In-silico investigation of the effects of genetic mutations on the structural dynamics of thiopurine s-methyltransferase and their implications on the metabolism of 6-mercaptopurine
- Authors: Mwaniki, Rehema Mukami
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/432553 , vital:72880
- Description: Thiopurine S-methyltransferase (TPMT) is a cytosolic enzyme that catalyzes the S-methylation of aromatic and heterocyclic sulfhydryl compounds such as 6-mercaptopurine (6MP), 6-thioguanine (6TG) and azathioprine (AZA) which is first converted to 6MP through reduction by glutathione S- transferases (GST). The compounds, generally referred to as thiopurines, are immunosuppressants used to treat childhood acute lymphoblastic leukemia (ALL), autoimmune disorders and transplant rejection. Thiopurines are prodrugs which require metabolic activation to give thioguanine nucleotides that exert their cytotoxic effects by incorporation into DNA or inhibiting purine synthesis. The methylation reaction by TPMT utilizing S-adenosylmethionine (SAM) as the methyl donor prevents their conversion to these toxic compounds. The catalytic activity of TPMT in metabolising these compounds has been associated with occurrence of genetic variations. The variations that result to missense mutations cause amino-acid changes and in turn alter the polypeptide sequence of the protein. This could alter functionality and structural dynamics of the enzyme. This study sought to understand the underlying mechanism by which 7 specially selected mutations impede metabolic activity of the enzyme on 6-MP using in silico techniques. VAPOR and PredictSNP were used to predict the effects of single nucleotide polymorphisms (SNPs) on the stability and function of the enzyme. Of the 7 mutations, only H227Q was predicted to be functionally benign while the rest (L49S, L69V, A80P, R163H, R163C and R163P) were predicted to be deleterious or associated with disease. All the SNPs were predicted to destabilize the enzyme. Molecular dynamics (MD) simulations were preformed to mimic the behaviour of the apo, holo and drug-bound WT and mutant enzymes in vivo. This was followed by post-MD analysis to identify changes in the local and global motions of the protein in the presence of mutations and changes in intra-protein communication networks through contact map and centrality metrics calculations. RMSD and Rg analyses were performed to assess changes in global motions and compactness of the enzyme in the apo, holo and drug-bound states and in the presence of mutations. These revealed that binding of the ligand had a stabilizing effect on the WT enzyme evident from more steady trends from the analyses across trajectories in the holo and drug-bound enzymes compared to the apoenzyme. The occurrence of mutations had an effect on the global motions and compactness of the enzyme across the trajectories. Most mutations resulted in destabilized systems and less compact structures shown by unsteady RMSD and Rg across trajectories respectively. The drugbound systems appeared to be more stable in most of the systems meaning that the binding of 6MP stabilized the enzyme regardless of the presence of a mutation. RMSF analysis recorded local changes in residue flexibility due to the presence of mutations in all the systems. All the drug-bound mutant systems lost flexibility on the αAhelix which caps the active site. This could have an effect on drug binding and result to defective drug metabolism. The A80P mutation resulted to a more rigid structure from both global and local motions compared to the WT enzyme which could be associated with its nearly loss of function in vivo and in vitro. Dynamic cross correlation calculations were performed to assess how the atoms moved together. Correlated, anti-correlated and areas of no correlations were recorded in all the systems and in similar places when compared to each other. This meant that occurrence of mutations had no effect on how the atoms moved together. Contact map analysis showed that occurrence of mutations caused changes in interactions around the positions where the mutations occurred, which could have an effect on protein structural dynamics. The A80P substitution which occurred on the surface away from the binding site was identified as an allosteric mutation that resulted to changes in the catalytic site. Contact maps for the drug-cofactor complex in the mutant systems in comparison with the WT protein revealed changes that could suggest reorientation of the drug at the catalytic site. This could be an implication to altered drug metabolism. Eigenvector centrality (EC) and betweenness centrality (BC) for the most equilibrated portions of the trajectories were calculated for all the studied systems to identify residues connected to the most important residues and those that were spanned the most in shortest paths connecting other residues. Areas that scored highest in these metrics where mostly found in regions surrounding the catalytic site. Top 5% centrality hubs calculations showed loss of major hubs due to mutations with gaining of new ones. This means that mutations affected communication networks within the protein. The gained hubs were in areas close-by the lost ones which could have been an attempt of the protein to accommodate the mutations. Persistent top 5% BC hubs were identified at positions 90 and 151 while one persistent top 5% EC hub was identified at position 70. This positions play important roles in shaping the catalytic site and are in direct contact with the ligands. It was concluded that in silico techniques and analysis applied in this study revealed possible mechanisms in which genetic variations affected the structural dynamics of TMPT enzyme an affecte 6MP metabolism. , Thesis (MSc) -- Faculty of Science, Biochemistry and Microbiology, 2023
- Full Text:
- Date Issued: 2023-10-13
- Authors: Mwaniki, Rehema Mukami
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/432553 , vital:72880
- Description: Thiopurine S-methyltransferase (TPMT) is a cytosolic enzyme that catalyzes the S-methylation of aromatic and heterocyclic sulfhydryl compounds such as 6-mercaptopurine (6MP), 6-thioguanine (6TG) and azathioprine (AZA) which is first converted to 6MP through reduction by glutathione S- transferases (GST). The compounds, generally referred to as thiopurines, are immunosuppressants used to treat childhood acute lymphoblastic leukemia (ALL), autoimmune disorders and transplant rejection. Thiopurines are prodrugs which require metabolic activation to give thioguanine nucleotides that exert their cytotoxic effects by incorporation into DNA or inhibiting purine synthesis. The methylation reaction by TPMT utilizing S-adenosylmethionine (SAM) as the methyl donor prevents their conversion to these toxic compounds. The catalytic activity of TPMT in metabolising these compounds has been associated with occurrence of genetic variations. The variations that result to missense mutations cause amino-acid changes and in turn alter the polypeptide sequence of the protein. This could alter functionality and structural dynamics of the enzyme. This study sought to understand the underlying mechanism by which 7 specially selected mutations impede metabolic activity of the enzyme on 6-MP using in silico techniques. VAPOR and PredictSNP were used to predict the effects of single nucleotide polymorphisms (SNPs) on the stability and function of the enzyme. Of the 7 mutations, only H227Q was predicted to be functionally benign while the rest (L49S, L69V, A80P, R163H, R163C and R163P) were predicted to be deleterious or associated with disease. All the SNPs were predicted to destabilize the enzyme. Molecular dynamics (MD) simulations were preformed to mimic the behaviour of the apo, holo and drug-bound WT and mutant enzymes in vivo. This was followed by post-MD analysis to identify changes in the local and global motions of the protein in the presence of mutations and changes in intra-protein communication networks through contact map and centrality metrics calculations. RMSD and Rg analyses were performed to assess changes in global motions and compactness of the enzyme in the apo, holo and drug-bound states and in the presence of mutations. These revealed that binding of the ligand had a stabilizing effect on the WT enzyme evident from more steady trends from the analyses across trajectories in the holo and drug-bound enzymes compared to the apoenzyme. The occurrence of mutations had an effect on the global motions and compactness of the enzyme across the trajectories. Most mutations resulted in destabilized systems and less compact structures shown by unsteady RMSD and Rg across trajectories respectively. The drugbound systems appeared to be more stable in most of the systems meaning that the binding of 6MP stabilized the enzyme regardless of the presence of a mutation. RMSF analysis recorded local changes in residue flexibility due to the presence of mutations in all the systems. All the drug-bound mutant systems lost flexibility on the αAhelix which caps the active site. This could have an effect on drug binding and result to defective drug metabolism. The A80P mutation resulted to a more rigid structure from both global and local motions compared to the WT enzyme which could be associated with its nearly loss of function in vivo and in vitro. Dynamic cross correlation calculations were performed to assess how the atoms moved together. Correlated, anti-correlated and areas of no correlations were recorded in all the systems and in similar places when compared to each other. This meant that occurrence of mutations had no effect on how the atoms moved together. Contact map analysis showed that occurrence of mutations caused changes in interactions around the positions where the mutations occurred, which could have an effect on protein structural dynamics. The A80P substitution which occurred on the surface away from the binding site was identified as an allosteric mutation that resulted to changes in the catalytic site. Contact maps for the drug-cofactor complex in the mutant systems in comparison with the WT protein revealed changes that could suggest reorientation of the drug at the catalytic site. This could be an implication to altered drug metabolism. Eigenvector centrality (EC) and betweenness centrality (BC) for the most equilibrated portions of the trajectories were calculated for all the studied systems to identify residues connected to the most important residues and those that were spanned the most in shortest paths connecting other residues. Areas that scored highest in these metrics where mostly found in regions surrounding the catalytic site. Top 5% centrality hubs calculations showed loss of major hubs due to mutations with gaining of new ones. This means that mutations affected communication networks within the protein. The gained hubs were in areas close-by the lost ones which could have been an attempt of the protein to accommodate the mutations. Persistent top 5% BC hubs were identified at positions 90 and 151 while one persistent top 5% EC hub was identified at position 70. This positions play important roles in shaping the catalytic site and are in direct contact with the ligands. It was concluded that in silico techniques and analysis applied in this study revealed possible mechanisms in which genetic variations affected the structural dynamics of TMPT enzyme an affecte 6MP metabolism. , Thesis (MSc) -- Faculty of Science, Biochemistry and Microbiology, 2023
- Full Text:
- Date Issued: 2023-10-13
International students’ experiences of othering in tertiary education in South Africa: an exploratory study
- Authors: Lobakeng, Tsholofelo
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/424986 , vital:72199
- Description: Despite rising cases of xenophobia in South Africa, international students remain committed to enrolling in its universities. Surprisingly, there is limited research looking into xenophobia in tertiary institutions. Therefore, the present study aimed to contribute to the scarcity of knowledge on the experiences of international students in South Africa. Thus, the study explored experiences of Othering among international students in tertiary education in South Africa and how they make sense of these experiences. Purposive and snowballing sampling techniques were employed to recruit international students (N=5) from Botswana, China, Zambia, and Zimbabwe. Data was collected through semi-structured, face-to-face online interviews. Participants reported experiencing a variety of non-violent forms of Othering, such as feeling excluded due to the intentional use of local languages in both classroom and social settings; being rejected by local students who refused to work with them; and being accused of stealing opportunities from local students. At an institutional level, participants reported systemic Othering rooted in unsupportive policies and practices that led to a negative and oppressive environment. The findings support previous research studies that purport the Othering of international students in higher education in South Africa mainly manifests through non-violent and covert forms. Moreover, participants attributed Othering of immigrants to racism, a perceived lack of exposure to diversity among locals, perceived lack of compassion for immigrants, and the tendency to scapegoat immigrants for the lack of employment opportunities in the country. The findings underscore the intricate nature of Othering and the need for further research to gain a greater understanding of this complex phenomenon. Furthermore, the findings highlight the importance of implementing effective strategies of inclusion and integration of international students at these institutions of higher learning. , Thesis (MA) -- Faculty of Humanities, Psychology, 2023
- Full Text:
- Date Issued: 2023-10-13
- Authors: Lobakeng, Tsholofelo
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/424986 , vital:72199
- Description: Despite rising cases of xenophobia in South Africa, international students remain committed to enrolling in its universities. Surprisingly, there is limited research looking into xenophobia in tertiary institutions. Therefore, the present study aimed to contribute to the scarcity of knowledge on the experiences of international students in South Africa. Thus, the study explored experiences of Othering among international students in tertiary education in South Africa and how they make sense of these experiences. Purposive and snowballing sampling techniques were employed to recruit international students (N=5) from Botswana, China, Zambia, and Zimbabwe. Data was collected through semi-structured, face-to-face online interviews. Participants reported experiencing a variety of non-violent forms of Othering, such as feeling excluded due to the intentional use of local languages in both classroom and social settings; being rejected by local students who refused to work with them; and being accused of stealing opportunities from local students. At an institutional level, participants reported systemic Othering rooted in unsupportive policies and practices that led to a negative and oppressive environment. The findings support previous research studies that purport the Othering of international students in higher education in South Africa mainly manifests through non-violent and covert forms. Moreover, participants attributed Othering of immigrants to racism, a perceived lack of exposure to diversity among locals, perceived lack of compassion for immigrants, and the tendency to scapegoat immigrants for the lack of employment opportunities in the country. The findings underscore the intricate nature of Othering and the need for further research to gain a greater understanding of this complex phenomenon. Furthermore, the findings highlight the importance of implementing effective strategies of inclusion and integration of international students at these institutions of higher learning. , Thesis (MA) -- Faculty of Humanities, Psychology, 2023
- Full Text:
- Date Issued: 2023-10-13
Investigating pedagogical practices of English First Additional Language educators in Grade 12 short stories: a case study
- Authors: Jabe, Sizwe
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/424052 , vital:72119
- Description: The focus of this study was to investigate pedagogical practices of English First Additional Language educators in teaching Grade 12 short stories. An interpretive qualitative case study of three educators from three high schools in Chris Hani West district, in Eastern Cape were purposefully selected. Lesson observations, semi-structured interviews, and document analysis (lesson plans) with three English FAL educators were used to collect data. Shulman’s (1986) Pedagogical Content Knowledge was used as the theoretical framework and analytical tool for the study. Data revealed ineffective teaching strategies and on-the-surface subject matter knowledge that hampers the critical teaching of short stories. It also showed that educators have limited knowledge of learners’ learning difficulties and how to address them. The study recommends less teacher talk that was observed in many classes, to be replaced by extended discussions on the part of the learners. Educators should establish a variety of contemporary teaching strategies which put critical thinking and active learning before examination preparation. Lastly, educators, in their teaching of short stories, should consult various resources and attend content workshops to keep subject knowledge up to date. , Thesis (MEd) -- Faculty of Humanities, Study of Englishes of Africa, 2023
- Full Text:
- Date Issued: 2023-10-13
- Authors: Jabe, Sizwe
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/424052 , vital:72119
- Description: The focus of this study was to investigate pedagogical practices of English First Additional Language educators in teaching Grade 12 short stories. An interpretive qualitative case study of three educators from three high schools in Chris Hani West district, in Eastern Cape were purposefully selected. Lesson observations, semi-structured interviews, and document analysis (lesson plans) with three English FAL educators were used to collect data. Shulman’s (1986) Pedagogical Content Knowledge was used as the theoretical framework and analytical tool for the study. Data revealed ineffective teaching strategies and on-the-surface subject matter knowledge that hampers the critical teaching of short stories. It also showed that educators have limited knowledge of learners’ learning difficulties and how to address them. The study recommends less teacher talk that was observed in many classes, to be replaced by extended discussions on the part of the learners. Educators should establish a variety of contemporary teaching strategies which put critical thinking and active learning before examination preparation. Lastly, educators, in their teaching of short stories, should consult various resources and attend content workshops to keep subject knowledge up to date. , Thesis (MEd) -- Faculty of Humanities, Study of Englishes of Africa, 2023
- Full Text:
- Date Issued: 2023-10-13
Investigation into methods of recovering campylobacter spp. from river water samples
- Authors: Ngoni, Nandipha
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/424177 , vital:72130
- Description: Campylobacter species are slender, gram-negative, rod-shaped, spiral- or curved-shaped with single or pairs of flagella. They are the leading cause of diarrheal disease globally, consumption of and contact with water contaminated by faeces is a major risk factor for transmission of these organisms to humans. Rivers used for recreation and domestic and agricultural activities represent all the risk factors for Campylobacter spp. pollution and human exposure. Campylobacter spp. However, effective methods to recover Campylobacter spp. from river water samples are lacking, indicating the need for the development of more efficient methods of detection and isolation of these organisms from environmental water samples. Campylobacter detection in a water sample is critical to ascertain potential risks to humans. The aim of this study was to determine a suitable method for the detection of Campylobacter spp. from river water samples and the objectives were to (i) to evaluate the performance of different methods used for the recovery of Campylobacter spp. from environmental water samples based on Campylobacter colony count and PCR identification results, (ii) isolate and enumerate Campylobacter cells from river water samples, and (iii) identify Campylobacter spp. in river water samples. The Bloukrans River was chosen for this study because it is suspected to be contaminated by faecal inputs from nearby informal settlements without adequate sanitation, as well as untreated/insufficiently treated effluents from nearby wastewater treatment plants. First, the physicochemical quality of the river water and the presence of faecal contamination were assessed to confirm suitability for Campylobacter spp. survival and presence. Then different approaches to sample, concentrate and recover Campylobacter spp. from river water samples were assessed. The different methods assessed were (i) direct enrichment of water samples without prior concentration, (ii) prior concentration of water samples by centrifugation followed by membrane filtration of supernatant, and after that, pooling the residue and pellet together for enrichment, (iii) sampling by the Moore Swab technique. For all three methods, enrichment in Bolton broth supplemented with Bolton antibiotics was conducted. This was followed by plating on modified cefoperazone charcoal deoxycholate agar (mCCDA) and incubation under a microaerophilic atmosphere at 42°C for 48 h. Colony morphology, Gram staining and polymerase chain reaction (PCR) were used to identify and characterize the microorganisms. The growth of blue colonies on the mFc agar surface confirmed presence and faecal pollution of the Bloukrans River. The physicochemical properties, based on the range of pH measured at different sites of the river (between acidic 3.45 to 6.42 and alkaline 7.2 to 8.74) indicate that Campylobacter spp. can thrive in the river. Based on the results from enumeration and sequencing of colonies recovered by each method, it was discovered that the most suitable method to recover Campylobacter spp. from river water samples is by prior centrifugation (14,000 × g for 30 minutes) followed by membrane filtration of the supernatant, and subsequent pooling of the residue and pellet. The pooled residue and pellet might have increased Campylobacter spp. concentrations aiding more growth during the enrichment of Campylobacter spp. from the river water samples. Results from enumerating Campylobacter spp. cells from river water samples indicate that Campylobacter spp. are present in Bloukrans River. The sequence obtained from the PCR product indicates that the species found were Campylobacter jejuni (96% homology as evaluated by BLAST). This study provided a procedure effective for obtaining a satisfactory quantitative recovery of Campylobacter spp. from environmental waters, a critical need for quantitative microbial risk assessment studies. , Thesis (MSc) -- Faculty of Science, Institute for Water Research, 2023
- Full Text:
- Date Issued: 2023-10-13
- Authors: Ngoni, Nandipha
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/424177 , vital:72130
- Description: Campylobacter species are slender, gram-negative, rod-shaped, spiral- or curved-shaped with single or pairs of flagella. They are the leading cause of diarrheal disease globally, consumption of and contact with water contaminated by faeces is a major risk factor for transmission of these organisms to humans. Rivers used for recreation and domestic and agricultural activities represent all the risk factors for Campylobacter spp. pollution and human exposure. Campylobacter spp. However, effective methods to recover Campylobacter spp. from river water samples are lacking, indicating the need for the development of more efficient methods of detection and isolation of these organisms from environmental water samples. Campylobacter detection in a water sample is critical to ascertain potential risks to humans. The aim of this study was to determine a suitable method for the detection of Campylobacter spp. from river water samples and the objectives were to (i) to evaluate the performance of different methods used for the recovery of Campylobacter spp. from environmental water samples based on Campylobacter colony count and PCR identification results, (ii) isolate and enumerate Campylobacter cells from river water samples, and (iii) identify Campylobacter spp. in river water samples. The Bloukrans River was chosen for this study because it is suspected to be contaminated by faecal inputs from nearby informal settlements without adequate sanitation, as well as untreated/insufficiently treated effluents from nearby wastewater treatment plants. First, the physicochemical quality of the river water and the presence of faecal contamination were assessed to confirm suitability for Campylobacter spp. survival and presence. Then different approaches to sample, concentrate and recover Campylobacter spp. from river water samples were assessed. The different methods assessed were (i) direct enrichment of water samples without prior concentration, (ii) prior concentration of water samples by centrifugation followed by membrane filtration of supernatant, and after that, pooling the residue and pellet together for enrichment, (iii) sampling by the Moore Swab technique. For all three methods, enrichment in Bolton broth supplemented with Bolton antibiotics was conducted. This was followed by plating on modified cefoperazone charcoal deoxycholate agar (mCCDA) and incubation under a microaerophilic atmosphere at 42°C for 48 h. Colony morphology, Gram staining and polymerase chain reaction (PCR) were used to identify and characterize the microorganisms. The growth of blue colonies on the mFc agar surface confirmed presence and faecal pollution of the Bloukrans River. The physicochemical properties, based on the range of pH measured at different sites of the river (between acidic 3.45 to 6.42 and alkaline 7.2 to 8.74) indicate that Campylobacter spp. can thrive in the river. Based on the results from enumeration and sequencing of colonies recovered by each method, it was discovered that the most suitable method to recover Campylobacter spp. from river water samples is by prior centrifugation (14,000 × g for 30 minutes) followed by membrane filtration of the supernatant, and subsequent pooling of the residue and pellet. The pooled residue and pellet might have increased Campylobacter spp. concentrations aiding more growth during the enrichment of Campylobacter spp. from the river water samples. Results from enumerating Campylobacter spp. cells from river water samples indicate that Campylobacter spp. are present in Bloukrans River. The sequence obtained from the PCR product indicates that the species found were Campylobacter jejuni (96% homology as evaluated by BLAST). This study provided a procedure effective for obtaining a satisfactory quantitative recovery of Campylobacter spp. from environmental waters, a critical need for quantitative microbial risk assessment studies. , Thesis (MSc) -- Faculty of Science, Institute for Water Research, 2023
- Full Text:
- Date Issued: 2023-10-13
Investigation of brewery waste grains and microbial fuel cells as value-additive technologies improving solvent production yields in Clostridium acetobutylicum (ATCC 824) fermentation
- Authors: Du Toit, Ryan Guillaume
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/424643 , vital:72171
- Description: The production of the solvent compounds acetone, ethanol and butanol through fermentation of organic feedstocks using Clostridia species could be a promising route for biofuel production. However, the cost of raw materials, low yields and the complexity of anaerobic fermentation continue to hinder this means of generating these compounds. The research presented in this Thesis investigated low-cost interventions that could decrease the costs of production and to direct the synthesis of fuel compounds using microbial fuel cells. Low-cost anaerobic chambers were designed and constructed for the propagation and manipulation of Clostridium acetobutylicum, selected as a low-risk microbial catalyst. Fermentation was monitored using in situ pH measurements and a combination of turbidity measurements, nutrient assays (especially total carbohydrates) and HPLC-RI detection as a means of monitoring the consumption of nutrients (glucose), production of precursor compounds (butyric acid) and the formation of solvent molecules (acetone/ethanol and butanol) during fermentation by this organism. Brewer’s spent grains were tested as a sustainable and low-cost feedstock for solvent production, comparing the effects of sterilising before fermentation, or allowing resident microflora to remain during Clostridium-catalysed solvent production. Sterilised spent grains significantly improved the production of solvent molecules (e.g. 12.97 ± 0.38 g/L of butanol yielded, compared to 0.40 ± 0.33 g/L for defined media sampled during the solventogenic phase); compared to these, the use of non-sterilised brewer’s grain decreased both the reproducibility and yields of fermentation (8.66 ± 1.6 g/L of butanol). Microbial fuel cells were studied as a possible means of altering electron transfer to/from electrode-attached Clostridia to control the metabolic shift in bacteria from acidogenesis to solventogenesis. The base line MFC (11.00 ± 4.69 g/L) fermentation experiment did produce higher acetone/ethanol than the baseline batch experiment MB (5.47 ± 4.48 g/L), indicating an improvement to solvent production in C. acetobutylicum (ATCC 824) in a MFC fermentation. In this study, MFC-1 demonstrated remarkable superiority over MB in terms of butyric acid production, yielding significantly higher concentrations while also improving acetone and ethanol production. However, the enhanced butyric acid production did not correspond to significantly increased butanol yields when compared to batch fermentation of chemically defined media. These findings highlight the potential of MFC-1 as an efficient approach for enhancing the fermentative production of valuable compounds, with a particular focus on butyric acid and acetone/ethanol. , Thesis (MSc) -- Faculty of Science, Biotechnology Innovation Centre, 2023
- Full Text:
- Date Issued: 2023-10-13
- Authors: Du Toit, Ryan Guillaume
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/424643 , vital:72171
- Description: The production of the solvent compounds acetone, ethanol and butanol through fermentation of organic feedstocks using Clostridia species could be a promising route for biofuel production. However, the cost of raw materials, low yields and the complexity of anaerobic fermentation continue to hinder this means of generating these compounds. The research presented in this Thesis investigated low-cost interventions that could decrease the costs of production and to direct the synthesis of fuel compounds using microbial fuel cells. Low-cost anaerobic chambers were designed and constructed for the propagation and manipulation of Clostridium acetobutylicum, selected as a low-risk microbial catalyst. Fermentation was monitored using in situ pH measurements and a combination of turbidity measurements, nutrient assays (especially total carbohydrates) and HPLC-RI detection as a means of monitoring the consumption of nutrients (glucose), production of precursor compounds (butyric acid) and the formation of solvent molecules (acetone/ethanol and butanol) during fermentation by this organism. Brewer’s spent grains were tested as a sustainable and low-cost feedstock for solvent production, comparing the effects of sterilising before fermentation, or allowing resident microflora to remain during Clostridium-catalysed solvent production. Sterilised spent grains significantly improved the production of solvent molecules (e.g. 12.97 ± 0.38 g/L of butanol yielded, compared to 0.40 ± 0.33 g/L for defined media sampled during the solventogenic phase); compared to these, the use of non-sterilised brewer’s grain decreased both the reproducibility and yields of fermentation (8.66 ± 1.6 g/L of butanol). Microbial fuel cells were studied as a possible means of altering electron transfer to/from electrode-attached Clostridia to control the metabolic shift in bacteria from acidogenesis to solventogenesis. The base line MFC (11.00 ± 4.69 g/L) fermentation experiment did produce higher acetone/ethanol than the baseline batch experiment MB (5.47 ± 4.48 g/L), indicating an improvement to solvent production in C. acetobutylicum (ATCC 824) in a MFC fermentation. In this study, MFC-1 demonstrated remarkable superiority over MB in terms of butyric acid production, yielding significantly higher concentrations while also improving acetone and ethanol production. However, the enhanced butyric acid production did not correspond to significantly increased butanol yields when compared to batch fermentation of chemically defined media. These findings highlight the potential of MFC-1 as an efficient approach for enhancing the fermentative production of valuable compounds, with a particular focus on butyric acid and acetone/ethanol. , Thesis (MSc) -- Faculty of Science, Biotechnology Innovation Centre, 2023
- Full Text:
- Date Issued: 2023-10-13
InVisible freedom fighter: a critical analysis of portrayals of women in archival photographs, independence monuments and contemporary art in Zambia (Northern Rhodesia) and Zimbabwe (Southern Rhodesia)
- Authors: Kalichini, Gladys Melina
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/432433 , vital:72870 , DOI 10.21504/10962/432433
- Description: This doctoral dissertation in art history develops a notion of invisibility by critically analysing processes in which narratives about women are either concealed or uncovered in visual portrayals relating to the independence of Zambia (former Northern Rhodesia) and Zimbabwe (previously Southern Rhodesia). This study concentrates on three main visual categories that include archival photographs, national monuments, and visual art. It critically engages with concepts of memory and history through a framework of gender. The concept of invisibility developed in this thesis articulates a dynamic process in which independence narratives evolve over time, sometimes revealing memories associated with women and at other times rendering women invisible. National liberation in many African states is dominantly accredited to the political parties that were in power at the time of independence. In Zambia, the United National Independence Party (UNIP) is acknowledged for spearheading efforts to overthrow the colonial administration, while in Zimbabwe it is the Zimbabwe African National Union – Patriotic Front (ZANU – PF). Both political parties were headed by men, and the majority of their memberships was also comprised of men; as such, the dominant narratives largely illuminate the stories of men associated with these political parties. The overarching argument of this doctoral dissertation is that there is a gender bias inherent in dominant independence struggles narratives that are communicated through cultural heritage sites such as monuments and archives. In this study, art and art making inform theory as the methodological approach takes the direction in which selected artworks and visual materials are employed as a starting point of considering concepts that relate to the visibilities of stories about women. This approach cogitates the function of art, visual culture, and art history in the production of knowledges that foster in-depth understandings of concepts that explain social phenomena such as historical erasure. This doctoral dissertation in art history is divided into two parts, A and B, that conceptually complement each other. In section A which comprises of chapters one and two, the study develops an alternative visual archive that surveys the involvements of six specific women in the attainment of national independence in their respective countries, and critically analyses the Freedom Statue in Zambia and the National Heroes Acre in Zimbabwe as monuments dedicated to commemorating the independence struggle in the two countries. In Chapters three to five which form the second section of this dissertation, the emphasis of the discussion is on how selected visual artworks of three selected artists disrupt, counter or engage with dominant historical accounts that either exclude or marginalise narratives about women. The three artists include myself, Gladys Kalichini, and Zimbabwean born artists Kudzanai Chiurai and Gresham Tapiwa Nyaude. This thesis offers a culturally rich conversation about visual representations of social, political and cultural roles women performed in the colonial times in Northern and Southern Rhodesia and gives insight into the evolution of the luminosity of contemporary performances of women’s social collectives in Zambia and Zimbabwe. , Thesis (PhD) -- Faculty of Humanities, Fine Art, 2023
- Full Text:
- Date Issued: 2023-10-13
- Authors: Kalichini, Gladys Melina
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/432433 , vital:72870 , DOI 10.21504/10962/432433
- Description: This doctoral dissertation in art history develops a notion of invisibility by critically analysing processes in which narratives about women are either concealed or uncovered in visual portrayals relating to the independence of Zambia (former Northern Rhodesia) and Zimbabwe (previously Southern Rhodesia). This study concentrates on three main visual categories that include archival photographs, national monuments, and visual art. It critically engages with concepts of memory and history through a framework of gender. The concept of invisibility developed in this thesis articulates a dynamic process in which independence narratives evolve over time, sometimes revealing memories associated with women and at other times rendering women invisible. National liberation in many African states is dominantly accredited to the political parties that were in power at the time of independence. In Zambia, the United National Independence Party (UNIP) is acknowledged for spearheading efforts to overthrow the colonial administration, while in Zimbabwe it is the Zimbabwe African National Union – Patriotic Front (ZANU – PF). Both political parties were headed by men, and the majority of their memberships was also comprised of men; as such, the dominant narratives largely illuminate the stories of men associated with these political parties. The overarching argument of this doctoral dissertation is that there is a gender bias inherent in dominant independence struggles narratives that are communicated through cultural heritage sites such as monuments and archives. In this study, art and art making inform theory as the methodological approach takes the direction in which selected artworks and visual materials are employed as a starting point of considering concepts that relate to the visibilities of stories about women. This approach cogitates the function of art, visual culture, and art history in the production of knowledges that foster in-depth understandings of concepts that explain social phenomena such as historical erasure. This doctoral dissertation in art history is divided into two parts, A and B, that conceptually complement each other. In section A which comprises of chapters one and two, the study develops an alternative visual archive that surveys the involvements of six specific women in the attainment of national independence in their respective countries, and critically analyses the Freedom Statue in Zambia and the National Heroes Acre in Zimbabwe as monuments dedicated to commemorating the independence struggle in the two countries. In Chapters three to five which form the second section of this dissertation, the emphasis of the discussion is on how selected visual artworks of three selected artists disrupt, counter or engage with dominant historical accounts that either exclude or marginalise narratives about women. The three artists include myself, Gladys Kalichini, and Zimbabwean born artists Kudzanai Chiurai and Gresham Tapiwa Nyaude. This thesis offers a culturally rich conversation about visual representations of social, political and cultural roles women performed in the colonial times in Northern and Southern Rhodesia and gives insight into the evolution of the luminosity of contemporary performances of women’s social collectives in Zambia and Zimbabwe. , Thesis (PhD) -- Faculty of Humanities, Fine Art, 2023
- Full Text:
- Date Issued: 2023-10-13
Jazz pedagogical strategies: an A/R/Tographic investigation into the implementation of the South African jazz CAPS syllabus
- Authors: Rungan, Natalie
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/432488 , vital:72875 , DOI 10.21504/10962/432488
- Description: The Jazz stream of the Curriculum and Assessment Policy Statement Further Education and Training music curriculum was introduced in South Africa in 2012 (DBE, 2011a). Despite the intentions of the government to promote diversity, social transformation, and inclusion, subject music is still weighted towards Western Art Music, perpetuating past biases of a Eurocentric model for music education. This study, which uses a mixed-method approach, seeks to create strategies to advance Jazz education in South Africa through an a/r/tographic analysis of the author’s Jazz pedagogical methods at Durban High School in KwaZulu Natal, South Africa. Key approaches include curriculum theory, currere, a/r/tography, among others. After reviewing Jazz pedagogy in the U.S.A. and South Africa as a starting point, using Pinar’s (1994) method of currere, the author demonstrates how past personal music educational processes have led to present Jazz pedagogical methods. Six original compositions were written that outline the influences that infused these methods and added to the creative output related to this research. Interviews were conducted with key stakeholders in the Jazz community to gain perspective about the current state of Jazz education, and with students to provide insight into their reactions to the Jazz stream of CAPS. This presents new information about the curriculum from a learners’ perspective. Ethical clearance for research with children was sought and received (Appendix A). Findings show that the ability of learners to recognise the value in, and identity relating to, their African culture through Jazz points to a pivotal departure from previous Eurocentric music education models. However, despite South Africa being positioned as one of the only countries offering Jazz as a subject choice to high school learners, the selection of Jazz in the subject of Music remains underutilised. This study concludes that for Jazz education to advance in South African high schools, there needs to be intentional engagement with Jazz professionals at the high school level. , Thesis (PhD) -- Faculty of Humanities, Music and Musicology, 2023
- Full Text:
- Date Issued: 2023-10-13
- Authors: Rungan, Natalie
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/432488 , vital:72875 , DOI 10.21504/10962/432488
- Description: The Jazz stream of the Curriculum and Assessment Policy Statement Further Education and Training music curriculum was introduced in South Africa in 2012 (DBE, 2011a). Despite the intentions of the government to promote diversity, social transformation, and inclusion, subject music is still weighted towards Western Art Music, perpetuating past biases of a Eurocentric model for music education. This study, which uses a mixed-method approach, seeks to create strategies to advance Jazz education in South Africa through an a/r/tographic analysis of the author’s Jazz pedagogical methods at Durban High School in KwaZulu Natal, South Africa. Key approaches include curriculum theory, currere, a/r/tography, among others. After reviewing Jazz pedagogy in the U.S.A. and South Africa as a starting point, using Pinar’s (1994) method of currere, the author demonstrates how past personal music educational processes have led to present Jazz pedagogical methods. Six original compositions were written that outline the influences that infused these methods and added to the creative output related to this research. Interviews were conducted with key stakeholders in the Jazz community to gain perspective about the current state of Jazz education, and with students to provide insight into their reactions to the Jazz stream of CAPS. This presents new information about the curriculum from a learners’ perspective. Ethical clearance for research with children was sought and received (Appendix A). Findings show that the ability of learners to recognise the value in, and identity relating to, their African culture through Jazz points to a pivotal departure from previous Eurocentric music education models. However, despite South Africa being positioned as one of the only countries offering Jazz as a subject choice to high school learners, the selection of Jazz in the subject of Music remains underutilised. This study concludes that for Jazz education to advance in South African high schools, there needs to be intentional engagement with Jazz professionals at the high school level. , Thesis (PhD) -- Faculty of Humanities, Music and Musicology, 2023
- Full Text:
- Date Issued: 2023-10-13
Just stories?: Epistemic (in)justice and everyday resistance in the digital stories of family literacy practices by Grade 1-5 workers at a South African University
- Authors: Viedge, Jane Margaret
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/432308 , vital:72860
- Description: This thesis sets out to establish in what ways digital storytelling (DST) revealed instances of epistemic (in)justices inherent in the family literacy intervention experiences of four Black, working-class parents employed at a South African University1. Additionally, it explores how DST might be used by the participants (self-labelled the Storytelling Family Literacy - SFL Advocates) to correct any harm done. The context for the research is based on the deep-rooted harms of Bantu education in which Black South Africans were denied equal access to resources such as literacy practices. I investigated these aims by using the theories of DST, Communication for Social Change (C4SC) and epistemic (in)justice. As a participatory media practice supporting communications in marginalised communities, DST’s broad purpose is to assist these communities in telling stories of their lived experiences in ways that change dominant and, therefore, unchallenged views about them (Servaes & Malikhao 2014). In facilitating DST workshops with the SFL Advocates, I searched for evidence of epistemic (in)justice to enhance our (all stakeholders) understanding of their literacy experiences in ways that addressed their credibility status as ‘knowers’ (Fricker, 2007). Viewed through this lens, utilising DST provided a mechanism for understanding the impact of social interventions in a university setting that aimed to correct past injustices. The process involved online and face-to-face workshops with the participants during the Covid-19 Lockdown. The online format hampered data collection processes, and I resorted to face-to-face interactions for the workshops and interviews. These interactions were distinctly different from those I had experienced with participants in previous workshops, and they became a key focus in my analysis. Using narrative inquiry with critical realist and interpretive analysis techniques to interrogate the data, I discovered DST has both weaknesses and strengths as a C4SC communication tool. My findings revealed participants who, in the moment of leading their children and communities through the family literacy intervention, could claim to have redressed epistemic harms from the legacy of Apartheid education. However, by reflecting on this moment, the participants brought to light an epistemic harm that had been previously left unspoken: their sense of restored credibility as knowledge-bearers during the intervention was deeply shaken when the programme ended because they felt abandoned by the university. Therefore, the epistemic (in)justice lens unveiled hidden injustices that curtailed the participants’ ability to participate fully in the DST workshops. They described suffering the effects of pernicious and arbitrary acts of epistemic injustice at the hands of the University. Their participation in my workshops was erratic, and their stories were incomplete. As a result, I experienced difficulty analysing what I perceived as a lack of data. Moreover, despite my independent researcher status, my positionality as participant-observer was problematic as the participants may have perceived me as representative of the University and its institutional power. I responded to the problematic data by including a new theoretical framework in my analysis: the theory of everyday resistance (Vinthagen & Johansson, 2013). Combined with epistemic (in)justice theory and Vivienne’s ideas of using DST for everyday activism, I theorised how the participants negotiated their expressions of identity in epistemically unjust spaces. To put institutional epistemic injustice in perspective in the context of the University, I drew on Fricker’s three models of epistemic justice that enable fair and free conditions for marginalised workers to communicate for social change. , Thesis (MA) -- Faculty of Humanities, Journalism and Media Studies, 2023
- Full Text:
- Date Issued: 2023-10-13
- Authors: Viedge, Jane Margaret
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/432308 , vital:72860
- Description: This thesis sets out to establish in what ways digital storytelling (DST) revealed instances of epistemic (in)justices inherent in the family literacy intervention experiences of four Black, working-class parents employed at a South African University1. Additionally, it explores how DST might be used by the participants (self-labelled the Storytelling Family Literacy - SFL Advocates) to correct any harm done. The context for the research is based on the deep-rooted harms of Bantu education in which Black South Africans were denied equal access to resources such as literacy practices. I investigated these aims by using the theories of DST, Communication for Social Change (C4SC) and epistemic (in)justice. As a participatory media practice supporting communications in marginalised communities, DST’s broad purpose is to assist these communities in telling stories of their lived experiences in ways that change dominant and, therefore, unchallenged views about them (Servaes & Malikhao 2014). In facilitating DST workshops with the SFL Advocates, I searched for evidence of epistemic (in)justice to enhance our (all stakeholders) understanding of their literacy experiences in ways that addressed their credibility status as ‘knowers’ (Fricker, 2007). Viewed through this lens, utilising DST provided a mechanism for understanding the impact of social interventions in a university setting that aimed to correct past injustices. The process involved online and face-to-face workshops with the participants during the Covid-19 Lockdown. The online format hampered data collection processes, and I resorted to face-to-face interactions for the workshops and interviews. These interactions were distinctly different from those I had experienced with participants in previous workshops, and they became a key focus in my analysis. Using narrative inquiry with critical realist and interpretive analysis techniques to interrogate the data, I discovered DST has both weaknesses and strengths as a C4SC communication tool. My findings revealed participants who, in the moment of leading their children and communities through the family literacy intervention, could claim to have redressed epistemic harms from the legacy of Apartheid education. However, by reflecting on this moment, the participants brought to light an epistemic harm that had been previously left unspoken: their sense of restored credibility as knowledge-bearers during the intervention was deeply shaken when the programme ended because they felt abandoned by the university. Therefore, the epistemic (in)justice lens unveiled hidden injustices that curtailed the participants’ ability to participate fully in the DST workshops. They described suffering the effects of pernicious and arbitrary acts of epistemic injustice at the hands of the University. Their participation in my workshops was erratic, and their stories were incomplete. As a result, I experienced difficulty analysing what I perceived as a lack of data. Moreover, despite my independent researcher status, my positionality as participant-observer was problematic as the participants may have perceived me as representative of the University and its institutional power. I responded to the problematic data by including a new theoretical framework in my analysis: the theory of everyday resistance (Vinthagen & Johansson, 2013). Combined with epistemic (in)justice theory and Vivienne’s ideas of using DST for everyday activism, I theorised how the participants negotiated their expressions of identity in epistemically unjust spaces. To put institutional epistemic injustice in perspective in the context of the University, I drew on Fricker’s three models of epistemic justice that enable fair and free conditions for marginalised workers to communicate for social change. , Thesis (MA) -- Faculty of Humanities, Journalism and Media Studies, 2023
- Full Text:
- Date Issued: 2023-10-13
Kant and the experience of skepticism: rranscendental arguments, skepticism, and a version of the problem of the justification of foundational assumptions
- Authors: Grecia, Hadley
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/425078 , vital:72207
- Description: Immanuel Kant is a key thinker in the History of Western Philosophy whose ideas continue to fascinate contemporary English-speaking academic philosophers. One such idea is Kant's conception of transcendental arguments. Although these arguments do not originate in Kant's philosophy, Kant's conception of them occupies a special place in the English-speaking literature on the topic. A growing trend in this literature is to claim that Kant's transcendental arguments are unique because they aim to achieve an anti-skeptical end through deductive means. I call the basic assumption behind readings of transcendental arguments like these the deductive/anti-skeptical assumption. This assumption claims that deduction is the best means to achieve an anti-skeptical end. In this thesis, I argue that accepting this assumption is one way to misinterpret transcendental arguments because—even though it seems compelling at first blush—it must be false. The resultant twist is that transcendental arguments cannot be deductive, which, in turn, means that understanding that Kant's transcendental arguments are not deductive in nature is itself no small detail for understanding the version of Kant's 'not-deductive' solution to skepticism developed here. , Thesis (MA) -- Faculty of Humanities, Philosophy, 2023
- Full Text:
- Date Issued: 2023-10-13
- Authors: Grecia, Hadley
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/425078 , vital:72207
- Description: Immanuel Kant is a key thinker in the History of Western Philosophy whose ideas continue to fascinate contemporary English-speaking academic philosophers. One such idea is Kant's conception of transcendental arguments. Although these arguments do not originate in Kant's philosophy, Kant's conception of them occupies a special place in the English-speaking literature on the topic. A growing trend in this literature is to claim that Kant's transcendental arguments are unique because they aim to achieve an anti-skeptical end through deductive means. I call the basic assumption behind readings of transcendental arguments like these the deductive/anti-skeptical assumption. This assumption claims that deduction is the best means to achieve an anti-skeptical end. In this thesis, I argue that accepting this assumption is one way to misinterpret transcendental arguments because—even though it seems compelling at first blush—it must be false. The resultant twist is that transcendental arguments cannot be deductive, which, in turn, means that understanding that Kant's transcendental arguments are not deductive in nature is itself no small detail for understanding the version of Kant's 'not-deductive' solution to skepticism developed here. , Thesis (MA) -- Faculty of Humanities, Philosophy, 2023
- Full Text:
- Date Issued: 2023-10-13
Kenyan comedy: transmogrifying stereotypes and fashioning a ‘Kenyanness’ of aesthetic escapism
- Authors: Lumasia, Patrick Chesi
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/432477 , vital:72874 , DOI 10.21504/10962/432477
- Description: Despite the proliferation of stereotypes in Kenyan comedy, little research exists to show the two intersect to shape a peculiar notion of ‘Kenyanness’. Indeed, Kenyan comedy, besides reproducing and playing on the quotidian and the historical, is heavily invested with ethnic and gender stereotypes that reflect the Kenyan milieu as it intersects with the global. This comedy is oftentimes seen by some critics as detrimental to Kenyan society because it supposedly shapes and reinforces ethnic and/or gender relations in the country. However, this study contends that Kenyan comedy is open to multiple interpretations and meaning contestations that are not necessarily clear to the comedians and audiences due to the asymmetry that abounds between the production and consumption ends of the comedy’s spectrum. The comedy is therefore ambivalent. This research seeks to demonstrate that Kenyan comedy—as a form of entertainment and critique of society—does not seek to fix and reify Kenyan identities. Instead, the comedy frees these identities from the presumed vice-hold, constricting world of stereotypes by disrupting the linearity of the stereotypes, thereby unsettling the hierarchical structure of hegemonic ideology embedded in them through postmodern humour: a form of humour amenable to postmodern sensibilities. To this end, the comedy offers Kenyans momentary escape into a comedic utopia, through which, they address pertinent issues affecting their nationhood, even as they endeavor to fashion a ‘Kenyanness’ of aesthetic escapism that is celebratory of the country’s rich socio-cultural diversity. The study employs Jörg Schweinitz’s (2011) stereotype theory and the postpositivist realist theory of identity in its study of stand-up, scripted episodic and topical comedy as transposed on to YouTube. Specifically, the thesis considers: the Churchill Show (2012 – 2022) that aired on NTV; 2012–2022; The Real Househelps of Kawangware (2014–2021) on KTN/NTV; Auntie Boss (2016–2021) on NTV; The Wicked Edition and The Trending Trend Talkers (2014–). , Thesis (PhD) -- Faculty of Humanities, Literary Studies in English, 2023
- Full Text:
- Date Issued: 2023-10-13
- Authors: Lumasia, Patrick Chesi
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/432477 , vital:72874 , DOI 10.21504/10962/432477
- Description: Despite the proliferation of stereotypes in Kenyan comedy, little research exists to show the two intersect to shape a peculiar notion of ‘Kenyanness’. Indeed, Kenyan comedy, besides reproducing and playing on the quotidian and the historical, is heavily invested with ethnic and gender stereotypes that reflect the Kenyan milieu as it intersects with the global. This comedy is oftentimes seen by some critics as detrimental to Kenyan society because it supposedly shapes and reinforces ethnic and/or gender relations in the country. However, this study contends that Kenyan comedy is open to multiple interpretations and meaning contestations that are not necessarily clear to the comedians and audiences due to the asymmetry that abounds between the production and consumption ends of the comedy’s spectrum. The comedy is therefore ambivalent. This research seeks to demonstrate that Kenyan comedy—as a form of entertainment and critique of society—does not seek to fix and reify Kenyan identities. Instead, the comedy frees these identities from the presumed vice-hold, constricting world of stereotypes by disrupting the linearity of the stereotypes, thereby unsettling the hierarchical structure of hegemonic ideology embedded in them through postmodern humour: a form of humour amenable to postmodern sensibilities. To this end, the comedy offers Kenyans momentary escape into a comedic utopia, through which, they address pertinent issues affecting their nationhood, even as they endeavor to fashion a ‘Kenyanness’ of aesthetic escapism that is celebratory of the country’s rich socio-cultural diversity. The study employs Jörg Schweinitz’s (2011) stereotype theory and the postpositivist realist theory of identity in its study of stand-up, scripted episodic and topical comedy as transposed on to YouTube. Specifically, the thesis considers: the Churchill Show (2012 – 2022) that aired on NTV; 2012–2022; The Real Househelps of Kawangware (2014–2021) on KTN/NTV; Auntie Boss (2016–2021) on NTV; The Wicked Edition and The Trending Trend Talkers (2014–). , Thesis (PhD) -- Faculty of Humanities, Literary Studies in English, 2023
- Full Text:
- Date Issued: 2023-10-13
Labour law and non-standard employment: case studies of selected workplaces in Makhanda
- Authors: Mini, Sifanelwa
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/425171 , vital:72216
- Description: While precarious and poorly-regulated forms of employment are on the increase worldwide, the patterns vary significantly over time and space. This study sought to understand employees’ perceptions of non-standard employment (NSE), the reasons why employers resort to NSE and why they use particular types of NSE. In addition, the research explored how labour law protections for NSE are applied in particular workplaces. Drawing on the regulation approach, which provides a conceptual framework to explain changes in production and employment relationships under specific spatio-temporal conditions, this study focused on three companies in Makhanda. Furthermore, this study employed a qualitative research method to explore the employees’ perceptions on non-standard work. This was done by using an interpretive approach to explore how workers experience NSE and how Eastern Cape citizens in Makhanda navigate the scourge of unemployment and exploitation in the labour market. Moreover, the research investigated how employees experience the efficacy of their employment rights in the workplace. The employment rights observed in the study include (among others) leave, contract of employment and collective bargaining; all of which are provisions in the Labour Relations Act. The findings in the study do validate the use of NSE as a ‘tool’ to curb high unemployment. However, the study finds that the positive result of job creation does not signify job security for non-standard workers, instead beneath the rhetoric of job creation and investment, lies hidden the exploitation of vulnerable groups of citizens and extension of precarious forms of employment. This is because businesses engage in economic competition locally and globally. Therefore, in order to successfully compete against other, employer’s cut on labour expenses by adopting a “race to the bottom,” the effect of which is paying employees below the minimum wage. Moreover, this study opposes the argument that NSE is a stepping stone to permanent employment. The conditions which prevail in NSE are distinctly different from those in the Standard Employment Relationship (SER), making it difficult for employees to transition to a new labour market. The latter is more aligned with the heterodox approach than it is to the orthodox approach to labour market. , Thesis (MA) -- Faculty of Humanities, Sociology, 2023
- Full Text:
- Date Issued: 2023-10-13
- Authors: Mini, Sifanelwa
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/425171 , vital:72216
- Description: While precarious and poorly-regulated forms of employment are on the increase worldwide, the patterns vary significantly over time and space. This study sought to understand employees’ perceptions of non-standard employment (NSE), the reasons why employers resort to NSE and why they use particular types of NSE. In addition, the research explored how labour law protections for NSE are applied in particular workplaces. Drawing on the regulation approach, which provides a conceptual framework to explain changes in production and employment relationships under specific spatio-temporal conditions, this study focused on three companies in Makhanda. Furthermore, this study employed a qualitative research method to explore the employees’ perceptions on non-standard work. This was done by using an interpretive approach to explore how workers experience NSE and how Eastern Cape citizens in Makhanda navigate the scourge of unemployment and exploitation in the labour market. Moreover, the research investigated how employees experience the efficacy of their employment rights in the workplace. The employment rights observed in the study include (among others) leave, contract of employment and collective bargaining; all of which are provisions in the Labour Relations Act. The findings in the study do validate the use of NSE as a ‘tool’ to curb high unemployment. However, the study finds that the positive result of job creation does not signify job security for non-standard workers, instead beneath the rhetoric of job creation and investment, lies hidden the exploitation of vulnerable groups of citizens and extension of precarious forms of employment. This is because businesses engage in economic competition locally and globally. Therefore, in order to successfully compete against other, employer’s cut on labour expenses by adopting a “race to the bottom,” the effect of which is paying employees below the minimum wage. Moreover, this study opposes the argument that NSE is a stepping stone to permanent employment. The conditions which prevail in NSE are distinctly different from those in the Standard Employment Relationship (SER), making it difficult for employees to transition to a new labour market. The latter is more aligned with the heterodox approach than it is to the orthodox approach to labour market. , Thesis (MA) -- Faculty of Humanities, Sociology, 2023
- Full Text:
- Date Issued: 2023-10-13
Leverage points for meaningful participatory governance: lessons from the Tsitsa River catchment, South Africa
- Authors: Fry, Anthony St Leger
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/431714 , vital:72798 , DOI 10.21504/10962/431714
- Description: South Africa underwent the renowned transition into a representative multiracial democracy in 1994. While there has been notable progress in many developmental aspects of South African society, many inherited dilemmas persist, and myriad novel ones are emerging. In land and water governance, the nascent institutions do not reflect the visions laid out in the pioneering and substantive legislation, policies, and guidelines generated in the post-1994 period. Unaddressed dilemmas include widespread failures in local water governance, persistent inequality of access to land and water, poor or non-existent service delivery in rural areas, underdeveloped institutions for integrated and inclusive water resource management, and pernicious divisions between institutions. Overcoming these challenges, which are embedded in complex social-ecological systems across South Africa, will require the collaborative effort of diverse actors from different levels and sectors of society. So how do we foster meaningful participation in ways that are not tyrannical, tokenistic, or manipulative? How do we build local institutions that make sense in the broader political system and in the lives of rural residents? How do we support institutions that meaningfully include diverse voices and enable tangible development outcomes? This thesis explores these questions as part of the Tsitsa Project, a transdisciplinary landscape management project working in the Tsitsa River Catchment (TsRC) in the rural parts of the Eastern Cape province of South Africa. Based on the valuable water resources, severe ecosystem degradation, overlapping traditional and democratic governance systems, and impoverished population, the TsRC is a worthwhile candidate for in-depth study paired with innovative efforts towards systemic development. The single place-based case study approach drew on systems thinking within an evolving transdisciplinary methodology. Qualitative data were collected through extended fieldwork and analysed through an adaptive and iterative approach. Governance mapping elucidated the multiple levels of governance, and a systemic analysis explored meaningful participation at the local level through causal diagramming and observation-based narratives. From the findings, a synthetic analysis identified high leverage points to enable participatory governance interventions to have more sustained impacts. Governance manifestations in the TsRC generally align with existing descriptions of the fractures and associated dilemmas across South Africa, with the added complexities of being a rural landscape in which democratic and traditional governance systems overlap and interact. Local participatory institutions need to endure the broader instability and dysfunction. Interventions must, therefore, be oriented towards trust building and shared understanding while using more practical interventions that provide tangible outcomes, enable in-practice capacity development, and support platforms for all actors to experience and practice meaningful participation together. This thesis aims to unearth the lessons that one small rural catchment might hold for the governance of complex, contested land and for water governance contexts more broadly. , Thesis (PhD) -- Faculty of Science, Institute for Water Research, 2023
- Full Text:
- Date Issued: 2023-10-13
- Authors: Fry, Anthony St Leger
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/431714 , vital:72798 , DOI 10.21504/10962/431714
- Description: South Africa underwent the renowned transition into a representative multiracial democracy in 1994. While there has been notable progress in many developmental aspects of South African society, many inherited dilemmas persist, and myriad novel ones are emerging. In land and water governance, the nascent institutions do not reflect the visions laid out in the pioneering and substantive legislation, policies, and guidelines generated in the post-1994 period. Unaddressed dilemmas include widespread failures in local water governance, persistent inequality of access to land and water, poor or non-existent service delivery in rural areas, underdeveloped institutions for integrated and inclusive water resource management, and pernicious divisions between institutions. Overcoming these challenges, which are embedded in complex social-ecological systems across South Africa, will require the collaborative effort of diverse actors from different levels and sectors of society. So how do we foster meaningful participation in ways that are not tyrannical, tokenistic, or manipulative? How do we build local institutions that make sense in the broader political system and in the lives of rural residents? How do we support institutions that meaningfully include diverse voices and enable tangible development outcomes? This thesis explores these questions as part of the Tsitsa Project, a transdisciplinary landscape management project working in the Tsitsa River Catchment (TsRC) in the rural parts of the Eastern Cape province of South Africa. Based on the valuable water resources, severe ecosystem degradation, overlapping traditional and democratic governance systems, and impoverished population, the TsRC is a worthwhile candidate for in-depth study paired with innovative efforts towards systemic development. The single place-based case study approach drew on systems thinking within an evolving transdisciplinary methodology. Qualitative data were collected through extended fieldwork and analysed through an adaptive and iterative approach. Governance mapping elucidated the multiple levels of governance, and a systemic analysis explored meaningful participation at the local level through causal diagramming and observation-based narratives. From the findings, a synthetic analysis identified high leverage points to enable participatory governance interventions to have more sustained impacts. Governance manifestations in the TsRC generally align with existing descriptions of the fractures and associated dilemmas across South Africa, with the added complexities of being a rural landscape in which democratic and traditional governance systems overlap and interact. Local participatory institutions need to endure the broader instability and dysfunction. Interventions must, therefore, be oriented towards trust building and shared understanding while using more practical interventions that provide tangible outcomes, enable in-practice capacity development, and support platforms for all actors to experience and practice meaningful participation together. This thesis aims to unearth the lessons that one small rural catchment might hold for the governance of complex, contested land and for water governance contexts more broadly. , Thesis (PhD) -- Faculty of Science, Institute for Water Research, 2023
- Full Text:
- Date Issued: 2023-10-13
Lifting the veil on the "Untouchable": a study of Muslims living with HIV in Durban, South Africa
- Authors: Shaik, Shabnam
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/432518 , vital:72877 , DOI 10.21504/10962/432519
- Description: HIV and AIDS has been prominently studied, by both biomedical and social scientists, since the 1980s. Despite the extensive research that has emerged globally, Muslims have largely been absent in much of the discourse on HIV and AIDS. While the disease is said to be under control in many parts of the world, hidden populations pose a risk to increase in prevalence. Muslims living with HIV are one such hidden population and are only recently gaining representation in studies on HIV and AIDS. Quantitative studies reflect a sharp increase in HIV prevalence in countries with significant Muslim populations, however, more in-depth qualitative studies are needed to garner an understanding of the nature of the disease among Muslims. Muslims have a long history in South Africa, beginning with indentured slavery in Natal (now KwaZulu-Natal) in 1860. There are no specific HIV and AIDS prevention campaigns directed at Muslims in Durban, KwaZulu-Natal, South Africa. Muslims in Durban do not regard HIV is a significant concern for them due to religious doctrine, which they believe if followed will keep them safe from infection. My findings suggest that there is cause for concern over the spread of HIV and AIDS amongst Muslims in Durban. The hidden nature of the disease in the Muslim community has created challenges to curbing the spread of the disease due to the concealment of its presence and the marginalisation of Muslims living with HIV and AIDS. This study conceptualises the HIV and AIDS lived experience amongst Muslims in Durban, South Africa, and uncovers the social and cultural context of the disease. The qualitative study used life histories with ten Muslims living with HIV and semi-structured and unstructured interviews with ten caregivers, five health care professionals and two religious leaders to gain a detailed understanding of the lived experiences of Muslims in Durban. Through the lenses of Purity and Danger, Stigma and Spoilt Identity, and Social Death, this study found that religion [Islam] and its strong moral code influences understandings and perceptions of HIV and AIDS which, in turn affects diagnosis, treatment, care, social identity, and the social well-being of Muslims living with HIV who face stigma, discrimination, shame, and ostracisation from their own community. This study highlights the gaps in the literature on Muslims living with HIV and by providing insight into the lives of participants, the study draws attention to the plight of Muslims living with HIV and AIDS (MLWHIV) and encourages more qualitative studies to be conducted so that a multifaceted understanding of Muslims living with HIV can be created. , Thesis (PhD) -- Faculty of Humanities, Anthropology, 2023
- Full Text:
- Date Issued: 2023-10-13
- Authors: Shaik, Shabnam
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/432518 , vital:72877 , DOI 10.21504/10962/432519
- Description: HIV and AIDS has been prominently studied, by both biomedical and social scientists, since the 1980s. Despite the extensive research that has emerged globally, Muslims have largely been absent in much of the discourse on HIV and AIDS. While the disease is said to be under control in many parts of the world, hidden populations pose a risk to increase in prevalence. Muslims living with HIV are one such hidden population and are only recently gaining representation in studies on HIV and AIDS. Quantitative studies reflect a sharp increase in HIV prevalence in countries with significant Muslim populations, however, more in-depth qualitative studies are needed to garner an understanding of the nature of the disease among Muslims. Muslims have a long history in South Africa, beginning with indentured slavery in Natal (now KwaZulu-Natal) in 1860. There are no specific HIV and AIDS prevention campaigns directed at Muslims in Durban, KwaZulu-Natal, South Africa. Muslims in Durban do not regard HIV is a significant concern for them due to religious doctrine, which they believe if followed will keep them safe from infection. My findings suggest that there is cause for concern over the spread of HIV and AIDS amongst Muslims in Durban. The hidden nature of the disease in the Muslim community has created challenges to curbing the spread of the disease due to the concealment of its presence and the marginalisation of Muslims living with HIV and AIDS. This study conceptualises the HIV and AIDS lived experience amongst Muslims in Durban, South Africa, and uncovers the social and cultural context of the disease. The qualitative study used life histories with ten Muslims living with HIV and semi-structured and unstructured interviews with ten caregivers, five health care professionals and two religious leaders to gain a detailed understanding of the lived experiences of Muslims in Durban. Through the lenses of Purity and Danger, Stigma and Spoilt Identity, and Social Death, this study found that religion [Islam] and its strong moral code influences understandings and perceptions of HIV and AIDS which, in turn affects diagnosis, treatment, care, social identity, and the social well-being of Muslims living with HIV who face stigma, discrimination, shame, and ostracisation from their own community. This study highlights the gaps in the literature on Muslims living with HIV and by providing insight into the lives of participants, the study draws attention to the plight of Muslims living with HIV and AIDS (MLWHIV) and encourages more qualitative studies to be conducted so that a multifaceted understanding of Muslims living with HIV can be created. , Thesis (PhD) -- Faculty of Humanities, Anthropology, 2023
- Full Text:
- Date Issued: 2023-10-13
Liposomal formulations of metallophthalocyanines-nanoparticle conjugates for hypoxic photodynamic therapy and photoelectrocatalysis
- Authors: Nwahara, Nnamdi Ugochinyere
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/432159 , vital:72847 , DOI 10.21504/10962/432159
- Description: This thesis investigates new strategies to enhance the efficacy of photodynamic therapy (PDT) under hypoxic conditions using in-vitro cancer cell models. Phthalocyanines are chosen as viable photosensitizer complexes owing to the favourable absorption properties. To this end, this thesis reports on the synthesis and photophysicochemical properties of various zinc and silicon phthalocyanines (Pcs). To afford better photophysicochemical properties, the reported Pcs were conjugated to different nanoparticles (NPs) through chemisorption as well as amide bond formation to yield Pc-NP conjugates. All the studied Pcs showed relatively high triplet and singlet oxygen quantum yields corresponding to their low fluorescence quantum yields. The various mechanisms for hypoxic response include (i) Type I PDT, (ii) PDT coupled with oxygen-independent therapy and (iii) in-situ oxygen generation using catalase-mimicking nanoparticles which serve to supplement in-vitro oxygen concentrations using MPcs or MPc-NPs conjugates. The mechanisms were assessed using electrochemical, computational techniques and catalase mimicking experiments. The as-synthesised Pcs or Pc-NPs were subjected to liposomal loading before PDT studies which led to enhanced biocompatibility and aqueous dispersity. The in-vitro dark cytotoxicity tests and photodynamic therapy activities of the fabricated Pc-liposomes and Pc-NPs-liposomes on either Henrietta Lacks (HeLa) or Michigan Cancer Foundation-7 (MCF-7) breast cancer cells are presented herein. This work further showed that folic acid (FA) functionalization of liposomes could be exploited for active drug delivery and herein led to an almost 3-fold increase in drug uptake vs non-FA functionalised liposomes in accordance with folate receptor (FR) expression levels between HeLa and MCF-7 cells. The in-vitro dark cytotoxicity and photodynamic therapy of selected Pc complexes and conjugates were accessed using MCF-7 and HeLa cell lines. The various mechanisms; (i) Type I PDT, (ii) PDT coupled with oxygen -independent therapy and (iii) in-situ oxygen generation using catalase-mimicking nanoparticles were shown to adequately compensate for the otherwise attenuation of PDT activity under hypoxia. , Thesis (PhD) -- Faculty of Science, Chemistry, 2023
- Full Text:
- Date Issued: 2023-10-13
- Authors: Nwahara, Nnamdi Ugochinyere
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/432159 , vital:72847 , DOI 10.21504/10962/432159
- Description: This thesis investigates new strategies to enhance the efficacy of photodynamic therapy (PDT) under hypoxic conditions using in-vitro cancer cell models. Phthalocyanines are chosen as viable photosensitizer complexes owing to the favourable absorption properties. To this end, this thesis reports on the synthesis and photophysicochemical properties of various zinc and silicon phthalocyanines (Pcs). To afford better photophysicochemical properties, the reported Pcs were conjugated to different nanoparticles (NPs) through chemisorption as well as amide bond formation to yield Pc-NP conjugates. All the studied Pcs showed relatively high triplet and singlet oxygen quantum yields corresponding to their low fluorescence quantum yields. The various mechanisms for hypoxic response include (i) Type I PDT, (ii) PDT coupled with oxygen-independent therapy and (iii) in-situ oxygen generation using catalase-mimicking nanoparticles which serve to supplement in-vitro oxygen concentrations using MPcs or MPc-NPs conjugates. The mechanisms were assessed using electrochemical, computational techniques and catalase mimicking experiments. The as-synthesised Pcs or Pc-NPs were subjected to liposomal loading before PDT studies which led to enhanced biocompatibility and aqueous dispersity. The in-vitro dark cytotoxicity tests and photodynamic therapy activities of the fabricated Pc-liposomes and Pc-NPs-liposomes on either Henrietta Lacks (HeLa) or Michigan Cancer Foundation-7 (MCF-7) breast cancer cells are presented herein. This work further showed that folic acid (FA) functionalization of liposomes could be exploited for active drug delivery and herein led to an almost 3-fold increase in drug uptake vs non-FA functionalised liposomes in accordance with folate receptor (FR) expression levels between HeLa and MCF-7 cells. The in-vitro dark cytotoxicity and photodynamic therapy of selected Pc complexes and conjugates were accessed using MCF-7 and HeLa cell lines. The various mechanisms; (i) Type I PDT, (ii) PDT coupled with oxygen -independent therapy and (iii) in-situ oxygen generation using catalase-mimicking nanoparticles were shown to adequately compensate for the otherwise attenuation of PDT activity under hypoxia. , Thesis (PhD) -- Faculty of Science, Chemistry, 2023
- Full Text:
- Date Issued: 2023-10-13
M3: Mining Mini-Halos with MeerKAT
- Authors: Trehaeven, Keegan Somerset
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/424754 , vital:72181
- Description: This work aims to showcase the MeerKAT telescope’s capabilities and related calibration and imaging software in studying the emission of radio mini-halos. These diffuse radio synchrotron sources surround a Brightest Cluster Galaxy (BCG) in relatively relaxed clusters out to a few 100 kpc in size. They are difficult to image because of their relatively low surface brightness and small angular size. Hence, they could not be studied in great detail by previous generations of radio telescopes and much about their nature, particularly the exact production mechanism, is not yet fully understood. Thus, for the first time, MeerKAT observed a sample of five galaxy clusters to investigate the central radio mini-halo in each. Studying these sources requires the deepest images generated from the data and the effective subtraction of any projected sources obscuring or contaminating the underlying diffuse emission. Therefore, I describe the data reduction used to create third-generation calibrated, primary beam corrected, point source subtracted Stokes I L-band continuum images of these clusters. For first- and second-generation calibration, I use the CARACal pipeline, which implements software optimised explicitly for MeerKAT data. For third-generation calibration, I use the faceted approach of killMS and DDFacet, and then I perform visibility-plane point source subtraction to disentangle the compact and diffuse emissions. I then measured the size, flux density, in-band spectral properties, and radio power of the central mini-halos. I present the first new mini-halo detection by MeerKAT (MACS J2140.2-2339, Trehaeven et al. accepted), the first spectral index maps of these mini-halos, which show very interesting distributions, and a ∼100 kpc II southern extension to the ACO 3444 mini-halo previously unseen in archival VLA data. Thereafter, I present a multi-wavelength case study for two complementary mini-halos from our sample and show via a radio-to-X-ray spatial correlation test that they might be caused by different particle (re)-acceleration mechanisms. Through these initial science results, I have shown that future observations of radio mini-halos with MeerKAT are an exciting prospect that can lead to a better understanding of the fundamental physics behind these sources. , Thesis (MSc) -- Faculty of Science, Physics and Electronics, 2023
- Full Text:
- Date Issued: 2023-10-13
- Authors: Trehaeven, Keegan Somerset
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/424754 , vital:72181
- Description: This work aims to showcase the MeerKAT telescope’s capabilities and related calibration and imaging software in studying the emission of radio mini-halos. These diffuse radio synchrotron sources surround a Brightest Cluster Galaxy (BCG) in relatively relaxed clusters out to a few 100 kpc in size. They are difficult to image because of their relatively low surface brightness and small angular size. Hence, they could not be studied in great detail by previous generations of radio telescopes and much about their nature, particularly the exact production mechanism, is not yet fully understood. Thus, for the first time, MeerKAT observed a sample of five galaxy clusters to investigate the central radio mini-halo in each. Studying these sources requires the deepest images generated from the data and the effective subtraction of any projected sources obscuring or contaminating the underlying diffuse emission. Therefore, I describe the data reduction used to create third-generation calibrated, primary beam corrected, point source subtracted Stokes I L-band continuum images of these clusters. For first- and second-generation calibration, I use the CARACal pipeline, which implements software optimised explicitly for MeerKAT data. For third-generation calibration, I use the faceted approach of killMS and DDFacet, and then I perform visibility-plane point source subtraction to disentangle the compact and diffuse emissions. I then measured the size, flux density, in-band spectral properties, and radio power of the central mini-halos. I present the first new mini-halo detection by MeerKAT (MACS J2140.2-2339, Trehaeven et al. accepted), the first spectral index maps of these mini-halos, which show very interesting distributions, and a ∼100 kpc II southern extension to the ACO 3444 mini-halo previously unseen in archival VLA data. Thereafter, I present a multi-wavelength case study for two complementary mini-halos from our sample and show via a radio-to-X-ray spatial correlation test that they might be caused by different particle (re)-acceleration mechanisms. Through these initial science results, I have shown that future observations of radio mini-halos with MeerKAT are an exciting prospect that can lead to a better understanding of the fundamental physics behind these sources. , Thesis (MSc) -- Faculty of Science, Physics and Electronics, 2023
- Full Text:
- Date Issued: 2023-10-13
Macroplastics in the environment: are they suitable habitats for macroinvertebrates in riverine systems?
- Authors: Ali, Andrew Abagai
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/424153 , vital:72128
- Description: Emerging pollutants, such as plastics are threat to freshwater ecosystems, and may negatively impact riverine systems. They can modify riverine habitats and affect aquatic organism distribution and composition. Knowledge of how macroplastics alter riverine habitat heterogeneity, and their effects on macroinvertebrate assemblage structure is sparse, especially in Africa. This study examines the effect of hydraulic biotopes on the colonisation, establishment and succession patterns of macroinvertebrates on macroplastic and natural substrates based on the taxonomic and trait-based approach. Four experimental sites from minimally impacted upper reaches of the Buffalo, Kat, Kowie, and Swartkops Rivers in the Eastern Cape of South Africa were selected for the deployment of plastic substrates. Plastics materials, including polyethylene terephthalate (PET) bottles and natural substrate composed of stone and vegetation, were used to formulate three substrate groups: Group 1: 100% natural substrates (NS), Group 2: 50% natural substrates and 50% plastic material (NP), and Group 3: 100% plastic materials (PD). These substrates were placed in litter bags of equal dimension (25 cm by 35 cm, with 2.5 cm mesh) and deployed randomly in three hydraulic biotopes (pools, riffles, runs) over a period of 180 days (October 2021 to April 2022). A total of 216 substrate bags, 54 bags per substrate were deployed per site in the four experimental sites. Twelve bags from each substrate group were retrieved at an interval of 30 days beginning on day 30 after deployment, and analysed for the establishment of macroinvertebrate communities. Based on composite hydraulic biotope data, Simpson index was significantly higher (P < 0.05) for macroinvertebrate assemblage structure on the 50% and 100% macroplastic substrate groups compared to natural substrates. With the exception of Tabanidae, Glossosomatidae, and Psephenidae, all macroinvertebrate taxa recorded showed non-significant positive correlations with all three substrate groups. However, Tabanidae, Glossosomatidae, and Psephenidae showed significant positive correlation with the 100% natural substrates, 50% plastic substrates and 100% plastic substrates, respectively. The parsimony analysis reveal that, within 30 days, all substrate groups underwent similar succession, with high abundance of pioneer taxa which increased on days 60 and 90, and then decreased from days 120 to 180. For the the pool biotope, Shannon and Simpson indices were significantly higher (P < 0.05) for the macroinvertabrates collected over the natural substates compared with those collected on the macroplastic substrate groups. However, in the riffle and run biotopes, all diversity indices were similar for all substrate groups and no statistically significant difference was observed. Statistically significant higher values for taxonomic richness, diversity, and evenness were found on day 30 to 90 for the riffle biotopes, and day 30 to 60 for the run biotopes. The run biotope presented temporal statistical significant variability in taxonomic composition with different macroinvertebrate communities recorded on days 30 and 60 compared with days 90 to 180. However, in pools and riffles, no temporal variation was observed in the taxonomic composition of macroinvertebrates on all three substrate groups. The trait-based fuzzy correspondence analysis revealed differential spatial-temporal distribution of macroinvertebrate traits on all three substrate group. The early colonisers i.e. day 30 – 60, were dominated by group of taxa characterised by medium (>10 – 20 mm) and large (20 > 40) body size, flat body, collector-gatherers, free-living, and predators. The late colonisers, collected mainly on day 150 and 180 were dominated by taxa with a preference for high flow velocity (0.3 - 0.6 m/s), permanent attachment, and filter-feeding mode. Traits such as oval and flat body shape, medium body size (>10 - 20 mm), skating and clinging/climbing mobility, temporal attachment, shredders, predators, prey, and plastron and spiracle respiration showed positive correlation with the 100% macroplastic substrates. Filter feeding, crawling, permanent attachment, a preference for fast velocity (0.3-0.6 m/s), and coarse particle organic matter were positively correlated with the 50% macroplastic substrates. Overall, the results provided critical insights on the impact of macroplastics on the assemblage structure of biological communities by acting as suitable habitats in stream ecosystems. The study elucidated the role of traits of aquatic organisms in mediating the colonisation of plastics substrates, providing insights into the impact of plastics proliferation on riverine ecosystem functioning. Furthermore, the finding provides a baseline insight into the influence of hydraulic biotopes on the colonisation and establishment of macroinvertebrates on macroplastic acting as artificial riverine habitat. , Thesis (MSc) -- Faculty of Science, Institute for Water Research, 2023
- Full Text:
- Date Issued: 2023-10-13
- Authors: Ali, Andrew Abagai
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/424153 , vital:72128
- Description: Emerging pollutants, such as plastics are threat to freshwater ecosystems, and may negatively impact riverine systems. They can modify riverine habitats and affect aquatic organism distribution and composition. Knowledge of how macroplastics alter riverine habitat heterogeneity, and their effects on macroinvertebrate assemblage structure is sparse, especially in Africa. This study examines the effect of hydraulic biotopes on the colonisation, establishment and succession patterns of macroinvertebrates on macroplastic and natural substrates based on the taxonomic and trait-based approach. Four experimental sites from minimally impacted upper reaches of the Buffalo, Kat, Kowie, and Swartkops Rivers in the Eastern Cape of South Africa were selected for the deployment of plastic substrates. Plastics materials, including polyethylene terephthalate (PET) bottles and natural substrate composed of stone and vegetation, were used to formulate three substrate groups: Group 1: 100% natural substrates (NS), Group 2: 50% natural substrates and 50% plastic material (NP), and Group 3: 100% plastic materials (PD). These substrates were placed in litter bags of equal dimension (25 cm by 35 cm, with 2.5 cm mesh) and deployed randomly in three hydraulic biotopes (pools, riffles, runs) over a period of 180 days (October 2021 to April 2022). A total of 216 substrate bags, 54 bags per substrate were deployed per site in the four experimental sites. Twelve bags from each substrate group were retrieved at an interval of 30 days beginning on day 30 after deployment, and analysed for the establishment of macroinvertebrate communities. Based on composite hydraulic biotope data, Simpson index was significantly higher (P < 0.05) for macroinvertebrate assemblage structure on the 50% and 100% macroplastic substrate groups compared to natural substrates. With the exception of Tabanidae, Glossosomatidae, and Psephenidae, all macroinvertebrate taxa recorded showed non-significant positive correlations with all three substrate groups. However, Tabanidae, Glossosomatidae, and Psephenidae showed significant positive correlation with the 100% natural substrates, 50% plastic substrates and 100% plastic substrates, respectively. The parsimony analysis reveal that, within 30 days, all substrate groups underwent similar succession, with high abundance of pioneer taxa which increased on days 60 and 90, and then decreased from days 120 to 180. For the the pool biotope, Shannon and Simpson indices were significantly higher (P < 0.05) for the macroinvertabrates collected over the natural substates compared with those collected on the macroplastic substrate groups. However, in the riffle and run biotopes, all diversity indices were similar for all substrate groups and no statistically significant difference was observed. Statistically significant higher values for taxonomic richness, diversity, and evenness were found on day 30 to 90 for the riffle biotopes, and day 30 to 60 for the run biotopes. The run biotope presented temporal statistical significant variability in taxonomic composition with different macroinvertebrate communities recorded on days 30 and 60 compared with days 90 to 180. However, in pools and riffles, no temporal variation was observed in the taxonomic composition of macroinvertebrates on all three substrate groups. The trait-based fuzzy correspondence analysis revealed differential spatial-temporal distribution of macroinvertebrate traits on all three substrate group. The early colonisers i.e. day 30 – 60, were dominated by group of taxa characterised by medium (>10 – 20 mm) and large (20 > 40) body size, flat body, collector-gatherers, free-living, and predators. The late colonisers, collected mainly on day 150 and 180 were dominated by taxa with a preference for high flow velocity (0.3 - 0.6 m/s), permanent attachment, and filter-feeding mode. Traits such as oval and flat body shape, medium body size (>10 - 20 mm), skating and clinging/climbing mobility, temporal attachment, shredders, predators, prey, and plastron and spiracle respiration showed positive correlation with the 100% macroplastic substrates. Filter feeding, crawling, permanent attachment, a preference for fast velocity (0.3-0.6 m/s), and coarse particle organic matter were positively correlated with the 50% macroplastic substrates. Overall, the results provided critical insights on the impact of macroplastics on the assemblage structure of biological communities by acting as suitable habitats in stream ecosystems. The study elucidated the role of traits of aquatic organisms in mediating the colonisation of plastics substrates, providing insights into the impact of plastics proliferation on riverine ecosystem functioning. Furthermore, the finding provides a baseline insight into the influence of hydraulic biotopes on the colonisation and establishment of macroinvertebrates on macroplastic acting as artificial riverine habitat. , Thesis (MSc) -- Faculty of Science, Institute for Water Research, 2023
- Full Text:
- Date Issued: 2023-10-13
Maintaining the façade: the disconnect between policy and practice in heritage resources management in Makhanda, South Africa
- Authors: Dlongolo, Zandile Nombulelo
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/424576 , vital:72165
- Description: Cultural heritage is globally acknowledged as having the potential to contribute to positive economic, environmental, political, and social impacts (Graham, 2002). In the South African context, cultural heritage management is rooted in colonial and apartheid narratives that mark a large part of the country's history. Post-apartheid transformation processes have driven new approaches to managing heritage to represent the shared collective narrative of a democratic South Africa. This transformation includes the formation of the three-tiered South African heritage management system by the National Heritage Resources Agency (NHRA) in 1999 and a rethinking of what heritage constitutes and whose heritage matters. Despite the extensive cultural heritage resources in the country, literature concerning the management of these assets in the context of the urban environment and urban planning and management is limited (Donaldson, 2001; Donaldson et al., 2013; Buchanan & Donaldson, 2021; Kruger & Donaldson, 2021). This research explores the built environment heritage resources in Makhanda (formerly Grahamstown) in the Eastern Cape province. Makhanda possesses a rich and varied cultural heritage landscape, including over 70 Provincial Heritage Resources in the form of built environment heritage. The case study provides a perfect laboratory for investigating the various threats and opportunities in the local context that severely affect heritage management. The research used a mixed-method approach to generate data. Primary data were collected through a field survey of built environment heritage resources, semi-structured interviews, and focus groups. Secondary data sources comprised government policy documents, by-laws, reports, research papers and newspaper articles. In the localised context, findings illustrated several challenges affecting local heritage management, centring mainly on the balance between heritage resource management and overall urban management. Findings identified challenges included a poor acknowledgement of the nuances in local history, questions on the effectiveness of legislation, governance and management issues, conflicting demands for social services and urban development, and building maintenance. Heritage resources are acknowledged as a potential tool to meet the local community's needs, and opportunities for developing the heritage sector were also identified. These suggestions include information sharing and cooperation between the municipality, community and various role players, community education, tourism product development, institutional development through skills development, the incorporation of intangible heritage, and the acknowledgement of more inclusive forms of cultural heritage. Overall, the findings indicate that the disconnection in heritage management, urban management practices, and municipal dysfunction in Makhanda threatens the built environment heritage and the local sense of place. The study argues that for local heritage management to succeed, there needs to be a balanced approach to heritage management and urban management through improvements in stakeholder relationships, governance, institutional capacity, knowledge sharing and community involvement in decision-making processes. , Thesis (MSc) -- Faculty of Science, Geography, 2023
- Full Text:
- Date Issued: 2023-10-13
- Authors: Dlongolo, Zandile Nombulelo
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/424576 , vital:72165
- Description: Cultural heritage is globally acknowledged as having the potential to contribute to positive economic, environmental, political, and social impacts (Graham, 2002). In the South African context, cultural heritage management is rooted in colonial and apartheid narratives that mark a large part of the country's history. Post-apartheid transformation processes have driven new approaches to managing heritage to represent the shared collective narrative of a democratic South Africa. This transformation includes the formation of the three-tiered South African heritage management system by the National Heritage Resources Agency (NHRA) in 1999 and a rethinking of what heritage constitutes and whose heritage matters. Despite the extensive cultural heritage resources in the country, literature concerning the management of these assets in the context of the urban environment and urban planning and management is limited (Donaldson, 2001; Donaldson et al., 2013; Buchanan & Donaldson, 2021; Kruger & Donaldson, 2021). This research explores the built environment heritage resources in Makhanda (formerly Grahamstown) in the Eastern Cape province. Makhanda possesses a rich and varied cultural heritage landscape, including over 70 Provincial Heritage Resources in the form of built environment heritage. The case study provides a perfect laboratory for investigating the various threats and opportunities in the local context that severely affect heritage management. The research used a mixed-method approach to generate data. Primary data were collected through a field survey of built environment heritage resources, semi-structured interviews, and focus groups. Secondary data sources comprised government policy documents, by-laws, reports, research papers and newspaper articles. In the localised context, findings illustrated several challenges affecting local heritage management, centring mainly on the balance between heritage resource management and overall urban management. Findings identified challenges included a poor acknowledgement of the nuances in local history, questions on the effectiveness of legislation, governance and management issues, conflicting demands for social services and urban development, and building maintenance. Heritage resources are acknowledged as a potential tool to meet the local community's needs, and opportunities for developing the heritage sector were also identified. These suggestions include information sharing and cooperation between the municipality, community and various role players, community education, tourism product development, institutional development through skills development, the incorporation of intangible heritage, and the acknowledgement of more inclusive forms of cultural heritage. Overall, the findings indicate that the disconnection in heritage management, urban management practices, and municipal dysfunction in Makhanda threatens the built environment heritage and the local sense of place. The study argues that for local heritage management to succeed, there needs to be a balanced approach to heritage management and urban management through improvements in stakeholder relationships, governance, institutional capacity, knowledge sharing and community involvement in decision-making processes. , Thesis (MSc) -- Faculty of Science, Geography, 2023
- Full Text:
- Date Issued: 2023-10-13
Marine plastic pollution impacts on ecosystem services and livelihoods in South Africa: a review and stakeholder perceptions
- Authors: Yose, Papama
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/424743 , vital:72180
- Description: Marine plastic pollution is one of the major environmental problems globally, with adverse impacts on marine ecosystem services, functions and benefits and people. Yet, compared to plastic pollution impacts on terrestrial ecosystems, the impacts of plastic waste on marine ecosystems are little studied and poorly understood in South Africa. In response, research interest on the ecological and economic impacts of marine plastic pollution has grown rapidly but socio-cultural impacts remain poorly conceptualized and little understudied. This trend is arguably driven by the dominance of a quantitative paradigm which is arguably inadequate to respond to dynamic socio-cultural issues and contexts. Further, in South Africa, marine ecosystems are used by diverse groups of people ranging from subsistence users, tourism operators, commercial fishers and recreational users, meaning that addressing marine plastic pollution requires a multi-stakeholder approach. However, few studies explore the perceptions of diverse stakeholders regarding marine plastic pollution, its impacts and potential interventions. Against this background, the objective of the thesis are twofold: (a) to argue for a more nuanced understanding of marine plastic pollution impacts on human well-being in order to strengthen conceptualization of impacts that goes beyond direct and quantitatively measured impacts (ecological, health and economic) but considers the socio-cultural dimensions of impacts (lifestyle disruptions, mental health costs and cultural and heritage impacts), and (b) examine stakeholders’ perceptions of marine plastic pollution, its impacts and potential interventions in South Africa. Drawing on case studies, this study provides a working definition of the socio-cultural impacts of marine plastic pollution and a conceptual framework for categorizing the impacts. It highlights three dimensions of the socio-cultural impacts of marine plastic pollution (lifestyle, mental health, and cultural and heritage impacts). It also illustrates connections between marine ecosystems and marine-based livelihood activities with the social and cultural dimensions of human wellbeing to show the links between marine environments and socio-cultural contexts. Concerning stakeholder perceptions, the study showed a tendency to associate marine plastic pollution with immediate, noticeable and easily quantifiable ecological impacts such as entanglement and ingestion of marine organisms, economic impacts and social impacts (e.g., loss of aesthetic appeal). However, secondary impacts such as loss of marine biodiversity and social impacts such as human health, were largely unknown. Moreover, there was heterogeneity in perceptions of marine plastic pollution impacts among the stakeholder groups. Prospective studies researching marine plastic pollution impacts should consider divergent and diverse perceptions of different stakeholders to capture the real costs of marine plastic pollution. , Thesis (MSc) -- Faculty of Science, Environmental Science, 2023
- Full Text:
- Date Issued: 2023-10-13
- Authors: Yose, Papama
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/424743 , vital:72180
- Description: Marine plastic pollution is one of the major environmental problems globally, with adverse impacts on marine ecosystem services, functions and benefits and people. Yet, compared to plastic pollution impacts on terrestrial ecosystems, the impacts of plastic waste on marine ecosystems are little studied and poorly understood in South Africa. In response, research interest on the ecological and economic impacts of marine plastic pollution has grown rapidly but socio-cultural impacts remain poorly conceptualized and little understudied. This trend is arguably driven by the dominance of a quantitative paradigm which is arguably inadequate to respond to dynamic socio-cultural issues and contexts. Further, in South Africa, marine ecosystems are used by diverse groups of people ranging from subsistence users, tourism operators, commercial fishers and recreational users, meaning that addressing marine plastic pollution requires a multi-stakeholder approach. However, few studies explore the perceptions of diverse stakeholders regarding marine plastic pollution, its impacts and potential interventions. Against this background, the objective of the thesis are twofold: (a) to argue for a more nuanced understanding of marine plastic pollution impacts on human well-being in order to strengthen conceptualization of impacts that goes beyond direct and quantitatively measured impacts (ecological, health and economic) but considers the socio-cultural dimensions of impacts (lifestyle disruptions, mental health costs and cultural and heritage impacts), and (b) examine stakeholders’ perceptions of marine plastic pollution, its impacts and potential interventions in South Africa. Drawing on case studies, this study provides a working definition of the socio-cultural impacts of marine plastic pollution and a conceptual framework for categorizing the impacts. It highlights three dimensions of the socio-cultural impacts of marine plastic pollution (lifestyle, mental health, and cultural and heritage impacts). It also illustrates connections between marine ecosystems and marine-based livelihood activities with the social and cultural dimensions of human wellbeing to show the links between marine environments and socio-cultural contexts. Concerning stakeholder perceptions, the study showed a tendency to associate marine plastic pollution with immediate, noticeable and easily quantifiable ecological impacts such as entanglement and ingestion of marine organisms, economic impacts and social impacts (e.g., loss of aesthetic appeal). However, secondary impacts such as loss of marine biodiversity and social impacts such as human health, were largely unknown. Moreover, there was heterogeneity in perceptions of marine plastic pollution impacts among the stakeholder groups. Prospective studies researching marine plastic pollution impacts should consider divergent and diverse perceptions of different stakeholders to capture the real costs of marine plastic pollution. , Thesis (MSc) -- Faculty of Science, Environmental Science, 2023
- Full Text:
- Date Issued: 2023-10-13
Mechanisms conditioning the implementation of an integrated quality assurance and enhancement approach at a South African University of Technology
- Authors: Mabote, Ntele Emily
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/431576 , vital:72787 , DOI 10.21504/10962/431576
- Description: Literature related to quality in higher education argues that achieving an integrated approach which balances improvement and accountability in a single quality assurance (QA) system, is not easy. In response to the literature, I decided to conduct a realist study to identify mechanisms that can enable or constrain the implementation of an integrated approach in a single quality assurance system at the Tshwane University of Technology (TUT). The scope of my study was limited to teaching and learning as one of the University’s core functions. An integrated approach encouraged a deliberate focus and attention on transformative learning and teaching. The main research question, “what mechanisms enable or constrain the implementation of an integrated approach to quality assurance and enhancement at the Tshwane University of Technology,” underpinned this study. I used Bhaskar’s critical realist philosophy as an underlabourer for the study and Archer’s social realism as an analytical framework to enable me to seek answers to the research questions. The study took the form of a case study at TUT. Data was generated through document analysis and thirty-five semi-structured interviews with agents from across the various levels and campuses of TUT. In keeping with a social realist study, I used Archer’s concept of analytical dualism to analyse structure, culture, and agency separately, and their interplay. My findings indicated that compliance and accountability are related cultural mechanisms and were dominant in the University’s cultural system. This signalled a strong emphasis on quality assurance (QA) rather than quality enhancement (QE). In addition, the findings showed that the University has established sufficient structural and agential enablements to assure the quality of learning and teaching. However, there is a need to integrate transformative cultural mechanisms into the University’s QA system. Furthermore, there were limited structural, cultural, and agential enablements to encourage enhancement. In this regard, I recommended mechanisms that should be in place for an integrated QA and QE approach to be successful at TUT. My main argument is that an institutional context that encourages structural, cultural, and agential QA and QE mechanisms to work in tandem can enable an integrated QA and QE approach. , Thesis (PhD) -- Faculty of Education, Centre for Higher Education Research, Teaching and Learning, 2023
- Full Text:
- Date Issued: 2023-10-13
- Authors: Mabote, Ntele Emily
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/431576 , vital:72787 , DOI 10.21504/10962/431576
- Description: Literature related to quality in higher education argues that achieving an integrated approach which balances improvement and accountability in a single quality assurance (QA) system, is not easy. In response to the literature, I decided to conduct a realist study to identify mechanisms that can enable or constrain the implementation of an integrated approach in a single quality assurance system at the Tshwane University of Technology (TUT). The scope of my study was limited to teaching and learning as one of the University’s core functions. An integrated approach encouraged a deliberate focus and attention on transformative learning and teaching. The main research question, “what mechanisms enable or constrain the implementation of an integrated approach to quality assurance and enhancement at the Tshwane University of Technology,” underpinned this study. I used Bhaskar’s critical realist philosophy as an underlabourer for the study and Archer’s social realism as an analytical framework to enable me to seek answers to the research questions. The study took the form of a case study at TUT. Data was generated through document analysis and thirty-five semi-structured interviews with agents from across the various levels and campuses of TUT. In keeping with a social realist study, I used Archer’s concept of analytical dualism to analyse structure, culture, and agency separately, and their interplay. My findings indicated that compliance and accountability are related cultural mechanisms and were dominant in the University’s cultural system. This signalled a strong emphasis on quality assurance (QA) rather than quality enhancement (QE). In addition, the findings showed that the University has established sufficient structural and agential enablements to assure the quality of learning and teaching. However, there is a need to integrate transformative cultural mechanisms into the University’s QA system. Furthermore, there were limited structural, cultural, and agential enablements to encourage enhancement. In this regard, I recommended mechanisms that should be in place for an integrated QA and QE approach to be successful at TUT. My main argument is that an institutional context that encourages structural, cultural, and agential QA and QE mechanisms to work in tandem can enable an integrated QA and QE approach. , Thesis (PhD) -- Faculty of Education, Centre for Higher Education Research, Teaching and Learning, 2023
- Full Text:
- Date Issued: 2023-10-13
Mega-churches and the neo-Pentecostalisation of South Africa’s black middle class
- Authors: Ngoma, Amuzweni Lerato
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/432422 , vital:72869 , DOI 10.21504/10962/432422
- Description: This thesis argues that the presence and expansion of South African neo-Pentecostal Charismatic Churches (neo-PCCs) and mega-churches holds significant political economy consequences. Methodologically, the thesis is anchored on digital and in-person ethnography, life history and in-depth interviews. The central argument of this thesis is that neo-PCCs and mega-churches are holding spaces for societal change. The symbolic capital of neo-PCCs and mega-churches and the neo-Pentecostalised habitus, which is co-produced by middle-classes acts as a conduit that facilitates social transitions of political and economic orders. Indeed, religion emerges as a transition mechanism as Durkheim argued for France. It has helped South African social groups to extricate themselves from racist discourses, foster non-racialism and build empowered, somewhat deracialised modern middle-class discourses and tastes. White and Black middle classes have co-produced neo-Pentecostal habiti in the post-apartheid era, that have first, built dispositions for neoliberal capital democracy from apartheid capitalism, second as a middle classing and elite making mechanism and field, third as a stabilising, consolidating and upward strategy of social reproduction. In this way, neo-PCCs and the Black middle class have significantly affected the post-apartheid social formation by producing dispositions that uphold financialised neoliberal capitalism. Significantly, accumulated cultural capital is an indispensable resource in initiating and building post-apartheid institutions. As in the neo-PCC field, it has been pastors, prophets and bishops that have demonstrated the capability to accumulate, transubstantiate and maintain cultural capital that has made their churches comparatively durable social institutions. In a political economy context of state-capture and corruption, a post-GFC-and-COVID-19 milieu characterised by the absence of economic growth, rising unemployment, business closure and ever-increasing interest rates that affect indebted middle-class households and the poor alike, the mega-churches studied herein and their neo-Pentecostalised Black middle class adherents expressed an intense dislike for South African politicians across party lines, and especially the poor performance of the African National Congress-dominated state. So that it is possible that mega-churches and their leaders will outlive many new political parties and independents in the same way that they have outlived post-1994 political parties such as the New National Party, Agang South Africa and the Independent Democrats. Much like the buffer Black middle class that was promoted by the apartheid state as a project of reforming apartheid in the 1970s, whose political activism was pragramatically disengaged, this will likely continue, unless if, generally the post-apartheid Black middle classes shift their sociality from in-ward looking-enclaved social anxiety. , Thesis (PhD) -- Faculty of Humanities, Sociology, 2023
- Full Text:
- Date Issued: 2023-10-13
- Authors: Ngoma, Amuzweni Lerato
- Date: 2023-10-13
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/432422 , vital:72869 , DOI 10.21504/10962/432422
- Description: This thesis argues that the presence and expansion of South African neo-Pentecostal Charismatic Churches (neo-PCCs) and mega-churches holds significant political economy consequences. Methodologically, the thesis is anchored on digital and in-person ethnography, life history and in-depth interviews. The central argument of this thesis is that neo-PCCs and mega-churches are holding spaces for societal change. The symbolic capital of neo-PCCs and mega-churches and the neo-Pentecostalised habitus, which is co-produced by middle-classes acts as a conduit that facilitates social transitions of political and economic orders. Indeed, religion emerges as a transition mechanism as Durkheim argued for France. It has helped South African social groups to extricate themselves from racist discourses, foster non-racialism and build empowered, somewhat deracialised modern middle-class discourses and tastes. White and Black middle classes have co-produced neo-Pentecostal habiti in the post-apartheid era, that have first, built dispositions for neoliberal capital democracy from apartheid capitalism, second as a middle classing and elite making mechanism and field, third as a stabilising, consolidating and upward strategy of social reproduction. In this way, neo-PCCs and the Black middle class have significantly affected the post-apartheid social formation by producing dispositions that uphold financialised neoliberal capitalism. Significantly, accumulated cultural capital is an indispensable resource in initiating and building post-apartheid institutions. As in the neo-PCC field, it has been pastors, prophets and bishops that have demonstrated the capability to accumulate, transubstantiate and maintain cultural capital that has made their churches comparatively durable social institutions. In a political economy context of state-capture and corruption, a post-GFC-and-COVID-19 milieu characterised by the absence of economic growth, rising unemployment, business closure and ever-increasing interest rates that affect indebted middle-class households and the poor alike, the mega-churches studied herein and their neo-Pentecostalised Black middle class adherents expressed an intense dislike for South African politicians across party lines, and especially the poor performance of the African National Congress-dominated state. So that it is possible that mega-churches and their leaders will outlive many new political parties and independents in the same way that they have outlived post-1994 political parties such as the New National Party, Agang South Africa and the Independent Democrats. Much like the buffer Black middle class that was promoted by the apartheid state as a project of reforming apartheid in the 1970s, whose political activism was pragramatically disengaged, this will likely continue, unless if, generally the post-apartheid Black middle classes shift their sociality from in-ward looking-enclaved social anxiety. , Thesis (PhD) -- Faculty of Humanities, Sociology, 2023
- Full Text:
- Date Issued: 2023-10-13