Bitten
- Authors: Sullivan, Louella
- Date: 2015
- Subjects: Creative writing (Higher education) , South African poetry (English) -- Study and teaching (Higher) , South African poerty (English) -- 21st century
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:5994 , http://hdl.handle.net/10962/d1017778
- Description: My poetry investigates the extraordinary in the everyday, exploring my life as a mother and wife, to find the quiet truths that lie there. Using fresh ways of describing familiar experiences, the poems describe tiny, almost-missed moments and voices that have shaped me. Throughout the collection, I imagine my younger selves commenting on my current self and vice versa. Ultimately, my poems use simple words and clean lines to evoke how I feel (and how I want the reader to feel) in each of the moments they describe.
- Full Text:
- Date Issued: 2015
- Authors: Sullivan, Louella
- Date: 2015
- Subjects: Creative writing (Higher education) , South African poetry (English) -- Study and teaching (Higher) , South African poerty (English) -- 21st century
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:5994 , http://hdl.handle.net/10962/d1017778
- Description: My poetry investigates the extraordinary in the everyday, exploring my life as a mother and wife, to find the quiet truths that lie there. Using fresh ways of describing familiar experiences, the poems describe tiny, almost-missed moments and voices that have shaped me. Throughout the collection, I imagine my younger selves commenting on my current self and vice versa. Ultimately, my poems use simple words and clean lines to evoke how I feel (and how I want the reader to feel) in each of the moments they describe.
- Full Text:
- Date Issued: 2015
Britain after the Romans : an interdisciplinary approach to the possibilities of an Adventus Saxonum
- Lloyd-Jones, Glyn Francis Michael
- Authors: Lloyd-Jones, Glyn Francis Michael
- Date: 2015
- Subjects: Great Britain -- History -- Anglo-Saxon period, 449-1066 , Civilization, Anglo-Saxon , English philology -- Old English, ca. 450-1100 , English literature -- Old English, ca. 450-1100 , Anglo-Saxon race , Genetic genealogy
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:3657 , http://hdl.handle.net/10962/d1019806
- Description: In the fifth century, after the departure of the Romans, according to tradition, which is based on the ancient written sources, Britain was invaded by the Angles and Saxons. This view has been questioned in the last century. The size of the ‘invasion’, and indeed its very existence, have come into doubt. However, this doubting school of thought does not seem to take into account all of the evidence. An interdisciplinary, nuanced approach has been taken in this thesis. Firstly, the question of Germanic raiding has been examined, with reference to the Saxon Shore defences. It is argued that these defences, in their geographical context, point to the likelihood of raiding. Then the written sources have been re-examined, as well as physical artefacts. In addition to geography, literature and archaeology (the disciplines which are most commonly used when the coming of the Angles and Saxons is investigated), linguistic and genetic data have been examined. The fields of linguistics and genetics, which have not often both been taken into consideration with previous approaches, add a number of valuable insights. This nuanced approach yields a picture of events that rules out the ‘traditional view’ in some ways, such as the idea that the Saxons exterminated the Britons altogether, but corroborates it in other ways. There was an invasion of a kind (of Angles – not Saxons), who came in comparatively small numbers, but found in Britain a society already mixed and comprising Celtic and Germanic-speaking peoples: a society implied by Caesar and Tacitus and corroborated by linguistic and genetic data.
- Full Text:
- Date Issued: 2015
Britain after the Romans : an interdisciplinary approach to the possibilities of an Adventus Saxonum
- Authors: Lloyd-Jones, Glyn Francis Michael
- Date: 2015
- Subjects: Great Britain -- History -- Anglo-Saxon period, 449-1066 , Civilization, Anglo-Saxon , English philology -- Old English, ca. 450-1100 , English literature -- Old English, ca. 450-1100 , Anglo-Saxon race , Genetic genealogy
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:3657 , http://hdl.handle.net/10962/d1019806
- Description: In the fifth century, after the departure of the Romans, according to tradition, which is based on the ancient written sources, Britain was invaded by the Angles and Saxons. This view has been questioned in the last century. The size of the ‘invasion’, and indeed its very existence, have come into doubt. However, this doubting school of thought does not seem to take into account all of the evidence. An interdisciplinary, nuanced approach has been taken in this thesis. Firstly, the question of Germanic raiding has been examined, with reference to the Saxon Shore defences. It is argued that these defences, in their geographical context, point to the likelihood of raiding. Then the written sources have been re-examined, as well as physical artefacts. In addition to geography, literature and archaeology (the disciplines which are most commonly used when the coming of the Angles and Saxons is investigated), linguistic and genetic data have been examined. The fields of linguistics and genetics, which have not often both been taken into consideration with previous approaches, add a number of valuable insights. This nuanced approach yields a picture of events that rules out the ‘traditional view’ in some ways, such as the idea that the Saxons exterminated the Britons altogether, but corroborates it in other ways. There was an invasion of a kind (of Angles – not Saxons), who came in comparatively small numbers, but found in Britain a society already mixed and comprising Celtic and Germanic-speaking peoples: a society implied by Caesar and Tacitus and corroborated by linguistic and genetic data.
- Full Text:
- Date Issued: 2015
Building an E-health system for health awareness campaigns in poor areas
- Authors: Gremu, Chikumbutso David
- Date: 2015
- Subjects: National health services -- South Africa , Medical informatics , Public health -- Information services
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4708 , http://hdl.handle.net/10962/d1017930
- Description: Appropriate e-services as well as revenue generation capabilities are key to the deployment and the sustainability for ICT installations in poor areas, particularly common in developing country. The area of e-Health is a promising area for e-services that are both important to the population in those areas and potentially of direct interest to National Health Organizations, which already spend money for Health campaigns there. This thesis focuses on the design, implementation, and full functional testing of HealthAware, an application that allows health organization to set up targeted awareness campaigns for poor areas. Requirements for such application are very specific, starting from the fact that the preparation of the campaign and its execution/consumption happen in two different environments from a technological and social point of view. Part of the research work done for this thesis was to make the above requirements explicit and then use them in the design. This phase of the research was facilitated by the fact that the thesis' work was executed within the context of the Siyakhula Living Lab (SLL; www.siyakhulaLL.org), which has accumulated multi-year experience of ICT deployment in such areas. As a result of the found requirements, HealthAware comprises two components, which are web-based, Java applications that run in a peer-to-peer fashion. The first component, the Dashboard, is used to create, manage, and publish information for conducting awareness campaigns or surveys. The second component, HealthMessenger, facilitates users' access to the campaigns or surveys that were created using the Dashboard. The HealthMessenger was designed to be hosted on TeleWeaver while the Dashboard is hosted independently of TeleWeaver and simply communicates with the HealthMessenger through webservices. TeleWeaver is an application integration platform developed within the SLL to host software applications for poor areas. Using a core service of TeleWeaver, the profile service, where all the users' defining elements are contained, campaigns and surveys can be easily and effectively targeted, for example to match specific demographics or geographic locations. Revenue generation is attained via the logging of the interactions of the target users in the communities with the applications in TeleWeaver, from which billing data is generated according to the specific contractual agreements with the National Health Organization. From a general point of view, HealthAware contributes to the concrete realizations of a bidirectional access channel between Health Organizations and users in poor communities, which not only allows the communication of appropriate content in both directions, but get 'monetized' and in so doing becomes a revenue generator.
- Full Text:
- Date Issued: 2015
- Authors: Gremu, Chikumbutso David
- Date: 2015
- Subjects: National health services -- South Africa , Medical informatics , Public health -- Information services
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4708 , http://hdl.handle.net/10962/d1017930
- Description: Appropriate e-services as well as revenue generation capabilities are key to the deployment and the sustainability for ICT installations in poor areas, particularly common in developing country. The area of e-Health is a promising area for e-services that are both important to the population in those areas and potentially of direct interest to National Health Organizations, which already spend money for Health campaigns there. This thesis focuses on the design, implementation, and full functional testing of HealthAware, an application that allows health organization to set up targeted awareness campaigns for poor areas. Requirements for such application are very specific, starting from the fact that the preparation of the campaign and its execution/consumption happen in two different environments from a technological and social point of view. Part of the research work done for this thesis was to make the above requirements explicit and then use them in the design. This phase of the research was facilitated by the fact that the thesis' work was executed within the context of the Siyakhula Living Lab (SLL; www.siyakhulaLL.org), which has accumulated multi-year experience of ICT deployment in such areas. As a result of the found requirements, HealthAware comprises two components, which are web-based, Java applications that run in a peer-to-peer fashion. The first component, the Dashboard, is used to create, manage, and publish information for conducting awareness campaigns or surveys. The second component, HealthMessenger, facilitates users' access to the campaigns or surveys that were created using the Dashboard. The HealthMessenger was designed to be hosted on TeleWeaver while the Dashboard is hosted independently of TeleWeaver and simply communicates with the HealthMessenger through webservices. TeleWeaver is an application integration platform developed within the SLL to host software applications for poor areas. Using a core service of TeleWeaver, the profile service, where all the users' defining elements are contained, campaigns and surveys can be easily and effectively targeted, for example to match specific demographics or geographic locations. Revenue generation is attained via the logging of the interactions of the target users in the communities with the applications in TeleWeaver, from which billing data is generated according to the specific contractual agreements with the National Health Organization. From a general point of view, HealthAware contributes to the concrete realizations of a bidirectional access channel between Health Organizations and users in poor communities, which not only allows the communication of appropriate content in both directions, but get 'monetized' and in so doing becomes a revenue generator.
- Full Text:
- Date Issued: 2015
Bush encroachment in the semi-arid communal grazing lands of Eastern Cape and farmer's perception of causes and livelihood impacts
- Authors: Libala, Notiswa
- Date: 2015
- Subjects: Communal rangelands--South Africa--Eastern Cape Grazing--South Africa--Eastern Cape egetation management--South Africa--Eastern Cape
- Language: English
- Type: Thesis , Masters , Pasture Science
- Identifier: http://hdl.handle.net/10353/11817 , vital:39109
- Description: The study was conducted in two communal rangelands of the Eastern Cape Province (Thorn and Mixed bush veld). The objective of the study was to investigate 1) the historical trends of bush encroachment, causes and impacts based on indigenous knowledge and perceptions of the communal people in two savannah vegetation types and 2) to determine the density and cover of woody species in the grazing areas. In each veld type, two villages were selected purposefully. A total of 120 households who own livestock were randomly selected from the four communal areas and a total of 48 elders were selected to provide information on bush encroachment and rangeland condition. For woody density and cover, grazing areas surrounding two homesteads (settlements) were selected from each communal rangeland, Two long transects radiating in the opposite direction from each homestead were established. The woody vegetation survey was conducted at 100, 200, 300, 400, 500, 600, 700, 800 and 900m distance from each homestead. In each distance, 3 (10 m x 10 m) quadrates were laid to record individual woody plant species, the height, basal stem and canopy diameters. Woody species were classified into seedlings (>0 - 1 m), saplings (>1 - 1.5 m), young shrubs (>1.5 - 2.0 m), mature shrubs (>2 - 3.0 m) and mature trees (>3.0 m). The average household size in of goat was higher than that of other livestock species. A total of 40 woody plant species were identified in all study areas. Acacia karoo, Coddia rudis and Scutia myrtina were the most dominant woody species in all study areas. Most of the woody plant species had the highest abundance in the height class >0 - 1 m. The average total woody plant density in Thorn bush and Mixed bush were 3461 and 2416 number ha-1, respectively. In conclusion both study areas were highly encroached and it was also perceived by elder from both areas that bush encroachment is a problem in the rangelands.
- Full Text:
- Date Issued: 2015
- Authors: Libala, Notiswa
- Date: 2015
- Subjects: Communal rangelands--South Africa--Eastern Cape Grazing--South Africa--Eastern Cape egetation management--South Africa--Eastern Cape
- Language: English
- Type: Thesis , Masters , Pasture Science
- Identifier: http://hdl.handle.net/10353/11817 , vital:39109
- Description: The study was conducted in two communal rangelands of the Eastern Cape Province (Thorn and Mixed bush veld). The objective of the study was to investigate 1) the historical trends of bush encroachment, causes and impacts based on indigenous knowledge and perceptions of the communal people in two savannah vegetation types and 2) to determine the density and cover of woody species in the grazing areas. In each veld type, two villages were selected purposefully. A total of 120 households who own livestock were randomly selected from the four communal areas and a total of 48 elders were selected to provide information on bush encroachment and rangeland condition. For woody density and cover, grazing areas surrounding two homesteads (settlements) were selected from each communal rangeland, Two long transects radiating in the opposite direction from each homestead were established. The woody vegetation survey was conducted at 100, 200, 300, 400, 500, 600, 700, 800 and 900m distance from each homestead. In each distance, 3 (10 m x 10 m) quadrates were laid to record individual woody plant species, the height, basal stem and canopy diameters. Woody species were classified into seedlings (>0 - 1 m), saplings (>1 - 1.5 m), young shrubs (>1.5 - 2.0 m), mature shrubs (>2 - 3.0 m) and mature trees (>3.0 m). The average household size in of goat was higher than that of other livestock species. A total of 40 woody plant species were identified in all study areas. Acacia karoo, Coddia rudis and Scutia myrtina were the most dominant woody species in all study areas. Most of the woody plant species had the highest abundance in the height class >0 - 1 m. The average total woody plant density in Thorn bush and Mixed bush were 3461 and 2416 number ha-1, respectively. In conclusion both study areas were highly encroached and it was also perceived by elder from both areas that bush encroachment is a problem in the rangelands.
- Full Text:
- Date Issued: 2015
Business events for the citizens of the Nelson Mandela Metropolitan Bay
- Authors: Hastie, Dean
- Date: 2015
- Subjects: Place marketing -- South Africa -- Port Elizabeth. , Sightseeing business -- South Africa -- Port Elizabeth
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: http://hdl.handle.net/10948/7554 , vital:21810
- Description: The effective application of destination marketing strategies leads to increased business activity within a city. This in turn leads to higher attainment levels of social and economic development. It can therefore be said that destination marketing strategies are an essential factor in the growth and functioning of a city. The objective of destination marketing is the city’s image or identity which in turn is the starting point for developing the city’s brand. Cities require an identity. It is crucial that a city has a unique identity with which visitors, tourists and residents can relate to. Examples of these unique city identities are Los Angeles which is known as the City of Angels and Florence which is known as the City of Lilies. The image of a place influences people’s perceptions and choices when visiting, living and conducting business in a specific city. The city’s image and brand serves as a conduit for city residents to identify with their city. The essence of the brand must be discovered, identified, brought to the surface and not imagined, created or added from the outside through an artificial process. Cities that make use of effective destination marketing strategies have endured a considerable increase in business and leisure activities. Notably, business events are important attractions and drivers for activity in many destinations. Multiple parties designing a brand strategy for a city should thoroughly assess the resources and assets a city possesses. A city branding strategy can enable a city to leverage its ‘tangible and non-tangible assets‟. In order for business tourism to develop in South Africa, it is essential that players within the sector have a thorough understanding of international dynamics in order to capitalise on the opportunities that are provided in South Africa. Nelson Mandela Bay in South Africa is a city with vast potential. The city is ideal as a tourist destination as it is situated along the Sunshine coast of South Africa. It could be said of the city that it is on the threshold of rising eminence in the coming decades. This treatise investigates the approach and success factors for a successful rebranding of Nelson Mandela Bay through business events as part of a destination marketing strategy. The treatise will ascertain which business events residents of the Nelson Mandela Bay would require and support. A survey was conducted among citizens of Nelson Mandela Bay using a questionnaire from which 952 responses were received. The questionnaire measured the respondents’ attitudes to which types of business events they would require and support their feelings on the brand identity of the city as well as the communication medium they would like to receive communication through. The findings of this study indicate that residents of Nelson Mandela Bay are open to the use of business events as the main driver in terms of business event attendance and support. Furthermore, the residents have made it clear they will support exhibitions, conventions and tradeshows. Facebook and local radio are identified as being the preferred communication method for hearing about events.
- Full Text:
- Date Issued: 2015
- Authors: Hastie, Dean
- Date: 2015
- Subjects: Place marketing -- South Africa -- Port Elizabeth. , Sightseeing business -- South Africa -- Port Elizabeth
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: http://hdl.handle.net/10948/7554 , vital:21810
- Description: The effective application of destination marketing strategies leads to increased business activity within a city. This in turn leads to higher attainment levels of social and economic development. It can therefore be said that destination marketing strategies are an essential factor in the growth and functioning of a city. The objective of destination marketing is the city’s image or identity which in turn is the starting point for developing the city’s brand. Cities require an identity. It is crucial that a city has a unique identity with which visitors, tourists and residents can relate to. Examples of these unique city identities are Los Angeles which is known as the City of Angels and Florence which is known as the City of Lilies. The image of a place influences people’s perceptions and choices when visiting, living and conducting business in a specific city. The city’s image and brand serves as a conduit for city residents to identify with their city. The essence of the brand must be discovered, identified, brought to the surface and not imagined, created or added from the outside through an artificial process. Cities that make use of effective destination marketing strategies have endured a considerable increase in business and leisure activities. Notably, business events are important attractions and drivers for activity in many destinations. Multiple parties designing a brand strategy for a city should thoroughly assess the resources and assets a city possesses. A city branding strategy can enable a city to leverage its ‘tangible and non-tangible assets‟. In order for business tourism to develop in South Africa, it is essential that players within the sector have a thorough understanding of international dynamics in order to capitalise on the opportunities that are provided in South Africa. Nelson Mandela Bay in South Africa is a city with vast potential. The city is ideal as a tourist destination as it is situated along the Sunshine coast of South Africa. It could be said of the city that it is on the threshold of rising eminence in the coming decades. This treatise investigates the approach and success factors for a successful rebranding of Nelson Mandela Bay through business events as part of a destination marketing strategy. The treatise will ascertain which business events residents of the Nelson Mandela Bay would require and support. A survey was conducted among citizens of Nelson Mandela Bay using a questionnaire from which 952 responses were received. The questionnaire measured the respondents’ attitudes to which types of business events they would require and support their feelings on the brand identity of the city as well as the communication medium they would like to receive communication through. The findings of this study indicate that residents of Nelson Mandela Bay are open to the use of business events as the main driver in terms of business event attendance and support. Furthermore, the residents have made it clear they will support exhibitions, conventions and tradeshows. Facebook and local radio are identified as being the preferred communication method for hearing about events.
- Full Text:
- Date Issued: 2015
Capacity building for developmental local government in the Kicukiro District of Rwanda
- Authors: Rutebuka, Balinda
- Date: 2015
- Subjects: Sustainable development -- Rwanda , Economic development -- Rwanda , Local government -- Rwanda
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: vital:8490 , http://hdl.handle.net/10948/d1020906
- Description: This study investigated the capacity building for developmental local government with reference to the Kicukiro District of Rwanda. Its aim was to examine the contribution of capacity-building interventions towards a developmental local government and at the same time investigate factors that hinder their implementation in Kicukiro district. The study provides an extensive historical background on local government in Rwa-nda with a particular focus on its policy, legal and institutional framework, whereby the developmental local government environment has been analysed. Furthermore, this study explores the theoretical framework of capacity building in general, and in this regard particular emphasis was given to capacity building in relation to developmental local government. The study also argued that without appropriate capacity it would be difficult for the district to fulfil its developmental mandate. This research followed both qualitative and quantitative approaches of study. It involved a survey in which a structured questionnaire and in-depth interviews were used as data gathering instruments. Through data analysis, findings of the study have shed light on the fact that capacity building constitutes an indispensable mechanism for local government to achieve its developmental mandate, despite challenges associated with the process of capacity building. The study found that the Kicukiro district has made a significant progress towards the implementation of capacity-building interventions despite the fact that these interventions are still fragmented, uncoordinated and still supply-driven. Furthermore, the study revealed that the capacities already built generated considerable impetus that contributed to socio-economic development within the Kicukiro district. The study also found that despite the progress made in both capacity building and development in Kicukiro district, there are capacity gaps and factors which are undermining further progress in this regard. Therefore, the study recommends, among others, an effective co-ordination of capacity-building interventions in order to avoid duplication and fragmentation of capacity-building efforts.
- Full Text:
- Date Issued: 2015
- Authors: Rutebuka, Balinda
- Date: 2015
- Subjects: Sustainable development -- Rwanda , Economic development -- Rwanda , Local government -- Rwanda
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: vital:8490 , http://hdl.handle.net/10948/d1020906
- Description: This study investigated the capacity building for developmental local government with reference to the Kicukiro District of Rwanda. Its aim was to examine the contribution of capacity-building interventions towards a developmental local government and at the same time investigate factors that hinder their implementation in Kicukiro district. The study provides an extensive historical background on local government in Rwa-nda with a particular focus on its policy, legal and institutional framework, whereby the developmental local government environment has been analysed. Furthermore, this study explores the theoretical framework of capacity building in general, and in this regard particular emphasis was given to capacity building in relation to developmental local government. The study also argued that without appropriate capacity it would be difficult for the district to fulfil its developmental mandate. This research followed both qualitative and quantitative approaches of study. It involved a survey in which a structured questionnaire and in-depth interviews were used as data gathering instruments. Through data analysis, findings of the study have shed light on the fact that capacity building constitutes an indispensable mechanism for local government to achieve its developmental mandate, despite challenges associated with the process of capacity building. The study found that the Kicukiro district has made a significant progress towards the implementation of capacity-building interventions despite the fact that these interventions are still fragmented, uncoordinated and still supply-driven. Furthermore, the study revealed that the capacities already built generated considerable impetus that contributed to socio-economic development within the Kicukiro district. The study also found that despite the progress made in both capacity building and development in Kicukiro district, there are capacity gaps and factors which are undermining further progress in this regard. Therefore, the study recommends, among others, an effective co-ordination of capacity-building interventions in order to avoid duplication and fragmentation of capacity-building efforts.
- Full Text:
- Date Issued: 2015
Capacity building for local economic development: an evaluation of training initiatives in the Cape Winelands district
- Authors: Kamara, Richard Douglas
- Date: 2015
- Subjects: Economic development projects -- South Africa , Community development -- South Africa , Employees -- Training of
- Language: English
- Type: Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10948/7969 , vital:24331
- Description: The paradigmatic shifts in development approaches epitomised by contemporary discourses about development confer a prominent role on localised and territorial development. A growing scepticism on the efficacy of traditional development approaches provides the impetus for a strong understanding of the need to reconceptualise development theory and practices and to manipulate policies so as to remedy the imbalances of antecedent development approaches. This dissertation investigates the extent to which the existing training interventions can build skill capacity for Local Economic Development (LED) projects in the Cape Winelands municipalities. This will improve our understanding about how, and under what conditions, capacity building for LED can contribute to more inclusive economic and social change. In discussing the theoretical perspective of the study, the relationship between development, LED and capacity building is conceptualised through the lens of contemporary development theory of human development and capability approach. This will improve our understanding on how the capability approach aspires to re-orient approaches to socio-economic development and public policy, away from welfare, which is based on income and expenditure to well-being. The dissertation applies these ideas triangulating diverse research methods and data sources. It combines a literature review and documentary analysis, observation, surveys conducted with municipal authorities in Cape Winelands District Municipality. In addition, semi-structured interviews were held with LED Portfolio Councillors in the municipality as well as with key Officers from Local Government Sector for Education and Training Authority, Department of Economic Development and Tourism in Western Cape, South African Local Government Association and Department of Co-operative Governance and Traditional Affairs. The methodological findings reveals the following pitfalls: Flaws in LED enabling policy framework for capacitating municipal staff; poor policy implementations; training fund difficult to access; lack of competent staff in local municipalities to effectively and efficiently implement LED policies and strategies; and lack of supportive environment in workplace to enhance transfer of trained skill to the job. Various recommendations resulting from the outcomes of the empirical study, namely the responses made by the respondents during the empirical survey, are proposed in the final chapter. It is shown that this research has, as a result, contributed to the body of knowledge of development theory and practices by improving our understanding of how, and under which conditions, capacity building training can support processes of social change in localised and territorial development.
- Full Text:
- Date Issued: 2015
- Authors: Kamara, Richard Douglas
- Date: 2015
- Subjects: Economic development projects -- South Africa , Community development -- South Africa , Employees -- Training of
- Language: English
- Type: Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10948/7969 , vital:24331
- Description: The paradigmatic shifts in development approaches epitomised by contemporary discourses about development confer a prominent role on localised and territorial development. A growing scepticism on the efficacy of traditional development approaches provides the impetus for a strong understanding of the need to reconceptualise development theory and practices and to manipulate policies so as to remedy the imbalances of antecedent development approaches. This dissertation investigates the extent to which the existing training interventions can build skill capacity for Local Economic Development (LED) projects in the Cape Winelands municipalities. This will improve our understanding about how, and under what conditions, capacity building for LED can contribute to more inclusive economic and social change. In discussing the theoretical perspective of the study, the relationship between development, LED and capacity building is conceptualised through the lens of contemporary development theory of human development and capability approach. This will improve our understanding on how the capability approach aspires to re-orient approaches to socio-economic development and public policy, away from welfare, which is based on income and expenditure to well-being. The dissertation applies these ideas triangulating diverse research methods and data sources. It combines a literature review and documentary analysis, observation, surveys conducted with municipal authorities in Cape Winelands District Municipality. In addition, semi-structured interviews were held with LED Portfolio Councillors in the municipality as well as with key Officers from Local Government Sector for Education and Training Authority, Department of Economic Development and Tourism in Western Cape, South African Local Government Association and Department of Co-operative Governance and Traditional Affairs. The methodological findings reveals the following pitfalls: Flaws in LED enabling policy framework for capacitating municipal staff; poor policy implementations; training fund difficult to access; lack of competent staff in local municipalities to effectively and efficiently implement LED policies and strategies; and lack of supportive environment in workplace to enhance transfer of trained skill to the job. Various recommendations resulting from the outcomes of the empirical study, namely the responses made by the respondents during the empirical survey, are proposed in the final chapter. It is shown that this research has, as a result, contributed to the body of knowledge of development theory and practices by improving our understanding of how, and under which conditions, capacity building training can support processes of social change in localised and territorial development.
- Full Text:
- Date Issued: 2015
Cape mountain zebra (Equus zebra zebra) habitat use and diet in the Bontebok National Park, South Africa
- Authors: Strauss, Taniia
- Date: 2015
- Subjects: Habitat conservation -- South Africa -- Western Cape Zebras -- Habitat -- Conservation -- South Africa -- Western Cape
- Language: English
- Type: Thesis , Masters , MTech
- Identifier: http://hdl.handle.net/10948/11374 , vital:26917
- Description: Cape mountain zebra habitat utilization and diet in the dystrophic fynbos habitat of the Bontebok National Park was found to be highly selective. Mountain zebra concentrated in specific sectors and habitat types in the park on a seasonal basis, preferring recently burnt habitat with a veld age younger than one year in all seasons, except during the warm, dry summer. Proteoid Fynbos with a veld age between one and five years was preferred in the cool winter, while Drainage Lines and the fringes of Inland Pans were preferred during summer. Asteraceous Fynbos was avoided, irrespective of veld age, as well as all other habitats with a veld age greater than five years. Within habitat types with a veld age greater than one year, specific sites were selected and avoided on a seasonal basis, which were found to differ in terms of habitat suitability, based on the availability of dietary plant species. The annual diet consisted of 72.6% grass, 11.8% restio, 5.9% sedge, 8.8% geophyte and less than 1% forb and shrub species. Three grass species formed the bulk of the annual diet, Themeda triandra, Cymbopogon marginatus and Eragrostis curvula, for which leaf use was greater than stem use. Themeda trianda was preferred throughout the year, but contributed to the diet in greatest proportion in the warm, dry summer, when it was available at greatest leaf height and diameter. Cymbopogon marginatus was preferred during the cool winter, when diet composition and greenness was also greater than in other seasons. Preference of Cymbopogon marginatus decreased as leaf height and diameter increased. Eragrostis curvula was preferred in the warm autumn, when it composed the largest proportion of the diet, and selection of this species at feeding sites was based on both greenness and volume. In summer mountain zebra also preferred grass stems and inflorescences of Aristida diffusa, Stipagrostis zeyheri and Briza maxima. During the cool spring the diet included stems and inflorescences of sedges and restios, primarily Ischyrolepis capensis, and in autumn, dry bulbs of the geophyte Moraea collina were utilized. Habitat utilization, as well as grass height and greenness surveys in the Recently Burnt Area, and the nutritional status of mountain zebra was found to be in line with the Summer Nutritional Stress Hypothesis. The hypothesis proposes that the harsh climatic conditions of the area during summer are linked to the low availability of C4 grass, on which grazers would depend in summer. This is supported by the avoidance of the Recently Burnt Area in summer, and the preference of species like Themeda triandra during summer despite low greenness levels. Faecal nitrogen and phosphorus for mountain zebra in Bontebok National Park and De Hoop Nature Reserve were at minimum levels during the warm seasons. Faecal nitrogen was below the threshold for dietary deficiency in all seasons except spring, and faecal phosphorus was above the threshold for deficiency during two seasons only. The findings of this study are in line with other recent work on mountain zebra in the Baviaanskloof suggesting that, due to a high required rate of forage intake, mountain zebra are limited by both poor resource quantity and quality in dystrophic fynbos ecosystems.
- Full Text:
- Date Issued: 2015
- Authors: Strauss, Taniia
- Date: 2015
- Subjects: Habitat conservation -- South Africa -- Western Cape Zebras -- Habitat -- Conservation -- South Africa -- Western Cape
- Language: English
- Type: Thesis , Masters , MTech
- Identifier: http://hdl.handle.net/10948/11374 , vital:26917
- Description: Cape mountain zebra habitat utilization and diet in the dystrophic fynbos habitat of the Bontebok National Park was found to be highly selective. Mountain zebra concentrated in specific sectors and habitat types in the park on a seasonal basis, preferring recently burnt habitat with a veld age younger than one year in all seasons, except during the warm, dry summer. Proteoid Fynbos with a veld age between one and five years was preferred in the cool winter, while Drainage Lines and the fringes of Inland Pans were preferred during summer. Asteraceous Fynbos was avoided, irrespective of veld age, as well as all other habitats with a veld age greater than five years. Within habitat types with a veld age greater than one year, specific sites were selected and avoided on a seasonal basis, which were found to differ in terms of habitat suitability, based on the availability of dietary plant species. The annual diet consisted of 72.6% grass, 11.8% restio, 5.9% sedge, 8.8% geophyte and less than 1% forb and shrub species. Three grass species formed the bulk of the annual diet, Themeda triandra, Cymbopogon marginatus and Eragrostis curvula, for which leaf use was greater than stem use. Themeda trianda was preferred throughout the year, but contributed to the diet in greatest proportion in the warm, dry summer, when it was available at greatest leaf height and diameter. Cymbopogon marginatus was preferred during the cool winter, when diet composition and greenness was also greater than in other seasons. Preference of Cymbopogon marginatus decreased as leaf height and diameter increased. Eragrostis curvula was preferred in the warm autumn, when it composed the largest proportion of the diet, and selection of this species at feeding sites was based on both greenness and volume. In summer mountain zebra also preferred grass stems and inflorescences of Aristida diffusa, Stipagrostis zeyheri and Briza maxima. During the cool spring the diet included stems and inflorescences of sedges and restios, primarily Ischyrolepis capensis, and in autumn, dry bulbs of the geophyte Moraea collina were utilized. Habitat utilization, as well as grass height and greenness surveys in the Recently Burnt Area, and the nutritional status of mountain zebra was found to be in line with the Summer Nutritional Stress Hypothesis. The hypothesis proposes that the harsh climatic conditions of the area during summer are linked to the low availability of C4 grass, on which grazers would depend in summer. This is supported by the avoidance of the Recently Burnt Area in summer, and the preference of species like Themeda triandra during summer despite low greenness levels. Faecal nitrogen and phosphorus for mountain zebra in Bontebok National Park and De Hoop Nature Reserve were at minimum levels during the warm seasons. Faecal nitrogen was below the threshold for dietary deficiency in all seasons except spring, and faecal phosphorus was above the threshold for deficiency during two seasons only. The findings of this study are in line with other recent work on mountain zebra in the Baviaanskloof suggesting that, due to a high required rate of forage intake, mountain zebra are limited by both poor resource quantity and quality in dystrophic fynbos ecosystems.
- Full Text:
- Date Issued: 2015
Carbonate petrography and geochemistry of BIF of the Transvaal supergroup : evaluating the potential of iron carbonates as proxies for palaeoproterozoic ocean chemistry
- Authors: Rafuza, Sipesihle
- Date: 2015
- Subjects: Carbonate rocks -- South Africa -- Transvaal Supergroup , Petrology -- South Africa -- Transvaal Supergroup , Geochemistry -- South Africa -- Transvaal Supergroup , Petrology -- South Africa -- Kuruman , Petrology -- South Africa -- Griekwastad , Geology, Stratigraphic -- Proterozoic , Chemical oceanography , Iron
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5089 , http://hdl.handle.net/10962/d1018611
- Description: The subject of BIF genesis, particularly their environmental conditions and ocean chemistry at the time of deposition and their evolution through time, has been a subject of much contentiousness, generating a wealth of proposed genetic models and constant refinements thereof over the years. The prevailing paradigm within the various schools of thought, is the widespread and generally agreed upon depositional and diagenetic model(s) which advocate for BIF deposition under anoxic marine conditions. According to the prevailing models, the primary depositional environment would have involved a seawater column whereby soluble Fe²⁺ expelled by hydrothermal activity mixed with free O₂ from the shallow photic zone produced by eukaryotes, forming a high valence iron oxy-hydroxide precursor such as FeOOH or Fe(OH)₃. An alternative biological mechanism producing similar ferric precursors would have been in the form of photo-ferrotrophy, whereby oxidation of ferrous iron to the ferric form took place in the absence of biological O₂ production. Irrespective of the exact mode of primary iron precipitation (which remains contentious to date), the precipitated ferric oxy-hydroxide precursor would have reacted with co-precipitated organic matter, thus acting as a suitable electron acceptor for organic carbon remineralisation through Dissimilatory Iron Reduction (DIR), as also observed in many modern anoxic diagenetic environments. DIR-dominated diagenetic models imply a predominantly diagenetic influence in BIF mineralogy and genesis, and use as key evidence the low δ¹³C values relative to the seawater bicarbonate value of ~0 ‰, which is also thought to have been the dissolved bicarbonate isotope composition in the early Precambrian oceans. The carbon for diagenetic carbonate formation would thus have been sourced through a combination of two end-member sources: pore-fluid bicarbonate at ~0 ‰ and particulate organic carbon at circa -28 ‰, resulting in the intermediate δ¹³C values observed in BIFs today. This study targets 65 drillcore samples of the upper Kuruman and Griquatown BIF from the lower Transvaal Supergroup in the Hotazel area, Northern Cape, South Africa, and sets out to explore key aspects in BIF carbonate petrography and geochemistry that are pertinent to current debates surrounding their interpretation with regard to primary versus diagenetic processes. The focus here rests on applications of carbonate (mainly siderite and ankerite) petrography, mineral chemistry, bulk and mineral-specific carbon isotopes and speciation analyses, with a view to obtaining valuable new insights into BIF carbonates as potential records of ocean chemistry for their bulk carbonate-carbon isotope signature. Evaluation of the present results is done in light of pre-existing, widely accepted diagenetic models against a proposed water-column model for the origin of the carbonate species in BIF. The latter utilises a combination of geochemical attributes of the studied carbonates, including the conspicuous Mn enrichment and stratigraphic variability in Mn/Fe ratio of the Griquatown BIF recorded solely in the carbonate fraction of the rocks. Additionally, the carbon isotope signatures of the Griquatown BIF samples are brought into the discussion and provide insights into the potential causes and mechanisms that may have controlled these signatures in a diagenetic versus primary sedimentary environment. Ultimately, implications of the combined observations, findings and arguments presented in this thesis are presented and discussed with particular respect to the redox evolution and carbon cycle of the ocean system prior to the Great Oxidation Event (GOE). A crucial conclusion reached is that, by contrast to previously-proposed models, diagenesis cannot singularly be the major contributing factor in BIF genesis at least with respect to the carbonate fraction in BIF, as it does not readily explain the carbon isotope and mineral-chemical signatures of carbonates in the Griquatown and uppermost Kuruman BIFs. It is proposed instead that these signatures may well record water-column processes of carbon, manganese and iron cycling, and that carbonate formation in the water column and its subsequent transfer to the precursor BIF sediment constitutes a faithful record of such processes. Corollary to that interpretation is the suggestion that the evidently increasing Mn abundance in the carbonate fraction of the Griquatown BIF up-section would point to a chemically evolving depositional basin with time, from being mainly ferruginous as expressed by Mn-poor BIFs in the lower stratigraphic sections (i.e. Kuruman BF) to more manganiferous as recorded in the upper Griquatown BIF, culminating in the deposition of the abnormally enriched in Mn Hotazel BIF at the stratigraphic top of the Transvaal Supergroup. The Paleoproterozoic ocean must therefore have been characterised by long-term active cycling of organic carbon in the water column in the form of an ancient biological pump, albeit with Fe(III) and subsequently Mn(III,IV) oxy-hydroxides being the key electron acceptors within the water column. The highly reproducible stratigraphic isotope profiles for bulk δ¹³C from similar sections further afield over distances up to 20 km, further corroborate unabatedly that bulk carbonate carbon isotope signatures record water column carbon cycling processes rather than widely-proposed anaerobic diagenetic processes.
- Full Text:
- Date Issued: 2015
- Authors: Rafuza, Sipesihle
- Date: 2015
- Subjects: Carbonate rocks -- South Africa -- Transvaal Supergroup , Petrology -- South Africa -- Transvaal Supergroup , Geochemistry -- South Africa -- Transvaal Supergroup , Petrology -- South Africa -- Kuruman , Petrology -- South Africa -- Griekwastad , Geology, Stratigraphic -- Proterozoic , Chemical oceanography , Iron
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5089 , http://hdl.handle.net/10962/d1018611
- Description: The subject of BIF genesis, particularly their environmental conditions and ocean chemistry at the time of deposition and their evolution through time, has been a subject of much contentiousness, generating a wealth of proposed genetic models and constant refinements thereof over the years. The prevailing paradigm within the various schools of thought, is the widespread and generally agreed upon depositional and diagenetic model(s) which advocate for BIF deposition under anoxic marine conditions. According to the prevailing models, the primary depositional environment would have involved a seawater column whereby soluble Fe²⁺ expelled by hydrothermal activity mixed with free O₂ from the shallow photic zone produced by eukaryotes, forming a high valence iron oxy-hydroxide precursor such as FeOOH or Fe(OH)₃. An alternative biological mechanism producing similar ferric precursors would have been in the form of photo-ferrotrophy, whereby oxidation of ferrous iron to the ferric form took place in the absence of biological O₂ production. Irrespective of the exact mode of primary iron precipitation (which remains contentious to date), the precipitated ferric oxy-hydroxide precursor would have reacted with co-precipitated organic matter, thus acting as a suitable electron acceptor for organic carbon remineralisation through Dissimilatory Iron Reduction (DIR), as also observed in many modern anoxic diagenetic environments. DIR-dominated diagenetic models imply a predominantly diagenetic influence in BIF mineralogy and genesis, and use as key evidence the low δ¹³C values relative to the seawater bicarbonate value of ~0 ‰, which is also thought to have been the dissolved bicarbonate isotope composition in the early Precambrian oceans. The carbon for diagenetic carbonate formation would thus have been sourced through a combination of two end-member sources: pore-fluid bicarbonate at ~0 ‰ and particulate organic carbon at circa -28 ‰, resulting in the intermediate δ¹³C values observed in BIFs today. This study targets 65 drillcore samples of the upper Kuruman and Griquatown BIF from the lower Transvaal Supergroup in the Hotazel area, Northern Cape, South Africa, and sets out to explore key aspects in BIF carbonate petrography and geochemistry that are pertinent to current debates surrounding their interpretation with regard to primary versus diagenetic processes. The focus here rests on applications of carbonate (mainly siderite and ankerite) petrography, mineral chemistry, bulk and mineral-specific carbon isotopes and speciation analyses, with a view to obtaining valuable new insights into BIF carbonates as potential records of ocean chemistry for their bulk carbonate-carbon isotope signature. Evaluation of the present results is done in light of pre-existing, widely accepted diagenetic models against a proposed water-column model for the origin of the carbonate species in BIF. The latter utilises a combination of geochemical attributes of the studied carbonates, including the conspicuous Mn enrichment and stratigraphic variability in Mn/Fe ratio of the Griquatown BIF recorded solely in the carbonate fraction of the rocks. Additionally, the carbon isotope signatures of the Griquatown BIF samples are brought into the discussion and provide insights into the potential causes and mechanisms that may have controlled these signatures in a diagenetic versus primary sedimentary environment. Ultimately, implications of the combined observations, findings and arguments presented in this thesis are presented and discussed with particular respect to the redox evolution and carbon cycle of the ocean system prior to the Great Oxidation Event (GOE). A crucial conclusion reached is that, by contrast to previously-proposed models, diagenesis cannot singularly be the major contributing factor in BIF genesis at least with respect to the carbonate fraction in BIF, as it does not readily explain the carbon isotope and mineral-chemical signatures of carbonates in the Griquatown and uppermost Kuruman BIFs. It is proposed instead that these signatures may well record water-column processes of carbon, manganese and iron cycling, and that carbonate formation in the water column and its subsequent transfer to the precursor BIF sediment constitutes a faithful record of such processes. Corollary to that interpretation is the suggestion that the evidently increasing Mn abundance in the carbonate fraction of the Griquatown BIF up-section would point to a chemically evolving depositional basin with time, from being mainly ferruginous as expressed by Mn-poor BIFs in the lower stratigraphic sections (i.e. Kuruman BF) to more manganiferous as recorded in the upper Griquatown BIF, culminating in the deposition of the abnormally enriched in Mn Hotazel BIF at the stratigraphic top of the Transvaal Supergroup. The Paleoproterozoic ocean must therefore have been characterised by long-term active cycling of organic carbon in the water column in the form of an ancient biological pump, albeit with Fe(III) and subsequently Mn(III,IV) oxy-hydroxides being the key electron acceptors within the water column. The highly reproducible stratigraphic isotope profiles for bulk δ¹³C from similar sections further afield over distances up to 20 km, further corroborate unabatedly that bulk carbonate carbon isotope signatures record water column carbon cycling processes rather than widely-proposed anaerobic diagenetic processes.
- Full Text:
- Date Issued: 2015
Case study : profitability drivers in the South African airline industry : a comparative analysis of SAA and Comair
- Authors: Batidzirai, Davison Herbert
- Date: 2015
- Subjects: South African Airways , Comair Limited , Airlines -- South Africa , Corporate profits -- South Africa , Organizational effectiveness -- South Africa
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: vital:846 , http://hdl.handle.net/10962/d1017191
- Full Text:
- Date Issued: 2015
- Authors: Batidzirai, Davison Herbert
- Date: 2015
- Subjects: South African Airways , Comair Limited , Airlines -- South Africa , Corporate profits -- South Africa , Organizational effectiveness -- South Africa
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: vital:846 , http://hdl.handle.net/10962/d1017191
- Full Text:
- Date Issued: 2015
Challenges faced by Teachers in applying reading strategies when teaching English reading in the foundation phase in the Mthatha District
- Authors: Cweba, Vuyokazi
- Date: 2015
- Subjects: Reading(Foundation phase) English language -- Study and teaching -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Doctoral , D Ed
- Identifier: http://hdl.handle.net/11260/856 , vital:29938
- Description: This study sought to investigate challenges faced by teachers in applying reading strategies when teaching reading in the Foundation Phase in the Mthatha District in South Africa. The study was conducted at Four Junior Secondary Schools. It was prompted by a deep concern about challenges faced by teachers in applying reading strategies required in the Foundation Phase. While studies have been carried out on applying reading strategies in the Foundation Phase, few studies have looked at the factors impacting on reading in South African schools, especially in the Mthatha District where isiXhosa is the mother tongue. The method used in this study involved a qualitative approach. The study addressed the following research questions: What strategies are used in the Foundation Phase? What type of challenges are faced by teachers? To what extent are educators trained to teach reading in the Foundation Phase? Does the lack of resources affect the performance of learners? To what extent does the use of a second language affect the performance of Foundation Phase learners? Do teachers receive enough support from the Department of Education to teach reading? The study is a multiple-case study and data were collected through individual interviews and focus group interviews (sixteen teachers were interviewed). Content analysis guided the data analysis through themes derived from the subsidiary questions. Data reporting took the form of thick description and verbatim quotations in line with the qualitative approach of the study. A pilot study was conducted in two Junior Secondary Schools for the purpose of validity and reliability. ii The study`s findings revealed that: Different reading strategies were used by teachers in the Foundation Phase, teachers faced many challenges in applying reading strategies such as teacher training in the teaching of reading. This suggests that intervention is needed by the Department of Education such as providing relevant resources which are essential in order to address the problem of reading. Improvisation by teachers to improve reading material, insufficient allocation of time, multilingualism, teacher competence, lack of motivation and parental involvement in relation to learner performance in reading all need attention in order to solve problems. Furthermore, lack of resources hinders the reading ability of learners. The use of home language as a medium of instruction in teaching reading is highlighted. There is insufficient support by the Department of Education for Foundation Phase teachers to teach reading. Based on the study findings, the researcher has recommended that teachers should be urged to use reading strategies in a way that benefits learners. The use of reading strategies, therefore, needs more attention. Libraries should be built in these schools to enable learners to develop a culture of reading. Education development officers, subject advisors, lead teachers and parents should work together to improve the reading ability of Foundation Phase learners.
- Full Text:
- Date Issued: 2015
- Authors: Cweba, Vuyokazi
- Date: 2015
- Subjects: Reading(Foundation phase) English language -- Study and teaching -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Doctoral , D Ed
- Identifier: http://hdl.handle.net/11260/856 , vital:29938
- Description: This study sought to investigate challenges faced by teachers in applying reading strategies when teaching reading in the Foundation Phase in the Mthatha District in South Africa. The study was conducted at Four Junior Secondary Schools. It was prompted by a deep concern about challenges faced by teachers in applying reading strategies required in the Foundation Phase. While studies have been carried out on applying reading strategies in the Foundation Phase, few studies have looked at the factors impacting on reading in South African schools, especially in the Mthatha District where isiXhosa is the mother tongue. The method used in this study involved a qualitative approach. The study addressed the following research questions: What strategies are used in the Foundation Phase? What type of challenges are faced by teachers? To what extent are educators trained to teach reading in the Foundation Phase? Does the lack of resources affect the performance of learners? To what extent does the use of a second language affect the performance of Foundation Phase learners? Do teachers receive enough support from the Department of Education to teach reading? The study is a multiple-case study and data were collected through individual interviews and focus group interviews (sixteen teachers were interviewed). Content analysis guided the data analysis through themes derived from the subsidiary questions. Data reporting took the form of thick description and verbatim quotations in line with the qualitative approach of the study. A pilot study was conducted in two Junior Secondary Schools for the purpose of validity and reliability. ii The study`s findings revealed that: Different reading strategies were used by teachers in the Foundation Phase, teachers faced many challenges in applying reading strategies such as teacher training in the teaching of reading. This suggests that intervention is needed by the Department of Education such as providing relevant resources which are essential in order to address the problem of reading. Improvisation by teachers to improve reading material, insufficient allocation of time, multilingualism, teacher competence, lack of motivation and parental involvement in relation to learner performance in reading all need attention in order to solve problems. Furthermore, lack of resources hinders the reading ability of learners. The use of home language as a medium of instruction in teaching reading is highlighted. There is insufficient support by the Department of Education for Foundation Phase teachers to teach reading. Based on the study findings, the researcher has recommended that teachers should be urged to use reading strategies in a way that benefits learners. The use of reading strategies, therefore, needs more attention. Libraries should be built in these schools to enable learners to develop a culture of reading. Education development officers, subject advisors, lead teachers and parents should work together to improve the reading ability of Foundation Phase learners.
- Full Text:
- Date Issued: 2015
Challenges of post-apartheid state-owned company pension fund reform: a case study of the controversy around the Transnet-Transport Pension Fund
- Authors: Goqoza, Noluyolo Juliet
- Date: 2015
- Language: English
- Type: text , Thesis , Masters , MSocSc
- Identifier: http://hdl.handle.net/10962/54766 , vital:26610
- Description: This thesis examines the restructuring of the pension funds of Transnet, a South African state-owned company involved in transportation, from the 1990s. Two of its main pension funds, the Transport-Transnet Pension sub-Fund (TTPF) and the Transnet Second Defined Benefit Fund (TSDBF), have been surrounded by controversy, with major court actions brought by aggrieved pensioners in 2006-2012 and again from 2013, and smaller cases in 1997-1999 and 2004. (There were also a number of smaller cases, mostly unsuccessful, but the thesis will not examine them). The case that started in 2013 is the biggest class action in the country‟s history, and makes claims of serious mismanagement and bad faith against the Transnet management. But the fundamental grievance is that (according to the 2013 legal case) “more than 80% of pensioners earn less than R4 000.00 a month… 62 % earn less than R2 500.00… 45% of the pensioners earn less than the state‟s ordinary old-age pension” grant for the poor. Although that case is ongoing, this thesis examines the background and controversies that frame the case. It provides an overview of the history and development of the South African pensions system and South African state-owned companies; it examines how these have been shaped by the apartheid and post-apartheid periods, and by the rise of neo-liberalism; it examines the evolution of Transnet and its pensions systems, from the early days of the South African Railways and Harbours Administration (SAR&H, formed 1910), to its restructuring into the South African Transport Services (SATS) in 1982, and then into Transnet in 1990. The thesis shows that the operations of the TTPF and TSDBF, which are closed to new members, have had serious effects on pensioners that rely upon them. Pensions are very low (the main reason for the various court cases), and this is for a range of reasons. Annual increases in pensions are formally set at below-inflation levels, leading to falling real incomes. More pressure on pensioners‟ livelihoods has arisen from Transnet‟s cuts to other benefits, like the medical aid Transmed, provided to pensioners. While the schemes are solvent, the pensions generally started at a low base, partly because most pensioners were relatively poorly paid workers before retirement (and the pensions were linked to former salaries). There is also a racial dimension: while most white workers at SAR&H/ SATS and Transnet were poorly paid, black, Coloured and Indian workers were paid even worse, and, further, were only brought into the pension schemes late. Both TTPF and TSDBF are defined benefit funds, which means members are guaranteed specific benefits at retirement, with the employer obligated to inject funds to meet shortfalls where needed. Yet neither the state nor Transnet has been willing to take actions to lift the basic pensions, such as investments into the funds, or to make systematic ex gratia payments to bring the pensions to a reasonable level, to remove historic racial inequalities between pensioners, to increase medical aid co-payments or coverage or to otherwise address the pensioners‟ situation. It does not seem that the reason for the problems is that the two funds have been severely mismanaged or asset-stripped, as alleged in the 2013 class action: it must be noted that both funds report surpluses. But the surpluses are possible because the pensions are low and falling in real terms, and the numbers of pensioners declining due to deaths. It seems clear that Transnet is unable or unwilling to act to decisively improve the situation of the pensioners: ensuring a surplus on existing pension funds is a major goal. This is partly because Transnet itself has ongoing financial problems, and partly because it operates in the context of neo-liberal restructuring, like corporatisation, commercialisation and privatisation, which places limits on the additional funding of the funds. At the same time, the pensioners have very little real, as opposed to a nominal, say in the administration of the pension schemes, limiting their ability to affect the rules and administration or raise issues. The thesis seeks to use historical institutionalism, which sees policies and major institutions, including state-owned companies, as shaped by power and conflict, especially between classes. This is used to try and explain changing state policies and the changing role and actions of state-owned companies, as a way of understanding Transnet‟s actions, as well as its treatment of its pensioners.
- Full Text:
- Date Issued: 2015
- Authors: Goqoza, Noluyolo Juliet
- Date: 2015
- Language: English
- Type: text , Thesis , Masters , MSocSc
- Identifier: http://hdl.handle.net/10962/54766 , vital:26610
- Description: This thesis examines the restructuring of the pension funds of Transnet, a South African state-owned company involved in transportation, from the 1990s. Two of its main pension funds, the Transport-Transnet Pension sub-Fund (TTPF) and the Transnet Second Defined Benefit Fund (TSDBF), have been surrounded by controversy, with major court actions brought by aggrieved pensioners in 2006-2012 and again from 2013, and smaller cases in 1997-1999 and 2004. (There were also a number of smaller cases, mostly unsuccessful, but the thesis will not examine them). The case that started in 2013 is the biggest class action in the country‟s history, and makes claims of serious mismanagement and bad faith against the Transnet management. But the fundamental grievance is that (according to the 2013 legal case) “more than 80% of pensioners earn less than R4 000.00 a month… 62 % earn less than R2 500.00… 45% of the pensioners earn less than the state‟s ordinary old-age pension” grant for the poor. Although that case is ongoing, this thesis examines the background and controversies that frame the case. It provides an overview of the history and development of the South African pensions system and South African state-owned companies; it examines how these have been shaped by the apartheid and post-apartheid periods, and by the rise of neo-liberalism; it examines the evolution of Transnet and its pensions systems, from the early days of the South African Railways and Harbours Administration (SAR&H, formed 1910), to its restructuring into the South African Transport Services (SATS) in 1982, and then into Transnet in 1990. The thesis shows that the operations of the TTPF and TSDBF, which are closed to new members, have had serious effects on pensioners that rely upon them. Pensions are very low (the main reason for the various court cases), and this is for a range of reasons. Annual increases in pensions are formally set at below-inflation levels, leading to falling real incomes. More pressure on pensioners‟ livelihoods has arisen from Transnet‟s cuts to other benefits, like the medical aid Transmed, provided to pensioners. While the schemes are solvent, the pensions generally started at a low base, partly because most pensioners were relatively poorly paid workers before retirement (and the pensions were linked to former salaries). There is also a racial dimension: while most white workers at SAR&H/ SATS and Transnet were poorly paid, black, Coloured and Indian workers were paid even worse, and, further, were only brought into the pension schemes late. Both TTPF and TSDBF are defined benefit funds, which means members are guaranteed specific benefits at retirement, with the employer obligated to inject funds to meet shortfalls where needed. Yet neither the state nor Transnet has been willing to take actions to lift the basic pensions, such as investments into the funds, or to make systematic ex gratia payments to bring the pensions to a reasonable level, to remove historic racial inequalities between pensioners, to increase medical aid co-payments or coverage or to otherwise address the pensioners‟ situation. It does not seem that the reason for the problems is that the two funds have been severely mismanaged or asset-stripped, as alleged in the 2013 class action: it must be noted that both funds report surpluses. But the surpluses are possible because the pensions are low and falling in real terms, and the numbers of pensioners declining due to deaths. It seems clear that Transnet is unable or unwilling to act to decisively improve the situation of the pensioners: ensuring a surplus on existing pension funds is a major goal. This is partly because Transnet itself has ongoing financial problems, and partly because it operates in the context of neo-liberal restructuring, like corporatisation, commercialisation and privatisation, which places limits on the additional funding of the funds. At the same time, the pensioners have very little real, as opposed to a nominal, say in the administration of the pension schemes, limiting their ability to affect the rules and administration or raise issues. The thesis seeks to use historical institutionalism, which sees policies and major institutions, including state-owned companies, as shaped by power and conflict, especially between classes. This is used to try and explain changing state policies and the changing role and actions of state-owned companies, as a way of understanding Transnet‟s actions, as well as its treatment of its pensioners.
- Full Text:
- Date Issued: 2015
Challenges to service delivery in the Department of Home Affairs
- Authors: Mahlungulu, Zimkitha
- Date: 2015
- Subjects: Public administration -- South Africa -- Eastern Cape , Customer services -- South Africa -- Eastern Cape , South Africa. Department of Home Affairs
- Language: English
- Type: Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10948/6573 , vital:21114
- Description: The South African public sector is important for the sustainable growth and development of the country. One of its major responsibilities is to ensure that all citizens have access to and receive services. Achieving a high degree of productivity is an important objective of public service organisations across the world, given the pressure to deliver quality public goods and services within the limits of ever-increasing resource constraints. The South African public service is no exception to this global phenomenon. The challenge of the public service is therefore to continuously improve performance in order to meet citizens’ needs. The focus of this study is the challenges facing service delivery in the Department of Home Affairs. The aim of this study is to explore and describe the challenges that hinder full transformation and maximum customer/client satisfaction in regards to service delivery offered by the Department of Home Affairs. The objectives are: to identify the challenges experienced by staff offering services to clients at the Department of Home Affairs and to identify the problems experienced by clients who receive services at Home Affairs. The study employs a qualitative research methodology and uses observation and individual interviews as data collections tools. The findings from both sources indicate that the clients were not happy with the quality of service they receive at the ID section. Amongst other things, they complained about lack of information, lack of guidance, unprofessional staff, and technical problems that they had experienced. However, the staff also encountered a number of challenges, including being short staffed, lack of resources, and system problems as the system is new to them and they have not received enough training before it was implemented.
- Full Text:
- Date Issued: 2015
- Authors: Mahlungulu, Zimkitha
- Date: 2015
- Subjects: Public administration -- South Africa -- Eastern Cape , Customer services -- South Africa -- Eastern Cape , South Africa. Department of Home Affairs
- Language: English
- Type: Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10948/6573 , vital:21114
- Description: The South African public sector is important for the sustainable growth and development of the country. One of its major responsibilities is to ensure that all citizens have access to and receive services. Achieving a high degree of productivity is an important objective of public service organisations across the world, given the pressure to deliver quality public goods and services within the limits of ever-increasing resource constraints. The South African public service is no exception to this global phenomenon. The challenge of the public service is therefore to continuously improve performance in order to meet citizens’ needs. The focus of this study is the challenges facing service delivery in the Department of Home Affairs. The aim of this study is to explore and describe the challenges that hinder full transformation and maximum customer/client satisfaction in regards to service delivery offered by the Department of Home Affairs. The objectives are: to identify the challenges experienced by staff offering services to clients at the Department of Home Affairs and to identify the problems experienced by clients who receive services at Home Affairs. The study employs a qualitative research methodology and uses observation and individual interviews as data collections tools. The findings from both sources indicate that the clients were not happy with the quality of service they receive at the ID section. Amongst other things, they complained about lack of information, lack of guidance, unprofessional staff, and technical problems that they had experienced. However, the staff also encountered a number of challenges, including being short staffed, lack of resources, and system problems as the system is new to them and they have not received enough training before it was implemented.
- Full Text:
- Date Issued: 2015
Change management: transformational issues in South-African rugby
- Authors: Erasmus, Innis
- Date: 2015
- Subjects: Organizational change -- Management , Rugby football -- South Africa , Sports administration
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: http://hdl.handle.net/10948/48819 , vital:41143
- Description: The history of the sport rugby evolved from a football game more than a century ago, in England, in a town called Rugby when a young boy by the name of William Webb Ellis picked up the ball and ran to score a try. In the last two decades in South Africa the transformation process in getting more players of colour in the national team has become a real big issue. The fast changing society since 1995, the year in which the Springboks won their first Rugby World Cup, is according to government and critics not represented in the senior national side. Change management is a business management approach to implement procedures to deal with changes and to be able to reach the desired future state by profiting from the changing opportunities. SARU wants to address these issues and changes with their Strategic Transformational Plan. The purpose of this treatise was to study the perception of the junior players on the changes in rugby and the STP. The key drivers which will have influences on change management within the sport organisation were examined by the research. With the research objectives in mind a literature review was performed to create and develop a hypothesized model, which could be tested by the design of a measuring instrument which collected quantitative data for statistical results and analysis. The interpretation of the results of the research showed that Engagement, Coaching and Mentoring, Medical and Healthcare, Education/Schooling and Culture have positive associations and influences on change management. Transformational plans, Conditioning and nutrition’s relationship with change management and influences were accepted for the null hypotheses. Finally, change management and the various issues of transformation in SA Rugby showed to be valuable, measurable to a certain extent, and its recommendations actionable. The practical significant differences between the perceptions of the race groups (White, Coloured and African) illustrated valuable information for future communications and possible studies. This study and its proposed model on change management was a benefit to all the stakeholders and sport organisation going through changes and transformation in South Africa.
- Full Text:
- Date Issued: 2015
- Authors: Erasmus, Innis
- Date: 2015
- Subjects: Organizational change -- Management , Rugby football -- South Africa , Sports administration
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: http://hdl.handle.net/10948/48819 , vital:41143
- Description: The history of the sport rugby evolved from a football game more than a century ago, in England, in a town called Rugby when a young boy by the name of William Webb Ellis picked up the ball and ran to score a try. In the last two decades in South Africa the transformation process in getting more players of colour in the national team has become a real big issue. The fast changing society since 1995, the year in which the Springboks won their first Rugby World Cup, is according to government and critics not represented in the senior national side. Change management is a business management approach to implement procedures to deal with changes and to be able to reach the desired future state by profiting from the changing opportunities. SARU wants to address these issues and changes with their Strategic Transformational Plan. The purpose of this treatise was to study the perception of the junior players on the changes in rugby and the STP. The key drivers which will have influences on change management within the sport organisation were examined by the research. With the research objectives in mind a literature review was performed to create and develop a hypothesized model, which could be tested by the design of a measuring instrument which collected quantitative data for statistical results and analysis. The interpretation of the results of the research showed that Engagement, Coaching and Mentoring, Medical and Healthcare, Education/Schooling and Culture have positive associations and influences on change management. Transformational plans, Conditioning and nutrition’s relationship with change management and influences were accepted for the null hypotheses. Finally, change management and the various issues of transformation in SA Rugby showed to be valuable, measurable to a certain extent, and its recommendations actionable. The practical significant differences between the perceptions of the race groups (White, Coloured and African) illustrated valuable information for future communications and possible studies. This study and its proposed model on change management was a benefit to all the stakeholders and sport organisation going through changes and transformation in South Africa.
- Full Text:
- Date Issued: 2015
Changes in diet resource use by elephants, Loxodonta Africana, due to changes in resource availability in the Addo National Park
- Authors: Du Toit, Jana
- Date: 2015
- Subjects: Elephants -- Nutrition -- South Africa -- Eastern Cape , Elephants -- Habitat -- South Africa -- Eastern Cape , Wildlife conservation , Addo Elephant National Park (South Africa)
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10731 , ISSN http://hdl.handle.net/10948/d1021194
- Description: Animals are restricted in their diets by several factors, most notably the availability and quality of resources. Variation in resource availability causes herbivores to shift their diets seasonally and spatially. Elephants (Loxodonta africana), are known to have extensive impacts on plant communities, altering ecosystem functioning and causing a decline in biodiversity. In enclosed areas, these impacts are increased leading to a decline in resource availability and presumably resource quality. In the Addo Elephant National Park, the Main Camp section has a history of high elephant impacts and therefore reduced resource availability. Whereas, the recently added Colchester section has greater resource availability, due to the absence of elephants in this section since the fencing of the Park. This study investigated the changes in diet (diet breadth, preference and diet quality) of elephants due to an increase in resource availability. Three alternative hypotheses were contrasted: 1) elephants as generalist foragers, 2) elephants as optimal foragers, or 3) elephants learning foraging behaviour. Using microhistological analysis, the diets of elephants were described over five sampling periods (August 2010 – February 2014) in both sections. Forage availability was estimated using a modified line-intercept method, and was used to determine changes in preference by relating forage availability to use. In the Colchester section the diet breadth of elephants increased, and was coupled with a high initial variation between the diets of elephants, which decreased in subsequent sampling periods. This supported the elephants learning foraging behaviour hypothesis. However, there was no increase in diet preference by elephants in the Colchester section, which supported the elephants as generalist foragers hypothesis. There was also no difference in the diet quality of elephants in the Main Camp and Colchester sections, which did not support any of the three hypotheses. The elephants learning foraging behaviour hypothesis is proposed to be the link between the alternate two hypotheses, and given enough time, either of the two could be supported. The lack of difference in preference and diet quality between elephants in the Main Camp and Colchester sections is hypothesised to be due to the population level (not measured for individuals) at which these were measured. Microhistological analysis of faeces was used to describe the diet of elephants, which was compared to the diet described by DNA metabarcoding. Microhistological analysis is a traditional, favoured technique used in describing the diet of wild herbivores, whereas DNA metabarcoding is a relatively new and untested technique. These two techniques have not yet been compared in the diet of megaherbivores. Results indicated that microhistological analysis identified significantly more grass in the diet of elephants, than DNA metabarcoding did, which was expected as previous studies also found overestimation of grasses. Microhistological analysis identified more plant families in the diet of elephants, than DNA metabarcoding. Most of the differences between the two techniques can be attributed to the difference in taxonomic resolution, which was due to the lack of a complete reference collection for DNA metabarcoding. Although either of the two techniques can be used to describe the diet of elephants, the most reliable results would be obtained when using both techniques. The findings of this study suggest that due to the high initial variation between the diets of elephants, with an increase in resource availability, the impacts will also initially be highly varied. This suggests that identifying plant species to monitor elephants impacts initially will be difficult. However, important plant species, or those known to be vulnerable to elephants impacts should be carefully monitored initially and monitoring should not only occur annually, but also seasonally.
- Full Text:
- Date Issued: 2015
- Authors: Du Toit, Jana
- Date: 2015
- Subjects: Elephants -- Nutrition -- South Africa -- Eastern Cape , Elephants -- Habitat -- South Africa -- Eastern Cape , Wildlife conservation , Addo Elephant National Park (South Africa)
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10731 , ISSN http://hdl.handle.net/10948/d1021194
- Description: Animals are restricted in their diets by several factors, most notably the availability and quality of resources. Variation in resource availability causes herbivores to shift their diets seasonally and spatially. Elephants (Loxodonta africana), are known to have extensive impacts on plant communities, altering ecosystem functioning and causing a decline in biodiversity. In enclosed areas, these impacts are increased leading to a decline in resource availability and presumably resource quality. In the Addo Elephant National Park, the Main Camp section has a history of high elephant impacts and therefore reduced resource availability. Whereas, the recently added Colchester section has greater resource availability, due to the absence of elephants in this section since the fencing of the Park. This study investigated the changes in diet (diet breadth, preference and diet quality) of elephants due to an increase in resource availability. Three alternative hypotheses were contrasted: 1) elephants as generalist foragers, 2) elephants as optimal foragers, or 3) elephants learning foraging behaviour. Using microhistological analysis, the diets of elephants were described over five sampling periods (August 2010 – February 2014) in both sections. Forage availability was estimated using a modified line-intercept method, and was used to determine changes in preference by relating forage availability to use. In the Colchester section the diet breadth of elephants increased, and was coupled with a high initial variation between the diets of elephants, which decreased in subsequent sampling periods. This supported the elephants learning foraging behaviour hypothesis. However, there was no increase in diet preference by elephants in the Colchester section, which supported the elephants as generalist foragers hypothesis. There was also no difference in the diet quality of elephants in the Main Camp and Colchester sections, which did not support any of the three hypotheses. The elephants learning foraging behaviour hypothesis is proposed to be the link between the alternate two hypotheses, and given enough time, either of the two could be supported. The lack of difference in preference and diet quality between elephants in the Main Camp and Colchester sections is hypothesised to be due to the population level (not measured for individuals) at which these were measured. Microhistological analysis of faeces was used to describe the diet of elephants, which was compared to the diet described by DNA metabarcoding. Microhistological analysis is a traditional, favoured technique used in describing the diet of wild herbivores, whereas DNA metabarcoding is a relatively new and untested technique. These two techniques have not yet been compared in the diet of megaherbivores. Results indicated that microhistological analysis identified significantly more grass in the diet of elephants, than DNA metabarcoding did, which was expected as previous studies also found overestimation of grasses. Microhistological analysis identified more plant families in the diet of elephants, than DNA metabarcoding. Most of the differences between the two techniques can be attributed to the difference in taxonomic resolution, which was due to the lack of a complete reference collection for DNA metabarcoding. Although either of the two techniques can be used to describe the diet of elephants, the most reliable results would be obtained when using both techniques. The findings of this study suggest that due to the high initial variation between the diets of elephants, with an increase in resource availability, the impacts will also initially be highly varied. This suggests that identifying plant species to monitor elephants impacts initially will be difficult. However, important plant species, or those known to be vulnerable to elephants impacts should be carefully monitored initially and monitoring should not only occur annually, but also seasonally.
- Full Text:
- Date Issued: 2015
Characterisation of performance limiting defects in photovoltaic devices using electroluminescence and related techniques
- Authors: Crozier, Jacqueline Louise
- Date: 2015
- Subjects: Photovoltaic cells Solar cells Photovoltaic power systems
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10948/11004 , vital:26866
- Description: Solar cells allow the energy from the sun to be converted into electrical energy; this makes solar energy an environmentally friendly, sustainable alternative to fossil fuel energy sources. Solar cells are connected together in a photovoltaic (PV) module to provide the higher current, voltage and power outputs necessary for electrical applications. However, the performance of PV modules can limited by the degradation and defects. PV modules can be characterised using various opto-electronic techniques, each providing information about the performance of the module. The current-voltage (I-V) characteristic curve of a module being the most commonly used characterisation technique. The I-V curve is typically measured in outdoor, fully illuminated, conditions. This allows performance parameters such as short circuit current (ISC), open circuit voltage (VOC) and maximum power (PMAX) to be determined. However, it can be difficult to determine the root cause of the performance drop from the I-V curve alone. Electroluminescence (EL) is a module characterisation technique that allows defects and failures in PV modules to be successfully identified. This study investigates the characterisation of solar cells and photovoltaic modules using EL. EL occurs when a solar cell or module is forward biased and the injected electron-hole pairs recombine radiatively. The intensity of the emitted EL is related the applied voltage and the material properties. EL imaging is a useful characterisation technique in identifying module defects and failures. Defects such as micro-cracks, broken contact fingers and fractures are detected in EL images as well as material features such as grain boundaries. The common defects in crystalline silicon are catalogued and the possible causes are discussed. An experimental setup was developed in order to systematically take a high resolution EL image of every cell in the module and record the applied voltage and current. This produces a very detailed, clear, image of each cell with a pixel size in the micrometre range. This process is time consuming to acquire an EL image of an entire module so alternatively a different setup can be used and an EL image of a whole module can be captured in a single frame with an increased pixel size in the millimetre range. For EL imaging a silicon charge-coupled device (CCD) camera was used because it has very good spatial resolution however this sensor is only sensitive to wavelength in the range of 300-1200 nm. There is an overlap in wavelengths from about 900 to 1100 nm allowing the EL emitted from silicon solar cells to be detected. In conjunction with the high-resolution EL system an image processing program was developed to crop, adjust and align the images so only the relevant cell was included. This program also automatically detects certain defects that have a regular shape. Micro-cracks, broken fingers and striation rings are automatically identified. The program has an adjustable sensitivity to identify small or large defects. Defective cells are distinguished from undamaged cells by comparing the binary images to the ideal, undamaged cell. The current-voltage curves and the performance parameters of modules were compared with the EL images in order to discuss and identify power limiting defects. Features that remove significant portions of the cell from electrical contact such as micro-cracks are shown to have a larger effect of the performance of the module. Other features such as broken contact fingers, contact forming failures and striation rings do not significantly lower the performance of the module. Thus an understanding of how different features affect the module performance is important in order to correctly interpret the EL results. The intensity of the luminescence emitted is related to the applied voltage and the quantum efficiency of the cell material. The spectrum of the emitted luminescence was modelled and related to the recombination properties of the cell such as surface recombination velocity and minority carrier diffusion length/lifetime. In this study the emitted spectrum was modelled and the effects of recombination properties of the cell on the emitted spectrum were examined. The spectrum of the detected EL was modelled, dependent on the sensitivity of the camera, the transmission of the filters and the emitted photon flux. The integration of short-pass filters into the experimental setup in order to isolate short-wavelength luminescence was discussed. There is a proportional relationship between the intensity of the emitted EL and the local junction voltage. Resistive losses like series and shunt resistances lower the applied voltage and thus affect the EL image. The voltage dependence was assessed by comparing EL images taken at different applied biases. Analysis of the variation in EL intensity with voltage was successful in determining the origin of certain features in an EL image. Certain defects, those that are related to series resistance or shunting are highly voltage dependent. When a feature has little or no dependence on voltage then the defect could be in the laminate layers and not in the cell material. The results of this study allow for in-depth analysis of the defects found in PV modules using the high resolution EL imaging system and the image processing routine. The development of an image processing routine allows the interpretation of the EL image to be done automatically, resulting in a faster and more efficient process. By understanding the defects visible in the EL image, the test is more meaningful and allows the results to be used to predict module performance and potential failures.
- Full Text:
- Date Issued: 2015
- Authors: Crozier, Jacqueline Louise
- Date: 2015
- Subjects: Photovoltaic cells Solar cells Photovoltaic power systems
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10948/11004 , vital:26866
- Description: Solar cells allow the energy from the sun to be converted into electrical energy; this makes solar energy an environmentally friendly, sustainable alternative to fossil fuel energy sources. Solar cells are connected together in a photovoltaic (PV) module to provide the higher current, voltage and power outputs necessary for electrical applications. However, the performance of PV modules can limited by the degradation and defects. PV modules can be characterised using various opto-electronic techniques, each providing information about the performance of the module. The current-voltage (I-V) characteristic curve of a module being the most commonly used characterisation technique. The I-V curve is typically measured in outdoor, fully illuminated, conditions. This allows performance parameters such as short circuit current (ISC), open circuit voltage (VOC) and maximum power (PMAX) to be determined. However, it can be difficult to determine the root cause of the performance drop from the I-V curve alone. Electroluminescence (EL) is a module characterisation technique that allows defects and failures in PV modules to be successfully identified. This study investigates the characterisation of solar cells and photovoltaic modules using EL. EL occurs when a solar cell or module is forward biased and the injected electron-hole pairs recombine radiatively. The intensity of the emitted EL is related the applied voltage and the material properties. EL imaging is a useful characterisation technique in identifying module defects and failures. Defects such as micro-cracks, broken contact fingers and fractures are detected in EL images as well as material features such as grain boundaries. The common defects in crystalline silicon are catalogued and the possible causes are discussed. An experimental setup was developed in order to systematically take a high resolution EL image of every cell in the module and record the applied voltage and current. This produces a very detailed, clear, image of each cell with a pixel size in the micrometre range. This process is time consuming to acquire an EL image of an entire module so alternatively a different setup can be used and an EL image of a whole module can be captured in a single frame with an increased pixel size in the millimetre range. For EL imaging a silicon charge-coupled device (CCD) camera was used because it has very good spatial resolution however this sensor is only sensitive to wavelength in the range of 300-1200 nm. There is an overlap in wavelengths from about 900 to 1100 nm allowing the EL emitted from silicon solar cells to be detected. In conjunction with the high-resolution EL system an image processing program was developed to crop, adjust and align the images so only the relevant cell was included. This program also automatically detects certain defects that have a regular shape. Micro-cracks, broken fingers and striation rings are automatically identified. The program has an adjustable sensitivity to identify small or large defects. Defective cells are distinguished from undamaged cells by comparing the binary images to the ideal, undamaged cell. The current-voltage curves and the performance parameters of modules were compared with the EL images in order to discuss and identify power limiting defects. Features that remove significant portions of the cell from electrical contact such as micro-cracks are shown to have a larger effect of the performance of the module. Other features such as broken contact fingers, contact forming failures and striation rings do not significantly lower the performance of the module. Thus an understanding of how different features affect the module performance is important in order to correctly interpret the EL results. The intensity of the luminescence emitted is related to the applied voltage and the quantum efficiency of the cell material. The spectrum of the emitted luminescence was modelled and related to the recombination properties of the cell such as surface recombination velocity and minority carrier diffusion length/lifetime. In this study the emitted spectrum was modelled and the effects of recombination properties of the cell on the emitted spectrum were examined. The spectrum of the detected EL was modelled, dependent on the sensitivity of the camera, the transmission of the filters and the emitted photon flux. The integration of short-pass filters into the experimental setup in order to isolate short-wavelength luminescence was discussed. There is a proportional relationship between the intensity of the emitted EL and the local junction voltage. Resistive losses like series and shunt resistances lower the applied voltage and thus affect the EL image. The voltage dependence was assessed by comparing EL images taken at different applied biases. Analysis of the variation in EL intensity with voltage was successful in determining the origin of certain features in an EL image. Certain defects, those that are related to series resistance or shunting are highly voltage dependent. When a feature has little or no dependence on voltage then the defect could be in the laminate layers and not in the cell material. The results of this study allow for in-depth analysis of the defects found in PV modules using the high resolution EL imaging system and the image processing routine. The development of an image processing routine allows the interpretation of the EL image to be done automatically, resulting in a faster and more efficient process. By understanding the defects visible in the EL image, the test is more meaningful and allows the results to be used to predict module performance and potential failures.
- Full Text:
- Date Issued: 2015
Characterisation of the HSP70-HSP90 organising protein gene and its link to cancer
- Authors: Weeks, Stacey
- Date: 2015
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/56006 , vital:26764
- Description: HOP (Heat shock protein 70/ Heat shock protein 90 organising protein) is a co-chaperone essential for client protein transfer from HSP70 to HSP90 within the HSP90 chaperone machine and has been found to be up-regulated in various cancers. However, minimal in vitro information can be found on the regulation of HOP expression. The aim of this study was to analyse the HOP gene structure across known orthologues, identify and characterise the HOP promoter, and identify the regulatory mechanisms influencing the expression of HOP in cancer. We hypothesized that the expression of HOP in cancer cells is likely regulated by oncogenic signalling pathways linked to cis-elements within the HOP promoter. An initial study of the evolution of the HOP gene speciation was performed across identified orthologues using Mega5.2. The evolutionary pathway of the HOP gene was traced from the unicellular organisms to fish, to amphibian and then to land mammal. The synteny across the orthologues was identified and the co-expression profile of HOP analysed. We identified the putative promoter region for HOP in silico and in vitro. Luciferase reporter assays were utilized to demonstrate promoter activity of the upstream region in vitro. Bioinformatic analysis of the active promoter region identified a large CpG island and a range of putative cis-elements. Many of the cis-elements interact with transcription factors which are activated by oncogenic pathways. We therefore tested the regulation of HOP levels by rat sarcoma viral oncogene homologue (RAS). Cancer cell lines were transfected with mutated RAS to observe the effect of constitutively active RAS expression on the production of HOP using qRT-PCR and Western Blot analyses. Additionally, inhibitors of the RAS signalling pathway were utilised to confirm the regulatory effect of mutated RAS on HOP expression. In cancer cell lines containing mutated RAS (Hs578T), HOP was up-regulated via a mechanism involving the MAPK signalling pathway and the ETS-1 and C/EBPβ cis-elements within the HOP promoter. These findings suggest for the first time that Hop expression in cancer may be regulated by RAS activation of the HOP promoter. Additionally, this study allowed us to determine the murine system to be the most suited genetic model organism with which to study the function of human HOP.
- Full Text:
- Date Issued: 2015
- Authors: Weeks, Stacey
- Date: 2015
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/56006 , vital:26764
- Description: HOP (Heat shock protein 70/ Heat shock protein 90 organising protein) is a co-chaperone essential for client protein transfer from HSP70 to HSP90 within the HSP90 chaperone machine and has been found to be up-regulated in various cancers. However, minimal in vitro information can be found on the regulation of HOP expression. The aim of this study was to analyse the HOP gene structure across known orthologues, identify and characterise the HOP promoter, and identify the regulatory mechanisms influencing the expression of HOP in cancer. We hypothesized that the expression of HOP in cancer cells is likely regulated by oncogenic signalling pathways linked to cis-elements within the HOP promoter. An initial study of the evolution of the HOP gene speciation was performed across identified orthologues using Mega5.2. The evolutionary pathway of the HOP gene was traced from the unicellular organisms to fish, to amphibian and then to land mammal. The synteny across the orthologues was identified and the co-expression profile of HOP analysed. We identified the putative promoter region for HOP in silico and in vitro. Luciferase reporter assays were utilized to demonstrate promoter activity of the upstream region in vitro. Bioinformatic analysis of the active promoter region identified a large CpG island and a range of putative cis-elements. Many of the cis-elements interact with transcription factors which are activated by oncogenic pathways. We therefore tested the regulation of HOP levels by rat sarcoma viral oncogene homologue (RAS). Cancer cell lines were transfected with mutated RAS to observe the effect of constitutively active RAS expression on the production of HOP using qRT-PCR and Western Blot analyses. Additionally, inhibitors of the RAS signalling pathway were utilised to confirm the regulatory effect of mutated RAS on HOP expression. In cancer cell lines containing mutated RAS (Hs578T), HOP was up-regulated via a mechanism involving the MAPK signalling pathway and the ETS-1 and C/EBPβ cis-elements within the HOP promoter. These findings suggest for the first time that Hop expression in cancer may be regulated by RAS activation of the HOP promoter. Additionally, this study allowed us to determine the murine system to be the most suited genetic model organism with which to study the function of human HOP.
- Full Text:
- Date Issued: 2015
Characterization of polychlorinated biphenyl residues in the North End lake and Port Elizabeth harbour, South Africa
- Authors: Kampire, Edwige
- Date: 2015
- Subjects: Marine pollution -- South Africa -- Port Elizabeth , Estuarine pollution -- South Africa -- Port Elizabeth
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10948/7903 , vital:24320
- Description: Persistent organic pollutants (POPs), including polychlorinated biphenyls (PCBs) are widespread in aquatic systems, they can bioaccumulate in the tissues of aquatic organisms, especially fish as they occupy a position near the top of the aquatic food web. PCBs are among the most toxic substances and have been shown to cause many adverse effects to humans and wildlife. High health risks are associated with populations that follow a diet high in fat content such as fish and shellfish in which PCBs bioaccumulate. Given the importance of industries and the potential health concerns of exposure to PCBs, the lack of information on environmental levels of PCBs in South Africa (SA) is significant and concerning. Less attention has been directed to analysis of PCBs in the South African environments due to high cost and lack of appropriate equipment. This study was the first conducted in order to assess the levels of PCBs in the environment of Nelson Mandela Bay Municipality (Port Elizabeth). The research was directed at determining the PCB levels in water, sediments, fish and mussels collected in the Nelson Mandela Bay Municipality. The sampling sites, North End Lake (NEL) and Port Elizabeth Harbour (PEH) were selected based on their location and the importance of activities taking place in these areas. In total 456 samples of water (dissolved and particulate phases), sediments, mussels and tissues of fish were analysed. Suitable analytical methods were based on the equipment and materials available at the Nelson Mandela Metropolitan University. Gas chromatography-mass spectrometry (GC/MS) was used to quantify PCBs in the samples using the internal standard method. Six indicator congeners (PCB nos. 28, 52, 101, 138, 153, 180) were chosen because they are mainly present in most PCB mixtures in environmental samples and they are recommended for regular monitoring. These congeners represent about 50% of the total non-dioxin like (NDL) PCBs in food. All these congeners were detected in the samples analysed. The total PCB concentrations in the sediments from the NEL and PEH ranged from 1.60 to 3.06 and from 0.56 to 2.35 ng g-1 dry weight, respectively. The concentrations of total PCBs in Mytilus galloprovincialis ranged from 14.48 to 21.37 ng g-1 wet weight and from 20.84 to 31.34 ng g-1 wet weight in Perna perna. Total PCBs in water ranged from 0.18 to 0.355 ng L-1. The concentrations of total PCBs in the liver, gonads, gills and muscle were 95.69, 57.49, 44.63, 34.14 ng g-1 lipid weight in Cyprinus carpio and 119.73, 59.21, 49.78, 34.63 ng g-1 in Oreochromis mossambicus, respectively. Fish liver was the most contaminated organ due to its high lipid content compared to other parts of fish analysed. The NEL was found to be more contaminated than the PEH. The main potential sources of PCB pollutants in both areas of this study are industrial and municipal discharges. The NEL is surrounded by many industries and serves as a potential pollutant sink due to wastewater and inflow entering into this lake. Four 0.227 kg meals of the edible part of fish (muscle) per month were recommended based on the non-cancer health endpoint and one 0.227 kg meal per month was recommended based on the cancer health endpoint. This research contributed to notify the public and relevant governmental departments on the PCB pollution status of sediments, water and aquatic life in the PEH and NEL.
- Full Text:
- Date Issued: 2015
- Authors: Kampire, Edwige
- Date: 2015
- Subjects: Marine pollution -- South Africa -- Port Elizabeth , Estuarine pollution -- South Africa -- Port Elizabeth
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10948/7903 , vital:24320
- Description: Persistent organic pollutants (POPs), including polychlorinated biphenyls (PCBs) are widespread in aquatic systems, they can bioaccumulate in the tissues of aquatic organisms, especially fish as they occupy a position near the top of the aquatic food web. PCBs are among the most toxic substances and have been shown to cause many adverse effects to humans and wildlife. High health risks are associated with populations that follow a diet high in fat content such as fish and shellfish in which PCBs bioaccumulate. Given the importance of industries and the potential health concerns of exposure to PCBs, the lack of information on environmental levels of PCBs in South Africa (SA) is significant and concerning. Less attention has been directed to analysis of PCBs in the South African environments due to high cost and lack of appropriate equipment. This study was the first conducted in order to assess the levels of PCBs in the environment of Nelson Mandela Bay Municipality (Port Elizabeth). The research was directed at determining the PCB levels in water, sediments, fish and mussels collected in the Nelson Mandela Bay Municipality. The sampling sites, North End Lake (NEL) and Port Elizabeth Harbour (PEH) were selected based on their location and the importance of activities taking place in these areas. In total 456 samples of water (dissolved and particulate phases), sediments, mussels and tissues of fish were analysed. Suitable analytical methods were based on the equipment and materials available at the Nelson Mandela Metropolitan University. Gas chromatography-mass spectrometry (GC/MS) was used to quantify PCBs in the samples using the internal standard method. Six indicator congeners (PCB nos. 28, 52, 101, 138, 153, 180) were chosen because they are mainly present in most PCB mixtures in environmental samples and they are recommended for regular monitoring. These congeners represent about 50% of the total non-dioxin like (NDL) PCBs in food. All these congeners were detected in the samples analysed. The total PCB concentrations in the sediments from the NEL and PEH ranged from 1.60 to 3.06 and from 0.56 to 2.35 ng g-1 dry weight, respectively. The concentrations of total PCBs in Mytilus galloprovincialis ranged from 14.48 to 21.37 ng g-1 wet weight and from 20.84 to 31.34 ng g-1 wet weight in Perna perna. Total PCBs in water ranged from 0.18 to 0.355 ng L-1. The concentrations of total PCBs in the liver, gonads, gills and muscle were 95.69, 57.49, 44.63, 34.14 ng g-1 lipid weight in Cyprinus carpio and 119.73, 59.21, 49.78, 34.63 ng g-1 in Oreochromis mossambicus, respectively. Fish liver was the most contaminated organ due to its high lipid content compared to other parts of fish analysed. The NEL was found to be more contaminated than the PEH. The main potential sources of PCB pollutants in both areas of this study are industrial and municipal discharges. The NEL is surrounded by many industries and serves as a potential pollutant sink due to wastewater and inflow entering into this lake. Four 0.227 kg meals of the edible part of fish (muscle) per month were recommended based on the non-cancer health endpoint and one 0.227 kg meal per month was recommended based on the cancer health endpoint. This research contributed to notify the public and relevant governmental departments on the PCB pollution status of sediments, water and aquatic life in the PEH and NEL.
- Full Text:
- Date Issued: 2015
Characterization of some virulence and antibiotic resistance genes of Staphylococcus aureus isolated from cases of Bovine Mastitis in Nkonkobe Municipality, Eastern Cape Province, RSA
- Authors: Pekana, Abongile
- Date: 2015
- Language: English
- Type: Thesis , Masters , MSc (Microbiology)
- Identifier: vital:11293 , http://hdl.handle.net/10353/d1021133
- Description: Staphylococcus aureus is one of the predominant causative agents of mastitis disease in dairy herds. Mastitis disease has a negative impact in the economic losses in the dairy sector across the globe. The aim of this study is to detect some of the virulence genes in the S. aureus isolated from 400 milk samples of subclinical and clinical mastitis dairy cows in Fort Hare dairy farm and Middle Drift dairy farm in Alice in the Eastern Cape province of South Africa. In addition antibiotic resistance pattern and antibiotic resistance genes were investigated. Gram-staining, oxidase test, catalase test and API Staph kit were preliminary biochemical tests used for the identification of S. aureus isolates. The MALDI-TOF-MS was also used for further identification. Polymerase chain reaction was performed of genes encoding antibiotic resistance as well as clumping (clfA), coagulase (coa) gene, toxic shock syndrome (tsst), exfoliative toxin A and B (eta and etb), and the gene segment encoding the immunoglobulin G binding region and X region of protein gene spa. A total of 20 (5%) S. aureus strains obtained from 400 milk samples from the two farms were subjected to 16 antibiotics for antibiotic susceptibility testing. In Middle Drift dairy farm 11 (5.5%) isolates were obtained from 200 samples and 9 (4.5%) isolates were obtained in Fort Hare dairy farm from 200 samples. A large percent of the isolates were resistant to penicillin G (60%), followed by trimethoprim (60%) and tetracycline (60%), trimethoprim-sulfamethaxazole (55%), telithroprim (55%) and doxycycline (45%). Most of the isolates were sensitive to several (50-85%) antibiotics. Of the twenty isolates tested 12 samples contained the penicillin antibiotic resistance gene (blaZ gene), 8 samples contained at least one aminoglycoside-modifying enzyme gene (AME gene); the (aac(6’)/aph(2’’) gene and no amplification occurred for aph(3’)-IIIa and ant(4’)-Ia) genes. In the case of the tetracycline antibiotic resistance gene (tetK and tetM), 2 samples contained tetM and a single sample contained tetK gene. No amplification was observed for the erythromycin antibiotic resistance genes (ermA, ermB, ermC, Mef and msrA). All the samples tested were negative for the expression of toxic syndrome gene (tsst), etb, and Immunoglobulin G binding region. However, amplification of the clumping factor was observed in 7 (35%) isolates of S. aureus, exfoliative toxin (eta) expressed 4(20%) isolates; coagulase gene (coa) yielded six DNA bands of six differences sizes from 16 (80%) isolates. A total of four different bands size were expressed for the spa X region from 12 (60%) isolates. The data obtained in this study suggests that poor hygienic practices and inadequate management practices are responsible for the increase in Staphylococcus aureus isolation. The high resistance of S. aureus to antibiotics and the distribution of virulence genes contribute in bovine mastitis in these farms may cause health problems in the community consuming raw milk purchased from these farms.
- Full Text:
- Date Issued: 2015
- Authors: Pekana, Abongile
- Date: 2015
- Language: English
- Type: Thesis , Masters , MSc (Microbiology)
- Identifier: vital:11293 , http://hdl.handle.net/10353/d1021133
- Description: Staphylococcus aureus is one of the predominant causative agents of mastitis disease in dairy herds. Mastitis disease has a negative impact in the economic losses in the dairy sector across the globe. The aim of this study is to detect some of the virulence genes in the S. aureus isolated from 400 milk samples of subclinical and clinical mastitis dairy cows in Fort Hare dairy farm and Middle Drift dairy farm in Alice in the Eastern Cape province of South Africa. In addition antibiotic resistance pattern and antibiotic resistance genes were investigated. Gram-staining, oxidase test, catalase test and API Staph kit were preliminary biochemical tests used for the identification of S. aureus isolates. The MALDI-TOF-MS was also used for further identification. Polymerase chain reaction was performed of genes encoding antibiotic resistance as well as clumping (clfA), coagulase (coa) gene, toxic shock syndrome (tsst), exfoliative toxin A and B (eta and etb), and the gene segment encoding the immunoglobulin G binding region and X region of protein gene spa. A total of 20 (5%) S. aureus strains obtained from 400 milk samples from the two farms were subjected to 16 antibiotics for antibiotic susceptibility testing. In Middle Drift dairy farm 11 (5.5%) isolates were obtained from 200 samples and 9 (4.5%) isolates were obtained in Fort Hare dairy farm from 200 samples. A large percent of the isolates were resistant to penicillin G (60%), followed by trimethoprim (60%) and tetracycline (60%), trimethoprim-sulfamethaxazole (55%), telithroprim (55%) and doxycycline (45%). Most of the isolates were sensitive to several (50-85%) antibiotics. Of the twenty isolates tested 12 samples contained the penicillin antibiotic resistance gene (blaZ gene), 8 samples contained at least one aminoglycoside-modifying enzyme gene (AME gene); the (aac(6’)/aph(2’’) gene and no amplification occurred for aph(3’)-IIIa and ant(4’)-Ia) genes. In the case of the tetracycline antibiotic resistance gene (tetK and tetM), 2 samples contained tetM and a single sample contained tetK gene. No amplification was observed for the erythromycin antibiotic resistance genes (ermA, ermB, ermC, Mef and msrA). All the samples tested were negative for the expression of toxic syndrome gene (tsst), etb, and Immunoglobulin G binding region. However, amplification of the clumping factor was observed in 7 (35%) isolates of S. aureus, exfoliative toxin (eta) expressed 4(20%) isolates; coagulase gene (coa) yielded six DNA bands of six differences sizes from 16 (80%) isolates. A total of four different bands size were expressed for the spa X region from 12 (60%) isolates. The data obtained in this study suggests that poor hygienic practices and inadequate management practices are responsible for the increase in Staphylococcus aureus isolation. The high resistance of S. aureus to antibiotics and the distribution of virulence genes contribute in bovine mastitis in these farms may cause health problems in the community consuming raw milk purchased from these farms.
- Full Text:
- Date Issued: 2015
Characterization of the co-chaperones of Hsp70 and Hsp90 in Trypanosoma brucei and their potential partnerships
- Authors: Mokoena, Fortunate
- Date: 2015
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/54543 , vital:26583
- Description: African Trypanosomiasis, which is caused by Trypanosoma brucei, is one of the crippling agents of social and economic development in Africa. T. brucei cycles between the cold-blooded insect vector, the tsetse fly (Glossina spp), and warm-blooded mammalian hosts. T. brucei, T. cruzi and L. major are mammal infecting kinetoplastid parasites that are collectively referred to as TriTryps. These parasites experience extreme environments as they move between their warm-blooded mammalian hosts and cold-blooded insect vectors which trigger extensive morphological transformations during the life-cycle of the parasite. Molecular chaperones have been implicated in parasite differentiation. TriTryps display significant expansions and diversity in the gene complements encoding molecular chaperones, especially J-proteins. Generally, J-proteins function as co-chaperones of Hsp70s, forming part of vital protein homeostasis processes. Hsp70s show a high degree of conservation, while J-proteins appear to be an extreme case of taxonomic radiation. Although several studies have focused on the molecular and cell biology of Hsp70s in some kinetoplastid parasites, knowledge is still lacking pertaining to J-proteins and their partnerships with Hsp70s. This thesis focused on the classification of kinetoplastid Jproteins into the four types by examining the domain organizations using T. brucei as a guide. The potential partnership of J-proteins and Hsp70s were postulated based on predicted subcellular localization. Kinetoplastid parasites, particularly T. brucei, have evolved an expanded and specialized J-protein machinery, likely to be a consequence of an evolutionary fitness/trait to adapt to diverse environment present in hosts and vectors. These analyses will yield insight into the process of parasite differentiation as well as provide new leads for chemotherapeutic treatments. The presence of the STI1 mediated Hsp90 hetero-complex formation has not been confirmed in T. brucei. To this end, in silico and biochemical techniques were used to characterize the role of TbSTI1, as an adaptor protein of Hsp70 and Hsp90. Through domain architecture analysis, sequence alignments, phylogenetic analysis and three-dimensional structure prediction, TbSTI1 was demonstrated to be the most conserved TPR containing co-chaperone of Hsp70 and Hsp83 in T. brucei and also shown to be highly similar to its eukaryotic homologues. Recombinant TbSTI1 was overproduced and purified in E.coli cells and subsequently shown to associate with TcHsp70 in a concentration dependent manner and associate weakly with TbHsp70.4. TbSTI1 and TbHsp83 were also demonstrated to be expressed and upregulated upon exposure to heat shock at the bloodstream stage of parasite development. In conclusion, this study is the first to report the interaction of TbSTI1 with a chaperone. Interactions between TbSTI1 and Hsp70s were demonstrated and therefore, the formation of the hetero-complex is predicted based the similarity of TbSTI1 to other STI1 proteins.
- Full Text:
- Date Issued: 2015
- Authors: Mokoena, Fortunate
- Date: 2015
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/54543 , vital:26583
- Description: African Trypanosomiasis, which is caused by Trypanosoma brucei, is one of the crippling agents of social and economic development in Africa. T. brucei cycles between the cold-blooded insect vector, the tsetse fly (Glossina spp), and warm-blooded mammalian hosts. T. brucei, T. cruzi and L. major are mammal infecting kinetoplastid parasites that are collectively referred to as TriTryps. These parasites experience extreme environments as they move between their warm-blooded mammalian hosts and cold-blooded insect vectors which trigger extensive morphological transformations during the life-cycle of the parasite. Molecular chaperones have been implicated in parasite differentiation. TriTryps display significant expansions and diversity in the gene complements encoding molecular chaperones, especially J-proteins. Generally, J-proteins function as co-chaperones of Hsp70s, forming part of vital protein homeostasis processes. Hsp70s show a high degree of conservation, while J-proteins appear to be an extreme case of taxonomic radiation. Although several studies have focused on the molecular and cell biology of Hsp70s in some kinetoplastid parasites, knowledge is still lacking pertaining to J-proteins and their partnerships with Hsp70s. This thesis focused on the classification of kinetoplastid Jproteins into the four types by examining the domain organizations using T. brucei as a guide. The potential partnership of J-proteins and Hsp70s were postulated based on predicted subcellular localization. Kinetoplastid parasites, particularly T. brucei, have evolved an expanded and specialized J-protein machinery, likely to be a consequence of an evolutionary fitness/trait to adapt to diverse environment present in hosts and vectors. These analyses will yield insight into the process of parasite differentiation as well as provide new leads for chemotherapeutic treatments. The presence of the STI1 mediated Hsp90 hetero-complex formation has not been confirmed in T. brucei. To this end, in silico and biochemical techniques were used to characterize the role of TbSTI1, as an adaptor protein of Hsp70 and Hsp90. Through domain architecture analysis, sequence alignments, phylogenetic analysis and three-dimensional structure prediction, TbSTI1 was demonstrated to be the most conserved TPR containing co-chaperone of Hsp70 and Hsp83 in T. brucei and also shown to be highly similar to its eukaryotic homologues. Recombinant TbSTI1 was overproduced and purified in E.coli cells and subsequently shown to associate with TcHsp70 in a concentration dependent manner and associate weakly with TbHsp70.4. TbSTI1 and TbHsp83 were also demonstrated to be expressed and upregulated upon exposure to heat shock at the bloodstream stage of parasite development. In conclusion, this study is the first to report the interaction of TbSTI1 with a chaperone. Interactions between TbSTI1 and Hsp70s were demonstrated and therefore, the formation of the hetero-complex is predicted based the similarity of TbSTI1 to other STI1 proteins.
- Full Text:
- Date Issued: 2015