Characterisation and cryopreservation of semen from indigenous Namaqua Afrikaner sheep breed, in comparison with Dorper and Dohne Merino breeds
- Authors: Letsoalo, Phutiane Thomas
- Date: 2017
- Subjects: Merino sheep Dorper sheep Semen
- Language: English
- Type: Thesis , Masters , Animal Science
- Identifier: http://hdl.handle.net/10353/11707 , vital:39099
- Description: The aim of this study was to characterise and cryopreserve semen of the indigenous Namaqua Afrikaner breed, and to compare it to that of Dorper and Dohne Merino sheep, whose semen is commercially frozen on a large scale. The study was conducted between January and August 2015. September 2013-born Namaqua Afrikaner (12), Dohne Merino (12) and Dorper (9) rams were used in the study. The rams were kept under kraal conditions with adequate shade, and they received a high protein, high energy diet. Originally it was envisaged to collect semen samples using the artificial vagina (AV) method, which proved to be problematic with the Namaqua Afrikaner rams. Semen samples were subsequently collected twice a week by either AV (Dohne Merino and Dorper) or electro-ejaculation (EE; all three breeds). Macroscopic sperm traits were assessed and sperm concentration determined immediately after collection. Each semen sample was diluted with Triladyl® (1:3) and subsequently frozen in liquid nitrogen vapour in straws. Frozen straws were thawed and evaluated at 7, 30 and 90 days after cryopreservation. A droplet (0.5 ml) from each thawed sample was assessed microscopically for post-thaw motility and percentage live sperm. Nigrosin-Eosin smears were prepared of fresh, diluted and frozen-thawed semen samples for determination of percentage live sperm. Data of all the traits were analysed with the GLM and CHI-SQUARE procedures of the SAS statistical package. Breed had a significant effect on ejaculate volume of fresh semen collected either via electro-ejaculation or artificial vagina. Dorper rams (1.37 ± 0.08 ml) and Dohne Merino rams (1.20 ± 0.08 ml) produced ejaculates with higher (P <0.05) semen volume than Namaqua Afrikaner rams (1.09 ± 0.08 ml) when using the EE. With the AV method, Dohne Merino rams (1.46 ± 0.08 ml) produced a higher (P <0.05) ejaculate volume than Dorper rams (1.22 ± 0.08 ml). Motility of the frozen-thawed semen samples was lower than that of the fresh and diluted samples for all breeds and collection methods. Furthermore, motility 3 hours after thawing was also lower than motility assessed immediately after thawing for all breeds and collection methods. Motility of frozen-thawed semen collected with an AV and evaluated at 7, 30 and 90 days after freezing was significantly higher than that collected via EE. Dorper rams had the lowest sperm concentration (1.10 ± 5.29x109 sperm/ml) and the Namaqua rams the highest sperm concentration (1.22 ± 5.20x109 sperm/ml) (P ˂0.05). The AV samples had a higher (P >0.05) sperm concentration (1.20 ± 3.68x109 sperm/ml) than the EE samples (1.11 ± 3.51x109 sperm/ml). The percentage live sperm in the fresh semen samples did not differ among Namaqua Afrikaner (67.76 ± 1.94percent), Dohne Merino (68.59 ± 1.94percent) and Dorper (72.82 ± 1.98percent) rams. The percentage live sperm for all three breeds dropped considerably after freezing to 17.76 ± 2.03percent, 17.86 ± 2.03percent and 22.72 ± 2.07percent respectively. It remained constant for all the breeds from 7 until 90 days after freezing, indicating that length of storage should not have an effect on percentage live sperm for semen collected via AV or EE. Percentage live sperm of the frozen-thawed semen of the Namaqua rams was lower than that of the Dorper rams, indicating that the Namaqua semen collected via EE did not freeze as well as that of the Dorper semen. In conclusion, neither fresh nor frozen-thawed Dorper and Dohne Merino semen collected via EE did differ significantly. Furthermore, except for semen volume, Dorper and Dohne Merino semen collected via AV did not differ significantly whether evaluated as fresh or frozen-thawed semen. However, both Dorper and Dohne Merino rams produced semen with higher motility and a higher percentage live sperm post-thaw when the semen samples were collected via an AV than via EE. From these results it can therefore be postulated that if Namaqua semen were collected via AV it could have a higher post-thaw percentage live sperm than if semen was collected via EE. Advanced further studies are necessary to investigate the reason for the lower post-thaw survival rate of sperm of the Namaqua Afrikaner rams. This is necessary as semen stored in a cryobank for breeding and conservation purposes for this endangered breed has to be of high quality. Such resources as cryobanks are expensive and funds cannot be wasted on preserving inferior samples that could not fertilize an ovum when needed. The low percentage of live sperm obtained with the frozen-thawed samples in this, as well as other studies on indigenous breeds, is an indication that further research is needed into more suitable freezing protocols. It can be concluded that Namaqua Afrikaner semen cannot be frozen successfully for the purpose of storage in a cryobank, when using a freezing protocol based on Triladyl® as extender. Furthermore, any increase in post-thaw survival rate of sperm will be beneficial and it is therefore suggested that all efforts be made to solve the problem of the Namaqua Afrikaner rams that do not want to ejaculate into an artificial vagina.
- Full Text:
- Date Issued: 2017
- Authors: Letsoalo, Phutiane Thomas
- Date: 2017
- Subjects: Merino sheep Dorper sheep Semen
- Language: English
- Type: Thesis , Masters , Animal Science
- Identifier: http://hdl.handle.net/10353/11707 , vital:39099
- Description: The aim of this study was to characterise and cryopreserve semen of the indigenous Namaqua Afrikaner breed, and to compare it to that of Dorper and Dohne Merino sheep, whose semen is commercially frozen on a large scale. The study was conducted between January and August 2015. September 2013-born Namaqua Afrikaner (12), Dohne Merino (12) and Dorper (9) rams were used in the study. The rams were kept under kraal conditions with adequate shade, and they received a high protein, high energy diet. Originally it was envisaged to collect semen samples using the artificial vagina (AV) method, which proved to be problematic with the Namaqua Afrikaner rams. Semen samples were subsequently collected twice a week by either AV (Dohne Merino and Dorper) or electro-ejaculation (EE; all three breeds). Macroscopic sperm traits were assessed and sperm concentration determined immediately after collection. Each semen sample was diluted with Triladyl® (1:3) and subsequently frozen in liquid nitrogen vapour in straws. Frozen straws were thawed and evaluated at 7, 30 and 90 days after cryopreservation. A droplet (0.5 ml) from each thawed sample was assessed microscopically for post-thaw motility and percentage live sperm. Nigrosin-Eosin smears were prepared of fresh, diluted and frozen-thawed semen samples for determination of percentage live sperm. Data of all the traits were analysed with the GLM and CHI-SQUARE procedures of the SAS statistical package. Breed had a significant effect on ejaculate volume of fresh semen collected either via electro-ejaculation or artificial vagina. Dorper rams (1.37 ± 0.08 ml) and Dohne Merino rams (1.20 ± 0.08 ml) produced ejaculates with higher (P <0.05) semen volume than Namaqua Afrikaner rams (1.09 ± 0.08 ml) when using the EE. With the AV method, Dohne Merino rams (1.46 ± 0.08 ml) produced a higher (P <0.05) ejaculate volume than Dorper rams (1.22 ± 0.08 ml). Motility of the frozen-thawed semen samples was lower than that of the fresh and diluted samples for all breeds and collection methods. Furthermore, motility 3 hours after thawing was also lower than motility assessed immediately after thawing for all breeds and collection methods. Motility of frozen-thawed semen collected with an AV and evaluated at 7, 30 and 90 days after freezing was significantly higher than that collected via EE. Dorper rams had the lowest sperm concentration (1.10 ± 5.29x109 sperm/ml) and the Namaqua rams the highest sperm concentration (1.22 ± 5.20x109 sperm/ml) (P ˂0.05). The AV samples had a higher (P >0.05) sperm concentration (1.20 ± 3.68x109 sperm/ml) than the EE samples (1.11 ± 3.51x109 sperm/ml). The percentage live sperm in the fresh semen samples did not differ among Namaqua Afrikaner (67.76 ± 1.94percent), Dohne Merino (68.59 ± 1.94percent) and Dorper (72.82 ± 1.98percent) rams. The percentage live sperm for all three breeds dropped considerably after freezing to 17.76 ± 2.03percent, 17.86 ± 2.03percent and 22.72 ± 2.07percent respectively. It remained constant for all the breeds from 7 until 90 days after freezing, indicating that length of storage should not have an effect on percentage live sperm for semen collected via AV or EE. Percentage live sperm of the frozen-thawed semen of the Namaqua rams was lower than that of the Dorper rams, indicating that the Namaqua semen collected via EE did not freeze as well as that of the Dorper semen. In conclusion, neither fresh nor frozen-thawed Dorper and Dohne Merino semen collected via EE did differ significantly. Furthermore, except for semen volume, Dorper and Dohne Merino semen collected via AV did not differ significantly whether evaluated as fresh or frozen-thawed semen. However, both Dorper and Dohne Merino rams produced semen with higher motility and a higher percentage live sperm post-thaw when the semen samples were collected via an AV than via EE. From these results it can therefore be postulated that if Namaqua semen were collected via AV it could have a higher post-thaw percentage live sperm than if semen was collected via EE. Advanced further studies are necessary to investigate the reason for the lower post-thaw survival rate of sperm of the Namaqua Afrikaner rams. This is necessary as semen stored in a cryobank for breeding and conservation purposes for this endangered breed has to be of high quality. Such resources as cryobanks are expensive and funds cannot be wasted on preserving inferior samples that could not fertilize an ovum when needed. The low percentage of live sperm obtained with the frozen-thawed samples in this, as well as other studies on indigenous breeds, is an indication that further research is needed into more suitable freezing protocols. It can be concluded that Namaqua Afrikaner semen cannot be frozen successfully for the purpose of storage in a cryobank, when using a freezing protocol based on Triladyl® as extender. Furthermore, any increase in post-thaw survival rate of sperm will be beneficial and it is therefore suggested that all efforts be made to solve the problem of the Namaqua Afrikaner rams that do not want to ejaculate into an artificial vagina.
- Full Text:
- Date Issued: 2017
Characterisation and cryopreservation of semen from indigenous Namaqua Afrikaner sheep breed, in comparison with Dorper and Dohne Merino breeds
- Authors: Letsoalo, Phutiane Thomas
- Date: 2017
- Subjects: Cryopreservation of organs, tissues, etc Merino sheep Dorper sheep
- Language: English
- Type: Thesis , Masters , Degree
- Identifier: http://hdl.handle.net/10353/4759 , vital:28510
- Description: The aim of this study was to characterise and cryopreserve semen of the indigenous Namaqua Afrikaner breed, and to compare it to that of Dorper and Dohne Merino sheep, whose semen is commercially frozen on a large scale. The study was conducted between January and August 2015. September 2013-born Namaqua Afrikaner (12), Dohne Merino (12) and Dorper (9) rams were used in the study. The rams were kept under kraal conditions with adequate shade, and they received a high protein, high energy diet. Originally it was envisaged to collect semen samples using the artificial vagina (AV) method, which proved to be problematic with the Namaqua Afrikaner rams. Semen samples were subsequently collected twice a week by either AV (Dohne Merino and Dorper) or electro-ejaculation (EE; all three breeds). Macroscopic sperm traits were assessed and sperm concentration determined immediately after collection. Each semen sample was diluted with Triladyl® (1:3) and subsequently frozen in liquid nitrogen vapour in straws. Frozen straws were thawed and evaluated at 7, 30 and 90 days after cryopreservation. A droplet (0.5 ml) from each thawed sample was assessed microscopically for post-thaw motility and percentage live sperm..
- Full Text:
- Date Issued: 2017
- Authors: Letsoalo, Phutiane Thomas
- Date: 2017
- Subjects: Cryopreservation of organs, tissues, etc Merino sheep Dorper sheep
- Language: English
- Type: Thesis , Masters , Degree
- Identifier: http://hdl.handle.net/10353/4759 , vital:28510
- Description: The aim of this study was to characterise and cryopreserve semen of the indigenous Namaqua Afrikaner breed, and to compare it to that of Dorper and Dohne Merino sheep, whose semen is commercially frozen on a large scale. The study was conducted between January and August 2015. September 2013-born Namaqua Afrikaner (12), Dohne Merino (12) and Dorper (9) rams were used in the study. The rams were kept under kraal conditions with adequate shade, and they received a high protein, high energy diet. Originally it was envisaged to collect semen samples using the artificial vagina (AV) method, which proved to be problematic with the Namaqua Afrikaner rams. Semen samples were subsequently collected twice a week by either AV (Dohne Merino and Dorper) or electro-ejaculation (EE; all three breeds). Macroscopic sperm traits were assessed and sperm concentration determined immediately after collection. Each semen sample was diluted with Triladyl® (1:3) and subsequently frozen in liquid nitrogen vapour in straws. Frozen straws were thawed and evaluated at 7, 30 and 90 days after cryopreservation. A droplet (0.5 ml) from each thawed sample was assessed microscopically for post-thaw motility and percentage live sperm..
- Full Text:
- Date Issued: 2017
Effects of loading time and lairage duration on pig behaviour, sex and lairage time on pork physio-chemical characteristics
- Authors: Ntlatywa, Bongiswa
- Date: 2017
- Subjects: Pork -- Quality Meat -- Quality Loading and unloading
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/12270 , vital:39225
- Description: The objectives of the study were to determine the effect of loading time, transportation unloading and lairage period, their subsequent effect on responses behaviour and the effect of sex and lairage period on the meat quality of cross breeds reared in commercial farm. The first part of the study assessed the behaviour of pigs during loading at the farm, offloading and in the lairages. A total of 900 pigs from 5 trips (180 per trip) were monitored on behaviour from farm to slaughter plant. The pigs were transported to a commercial abattoir which is 120 km distance, travelling for 2 hours from the farm. The results revealed that time spent in loading pigs was more than when offloading pigs. The frequency of slipping or falling-off in pigs was high (18percent) than voluntary movement (8percent) during loading times (χ2 >0.05) that when off-loading. A significant difference was determined on the avoidance (χ2=0.03), mounting behaviour (χ2 =0.01) and vocal sound (χ2 =0.02) during loading time on each observation day. Furthermore there was no significant difference determined on the time and day of observation in the lairages. These results also reveal that the avoidance behaviour was the highest (47percent) while fighting (34percent) and vocalizing were the least compared to other behaviour during the first 30 minutes after arrival at the abattoir. Moreover, the lying behaviour was found to have highest frequency (15percent) on day 6 compared to other sampling days. These results reveal that time of loading pigs had an influence on pigs’ behaviour before slaughter and consequently that affected physiological features and meat quality. The second section of the study assessed sex and the different lairage time effects on the pork quality attributes. A total of fifty four pigs, 22 weeks old of different sex (26 boars x 28 gilts) reared on a commercial farm and transported to the abattoir on the same day was used in this study. At the abattoir, pigs were grouped according to lairage periods of 17 hours (Group1; 13 gilts and 14 boars) and 36 hours (Group 2; 15 gilts and 12 boars). Results showed no significant difference on the sex of pigs (P >0.05) and meat pH. However, lairage time influenced the thawing loss (P= 0.02) of meat and also the interactions of sex by lairage had a significant difference (P= 0.04) on meat tenderness. There was a positive significant correlation found between sex and meat attributes values were high in boars (r= 0.61) than in gilts (r = 0.49). A negative correlation between a*and WBSF on lairage group1 (r= -0.52) was determined while on group 2 there was a positive correlation between a* and b* (r=0.49). This study suggest that a proper timing on farm feed withdrawal in combination with a sufficient lairage at the slaughter plant may result in better carcass and meat quality traits. Communication among producers and abattoirs is critical when planning the fasting and lairage times in order to elude carcass and technological pork quality complications. To conclude, there is influence caused by time of loading in stressing pigs before transportation and slaughter as aggressive behaviour showed and also lairage time affected meat quality.
- Full Text:
- Date Issued: 2017
- Authors: Ntlatywa, Bongiswa
- Date: 2017
- Subjects: Pork -- Quality Meat -- Quality Loading and unloading
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/12270 , vital:39225
- Description: The objectives of the study were to determine the effect of loading time, transportation unloading and lairage period, their subsequent effect on responses behaviour and the effect of sex and lairage period on the meat quality of cross breeds reared in commercial farm. The first part of the study assessed the behaviour of pigs during loading at the farm, offloading and in the lairages. A total of 900 pigs from 5 trips (180 per trip) were monitored on behaviour from farm to slaughter plant. The pigs were transported to a commercial abattoir which is 120 km distance, travelling for 2 hours from the farm. The results revealed that time spent in loading pigs was more than when offloading pigs. The frequency of slipping or falling-off in pigs was high (18percent) than voluntary movement (8percent) during loading times (χ2 >0.05) that when off-loading. A significant difference was determined on the avoidance (χ2=0.03), mounting behaviour (χ2 =0.01) and vocal sound (χ2 =0.02) during loading time on each observation day. Furthermore there was no significant difference determined on the time and day of observation in the lairages. These results also reveal that the avoidance behaviour was the highest (47percent) while fighting (34percent) and vocalizing were the least compared to other behaviour during the first 30 minutes after arrival at the abattoir. Moreover, the lying behaviour was found to have highest frequency (15percent) on day 6 compared to other sampling days. These results reveal that time of loading pigs had an influence on pigs’ behaviour before slaughter and consequently that affected physiological features and meat quality. The second section of the study assessed sex and the different lairage time effects on the pork quality attributes. A total of fifty four pigs, 22 weeks old of different sex (26 boars x 28 gilts) reared on a commercial farm and transported to the abattoir on the same day was used in this study. At the abattoir, pigs were grouped according to lairage periods of 17 hours (Group1; 13 gilts and 14 boars) and 36 hours (Group 2; 15 gilts and 12 boars). Results showed no significant difference on the sex of pigs (P >0.05) and meat pH. However, lairage time influenced the thawing loss (P= 0.02) of meat and also the interactions of sex by lairage had a significant difference (P= 0.04) on meat tenderness. There was a positive significant correlation found between sex and meat attributes values were high in boars (r= 0.61) than in gilts (r = 0.49). A negative correlation between a*and WBSF on lairage group1 (r= -0.52) was determined while on group 2 there was a positive correlation between a* and b* (r=0.49). This study suggest that a proper timing on farm feed withdrawal in combination with a sufficient lairage at the slaughter plant may result in better carcass and meat quality traits. Communication among producers and abattoirs is critical when planning the fasting and lairage times in order to elude carcass and technological pork quality complications. To conclude, there is influence caused by time of loading in stressing pigs before transportation and slaughter as aggressive behaviour showed and also lairage time affected meat quality.
- Full Text:
- Date Issued: 2017
Effects of Vigna unguiculata and Lablab purpureus on methanogenesis, haematological parameters and the quality of meat from Xhosa Lop Ear goats
- Authors: Washaya, Soul
- Date: 2017
- Subjects: Legumes Forage Forage plants
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10353/9996 , vital:35249
- Description: The objective of the current study was to evaluate the nutritive value of forage legumes Lablab purpureus (Lablab) and Vigna unguiculata (Cowpea) and their effects on intake, growth, blood parameters and meat quality in Xhosa goats. Forages were established at UFH research farm in the summer of the 2014 /15 growing season in blocks measuring 8m width x 16m long for each legume, replicated three times, making a total of four blocks, and eight treatment units for each legume. Samples were collected at three different stages of growth of preanthesis, anthesis and post anthesis for biomass yield determination. A homogenous sub-sample was then oven dried for 72 hours at 60 0C. After drying the forage samples were milled through a 1mm screen and a 20g sample from each stage of growth and forage sample was subjected to proximate analysis, in-vitro digestibility and anti-nutrient evaluation. The in-vitro digestibility experiment was done with rumen liquor of cattle and sheep collected from an abbatoir. Tannins, total phenolics and sapponins were the only anti-nutrients evaluated as they are perceived to influence methanogenesis in ruminat animals. At 50percent flowering, forage legumes were harvested for hay making which was then used in an in-vivo trial to evaluate the nutritive value of forages. This trial was conducted using eighteen one-year-old goats of average weight 14.2±0.24 kg over a period of sixty days. The results showed that biomass yield, which increased with stage of growth, was significantly higher (P < 0.05) for Lablab than Cowpea (7.968t/ha vs 7.105t/ha). Cowpea exhibited higher moisture content (7.72percent), Ash (13.11percent), acid detergent fibre (ADF) (38.42percent) and crude protein (CP) (20.23percent) than Lablab which had 7.50percent, 11.45 percent, 36.17percent and 19percent, respectively. Lablab forage had significantly higher (P < 0.05) fat content (2.41percent), neutral detergent fibre (NDF) (49.27percent) and hemicellulose (13.07percent) than Cowpea which had 2.1percent; 46.91percent and 8.48percent, respectively. The stage of growth significantly (P < 0.05) affected the mineral content of both forages. Lablab showed significantly higher (P < 0.05) values (1.22percent 0.55percent, 2.67percent) than Cowpea (1.00percent, 0.45percent, 2.30percent) for calcium (Ca), phosphorus (P), and potassium (K) respectively. Lablab forage had significantly higher (P < 0.05) tannin (3.345mgCAE/gDM), phenolic (13.47mgGAE/gDM) and saponin (0.75percent) content compared to Cowpea with values of 1.494mgCAEg/DM; 9.402mgGAE/gDM and vs 1.55percent respectively. The tannin content significantly influenced in-vitro digestibility and enteric methane production. The forage type, harvesting stage and inoculum source significantly (P < 0.05) affected the in-vitro digestibility of the forages. The in-vitro dry matter (IVDM) and in-vitro organic matter (IVOM) digestibility was significantly higher (P < 0.05) for Lablab (41.13percent; 39.63percent) than Cowpea (39.86percent; 19.44percent), respectively. Both forages recorded higher dry matter (DM) digestibility coefficients at anthesis stage of growth 61.58percent, and 51.5 percent for Lablab and Cowpea respectively. The IVOM digestibility increased with stage of harvest (23.7percent to 25.57percent) for Cowpea while Lablab showed a decrease (44.6 percent to 43.9percent) when cattle inoculum was used. The IVDM digestibility was higher (P < 0.05) for Lablab (50.5percent) when cattle inoculum was used while Cowpea was observed to have higher digestibility co-efficiencies (38.54percent) when sheep inoculum was used. Cowpea had lower (P < 0.05) IVOM digestibility than Lablab (19.44percent) vs. 39.6percent). Lablab exhibited higher (P < 0.05) metabolisable energy (ME) values than Cowpea (6.21 vs. 2.70 MJ/kgDM). In the feeding study, the total DM intake by goats for Lablab was higher (P < 0.05) compared to Cowpea (688.1 vs. 607.8 g/head/ day). There was a positive (P < 0.05) correlation between dry matter intake and average daily gain (r= 0.8513, and r=0.5279) for Cowpea and Lablab respectively. Lablab and Cowpea promoted growth of Xhosa goats at a rate of 35.0g/d; and 38.3g/d, respectively which was not significantly different (P > 0.05). However, this growth was associated with a significantly higher (P < 0.05) food conversion ratio (FCR) of 19.16 vs 18.11 for Cowpea and Lablab, respectively. The apparent DM, non structural carbohydrate (NSC) nutrient digestibilities were significantly different (P < 0.05) for Lablab and Cowpea. Furthermore sex of animal influenced significantly (P < 0.05) the DM, ADF, NSC and NDF digestibilities. Lablab and Cowpea did not influence (P > 0.05) individual VFA molar concentrations in both sexes. However, animals fed Lablab had significantly higher butyrate concentrations (P < 0.05). Lablab and Cowpea forages significantly lowered (P > 0.05) enteric methane production in goats compared to a concentrate diet, and this was related to animal activity. Animals produced significantly more (P < 0.05) methane gas when ruminating than feeding or just standing. The average methane produced each year was lower but not significant (P > 0.05) for animals fed Cowpea than Lablab (4.602kg CH4, vs. 4.719kg CH4) when expressed per year. Male animals produced more gas than females with values of 17.40L/day; 12.46 g/kg DMI and 0.126g/day vs. 15.47L/day, 12.28 g/kg DMI, and 0.0109g/day, respectively. Forage legumes had no significant effects (P > 0.05) on serum total proteins (TP), globulin, urea, creatinine, alanine aminotransferase (ALT), alkaline phosphatase (ALP), and gamma- glutamyl transferase (GGT) concentrations, but serum concentration of albumin was influenced by the diets (P < 0.05). Sex of animals significantly influenced ALP concentration (P < 0.05) with male animals exhibiting higer ALP values than females. Measured over time, the plasma concentration of urea and ALP significantly increased (P < 0.05) while creatinine and cholesterol concentration decreased (P < 0.05). Although the overall serum concentration of urea, total proteins, globulin, creatinine, ALT, ALP and GGT remained unchanged among the treatments and between sexes; treatment by sex, treatment by day and sex by day interactions were significant (P < 0.05). The white blood cell (WBC), red blood cell (RBC), mean corpuscular haemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC), haemoglobin, haematocrite, platelet and mean platelet volume (MPV) levels were similar among treatments and between sexes (P > 0.05). The WBC decreased with time by 8.07 x10 9/l while mean crepuscular volume (MCV) increased by 11.83(fl) from day 15 to day 55. Treatment diets significantly (P < 0.05) influenced neutrophile (percent) content while sex influenced basophile (percent) content. There were no treatment or sex effects (P > 0.05) on lymphocytes (percent), monocytes (percent) and eosinophiles (percent). In the meat quality study, the dietary treatments and sex had a significant (P < 0.05) effect on dressing out percent (DPpercent), initial pH, redness (a*), yellowness (b*), Warner Blartze Shear force (WBS) , drip loss and cooking loss in the chevon from Xhosa goats. Compared with a commercial pellet diet, Lablab and Cowpea diets resulted in similar slaughter body weights (SBW), hot carcass weight (HCW) and cold dressed mass (CDM) of carcasses. However, female animals exhibited a significantly (P < 0.05) higher DPpercent than male animals (42.3percent vs. 38.9percent) for all treatments. The male animals exhibited significantly (P < 0.05) higher pH values one hour after slaughter than females (5.79 vs 5.67). Neverthreless the diet and gender did not significantly (P > 0.05) affect the ultimate pH (pHu). Meat samples from male animals had significantly (P < 0.05) higher redness values than females. Feeding cowpea significantly (P < 0.05) increased meat redness with the least drip loss of chevon. Female animals produced significantly (P < 0.05) tougher chevon compared to male animals; 4.16kg and 3.36kg for female and male, respectively. Lablab and Cowpea posses high CP levels and moderately lower NDF with high ADF values that influences dry matter intake (DMI), digestibility and growth of goats. These forage legumes meet animal requirements for fat, ADF, NDF, CP, Ca, Mn, zinc and Cu and their biomass yield is satisfactory.
- Full Text:
- Date Issued: 2017
- Authors: Washaya, Soul
- Date: 2017
- Subjects: Legumes Forage Forage plants
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10353/9996 , vital:35249
- Description: The objective of the current study was to evaluate the nutritive value of forage legumes Lablab purpureus (Lablab) and Vigna unguiculata (Cowpea) and their effects on intake, growth, blood parameters and meat quality in Xhosa goats. Forages were established at UFH research farm in the summer of the 2014 /15 growing season in blocks measuring 8m width x 16m long for each legume, replicated three times, making a total of four blocks, and eight treatment units for each legume. Samples were collected at three different stages of growth of preanthesis, anthesis and post anthesis for biomass yield determination. A homogenous sub-sample was then oven dried for 72 hours at 60 0C. After drying the forage samples were milled through a 1mm screen and a 20g sample from each stage of growth and forage sample was subjected to proximate analysis, in-vitro digestibility and anti-nutrient evaluation. The in-vitro digestibility experiment was done with rumen liquor of cattle and sheep collected from an abbatoir. Tannins, total phenolics and sapponins were the only anti-nutrients evaluated as they are perceived to influence methanogenesis in ruminat animals. At 50percent flowering, forage legumes were harvested for hay making which was then used in an in-vivo trial to evaluate the nutritive value of forages. This trial was conducted using eighteen one-year-old goats of average weight 14.2±0.24 kg over a period of sixty days. The results showed that biomass yield, which increased with stage of growth, was significantly higher (P < 0.05) for Lablab than Cowpea (7.968t/ha vs 7.105t/ha). Cowpea exhibited higher moisture content (7.72percent), Ash (13.11percent), acid detergent fibre (ADF) (38.42percent) and crude protein (CP) (20.23percent) than Lablab which had 7.50percent, 11.45 percent, 36.17percent and 19percent, respectively. Lablab forage had significantly higher (P < 0.05) fat content (2.41percent), neutral detergent fibre (NDF) (49.27percent) and hemicellulose (13.07percent) than Cowpea which had 2.1percent; 46.91percent and 8.48percent, respectively. The stage of growth significantly (P < 0.05) affected the mineral content of both forages. Lablab showed significantly higher (P < 0.05) values (1.22percent 0.55percent, 2.67percent) than Cowpea (1.00percent, 0.45percent, 2.30percent) for calcium (Ca), phosphorus (P), and potassium (K) respectively. Lablab forage had significantly higher (P < 0.05) tannin (3.345mgCAE/gDM), phenolic (13.47mgGAE/gDM) and saponin (0.75percent) content compared to Cowpea with values of 1.494mgCAEg/DM; 9.402mgGAE/gDM and vs 1.55percent respectively. The tannin content significantly influenced in-vitro digestibility and enteric methane production. The forage type, harvesting stage and inoculum source significantly (P < 0.05) affected the in-vitro digestibility of the forages. The in-vitro dry matter (IVDM) and in-vitro organic matter (IVOM) digestibility was significantly higher (P < 0.05) for Lablab (41.13percent; 39.63percent) than Cowpea (39.86percent; 19.44percent), respectively. Both forages recorded higher dry matter (DM) digestibility coefficients at anthesis stage of growth 61.58percent, and 51.5 percent for Lablab and Cowpea respectively. The IVOM digestibility increased with stage of harvest (23.7percent to 25.57percent) for Cowpea while Lablab showed a decrease (44.6 percent to 43.9percent) when cattle inoculum was used. The IVDM digestibility was higher (P < 0.05) for Lablab (50.5percent) when cattle inoculum was used while Cowpea was observed to have higher digestibility co-efficiencies (38.54percent) when sheep inoculum was used. Cowpea had lower (P < 0.05) IVOM digestibility than Lablab (19.44percent) vs. 39.6percent). Lablab exhibited higher (P < 0.05) metabolisable energy (ME) values than Cowpea (6.21 vs. 2.70 MJ/kgDM). In the feeding study, the total DM intake by goats for Lablab was higher (P < 0.05) compared to Cowpea (688.1 vs. 607.8 g/head/ day). There was a positive (P < 0.05) correlation between dry matter intake and average daily gain (r= 0.8513, and r=0.5279) for Cowpea and Lablab respectively. Lablab and Cowpea promoted growth of Xhosa goats at a rate of 35.0g/d; and 38.3g/d, respectively which was not significantly different (P > 0.05). However, this growth was associated with a significantly higher (P < 0.05) food conversion ratio (FCR) of 19.16 vs 18.11 for Cowpea and Lablab, respectively. The apparent DM, non structural carbohydrate (NSC) nutrient digestibilities were significantly different (P < 0.05) for Lablab and Cowpea. Furthermore sex of animal influenced significantly (P < 0.05) the DM, ADF, NSC and NDF digestibilities. Lablab and Cowpea did not influence (P > 0.05) individual VFA molar concentrations in both sexes. However, animals fed Lablab had significantly higher butyrate concentrations (P < 0.05). Lablab and Cowpea forages significantly lowered (P > 0.05) enteric methane production in goats compared to a concentrate diet, and this was related to animal activity. Animals produced significantly more (P < 0.05) methane gas when ruminating than feeding or just standing. The average methane produced each year was lower but not significant (P > 0.05) for animals fed Cowpea than Lablab (4.602kg CH4, vs. 4.719kg CH4) when expressed per year. Male animals produced more gas than females with values of 17.40L/day; 12.46 g/kg DMI and 0.126g/day vs. 15.47L/day, 12.28 g/kg DMI, and 0.0109g/day, respectively. Forage legumes had no significant effects (P > 0.05) on serum total proteins (TP), globulin, urea, creatinine, alanine aminotransferase (ALT), alkaline phosphatase (ALP), and gamma- glutamyl transferase (GGT) concentrations, but serum concentration of albumin was influenced by the diets (P < 0.05). Sex of animals significantly influenced ALP concentration (P < 0.05) with male animals exhibiting higer ALP values than females. Measured over time, the plasma concentration of urea and ALP significantly increased (P < 0.05) while creatinine and cholesterol concentration decreased (P < 0.05). Although the overall serum concentration of urea, total proteins, globulin, creatinine, ALT, ALP and GGT remained unchanged among the treatments and between sexes; treatment by sex, treatment by day and sex by day interactions were significant (P < 0.05). The white blood cell (WBC), red blood cell (RBC), mean corpuscular haemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC), haemoglobin, haematocrite, platelet and mean platelet volume (MPV) levels were similar among treatments and between sexes (P > 0.05). The WBC decreased with time by 8.07 x10 9/l while mean crepuscular volume (MCV) increased by 11.83(fl) from day 15 to day 55. Treatment diets significantly (P < 0.05) influenced neutrophile (percent) content while sex influenced basophile (percent) content. There were no treatment or sex effects (P > 0.05) on lymphocytes (percent), monocytes (percent) and eosinophiles (percent). In the meat quality study, the dietary treatments and sex had a significant (P < 0.05) effect on dressing out percent (DPpercent), initial pH, redness (a*), yellowness (b*), Warner Blartze Shear force (WBS) , drip loss and cooking loss in the chevon from Xhosa goats. Compared with a commercial pellet diet, Lablab and Cowpea diets resulted in similar slaughter body weights (SBW), hot carcass weight (HCW) and cold dressed mass (CDM) of carcasses. However, female animals exhibited a significantly (P < 0.05) higher DPpercent than male animals (42.3percent vs. 38.9percent) for all treatments. The male animals exhibited significantly (P < 0.05) higher pH values one hour after slaughter than females (5.79 vs 5.67). Neverthreless the diet and gender did not significantly (P > 0.05) affect the ultimate pH (pHu). Meat samples from male animals had significantly (P < 0.05) higher redness values than females. Feeding cowpea significantly (P < 0.05) increased meat redness with the least drip loss of chevon. Female animals produced significantly (P < 0.05) tougher chevon compared to male animals; 4.16kg and 3.36kg for female and male, respectively. Lablab and Cowpea posses high CP levels and moderately lower NDF with high ADF values that influences dry matter intake (DMI), digestibility and growth of goats. These forage legumes meet animal requirements for fat, ADF, NDF, CP, Ca, Mn, zinc and Cu and their biomass yield is satisfactory.
- Full Text:
- Date Issued: 2017
Forage production, nutritive value and intake rates by goats of pigeon pea (Cajanus cajan (L.) forages grown in the subtropical region of South Africa
- Authors: Buthelezi, Lindokuhle Sizwe
- Date: 2017
- Subjects: Forage Pigeon pea Forage plants
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/7852 , vital:30773
- Description: Different growth stages and drying methods were used to assess forage production, nutritive value and intake of leaves from three Cajanus cajan varieties (ICEAP 00557, ICEAP 01514 and CIMMYT 100/01). In the 2016/17 growing season, three complete randomized block designs (CRBD’s) were conducted. Regrowth parameters were measured during both the both vegetative and flowering stages. The plots were harvested at the flowering stage. Biomass yields were measured at the flowering stage. The harvested leaves were then dried under the two different drying methods. The methods used were shade- and oven-drying at 60 oC. This was used to determine dry matter (DM), crude protein (CP), neutral detergent fibre (NDF), acid detergent fibre (ADF), acid detergent lignin (ADL) and total non-structural carbohydrates (TNC) for the C. cajan varieties. Minerals Ca, Mg, K, Na, K/(Ca+Mg), P and Zn, Cu, Mn and Fe and phytochemicals condensed tannins, phenolic compounds and saponins were also analyzed. The intake rates and in vitro and in sacco ruminal degradabilities were also determined. The survival percentage was significantly different (P<0.05) only across the means of varieties. There was no significant difference (P>0.05) in the number of shoots per plant, stems per shoot and coppice stems produced by the three C. cajan varieties at vegetative and flowering stage. There was a significant difference (P<0.05) in the heights of plants recorded by the varieties only at flowering stage. The yields of fresh biomass in C. cajan was significantly different (P<0.05) across all the varieties harvested in 2016. In all the varieties there was a significant difference (P<0.05) in dry matter yield in both seasons of 2016 and 2017. The effect of browse varieties and drying methods showed a significant effect (P<0.05) on DM, CP, fat, NDIN, ADIN and hemicellulose content of varieties. There was little impact by varieties and drying methods (P<0.05) on ash, NDF, ADF, ADL, TNC and cellulose content of varieties. Varieties and drying method had no effect (P>0.05) on Ca, Mg and K except for Na and P. There was no effect caused by varieties and drying methods (P>0.05) on Zn and Mn with Cu and Fe being the exceptions. Oven drying showed an overall reduction in the tannin concentration on leaves while there was an increase in saponins. The different varieties had no effect (P>0.05) on the IIR, 0.0275±0.0089, 0.0275±0.0077 and 0.0409±0.0109 for Cv1, Cv2 and Cv3, respectively. The day of the trial and browse variety had an effect (P<0.05) on bite rate. However, the day of trial had no significant effect (P>0.05) on both bite size and the instantaneous intake rate. Individual goat had a significant effect (P<0.05) on bite rate. There was a significant difference (P<0.05) in the in vitro and in sacco rapidly degradable fractions (a) of all the three varieties but the drying method had no effect (P>0.05). The slowly degradable fraction (b) and degradation rate constant (c) of all varieties was not significantly different (P>0.05) on both drying methods during IV DaisyII procedure. However, b and c, in all the varieties was significantly different (P<0.05) in both drying methods during the in sacco procedure. There was no significant difference (P>0.05) in the mean potentially degradable fraction (a+b) across all C. cajan varieties during IV DaisyII procedure, however a+c of all varieties was significantly different (P<0.05) in both the drying methods during the in sacco procedure. The effective in vitro and in sacco degradability of the varieties was significantly different (P<0.05) with a rumen outflow rate of 2, 5 and 8 percent per hour. The shade-dried varieties had a higher (P<0.05) ED than the oven-dried materials during IV DaisyII and the in sacco procedures. It was concluded that the decline in yield of C. cajan during the study is a reflection of poor survival, the insufficient regeneration from seed, nutrient depletion over time and the extended dry seasons. Therefore, shade drying is commended method of drying forages.
- Full Text:
- Date Issued: 2017
- Authors: Buthelezi, Lindokuhle Sizwe
- Date: 2017
- Subjects: Forage Pigeon pea Forage plants
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/7852 , vital:30773
- Description: Different growth stages and drying methods were used to assess forage production, nutritive value and intake of leaves from three Cajanus cajan varieties (ICEAP 00557, ICEAP 01514 and CIMMYT 100/01). In the 2016/17 growing season, three complete randomized block designs (CRBD’s) were conducted. Regrowth parameters were measured during both the both vegetative and flowering stages. The plots were harvested at the flowering stage. Biomass yields were measured at the flowering stage. The harvested leaves were then dried under the two different drying methods. The methods used were shade- and oven-drying at 60 oC. This was used to determine dry matter (DM), crude protein (CP), neutral detergent fibre (NDF), acid detergent fibre (ADF), acid detergent lignin (ADL) and total non-structural carbohydrates (TNC) for the C. cajan varieties. Minerals Ca, Mg, K, Na, K/(Ca+Mg), P and Zn, Cu, Mn and Fe and phytochemicals condensed tannins, phenolic compounds and saponins were also analyzed. The intake rates and in vitro and in sacco ruminal degradabilities were also determined. The survival percentage was significantly different (P<0.05) only across the means of varieties. There was no significant difference (P>0.05) in the number of shoots per plant, stems per shoot and coppice stems produced by the three C. cajan varieties at vegetative and flowering stage. There was a significant difference (P<0.05) in the heights of plants recorded by the varieties only at flowering stage. The yields of fresh biomass in C. cajan was significantly different (P<0.05) across all the varieties harvested in 2016. In all the varieties there was a significant difference (P<0.05) in dry matter yield in both seasons of 2016 and 2017. The effect of browse varieties and drying methods showed a significant effect (P<0.05) on DM, CP, fat, NDIN, ADIN and hemicellulose content of varieties. There was little impact by varieties and drying methods (P<0.05) on ash, NDF, ADF, ADL, TNC and cellulose content of varieties. Varieties and drying method had no effect (P>0.05) on Ca, Mg and K except for Na and P. There was no effect caused by varieties and drying methods (P>0.05) on Zn and Mn with Cu and Fe being the exceptions. Oven drying showed an overall reduction in the tannin concentration on leaves while there was an increase in saponins. The different varieties had no effect (P>0.05) on the IIR, 0.0275±0.0089, 0.0275±0.0077 and 0.0409±0.0109 for Cv1, Cv2 and Cv3, respectively. The day of the trial and browse variety had an effect (P<0.05) on bite rate. However, the day of trial had no significant effect (P>0.05) on both bite size and the instantaneous intake rate. Individual goat had a significant effect (P<0.05) on bite rate. There was a significant difference (P<0.05) in the in vitro and in sacco rapidly degradable fractions (a) of all the three varieties but the drying method had no effect (P>0.05). The slowly degradable fraction (b) and degradation rate constant (c) of all varieties was not significantly different (P>0.05) on both drying methods during IV DaisyII procedure. However, b and c, in all the varieties was significantly different (P<0.05) in both drying methods during the in sacco procedure. There was no significant difference (P>0.05) in the mean potentially degradable fraction (a+b) across all C. cajan varieties during IV DaisyII procedure, however a+c of all varieties was significantly different (P<0.05) in both the drying methods during the in sacco procedure. The effective in vitro and in sacco degradability of the varieties was significantly different (P<0.05) with a rumen outflow rate of 2, 5 and 8 percent per hour. The shade-dried varieties had a higher (P<0.05) ED than the oven-dried materials during IV DaisyII and the in sacco procedures. It was concluded that the decline in yield of C. cajan during the study is a reflection of poor survival, the insufficient regeneration from seed, nutrient depletion over time and the extended dry seasons. Therefore, shade drying is commended method of drying forages.
- Full Text:
- Date Issued: 2017
How phase feeding manipulation affects growth, performance, feed cost, carcass characteristics and the quality of meat from broilers
- Authors: Sokanyile, Sanda
- Date: 2017
- Subjects: Animal nutrition , Feeds , Broilers (Chickens)
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/5565 , vital:29334
- Description: The main objective of the study was to determine how phase feeding manipulation affects growth performance, feed cost, carcass characteristics and quality of meat from broiler chickens. A total of 180 day old un-sexed broiler chicks (Cobb 500) were separated into 3 dietary treatments of 60 birds per treatment and each treatment was replicated 3 times with 20 birds per replicate. The dietary treatments were as follows: T1 (starter 1-7 days; grower 8-21 days; finisher 22-35 days), T2 (starter 1-14 days; grower 15-21 days, finisher 22-35 days) and T3 (starter 1-14 days; grower 15-28 days; finisher 29-35 days). Feed intake and body weight gains were recorded weekly in kilograms. Average daily gain and feed conversion ratio were computed weekly. At day 35, the chickens were slaughtered and 12 representative breast muscles from each treatment were used for the determination of muscle pH (pH1, pH24 and pH48) and colour coordinates (Lightness – L*, redness – a* and yellowness – b*) in triplicate at 1 hour, 24 hours and 48 hours post-mortem.Feed intake (FI), feed conversion ratio (FCR), average daily gain (ADG) and average body weight at slaughter (ABW) were not significant (P>0.05) amongst the treatments. The most cost efficient feed (which accumulated the cheapest cost feed per kg were) T1 (R11.32) and T3 (R11.32) although there was a slight difference in the gross profit of these treatments T1 (R45.71) and T3 (R44.48). Carcass characteristics were the same (P > 0.05) across the treatments except for the wing, thigh, drumstick and the breast (P < 0.05). T2 had the highest wing weight (166.63±8.60), T1 (113.03±8.60) and the lowest in T3 (74.46±8.60). The thigh weight were greater (P<0.05) in T2 (185.69±4.34); T1 (185.54±4.34) compared to T3 (166.97±4.34) which was lower. Treatments 1 (204.17±6.57), T2 (197±6.57) had heavier (P<0.05) breast weights than T3 (186.06±6.57). Dietary treatments had no significant effect (P > 0.05) on pH of the breast meat. No differences were observed in colour during the 1 hour period after slaughter. At 24 hours after slaughter, the L* values were different (P < 0.05) in T1 and T2 (44.3 ± 0.37; 43.7 ± 0.37), respectively, T1 and T3 were the same. The a*-values were different (P < 0.05) at 48 hours after slaughter for T1 and T3 (4.5 ± 0.27; 3.4 ± 0.27), T2 was the same as T1. It was therefore, concluded that since manipulation of starter phase did not have adverse effects on growth performance, gross profit, slight statistical difference in meat quality attributes and carcass characteristics. Therefore, the manipulation of starter diet has the potential to be used in broiler production.
- Full Text:
- Date Issued: 2017
- Authors: Sokanyile, Sanda
- Date: 2017
- Subjects: Animal nutrition , Feeds , Broilers (Chickens)
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/5565 , vital:29334
- Description: The main objective of the study was to determine how phase feeding manipulation affects growth performance, feed cost, carcass characteristics and quality of meat from broiler chickens. A total of 180 day old un-sexed broiler chicks (Cobb 500) were separated into 3 dietary treatments of 60 birds per treatment and each treatment was replicated 3 times with 20 birds per replicate. The dietary treatments were as follows: T1 (starter 1-7 days; grower 8-21 days; finisher 22-35 days), T2 (starter 1-14 days; grower 15-21 days, finisher 22-35 days) and T3 (starter 1-14 days; grower 15-28 days; finisher 29-35 days). Feed intake and body weight gains were recorded weekly in kilograms. Average daily gain and feed conversion ratio were computed weekly. At day 35, the chickens were slaughtered and 12 representative breast muscles from each treatment were used for the determination of muscle pH (pH1, pH24 and pH48) and colour coordinates (Lightness – L*, redness – a* and yellowness – b*) in triplicate at 1 hour, 24 hours and 48 hours post-mortem.Feed intake (FI), feed conversion ratio (FCR), average daily gain (ADG) and average body weight at slaughter (ABW) were not significant (P>0.05) amongst the treatments. The most cost efficient feed (which accumulated the cheapest cost feed per kg were) T1 (R11.32) and T3 (R11.32) although there was a slight difference in the gross profit of these treatments T1 (R45.71) and T3 (R44.48). Carcass characteristics were the same (P > 0.05) across the treatments except for the wing, thigh, drumstick and the breast (P < 0.05). T2 had the highest wing weight (166.63±8.60), T1 (113.03±8.60) and the lowest in T3 (74.46±8.60). The thigh weight were greater (P<0.05) in T2 (185.69±4.34); T1 (185.54±4.34) compared to T3 (166.97±4.34) which was lower. Treatments 1 (204.17±6.57), T2 (197±6.57) had heavier (P<0.05) breast weights than T3 (186.06±6.57). Dietary treatments had no significant effect (P > 0.05) on pH of the breast meat. No differences were observed in colour during the 1 hour period after slaughter. At 24 hours after slaughter, the L* values were different (P < 0.05) in T1 and T2 (44.3 ± 0.37; 43.7 ± 0.37), respectively, T1 and T3 were the same. The a*-values were different (P < 0.05) at 48 hours after slaughter for T1 and T3 (4.5 ± 0.27; 3.4 ± 0.27), T2 was the same as T1. It was therefore, concluded that since manipulation of starter phase did not have adverse effects on growth performance, gross profit, slight statistical difference in meat quality attributes and carcass characteristics. Therefore, the manipulation of starter diet has the potential to be used in broiler production.
- Full Text:
- Date Issued: 2017
Seasonal variations in tick loads, coat characteristics, temperature-humidity index and blood metabolite profiles of extensively raised Boran cows
- Authors: Ntilini, Wonga John
- Date: 2017
- Subjects: Boran cattle -- South Africa Ticks -- South Africa
- Language: English
- Type: Thesis , Masters , MAgric
- Identifier: http://hdl.handle.net/10353/13471 , vital:39672
- Description: This study sought to determine the seasonal variations in tick loads, coat characteristics, temperature-humidity index and blood metabolite profiles of Boran cows reared in arid rangelands of South Africa. A total of 20 Boran cows of different age groups were used in this study. Tick counts, hair length, coat score, average temperature and relative humidity readings were measured twice (beginning and end) in each season (post-rainy, cold-dry, hot-dry, hot-wet). Blood samples were taken seasonally (beginning and end of the season) for the analysis of creatine kinase (CK) activity and haematological parameters (HP). Ticks were observed and counted from different anatomical sites, namely anterior (neck, head, ears, and around eyes), ventral (belly, udder, and limbs) and posterior (back and under the tail). The tick species observed from the Boran cows were Rhipicephalus evertsi evertsi (34.58percent), Amblyomma hebreaum (43.35percent), Rhipicephalus simus (4.48percent) and Rhipicephalus (Boophilus) decoloratus (17.59percent). Significant differences were observed in tick burdens at various anatomical sites, with posterior position having the highest tick loads during the post-rainy, cold-dry and hot-dry season. While the ventral position recorded the highest tick loads during the hot-wet season. It was also observed that the cattle had significantly long hair lengths during the cold-dry season. Furthermore, coat score and hair length were observed to be negatively (P<0.05) correlated with tick counts, while coat scores were positively (P<0.05) correlated with hair length. Significant differences were observed in CK activity across season, with higher levels during the hot-dry and hot-wet seasons compared to the post-rainy and cold-dry seasons. Boran cows were exposed to a mild stress condition during the hot-dry and hot-wet season when the THI values were high (>70). Seasonal variations had an effect (P<0.05) on some HP (HB, HCT, MCV, MCH, EOS, and BAS). Neutrophils (-0.267) and basophils (0.268) were significantly correlated with THI. In conclusion, the Boran cows were more susceptible to tick loads during the hot seasons. Likewise, the levels of creatine kinase activity and temperature-humidity index were high during the hot-dry and hot-wet season. Therefore, seasonal variations had an effect on tick loads, coat characteristics, temperature-humidity index and blood metabolite profiles of extensively raised Boran cows.
- Full Text:
- Date Issued: 2017
- Authors: Ntilini, Wonga John
- Date: 2017
- Subjects: Boran cattle -- South Africa Ticks -- South Africa
- Language: English
- Type: Thesis , Masters , MAgric
- Identifier: http://hdl.handle.net/10353/13471 , vital:39672
- Description: This study sought to determine the seasonal variations in tick loads, coat characteristics, temperature-humidity index and blood metabolite profiles of Boran cows reared in arid rangelands of South Africa. A total of 20 Boran cows of different age groups were used in this study. Tick counts, hair length, coat score, average temperature and relative humidity readings were measured twice (beginning and end) in each season (post-rainy, cold-dry, hot-dry, hot-wet). Blood samples were taken seasonally (beginning and end of the season) for the analysis of creatine kinase (CK) activity and haematological parameters (HP). Ticks were observed and counted from different anatomical sites, namely anterior (neck, head, ears, and around eyes), ventral (belly, udder, and limbs) and posterior (back and under the tail). The tick species observed from the Boran cows were Rhipicephalus evertsi evertsi (34.58percent), Amblyomma hebreaum (43.35percent), Rhipicephalus simus (4.48percent) and Rhipicephalus (Boophilus) decoloratus (17.59percent). Significant differences were observed in tick burdens at various anatomical sites, with posterior position having the highest tick loads during the post-rainy, cold-dry and hot-dry season. While the ventral position recorded the highest tick loads during the hot-wet season. It was also observed that the cattle had significantly long hair lengths during the cold-dry season. Furthermore, coat score and hair length were observed to be negatively (P<0.05) correlated with tick counts, while coat scores were positively (P<0.05) correlated with hair length. Significant differences were observed in CK activity across season, with higher levels during the hot-dry and hot-wet seasons compared to the post-rainy and cold-dry seasons. Boran cows were exposed to a mild stress condition during the hot-dry and hot-wet season when the THI values were high (>70). Seasonal variations had an effect (P<0.05) on some HP (HB, HCT, MCV, MCH, EOS, and BAS). Neutrophils (-0.267) and basophils (0.268) were significantly correlated with THI. In conclusion, the Boran cows were more susceptible to tick loads during the hot seasons. Likewise, the levels of creatine kinase activity and temperature-humidity index were high during the hot-dry and hot-wet season. Therefore, seasonal variations had an effect on tick loads, coat characteristics, temperature-humidity index and blood metabolite profiles of extensively raised Boran cows.
- Full Text:
- Date Issued: 2017
Species composition and seasonal population dynamics of free-living and engorged cattle ticks in three agro-ecological zones of the Eastern Cape Province, South Africa
- Authors: Yawa, Mandla
- Date: 2017
- Subjects: Ticks Boophilus microplus
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/9067 , vital:34224
- Description: This study was conducted to determine species composition and seasonal population dynamics of free-living and engorged cattle ticks in three agro-ecological zones (Bedford dry grassland, Kowie thicket and Bisho thornveld) of the Eastern Cape Province, South Africa. The occurrence of ticks on cattle was studied monthly from April 2016 and March 2017. Tick collections were performed on 360 selected cattle in each agro-ecological zones. During tick collections, 10 randomly selected cattle were sampled at each locality and more attention was given to African blue tick and the presence of Asian blue tick. A half body tick collection was performed from cattle and visible ticks were collected by means of fine-forceps after the animals had been restrained. Body regions that were examined includes the ear, head, neck, chest, abdomen, flank, front and hind leg and feet, tail, and peri-anal region. At each locality, six replicate drags of 100 m, approximately 50 m apart, were performed to collect ticks from vegetation. All ticks collected per cattle and from vegetation were stored in labelled sample tubes containing 70percent ethanol, subsequently counted and identified to genus and species level using a standard stereomicroscope. A total of 31425 ticks belonging to ten species were collected during the study. Rhipicephalus (Boophilus) decoloratus, was the most observed tick species (32.50percent), all other ticks like R. evertsi evertsi (18.84percent), R. appendiculatus (17.26percent), A. hebraeum (16.27percent), R. simus (7.69percent) were also commonly collected. Ixodes pilosus (3.84percent), H. rufipes (3.46percent), R. follis (0.08percent) together with Haemaphysalis silacea (0.02percent) were sporadic infestation. Haemaphysalis elliptica (0.04percent) was only found on the vegetation. No H. rufipes was collected in the thicket vegetation. Agro-ecological zones differ significantly (P<0.05) in tick species and distribution. Significantly more R. (B.) decoloratus were collected in thicket during summer compared to thornveld and dry grassland regions (P<0.05). Free-living ticks were widely distributed across different seasons but with of R. (B.) decoloratus larvae were significantly higher (P<0.05) in thornveld region during the spring season. In conclusion, agro-ecological differences and seasonal variations had an influence on ticks abundance and distribution. From this study, it is of interest to note the absence of R. (B.) microplus, whose trend to invade new areas previously colonised by R. (B.) decoloratus has been observed in Africa. In conclusion, agro-ecological differences and seasonal variations had an influence on ticks abundance and distribution. The aforementioned absence of R. (B.) microplus in this study highlights the importance of further research as this tick invades new areas previously colonised by R. (B.) decoloratus in the continent.
- Full Text:
- Date Issued: 2017
- Authors: Yawa, Mandla
- Date: 2017
- Subjects: Ticks Boophilus microplus
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/9067 , vital:34224
- Description: This study was conducted to determine species composition and seasonal population dynamics of free-living and engorged cattle ticks in three agro-ecological zones (Bedford dry grassland, Kowie thicket and Bisho thornveld) of the Eastern Cape Province, South Africa. The occurrence of ticks on cattle was studied monthly from April 2016 and March 2017. Tick collections were performed on 360 selected cattle in each agro-ecological zones. During tick collections, 10 randomly selected cattle were sampled at each locality and more attention was given to African blue tick and the presence of Asian blue tick. A half body tick collection was performed from cattle and visible ticks were collected by means of fine-forceps after the animals had been restrained. Body regions that were examined includes the ear, head, neck, chest, abdomen, flank, front and hind leg and feet, tail, and peri-anal region. At each locality, six replicate drags of 100 m, approximately 50 m apart, were performed to collect ticks from vegetation. All ticks collected per cattle and from vegetation were stored in labelled sample tubes containing 70percent ethanol, subsequently counted and identified to genus and species level using a standard stereomicroscope. A total of 31425 ticks belonging to ten species were collected during the study. Rhipicephalus (Boophilus) decoloratus, was the most observed tick species (32.50percent), all other ticks like R. evertsi evertsi (18.84percent), R. appendiculatus (17.26percent), A. hebraeum (16.27percent), R. simus (7.69percent) were also commonly collected. Ixodes pilosus (3.84percent), H. rufipes (3.46percent), R. follis (0.08percent) together with Haemaphysalis silacea (0.02percent) were sporadic infestation. Haemaphysalis elliptica (0.04percent) was only found on the vegetation. No H. rufipes was collected in the thicket vegetation. Agro-ecological zones differ significantly (P<0.05) in tick species and distribution. Significantly more R. (B.) decoloratus were collected in thicket during summer compared to thornveld and dry grassland regions (P<0.05). Free-living ticks were widely distributed across different seasons but with of R. (B.) decoloratus larvae were significantly higher (P<0.05) in thornveld region during the spring season. In conclusion, agro-ecological differences and seasonal variations had an influence on ticks abundance and distribution. From this study, it is of interest to note the absence of R. (B.) microplus, whose trend to invade new areas previously colonised by R. (B.) decoloratus has been observed in Africa. In conclusion, agro-ecological differences and seasonal variations had an influence on ticks abundance and distribution. The aforementioned absence of R. (B.) microplus in this study highlights the importance of further research as this tick invades new areas previously colonised by R. (B.) decoloratus in the continent.
- Full Text:
- Date Issued: 2017
The effect of quantitative feed restriction on growth performance, carcass characteristics and selected meat quality parameters in broiler chickens
- Authors: Velele, Siphelo
- Date: 2017
- Subjects: Broilers (Chickens) Poultry -- Breeding Meat -- Quality
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/8888 , vital:33907
- Description: The study investigated the effects of quantitative feed restriction on growth performance, carcass characteristics, internal organs, breast muscle pH and meat colour in broiler chickens. A total of 90, un-sexed, day-old broiler chicks were procured from an accredited supplier and were used for the current trial. For the first 14 days, all birds were brooded in one house and subjected to a uniform day-to-day management. Water was supplied throughout the trial, whereas, feed was only supplied ad libitum between days 1-14 and 29-35 days in feed restricted birds. Feed phases included starter (1-14 days), grower (15-28) and finisher (29-35), respectively. On day 15, birds were randomly allocated to three treatments; each treatment was replicated three times with 10 birds per replicate. The first treatment (T1) group, which acted as control group, was ad libitum feeding for the whole trial duration (1-35 days). For treatments 2 (T2) and 3(T3), 85 percent and 70 percent of the Cobb 500 broiler feed intake standards were applied for a period of 14 days (days 15-28), respectively. Average daily feed intake (ADFI) was determined daily and then average body weight (ABW) was determined weekly. Feed conversion ratio and average daily weight gain was thus computed for each experimental unit (replicate). On day 35, broiler birds were electrically stunned and slaughtered by a sharp knife and then hung for complete bleeding. Data collected included slaughter, carcass, breast, thigh, wing, drumstick, feet, head, spleen, heart, gizzard, intestines (small & large) and liver weights. Breast muscle was further used for the determination of colour (L*, b* and a*), initial (pHi) and ultimate breast pH (pHu) measurements. Although, quantitative feed restriction (T2 & T3) significantly affected growth performance in weeks 3 and 4, birds submitted to T2 performed similar (P>0.05) to control in the final stage, whilst birds in T3 was the poorest (P<0.05). Furthermore, birds subjected to T2 showed no differences of edible carcass portions to the control, however, T3 group showed lower (P<0.05) slaughter and wing weights. Breast ultimate pH values of birds under T2 were similar to control, but birds in T3 had significantly higher (P<0.05) values. Birds under T2 had less (P<0.05) reddish breast meat colour than control at 45 minutes. At 24 hours post-mortem, birds in T3 had lower (P<0.05) lightness values and birds submitted to T2 had higher (P<0.05) yellowness values. Liver weight was significantly lower in birds under T3 and higher (P<0.05) gizzard weights were found in birds subjected to T2. Birds under T2 performed similar to control and were able to compensate for the weight loss when high plane of feed was re-introduced. It can be concluded from the results that restricting 15 percent of feed from Cobb 500 standard feed intake had moderately affected broiler performance. Moreover, restricting 15percent of feed significantly reduced feed intake in broiler chicken.
- Full Text:
- Date Issued: 2017
- Authors: Velele, Siphelo
- Date: 2017
- Subjects: Broilers (Chickens) Poultry -- Breeding Meat -- Quality
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/8888 , vital:33907
- Description: The study investigated the effects of quantitative feed restriction on growth performance, carcass characteristics, internal organs, breast muscle pH and meat colour in broiler chickens. A total of 90, un-sexed, day-old broiler chicks were procured from an accredited supplier and were used for the current trial. For the first 14 days, all birds were brooded in one house and subjected to a uniform day-to-day management. Water was supplied throughout the trial, whereas, feed was only supplied ad libitum between days 1-14 and 29-35 days in feed restricted birds. Feed phases included starter (1-14 days), grower (15-28) and finisher (29-35), respectively. On day 15, birds were randomly allocated to three treatments; each treatment was replicated three times with 10 birds per replicate. The first treatment (T1) group, which acted as control group, was ad libitum feeding for the whole trial duration (1-35 days). For treatments 2 (T2) and 3(T3), 85 percent and 70 percent of the Cobb 500 broiler feed intake standards were applied for a period of 14 days (days 15-28), respectively. Average daily feed intake (ADFI) was determined daily and then average body weight (ABW) was determined weekly. Feed conversion ratio and average daily weight gain was thus computed for each experimental unit (replicate). On day 35, broiler birds were electrically stunned and slaughtered by a sharp knife and then hung for complete bleeding. Data collected included slaughter, carcass, breast, thigh, wing, drumstick, feet, head, spleen, heart, gizzard, intestines (small & large) and liver weights. Breast muscle was further used for the determination of colour (L*, b* and a*), initial (pHi) and ultimate breast pH (pHu) measurements. Although, quantitative feed restriction (T2 & T3) significantly affected growth performance in weeks 3 and 4, birds submitted to T2 performed similar (P>0.05) to control in the final stage, whilst birds in T3 was the poorest (P<0.05). Furthermore, birds subjected to T2 showed no differences of edible carcass portions to the control, however, T3 group showed lower (P<0.05) slaughter and wing weights. Breast ultimate pH values of birds under T2 were similar to control, but birds in T3 had significantly higher (P<0.05) values. Birds under T2 had less (P<0.05) reddish breast meat colour than control at 45 minutes. At 24 hours post-mortem, birds in T3 had lower (P<0.05) lightness values and birds submitted to T2 had higher (P<0.05) yellowness values. Liver weight was significantly lower in birds under T3 and higher (P<0.05) gizzard weights were found in birds subjected to T2. Birds under T2 performed similar to control and were able to compensate for the weight loss when high plane of feed was re-introduced. It can be concluded from the results that restricting 15 percent of feed from Cobb 500 standard feed intake had moderately affected broiler performance. Moreover, restricting 15percent of feed significantly reduced feed intake in broiler chicken.
- Full Text:
- Date Issued: 2017
The use of behaviour, saliva cortisol and faecal glucocorticoid levels as stress indicators in beef cows
- Authors: Dzviti, Melody
- Date: 2017
- Subjects: Veterinary physiology Beef cattle -- Behavior Glucocorticoids
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/8165 , vital:31634
- Description: The study evaluated the use of saliva as a minimally invasive technique for cortisol level determination. This was done by assessing the relationship between serum and saliva cortisol based on short sampling intervals. In addition, the study also determined the effect of successive handling on faecal glucocorticoid metabolites (FGM), rectal temperature (RT), avoidance distance (AD), chute and crush scores as well as urination, defaecation and vocalization in multiparous cows. For trial one six non-pregnant, clinically healthy Nguni cows were used. They were administered with standardised doses of adrenocorticotropic hormone on treatment days and saline was used as a placebo on alternative days. Simultaneously, blood and saliva samples were collected to quantify cortisol levels. No relationship (r = 0.0131, P>0.05) was observed between serum and saliva cortisol. However, results showed that there was adrenal stimulation in saliva coupled by significant (P<0.05) effects of sampling intervals. For the second study 21 non-pregnant and clinically healthy Nguni cows of one herd were used for the trial. They were divided into parity groups and their weights were used as a covariate in data analysis. For five consecutive weeks, fresh faecal samples were extracted and rectal temperatures measured. In addition, fear of approaching humans was assessed by AD scores and behaviour scores in the crush chute, as well as vocalisation, urination and defaecation were observed. The effect of successive handling significantly (P<0.05) affected the physiological parameters, namely FGM and RT. Contrariwise, Chi-square tests revealed that parities had a significant (χ2 < 0.05) influence on AD and crush scores as well as on the occurrence of excretory behaviour. It was concluded that saliva cortisol can be used as a stress indicator in beef cows. However, plasma cortisol can be significantly volatile thereby becoming less sensitive to changes across time. This can cause a lack of relationship between plasma and saliva cortisol. On the other hand, repetitive handling of beef cows has an influence on faecal stress levels. In addition, beef cows of differing parities behave differently to handling stress.
- Full Text:
- Date Issued: 2017
- Authors: Dzviti, Melody
- Date: 2017
- Subjects: Veterinary physiology Beef cattle -- Behavior Glucocorticoids
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/8165 , vital:31634
- Description: The study evaluated the use of saliva as a minimally invasive technique for cortisol level determination. This was done by assessing the relationship between serum and saliva cortisol based on short sampling intervals. In addition, the study also determined the effect of successive handling on faecal glucocorticoid metabolites (FGM), rectal temperature (RT), avoidance distance (AD), chute and crush scores as well as urination, defaecation and vocalization in multiparous cows. For trial one six non-pregnant, clinically healthy Nguni cows were used. They were administered with standardised doses of adrenocorticotropic hormone on treatment days and saline was used as a placebo on alternative days. Simultaneously, blood and saliva samples were collected to quantify cortisol levels. No relationship (r = 0.0131, P>0.05) was observed between serum and saliva cortisol. However, results showed that there was adrenal stimulation in saliva coupled by significant (P<0.05) effects of sampling intervals. For the second study 21 non-pregnant and clinically healthy Nguni cows of one herd were used for the trial. They were divided into parity groups and their weights were used as a covariate in data analysis. For five consecutive weeks, fresh faecal samples were extracted and rectal temperatures measured. In addition, fear of approaching humans was assessed by AD scores and behaviour scores in the crush chute, as well as vocalisation, urination and defaecation were observed. The effect of successive handling significantly (P<0.05) affected the physiological parameters, namely FGM and RT. Contrariwise, Chi-square tests revealed that parities had a significant (χ2 < 0.05) influence on AD and crush scores as well as on the occurrence of excretory behaviour. It was concluded that saliva cortisol can be used as a stress indicator in beef cows. However, plasma cortisol can be significantly volatile thereby becoming less sensitive to changes across time. This can cause a lack of relationship between plasma and saliva cortisol. On the other hand, repetitive handling of beef cows has an influence on faecal stress levels. In addition, beef cows of differing parities behave differently to handling stress.
- Full Text:
- Date Issued: 2017
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