver of Uganda’s socio-economic development. A community-based model has been proposed as a viable option for facilitating faster agricultural development in Uganda, where technocratic developers tend to impose development reforms on local people. With the traditional approaches to planning and implementation, it was thought that local people do not know what they want, they are illiterate and ignorant and therefore incapable of driving government crafted programmes for effective change. From the proposed model, government technocratic development agents should engage local farmers who practically experience poverty, powerlessness, hunger, malnutrition and vulnerability, to jointly craft effective agricultural reforms that are not only life-changing, but also relevant and sustainable within the confines of community needs. Under this approach, the designers, planners, implementers, monitors, and evaluators, whether of government-initiated reforms, or initiatives from other development agencies, should learn to treat local people as subjects of the community development processes, as opposed to the traditional top-down mechanisms which view them as objects to be used and abused. The involvement of local experts during the planning, implementation, and management of development reforms, should cease to be applied as merely routine fulfilment of donor conditionality and requirements. Rather, the participation of local farmers during all phases of community programming should become intentional and consciously aimed at empowering local people to actively participate in the making of decisions critical to their own development.
- Full Text:
- Date Issued: 2020
- Authors: Kiggundu, Joseph
- Date: 2020
- Subjects: Agricultural development projects -- Uganda
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10948/48654 , vital:41056
- Description: This study intended to design a community-based model for agricultural development in Uganda, using Kumi and Gomba districts as case studies. The surge in attention towards community-based development is attributed to the growing challenges posed by the traditional approaches to community development, dominated by top-down mechanisms during the planning, implementation, management, monitoring, and evaluation phases of community development programmes. Driven by Robert Chambers and Conway’s theoretical perspectives, this study discusses that a community-based model is capable of filling knowledge gaps, not only in scientific research, but also in the community development process of many developing nations, such as Uganda. In this study particularly, a community-based approach has been identified as the better option in aiding and facilitating the current government efforts to transform the agricultural sector from its predominantly subsistence nature to commercial orientation. It has been acknowledged that the Ugandan government and its development partners do realise the significant role agriculture plays in directly or indirectly impacting the social welfare of Ugandans. The agricultural sector has been identified as a major source of livelihood for most rural Ugandans (over 95% depend on it for food, employment and income). Unfortunately, even with such recognition, coupled with many reforms initiated by government and supported by various development partners who have invested huge sums of money into the sector, agricultural productivity and profitability has remained extremely low. Most rural Ugandans still languish in abject poverty, hunger, malnutrition, vulnerability and powerlessness. This study has established that, while the agricultural sector in Uganda suffers from enormous institutional, technological, market, research and land-oriented challenges, if all these factors are kept constant, an effective community-based approach is capable of facilitating effective planning, implementation, management, monitoring, and evaluation of agricultural reforms, for increased agricultural productivity and profitability hence the improved quality of life of Ugandans. A qualitative elicitation interviewing technique involving in-depth discussions with agricultural extension workers, community development officers, representatives from NGOs, local farmers, farmers’ associations, local and religious leaders as well as key persons from the Ministry of Agriculture and other line ministries was conducted. The participants were selected through chain referrals until the level of theoretical saturation. In addition, directed field observations, document analysis and key informant interviews with other respondents selected through theoretical sampling enhanced the robustness of data acquisition methods. Group-based participatory data analysis and reflexive pragmatism also enhanced the rigour and quality of research findings intended to balance the knowledge generated from the recognised scientific audience and the views of the important but unknown “knowledge generators” (the local experts).The key findings indicate that, historically and currently, agriculture has been and is the predominant community development activity at household, community and national levels in Uganda. It is also anticipated that agriculture will remain a major contributor to the national economic development of Uganda even over the next hundred years. The efforts by government and its development partners to transform the sector are therefore justified by the sector’s strategic importance. It has been found out that although the sector faces huge challenges, there are numerous opportunities for the sector to become a driver of Uganda’s socio-economic development. A community-based model has been proposed as a viable option for facilitating faster agricultural development in Uganda, where technocratic developers tend to impose development reforms on local people. With the traditional approaches to planning and implementation, it was thought that local people do not know what they want, they are illiterate and ignorant and therefore incapable of driving government crafted programmes for effective change. From the proposed model, government technocratic development agents should engage local farmers who practically experience poverty, powerlessness, hunger, malnutrition and vulnerability, to jointly craft effective agricultural reforms that are not only life-changing, but also relevant and sustainable within the confines of community needs. Under this approach, the designers, planners, implementers, monitors, and evaluators, whether of government-initiated reforms, or initiatives from other development agencies, should learn to treat local people as subjects of the community development processes, as opposed to the traditional top-down mechanisms which view them as objects to be used and abused. The involvement of local experts during the planning, implementation, and management of development reforms, should cease to be applied as merely routine fulfilment of donor conditionality and requirements. Rather, the participation of local farmers during all phases of community programming should become intentional and consciously aimed at empowering local people to actively participate in the making of decisions critical to their own development.
- Full Text:
- Date Issued: 2020
Towards a reconstruction of Marion Island’s glacial history
- Authors: Rudolph, Elizabeth
- Date: 2020
- Subjects: Glacial landforms Geomorphological mapping
- Language: English
- Type: Thesis , Doctoral , PhD (Geography)
- Identifier: http://hdl.handle.net/10353/18531 , vital:42581
- Description: Southern Hemisphere glacial chronologies can provide valuable insights into interactions between glaciation and past climate changes. The sub-Antarctic Islands provide a valuable terrestrial record of glacial chronologies for the Southern Hemisphere, since they are unique, not only in size and topography, but also in oceanic situation when compared to other continental landmasses (e.g. Antarctica or Patagonia). On sub-Antarctic Marion Island, southern Indian Ocean, the exact timing and extent of the local Last Glacial Maximum is not yet known as glacial reconstructions have mostly been based on palynological proxies and relative-age dating techniques. This study presents 29 cosmogenic 36Cl exposure ages of deglaciated bedrock surfaces and moraine deposits from Marion Island. In addition, a comprehensive glacial-geomorphological map, which in conjunction with exposure ages provide improved temporal and spatial constraints for the island’s glacial history. Results show that the ice reached a local Last Glacial Maximum before 56 ka ago and retreated, with minor stillstands, until ~17 ka. This early deglaciation left island surfaces below 850 m a.s.l. ice-free after ~19 ka, and any subsequent advances during the Late Glacial or Holocene cooling periods would have been restricted to the interior. This glacial chronology is similar to that of some other sub-Antarctic Islands (e.g. the Kerguelen archipelago, Auckland and Campbell islands, and possibly South Georgia) and a number of other Southern Hemisphere glaciers (e.g. in Patagonia and New Zealand) and adds to evidence that suggest the Southern Hemisphere was in a glacial maximum earlier than the global LGM. A combination of climatic drivers such as declining temperatures, a northward migration of oceanic fronts and the Southern Hemisphere Westerly Winds (causing precipitation changes), as well as the physiography of Marion Island, created optimal conditions for glacier growth during Marine Isotope Stage 4 (MIS 4; ~65 ka ago) instead of the global LGM in MIS 2 (~18 ka). These findings redefine the glacial history of Marion Island, and have implications for future investigations on post-glacial landscape development and ecological succession.
- Full Text:
- Date Issued: 2020
- Authors: Rudolph, Elizabeth
- Date: 2020
- Subjects: Glacial landforms Geomorphological mapping
- Language: English
- Type: Thesis , Doctoral , PhD (Geography)
- Identifier: http://hdl.handle.net/10353/18531 , vital:42581
- Description: Southern Hemisphere glacial chronologies can provide valuable insights into interactions between glaciation and past climate changes. The sub-Antarctic Islands provide a valuable terrestrial record of glacial chronologies for the Southern Hemisphere, since they are unique, not only in size and topography, but also in oceanic situation when compared to other continental landmasses (e.g. Antarctica or Patagonia). On sub-Antarctic Marion Island, southern Indian Ocean, the exact timing and extent of the local Last Glacial Maximum is not yet known as glacial reconstructions have mostly been based on palynological proxies and relative-age dating techniques. This study presents 29 cosmogenic 36Cl exposure ages of deglaciated bedrock surfaces and moraine deposits from Marion Island. In addition, a comprehensive glacial-geomorphological map, which in conjunction with exposure ages provide improved temporal and spatial constraints for the island’s glacial history. Results show that the ice reached a local Last Glacial Maximum before 56 ka ago and retreated, with minor stillstands, until ~17 ka. This early deglaciation left island surfaces below 850 m a.s.l. ice-free after ~19 ka, and any subsequent advances during the Late Glacial or Holocene cooling periods would have been restricted to the interior. This glacial chronology is similar to that of some other sub-Antarctic Islands (e.g. the Kerguelen archipelago, Auckland and Campbell islands, and possibly South Georgia) and a number of other Southern Hemisphere glaciers (e.g. in Patagonia and New Zealand) and adds to evidence that suggest the Southern Hemisphere was in a glacial maximum earlier than the global LGM. A combination of climatic drivers such as declining temperatures, a northward migration of oceanic fronts and the Southern Hemisphere Westerly Winds (causing precipitation changes), as well as the physiography of Marion Island, created optimal conditions for glacier growth during Marine Isotope Stage 4 (MIS 4; ~65 ka ago) instead of the global LGM in MIS 2 (~18 ka). These findings redefine the glacial history of Marion Island, and have implications for future investigations on post-glacial landscape development and ecological succession.
- Full Text:
- Date Issued: 2020
Towards decolonizing a local university’s business school’s masters in business administration
- Authors: Du Preez, Daniella
- Date: 2020
- Subjects: Decolonization -- Education (Higher) -- Curricula
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: http://hdl.handle.net/10948/47735 , vital:40330
- Description: 2015 marked the year students went to great lengths to call on Higher Education institutions and their educators for a decolonised curriculum. This behaviour is attributable to the conditions that they had do study in. Buildings and campuses have colonial names, lectures mostly include White people’s intellectual contributions. Colonialism included colonial knowledge and denied African practices and existence. For centuries African philosophy was excluded and marginalised, which caused much pain and inhumanity. Higher education institutions remain detached from the developmental changes of African communities. Silence in academic disciplines was overwhelming Eurocentric / Westernised. Transformation of Higher Education curriculum in South Africa requires inclusion of African philosophy, and acknowledgement and respect for other cultures’ values, languages and knowledge. Educators play a pivotal role in transferring knowledge to students and producing the leaders for the future. Colonisation has ruled for decades and the effects thereof are still felt in various aspects of life. The education platform can break colonialism and if allowed, bring restoration to indigenous people. South African universities can contribute to society in transforming and decolonising curriculum and giving indigenous people and knowledge their rightful place. An education system free from colonialism positions students to add value to society as their worldviews are taken into consideration. This paper investigates the factors that could lead to a decolonised curriculum. Higher Education institutions have a role to play in ensuring a University free form colonial power and inequality. Decolonisation of the intellectual landscape will result in the decolonisation of the mind.
- Full Text:
- Date Issued: 2020
- Authors: Du Preez, Daniella
- Date: 2020
- Subjects: Decolonization -- Education (Higher) -- Curricula
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: http://hdl.handle.net/10948/47735 , vital:40330
- Description: 2015 marked the year students went to great lengths to call on Higher Education institutions and their educators for a decolonised curriculum. This behaviour is attributable to the conditions that they had do study in. Buildings and campuses have colonial names, lectures mostly include White people’s intellectual contributions. Colonialism included colonial knowledge and denied African practices and existence. For centuries African philosophy was excluded and marginalised, which caused much pain and inhumanity. Higher education institutions remain detached from the developmental changes of African communities. Silence in academic disciplines was overwhelming Eurocentric / Westernised. Transformation of Higher Education curriculum in South Africa requires inclusion of African philosophy, and acknowledgement and respect for other cultures’ values, languages and knowledge. Educators play a pivotal role in transferring knowledge to students and producing the leaders for the future. Colonisation has ruled for decades and the effects thereof are still felt in various aspects of life. The education platform can break colonialism and if allowed, bring restoration to indigenous people. South African universities can contribute to society in transforming and decolonising curriculum and giving indigenous people and knowledge their rightful place. An education system free from colonialism positions students to add value to society as their worldviews are taken into consideration. This paper investigates the factors that could lead to a decolonised curriculum. Higher Education institutions have a role to play in ensuring a University free form colonial power and inequality. Decolonisation of the intellectual landscape will result in the decolonisation of the mind.
- Full Text:
- Date Issued: 2020
Towards development of a malaria diagnostic: Generation, screening and validation of novel aptamers recognising Plasmodium falciparum lactate dehydrogenase
- Authors: Frith, Kelly-Anne
- Date: 2020
- Subjects: Plasmodium falciparum , Malaria -- Chemotherapy , Oligonucleotides , Lactate dehydrogenase , Biochemical markers , Systematic evolution of ligands through exponential enrichment (SELEX)
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/142247 , vital:38062
- Description: Malaria, caused by infection with the Plasmodium parasite, is one of the leading causes of death in under-developed countries. Early detection is crucial for the effective treatment of malaria, particularly in cases where infection is due to Plasmodium falciparum. There is, therefore, an enduring need for portable, sensitive, reliable, accurate, durable, self-validating and cost-effective techniques for the rapid detection of malaria. Moreover, there is a demand to distinguish between various infectious species causing malaria. Research in the area of malarial biomarkers has identified a unique, species-specific, epitope of P. falciparum lactate dehydrogenase (PfLDH), enhancing prospects for the development of diagnostics capable of identifying the species causing malarial infection. In recent years, improvements have been made towards the development of rapid diagnostic tests for detecting malarial biomarkers. Owing to their low cost, ease of labeling, and high thermal stability (relative to antibodies), the development and synthesis of aptamers that target the malarial lactate dehydrogenase represents one of the key innovations in the field of rapid diagnostics for malaria. This study explored the generation of aptamers that specifically target P. falciparum. Two sets of aptamers with diagnostically-supportive functions were generated independently, through parallel SELEX of recombinantly-expressed, full-length Plasmodium falciparum lactate dehydrogenase (rPfLDH), and an oligopeptide comprising the P. falciparum-specific epitope on lactate dehydrogenase (LDHp). The latter offers a promising solution for generating aptamers capable of binding with high specificity to P. falciparum. In this work, an rLDH class of aptamers was generated when SELEX was performed using the full-length rPfLDH protein as the target and the LDHp class of aptamers was generated when SELEX was performed using the oligopeptide LDHp as a target. Aptamers were successfully generated through the process of SELEX (systematic evolution of ligands through exponential enrichment) following the study and application of several optimisation steps, particularly during the amplification stage of SELEX. Optimisation steps included the study of improvements in PCR conditions; role of surfactants (Triton-X), modifying the PCR clean-up protocol; and agarose gel excision. Structurally-relevant moieties with particular consensus sequences (GGTAG and GGCG) were found in aptamers both reported here and previously published, confirming their importance in recognition of the target. Novel moieties particular to this work (ATTAT and poly-A stretches) were identified. Clades of consensus sequences were identified in both the rLDH and LDHp groups of aptamers, where sequences in the rLDH clade did not show preferential binding to rPfLDH while those in the LDHp clade (particularly LDHp 3 and 18) were able to recognise and bind only LDHp. Of the 19 sequences returned from the parallel SELEX procedures for rPfLDH (11 sequences) and LDHp (8 sequences), six rPfLDH and all eight LDHp sequences underwent preliminary screening and those with low responses eliminated. Of the eight LDHp-targeting aptamer sequences, five were preliminarily shown to bind to LDHp, whereas only two rPfLDH-targeting sequences were shown to bind to the target (rLDH 4 and 7). To this small selection of rPfLDH oligonucleotide sequences, two more (rLDH 1 and 15) were chosen for further study based on their sequences, secondary and predicted tertiary conformations. Sequences chosen for further study were therefore: rLDH 1, 4, 7 and 15 in the rLDH class, and LDHp 1, 3, 11, 14 and 18 in the LDHp class. Binding properties of the aptamers towards their targets were investigated using enzyme-linked oligonucleotide assays (ELONA), fluorophore-linked oligonucleotide assays (FLONA), electromobility shift assays (EMSA), surface plasmon resonance (SPR), and GelRed dissociation assays, while applications towards aptasensors were explored using electrochemical impedance spectroscopy (EIS) and fluorescent microscopy. Some inconsistencies were seen for specific aptamer to target binding interactions using specific techniques; however, generally, binding to the targets was observed across the techniques assessed. These varied responses demonstrate the need to screen and validate aptamers using a variety of techniques and platforms not necessarily specific for the proposed application. From the aptamer binding screening studies using ELONA, the most promising aptamers generated were identified as LDHp 11, rLDH 4, rLDH 7 and rLDH 15. Aptamer rLDH 4, which was generated against rPfLDH, exhibited preferential and specific binding to the lactate dehydrogenase from P. falciparum, over the recombinantly-expressed lactate dehydrogenase from Plasmodium vivax (rPvLDH), albeit with lowered responses compared to LDHp 11 in ELONA and EMSA studies. However, in kinetic ELONA studies rLDH 4 showed binding to both rPfLDH and rPvLDH. Aptamer rLDH 7 showed high affinity for rPfLDH and rPvLDH in kinetic studies using ELONA. However, screening studies with ELONA indicates that aptamer rLDH 7 may not be suitable for diagnostic tests in serum samples given its non-specific binding to human serum albumin (HSA). Aptamer rLDH 15 exhibited species specificity for rPfLDH in screening studies using ELONA but showed affinity towards rPvLDH (albeit lower relative to its affinity for rPfLDH) in kinetic studies using ELONA. LDHp 11, generated against the PfLDH peptide, showed a clear preference for rPfLDH when compared to rPvLDH and other control proteins, in both sets of ELONA studies conducted, as well as EMSA, thus possessing a strong ability to identify the presence of Plasmodium falciparum owing to its generation against the species-specific epitope. While LDHp 1 demonstrated binding to plasmodial LDH in a flow-through system (SPR), so reiterating ELONA responses, it did not perform well in the remaining methodologies. Aptamers rLDH 1 and 15 and LDHp 3, 14 and 18 exhibited a mixed set of results throughout the target protein screening analyses and were, thus, not considered for selective binding in P. falciparum parasite bodies. In studies aimed at exploring biosensor assemblies utilising the developed aptamers, both rLDH 4 and LDHp 11, along with rLDH 7, LDHp 1 and pL1, demonstrated in situ binding to the native PfLDH in fluorescent microscopy. LDHp 11 exhibited FITC-based fluorescence equivalent to the anti-rPfLDHp IgY antibody in confocal fluorescent microscopy indicating superior binding to the native PfLDH compared to the remaining aptamers. An examination of electrochemical impedance as a platform for a biosensor assembly did not, in these studies, exhibit the required sensitivity using physiologically relevant concentrations of analyte expected for pLDH following infection with Plasmodium spp. Malstat/LDH activity was explored for application in a colorimetric aptasensor. A decrease in both rPfLDH and rPvLDH activity was observed following incubation with the tested aptamers, but rLDH 1, rLDH 7 and LDHp 14 did not exhibit similar decreases in rPvLDH activity. Aptamers rLDH 1, 4 and 7 and LDHp 11 and 14 were, therefore, not selected as candidates for LDH capture in LDH activity-based diagnostic devices for P. falciparum. The decreases in pLDH activity in the presence of aptamers could hold promise as direct or antagonistic malaria therapeutic agents. Preliminary studies on the application of aptamers as malaria therapeutic agents, while of interest, should be viewed with due caution given the challenges of aptamers reaching the intracellular native plasmodial LDH hosted within the red blood cells. In conclusion, this work has shown the ability of the LDHp 11 aptamer, generated in these studies, to selectively bind rPfLDH over rPvLDH, and to bind to the native PfLDH in fluorescent microscopy, indicating that this aptamer holds promise as a biorecognition element in malaria biosensors and other diagnostic devices for the detection, and differentiation, of P. falciparum and P. vivax. The use of a species-specific epitope of P. falciparum as a target in aptamer generation paves the way for similar such studies aimed at generating aptamers with species selectivity for other Plasmodium species.
- Full Text:
- Date Issued: 2020
- Authors: Frith, Kelly-Anne
- Date: 2020
- Subjects: Plasmodium falciparum , Malaria -- Chemotherapy , Oligonucleotides , Lactate dehydrogenase , Biochemical markers , Systematic evolution of ligands through exponential enrichment (SELEX)
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/142247 , vital:38062
- Description: Malaria, caused by infection with the Plasmodium parasite, is one of the leading causes of death in under-developed countries. Early detection is crucial for the effective treatment of malaria, particularly in cases where infection is due to Plasmodium falciparum. There is, therefore, an enduring need for portable, sensitive, reliable, accurate, durable, self-validating and cost-effective techniques for the rapid detection of malaria. Moreover, there is a demand to distinguish between various infectious species causing malaria. Research in the area of malarial biomarkers has identified a unique, species-specific, epitope of P. falciparum lactate dehydrogenase (PfLDH), enhancing prospects for the development of diagnostics capable of identifying the species causing malarial infection. In recent years, improvements have been made towards the development of rapid diagnostic tests for detecting malarial biomarkers. Owing to their low cost, ease of labeling, and high thermal stability (relative to antibodies), the development and synthesis of aptamers that target the malarial lactate dehydrogenase represents one of the key innovations in the field of rapid diagnostics for malaria. This study explored the generation of aptamers that specifically target P. falciparum. Two sets of aptamers with diagnostically-supportive functions were generated independently, through parallel SELEX of recombinantly-expressed, full-length Plasmodium falciparum lactate dehydrogenase (rPfLDH), and an oligopeptide comprising the P. falciparum-specific epitope on lactate dehydrogenase (LDHp). The latter offers a promising solution for generating aptamers capable of binding with high specificity to P. falciparum. In this work, an rLDH class of aptamers was generated when SELEX was performed using the full-length rPfLDH protein as the target and the LDHp class of aptamers was generated when SELEX was performed using the oligopeptide LDHp as a target. Aptamers were successfully generated through the process of SELEX (systematic evolution of ligands through exponential enrichment) following the study and application of several optimisation steps, particularly during the amplification stage of SELEX. Optimisation steps included the study of improvements in PCR conditions; role of surfactants (Triton-X), modifying the PCR clean-up protocol; and agarose gel excision. Structurally-relevant moieties with particular consensus sequences (GGTAG and GGCG) were found in aptamers both reported here and previously published, confirming their importance in recognition of the target. Novel moieties particular to this work (ATTAT and poly-A stretches) were identified. Clades of consensus sequences were identified in both the rLDH and LDHp groups of aptamers, where sequences in the rLDH clade did not show preferential binding to rPfLDH while those in the LDHp clade (particularly LDHp 3 and 18) were able to recognise and bind only LDHp. Of the 19 sequences returned from the parallel SELEX procedures for rPfLDH (11 sequences) and LDHp (8 sequences), six rPfLDH and all eight LDHp sequences underwent preliminary screening and those with low responses eliminated. Of the eight LDHp-targeting aptamer sequences, five were preliminarily shown to bind to LDHp, whereas only two rPfLDH-targeting sequences were shown to bind to the target (rLDH 4 and 7). To this small selection of rPfLDH oligonucleotide sequences, two more (rLDH 1 and 15) were chosen for further study based on their sequences, secondary and predicted tertiary conformations. Sequences chosen for further study were therefore: rLDH 1, 4, 7 and 15 in the rLDH class, and LDHp 1, 3, 11, 14 and 18 in the LDHp class. Binding properties of the aptamers towards their targets were investigated using enzyme-linked oligonucleotide assays (ELONA), fluorophore-linked oligonucleotide assays (FLONA), electromobility shift assays (EMSA), surface plasmon resonance (SPR), and GelRed dissociation assays, while applications towards aptasensors were explored using electrochemical impedance spectroscopy (EIS) and fluorescent microscopy. Some inconsistencies were seen for specific aptamer to target binding interactions using specific techniques; however, generally, binding to the targets was observed across the techniques assessed. These varied responses demonstrate the need to screen and validate aptamers using a variety of techniques and platforms not necessarily specific for the proposed application. From the aptamer binding screening studies using ELONA, the most promising aptamers generated were identified as LDHp 11, rLDH 4, rLDH 7 and rLDH 15. Aptamer rLDH 4, which was generated against rPfLDH, exhibited preferential and specific binding to the lactate dehydrogenase from P. falciparum, over the recombinantly-expressed lactate dehydrogenase from Plasmodium vivax (rPvLDH), albeit with lowered responses compared to LDHp 11 in ELONA and EMSA studies. However, in kinetic ELONA studies rLDH 4 showed binding to both rPfLDH and rPvLDH. Aptamer rLDH 7 showed high affinity for rPfLDH and rPvLDH in kinetic studies using ELONA. However, screening studies with ELONA indicates that aptamer rLDH 7 may not be suitable for diagnostic tests in serum samples given its non-specific binding to human serum albumin (HSA). Aptamer rLDH 15 exhibited species specificity for rPfLDH in screening studies using ELONA but showed affinity towards rPvLDH (albeit lower relative to its affinity for rPfLDH) in kinetic studies using ELONA. LDHp 11, generated against the PfLDH peptide, showed a clear preference for rPfLDH when compared to rPvLDH and other control proteins, in both sets of ELONA studies conducted, as well as EMSA, thus possessing a strong ability to identify the presence of Plasmodium falciparum owing to its generation against the species-specific epitope. While LDHp 1 demonstrated binding to plasmodial LDH in a flow-through system (SPR), so reiterating ELONA responses, it did not perform well in the remaining methodologies. Aptamers rLDH 1 and 15 and LDHp 3, 14 and 18 exhibited a mixed set of results throughout the target protein screening analyses and were, thus, not considered for selective binding in P. falciparum parasite bodies. In studies aimed at exploring biosensor assemblies utilising the developed aptamers, both rLDH 4 and LDHp 11, along with rLDH 7, LDHp 1 and pL1, demonstrated in situ binding to the native PfLDH in fluorescent microscopy. LDHp 11 exhibited FITC-based fluorescence equivalent to the anti-rPfLDHp IgY antibody in confocal fluorescent microscopy indicating superior binding to the native PfLDH compared to the remaining aptamers. An examination of electrochemical impedance as a platform for a biosensor assembly did not, in these studies, exhibit the required sensitivity using physiologically relevant concentrations of analyte expected for pLDH following infection with Plasmodium spp. Malstat/LDH activity was explored for application in a colorimetric aptasensor. A decrease in both rPfLDH and rPvLDH activity was observed following incubation with the tested aptamers, but rLDH 1, rLDH 7 and LDHp 14 did not exhibit similar decreases in rPvLDH activity. Aptamers rLDH 1, 4 and 7 and LDHp 11 and 14 were, therefore, not selected as candidates for LDH capture in LDH activity-based diagnostic devices for P. falciparum. The decreases in pLDH activity in the presence of aptamers could hold promise as direct or antagonistic malaria therapeutic agents. Preliminary studies on the application of aptamers as malaria therapeutic agents, while of interest, should be viewed with due caution given the challenges of aptamers reaching the intracellular native plasmodial LDH hosted within the red blood cells. In conclusion, this work has shown the ability of the LDHp 11 aptamer, generated in these studies, to selectively bind rPfLDH over rPvLDH, and to bind to the native PfLDH in fluorescent microscopy, indicating that this aptamer holds promise as a biorecognition element in malaria biosensors and other diagnostic devices for the detection, and differentiation, of P. falciparum and P. vivax. The use of a species-specific epitope of P. falciparum as a target in aptamer generation paves the way for similar such studies aimed at generating aptamers with species selectivity for other Plasmodium species.
- Full Text:
- Date Issued: 2020
Towards realising the benefits of citizen participation in environmental monitoring: a case study in an Eastern Cape natural resource management programme
- Authors: Mtati, Nosiseko
- Date: 2020
- Subjects: Tsitsa Project , Rural development projects -- South Africa -- Eastern Cape , Environmental monitoring -- South Africa -- Tsitsa River , Environmental monitoring -- Citizen participation -- South Africa -- Tsitsa River , Water supply, Agricultural -- South Africa -- Tsitsa River , Environmental education -- South Africa
- Language: English
- Type: text , Thesis , Masters , MEd
- Identifier: http://hdl.handle.net/10962/167562 , vital:41492
- Description: The Tsitsa Project focusses on land use management and rural livelihoods in the Tsitsa River catchment in the Eastern Cape Province of South Africa. It is funded by the Department of Environmental Affairs and the environmental monitoring initiative is implemented by Rhodes University, where I am employed as the catchment coordinator. This study explores the environmental monitoring initiative within the bigger Tsitsa Project. Community members in the catchment monitor sediment transportation in the Tsitsa River and its tributaries, which originally became of interest because it is proposed that a dam (Ntabelanga Dam) be established here. This study aims to understand citizen environmental monitoring in the Tsitsa Project; what the project managers regarded as benefits; and how the monitors themselves perceived benefits of participating as monitors. A realist approach was followed, in order to understand the connections between the context and the mechanisms in the project, and how these combined to result in the outcomes observed. Realist research emphasises the importance of context in shaping outcomes such as the achieved benefits of citizen monitoring. Data was collected using a case study method, where each individual monitor and their particular context, was regarded as a case. Semistructured interviews were conducted with 17 monitors and five Tsitsa Project staff; this was supported by field notes and the reviewing of project documents including field reports. The realist analysis looked at the context of the monitors in general and the mechanisms applied by the project in recruiting, training and managing the monitors. A second layer of mechanisms was identified as those responses from the monitors to what the project was introducing to them. Outcomes were both positive and negative, including how long monitors remained in the initiative, what benefits they derived from the process, and what potential benefits they did not achieve. This included lost opportunities to provide recognition for skills and experience gained. Recommendations are made regarding the recruitment, training and management of monitors, to optimise benefits for the monitors, the host institution and the initiative’s staff. The study is significant because of its particular yet representative characteristics and it will assist both the Tsitsa Project, which aims to expand its citizen environmental monitoring initiative, as well as wider Natural Resource Management Programmes in South Africa. It is also hoped that it will contribute to the literature on environmental monitoring as a little researched form of citizen science globally.
- Full Text:
- Date Issued: 2020
- Authors: Mtati, Nosiseko
- Date: 2020
- Subjects: Tsitsa Project , Rural development projects -- South Africa -- Eastern Cape , Environmental monitoring -- South Africa -- Tsitsa River , Environmental monitoring -- Citizen participation -- South Africa -- Tsitsa River , Water supply, Agricultural -- South Africa -- Tsitsa River , Environmental education -- South Africa
- Language: English
- Type: text , Thesis , Masters , MEd
- Identifier: http://hdl.handle.net/10962/167562 , vital:41492
- Description: The Tsitsa Project focusses on land use management and rural livelihoods in the Tsitsa River catchment in the Eastern Cape Province of South Africa. It is funded by the Department of Environmental Affairs and the environmental monitoring initiative is implemented by Rhodes University, where I am employed as the catchment coordinator. This study explores the environmental monitoring initiative within the bigger Tsitsa Project. Community members in the catchment monitor sediment transportation in the Tsitsa River and its tributaries, which originally became of interest because it is proposed that a dam (Ntabelanga Dam) be established here. This study aims to understand citizen environmental monitoring in the Tsitsa Project; what the project managers regarded as benefits; and how the monitors themselves perceived benefits of participating as monitors. A realist approach was followed, in order to understand the connections between the context and the mechanisms in the project, and how these combined to result in the outcomes observed. Realist research emphasises the importance of context in shaping outcomes such as the achieved benefits of citizen monitoring. Data was collected using a case study method, where each individual monitor and their particular context, was regarded as a case. Semistructured interviews were conducted with 17 monitors and five Tsitsa Project staff; this was supported by field notes and the reviewing of project documents including field reports. The realist analysis looked at the context of the monitors in general and the mechanisms applied by the project in recruiting, training and managing the monitors. A second layer of mechanisms was identified as those responses from the monitors to what the project was introducing to them. Outcomes were both positive and negative, including how long monitors remained in the initiative, what benefits they derived from the process, and what potential benefits they did not achieve. This included lost opportunities to provide recognition for skills and experience gained. Recommendations are made regarding the recruitment, training and management of monitors, to optimise benefits for the monitors, the host institution and the initiative’s staff. The study is significant because of its particular yet representative characteristics and it will assist both the Tsitsa Project, which aims to expand its citizen environmental monitoring initiative, as well as wider Natural Resource Management Programmes in South Africa. It is also hoped that it will contribute to the literature on environmental monitoring as a little researched form of citizen science globally.
- Full Text:
- Date Issued: 2020
Towards the development of a bio-fertiliser using mixed liquor from high rate algal oxidation ponds
- Authors: Masudi, Wiya Léon
- Date: 2020
- Subjects: Biofertilizers , Microalgae - Biotechnology , Algae -- Culture
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/142969 , vital:38181
- Description: Mixed liquor includes consortia of microalgae and bacteria produced in high rate algal oxidation ponds (HRAOPs). The consortia of microalgae and bacteria occur as flocs called microalgae-bacterial flocs (MaB-flocs). This study aimed to source bacteria from MaB-flocs generated in HRAOPs and, after isolation and identification, evaluate their potential as plant growth promoting (PGP) microorganisms. Twelve bacterial strains namely ECCN 1b, ECCN 2b, ECCN 3b, ECCN 4b, ECCN 5b, ECCN 6b, ECCN 7b, ECCN 8b, ECCN 9b, ECCN 10b, ECCN 11b, and ECCN 12b were successfully isolated and their molecular identity established using amplified 16S rRNA gene sequence analysis that was compared to sequences deposited in the NCBI gene database. Blast analysis identified these isolates at the genus level as Bacillus strain ECCN 1b, Fictibacillus strain ECCN 2b, Bacillus strain ECCN 3b, Aeromonas strain ECCN 4b, Exiguobacterium strain ECCN 5b, Arthrobacter strain ECCN 6b, Enterobacter strain ECCN 7b, Exiguobacterium strain ECCN 8b, Microbacterium strain ECCN 9b, Pseudomonas ECCN strain 10b, Ancylobacter strain ECCN 11b and Microbacterium strain ECCN 12b. These isolates were able to grow in nutrient broth in a pH range between 6 and 10, with the best growth achieved at pH 8 to 9. The results on the use of carbon substrate revealed that 5 strains including Arthrobacter strain ECCN 6b, Aeromonas strain ECCN 4b, Pseudomonas strain ECCN 10b, Enterobacter strain ECCN 7b and Bacillus strain ECCN 3b were capable of using glucose, sucrose and mannitol. No faecal coliforms were found. However, of the 12 isolates screened for bio-fertilisation potential, Bacillus strain ECCN 1b, Fictibacillus strain ECCN 2b, Bacillus strain ECCN 3b, Aeromonas strain ECCN 4b, Exiguobacterium strain ECCN 5b, Arthrobacter strain ECCN 6b, Enterobacter ECCN strain 7b, Exiguobacterium strain ECCN 8b and Pseudomonas strain ECCN 10b showed multifunctional plant growth promoting (PGP) potential. The potential for PGP included the production of ammonium-N, solubilisation of phosphate-P and potassium-K, oxidation of Mn and production of auxin, indole-3-acetic acid (IAA). Results are discussed in terms of the amount or concentration (mg L-1) of plant essential nutrient and growth regulator produced by these isolated bacteria. Even so, further studies are needed to test and confirm the bio-fertiliser and plant growth promoting activity of these strains in pot trials and field experiments, or both.
- Full Text:
- Date Issued: 2020
- Authors: Masudi, Wiya Léon
- Date: 2020
- Subjects: Biofertilizers , Microalgae - Biotechnology , Algae -- Culture
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/142969 , vital:38181
- Description: Mixed liquor includes consortia of microalgae and bacteria produced in high rate algal oxidation ponds (HRAOPs). The consortia of microalgae and bacteria occur as flocs called microalgae-bacterial flocs (MaB-flocs). This study aimed to source bacteria from MaB-flocs generated in HRAOPs and, after isolation and identification, evaluate their potential as plant growth promoting (PGP) microorganisms. Twelve bacterial strains namely ECCN 1b, ECCN 2b, ECCN 3b, ECCN 4b, ECCN 5b, ECCN 6b, ECCN 7b, ECCN 8b, ECCN 9b, ECCN 10b, ECCN 11b, and ECCN 12b were successfully isolated and their molecular identity established using amplified 16S rRNA gene sequence analysis that was compared to sequences deposited in the NCBI gene database. Blast analysis identified these isolates at the genus level as Bacillus strain ECCN 1b, Fictibacillus strain ECCN 2b, Bacillus strain ECCN 3b, Aeromonas strain ECCN 4b, Exiguobacterium strain ECCN 5b, Arthrobacter strain ECCN 6b, Enterobacter strain ECCN 7b, Exiguobacterium strain ECCN 8b, Microbacterium strain ECCN 9b, Pseudomonas ECCN strain 10b, Ancylobacter strain ECCN 11b and Microbacterium strain ECCN 12b. These isolates were able to grow in nutrient broth in a pH range between 6 and 10, with the best growth achieved at pH 8 to 9. The results on the use of carbon substrate revealed that 5 strains including Arthrobacter strain ECCN 6b, Aeromonas strain ECCN 4b, Pseudomonas strain ECCN 10b, Enterobacter strain ECCN 7b and Bacillus strain ECCN 3b were capable of using glucose, sucrose and mannitol. No faecal coliforms were found. However, of the 12 isolates screened for bio-fertilisation potential, Bacillus strain ECCN 1b, Fictibacillus strain ECCN 2b, Bacillus strain ECCN 3b, Aeromonas strain ECCN 4b, Exiguobacterium strain ECCN 5b, Arthrobacter strain ECCN 6b, Enterobacter ECCN strain 7b, Exiguobacterium strain ECCN 8b and Pseudomonas strain ECCN 10b showed multifunctional plant growth promoting (PGP) potential. The potential for PGP included the production of ammonium-N, solubilisation of phosphate-P and potassium-K, oxidation of Mn and production of auxin, indole-3-acetic acid (IAA). Results are discussed in terms of the amount or concentration (mg L-1) of plant essential nutrient and growth regulator produced by these isolated bacteria. Even so, further studies are needed to test and confirm the bio-fertiliser and plant growth promoting activity of these strains in pot trials and field experiments, or both.
- Full Text:
- Date Issued: 2020
Towards the synthesis of trimethoprim using continuous flow Chemistry
- Authors: Ngwenya, Sandiso
- Date: 2020
- Subjects: Trimethoprim
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10948/47475 , vital:39994
- Description: The continuous flow synthesis of trimethoprim, an antibiotic, was investigated in this study from simple commercially available reagents, such as aniline and dimethylamine. The first step of the synthesis involves a highly exothermic Michael reaction, between acrylonitrile and dimethylamine; process intensification through miniaturization provides a safe and efficient method of synthesis through the use of microreactors. This is afforded by their large surface area-to-volume ratio; one of the many characteristics of continuous flow synthesis that allows for rapid heat dissipation allowing for exothermic reaction to be conducted safely at ambient or higher temperatures. This study was optimized on a Chemtrix Labtrix® Start unit using a Chemtrix reactor achieving a conversion of 99% in 12 sec residence time at 25oC. Subsequently the base catalyzed condensation of the intermediate with 3,4,5- trimethoxybenzaldehyde was investigated using Little Things Factory reactors (Channel diameter: 1.0mm). Maximum conversion (100%) was obtained at 80oC in 20 mins. Due to the poor cyclisation observed with this intermediate, according to literature findings, the 3-dimethylamino group was substituted for aniline. The optimisation for this acid catalysed substitution reaction was performed on a homemade PTFE tubing reactor (Diameter: 0.5mm) and it was found that at 120oC in 10 mins residence time a conversion of 97% was obtained. From the results obtained, an investigation into an integrated process for the condensation and substitution reaction was done using the Little Things Factory system (Channel Diameter: 1.0mm) coupled with a homemade PTFE coil reactor (Diameter: 0.5mm). This process, under optimum conditions of temperature 80oC and residence time of 30 mins, attained a conversion of 76%.
- Full Text:
- Date Issued: 2020
- Authors: Ngwenya, Sandiso
- Date: 2020
- Subjects: Trimethoprim
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10948/47475 , vital:39994
- Description: The continuous flow synthesis of trimethoprim, an antibiotic, was investigated in this study from simple commercially available reagents, such as aniline and dimethylamine. The first step of the synthesis involves a highly exothermic Michael reaction, between acrylonitrile and dimethylamine; process intensification through miniaturization provides a safe and efficient method of synthesis through the use of microreactors. This is afforded by their large surface area-to-volume ratio; one of the many characteristics of continuous flow synthesis that allows for rapid heat dissipation allowing for exothermic reaction to be conducted safely at ambient or higher temperatures. This study was optimized on a Chemtrix Labtrix® Start unit using a Chemtrix reactor achieving a conversion of 99% in 12 sec residence time at 25oC. Subsequently the base catalyzed condensation of the intermediate with 3,4,5- trimethoxybenzaldehyde was investigated using Little Things Factory reactors (Channel diameter: 1.0mm). Maximum conversion (100%) was obtained at 80oC in 20 mins. Due to the poor cyclisation observed with this intermediate, according to literature findings, the 3-dimethylamino group was substituted for aniline. The optimisation for this acid catalysed substitution reaction was performed on a homemade PTFE tubing reactor (Diameter: 0.5mm) and it was found that at 120oC in 10 mins residence time a conversion of 97% was obtained. From the results obtained, an investigation into an integrated process for the condensation and substitution reaction was done using the Little Things Factory system (Channel Diameter: 1.0mm) coupled with a homemade PTFE coil reactor (Diameter: 0.5mm). This process, under optimum conditions of temperature 80oC and residence time of 30 mins, attained a conversion of 76%.
- Full Text:
- Date Issued: 2020
Towards understanding how exploitation influences the wild energetic response of marine fish to temperature variability
- Authors: Skeeles, Michael Richard
- Date: 2020
- Subjects: Chrysoblepus laticeps -- Climatic factors , Sparidae -- Genetics , Sparidae -- South Africa -- Climatic factors
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/145133 , vital:38411
- Description: Exploitation of fish populations can exacerbate the effects of climate change, yet our understanding of their synergistic effects remains limited. As fish are increasingly exposed to temperatures on the edges of their optimal thermal performance window, their physiological response is expected to shape their future performance. It is therefore concerning that exploitation can select for specific physiological phenotypes, as this may affect fished populations’ physiological response to temperature change. A recent laboratory study revealed fewer high-performance metabolic-scope phenotypes in an exploited population of the marine Sparid Chrysoblepus laticeps across a range of experimental temperatures in comparison to an unexploited population. This suggested that individuals in exploited populations may have less available energy for aerobic performance at thermal extremes, which may reduce the resilience of the population to changes in temperature. However, since laboratory experiments exclude numerous other variables that fish encounter in the wild, it was necessary to test this finding in a natural setting. This thesis aimed to further develop the laboratory study by assessing whether exploitation effects the wild energetic response of C. laticeps to thermal variability. To achieve this, the field metabolic rate of C. laticeps, a resident and endemic South African fish, from a near-pristine population (Tsitsikamma National Park) and a heavily exploited population (Port Elizabeth) was compared using acoustic accelerometry. A laboratory-based study using a swim-tunnel respirometer and accelerometer transmitters was conducted to develop a model to predict metabolic rate from acceleration data at temperatures from 10 to 22⁰C. Acceleration, temperature, mass and population (exploited/unexploited) were found to be the best predictors of the metabolic rate of C. laticeps and were incorporated into the model to estimate the field metabolic rate of fish tagged with acoustic accelerometers in the wild. To examine the combined effects of temperature and exploitation on the field metabolic rate of C. laticeps in their natural state, two fine-scale telemetry arrays with temperature loggers were used to assess the acceleration of the fish across different temperatures in the wild for three months during a period of high thermal variability. Ten fish from the exploited and unexploited populations were caught, surgically implanted with accelerometer transmitters and released back into the wild. Close to 500 000 and 400 000 acceleration estimates were recorded from wild exploited and unexploited fish, respectively. The field metabolic rate of both populations was estimated by combining the field acceleration and temperature data with the laboratory calibration model. The field metabolic rate of C. laticeps from the exploited population was constrained near cold and warm extremes compared to no constraints observed in the unexploited population. This was attributed to reduced inter-individual variability in the field metabolic rate-temperature relationship within the exploited population. There appeared to be a greater proportion of individuals that maintained a high field metabolic rate at extreme temperatures in the unexploited population. In contrast, all but one fish from the exploited population did not maintain a high field metabolic rate at extreme temperatures. These findings aligned with the laboratory-based metabolic-scope study on both populations of C. laticeps and demonstrate that passive-fishing may be removing thermally tolerant individuals and rendering exploited populations less resilient to thermal change. These findings are discussed in the context of fisheries management and particularly on the role that marine protected areas could play in maintaining physiological diversity, and therefore the resilience of fish in the Anthropocene. This study highlights the importance of applied conservation physiology in understanding the consequences of fisheries-induced evolution in an increasingly variable climate.
- Full Text:
- Date Issued: 2020
- Authors: Skeeles, Michael Richard
- Date: 2020
- Subjects: Chrysoblepus laticeps -- Climatic factors , Sparidae -- Genetics , Sparidae -- South Africa -- Climatic factors
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/145133 , vital:38411
- Description: Exploitation of fish populations can exacerbate the effects of climate change, yet our understanding of their synergistic effects remains limited. As fish are increasingly exposed to temperatures on the edges of their optimal thermal performance window, their physiological response is expected to shape their future performance. It is therefore concerning that exploitation can select for specific physiological phenotypes, as this may affect fished populations’ physiological response to temperature change. A recent laboratory study revealed fewer high-performance metabolic-scope phenotypes in an exploited population of the marine Sparid Chrysoblepus laticeps across a range of experimental temperatures in comparison to an unexploited population. This suggested that individuals in exploited populations may have less available energy for aerobic performance at thermal extremes, which may reduce the resilience of the population to changes in temperature. However, since laboratory experiments exclude numerous other variables that fish encounter in the wild, it was necessary to test this finding in a natural setting. This thesis aimed to further develop the laboratory study by assessing whether exploitation effects the wild energetic response of C. laticeps to thermal variability. To achieve this, the field metabolic rate of C. laticeps, a resident and endemic South African fish, from a near-pristine population (Tsitsikamma National Park) and a heavily exploited population (Port Elizabeth) was compared using acoustic accelerometry. A laboratory-based study using a swim-tunnel respirometer and accelerometer transmitters was conducted to develop a model to predict metabolic rate from acceleration data at temperatures from 10 to 22⁰C. Acceleration, temperature, mass and population (exploited/unexploited) were found to be the best predictors of the metabolic rate of C. laticeps and were incorporated into the model to estimate the field metabolic rate of fish tagged with acoustic accelerometers in the wild. To examine the combined effects of temperature and exploitation on the field metabolic rate of C. laticeps in their natural state, two fine-scale telemetry arrays with temperature loggers were used to assess the acceleration of the fish across different temperatures in the wild for three months during a period of high thermal variability. Ten fish from the exploited and unexploited populations were caught, surgically implanted with accelerometer transmitters and released back into the wild. Close to 500 000 and 400 000 acceleration estimates were recorded from wild exploited and unexploited fish, respectively. The field metabolic rate of both populations was estimated by combining the field acceleration and temperature data with the laboratory calibration model. The field metabolic rate of C. laticeps from the exploited population was constrained near cold and warm extremes compared to no constraints observed in the unexploited population. This was attributed to reduced inter-individual variability in the field metabolic rate-temperature relationship within the exploited population. There appeared to be a greater proportion of individuals that maintained a high field metabolic rate at extreme temperatures in the unexploited population. In contrast, all but one fish from the exploited population did not maintain a high field metabolic rate at extreme temperatures. These findings aligned with the laboratory-based metabolic-scope study on both populations of C. laticeps and demonstrate that passive-fishing may be removing thermally tolerant individuals and rendering exploited populations less resilient to thermal change. These findings are discussed in the context of fisheries management and particularly on the role that marine protected areas could play in maintaining physiological diversity, and therefore the resilience of fish in the Anthropocene. This study highlights the importance of applied conservation physiology in understanding the consequences of fisheries-induced evolution in an increasingly variable climate.
- Full Text:
- Date Issued: 2020
Towards user experience principles for Electronic Tax in South Africa
- Authors: Mzili, Xolelwa
- Date: 2020
- Subjects: Electronic filing systems -- South Africa Tax returns -- South Africa
- Language: English
- Type: Thesis , Masters , MIT
- Identifier: http://hdl.handle.net/10948/49529 , vital:41733
- Description: Electronic Tax is a convenient system that enables taxpayers to submit their tax returns electronically, from home or from the office at any time of the day during the tax filing season. However, the electronic tax system has faced challenges over the years and to date. Thus, the reason for this study was to recommend user experience and usability principles for the electronic tax system to enhance the experience of taxpayers when using the electronic tax submission system. These principles contributed to improving the electronic tax system and increasing the adoption of the system among taxpayers who still submit their tax returns, manually. The study firstly described the problem, which forms part of the foundation for this research, namely development, which then led to the research questions and objectives, forming the core for the development of this study. User experience and usability principles are available for an online system, but there are limited studies on these factors regarding the electronic tax system. This study emphasized the importance of applying the user experience and usability principles to the electronic tax system. The various factors that affect the South African electronic tax system as a case study, have also been identified, including those that prompt other taxpayers to file at the SARS branch. As a result, to prove this theory, a questionnaire was sent only to electronic taxpayers to investigate how they perceive the electronic tax system. It can be concluded that the system has both advantages and disadvantages. An advantage is that the electronic tax system enables taxpayers to submit returns online from home or the office, instead of waiting in queues at the SARS branch. Disadvantages include that some taxpayers are concerned that their information is not safe and some encounter error messages and solutions while interacting with the system and these are not in a simple language for them to understand and recover. The user experience and usability principles have been recommended based on the responses obtained from the questionnaire.
- Full Text:
- Date Issued: 2020
- Authors: Mzili, Xolelwa
- Date: 2020
- Subjects: Electronic filing systems -- South Africa Tax returns -- South Africa
- Language: English
- Type: Thesis , Masters , MIT
- Identifier: http://hdl.handle.net/10948/49529 , vital:41733
- Description: Electronic Tax is a convenient system that enables taxpayers to submit their tax returns electronically, from home or from the office at any time of the day during the tax filing season. However, the electronic tax system has faced challenges over the years and to date. Thus, the reason for this study was to recommend user experience and usability principles for the electronic tax system to enhance the experience of taxpayers when using the electronic tax submission system. These principles contributed to improving the electronic tax system and increasing the adoption of the system among taxpayers who still submit their tax returns, manually. The study firstly described the problem, which forms part of the foundation for this research, namely development, which then led to the research questions and objectives, forming the core for the development of this study. User experience and usability principles are available for an online system, but there are limited studies on these factors regarding the electronic tax system. This study emphasized the importance of applying the user experience and usability principles to the electronic tax system. The various factors that affect the South African electronic tax system as a case study, have also been identified, including those that prompt other taxpayers to file at the SARS branch. As a result, to prove this theory, a questionnaire was sent only to electronic taxpayers to investigate how they perceive the electronic tax system. It can be concluded that the system has both advantages and disadvantages. An advantage is that the electronic tax system enables taxpayers to submit returns online from home or the office, instead of waiting in queues at the SARS branch. Disadvantages include that some taxpayers are concerned that their information is not safe and some encounter error messages and solutions while interacting with the system and these are not in a simple language for them to understand and recover. The user experience and usability principles have been recommended based on the responses obtained from the questionnaire.
- Full Text:
- Date Issued: 2020
Tracing the Voice of Protest in Selected Oral Literature:
- Authors: Kaschula, Russell H
- Date: 2020
- Language: English
- Type: text , book
- Identifier: http://hdl.handle.net/10962/174452 , vital:42479 , ISBN 9783030555177
- Description: This chapter aims to present the notion of “protest” as a contested arena in the production of oral literature, against the backdrop of continued and contested sociopolitical change in Africa. This contestation can be politically based, gender based and sometimes even based in religion. The chapter looks specifically at poetry, songs and the folktale to establish how the “voice of protest” has been presented over time in relation to specific African countries such as Senegal, South Africa, Somali, Guinea, Kenya, Tanzania, Malawi, Ethiopia, Nigeria, Zimbabwe and Lesotho. Poetic protest as part of political protest is also discussed. Furthermore, the chapter seeks to explore how this “voice of protest” has been received, both from an audience point of view and from the point of view of those who control power.
- Full Text:
- Date Issued: 2020
- Authors: Kaschula, Russell H
- Date: 2020
- Language: English
- Type: text , book
- Identifier: http://hdl.handle.net/10962/174452 , vital:42479 , ISBN 9783030555177
- Description: This chapter aims to present the notion of “protest” as a contested arena in the production of oral literature, against the backdrop of continued and contested sociopolitical change in Africa. This contestation can be politically based, gender based and sometimes even based in religion. The chapter looks specifically at poetry, songs and the folktale to establish how the “voice of protest” has been presented over time in relation to specific African countries such as Senegal, South Africa, Somali, Guinea, Kenya, Tanzania, Malawi, Ethiopia, Nigeria, Zimbabwe and Lesotho. Poetic protest as part of political protest is also discussed. Furthermore, the chapter seeks to explore how this “voice of protest” has been received, both from an audience point of view and from the point of view of those who control power.
- Full Text:
- Date Issued: 2020
Traditional Dealerships’ Operational Capacity to Sell Electric Vehicles
- Authors: Knoetze, Alicia Jo-mari
- Date: 2020
- Subjects: Electric vehicle industry Automobile industry and trade
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: http://hdl.handle.net/10948/48686 , vital:41059
- Description: The global automotive industry faces an emerging turning point where an unceasing dependence on costly fossil fuels and a growing concern over global warming is driving the industry to form new possibilities. A major challenge for decision makers is to assure that the consumer market is informed about both the advantages and disadvantageous of electric vehicle technology. A significant amount of research has been done on both the operational ability of electric vehicles and consumer resistance and preferences towards new technology and the marketing of electric vehicles. Given the retail relationship between key industry stakeholders such as the Original Equipment Manufacturers, franchised dealership network and potential electric vehicle consumers, the transition towards new electric vehicle technology represents an important strategic realignment for dealerships in order to sell electric vehicles along with traditional internal combustion engine vehicles and questions their operational capacity to do so successfully. The primary data was collected from a sample of forty respondents by means of an online questionnaire. The questionnaire was designed from literature and using similar extracts from other electric vehicle consumer resistance studies questionnaires. An email containing a Universal Resource Link (URL) to the questionnaire was sent and by means of snowball sampling, a representative sample of forty-eight respondents participated in the study who fully completed all the sections of the questionnaire. Interviews were conducted with Senior Management of local dealerships in the Port Elizabeth area. The interviews were executed in the same method as a ‘walk-in’ potential customer. Participation therefore was based on the willingness, readiness and availability of management. Three dealerships who already sell electric vehicles as well as two dealership who still only sell traditional internal combustion engine vehicles participated in the interviews. In addition, the National Automobile Dealers Association (NADA) commented on the strategic realignment of the South African industry as a whole. The results indicated that the majority of respondents believed that electric vehicles were a suitable alternative to the traditional internal combustion engine vehicle and were willing to personally contribute to improved sustainable mobility. Uncertainty remained with consumers and there was unwillingness to purchase and electric vehicle within the next five years. Electric vehicles were also believed to perform better but there was uncertainty about the return that would be achieved from investment in an electric vehicle despite the reduction in their carbon footprint and the associated image boost with family and colleagues. Furthermore, there appeared to be an alignment amongst the interviewees that South Africa does not face and immediate threat in terms of electric vehicles as the anticipated tipping point is envisaged to be in the region of 2030 onwards, however NADA as a professional industry body still warns that this is not a set point in time and that market forces could change the outlook and adaption dramatically.
- Full Text:
- Date Issued: 2020
- Authors: Knoetze, Alicia Jo-mari
- Date: 2020
- Subjects: Electric vehicle industry Automobile industry and trade
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: http://hdl.handle.net/10948/48686 , vital:41059
- Description: The global automotive industry faces an emerging turning point where an unceasing dependence on costly fossil fuels and a growing concern over global warming is driving the industry to form new possibilities. A major challenge for decision makers is to assure that the consumer market is informed about both the advantages and disadvantageous of electric vehicle technology. A significant amount of research has been done on both the operational ability of electric vehicles and consumer resistance and preferences towards new technology and the marketing of electric vehicles. Given the retail relationship between key industry stakeholders such as the Original Equipment Manufacturers, franchised dealership network and potential electric vehicle consumers, the transition towards new electric vehicle technology represents an important strategic realignment for dealerships in order to sell electric vehicles along with traditional internal combustion engine vehicles and questions their operational capacity to do so successfully. The primary data was collected from a sample of forty respondents by means of an online questionnaire. The questionnaire was designed from literature and using similar extracts from other electric vehicle consumer resistance studies questionnaires. An email containing a Universal Resource Link (URL) to the questionnaire was sent and by means of snowball sampling, a representative sample of forty-eight respondents participated in the study who fully completed all the sections of the questionnaire. Interviews were conducted with Senior Management of local dealerships in the Port Elizabeth area. The interviews were executed in the same method as a ‘walk-in’ potential customer. Participation therefore was based on the willingness, readiness and availability of management. Three dealerships who already sell electric vehicles as well as two dealership who still only sell traditional internal combustion engine vehicles participated in the interviews. In addition, the National Automobile Dealers Association (NADA) commented on the strategic realignment of the South African industry as a whole. The results indicated that the majority of respondents believed that electric vehicles were a suitable alternative to the traditional internal combustion engine vehicle and were willing to personally contribute to improved sustainable mobility. Uncertainty remained with consumers and there was unwillingness to purchase and electric vehicle within the next five years. Electric vehicles were also believed to perform better but there was uncertainty about the return that would be achieved from investment in an electric vehicle despite the reduction in their carbon footprint and the associated image boost with family and colleagues. Furthermore, there appeared to be an alignment amongst the interviewees that South Africa does not face and immediate threat in terms of electric vehicles as the anticipated tipping point is envisaged to be in the region of 2030 onwards, however NADA as a professional industry body still warns that this is not a set point in time and that market forces could change the outlook and adaption dramatically.
- Full Text:
- Date Issued: 2020
Transformative ICT education practices in rural secondary schools for developmental needs and realities: the Eastern Cape Province, South Africa
- Authors: Simuja, Clement
- Date: 2020
- Subjects: Education, Secondary -- South Africa -- Data processing , Information technology -- Study and teaching (Secondary) --South Africa , Educational technology -- Developing countries , Rural development -- Developing countries , Computer-assisted instruction -- South Africa -- Eastern Cape , Internet in education -- South Africa , Rural schools -- South Africa -- Eastern Cape , Community and school -- South Africa -- Eastern Cape
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/150631 , vital:38991
- Description: The perceived social development significance of Information and Communication Technology (ICT) has dramatically expanded the domains in which this cluster of ICTs is being discussed and acted upon. The action to promote community development in rural areas in South Africa has made its way into the introduction of ICT education in secondary schools. Since rural secondary schools form part of the framework for rural communities, they are being challenged to provide ICT education that makes a difference in learners’ lives. This requires engaging education practices that inspire learners to construct knowledge of ICT that does not only respond to examination purposes but rather, to the needs and development aspirations of the community. This research examines the experience of engaging learners and communities in socially informed ICT education in rural secondary schools. Specifically, it seeks to develop a critique of current practices involved in ICT education in rural secondary schools, and explores plausible alternatives to such practices that would make ICT education more transformative and structured towards the developmental concerns of communities. The main empirical focus for the research was five rural secondary schools in the Eastern Cape Province in South Africa. The research involved 53 participants that participated in a socially informed ICT training process. The training was designed to inspire participants to share their self-defined ICT education and ICT knowledge experiences. Critical Action Learning and Philosophical Inquiry provided the methodological framework, whilst the theoretical framework draws on Foucault’s philosophical ideas on power-knowledge relations. Through this theoretical analysis, the research examines the dynamic interplay of practices in ICT education with the values, ideals, and knowledge that form the core-life experiences of learners and rural communities. The research findings of this study indicate that current ICT education practices in rural secondary schools are endowed with ideologies that are affecting learners’ identity, social experiences, power, and ownership of the reflective meaning of using ICTs in community development. The contribution of this thesis lies in demonstrating ways that reframe ICT education transformatively, and more specifically its practices in the light of the way power, identity, ownership and social experience construct and offer learners a transformative view of self and the world. This could enable ICT education to fulfil the potential of contributing to social development in rural communities. The thesis culminates by presenting a theoretical framework that articulates the structural and authoritative components of ICT education practices – these relate to learners’ conscious understandings and represented thoughts, sensations and meanings embedded in the context, and actions and locations of using their knowledge of ICT.
- Full Text:
- Date Issued: 2020
- Authors: Simuja, Clement
- Date: 2020
- Subjects: Education, Secondary -- South Africa -- Data processing , Information technology -- Study and teaching (Secondary) --South Africa , Educational technology -- Developing countries , Rural development -- Developing countries , Computer-assisted instruction -- South Africa -- Eastern Cape , Internet in education -- South Africa , Rural schools -- South Africa -- Eastern Cape , Community and school -- South Africa -- Eastern Cape
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/150631 , vital:38991
- Description: The perceived social development significance of Information and Communication Technology (ICT) has dramatically expanded the domains in which this cluster of ICTs is being discussed and acted upon. The action to promote community development in rural areas in South Africa has made its way into the introduction of ICT education in secondary schools. Since rural secondary schools form part of the framework for rural communities, they are being challenged to provide ICT education that makes a difference in learners’ lives. This requires engaging education practices that inspire learners to construct knowledge of ICT that does not only respond to examination purposes but rather, to the needs and development aspirations of the community. This research examines the experience of engaging learners and communities in socially informed ICT education in rural secondary schools. Specifically, it seeks to develop a critique of current practices involved in ICT education in rural secondary schools, and explores plausible alternatives to such practices that would make ICT education more transformative and structured towards the developmental concerns of communities. The main empirical focus for the research was five rural secondary schools in the Eastern Cape Province in South Africa. The research involved 53 participants that participated in a socially informed ICT training process. The training was designed to inspire participants to share their self-defined ICT education and ICT knowledge experiences. Critical Action Learning and Philosophical Inquiry provided the methodological framework, whilst the theoretical framework draws on Foucault’s philosophical ideas on power-knowledge relations. Through this theoretical analysis, the research examines the dynamic interplay of practices in ICT education with the values, ideals, and knowledge that form the core-life experiences of learners and rural communities. The research findings of this study indicate that current ICT education practices in rural secondary schools are endowed with ideologies that are affecting learners’ identity, social experiences, power, and ownership of the reflective meaning of using ICTs in community development. The contribution of this thesis lies in demonstrating ways that reframe ICT education transformatively, and more specifically its practices in the light of the way power, identity, ownership and social experience construct and offer learners a transformative view of self and the world. This could enable ICT education to fulfil the potential of contributing to social development in rural communities. The thesis culminates by presenting a theoretical framework that articulates the structural and authoritative components of ICT education practices – these relate to learners’ conscious understandings and represented thoughts, sensations and meanings embedded in the context, and actions and locations of using their knowledge of ICT.
- Full Text:
- Date Issued: 2020
Transformative Power of Language Policies in Higher Education:
- Kaschula, Russell H, Docrat, Zakeera
- Authors: Kaschula, Russell H , Docrat, Zakeera
- Date: 2020
- Language: English
- Type: text , book
- Identifier: http://hdl.handle.net/10962/174502 , vital:42483 , ISBN 9781108498821
- Description: There is a disjuncture between the intentions of the South African constitutional provisions as contained in Section 6, which prescribe that all eleven official languages be treated equitably, and what is happening on the ground at the institutions of higher learning. In these institutions, English remains the primary language of instruction. African languages are encouraged as support languages in certain instances, but no institution has an African language as a medium of instruction (see for example the Rhodes Language Policy, 2019). This chapter calls into question the use of language and argues for purposive interpretation and practical implementation of the constitutional provisions on language. A proper and linguistically sound understanding of multilingualism as a developmental resource–educational, legal and societal–needs to emerge (Wolff, 2016). The constitutional aspects are explicated in the legislative and policy frameworks discussed in this chapter.
- Full Text:
- Date Issued: 2020
- Authors: Kaschula, Russell H , Docrat, Zakeera
- Date: 2020
- Language: English
- Type: text , book
- Identifier: http://hdl.handle.net/10962/174502 , vital:42483 , ISBN 9781108498821
- Description: There is a disjuncture between the intentions of the South African constitutional provisions as contained in Section 6, which prescribe that all eleven official languages be treated equitably, and what is happening on the ground at the institutions of higher learning. In these institutions, English remains the primary language of instruction. African languages are encouraged as support languages in certain instances, but no institution has an African language as a medium of instruction (see for example the Rhodes Language Policy, 2019). This chapter calls into question the use of language and argues for purposive interpretation and practical implementation of the constitutional provisions on language. A proper and linguistically sound understanding of multilingualism as a developmental resource–educational, legal and societal–needs to emerge (Wolff, 2016). The constitutional aspects are explicated in the legislative and policy frameworks discussed in this chapter.
- Full Text:
- Date Issued: 2020
Transforming environmental health practitioners’ knowledge-sharing practices through inter-agency formative intervention workshops
- Masilela, Priscilla, Olvitt, Lausanne L
- Authors: Masilela, Priscilla , Olvitt, Lausanne L
- Date: 2020
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/372809 , vital:66624 , xlink:href="https://doi.org/10.1080/0158037X.2020.1717458"
- Description: Home-based care facilities provide basic healthcare services to people too sick or frail to access formal clinics and hospitals. These facilities produce ‘healthcare risk waste’ which must be managed responsibly, and it is the work of Environmental Health Practitioners working within municipalities to ensure that the waste produced by home-based care facilities is managed in line with legislation. This paper presents a case study of a twenty-seven-month expansive learning intervention in a South African municipality that sought to transform its healthcare risk waste management practices. Limited knowledge and inadequate knowledge-sharing practices were identified as the main hindrances to effective waste management. The practitioner-researcher facilitated a series of inter-agency, formative intervention workshops with municipal employees and Community Health Workers using the Developmental Work Research methodology. These workshops strengthened both groups of practitioners’ knowledge of the ‘who, how, what, why and when’ that underpins effective healthcare risk waste management, and enabled ‘boundary crossing’ for practitioners to work across their specialist areas towards co-defining and analysing problems and constructing new solutions.
- Full Text:
- Date Issued: 2020
- Authors: Masilela, Priscilla , Olvitt, Lausanne L
- Date: 2020
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/372809 , vital:66624 , xlink:href="https://doi.org/10.1080/0158037X.2020.1717458"
- Description: Home-based care facilities provide basic healthcare services to people too sick or frail to access formal clinics and hospitals. These facilities produce ‘healthcare risk waste’ which must be managed responsibly, and it is the work of Environmental Health Practitioners working within municipalities to ensure that the waste produced by home-based care facilities is managed in line with legislation. This paper presents a case study of a twenty-seven-month expansive learning intervention in a South African municipality that sought to transform its healthcare risk waste management practices. Limited knowledge and inadequate knowledge-sharing practices were identified as the main hindrances to effective waste management. The practitioner-researcher facilitated a series of inter-agency, formative intervention workshops with municipal employees and Community Health Workers using the Developmental Work Research methodology. These workshops strengthened both groups of practitioners’ knowledge of the ‘who, how, what, why and when’ that underpins effective healthcare risk waste management, and enabled ‘boundary crossing’ for practitioners to work across their specialist areas towards co-defining and analysing problems and constructing new solutions.
- Full Text:
- Date Issued: 2020
Transitions from rural contexts to and through higher education in South Africa: negotiating misrecognition
- Mgqwashu, Emmanuel M, Timmis, Sue, de Wet, Thea, Madondo, Nkosinathi Emmanuel
- Authors: Mgqwashu, Emmanuel M , Timmis, Sue , de Wet, Thea , Madondo, Nkosinathi Emmanuel
- Date: 2020
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/148435 , vital:38739 , DOI:10.1080/03057925.2020.1763165
- Description: This paper reports on an international collaborative study that investigated how students from rural contexts negotiate the transition to university, and how prior cultural and educational experiences influence their higher education trajectories. A qualitative, participatory methodology was adopted, centred on co-researcher narratives, digital artefacts and discussions. Findings demonstrate how family and community, including religious, study, and self-help groups, influenced their transitions into higher education and journey through university and to their identities, agency and sense of belonging. The paper argues that university practices, values and norms need to acknowledge and incorporate all students’ prior experiences and histories and recognise their powerful contribution in working towards a decolonial higher education.
- Full Text:
- Date Issued: 2020
- Authors: Mgqwashu, Emmanuel M , Timmis, Sue , de Wet, Thea , Madondo, Nkosinathi Emmanuel
- Date: 2020
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/148435 , vital:38739 , DOI:10.1080/03057925.2020.1763165
- Description: This paper reports on an international collaborative study that investigated how students from rural contexts negotiate the transition to university, and how prior cultural and educational experiences influence their higher education trajectories. A qualitative, participatory methodology was adopted, centred on co-researcher narratives, digital artefacts and discussions. Findings demonstrate how family and community, including religious, study, and self-help groups, influenced their transitions into higher education and journey through university and to their identities, agency and sense of belonging. The paper argues that university practices, values and norms need to acknowledge and incorporate all students’ prior experiences and histories and recognise their powerful contribution in working towards a decolonial higher education.
- Full Text:
- Date Issued: 2020
Transnational Crime in Deon Meyer’s Devil’s Peak and Santiago Gamboa’s Night Prayers:
- Authors: Naidu, Samantha
- Date: 2020
- Language: English
- Type: text , book
- Identifier: http://hdl.handle.net/10962/163881 , vital:41077 , ISBN 9783030534134 , https://0-doi.org.wam.seals.ac.za/10.1007/978-3-030-53413-4_2
- Description: Naidu argues that transnational crime wreaks havoc on global, national and personal levels in the postcolonial crime novels Devil’s Peak (2007) by South African author Deon Meyer and Night Prayers (2016) by Colombian author Santiago Gamboa. As postcolonial crime novels, they critique sociopolitical instability and corruption harking back to colonial times. Using mobility studies, Naidu interrogates the novels’ rendering of complex relations between the local and the global, and the past and the present. Despite stylistic and generic differences, both novels engage with the pervasive, transnational nature of criminal syndicates and current crimes which are a result of turbulent and unjust histories. Naidu examines the mobility of hapless victims, postcolonial anti-detectives and subversive heroines and comments on the ironic hope afforded by such figures.
- Full Text:
- Date Issued: 2020
- Authors: Naidu, Samantha
- Date: 2020
- Language: English
- Type: text , book
- Identifier: http://hdl.handle.net/10962/163881 , vital:41077 , ISBN 9783030534134 , https://0-doi.org.wam.seals.ac.za/10.1007/978-3-030-53413-4_2
- Description: Naidu argues that transnational crime wreaks havoc on global, national and personal levels in the postcolonial crime novels Devil’s Peak (2007) by South African author Deon Meyer and Night Prayers (2016) by Colombian author Santiago Gamboa. As postcolonial crime novels, they critique sociopolitical instability and corruption harking back to colonial times. Using mobility studies, Naidu interrogates the novels’ rendering of complex relations between the local and the global, and the past and the present. Despite stylistic and generic differences, both novels engage with the pervasive, transnational nature of criminal syndicates and current crimes which are a result of turbulent and unjust histories. Naidu examines the mobility of hapless victims, postcolonial anti-detectives and subversive heroines and comments on the ironic hope afforded by such figures.
- Full Text:
- Date Issued: 2020
Two new Caenis Species (Insecta: Ephemeroptera: Caenidae) from the Kruger National Park, South Africa:
- Malzacher, P, Barber-James, Helen M
- Authors: Malzacher, P , Barber-James, Helen M
- Date: 2020
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/150256 , vital:38954 , https://0-doi.org.wam.seals.ac.za/10.4001/003.028.0062
- Description: The new mayfly species Caenis albicans sp. n. and Caenis letabanensis sp. n. (Ephemeroptera: Caenidae) from the Kruger National Park, South Africa, are described herein. The new species were collected in the area of the confluence of the Olifants and Letaba Rivers. They belong to the Caenis – TPA group, a group widely distributed in Africa, characterised inter alia by forceps apically having a tuft of long spines. The material examined also contained samples from the Crocodile and Sabie Rivers, with larvae and imagines of Caenis brevipes Kimmins, 1956. The previously unknown larva of this species is also described.
- Full Text:
- Date Issued: 2020
- Authors: Malzacher, P , Barber-James, Helen M
- Date: 2020
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/150256 , vital:38954 , https://0-doi.org.wam.seals.ac.za/10.4001/003.028.0062
- Description: The new mayfly species Caenis albicans sp. n. and Caenis letabanensis sp. n. (Ephemeroptera: Caenidae) from the Kruger National Park, South Africa, are described herein. The new species were collected in the area of the confluence of the Olifants and Letaba Rivers. They belong to the Caenis – TPA group, a group widely distributed in Africa, characterised inter alia by forceps apically having a tuft of long spines. The material examined also contained samples from the Crocodile and Sabie Rivers, with larvae and imagines of Caenis brevipes Kimmins, 1956. The previously unknown larva of this species is also described.
- Full Text:
- Date Issued: 2020
Uhlalutyo lwenoveli kamahala ethi: yakhal’ indoda (2010).
- Authors: Nyiki, Phumelela Sibahle
- Date: 2020
- Subjects: Literature
- Language: English
- Type: Thesis , Masters , MA (African Languages)
- Identifier: http://hdl.handle.net/10353/18257 , vital:42246
- Description: The aim and scope of this study is to analyse the novel Yakhal’ Indoda (2010) written by Siphiwo Mahala. Since this book was written in the period known as the modern times in the new South Africa, it therefore tends to focus on the issues that disturb the peace of the citizens of South Africa exactly in this period of democratic South Africa. For that reason, the objective of this study is to associate the factors which Mahala has written about with those that are found in the contemporary South Africa which are regarded as issues disturbing the lives of South Africans at present. As it is of importance before the researcher deals with the central issue, there has to be a thorough knowledge of the theory of the subject that is being discussed at hand. The Literature Review with regard to this study takes into account the books written by Satyo, Duka, Brooks and Warren, to mention just a few. These authors, from their different academic perspectives, do their best on explaining the theory of the issues that will be discussed in this study. The Theoretical Framework that will be used in this study consists of the views of the writers who fought for a better political dispensation in the countries that were previously colonized. In this regard, the views of Ngùgì wa Thiong’o, and those of Steve Biko will be assessed in this study. The method that will be used in conducting this research is none other than that of Text Analysis. This simply means the researching student will focus mainly on the books found at the UFH library and other libraries. The chapter titles and their relevant outlines follow below: Chapter One is an introduction for this entire research. This chapter focuses more on the aims of this study, the importance or significance, limitations, methodology to be followed. Furthermore, this chapter gives a brief background of the author of the novel that will be analysed as well as the background of the novel. Chapter Two focuses on the theory of this research. This will be done through two theories. The researcher will first present a theory of the novel, as well as the Postcolonial theory. Under the novel theory, the researcher will discuss some vii features of the novel. However, the researcher’s emphasis will be on the theme that underlies in Mahala’s novel, Yakhal’ Indoda (2010). Chapter Three focuses on the theme of this research. The researcher will analyse the way Mahala portrays crime, corruption, unemployment and poverty in his novel. The researcher then will validate Mahala’s point of view against other authors with regards to the identified problems. Chapter Four focuses on the problems found in the Department of Education as well as rights of women as depicted in Mahala’s novel, Yakhal’ Indoda (2010). This will be accomplished through validation of the experienced and witnessed events in the new South Africa. Chapter Five focuses on the factors and events that are commonly associated with the Departments of Health and in Local Government, which are life threatening to society. This will be done by the researcher, pointing out some problems that Mahala in his book Yakhal’ Indoda (2010) attributes to the mentioned departments. Chapter Six is the general conclusion and exegesis on the study. This chapter gives a brief summary of the previous chapters, the findings regarding this study.
- Full Text:
- Date Issued: 2020
- Authors: Nyiki, Phumelela Sibahle
- Date: 2020
- Subjects: Literature
- Language: English
- Type: Thesis , Masters , MA (African Languages)
- Identifier: http://hdl.handle.net/10353/18257 , vital:42246
- Description: The aim and scope of this study is to analyse the novel Yakhal’ Indoda (2010) written by Siphiwo Mahala. Since this book was written in the period known as the modern times in the new South Africa, it therefore tends to focus on the issues that disturb the peace of the citizens of South Africa exactly in this period of democratic South Africa. For that reason, the objective of this study is to associate the factors which Mahala has written about with those that are found in the contemporary South Africa which are regarded as issues disturbing the lives of South Africans at present. As it is of importance before the researcher deals with the central issue, there has to be a thorough knowledge of the theory of the subject that is being discussed at hand. The Literature Review with regard to this study takes into account the books written by Satyo, Duka, Brooks and Warren, to mention just a few. These authors, from their different academic perspectives, do their best on explaining the theory of the issues that will be discussed in this study. The Theoretical Framework that will be used in this study consists of the views of the writers who fought for a better political dispensation in the countries that were previously colonized. In this regard, the views of Ngùgì wa Thiong’o, and those of Steve Biko will be assessed in this study. The method that will be used in conducting this research is none other than that of Text Analysis. This simply means the researching student will focus mainly on the books found at the UFH library and other libraries. The chapter titles and their relevant outlines follow below: Chapter One is an introduction for this entire research. This chapter focuses more on the aims of this study, the importance or significance, limitations, methodology to be followed. Furthermore, this chapter gives a brief background of the author of the novel that will be analysed as well as the background of the novel. Chapter Two focuses on the theory of this research. This will be done through two theories. The researcher will first present a theory of the novel, as well as the Postcolonial theory. Under the novel theory, the researcher will discuss some vii features of the novel. However, the researcher’s emphasis will be on the theme that underlies in Mahala’s novel, Yakhal’ Indoda (2010). Chapter Three focuses on the theme of this research. The researcher will analyse the way Mahala portrays crime, corruption, unemployment and poverty in his novel. The researcher then will validate Mahala’s point of view against other authors with regards to the identified problems. Chapter Four focuses on the problems found in the Department of Education as well as rights of women as depicted in Mahala’s novel, Yakhal’ Indoda (2010). This will be accomplished through validation of the experienced and witnessed events in the new South Africa. Chapter Five focuses on the factors and events that are commonly associated with the Departments of Health and in Local Government, which are life threatening to society. This will be done by the researcher, pointing out some problems that Mahala in his book Yakhal’ Indoda (2010) attributes to the mentioned departments. Chapter Six is the general conclusion and exegesis on the study. This chapter gives a brief summary of the previous chapters, the findings regarding this study.
- Full Text:
- Date Issued: 2020
Ukuchichimala kwechweba
- Authors: Mbungwana, Mthunzikzi
- Date: 2020
- Subjects: Xhosa poetry
- Language: Xhosa
- Type: text , Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10962/168512 , vital:41590
- Description: iThisisi nepotifoliyo. It is a collection of poems relating to women and society and gender related issues including gender based violence. It also focuses on how Christianity and Xhosa culture has violated women's rights and how they both believe that men and women should have different roles in society.
- Full Text:
- Date Issued: 2020
- Authors: Mbungwana, Mthunzikzi
- Date: 2020
- Subjects: Xhosa poetry
- Language: Xhosa
- Type: text , Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10962/168512 , vital:41590
- Description: iThisisi nepotifoliyo. It is a collection of poems relating to women and society and gender related issues including gender based violence. It also focuses on how Christianity and Xhosa culture has violated women's rights and how they both believe that men and women should have different roles in society.
- Full Text:
- Date Issued: 2020