A chemo-enzymatic process for the production of beta-thymidine, a key intermediate in antiretrovirol manufacture
- Gordon, Gregory Ernest Robert
- Authors: Gordon, Gregory Ernest Robert
- Date: 2010
- Subjects: HIV infections -- Treatment -- South Africa , HIV infections -- South Africa -- Prevention , Antiretroviral agents
- Language: English
- Type: Thesis , Doctoral , DTech
- Identifier: vital:10423 , http://hdl.handle.net/10948/d1016217
- Description: The socio-economic impact of HIV/AIDS on South Africa has resulted in lower gross domestic product, loss of skills in key sectors such as education, and increased health-care costs in providing access to treatment. Currently active pharmaceutical ingredients (API’s) such as stavudine (d4T) and azidothymidine (AZT) are imported from India and China, while formulation is conducted locally. A strategy was initiated between CSIR Biosciences and LIFElab under the auspices of Arvir Technologies to investigate the feasibility of local antiretroviral manufacture (d4T and AZT) or the manufacture of a key intermediate such as β- thymidine (dT). Several advantages associated with successful implementation of this strategy include ensuring a local supply of API’s, thus reducing reliance on procurement from foreign sources and reducing the effect of foreign exchange rate fluctuations on providing cost effective access to treatment. A local supply source would also reduce the imports and thus aid the balance of payments deficit, and in addition to this, provide stimulus in the local pharmaceutical manufacturing industry (which has been in decline for several decades), resulting in increased skills and employment opportunities. This thesis describes the development of a superior chemo-enzymatic process for the production of β-thymidine (72 percent yield, prior to isolation), a key intermediate in the preparation of anti-retrovirals. Alternative processes based purely on chemical or bioprocess transformations to prepare either 5-methyluridine (5-MU) or dT suffer from several disadvantages: lengthy transformations due to protection/deprotection strategies, low selectivties and product yields (30 percent in the chemical process) and isolation of the product from dilute process streams requiring the use of large uneconomical reactors (bioprocesss). This contributes significantly to the cost of d4T and AZT manufacture. Our novel chemoenzymatic process comprises of a biocatalytic reaction for the production of 5-MU, with subsequent chemical transformation into dT (3 steps) negating and circumventing the limitations of the chemical or bioprocess routes. During the course of this project development, the β-thymidine selling price declined from 175 $/kg (2005) to 100 $/kg (2008). However, the process described in this work is still competitive based on the current β- thymidine selling price of 100 $/kg. The process economics show that with further optimization and increasing the isolated dT yield from 70 percent to 90 percent, the variable cost decreases from 136 $/kg to 110 $/kg. The increase in isolated yield is highly probable, based on solubility data of β-thymidine. The decrease in β-thymidine selling price and technological improvement in dT manufacture should translate into lower API manufacture costs and more cost effective access to treatment. Our novel biocatalytic process producing 5-MU uses a coupled enzyme system employing PNP, Purine Nucleoside Phosphorylase and PyNP, Pyrimidine Nucleoside Phosphorylase. The overall transglycosylation reaction may be decoupled into the phosphorolysis reaction (PNP) and synthesis reaction (PyNP). During the phosphorolysis reaction, guanosine is converted into guanine and ribose-1-phosphate (R-1-P) in the presence of PNP enzyme. The reaction intermediate R-1-P is then coupled to thymine in the presence of PyNP enzyme during the synthesis reaction, producing 5-MU. The process was scaled up from lab-scale to bench-scale (10 - 20 L) and demonstrated to be robust and reproducible. This is evident from the average guanosine conversion (94.7 percent ± 2.03) and 5-MU yield (88.2 percent ± 6.21) and mole balance (104 percent ± 7.61) which were obtained at bench-scale (3 replicates, 10 L). The reaction was carried out at reactor productivities of between 7 – 11 g.L-1.h-1. The integration of the biocatalytic process and chemical processes was successfully carried out, showing that 5-MU produced using our novel biocatalytic process behaved similarly to commercially available 5- MU (ex. Dayang Chemicals, China). A PCT patent application (Ref. No. P44422PC01) on this chemo-enzymatic process has been filed and currently public private partnerships are being explored through Arvir Technologies to evaluate and validate this technology at one ton scale.
- Full Text:
- Date Issued: 2010
- Authors: Gordon, Gregory Ernest Robert
- Date: 2010
- Subjects: HIV infections -- Treatment -- South Africa , HIV infections -- South Africa -- Prevention , Antiretroviral agents
- Language: English
- Type: Thesis , Doctoral , DTech
- Identifier: vital:10423 , http://hdl.handle.net/10948/d1016217
- Description: The socio-economic impact of HIV/AIDS on South Africa has resulted in lower gross domestic product, loss of skills in key sectors such as education, and increased health-care costs in providing access to treatment. Currently active pharmaceutical ingredients (API’s) such as stavudine (d4T) and azidothymidine (AZT) are imported from India and China, while formulation is conducted locally. A strategy was initiated between CSIR Biosciences and LIFElab under the auspices of Arvir Technologies to investigate the feasibility of local antiretroviral manufacture (d4T and AZT) or the manufacture of a key intermediate such as β- thymidine (dT). Several advantages associated with successful implementation of this strategy include ensuring a local supply of API’s, thus reducing reliance on procurement from foreign sources and reducing the effect of foreign exchange rate fluctuations on providing cost effective access to treatment. A local supply source would also reduce the imports and thus aid the balance of payments deficit, and in addition to this, provide stimulus in the local pharmaceutical manufacturing industry (which has been in decline for several decades), resulting in increased skills and employment opportunities. This thesis describes the development of a superior chemo-enzymatic process for the production of β-thymidine (72 percent yield, prior to isolation), a key intermediate in the preparation of anti-retrovirals. Alternative processes based purely on chemical or bioprocess transformations to prepare either 5-methyluridine (5-MU) or dT suffer from several disadvantages: lengthy transformations due to protection/deprotection strategies, low selectivties and product yields (30 percent in the chemical process) and isolation of the product from dilute process streams requiring the use of large uneconomical reactors (bioprocesss). This contributes significantly to the cost of d4T and AZT manufacture. Our novel chemoenzymatic process comprises of a biocatalytic reaction for the production of 5-MU, with subsequent chemical transformation into dT (3 steps) negating and circumventing the limitations of the chemical or bioprocess routes. During the course of this project development, the β-thymidine selling price declined from 175 $/kg (2005) to 100 $/kg (2008). However, the process described in this work is still competitive based on the current β- thymidine selling price of 100 $/kg. The process economics show that with further optimization and increasing the isolated dT yield from 70 percent to 90 percent, the variable cost decreases from 136 $/kg to 110 $/kg. The increase in isolated yield is highly probable, based on solubility data of β-thymidine. The decrease in β-thymidine selling price and technological improvement in dT manufacture should translate into lower API manufacture costs and more cost effective access to treatment. Our novel biocatalytic process producing 5-MU uses a coupled enzyme system employing PNP, Purine Nucleoside Phosphorylase and PyNP, Pyrimidine Nucleoside Phosphorylase. The overall transglycosylation reaction may be decoupled into the phosphorolysis reaction (PNP) and synthesis reaction (PyNP). During the phosphorolysis reaction, guanosine is converted into guanine and ribose-1-phosphate (R-1-P) in the presence of PNP enzyme. The reaction intermediate R-1-P is then coupled to thymine in the presence of PyNP enzyme during the synthesis reaction, producing 5-MU. The process was scaled up from lab-scale to bench-scale (10 - 20 L) and demonstrated to be robust and reproducible. This is evident from the average guanosine conversion (94.7 percent ± 2.03) and 5-MU yield (88.2 percent ± 6.21) and mole balance (104 percent ± 7.61) which were obtained at bench-scale (3 replicates, 10 L). The reaction was carried out at reactor productivities of between 7 – 11 g.L-1.h-1. The integration of the biocatalytic process and chemical processes was successfully carried out, showing that 5-MU produced using our novel biocatalytic process behaved similarly to commercially available 5- MU (ex. Dayang Chemicals, China). A PCT patent application (Ref. No. P44422PC01) on this chemo-enzymatic process has been filed and currently public private partnerships are being explored through Arvir Technologies to evaluate and validate this technology at one ton scale.
- Full Text:
- Date Issued: 2010
A combination of platinum anticancer drugs and mangiferin causes increased efficacy in cancer cell lines
- Authors: Du Plessis-Stoman, Debbie
- Date: 2010
- Subjects: Cancer -- Chemotherapy , Antineoplastic agents , Platinum compounds -- Therapeutic use , Cancer cells
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:10338 , http://hdl.handle.net/10948/d1016160
- Description: This thesis mainly deals with some biochemical aspects regarding the efficacy of novel platinum anticancer compounds alone and in combination with mangiferin, as part of a broader study in which both chemistry and biochemistry are involved. Various novel diamine and N-S donor chelate compounds of platinum II and IV have been developed in which factors such as stereochemistry, ligand exchange rate and biocompatibility were considered as additional parameters. In the first order testing, each of these compounds was tested with reference to their “killing” potential by comparing their rate of killing, over a period of 48 hours with those of cisplatin and oxaliplatin. Numerous novel compounds were tested in this way, using the MTT cell viability assay and the three cancer cell lines MCF7, HT29 and HeLa. Although only a few could be regarded as equal to or even better than cisplatin, CPA7 and oxaliplatin, the testing of these compounds on cancer cells provided useful knowledge for the further development of novel compounds. Three of the better compounds, namely Yol 25, Yol 29.1 and Mar 4.1.4 were selected for further studies, together with oxaliplatin and CPA7 as positive controls, to obtain more detailed knowledge of their anticancer action, both alone and when applied in combination with mangiferin. In addition to the above, resistant cells were produced for each of the three different cell lines tested and all the selected compounds, both in the presence and absence of mangiferin. The effects of these treatments on the activation of NFĸB when applied to normal and resistant cell lines were also investigated. All the compounds induced apoptosis in the cell lines tested as well as alter the DNA cycle at one or more phase. Additionally, combination of these compounds with mangiferin enhanced the above-mentioned effects. Mangiferin decreases the IC50 values of the platinum drugs by up to 3.4 times and, although mangiferin alone did not induce cell cycle arrest, the presence of mangiferin in combination with oxaliplatin and Yol 25 shows an earlier and greatly enhanced delay in the S-phase, while cells treated with CPA7, Yol 29.1 and Mar 4.1.4 in combination with mangiferin showed a later, but greatly enhanced delay in the S-phase. It was also found that mangiferin acts as an NFĸB inhibitor when applied in combination with these drugs, which, in turn, reduces the occurrence of resistance in the cell lines. Resistance to oxaliplatin was counteracted by the combination with mangiferin in HeLa and HT29, but not in MCF7 cells, while resistance to CPA7 was only counteracted in the MCF7 cell line. Yol 25 and Mar 4.1.4 did not seem to induce resistance in HeLa and MCF7 cells, but did in HT29 cells, whereas Yol 29.1 caused resistance in HeLa and HT29 cells, but not in MCF7 cells. Finally, an effort was made to evaluate the different compounds by comparing them with respect to their properties relating to anticancer action with and without the addition of mangiferin.
- Full Text:
- Date Issued: 2010
- Authors: Du Plessis-Stoman, Debbie
- Date: 2010
- Subjects: Cancer -- Chemotherapy , Antineoplastic agents , Platinum compounds -- Therapeutic use , Cancer cells
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:10338 , http://hdl.handle.net/10948/d1016160
- Description: This thesis mainly deals with some biochemical aspects regarding the efficacy of novel platinum anticancer compounds alone and in combination with mangiferin, as part of a broader study in which both chemistry and biochemistry are involved. Various novel diamine and N-S donor chelate compounds of platinum II and IV have been developed in which factors such as stereochemistry, ligand exchange rate and biocompatibility were considered as additional parameters. In the first order testing, each of these compounds was tested with reference to their “killing” potential by comparing their rate of killing, over a period of 48 hours with those of cisplatin and oxaliplatin. Numerous novel compounds were tested in this way, using the MTT cell viability assay and the three cancer cell lines MCF7, HT29 and HeLa. Although only a few could be regarded as equal to or even better than cisplatin, CPA7 and oxaliplatin, the testing of these compounds on cancer cells provided useful knowledge for the further development of novel compounds. Three of the better compounds, namely Yol 25, Yol 29.1 and Mar 4.1.4 were selected for further studies, together with oxaliplatin and CPA7 as positive controls, to obtain more detailed knowledge of their anticancer action, both alone and when applied in combination with mangiferin. In addition to the above, resistant cells were produced for each of the three different cell lines tested and all the selected compounds, both in the presence and absence of mangiferin. The effects of these treatments on the activation of NFĸB when applied to normal and resistant cell lines were also investigated. All the compounds induced apoptosis in the cell lines tested as well as alter the DNA cycle at one or more phase. Additionally, combination of these compounds with mangiferin enhanced the above-mentioned effects. Mangiferin decreases the IC50 values of the platinum drugs by up to 3.4 times and, although mangiferin alone did not induce cell cycle arrest, the presence of mangiferin in combination with oxaliplatin and Yol 25 shows an earlier and greatly enhanced delay in the S-phase, while cells treated with CPA7, Yol 29.1 and Mar 4.1.4 in combination with mangiferin showed a later, but greatly enhanced delay in the S-phase. It was also found that mangiferin acts as an NFĸB inhibitor when applied in combination with these drugs, which, in turn, reduces the occurrence of resistance in the cell lines. Resistance to oxaliplatin was counteracted by the combination with mangiferin in HeLa and HT29, but not in MCF7 cells, while resistance to CPA7 was only counteracted in the MCF7 cell line. Yol 25 and Mar 4.1.4 did not seem to induce resistance in HeLa and MCF7 cells, but did in HT29 cells, whereas Yol 29.1 caused resistance in HeLa and HT29 cells, but not in MCF7 cells. Finally, an effort was made to evaluate the different compounds by comparing them with respect to their properties relating to anticancer action with and without the addition of mangiferin.
- Full Text:
- Date Issued: 2010
A computing studio method for teaching design thinking
- Authors: Van der Post, Leda
- Date: 2010
- Subjects: Creative thinking -- Study and teaching , Creative ability -- Study and teaching , Management information systems
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:10465 , http://hdl.handle.net/10948/1128 , Creative thinking -- Study and teaching , Creative ability -- Study and teaching , Management information systems
- Description: Current trends in technology have led to a need for creativity and innovation in the design of contemporary digital products and systems. This has resulted in design thinking and creative design process having a higher profile in digital design practice. In turn, this has impacted computing education, by creating a need for computing students to develop creativity and design thinking skills. Creative design is taught using design studio teaching methods, which require a culture, environment and activities that are different to the teaching methods used in traditional computing education. Some computing academics have implemented courses using aspects of studio teaching methods, but no clear guidelines for a computing academic without creative design experience to fully apply studio teaching methods in courses could be found. The reason for the change in the role of design in the digital domain and how it affected the needs of computing students was investigated and a comparison of typical design studio and computing teaching methods was conducted using a learning systems model adapted for this specific purpose. This led to an identification of areas that required further investigation, or gaps in the knowledge of how to adapt design studio methods for use within a traditional computing education environment. These gaps were used as the basis for identifying a set of research questions for an empirical study. An exploratory case study was conducted at the Nelson Mandela Metropolitan University to answer the research questions. Three computing academics implemented studio teaching methods within three separate modules, following provisional guidelines devised from the preliminary research. Feedback was collected from the lecturers and the students registered for the modules regarding their experiences. It was found that it would be possible for computing academics to implement aspects of a design studio approach within the existing computing environment. An explicit teaching method, termed the computing studio teaching method, was developed from the results of the case study. This teaching method aims to provide computing academics with little or no creative design experience with explicit methods for implementing modified design studio teaching methods in order to promote creativity and design thinking within traditional computing modules. This teaching method will be tested, validated and refined in future research.
- Full Text:
- Date Issued: 2010
- Authors: Van der Post, Leda
- Date: 2010
- Subjects: Creative thinking -- Study and teaching , Creative ability -- Study and teaching , Management information systems
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:10465 , http://hdl.handle.net/10948/1128 , Creative thinking -- Study and teaching , Creative ability -- Study and teaching , Management information systems
- Description: Current trends in technology have led to a need for creativity and innovation in the design of contemporary digital products and systems. This has resulted in design thinking and creative design process having a higher profile in digital design practice. In turn, this has impacted computing education, by creating a need for computing students to develop creativity and design thinking skills. Creative design is taught using design studio teaching methods, which require a culture, environment and activities that are different to the teaching methods used in traditional computing education. Some computing academics have implemented courses using aspects of studio teaching methods, but no clear guidelines for a computing academic without creative design experience to fully apply studio teaching methods in courses could be found. The reason for the change in the role of design in the digital domain and how it affected the needs of computing students was investigated and a comparison of typical design studio and computing teaching methods was conducted using a learning systems model adapted for this specific purpose. This led to an identification of areas that required further investigation, or gaps in the knowledge of how to adapt design studio methods for use within a traditional computing education environment. These gaps were used as the basis for identifying a set of research questions for an empirical study. An exploratory case study was conducted at the Nelson Mandela Metropolitan University to answer the research questions. Three computing academics implemented studio teaching methods within three separate modules, following provisional guidelines devised from the preliminary research. Feedback was collected from the lecturers and the students registered for the modules regarding their experiences. It was found that it would be possible for computing academics to implement aspects of a design studio approach within the existing computing environment. An explicit teaching method, termed the computing studio teaching method, was developed from the results of the case study. This teaching method aims to provide computing academics with little or no creative design experience with explicit methods for implementing modified design studio teaching methods in order to promote creativity and design thinking within traditional computing modules. This teaching method will be tested, validated and refined in future research.
- Full Text:
- Date Issued: 2010
A framework for mobile SOA using compression
- Authors: Saunders, Evan
- Date: 2010
- Subjects: Service-oriented architecture (Computer science)
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10459 , http://hdl.handle.net/10948/1500 , Service-oriented architecture (Computer science)
- Description: The widely accepted standards of Service-Oriented Architecture (SOA) have changed the way many organisations conduct their everyday business. The significant popularity of mobile devices has seen a rapid increase in the rate of mobile technology enhancements, which have become widely used for communication, as well as conducting everyday tasks. An increased requirement in many businesses is for staff not to be tied down to the office. Consequently, mobile devices play an important role in achieving the mobility and information access that people desire. Due to the popularity and increasing use of SOA and mobile devices, Mobile Service-Oriented Architecture (Mobile SOA) has become a new industry catch-phrase. Many challenges, however, exist within the Mobile SOA environment. These issues include limitations on mobile devices, such as a reduced screen size, lack of processing power, insufficient processing memory, limited battery life, poor storage capacity, unreliable network connections, limited bandwidth available and high transfer costs. This research aimed to provide an elegant solution to the issues of a mobile device, which hinders the performance of Mobile SOA. The main objective of this research was to improve the effectiveness and efficiency of Mobile SOA. In order to achieve this goal, a framework was proposed, which supported intelligent compression of files used within a Web Service. The proposed framework provided a set of guidelines that facilitate the quick development of a system. A proof-of-concept prototype was developed, based on these guidelines and the framework design principles. The prototype provided practical evidence of the effectiveness of implementing a system based on the proposed framework. An analytical evaluation was conducted to determine the effectiveness of the prototype within the Mobile SOA environment. A performance evaluation was conducted to determine efficiency it provides. Additionally, the performance evaluation highlighted the decrease in file transfer time, as well as the significant reduction in transfer costs. The analytical and performance evaluations demonstrated that the prototype optimises the effectiveness and efficiency of Mobile SOA. The framework could, thus, be used to facilitate efficient file transfer between a Server and (Mobile) Client.
- Full Text:
- Date Issued: 2010
- Authors: Saunders, Evan
- Date: 2010
- Subjects: Service-oriented architecture (Computer science)
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10459 , http://hdl.handle.net/10948/1500 , Service-oriented architecture (Computer science)
- Description: The widely accepted standards of Service-Oriented Architecture (SOA) have changed the way many organisations conduct their everyday business. The significant popularity of mobile devices has seen a rapid increase in the rate of mobile technology enhancements, which have become widely used for communication, as well as conducting everyday tasks. An increased requirement in many businesses is for staff not to be tied down to the office. Consequently, mobile devices play an important role in achieving the mobility and information access that people desire. Due to the popularity and increasing use of SOA and mobile devices, Mobile Service-Oriented Architecture (Mobile SOA) has become a new industry catch-phrase. Many challenges, however, exist within the Mobile SOA environment. These issues include limitations on mobile devices, such as a reduced screen size, lack of processing power, insufficient processing memory, limited battery life, poor storage capacity, unreliable network connections, limited bandwidth available and high transfer costs. This research aimed to provide an elegant solution to the issues of a mobile device, which hinders the performance of Mobile SOA. The main objective of this research was to improve the effectiveness and efficiency of Mobile SOA. In order to achieve this goal, a framework was proposed, which supported intelligent compression of files used within a Web Service. The proposed framework provided a set of guidelines that facilitate the quick development of a system. A proof-of-concept prototype was developed, based on these guidelines and the framework design principles. The prototype provided practical evidence of the effectiveness of implementing a system based on the proposed framework. An analytical evaluation was conducted to determine the effectiveness of the prototype within the Mobile SOA environment. A performance evaluation was conducted to determine efficiency it provides. Additionally, the performance evaluation highlighted the decrease in file transfer time, as well as the significant reduction in transfer costs. The analytical and performance evaluations demonstrated that the prototype optimises the effectiveness and efficiency of Mobile SOA. The framework could, thus, be used to facilitate efficient file transfer between a Server and (Mobile) Client.
- Full Text:
- Date Issued: 2010
Acacia Mearnsii debarking: comparing different debarking technologies in the KwaZulu-Natal and Mpumalanga forestry regions of South Africa
- Authors: Eggers, John Rolf
- Date: 2010
- Subjects: Acacia mearnsii -- South Africa -- KwaZulu-Natal , Acacia mearnsii -- South Africa -- Mpumalanga , Wattles (Plants) -- South Africa -- KwaZulu-Natal , Wattles (Plants) -- South Africa -- Mpumalanga
- Language: English
- Type: Thesis , Masters , MTech
- Identifier: vital:10740 , http://hdl.handle.net/10948/1456 , Acacia mearnsii -- South Africa -- KwaZulu-Natal , Acacia mearnsii -- South Africa -- Mpumalanga , Wattles (Plants) -- South Africa -- KwaZulu-Natal , Wattles (Plants) -- South Africa -- Mpumalanga
- Description: Debarking of Acacia mearnsii in South Africa has been conducted using mainly manual systems. Labour shortages and reliability of these systems has caused interest in alternative systems that might be able to debark A. mearnsii. The aim of the research is to compare three mechanised debarking technologies used to debark A. mearnsii. Research trials were conducted on the Demuth, Hyena and Hypro debarkers that would form part of semi-mechanised harvesting systems. The debarking technologies were analysed and compared in terms of productivity per productive machine hour (PMH), debarking quality, system costs and the quality of the bark that was produced. Furthermore, the effect that tree volume, strippability and form have on each of the debarking technologies was determined. The debarking technologies were affected by each of the factors researched. Decreasing tree form had a negative effect on the productivity of each of the technologies. An increase in strippability class (strengthening wood-bark bond strength) caused a decrease in the productivity of each of the machines. An increasing tree volume had a positive effect on the productivity for the three debarking technologies. After the debarking had taken place, samples of bark were analysed at a laboratory to determine if it was acceptable for the processing facilities. Each of the debarking technologies produced bark of varying dimensions, but they were all found to be acceptable. The system costs of the three debarking technologies were compared to a manual system cost at tree volumes of 0.1m³, 0.15m³ and 0.2m³. The Demuth debarker had the best system cost for a tree size of 0.1m³, while the manual system was best for tree volumes 0.15m³ and 0.2m³. The Demuth, Hyena and manual system costs were very similar for each of the tree volumes, with the Hypro being a little higher.
- Full Text:
- Date Issued: 2010
- Authors: Eggers, John Rolf
- Date: 2010
- Subjects: Acacia mearnsii -- South Africa -- KwaZulu-Natal , Acacia mearnsii -- South Africa -- Mpumalanga , Wattles (Plants) -- South Africa -- KwaZulu-Natal , Wattles (Plants) -- South Africa -- Mpumalanga
- Language: English
- Type: Thesis , Masters , MTech
- Identifier: vital:10740 , http://hdl.handle.net/10948/1456 , Acacia mearnsii -- South Africa -- KwaZulu-Natal , Acacia mearnsii -- South Africa -- Mpumalanga , Wattles (Plants) -- South Africa -- KwaZulu-Natal , Wattles (Plants) -- South Africa -- Mpumalanga
- Description: Debarking of Acacia mearnsii in South Africa has been conducted using mainly manual systems. Labour shortages and reliability of these systems has caused interest in alternative systems that might be able to debark A. mearnsii. The aim of the research is to compare three mechanised debarking technologies used to debark A. mearnsii. Research trials were conducted on the Demuth, Hyena and Hypro debarkers that would form part of semi-mechanised harvesting systems. The debarking technologies were analysed and compared in terms of productivity per productive machine hour (PMH), debarking quality, system costs and the quality of the bark that was produced. Furthermore, the effect that tree volume, strippability and form have on each of the debarking technologies was determined. The debarking technologies were affected by each of the factors researched. Decreasing tree form had a negative effect on the productivity of each of the technologies. An increase in strippability class (strengthening wood-bark bond strength) caused a decrease in the productivity of each of the machines. An increasing tree volume had a positive effect on the productivity for the three debarking technologies. After the debarking had taken place, samples of bark were analysed at a laboratory to determine if it was acceptable for the processing facilities. Each of the debarking technologies produced bark of varying dimensions, but they were all found to be acceptable. The system costs of the three debarking technologies were compared to a manual system cost at tree volumes of 0.1m³, 0.15m³ and 0.2m³. The Demuth debarker had the best system cost for a tree size of 0.1m³, while the manual system was best for tree volumes 0.15m³ and 0.2m³. The Demuth, Hyena and manual system costs were very similar for each of the tree volumes, with the Hypro being a little higher.
- Full Text:
- Date Issued: 2010
Aerial optical fibres in telecommunication systems : SOP and PMD monitoring, and tolerance of modulation formats
- Authors: Ireeta, Winston Tumps
- Date: 2010
- Subjects: Optical communications , Fiber optics
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:10542 , http://hdl.handle.net/10948/1478 , http://hdl.handle.net/10948/d1012894 , Optical communications , Fiber optics
- Description: The topic of this thesis is aerial optical fibres in telecommunication systems: state of polarization (SOP) and polarization mode dispersion (PMD) monitoring and tolerance of modulation formats. Errors in optical fibre telecommunication systems are introduced when these polarization effects (SOP and PMD) change. These changes are so intense especially in aerial optical fibres. Part of the backbone of South Africa’s national grid includes long distances of aerial optical fibre between transmission exchange stations. The work in this thesis can be divided into three parts which all deal with the major aspects of PMD in deployed aerial optical fibres: characterization, environmental effects plus other perturbations, and tolerance of different modulation formats. In our work, SOP and PMD field measurements revealed that they both fluctuate more rapidly in deployed aerial optical fibres especially on windy and hot days. The SOP and PMD changes in the aerial optical fibres showed a significant correlation with these environmental parameters. SOP and PMD are stochastic in nature due to changes in the properties of the optical fibres and its positions because of both intrinsic and extrinsic perturbations. In our work, with only 184 PMD values measured and obtained by use of the FTB-5700 single-ended dispersion analyzer, the predicted theoretical Gaussian fit was obtained with a mean of 0.47 ps and standard deviation of 0.08 ps. This small standard deviation was justification for its robustness and accuracy. The statistical distributions for first-order polarization mode dispersion (FO-PMD) and second-order polarization mode dispersion (SO-PMD) for the first time were experimentally confirmed when measured using the FTB-5700 single-ended dispersion analyzer instrument for deployed aerial optical fibres. We were also able to determine the time scale over which to compensate FO-PMD in deployed aerial fibres using the directional time drift autocorrelation function method. It is slightly higher than 390 s for SOP measurements made on a particular windy and hot day. This is due to the fact that the changes of the PMD vector are known to be slower than the SOP changes. vi We also investigated the theoretical statistical distribution that corresponds to output SOP variations. The SOP variations can either be with wavelength (for buried fibre) or with time (for aerial fibre). Our results showed that the statistics of the relative SOP changes approached the distribution proposed by Foschini et al. (2000). Advanced optical modulation formats have become a key ingredient in the design of modern state-of-the-art wavelength-division-multiplexed (WDM) optical transmission systems. In our work, we investigated which of these advanced modulation formats is best suited for the South African network especially on systems that have links of aerial optical fibres. Keywords: aerial optical fibre, polarization mode dispersion (PMD), principal states of polarization (PSP), state of polarization (SOP), first-order PMD, second-order PMD.
- Full Text:
- Date Issued: 2010
- Authors: Ireeta, Winston Tumps
- Date: 2010
- Subjects: Optical communications , Fiber optics
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:10542 , http://hdl.handle.net/10948/1478 , http://hdl.handle.net/10948/d1012894 , Optical communications , Fiber optics
- Description: The topic of this thesis is aerial optical fibres in telecommunication systems: state of polarization (SOP) and polarization mode dispersion (PMD) monitoring and tolerance of modulation formats. Errors in optical fibre telecommunication systems are introduced when these polarization effects (SOP and PMD) change. These changes are so intense especially in aerial optical fibres. Part of the backbone of South Africa’s national grid includes long distances of aerial optical fibre between transmission exchange stations. The work in this thesis can be divided into three parts which all deal with the major aspects of PMD in deployed aerial optical fibres: characterization, environmental effects plus other perturbations, and tolerance of different modulation formats. In our work, SOP and PMD field measurements revealed that they both fluctuate more rapidly in deployed aerial optical fibres especially on windy and hot days. The SOP and PMD changes in the aerial optical fibres showed a significant correlation with these environmental parameters. SOP and PMD are stochastic in nature due to changes in the properties of the optical fibres and its positions because of both intrinsic and extrinsic perturbations. In our work, with only 184 PMD values measured and obtained by use of the FTB-5700 single-ended dispersion analyzer, the predicted theoretical Gaussian fit was obtained with a mean of 0.47 ps and standard deviation of 0.08 ps. This small standard deviation was justification for its robustness and accuracy. The statistical distributions for first-order polarization mode dispersion (FO-PMD) and second-order polarization mode dispersion (SO-PMD) for the first time were experimentally confirmed when measured using the FTB-5700 single-ended dispersion analyzer instrument for deployed aerial optical fibres. We were also able to determine the time scale over which to compensate FO-PMD in deployed aerial fibres using the directional time drift autocorrelation function method. It is slightly higher than 390 s for SOP measurements made on a particular windy and hot day. This is due to the fact that the changes of the PMD vector are known to be slower than the SOP changes. vi We also investigated the theoretical statistical distribution that corresponds to output SOP variations. The SOP variations can either be with wavelength (for buried fibre) or with time (for aerial fibre). Our results showed that the statistics of the relative SOP changes approached the distribution proposed by Foschini et al. (2000). Advanced optical modulation formats have become a key ingredient in the design of modern state-of-the-art wavelength-division-multiplexed (WDM) optical transmission systems. In our work, we investigated which of these advanced modulation formats is best suited for the South African network especially on systems that have links of aerial optical fibres. Keywords: aerial optical fibre, polarization mode dispersion (PMD), principal states of polarization (PSP), state of polarization (SOP), first-order PMD, second-order PMD.
- Full Text:
- Date Issued: 2010
An investigation of IgE regulation by recombinant soluble IgE receptors and co-receptors in human cell culture models
- Authors: Bowles, Sandra Lyn
- Date: 2010
- Subjects: Immunoglobulin E , Allergy
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:10321 , http://hdl.handle.net/10948/1231 , Immunoglobulin E , Allergy
- Description: Type I hypersensitivities are mediated by the IgE antibody. The effector functions and synthesis of IgE result from interactions with a network of proteins that include a high affinity (FcRIα) and a low affinity (CD23, FcRII) Fc receptor in conjunction with the B lymphocyte receptor, CD21. CD23 is a multifunctional type II transmembrane protein that binds its known ligands through its ectodomain either as a membrane-bound or soluble receptor generated in vivo by specific proteolytic cleavages. IgE production is primarily regulated by interactions between IgE, CD23 and CD21. Despite its importance for development of strategies to limit hypersensitivity, precise information about the molecular interactions remains limited. During this study, I engineered, expressed and purified from bacteria three soluble human CD23 fragments that are normally formed in vivo and shed from the cell surface (1) derCD23, amino acids 156-298 (2) sCD23, amino acids 150-321 and (3) the entire ectodomain, exCD23, amino acids 48-321 to examine the comparative binding of recombinant human CD21 SCR 1-2 and native human IgE to these fragments. Gel filtration HPLC revealed that derCD23 and sCD23 were monomeric whereas exCD23 assembled as a heterogeneous mixture that included trimers and monomers. At the concentrations utilized, CD23 fragments sCD23 and exCD23 bound CD21 with similar affinity, whereas interaction between derCD23 and CD21 was minimal when analyzed by surface plasmon resonance (SPR) spectroscopy. These findings suggest that penultimate “tail” amino acids between 298 and 321 stabilize CD21 attachment, although it cannot be ruled out, the region between Met 150 and Ser 156 may also play a role in binding CD21 SCR 1-2. In contrast, there is a progressive increment in the affinity of soluble fragments (exCD23>sCD23>derCD23) for IgE, upon increasing length of the proximal CD23 “stalk” domain. These findings highlight the differences in both the structural basis and affinity of the three physiological fragments of human CD23 for the ligands CD21 and IgE and underscore the complexity of CD23-mediated regulatory networks. It was found that B-cells only make up ~5% of the PBMC population, and that these cells were able to be activated, via STAT-6 phosphorylation, to enter class switch recombination (CSR) by the addition of switch factors (IL-4 and anti-CD40). Titration experiments dictated that 25 ng/mL of CD23 was the most efficient concentration to up-regulate IgE synthesis in PBMCs; furthermore, soluble CD23 proteins were incubated with PBMCs in the presence and absence of CD21 SCR 1-2 to investigate the effect that these recombinant proteins have on IgE synthesis. Results showed that the influence of recombinant proteins (both CD23 and CD21) on IgE synthesis was slight. It was shown that while derCD23 had no significant effect, monomeric sCD23 down-regulated, and the mixture of monomeric and oligomeric exCD23 up-regulated IgE synthesis. On addition of CD21 SCR 1-2 to the cells switched and treated with soluble CD23, it was found that in both cases for sCD23 and exCD23, IgE synthesis was increased, while for derCD23, there was no noticeable difference in IgE synthesis. This confirmed previous data showing the lack of binding between derCD23 and CD21 SCR 1-2. The exact binding site for CD21 on the CD23 molecule is unknown, and incompletely represented in the NMR and crystal structures. It is thought that CD21 binds to the C-terminal tail section, not present in derCD23. It is therefore likely that only a negative-feedback mechanism operates with derCD23 to regulate IgE synthesis. Further investigation of the binding of CD23 fragments to SCR 5-8 of CD21 and the effect of this on IgE synthesis may lead to a potential therapeutic role for derCD23 in the treatment of allergic disease. Data accumulated in this study suggests that investigating the modulation of oligomeric state and thus the activity of soluble CD23 fragments may be important in the construction of new regulators of IgE synthesis.
- Full Text:
- Date Issued: 2010
- Authors: Bowles, Sandra Lyn
- Date: 2010
- Subjects: Immunoglobulin E , Allergy
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:10321 , http://hdl.handle.net/10948/1231 , Immunoglobulin E , Allergy
- Description: Type I hypersensitivities are mediated by the IgE antibody. The effector functions and synthesis of IgE result from interactions with a network of proteins that include a high affinity (FcRIα) and a low affinity (CD23, FcRII) Fc receptor in conjunction with the B lymphocyte receptor, CD21. CD23 is a multifunctional type II transmembrane protein that binds its known ligands through its ectodomain either as a membrane-bound or soluble receptor generated in vivo by specific proteolytic cleavages. IgE production is primarily regulated by interactions between IgE, CD23 and CD21. Despite its importance for development of strategies to limit hypersensitivity, precise information about the molecular interactions remains limited. During this study, I engineered, expressed and purified from bacteria three soluble human CD23 fragments that are normally formed in vivo and shed from the cell surface (1) derCD23, amino acids 156-298 (2) sCD23, amino acids 150-321 and (3) the entire ectodomain, exCD23, amino acids 48-321 to examine the comparative binding of recombinant human CD21 SCR 1-2 and native human IgE to these fragments. Gel filtration HPLC revealed that derCD23 and sCD23 were monomeric whereas exCD23 assembled as a heterogeneous mixture that included trimers and monomers. At the concentrations utilized, CD23 fragments sCD23 and exCD23 bound CD21 with similar affinity, whereas interaction between derCD23 and CD21 was minimal when analyzed by surface plasmon resonance (SPR) spectroscopy. These findings suggest that penultimate “tail” amino acids between 298 and 321 stabilize CD21 attachment, although it cannot be ruled out, the region between Met 150 and Ser 156 may also play a role in binding CD21 SCR 1-2. In contrast, there is a progressive increment in the affinity of soluble fragments (exCD23>sCD23>derCD23) for IgE, upon increasing length of the proximal CD23 “stalk” domain. These findings highlight the differences in both the structural basis and affinity of the three physiological fragments of human CD23 for the ligands CD21 and IgE and underscore the complexity of CD23-mediated regulatory networks. It was found that B-cells only make up ~5% of the PBMC population, and that these cells were able to be activated, via STAT-6 phosphorylation, to enter class switch recombination (CSR) by the addition of switch factors (IL-4 and anti-CD40). Titration experiments dictated that 25 ng/mL of CD23 was the most efficient concentration to up-regulate IgE synthesis in PBMCs; furthermore, soluble CD23 proteins were incubated with PBMCs in the presence and absence of CD21 SCR 1-2 to investigate the effect that these recombinant proteins have on IgE synthesis. Results showed that the influence of recombinant proteins (both CD23 and CD21) on IgE synthesis was slight. It was shown that while derCD23 had no significant effect, monomeric sCD23 down-regulated, and the mixture of monomeric and oligomeric exCD23 up-regulated IgE synthesis. On addition of CD21 SCR 1-2 to the cells switched and treated with soluble CD23, it was found that in both cases for sCD23 and exCD23, IgE synthesis was increased, while for derCD23, there was no noticeable difference in IgE synthesis. This confirmed previous data showing the lack of binding between derCD23 and CD21 SCR 1-2. The exact binding site for CD21 on the CD23 molecule is unknown, and incompletely represented in the NMR and crystal structures. It is thought that CD21 binds to the C-terminal tail section, not present in derCD23. It is therefore likely that only a negative-feedback mechanism operates with derCD23 to regulate IgE synthesis. Further investigation of the binding of CD23 fragments to SCR 5-8 of CD21 and the effect of this on IgE synthesis may lead to a potential therapeutic role for derCD23 in the treatment of allergic disease. Data accumulated in this study suggests that investigating the modulation of oligomeric state and thus the activity of soluble CD23 fragments may be important in the construction of new regulators of IgE synthesis.
- Full Text:
- Date Issued: 2010
Artificial neural networks as simulators for behavioural evolution in evolutionary robotics
- Pretorius, Christiaan Johannes
- Authors: Pretorius, Christiaan Johannes
- Date: 2010
- Subjects: Neural networks (Computer science) , Robotics
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10462 , http://hdl.handle.net/10948/1476 , Neural networks (Computer science) , Robotics
- Description: Robotic simulators for use in Evolutionary Robotics (ER) have certain challenges associated with the complexity of their construction and the accuracy of predictions made by these simulators. Such robotic simulators are often based on physics models, which have been shown to produce accurate results. However, the construction of physics-based simulators can be complex and time-consuming. Alternative simulation schemes construct robotic simulators from empirically-collected data. Such empirical simulators, however, also have associated challenges, such as that some of these simulators do not generalize well on the data from which they are constructed, as these models employ simple interpolation on said data. As a result of the identified challenges in existing robotic simulators for use in ER, this project investigates the potential use of Artificial Neural Networks, henceforth simply referred to as Neural Networks (NNs), as alternative robotic simulators. In contrast to physics models, NN-based simulators can be constructed without needing an explicit mathematical model of the system being modeled, which can simplify simulator development. Furthermore, the generalization capabilities of NNs suggest that NNs could generalize well on data from which these simulators are constructed. These generalization abilities of NNs, along with NNs’ noise tolerance, suggest that NNs could be well-suited to application in robotics simulation. Investigating whether NNs can be effectively used as robotic simulators in ER is thus the endeavour of this work. Since not much research has been done in employing NNs as robotic simulators, many aspects of the experimental framework on which this dissertation reports needed to be carefully decided upon. Two robot morphologies were selected on which the NN simulators created in this work were based, namely a differentially steered robot and an inverted pendulum robot. Motion tracking and robotic sensor logging were used to acquire data from which the NN simulators were constructed. Furthermore, custom code was written for almost all aspects of the study, namely data acquisition for NN training, the actual NN training process, the evolution of robotic controllers using the created NN simulators, as well as the onboard robotic implementations of evolved controllers. Experimental tests performed in order to determine ideal topologies for each of the NN simulators developed in this study indicated that different NN topologies can lead to large differences in training accuracy. After performing these tests, the training accuracy of the created simulators was analyzed. This analysis showed that the NN simulators generally trained well and could generalize well on data not presented during simulator construction. In order to validate the feasibility of the created NN simulators in the ER process, these simulators were subsequently used to evolve controllers in simulation, similar to controllers developed in related studies. Encouraging results were obtained, with the newly-evolved controllers allowing real-world experimental robots to exhibit obstacle avoidance and light-approaching behaviour with a reasonable degree of success. The created NN simulators furthermore allowed for the successful evolution of a complex inverted pendulum stabilization controller in simulation. It was thus clearly established that NN-based robotic simulators can be successfully employed as alternative simulation schemes in the ER process.
- Full Text:
- Date Issued: 2010
- Authors: Pretorius, Christiaan Johannes
- Date: 2010
- Subjects: Neural networks (Computer science) , Robotics
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10462 , http://hdl.handle.net/10948/1476 , Neural networks (Computer science) , Robotics
- Description: Robotic simulators for use in Evolutionary Robotics (ER) have certain challenges associated with the complexity of their construction and the accuracy of predictions made by these simulators. Such robotic simulators are often based on physics models, which have been shown to produce accurate results. However, the construction of physics-based simulators can be complex and time-consuming. Alternative simulation schemes construct robotic simulators from empirically-collected data. Such empirical simulators, however, also have associated challenges, such as that some of these simulators do not generalize well on the data from which they are constructed, as these models employ simple interpolation on said data. As a result of the identified challenges in existing robotic simulators for use in ER, this project investigates the potential use of Artificial Neural Networks, henceforth simply referred to as Neural Networks (NNs), as alternative robotic simulators. In contrast to physics models, NN-based simulators can be constructed without needing an explicit mathematical model of the system being modeled, which can simplify simulator development. Furthermore, the generalization capabilities of NNs suggest that NNs could generalize well on data from which these simulators are constructed. These generalization abilities of NNs, along with NNs’ noise tolerance, suggest that NNs could be well-suited to application in robotics simulation. Investigating whether NNs can be effectively used as robotic simulators in ER is thus the endeavour of this work. Since not much research has been done in employing NNs as robotic simulators, many aspects of the experimental framework on which this dissertation reports needed to be carefully decided upon. Two robot morphologies were selected on which the NN simulators created in this work were based, namely a differentially steered robot and an inverted pendulum robot. Motion tracking and robotic sensor logging were used to acquire data from which the NN simulators were constructed. Furthermore, custom code was written for almost all aspects of the study, namely data acquisition for NN training, the actual NN training process, the evolution of robotic controllers using the created NN simulators, as well as the onboard robotic implementations of evolved controllers. Experimental tests performed in order to determine ideal topologies for each of the NN simulators developed in this study indicated that different NN topologies can lead to large differences in training accuracy. After performing these tests, the training accuracy of the created simulators was analyzed. This analysis showed that the NN simulators generally trained well and could generalize well on data not presented during simulator construction. In order to validate the feasibility of the created NN simulators in the ER process, these simulators were subsequently used to evolve controllers in simulation, similar to controllers developed in related studies. Encouraging results were obtained, with the newly-evolved controllers allowing real-world experimental robots to exhibit obstacle avoidance and light-approaching behaviour with a reasonable degree of success. The created NN simulators furthermore allowed for the successful evolution of a complex inverted pendulum stabilization controller in simulation. It was thus clearly established that NN-based robotic simulators can be successfully employed as alternative simulation schemes in the ER process.
- Full Text:
- Date Issued: 2010
Assessing the diet of the Cape leopard (panthera pardus) in the Cederberg and Gamka mountains, South Africa
- Authors: Rautenbach, Therèsè
- Date: 2010
- Subjects: Leopard -- South Africa , Animal feeding -- South Africa , Predation (Biology)
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10699 , http://hdl.handle.net/10948/1078 , Leopard -- South Africa , Animal feeding -- South Africa , Predation (Biology)
- Description: As limited prey availability and persecution by humans in response to livestock predation are key conservation concerns for the Cape leopard (Martins & Martins 2006), the present study aimed to provide more information regarding their feeding habits. The first objective was to determine whether the Cape leopard was subject to a change in their prey base and how they responded to the change. This was established by comparison of their current diet in the Cederberg and Gamka Mountains, determined by using scat analysis techniques, with a previous dietary assessment (Norton et al. 1986). The second aim was to provide a preliminary assessment of the prey preference of the Cape leopard and examine the utility of camera trap surveys to determine leopard prey preference. Leopard diet in the Cederberg and Gamka Mountains consisted largely of small- (1-10 kg) and medium-sized (10-40 kg) mammals; rock hyraxes (Procavia capensis) and klipspringers (Oreotragus oreotragus) were key prey items. In terms of regional variation in leopard diet, there was a significant difference in the average weight of prey utilized in the Cederberg and Gamka Mountains. Despite the importance of prey availability of suitable size, their flexibility in terms of prey size utilization reflected their ability to switch to smaller prey to fulfil their dietary requirements, when prey is limited. The study suggested a dietary shift, with significant variation in prey species utilization in both regions. The shift did not appear to be in response to prey scarcity, but rather a reduction in key prey species, particularly the rock hyrax. The shift involved an increase in the number of species utilized, and only a very small increase in livestock predation in both areas. There was however no significant variation in prey size category utilization. This demonstrated their dietary flexibility, as well as the importance of suitable prey sizes rather than the presence of specific prey species to fulfil their dietary requirements. The camera trap survey revealed a strong correlation between the number of camera trap days and the number of photographs taken of identifiable species. Variation of this correlation between different habitats supported the notion that individual images are a better unit to determine sampling efficiency than trap days. The camera trap survey also showed that small rodent availability was underestimated by camera trap surveillance, which resulted in poor prey preference estimation. It was therefore suggested that camera trap surveys be restricted to the surveillance of larger prey species (> 1 kg). By excluding small rodents from the analysis, prey preference could be estimated for other species and prey size categories. Small- and medium-sized mammals were significantly preferred, whereas large mammals were significantly avoided by the Cape leopard.
- Full Text:
- Date Issued: 2010
- Authors: Rautenbach, Therèsè
- Date: 2010
- Subjects: Leopard -- South Africa , Animal feeding -- South Africa , Predation (Biology)
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10699 , http://hdl.handle.net/10948/1078 , Leopard -- South Africa , Animal feeding -- South Africa , Predation (Biology)
- Description: As limited prey availability and persecution by humans in response to livestock predation are key conservation concerns for the Cape leopard (Martins & Martins 2006), the present study aimed to provide more information regarding their feeding habits. The first objective was to determine whether the Cape leopard was subject to a change in their prey base and how they responded to the change. This was established by comparison of their current diet in the Cederberg and Gamka Mountains, determined by using scat analysis techniques, with a previous dietary assessment (Norton et al. 1986). The second aim was to provide a preliminary assessment of the prey preference of the Cape leopard and examine the utility of camera trap surveys to determine leopard prey preference. Leopard diet in the Cederberg and Gamka Mountains consisted largely of small- (1-10 kg) and medium-sized (10-40 kg) mammals; rock hyraxes (Procavia capensis) and klipspringers (Oreotragus oreotragus) were key prey items. In terms of regional variation in leopard diet, there was a significant difference in the average weight of prey utilized in the Cederberg and Gamka Mountains. Despite the importance of prey availability of suitable size, their flexibility in terms of prey size utilization reflected their ability to switch to smaller prey to fulfil their dietary requirements, when prey is limited. The study suggested a dietary shift, with significant variation in prey species utilization in both regions. The shift did not appear to be in response to prey scarcity, but rather a reduction in key prey species, particularly the rock hyrax. The shift involved an increase in the number of species utilized, and only a very small increase in livestock predation in both areas. There was however no significant variation in prey size category utilization. This demonstrated their dietary flexibility, as well as the importance of suitable prey sizes rather than the presence of specific prey species to fulfil their dietary requirements. The camera trap survey revealed a strong correlation between the number of camera trap days and the number of photographs taken of identifiable species. Variation of this correlation between different habitats supported the notion that individual images are a better unit to determine sampling efficiency than trap days. The camera trap survey also showed that small rodent availability was underestimated by camera trap surveillance, which resulted in poor prey preference estimation. It was therefore suggested that camera trap surveys be restricted to the surveillance of larger prey species (> 1 kg). By excluding small rodents from the analysis, prey preference could be estimated for other species and prey size categories. Small- and medium-sized mammals were significantly preferred, whereas large mammals were significantly avoided by the Cape leopard.
- Full Text:
- Date Issued: 2010
Attitudes towards hunting amongst the economically active public in Port Elizabeth
- Coetzer, Wentzel Christoffel
- Authors: Coetzer, Wentzel Christoffel
- Date: 2010
- Subjects: Hunting -- South Africa -- Port Elizabeth , Wildlife management -- South Africa -- Port Elizabeth
- Language: English
- Type: Thesis , Masters , MTech
- Identifier: vital:10746 , http://hdl.handle.net/10948/1507 , Hunting -- South Africa -- Port Elizabeth , Wildlife management -- South Africa -- Port Elizabeth
- Description: Wildlife ranching is recognized as one of the important agricultural industries in South Africa, with hunting being the primary segment of the game industry. Hunting is an essential part of wildlife management and it contributes significantly to the conservation of biodiversity and to the economy. Despite this, the social acceptability of hunting is often questioned and negative attitudes towards hunting within the larger society may become a potential threat to the hunting industry. This may jeopardize the effective management of game and have a negative impact on conservation and the economy. This study is an empirical investigation of the attitudes of the economically active public in Port Elizabeth towards hunting. The objectives of this study are to investigate the nature and extent of the public's attitudes towards hunting, as well as to identify factors influencing the public's attitudes towards hunting. The study commenced with the establishment of a theoretical framework, based on a literature study on the psychology of human perceptions and attitudes towards hunting. The theoretical framework was then used to develop the empirical component of the study. A survey was conducted amongst a statistically representative sample of the economically active public in Port Elizabeth during 2009. The data was collected by means of personal interviews and self-administered questionnaires were used as the survey instrument. The study found that the economically active public in Port Elizabeth generally holds favourable attitudes towards hunting, with only a small proportion of the population wanting to ban hunting. The belief that hunting endangers wildlife was the most prominent reason for opposing hunting. Negative attitudes towards hunting were largely based on incorrect beliefs regarding hunting. The extent of a person's knowledge of hunting and exposure to hunting, as well as a person's social ties had a significant influence on their attitudes towards hunting.
- Full Text:
- Date Issued: 2010
- Authors: Coetzer, Wentzel Christoffel
- Date: 2010
- Subjects: Hunting -- South Africa -- Port Elizabeth , Wildlife management -- South Africa -- Port Elizabeth
- Language: English
- Type: Thesis , Masters , MTech
- Identifier: vital:10746 , http://hdl.handle.net/10948/1507 , Hunting -- South Africa -- Port Elizabeth , Wildlife management -- South Africa -- Port Elizabeth
- Description: Wildlife ranching is recognized as one of the important agricultural industries in South Africa, with hunting being the primary segment of the game industry. Hunting is an essential part of wildlife management and it contributes significantly to the conservation of biodiversity and to the economy. Despite this, the social acceptability of hunting is often questioned and negative attitudes towards hunting within the larger society may become a potential threat to the hunting industry. This may jeopardize the effective management of game and have a negative impact on conservation and the economy. This study is an empirical investigation of the attitudes of the economically active public in Port Elizabeth towards hunting. The objectives of this study are to investigate the nature and extent of the public's attitudes towards hunting, as well as to identify factors influencing the public's attitudes towards hunting. The study commenced with the establishment of a theoretical framework, based on a literature study on the psychology of human perceptions and attitudes towards hunting. The theoretical framework was then used to develop the empirical component of the study. A survey was conducted amongst a statistically representative sample of the economically active public in Port Elizabeth during 2009. The data was collected by means of personal interviews and self-administered questionnaires were used as the survey instrument. The study found that the economically active public in Port Elizabeth generally holds favourable attitudes towards hunting, with only a small proportion of the population wanting to ban hunting. The belief that hunting endangers wildlife was the most prominent reason for opposing hunting. Negative attitudes towards hunting were largely based on incorrect beliefs regarding hunting. The extent of a person's knowledge of hunting and exposure to hunting, as well as a person's social ties had a significant influence on their attitudes towards hunting.
- Full Text:
- Date Issued: 2010
Beta-N-methylamino-L-alanine in South African fresh water cyanobacteria : incidence, prevalence, ecotoxicological considerations and human exposure risk
- Authors: Esterhuizen-Londt, Maranda
- Date: 2010
- Subjects: Cyanobacteria , Bioaccumulation , Chromatographic analysis , Neurotoxic agents
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:10306 , http://hdl.handle.net/10948/1473 , Cyanobacteria , Bioaccumulation , Chromatographic analysis , Neurotoxic agents
- Description: β-N-methylamino-L-alanine (BMAA) is a non-proteinogenic amino acid associated with human neurodegenerative disease. Due to the cosmopolitan nature of cyanobacteria, detection of BMAA in cyanobacteria has caused concerns about human exposure risk. This study was therefore based on the hypothesis that BMAA poses a health risk to humans either by direct ingestion or by indirect exposure to BMAA from a cyanobacterial source via a freshwater food chain. A validated gas chromatography-mass spectrometry (GC-MS) BMAA analysis method and a confirmatory liquid chromatography-mass spectrometry (LC-MS) method, with improved sensitivity, were developed in addition to a LC-MS/MS method for analyte confirmation. These methods were used to quantify BMAA in South African cyanobacteria, isolated from various potable water reservoirs. The majority of the isolates tested, contained BMAA. Possible human exposure by direct consumption of BMAA released from cyanobacterial blooms was investigated by the development of a robust solid phase extraction (SPE) method used for BMAA concentration and quantification in raw and treated tap water. Despite the use of the SPE method that facilitated the concentration of BMAA from large quantities of water, no free dissolved BMAA was detected in raw or processed fresh water. The fate of exogenous BMAA was therefore investigated firstly by evaluating the efficacy of standard water treatment processes employed in South Africa and secondly by investigating the possibility of BMAA bioaccumulation and biomagnification in aquatic food chains. Standard water treatment processes proved highly efficient at removing free dissolved BMAA, explaining the absence of BMAA in treated tap water. However, the cause of the BMAA absence in raw potable water remained unknown. Uptake of BMAA by model aquatic organisms was investigated in controlled experiments. BMAA uptake was documented in both Ceratophyllum demersum and Daphnia magna, however, BMAA-protein association and biomagnification were not observed in D. magna. BMAA had an inhibitory effect on the oxidative stress enzyme acitivties of both organisms tested (as well as human S9 extracts), resulting in accumulation of detrimental reactive oxygen species (ROS) in the cells. Exposure of crop plants to BMAA in controlled experiments resulted in BMAA uptake, protein association, and subsequent inhibition of the antioxidative enzyme activities. However, BMAA was detected in neither free nor protein-associated form in natural crop plants irrigated with known BMAA-containing bloom water. Post-mortem liver samples of Clarias gariepinus (Catfish) and Crocodylus niloticus (Crocodile), from a natural fresh water ecosystem that experienced frequent cyanobacterial blooms, contained both free and protein-associated BMAA. Higher BMAA concentrations were found in crocodile liver samples compared to fish liver samples, strongly suggesting biomagnification from one trophic level to the next. BMAA concentrations corresponded to crocodile age. This is the first report of bioaccumulation and biomagnification in two trophic levels in a fresh water ecosystem. These findings strongly suggest possible human exposure via aquatic food chains of cyanobacterial origin. Direct BMAA exposure via drinking water is not plausible due to the efficiency of standard water treatment processes to remove BMAA. The use of raw water for agricultural and recreational use, however, remains a problem. The development of management strategies as well as daily tolerable levels for BMAA is urgently required.
- Full Text:
- Date Issued: 2010
- Authors: Esterhuizen-Londt, Maranda
- Date: 2010
- Subjects: Cyanobacteria , Bioaccumulation , Chromatographic analysis , Neurotoxic agents
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:10306 , http://hdl.handle.net/10948/1473 , Cyanobacteria , Bioaccumulation , Chromatographic analysis , Neurotoxic agents
- Description: β-N-methylamino-L-alanine (BMAA) is a non-proteinogenic amino acid associated with human neurodegenerative disease. Due to the cosmopolitan nature of cyanobacteria, detection of BMAA in cyanobacteria has caused concerns about human exposure risk. This study was therefore based on the hypothesis that BMAA poses a health risk to humans either by direct ingestion or by indirect exposure to BMAA from a cyanobacterial source via a freshwater food chain. A validated gas chromatography-mass spectrometry (GC-MS) BMAA analysis method and a confirmatory liquid chromatography-mass spectrometry (LC-MS) method, with improved sensitivity, were developed in addition to a LC-MS/MS method for analyte confirmation. These methods were used to quantify BMAA in South African cyanobacteria, isolated from various potable water reservoirs. The majority of the isolates tested, contained BMAA. Possible human exposure by direct consumption of BMAA released from cyanobacterial blooms was investigated by the development of a robust solid phase extraction (SPE) method used for BMAA concentration and quantification in raw and treated tap water. Despite the use of the SPE method that facilitated the concentration of BMAA from large quantities of water, no free dissolved BMAA was detected in raw or processed fresh water. The fate of exogenous BMAA was therefore investigated firstly by evaluating the efficacy of standard water treatment processes employed in South Africa and secondly by investigating the possibility of BMAA bioaccumulation and biomagnification in aquatic food chains. Standard water treatment processes proved highly efficient at removing free dissolved BMAA, explaining the absence of BMAA in treated tap water. However, the cause of the BMAA absence in raw potable water remained unknown. Uptake of BMAA by model aquatic organisms was investigated in controlled experiments. BMAA uptake was documented in both Ceratophyllum demersum and Daphnia magna, however, BMAA-protein association and biomagnification were not observed in D. magna. BMAA had an inhibitory effect on the oxidative stress enzyme acitivties of both organisms tested (as well as human S9 extracts), resulting in accumulation of detrimental reactive oxygen species (ROS) in the cells. Exposure of crop plants to BMAA in controlled experiments resulted in BMAA uptake, protein association, and subsequent inhibition of the antioxidative enzyme activities. However, BMAA was detected in neither free nor protein-associated form in natural crop plants irrigated with known BMAA-containing bloom water. Post-mortem liver samples of Clarias gariepinus (Catfish) and Crocodylus niloticus (Crocodile), from a natural fresh water ecosystem that experienced frequent cyanobacterial blooms, contained both free and protein-associated BMAA. Higher BMAA concentrations were found in crocodile liver samples compared to fish liver samples, strongly suggesting biomagnification from one trophic level to the next. BMAA concentrations corresponded to crocodile age. This is the first report of bioaccumulation and biomagnification in two trophic levels in a fresh water ecosystem. These findings strongly suggest possible human exposure via aquatic food chains of cyanobacterial origin. Direct BMAA exposure via drinking water is not plausible due to the efficiency of standard water treatment processes to remove BMAA. The use of raw water for agricultural and recreational use, however, remains a problem. The development of management strategies as well as daily tolerable levels for BMAA is urgently required.
- Full Text:
- Date Issued: 2010
Bioaccumulation and ecotoxicology of b-methylamino-l-alanine (BMAA) in model crop plants
- Niyonzima, Francois Niyongabo
- Authors: Niyonzima, Francois Niyongabo
- Date: 2010
- Subjects: Cyanobacteria , Environmental toxicology
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10312 , http://hdl.handle.net/10948/1475 , Cyanobacteria , Environmental toxicology
- Description: Cyanobacteria are known to produce a variety of toxic compounds. β-N-methylamino-L-alanine (BMAA) is one of the neurotoxins produced by most cyanobacteria. BMAA has been implicated in amyotrophic lateral sclerosis / Parkinsonism dementia complex (ALS / PDC) and was suggested to contribute to this pathology after biomagnification and slow release of BMAA from a protein associated form. The uptake and accumulation of BMAA by the aquatic macrophyte Ceratophyllum demersum has recently been shown, but the consumption of aquatic macrophytes by humans is not typical. The uptake by, and accumulation in, crop plants (Nasturtium officinale and Daucus carota) was therefore investigated so as to establish the existence of any risk to humans from the consumption of plants irrigated with water from dams with high cyanobacterial biomass and therefore high BMAA levels. After the exposure to the BMAA through the growth medium, BMAA had no effect on growth and development of N. officinale and D. carota. The uptake and bioaccumulation of BMAA was observed in N. officinale and D. carota, and was found to be concentration-dependent. Both free and bound cellular BMAA was detected following BMAA exposure through the growth medium. The photosynthetic apparatus of N. officinale was not significantly damaged. The uptake and accumulation of BMAA in edible terrestrial plants may constitute another route of human exposure to BMAA; it may now be prudent to avoid spray irrigation of edible plants with waters from dams with high cyanobacterial biomass and therefore high BMAA levels. After uptake by plants, the cyanotoxins may induce oxidative stress. A recent study showed that BMAA has a significant inhibitory effect on the oxidative stress enzymes in C. demersum. Therefore, the toxicological effects on selected plants were investigated by a range of biochemical enzyme assays in order to establish the plant stress response to exogenous BMAA. The inhibition of antioxidant enzymes upon exposure of N. officinale to BMAA through the growth medium was observed. The inhibition of antioxidant defence enzymes by BMAA correlated with the BMAA bioaccumulation in N. officinale. Further investigations are needed to analyze the uptake, accumulation, and ecotoxicology of BMAA in other crop plants, and to examine the fate of BMAA in these plants particularly its distribution and metabolism.
- Full Text:
- Date Issued: 2010
- Authors: Niyonzima, Francois Niyongabo
- Date: 2010
- Subjects: Cyanobacteria , Environmental toxicology
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10312 , http://hdl.handle.net/10948/1475 , Cyanobacteria , Environmental toxicology
- Description: Cyanobacteria are known to produce a variety of toxic compounds. β-N-methylamino-L-alanine (BMAA) is one of the neurotoxins produced by most cyanobacteria. BMAA has been implicated in amyotrophic lateral sclerosis / Parkinsonism dementia complex (ALS / PDC) and was suggested to contribute to this pathology after biomagnification and slow release of BMAA from a protein associated form. The uptake and accumulation of BMAA by the aquatic macrophyte Ceratophyllum demersum has recently been shown, but the consumption of aquatic macrophytes by humans is not typical. The uptake by, and accumulation in, crop plants (Nasturtium officinale and Daucus carota) was therefore investigated so as to establish the existence of any risk to humans from the consumption of plants irrigated with water from dams with high cyanobacterial biomass and therefore high BMAA levels. After the exposure to the BMAA through the growth medium, BMAA had no effect on growth and development of N. officinale and D. carota. The uptake and bioaccumulation of BMAA was observed in N. officinale and D. carota, and was found to be concentration-dependent. Both free and bound cellular BMAA was detected following BMAA exposure through the growth medium. The photosynthetic apparatus of N. officinale was not significantly damaged. The uptake and accumulation of BMAA in edible terrestrial plants may constitute another route of human exposure to BMAA; it may now be prudent to avoid spray irrigation of edible plants with waters from dams with high cyanobacterial biomass and therefore high BMAA levels. After uptake by plants, the cyanotoxins may induce oxidative stress. A recent study showed that BMAA has a significant inhibitory effect on the oxidative stress enzymes in C. demersum. Therefore, the toxicological effects on selected plants were investigated by a range of biochemical enzyme assays in order to establish the plant stress response to exogenous BMAA. The inhibition of antioxidant enzymes upon exposure of N. officinale to BMAA through the growth medium was observed. The inhibition of antioxidant defence enzymes by BMAA correlated with the BMAA bioaccumulation in N. officinale. Further investigations are needed to analyze the uptake, accumulation, and ecotoxicology of BMAA in other crop plants, and to examine the fate of BMAA in these plants particularly its distribution and metabolism.
- Full Text:
- Date Issued: 2010
Customer profiling using a service-orientated architecture
- Authors: Ntawanga, Felix
- Date: 2010
- Subjects: Consumer profiling -- South Africa , Consumer behavior -- South Africa , Service-oriented architecture (Computer science) , Electronic commerce -- South Africa -- Computer programs
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10464 , http://hdl.handle.net/10948/1146 , Consumer profiling -- South Africa , Consumer behavior -- South Africa , Service-oriented architecture (Computer science) , Electronic commerce -- South Africa -- Computer programs
- Description: Customer profiling has recently gained much recognition in the e-commerce domain because of the benefits it is capable of bringing to online business. Customer profiling has been implemented in various systems development approaches such as in a client-server environment. Recently there has been an increase in the number of organisations adopting and implementing e-commerce systems using service-oriented architecture (SOA) principles. This research set out to determine how a customer profile can be implemented using open source SOA implementation tools, and how SOA-based customer profiles can be utilised to provide appropriate personalisation in an SOA environment. The research further endeavoured to complete a comparative study on customer profile implementation in two different architectures, namely SOA and client-server. An extensive literature review was conducted on SOA, customer profiling and e-commerce systems development. SOA enabling technologies, such as, web services, enterprise service bus (ESB) and open source Sun Java SOA implementation tools, for example, Open ESB, GlassFish application server and Netbeans IDE were analysed. A Java web services-based customer profiling system was prototyped following SOA design principles. An end-user evaluation survey was conducted using eye tracking with a sample of 30 participants. The evaluation was done on two e-commerce systems with the same interface but running on two different customer profile back-ends, SOA and client-server. The results show that participants did not experience significant difference between the two systems, however, eye tracking results showed a significant difference between the two systems. The research concluded that customer profiling using SOA offers more benefits than implementations using other architectures such as client-server. SOA component-based development proved to be easier to manage, develop, integrate and improves interoperability between different technologies. The research brought together necessary techniques and technologies that organisations can use to implement SOA. Using SOA, organisations can integrate and utilise different technologies seamlessly to achieve business goals.
- Full Text:
- Date Issued: 2010
- Authors: Ntawanga, Felix
- Date: 2010
- Subjects: Consumer profiling -- South Africa , Consumer behavior -- South Africa , Service-oriented architecture (Computer science) , Electronic commerce -- South Africa -- Computer programs
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10464 , http://hdl.handle.net/10948/1146 , Consumer profiling -- South Africa , Consumer behavior -- South Africa , Service-oriented architecture (Computer science) , Electronic commerce -- South Africa -- Computer programs
- Description: Customer profiling has recently gained much recognition in the e-commerce domain because of the benefits it is capable of bringing to online business. Customer profiling has been implemented in various systems development approaches such as in a client-server environment. Recently there has been an increase in the number of organisations adopting and implementing e-commerce systems using service-oriented architecture (SOA) principles. This research set out to determine how a customer profile can be implemented using open source SOA implementation tools, and how SOA-based customer profiles can be utilised to provide appropriate personalisation in an SOA environment. The research further endeavoured to complete a comparative study on customer profile implementation in two different architectures, namely SOA and client-server. An extensive literature review was conducted on SOA, customer profiling and e-commerce systems development. SOA enabling technologies, such as, web services, enterprise service bus (ESB) and open source Sun Java SOA implementation tools, for example, Open ESB, GlassFish application server and Netbeans IDE were analysed. A Java web services-based customer profiling system was prototyped following SOA design principles. An end-user evaluation survey was conducted using eye tracking with a sample of 30 participants. The evaluation was done on two e-commerce systems with the same interface but running on two different customer profile back-ends, SOA and client-server. The results show that participants did not experience significant difference between the two systems, however, eye tracking results showed a significant difference between the two systems. The research concluded that customer profiling using SOA offers more benefits than implementations using other architectures such as client-server. SOA component-based development proved to be easier to manage, develop, integrate and improves interoperability between different technologies. The research brought together necessary techniques and technologies that organisations can use to implement SOA. Using SOA, organisations can integrate and utilise different technologies seamlessly to achieve business goals.
- Full Text:
- Date Issued: 2010
Development of insulin resistance in a rat model and the effects of sutherlandia frutescens as treatment and prevention
- Authors: Mackenzie, Janine
- Date: 2010
- Subjects: Metabolic syndrome , Insulin resistance -- Animal models , Obesity -- Treatment
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: vital:10340 , http://hdl.handle.net/10948/d1016216
- Description: The global number of obese people has reached pandemic proportions. High caloric diets and reduced physical exercise are to blame for this growing epidemic. Obesity has a very complex association with several other metabolic disorders, such as insulin resistance (IR), diabetes mellitus type 2 (DMT2) and cardiovascular disease. This puts a huge burden on health care systems world wide and claims many lives. Sutherlandia frutescens is a traditionally used herb, which is known to have anti-diabetic properties. However, the direct mode of action of S. frutescens still remains to be elucidated. The aim of this study was to investigate the developmental stages of high fat diet (HFD)-induced IR, to illuminate the pathogenesis of IR with a focal point on modifications in the lipid metabolism. Furthermore, the effects of S. frutescens as a treatment or prevention drug for IR and associated metabolic changes were examined. Two sets of experiments were conducted on male Wistar rats. In the first experiment rats, one week post weaning received a low fat diet (LFD), high fat diet (HFD) or HFD supplemented with S. frutescens (50mg/kg BW/d). Rats were sacrificed at week 0, 1, 2, 4, 8 and 12 in the feeding regime. In a second experiment rats were fed with a LFD or a HFD for 12 weeks and treated thereafter with S. frutescens (50mg/kg BW/d), metformin (13mg/kg BW/d) or water (control) for 28 days. Rats in the second experiment were sacrificed at week 12 to confirm IR while concurrently run rats were sacrificed after 28 days of treatment. For all the experiments rats were anaesthetized, blood was removed and rats were dissected. Plasma samples were analyzed for insulin, glucose, blood lipid parameters and cytokines. Liver, muscle and adipose tissue were analyzed for glucose uptake, total lipid content, lipid profile and fatty acid profile. It was shown that the intake of HFD caused IR and hyperinsulinaemia. The developmental stages in experiment one confirmed that an increase in plasma free fatty acids preceeded the onset of IR. Plasma and tissue lipid parameters (free fatty acid-, triglyceride- and cholesterol concentrations) showed pathological modifications in the HFD group. An ectopic accumulation of fat was observed in muscle and liver, as well as a change in membrane fatty acid profile. The results for circulating cytokines were somewhat inconclusive. Rats supplemented with S. frutescens did not develop HFD-induced IR (study one) or IR was reversed (study two). S. frutescens treatment also resulted in positive changes in plasma and tissue lipid parameters. In summary, an animal model for HFD-induced IR was established and the detrimental effect of elevated plasma FFA on glucose and lipid metabolism was observed. A novel discovery suggests that the anti-diabetic mode of action of S. frutescens is through modulation of lipid metabolism. It was also established that S. frutescens has the potential to prevent IR in vivo.
- Full Text:
- Date Issued: 2010
- Authors: Mackenzie, Janine
- Date: 2010
- Subjects: Metabolic syndrome , Insulin resistance -- Animal models , Obesity -- Treatment
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: vital:10340 , http://hdl.handle.net/10948/d1016216
- Description: The global number of obese people has reached pandemic proportions. High caloric diets and reduced physical exercise are to blame for this growing epidemic. Obesity has a very complex association with several other metabolic disorders, such as insulin resistance (IR), diabetes mellitus type 2 (DMT2) and cardiovascular disease. This puts a huge burden on health care systems world wide and claims many lives. Sutherlandia frutescens is a traditionally used herb, which is known to have anti-diabetic properties. However, the direct mode of action of S. frutescens still remains to be elucidated. The aim of this study was to investigate the developmental stages of high fat diet (HFD)-induced IR, to illuminate the pathogenesis of IR with a focal point on modifications in the lipid metabolism. Furthermore, the effects of S. frutescens as a treatment or prevention drug for IR and associated metabolic changes were examined. Two sets of experiments were conducted on male Wistar rats. In the first experiment rats, one week post weaning received a low fat diet (LFD), high fat diet (HFD) or HFD supplemented with S. frutescens (50mg/kg BW/d). Rats were sacrificed at week 0, 1, 2, 4, 8 and 12 in the feeding regime. In a second experiment rats were fed with a LFD or a HFD for 12 weeks and treated thereafter with S. frutescens (50mg/kg BW/d), metformin (13mg/kg BW/d) or water (control) for 28 days. Rats in the second experiment were sacrificed at week 12 to confirm IR while concurrently run rats were sacrificed after 28 days of treatment. For all the experiments rats were anaesthetized, blood was removed and rats were dissected. Plasma samples were analyzed for insulin, glucose, blood lipid parameters and cytokines. Liver, muscle and adipose tissue were analyzed for glucose uptake, total lipid content, lipid profile and fatty acid profile. It was shown that the intake of HFD caused IR and hyperinsulinaemia. The developmental stages in experiment one confirmed that an increase in plasma free fatty acids preceeded the onset of IR. Plasma and tissue lipid parameters (free fatty acid-, triglyceride- and cholesterol concentrations) showed pathological modifications in the HFD group. An ectopic accumulation of fat was observed in muscle and liver, as well as a change in membrane fatty acid profile. The results for circulating cytokines were somewhat inconclusive. Rats supplemented with S. frutescens did not develop HFD-induced IR (study one) or IR was reversed (study two). S. frutescens treatment also resulted in positive changes in plasma and tissue lipid parameters. In summary, an animal model for HFD-induced IR was established and the detrimental effect of elevated plasma FFA on glucose and lipid metabolism was observed. A novel discovery suggests that the anti-diabetic mode of action of S. frutescens is through modulation of lipid metabolism. It was also established that S. frutescens has the potential to prevent IR in vivo.
- Full Text:
- Date Issued: 2010
Diet and population trends of warthog in the Addo Elephant National Park
- Authors: Mgqatsa, Nokubonga
- Date: 2010
- Subjects: Warthog -- South Africa -- Addo Elelphant National Park
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10693 , http://hdl.handle.net/10948/1277 , Warthog -- South Africa -- Addo Elelphant National Park
- Description: The common warthog (Phacochoerus africanus) has recently been recognised as an alien invasive species in the Eastern Cape and their population is increasing rapidly within the region. This then raises a concern as it is recorded that invasive species have negative impacts on both economic and ecological aspects of biodiversity in the receiving community. There are few studies that have documented the impacts of this species in the Eastern Cape. This study therefore seeks to determine the diet and population trends of this species in the Addo Elephant National Park (AENP) Main Camp and the results obtained can be correlated with previous studies and used to quantify the impacts of this species in the Eastern Cape. Warthogs are specialized grazers, it was therefore hypothesized that they will have potential impacts on grazing resources in the AENP Main Camp. The diet of common warthogs in the AENP Main Camp was determined through microhistological analysis of faecal material of samples collected seasonally. Population trends of common warthogs were determined in relation to the effects of predation on the population growth and population age structure of these animals within the AENP Main Camp. The results were compared with the findings for common warthog population growth and population age structure of Great Fish River Nature Reserve (GFRNR), a predator free population. It was hypothesized that the population growth rate of common warthogs in the AENP Main Camp before the introduction of lions and hyaenas in the AENP would not differ from the population growth rate of common warthogs in GFRNR. Secondly, the post-lion/hyaena common warthog population in the AENP Main Camp would differ from both the AENP Main Camp pre-lion/hyaena and GFRNR common warthog populations due to the presence of large predators in the AENP Main Camp. For population age structure it was hypothesized that the two populations, AENP Main Camp and GFRNR would differ because of predators increasing mortality. The diet of common warthogs was dominated by grass (87.4 percent), with Cynodon dactylon being the dominant grass species. Common warthogs in the AENP Main Camp should be properly managed because they have potential impact on grasses. The population growth of common warthogs in the AENP Main Camp showed no effect of predation, with population growth not differing from that of GFRNR. Predation had an effect on common warthog population age structure with AENP Main Camp and GFRNR populations differing, particularly in terms of adult structure. Thus, population growth of common warthogs in the AENP Main Camp is not determined by the presence of predators. These findings highlight the status of common warthog as an invasive species in the AENP Main Camp and potentially impacting on grass species and show little effects of top-down population regulation. These results show a need of monitoring common warthog population as well as their potential impacts in the area.
- Full Text:
- Date Issued: 2010
- Authors: Mgqatsa, Nokubonga
- Date: 2010
- Subjects: Warthog -- South Africa -- Addo Elelphant National Park
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10693 , http://hdl.handle.net/10948/1277 , Warthog -- South Africa -- Addo Elelphant National Park
- Description: The common warthog (Phacochoerus africanus) has recently been recognised as an alien invasive species in the Eastern Cape and their population is increasing rapidly within the region. This then raises a concern as it is recorded that invasive species have negative impacts on both economic and ecological aspects of biodiversity in the receiving community. There are few studies that have documented the impacts of this species in the Eastern Cape. This study therefore seeks to determine the diet and population trends of this species in the Addo Elephant National Park (AENP) Main Camp and the results obtained can be correlated with previous studies and used to quantify the impacts of this species in the Eastern Cape. Warthogs are specialized grazers, it was therefore hypothesized that they will have potential impacts on grazing resources in the AENP Main Camp. The diet of common warthogs in the AENP Main Camp was determined through microhistological analysis of faecal material of samples collected seasonally. Population trends of common warthogs were determined in relation to the effects of predation on the population growth and population age structure of these animals within the AENP Main Camp. The results were compared with the findings for common warthog population growth and population age structure of Great Fish River Nature Reserve (GFRNR), a predator free population. It was hypothesized that the population growth rate of common warthogs in the AENP Main Camp before the introduction of lions and hyaenas in the AENP would not differ from the population growth rate of common warthogs in GFRNR. Secondly, the post-lion/hyaena common warthog population in the AENP Main Camp would differ from both the AENP Main Camp pre-lion/hyaena and GFRNR common warthog populations due to the presence of large predators in the AENP Main Camp. For population age structure it was hypothesized that the two populations, AENP Main Camp and GFRNR would differ because of predators increasing mortality. The diet of common warthogs was dominated by grass (87.4 percent), with Cynodon dactylon being the dominant grass species. Common warthogs in the AENP Main Camp should be properly managed because they have potential impact on grasses. The population growth of common warthogs in the AENP Main Camp showed no effect of predation, with population growth not differing from that of GFRNR. Predation had an effect on common warthog population age structure with AENP Main Camp and GFRNR populations differing, particularly in terms of adult structure. Thus, population growth of common warthogs in the AENP Main Camp is not determined by the presence of predators. These findings highlight the status of common warthog as an invasive species in the AENP Main Camp and potentially impacting on grass species and show little effects of top-down population regulation. These results show a need of monitoring common warthog population as well as their potential impacts in the area.
- Full Text:
- Date Issued: 2010
Effects of salinity on the growth and lipid production of ten species of microalgae from the Swartkops saltworks : a biodiesel perspective
- Authors: Sonnekus, Martinus Jakobus
- Date: 2010
- Subjects: Microalgae -- South Africa -- Swartkops , Salinity -- South Africa -- Swartkops , Biodiesel fuels
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10618 , http://hdl.handle.net/10948/1097 , Microalgae -- South Africa -- Swartkops , Salinity -- South Africa -- Swartkops , Biodiesel fuels
- Description: Biodiesel from microalgae is a viable alternative for replacing the global demand for petro-diesel. High biomass and lipid production are key desirable characteristics needed in a species to be used for biodiesel production. It has been demonstrated in literature that the increase in salinity can increase the lipid content of microalgae, but lower the growth rate of a species. Therefore the effect that salinity has on the growth and lipid content of ten microalgal species, isolated from a warm temperate solar saltworks, was investigated. The microalgae were cultivated at a temperature of 22°C and at salinities ranging from 17 to 70 psu. It was found that growth and lipid production for all species were influenced to some degree by the salinity. Growth rates greater than 0.6 d-1 showed a decrease with higher salinity. Most (71 percent) of the growth rates that exceeded 0.6 per day were exhibited by cultures exposed to normal salinity (35 psu). This shift is a good indication that salinity inhibits/slows down growth and that the species in general prefer lower salinity conditions. Growth rates ranged from 0.17 ± 0.05 to 1.19 ± 0.17 d-1. Lipid content for the diatoms (2.78 ± 0.36 to 10.86 ± 4.59 percent DW) were lower than expected, whereas the lipid content for the green flagellates (3.10 ± 1.56 to 22.64 ± 1.19 percent DW) was on par with that reported in literature. To bring results into perspective a production model was developed to simulate a production scenario at the Swartkops Saltworks. Lipid and productivity results obtained in this study were used to estimate how much oil and biomass can be produced within the ponds of the Swartkops Saltworks. The model showed that although microalgae cultivation for biodiesel is technically feasible, at present it is not economically viable to do so.
- Full Text:
- Date Issued: 2010
- Authors: Sonnekus, Martinus Jakobus
- Date: 2010
- Subjects: Microalgae -- South Africa -- Swartkops , Salinity -- South Africa -- Swartkops , Biodiesel fuels
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10618 , http://hdl.handle.net/10948/1097 , Microalgae -- South Africa -- Swartkops , Salinity -- South Africa -- Swartkops , Biodiesel fuels
- Description: Biodiesel from microalgae is a viable alternative for replacing the global demand for petro-diesel. High biomass and lipid production are key desirable characteristics needed in a species to be used for biodiesel production. It has been demonstrated in literature that the increase in salinity can increase the lipid content of microalgae, but lower the growth rate of a species. Therefore the effect that salinity has on the growth and lipid content of ten microalgal species, isolated from a warm temperate solar saltworks, was investigated. The microalgae were cultivated at a temperature of 22°C and at salinities ranging from 17 to 70 psu. It was found that growth and lipid production for all species were influenced to some degree by the salinity. Growth rates greater than 0.6 d-1 showed a decrease with higher salinity. Most (71 percent) of the growth rates that exceeded 0.6 per day were exhibited by cultures exposed to normal salinity (35 psu). This shift is a good indication that salinity inhibits/slows down growth and that the species in general prefer lower salinity conditions. Growth rates ranged from 0.17 ± 0.05 to 1.19 ± 0.17 d-1. Lipid content for the diatoms (2.78 ± 0.36 to 10.86 ± 4.59 percent DW) were lower than expected, whereas the lipid content for the green flagellates (3.10 ± 1.56 to 22.64 ± 1.19 percent DW) was on par with that reported in literature. To bring results into perspective a production model was developed to simulate a production scenario at the Swartkops Saltworks. Lipid and productivity results obtained in this study were used to estimate how much oil and biomass can be produced within the ponds of the Swartkops Saltworks. The model showed that although microalgae cultivation for biodiesel is technically feasible, at present it is not economically viable to do so.
- Full Text:
- Date Issued: 2010
Implementation of novel flow cytometric methods to assess the in vitro antidiabetic mechanism of a Sutherlandia Frutescens extract
- Authors: Elliot, Gayle Pamela
- Date: 2010
- Subjects: Insulin resistance -- South Africa , Insulin -- Therapeutic use -- South Africa , Medicinal plants -- South Africa , Non-insulin-dependent diabetes -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:10304 , http://hdl.handle.net/10948/1439 , Insulin resistance -- South Africa , Insulin -- Therapeutic use -- South Africa , Medicinal plants -- South Africa , Non-insulin-dependent diabetes -- South Africa
- Description: The ability of insulin to stimulate glucose uptake into muscle and adipose tissue is central to the maintenance of whole-body glucose homeostasis. Deregulation of insulin action manifests itself as insulin resistance, a key component of type 2 diabetes. Insulin resistance is also observed in HIV patients receiving protease inhibitors. An agent that can reversibly induce an insulin-resistant state would be a very useful tool in developing model systems that mimic the pathogenesis of type 2 diabetes. Insulin resistance can arise from defects in insulin signal transduction, changes in the expression of proteins or genes that are targets of insulin action, cross talk from other hormonal systems or metabolic abnormalities. Deterioration of the insulin-receptor-signalling pathway at different levels leading to decreased levels of signalling pathway intermediates and/or decreased activation through phosphorylation accounts for the evolution from an insulin-resistant state to type 2 diabetes. In addition, defects in GLUT4 glucose transporter translocation are observed, further fuelling impairments in skeletal muscle glucose uptake. Levels of insulin-induced GLUT4 translocation in the skeletal muscle of type 2 diabetic patients are typically reduced by 90%. Many cellular pathways & their intermediates are in some way or another linked to insulin signalling. This study focused on three of these namely the PI3-kinase/Akt pathway, the Mitogen Activated Protein Kinase (MAPK) cascade and the AMP Kinase pathway, with successful monitoring of the PI3-K pathway. Investigations involved observing and evaluating the effects of various compounds as well as an indigenous medicinal plant, Sutherlandia frutescens on the activities of key insulin signalling pathway intermediates within the three fore mentioned pathways including Akt, AMPK and MEK1/2 as well as membrane surface GLUT4 levels. Scientific research has in the past leant heavily on Western blotting as the method of choice for gaining vital information relating to signal transduction pathways, however for research into cellular mechanisms the negatives of this method outweigh the positives. The drawbacks include a need for large amount of cells, multiple washing steps which may be disadvantageous to any weak and transient interactions as well as lysing of cells which may interfere with the maintenance of the subcellular localisation of a specific signalling event. Based on these, the need for a better method in terms of speed & reliability to monitor phosphorylation states of signal transduction pathway intermediates & GLUT4 translocation was evident and was one VII of the main aims & successes of this study. The method created used the mouse muscle cell line C2C12 in conjunction with the quick, sensitive method of flow cytometry which allowed us to monitor these processes in these cells through immune-labelling. Adherent cell cultures such as the C2C12 cell line pose the problem of possible damage to plasma membrane receptors (including insulin receptors) during harvesting to obtain a cell suspension for flow cytometry. We however used C2C12 mouse myocytes to optimize a method yielding insulin responsive cells in suspension that were successfully used for flow cytometry after immunelabelling of insulin signalling intermediates. Insulin (0.1μM) significantly raised the levels of both P-Akt and GLUT4 above basal levels. This effect was shown to be dose dependent. At a concentration of 50μg/ml, Sutherlandia frutescens was able to act as an insulin-mimetic in terms of its ability to increase P-Akt levels, GLUT4 translocation and glucose utilisation in an acute manner. These increases could be reduced with the addition of wortmannin, a PI3-K inhibitor. Therefore, these results suggest the mechanism of the plant extract’s insulin-like activity may be in part due to the activation of the insulin signalling pathway leading to GLUT4 translocation, which involves the phosphorylation of insulin receptor- and subsequent PI3-K activity, leading to P-Akt activity. These results provide further evidence of this plant extract’s anti-diabetic potential. The effect of Sutherlandia frutescens on insulin secretion, calcium signalling and proliferation in INS-1 rat pancreatic cells was also investigated and it was found to increase the activities of all of these processes. However no change in the levels of GLUT2 glucose transporter was seen. Ritonavir is prescribed by the South African Department of Health in co-formulation with other protease inhibitors within its second regime in the treatment of HIV and AIDS. Using C2C12 cells, ritonavir decreased glucose uptake acutely and had no effect on GLUT4 translocation however surprisingly increased P-Akt levels. In conclusion, it was found that Sutherlandia frutescens has antidiabetic benefits, diverse in nature depending on tissue type as well as length of time administered. The establishment of novel flow cytometry techniques to assess antidiabetic properties using in vitro cell culture was achieved. These methods will be useful in the future for the assessment of insulin sensitivity and in the identification of novel compounds that stimulate the insulin signalling pathways.
- Full Text:
- Date Issued: 2010
- Authors: Elliot, Gayle Pamela
- Date: 2010
- Subjects: Insulin resistance -- South Africa , Insulin -- Therapeutic use -- South Africa , Medicinal plants -- South Africa , Non-insulin-dependent diabetes -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:10304 , http://hdl.handle.net/10948/1439 , Insulin resistance -- South Africa , Insulin -- Therapeutic use -- South Africa , Medicinal plants -- South Africa , Non-insulin-dependent diabetes -- South Africa
- Description: The ability of insulin to stimulate glucose uptake into muscle and adipose tissue is central to the maintenance of whole-body glucose homeostasis. Deregulation of insulin action manifests itself as insulin resistance, a key component of type 2 diabetes. Insulin resistance is also observed in HIV patients receiving protease inhibitors. An agent that can reversibly induce an insulin-resistant state would be a very useful tool in developing model systems that mimic the pathogenesis of type 2 diabetes. Insulin resistance can arise from defects in insulin signal transduction, changes in the expression of proteins or genes that are targets of insulin action, cross talk from other hormonal systems or metabolic abnormalities. Deterioration of the insulin-receptor-signalling pathway at different levels leading to decreased levels of signalling pathway intermediates and/or decreased activation through phosphorylation accounts for the evolution from an insulin-resistant state to type 2 diabetes. In addition, defects in GLUT4 glucose transporter translocation are observed, further fuelling impairments in skeletal muscle glucose uptake. Levels of insulin-induced GLUT4 translocation in the skeletal muscle of type 2 diabetic patients are typically reduced by 90%. Many cellular pathways & their intermediates are in some way or another linked to insulin signalling. This study focused on three of these namely the PI3-kinase/Akt pathway, the Mitogen Activated Protein Kinase (MAPK) cascade and the AMP Kinase pathway, with successful monitoring of the PI3-K pathway. Investigations involved observing and evaluating the effects of various compounds as well as an indigenous medicinal plant, Sutherlandia frutescens on the activities of key insulin signalling pathway intermediates within the three fore mentioned pathways including Akt, AMPK and MEK1/2 as well as membrane surface GLUT4 levels. Scientific research has in the past leant heavily on Western blotting as the method of choice for gaining vital information relating to signal transduction pathways, however for research into cellular mechanisms the negatives of this method outweigh the positives. The drawbacks include a need for large amount of cells, multiple washing steps which may be disadvantageous to any weak and transient interactions as well as lysing of cells which may interfere with the maintenance of the subcellular localisation of a specific signalling event. Based on these, the need for a better method in terms of speed & reliability to monitor phosphorylation states of signal transduction pathway intermediates & GLUT4 translocation was evident and was one VII of the main aims & successes of this study. The method created used the mouse muscle cell line C2C12 in conjunction with the quick, sensitive method of flow cytometry which allowed us to monitor these processes in these cells through immune-labelling. Adherent cell cultures such as the C2C12 cell line pose the problem of possible damage to plasma membrane receptors (including insulin receptors) during harvesting to obtain a cell suspension for flow cytometry. We however used C2C12 mouse myocytes to optimize a method yielding insulin responsive cells in suspension that were successfully used for flow cytometry after immunelabelling of insulin signalling intermediates. Insulin (0.1μM) significantly raised the levels of both P-Akt and GLUT4 above basal levels. This effect was shown to be dose dependent. At a concentration of 50μg/ml, Sutherlandia frutescens was able to act as an insulin-mimetic in terms of its ability to increase P-Akt levels, GLUT4 translocation and glucose utilisation in an acute manner. These increases could be reduced with the addition of wortmannin, a PI3-K inhibitor. Therefore, these results suggest the mechanism of the plant extract’s insulin-like activity may be in part due to the activation of the insulin signalling pathway leading to GLUT4 translocation, which involves the phosphorylation of insulin receptor- and subsequent PI3-K activity, leading to P-Akt activity. These results provide further evidence of this plant extract’s anti-diabetic potential. The effect of Sutherlandia frutescens on insulin secretion, calcium signalling and proliferation in INS-1 rat pancreatic cells was also investigated and it was found to increase the activities of all of these processes. However no change in the levels of GLUT2 glucose transporter was seen. Ritonavir is prescribed by the South African Department of Health in co-formulation with other protease inhibitors within its second regime in the treatment of HIV and AIDS. Using C2C12 cells, ritonavir decreased glucose uptake acutely and had no effect on GLUT4 translocation however surprisingly increased P-Akt levels. In conclusion, it was found that Sutherlandia frutescens has antidiabetic benefits, diverse in nature depending on tissue type as well as length of time administered. The establishment of novel flow cytometry techniques to assess antidiabetic properties using in vitro cell culture was achieved. These methods will be useful in the future for the assessment of insulin sensitivity and in the identification of novel compounds that stimulate the insulin signalling pathways.
- Full Text:
- Date Issued: 2010
Integer optimisation for the selection of a fantasy league cricket team
- Authors: Brettenny, Warren James
- Date: 2010
- Subjects: Sports -- Research -- Methodology , Teamwork (Sports) , Cricket players
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10565 , http://hdl.handle.net/10948/1230 , Sports -- Research -- Methodology , Teamwork (Sports) , Cricket players
- Description: Sports fans often scrutinise the team selection strategies employed by their favourite team's coach or selection panel. Many of these fans believe that they can perform the selection process far better than those tasked with the responsibility. Fantasy leagues, provide a platform for fans to test their hand at this selection procedure. Twenty20 cricket is a new and exciting form of cricket and has become very popular in recent years. This research focuses on bringing these concepts together by proposing a binary integer program to determine a team selection strategy for fantasy league cricket. This is done in a Twenty20 setting. The approach used in this study focuses on evaluating the effectiveness of previously developed performance measures in a fantasy league setting. Adjustments to these measures are made and new measures are proposed. These measures are then used to select a fantasy league team using a prospective approach. This is done to provide fantasy league participants with a mathematical procedure for fantasy league team selection.
- Full Text:
- Date Issued: 2010
- Authors: Brettenny, Warren James
- Date: 2010
- Subjects: Sports -- Research -- Methodology , Teamwork (Sports) , Cricket players
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10565 , http://hdl.handle.net/10948/1230 , Sports -- Research -- Methodology , Teamwork (Sports) , Cricket players
- Description: Sports fans often scrutinise the team selection strategies employed by their favourite team's coach or selection panel. Many of these fans believe that they can perform the selection process far better than those tasked with the responsibility. Fantasy leagues, provide a platform for fans to test their hand at this selection procedure. Twenty20 cricket is a new and exciting form of cricket and has become very popular in recent years. This research focuses on bringing these concepts together by proposing a binary integer program to determine a team selection strategy for fantasy league cricket. This is done in a Twenty20 setting. The approach used in this study focuses on evaluating the effectiveness of previously developed performance measures in a fantasy league setting. Adjustments to these measures are made and new measures are proposed. These measures are then used to select a fantasy league team using a prospective approach. This is done to provide fantasy league participants with a mathematical procedure for fantasy league team selection.
- Full Text:
- Date Issued: 2010
Investigating the effect of various film-forming polymers on the evaporation rate of a volatile component in a cosmetic formulation
- Authors: Barnard, Carla
- Date: 2010
- Subjects: Cosmetic delivery systems , Controlled release preparations , Cosmetics , Polymers , Drugs -- Controlled release
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10377 , http://hdl.handle.net/10948/1498 , Cosmetic delivery systems , Controlled release preparations , Cosmetics , Polymers , Drugs -- Controlled release
- Description: The topical application of many substances, including drugs, enzymes, moisturizers and fragrances, contributes largely to the cosmetic and pharmaceutical industries. These components are often volatile in nature and dissipate in a matter of hours. When considering the different types of slow release systems, an overwhelming variety of these systems is available. Each one of the systems is unique in a way, and is designed to perform a particular function, whether it facilitates the controlled release of an active into the body via the skin surface (transdermal delivery) or whether it reduces the rate of loss of an active from the skin surface to the surrounding environment. For the purpose of this study, a previously existing fixative formulation which is believed to reduce the rate of loss of an active component to the environment, through film formation on the skin surface, was investigated. Alternative ingredients or components were incorporated together with the original fixative formulation ingredients into an experimental design which investigates the effect of each group of the components present. 18 formulations with various concentrations of the components within the groups and specified upper and lower limits for each component were formulated. The fixative properties of the formulations were analysed through the incorporation of a fixed amount of a simple fragrance molecule, 4- methoxybenzaldehyde, into each formulation and evaporation studies were conducted in an environmental room at 28±1° C over a period of 5 hours followed by gas chromatography analysis and finally data analyses using statistical methods. The most efficient fixative formulation was established using regression analysis. The fragrance compound in this formulation was found to evaporate at a rate of 0.47 g/L per hour. The least efficient fixative formulation lead to the loss of 0.78 g/L of the fragrance component per hour. From the calculated fragrance concentrations, the rate constant for each individual fixative formulation could be calculated and response surface 8 modelling by backward regression was used in order to determine how each component contributes to the rate of loss of the fragrance compound. Since the sum of the original ingredient and its alternative was constant, each of the original ingredients was coupled directly to its alternative and no conclusion could be made about the contribution of individual components. By increasing the concentration of Hydroxypropylcellulose (HPC) 100K and its alternative HPC 140K, while keeping the effects of the other components constant, a decrease in the rate of fragrance loss was observed. The same conclusion could be made when increasing the concentrations of PEG-12 Dimethicone and its alternative cetyl dimethicone (decreases the evaporation rate). An interaction took place between HPC 100K and PEG-12 dimethicone and their alternatives. The negative effect was, however, not as strong as the combined positive effect on the rate of fragrance loss of the individual components HPC and PEG-12 dimethicone. Evidence suggested that the removal of the components polyvinylpyrrolidone and its alternative, polyurethane-32 (Baycusan® C1003), would improve the effectiveness of the fixative formulation in terms of its slow release properties. A confirmation experiment established that the exclusion of these components from the fixative formulation does improve the “slow release” properties thereof. A larger, more intricate design is required to investigate the effect of each one of the individual components and where the sum of the components (original and its alternative) is not constant.
- Full Text:
- Date Issued: 2010
- Authors: Barnard, Carla
- Date: 2010
- Subjects: Cosmetic delivery systems , Controlled release preparations , Cosmetics , Polymers , Drugs -- Controlled release
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10377 , http://hdl.handle.net/10948/1498 , Cosmetic delivery systems , Controlled release preparations , Cosmetics , Polymers , Drugs -- Controlled release
- Description: The topical application of many substances, including drugs, enzymes, moisturizers and fragrances, contributes largely to the cosmetic and pharmaceutical industries. These components are often volatile in nature and dissipate in a matter of hours. When considering the different types of slow release systems, an overwhelming variety of these systems is available. Each one of the systems is unique in a way, and is designed to perform a particular function, whether it facilitates the controlled release of an active into the body via the skin surface (transdermal delivery) or whether it reduces the rate of loss of an active from the skin surface to the surrounding environment. For the purpose of this study, a previously existing fixative formulation which is believed to reduce the rate of loss of an active component to the environment, through film formation on the skin surface, was investigated. Alternative ingredients or components were incorporated together with the original fixative formulation ingredients into an experimental design which investigates the effect of each group of the components present. 18 formulations with various concentrations of the components within the groups and specified upper and lower limits for each component were formulated. The fixative properties of the formulations were analysed through the incorporation of a fixed amount of a simple fragrance molecule, 4- methoxybenzaldehyde, into each formulation and evaporation studies were conducted in an environmental room at 28±1° C over a period of 5 hours followed by gas chromatography analysis and finally data analyses using statistical methods. The most efficient fixative formulation was established using regression analysis. The fragrance compound in this formulation was found to evaporate at a rate of 0.47 g/L per hour. The least efficient fixative formulation lead to the loss of 0.78 g/L of the fragrance component per hour. From the calculated fragrance concentrations, the rate constant for each individual fixative formulation could be calculated and response surface 8 modelling by backward regression was used in order to determine how each component contributes to the rate of loss of the fragrance compound. Since the sum of the original ingredient and its alternative was constant, each of the original ingredients was coupled directly to its alternative and no conclusion could be made about the contribution of individual components. By increasing the concentration of Hydroxypropylcellulose (HPC) 100K and its alternative HPC 140K, while keeping the effects of the other components constant, a decrease in the rate of fragrance loss was observed. The same conclusion could be made when increasing the concentrations of PEG-12 Dimethicone and its alternative cetyl dimethicone (decreases the evaporation rate). An interaction took place between HPC 100K and PEG-12 dimethicone and their alternatives. The negative effect was, however, not as strong as the combined positive effect on the rate of fragrance loss of the individual components HPC and PEG-12 dimethicone. Evidence suggested that the removal of the components polyvinylpyrrolidone and its alternative, polyurethane-32 (Baycusan® C1003), would improve the effectiveness of the fixative formulation in terms of its slow release properties. A confirmation experiment established that the exclusion of these components from the fixative formulation does improve the “slow release” properties thereof. A larger, more intricate design is required to investigate the effect of each one of the individual components and where the sum of the components (original and its alternative) is not constant.
- Full Text:
- Date Issued: 2010
Leonotis leonurus: the anticoagulant and antidiabetic activity of Leonotis leonurus
- Authors: Mnonopi, Nandipha
- Date: 2010
- Subjects: Medicinal plants -- South Africa , Materia medica, Vegetable -- South Africa , Diabetes -- Alternative treatment -- South Africa , Plant bioactive compounds , Leonotis leonurus -- Physiological aspects
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:10323 , http://hdl.handle.net/10948/1194 , Medicinal plants -- South Africa , Materia medica, Vegetable -- South Africa , Diabetes -- Alternative treatment -- South Africa , Plant bioactive compounds , Leonotis leonurus -- Physiological aspects
- Description: Commercial marrubiin, aqueous and organic extracts of Leonotis leonurus were tested in vitro for their anticoagulant and antiplatelet activities. The aqueous extract inhibited platelet aggregation by 69.5 percent (100 μg/mL), while the organic extract (100 μg/mL) and marrubiin (5 μg/mL) showed 92.5 percent and 91.6 percent inhibition, respectively, by inhibiting the binding of fibrinogen to glycoprotein IIb/IIIa receptor in a concentration dependent manner. The extracts significantly prolonged activated partial thromboplastin time compared to untreated plasma controls. Fibrin and D-Dimer formation were drastically decreased. The extracts and marrubiin concentration-dependently inhibited calcium mobilization induced by collagen and thrombin. The formation of thromboxane A2 was also significantly reduced by both the extracts and marrubiin. Protein secretion and platelet adhesion were significantly reduced by both the extracts and marrubiin. The organic extract and marrubiin showed a more pronounced effect than the aqueous extracts in all the in vitro assays. The ex-vivo animal model confirmed the results obtained in vitro. Similar to the in vitro studies, activated partial thromboplastin time clotting time was prolonged by marrubiin and the number of aggregated platelets were significantly reduced relative to aspirin. The findings reflect that marrubiin largely contributes to the organic extract's anticoagulant and antiplatelet effect in vitro. INS-1 cells were cultured under normo- and hyperglycaemic conditions. Marrubiin and the two Leonotis leonurus extracts were screened for anti-diabetic activity in vitro. The stimulatory index of INS-1 cells cultured under hyperglycaemic conditions was significantly increased by 60 percent and 61 percent (p<0.01; n=5) in cells exposed to the organic extract (10 μg/mL) and marrubiin (500 ng/mL), respectively, relative to the normoglycaemic conditions. The gene expression of insulin was significantly increased by 76.5 and 71 percent, and of glucose transporter-2 by 93 and 92.5 percent for marrubiin and the organic extract, respectively, under the same conditions stipulated above (p<0.01; n=4). The extract and marrubiin similarly showed an increase in respiratory rate under hyperglycaemic conditions. Marrubiin increased insulin secretion, HDL-cholesterol, while it decreased total cholesterol, LDL-cholesterol and the atherogenic index in the in vivo rat model.
- Full Text:
- Date Issued: 2010
- Authors: Mnonopi, Nandipha
- Date: 2010
- Subjects: Medicinal plants -- South Africa , Materia medica, Vegetable -- South Africa , Diabetes -- Alternative treatment -- South Africa , Plant bioactive compounds , Leonotis leonurus -- Physiological aspects
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:10323 , http://hdl.handle.net/10948/1194 , Medicinal plants -- South Africa , Materia medica, Vegetable -- South Africa , Diabetes -- Alternative treatment -- South Africa , Plant bioactive compounds , Leonotis leonurus -- Physiological aspects
- Description: Commercial marrubiin, aqueous and organic extracts of Leonotis leonurus were tested in vitro for their anticoagulant and antiplatelet activities. The aqueous extract inhibited platelet aggregation by 69.5 percent (100 μg/mL), while the organic extract (100 μg/mL) and marrubiin (5 μg/mL) showed 92.5 percent and 91.6 percent inhibition, respectively, by inhibiting the binding of fibrinogen to glycoprotein IIb/IIIa receptor in a concentration dependent manner. The extracts significantly prolonged activated partial thromboplastin time compared to untreated plasma controls. Fibrin and D-Dimer formation were drastically decreased. The extracts and marrubiin concentration-dependently inhibited calcium mobilization induced by collagen and thrombin. The formation of thromboxane A2 was also significantly reduced by both the extracts and marrubiin. Protein secretion and platelet adhesion were significantly reduced by both the extracts and marrubiin. The organic extract and marrubiin showed a more pronounced effect than the aqueous extracts in all the in vitro assays. The ex-vivo animal model confirmed the results obtained in vitro. Similar to the in vitro studies, activated partial thromboplastin time clotting time was prolonged by marrubiin and the number of aggregated platelets were significantly reduced relative to aspirin. The findings reflect that marrubiin largely contributes to the organic extract's anticoagulant and antiplatelet effect in vitro. INS-1 cells were cultured under normo- and hyperglycaemic conditions. Marrubiin and the two Leonotis leonurus extracts were screened for anti-diabetic activity in vitro. The stimulatory index of INS-1 cells cultured under hyperglycaemic conditions was significantly increased by 60 percent and 61 percent (p<0.01; n=5) in cells exposed to the organic extract (10 μg/mL) and marrubiin (500 ng/mL), respectively, relative to the normoglycaemic conditions. The gene expression of insulin was significantly increased by 76.5 and 71 percent, and of glucose transporter-2 by 93 and 92.5 percent for marrubiin and the organic extract, respectively, under the same conditions stipulated above (p<0.01; n=4). The extract and marrubiin similarly showed an increase in respiratory rate under hyperglycaemic conditions. Marrubiin increased insulin secretion, HDL-cholesterol, while it decreased total cholesterol, LDL-cholesterol and the atherogenic index in the in vivo rat model.
- Full Text:
- Date Issued: 2010