Application of assisted reproduction technologies on the indigenous Nguni cows and heifers
- Authors: Maqhashu, Ayanda
- Date: 2013
- Language: English
- Type: Thesis , Masters , MSc Agric (Animal Science)
- Identifier: vital:11828 , http://hdl.handle.net/10353/d1016096
- Description: The aims of the study were to compare superovulatory (SO) response rate and embryo quality recovered; consequently, correlate sperm motility with fertilization rate on superovulated stud Nguni cows and heifers. Furthermore, compare oestrous synchronization response and pregnancy rate of three breed type cows (Brahman, Bonsmara and Nguni) of different body condition scores following timed artificial insemination in KwaZulu-Natal (KZN) and Limpopo provinces. Nguni stud cows (n= 15) and heifers (n= 10) aged 4-6 and 2-3 years were used as embryo donors. Superovulation of donors involved insertion of a controlled internal drug release device (CIDR) and two injections of FSH daily 12 hours apart for 4 days on a decreasing dosage. Fresh Nguni semen was collected from proven Nguni stud bulls and assessed by computer aided sperm analysis (CASA) before artificial insemination (AI). The doses of AI were prepared and conducted twice, 12 hours apart on synchronized and superovulated Nguni cows or heifers. Embryos were flushed 7 days after AI using a non-surgical technique. Embryos were immediately evaluated under stereo microscope and classified according IETS standard codes (C1, C1- and C2). All transferrable embryos were vitrified. Two pilot study sites were chosen in Eastern Cape – Great kei; (n= 5) and Limpopo - Vuvha; (n=5) provinces for Embryo transfer. Each recipient cow was implanted with one frozen-thawed embryo. For oestrous synchronization, cows (Braman, Bonsmara and Nguni type) were selected in different villages, regardless of parity, age, breed and body weight following pregnancy diagnosis. Cows were grouped according to breed type and body condition scores (BCS) on a scale of 1-5. Group 1 had BCS of ≤ 2.5 in KwaZulu-Natal (n=81) and Limpopo n=71), Group 2 had BCS of ≥ 3 in KwaZulu-Natal (n=79) and Limpopo (n=100) cows. Cows were synchronized by inserting the controlled intravaginal drug release (CIDR) and removed on Day 8, followed by administration of prostaglandin. The white heat mount detectors (HMD) were placed on the individual cow’s tail head as an indicator for oestrous response if colour changed to red and inseminated twice at 12 hours interval. Pregnancy diagnosis was performed by an ultra sound scanner and rectal palpation 90 days after TAI in embryos and semen recipient cows. There was no significant difference on the superovulatory response rate between Nguni cows (40%) and heifers (40%). There was a significant difference on the ovary reaction (number of corpus luteum) of cows (11.33±1.41) and heifers (4.00±0.57). There were no significant differences observed on the embryo quality between Nguni cows (2.5±1.00 and 1.25±0.59) and heifers (0.83±0.41 and 1.00±0.36) for excellent (C1) and good (C1-). However, cows had more numbers of unfertilized ova (5.5±1.05 and 1.75±0.47) and degenerate embryos (3.66±1.00 and 1.25±0.39) than heifers. Village cows responded to oestrous synchronization successfully in KZN (100%) and Limpopo (99%) regardless of body conditions and breed type. The lowest pregnancy rate was recorded in Brahman and Bonsmara type cows with BCS of ≤ 2.5 regardless of Province. Interestingly, Nguni type cows with same body condition of ≤ 2.5 had higher average pregnancy rate of 59.5% in Limpopo and 53.5% in KZN. However, cows with BCS of ≥3 had better pregnancy rate regardless of cow breed type, and province. In conclusion, only 40% of both Nguni cows and heifers responded to superovulation. However, Nguni cows had better ovaries reaction compared to heifers. The quality of embryos recovered was similar for both Nguni cows and heifers. Moreover, there was a positive correlation between total sperm motility and fertilization rate bull 1 (93.7%) inseminated the cows (67.5%) and bull 2 (83.5%) inseminated the heifers (53.5%). Higher pregnancy rate (60%) was recorded in Limpopo compared to Eastern Cape (0%). Interestingly, more than 99% of village cows responded to synchronization and inseminated with frozen-thawed semen successfully. Village Nguni type cows were not affected by body condition scoring as they had higher and similar pregnancy rate as those that had body condition of ≥ 3. It is suggested that it is not advisable to breed synchronized Brahman and Bonsmara type cows with the body condition of ≤ 2.5 except in Nguni cow type as more than 57% average pregnancy rate was achieved.
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- Date Issued: 2013
Effect of agricultural extension services on beneficiaries of the Nguni cattle project: the case of Ncera and Kwezana villages, Eastern Cape Province
- Authors: Gwala, Lindokuhle
- Date: 2013
- Subjects: Agricultural assistance -- South Africa -- Eastern Cape , Agricultural extension work -- South Africa -- Eastern Cape , Agriculture, Cooperative -- South Africa -- Eastern Cape , Farmers -- Education -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc Agric (Agricultural Economics)
- Identifier: vital:11219 , http://hdl.handle.net/10353/d1019814 , Agricultural assistance -- South Africa -- Eastern Cape , Agricultural extension work -- South Africa -- Eastern Cape , Agriculture, Cooperative -- South Africa -- Eastern Cape , Farmers -- Education -- South Africa -- Eastern Cape
- Description: The study was conducted to determine the effect of agricultural extension services on beneficiaries of the Nguni Cattle project in Ncera and Kwezana villages, both in Nkonkobe local Municipality of the Eastern Cape Province. The objectives of this study were to determine the quality of extension services offered to the beneficiaries of the Nguni cattle project, relationship between extension officers and beneficiaries of the project, lastly was to determine communication strategies used by extension officers to communicate with the project beneficiaries. A total of 73 Nguni cattle project beneficiaries were interviewed. Semi- structured questionnaires were administered to the beneficiaries of the project who were willing to participate in the study. Xhosa speaking enumerators assisted in data collection. Focus group discussions were later carried out in both villages to determine effect of agricultural extension services on socio-economic status of the beneficiaries. The focus groups were divided into three groups of different ages and gender. The majority of the beneficiaries in the project were males (62.2 % Ncera and 75% Kwezana). There was an association between gender and extension services. Farmers depended on different sources of income. Old age pension and animal sales being the main contributing sources and also having a significant difference. The results of the study further revealed that the beneficiaries were faced with cattle production challenges, lack of extension support services being the main challenge. The results of the study indicated that the majority of beneficiaries had no access to extension services. Only 37.8% and 32.1 % at Ncera and Kwezana respectively reported that they had access to extension services. Although the latter is the case it was further explained by the beneficiaries that extension services offered to them were of poor quality due to poor communication strategies used to provide these services. Beneficiaries also reported the relationship between them and extension officers’ as poor. It was concluded that extension services had an effect on beneficiaries of the Nguni cattle project, largely based on the constraints they face on the project and limited access to extension services.
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- Date Issued: 2013
Effect of dietary inclusion of Moringa oleifera Lam leaf meal on feed conversion efficiency, meat quality, fatty acid composition, shelf life and consumer health-related perceptions of pork
- Authors: Mukumbo, Felicitas Esnart
- Date: 2013
- Subjects: Moringa oleifera , Meat -- Quality , Feed utilization efficiency
- Language: English
- Type: Thesis , Masters , MSc Agric (Animal Science)
- Identifier: vital:11827 , http://hdl.handle.net/10353/d1016071 , Moringa oleifera , Meat -- Quality , Feed utilization efficiency
- Description: The objective of the study was to determine the feed conversion efficiency (FCE), carcass characteristics, physico-chemical quality, fatty acid (FA) composition and shelf life of pork from pigs fed diets containing either 0% (T1), 2.5% (T2), 5% (T3) or 7.5% (T4) Moringa oleifera leaf meal (MOLM). Consumer health-related perceptions on pork and fatty acids were also investigated. Twenty four crossbred Large White x Landrace pigs of both sexes at 18 weeks of age and initially weighing 71.6 kg on average were housed individually and had ad libitum access to one of the four dietary treatments for a period of six weeks. Average daily feed intake (ADFI) and average daily gain (ADG) and feed conversion ratios (FCR) were calculated as an indication of FCE and pigs were slaughtered at an average live weight of 99.6 kg. Carcass traits such as back fat thickness (BFT), carcass temperatures and pH readings taken 45 minutes and 24 hours (pH45 and pHu) post mortem were recorded. M. longissimus thoracis et lumborum (LTL) samples were taken from each carcass for the determination of lightness (L*), redness (a*), yellowness (b*), thawing loss percentage (TL%), cooking loss percentage (CL%), Warner Bratzler Shear Force (WBSF), shelf life and FA composition. Furthermore a survey was conducted amongst 80 University of Fort Hare Students to determine their health-related perceptions on pork and fatty acids as well as their pork consumption frequency using questionnaires. The FCE of pigs fed on 0-5% MOLM (T1, T2 and T3) diets did not differ significantly, but the FCE of pigs fed 7.5% MOLM (T4) was significantly (p<0.05) reduced. No significant relationship was reported between inclusion of MOLM carcass characteristics and physico-chemical pork quality. There was however a significant improvement (p<0.05) in the shelf life of the pork from MOLM fed pigs in terms of colour and odour during 10 days of refrigerated storage (at 3±1°C). There was a significant (p<0.05) reduction in the total intramuscular fat (IMF) content and the saturated fatty acid (SFA) C18:0 (stearic acid) content and an overall non-significant (p>0.05) increase in the poly unsaturated fatty acid (PUFA) content of pork from MOLM fed pigs. The possible reason for this is that the feeding of MOLM was commenced when the pigs were at an advanced age and weight. The survey revealed that the majority of interviewed students consume fresh (39.7%) and processed (32.4%) pork two to three times a week, perceive pork to be generally healthy (70%) and the second most healthy meat type (39.5%). While the majority (55.4%) were aware of the health implications of FAs they did not know which FA classes pose more of a health risk (51.3%). In conclusion2.5% and 5% of MOLM in finisher pig feed did not negatively affect FCE, carcass characteristics or physico-chemical meat quality; significantly improved pork shelf life and reduced total IMF and SFA content of pork but 7.5% MOLM negatively affected FCE; and the majority of students perceive pork to be the second most healthy type of meat and are generally aware of the health implications of FAs.
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- Date Issued: 2013
Incidence of Staphylococcus species in bovine milk: their antimicrobial sensitivity in selected antibiotics and Usnea barbata lichen extracts
- Authors: Idamokoro, Emrobowansan Monday
- Date: 2013
- Subjects: Staphylococcus , Microbial sensitivity tests , Traditional medicine , Lichens -- Analysis , Mastitis , Antibiotics in agriculture
- Language: English
- Type: Thesis , Masters , MSc Agric (Animal Science)
- Identifier: vital:11829 , http://hdl.handle.net/10353/d1016193 , Staphylococcus , Microbial sensitivity tests , Traditional medicine , Lichens -- Analysis , Mastitis , Antibiotics in agriculture
- Description: This study was done in order to assess the incidence of Staphylococcus species from milk of cows with subclinical mastitis and their antimicrobial sensitivity in some selected antibiotics and Usnea barbata lichen extracts. The study was conducted in two different commercial dairy farms. Staphylococcus species isolates were identified using several biochemical tests which included Gram’s staining test, catalase test and oxidase test. A commercial API® staph kit (bioMerieux, France) was used to confirm the bacterial organisms to their species level. The antimicrobial sensitivity of individual species was determined according to the Clinical Laboratory Standard Institute (CLSI) for the selected antibiotics. Agar well diffusion method and the broth micro-dilution technique were used to determine the sensitivity of Staphylococcus species in U. barbata extracts. A total of 467 milk samples were screened for bacterial identification from the two farms. Fifteen different Staphylococcus species isolates were identified from all milk samples that were examined. The most frequently isolated species included Staphylococcus xylosus (54.34%), Staphylococcus hominis (24.78%), Staphylococcus aureus (16.38%), Staphylococcus saprophyticus (16.12%) and Staphylococcus haemolyticus (11.63%). Most Staphylococcus species were resistant to Penicillin (75.35%), Nalidixic acid (72.55%) and Ampicillin (63%). Furthermore, the bacterial sensitivity evaluation of U. barbata lichen extracted with methanol and ethyl-acetate against selected Staphylococcus species isolates showed 92.31% and 53.85% susceptibility, respectively. The minimum inhibitory concentration (MIC) of the methanol and ethyl-acetate extracts ranged between 0.0390 to 10 mg/ml. There was a relatively high incidence of Staphylococcus species identified in milk of cows with subclinical mastitis from both farms. Conversely, Staphylococcus species isolates were resistant to antibiotics (mostly penicillin and ampicillin) commonly used in the farms. Furthermore, the study investigated the antimicrobial sensitivity of U. barbata extract in-vitro which may validate its use in traditional medicine for treatment of cows with mastitis.
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- Date Issued: 2013
Perceptions on ante-mortem welfare, quantitation of pain and pregnancy biomarkers, muscular fibre architecture and quality of Dohne Merino offal
- Authors: Fayemi, Peter Olutope
- Date: 2013
- Subjects: Merino sheep , Slaughtering and slaughter-houses -- By-products , Biochemical markers , Meat -- Quality , Consumers' preferences , Cooking (Variety meats) , Livestock -- Transportation
- Language: English
- Type: Thesis , Doctoral , PhD (Animal Science)
- Identifier: vital:11824 , http://hdl.handle.net/10353/d1007573 , Merino sheep , Slaughtering and slaughter-houses -- By-products , Biochemical markers , Meat -- Quality , Consumers' preferences , Cooking (Variety meats) , Livestock -- Transportation
- Description: Sheep farming is practiced extensively in South Africa for its significant contributions to the livestock, wool and meat industries. The sheep farming sector in the country has approximately 13,800 farmers with commercial and communal sheep farmers making up 58 percent and 42 percent of the entire work force (Directorate of Agricultural Information Services, 2008). An estimate of 28.8 million sheep and flock size ranging between ≤ 50 and ≥ 1800 exist in various South African provinces. Although the national herd size is unevenly distributed provincially most of the herds are found in the Eastern Cape (30 percent) followed by the Northern Cape (25 percent), Free State (20 percent) and the Western Cape (11 percent) respectively (Agriculture, Forestry and Fisheries, 2011). Over twenty indigenous and locally developed sheep breeds are managed where about 69 percent of the land area is available for their grazing nation-wide (Campher et al., 1998; Palmer and Ainslie, 2006). Common among the indigenous breeds are the Afrikaner, Blackhead Persian, Blackhead Speckled Persian, Blinkhaar Ronderib, Damara, Karakul, Namaqua Afrikaner, Pedi, Redhead Persian, Redhead Speckled, Swazi and Zulu. The locally developed breeds include Dorper, Van Rooy and Merinos. The local breeds developed from Merinos consist of the Afrino, Dormer, Dohne Merino and South African mutton Merino (Hammond, 2000; Pranisha, 2004; Hinton, 2006; Sorma et al., 2012). All these sheep breeds are best suited for providing by-products such as wool, meat, hide, milk or a combination of products (Dave and Meadowcroft, 1996; Jensen, 2009). The indigenous and locally developed sheep were bred to meet the growing demand for its by-products (Peters et al., 2010). Expectedly, sheep farmers therefore, make use of the products from these sheep as a means of livelihood and sustenance of a viable local society (Cloete and Olivier, 2010).
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- Date Issued: 2013
Some performance characteristics of wooled sheep in the sweet and sourveld communal rangelands of Eastern Cape Province, South Africa
- Authors: Mvinjelwa, Sivuyile Alex
- Date: 2013
- Subjects: Wool industry -- South Africa -- Eastern Cape , Sheep -- South Africa -- Eastern Cape , Wool -- South Africa -- Eastern Cape , Veld -- South Africa -- Eastern Cape , Communal rangelands -- South Africa , Grazing -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc Agric (Animal Science)
- Identifier: vital:11825 , http://hdl.handle.net/10353/d1015592 , Wool industry -- South Africa -- Eastern Cape , Sheep -- South Africa -- Eastern Cape , Wool -- South Africa -- Eastern Cape , Veld -- South Africa -- Eastern Cape , Communal rangelands -- South Africa , Grazing -- South Africa -- Eastern Cape
- Description: The objective of the study was to determine sheep growth performance, fleece weights and wool quality characteristics in the sweet and sourveld communal rangelands of the Eastern Cape Province of South Africa. Data were obtained in 80 ewes aged two to three years, selected in four ecologically different communities. Two communities (Roxeni and Tyabane) were in a sweetveld area and the other two communities (Luzi I and Luzi II) were in a sourveld area. The ewes from Tyabane were nondescript, whereas ewes from Roxeni, Luzi I and Luzi II were crossbred between Merinos and non-descript sheep. Selected ewes were monitored for live weight, body condition scoring and fleece weight during shearing. Wool samples were collected from each ewe and were analysed for fibre diameter, comfort factor, clean yield, wool crimps, staple length, staple strength and the Position of break. Sheep from Roxeni were heavier (P <0.05) (39.9 ± 1.1kg) than sheep from Tyabane (29.8 ± 1.1kg). Sheep from Tyabane had the lightest (P <0.05) fleece weight (1.0 ± 1.1kg) and sheep from Roxeni had the heaviest (3.1 ± 1.1kg). There were no significant differences (P ≥0.05) between body condition scores of sheep in Roxeni and the Luzi communities. The live weights of the two-year old ewes were similar (P ≥0.05) to the three-year old ewes’ live weight. However, the three year old ewes had higher (P <0.05) body condition scores than the two year old ewes. Two year old ewes also had lower (P <0.05) fleece weight than the three years old ewes. Wool from Roxeni, Luzi I and Luzi II had lower fibre diameter (P <0.05) than wool from Tyabane. Roxeni sheep had the highest clean yield (P <0.05) of wool, followed by Luzi I and Luzi II; Tyabane had lowest clean yield percentage of wool. Wool from sheep grazing on sweetveld had a higher (P <0.05) clean yield percentage (74.3 ± 0.7%) than those grazing on sourveld (71.7 ± 0.7%). There were higher staple mid-breaks (69.5 ± 5.2) and lower base-breaks (30.5 ± 5.2) in sweetveld (P <0.05) than in sourveld, 7.5 ± 5.2 and 92.5 ± 5.2 for mid-breaks and lower base-breaks, respectively. It was concluded that sheep raised in the sweetveld and bred with purebred Merino rams had higher performance than the ones reared in the sourveld. The young upgraded ewes had a higher wool quality than the older ewes raised on communal rangelands.
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- Date Issued: 2013
Time budgets, blood urea content, total protein and body condition scores as adaptive responses to seasonal dynamics by breeding Nguni cows and heifers reared on a sweetveld
- Authors: Mapfumo, Lizwell
- Date: 2013
- Subjects: Nguni cattle -- South Africa , Cattle -- Feed utilization efficiency -- South Africa , Beef -- Quality -- South Africa , Slaughtering and slaughter-houses -- South Africa , Cattle -- Breeding -- South Africa
- Language: English
- Type: Thesis , Masters , MSc Agric (Animal Science)
- Identifier: vital:11826 , http://hdl.handle.net/10353/d1015738 , Nguni cattle -- South Africa , Cattle -- Feed utilization efficiency -- South Africa , Beef -- Quality -- South Africa , Slaughtering and slaughter-houses -- South Africa , Cattle -- Breeding -- South Africa
- Description: The objective of this study was to determine time budgets, blood urea content, total protein and body condition score as adaptive responses to seasonal dynamics by breeding Nguni cows and heifers reared on a sweetveld. Twenty four clinically healthy Nguni cows and heifers were selected for the study. The animals were grouped according to their parities namely: Parity 1 (n=5), Parity 2 (n=5), Parity 3-6 (old cows) (n=9) and in-calf heifers (n=5). Two separate experiments were conducted and running concurrently. In the first experiment time budgets (time spent walking, grazing, lying down, drinking water and browsing) of the cows and heifers were monitored for two consecutive days once a month, while in the second experiment blood samples were collected from the coccygeal vein once a month during weighing. Body condition scores were also determined during weighing times. Heifers maintained significantly (p0.05) of both blood urea nitrogen (BUN) (7.5±0.39 mmol/l) and TP (80.7±1.19 g/l) in November. All the animals had the least BUN levels in January (1.93±0.18 mmol/l) while the highest total protein (TP) (82.1±1.08 g/l) was recorded in February. All the animals showed distinct (p0.05) in most behavioural attributes in the cool-wet season. Heifers spent significantly (p0.05) body condition scores, amount of time spent browsing and drinking water. On the other hand, heifers (14.7±1.50%) and first parity cows (13.9±1.50%) traded-off most (p<0,05) of their time walking during the cool-dry season. Similarly heifers spent more time browsing (6.4±0.62%) forage plants (p<0.05) than all the cows during the cool-wet season. Grazing (r = 0.17) and standing (r = -0.18) were correlated (p<0.05) with all the behavioural attributes measured in this environment. It was concluded that seasonal dynamics in trade-off behaviour through time budgeting, maintenance of BUN and TP were necessary for the Nguni cows and heifers to maintain their body condition scores within a narrow range throughout the four seasons.
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- Date Issued: 2013