Indigenous forests level of deforestation, forest dependency and factors determining willingness to participate in indigenous forest conservation: evidence from resettled farmers of Shamva, Zimbabwe
- Authors: Chivheya, Renias V
- Date: 2016
- Subjects: Forest conservation -- Zimbabwe Forests and forestry -- Economic aspects -- Zimbabwe Deforestation -- Zimbabwe
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10353/2370 , vital:27786
- Description: This study first explored the rate of forest deforestation in Shamva resettlement areas. It then identified and estimated the extent to which these resettled farmers depend on forest for their livelihoods. Evaluation of farmer perceptions on management issues and willingness to participate in indigenous forest conservation and the socio-economic and institutional factors which affect their willingness to participate were also done. Finally the study sought to identify incentives for forests conservation. The study was conducted in Shamva district in Mashonaland Central province. And the respondents were stratified into three groups: A1, A2 and Old resettlement models. The three models differ on how they were implemented and supported which might render them to have different deforestation rates, livelihood strategies and forest dependency. A total of 247 respondents were surveyed, consisting of 98 A1 farmers, 50 A2 farmers and 99 Old resettled farmers. The data was collected using GIS and remote sensing, structured questionnaire interviews and direct observation. The data was analysed using descriptive analysis, KAP analytic framework and binary logistic regression analysis. The land cover/changes results revealed that both deforestation and afforestation are taking place in Shamva resettlement. Woodland and bushland were decreasing, croplands were also decreasing. However woodland dense and grasslands were increasing. Deforestation was found to be as a result of the resettled farmers’ livelihood strategies which were found to be diverse and agriculture being dominant in all models. All the farmers depended on the forest but at varying levels of 19 percent for Old and 14 percent forA1 and 0.02 percent for A2 resettle farmers. 84 percent of the interviewed farmers however, indicated that they are willing to conserve forest with A1 farmers being the highest followed by A2 86 percent and lastly Old resettled farmers at 76.8 percent. Results of the binary regression model revealed that the significant factors which explain willingness to participate in indigenous forest conservation are age, marital status, education, gender, institution, culture and belief, employment and household size. The highest preferred incentive was the provision of free seedlings and the lowest was out grower scheme. The study recommends that GIS and remote sensing should be used to monitor deforestation, off farm projects be encouraged, exotic and indigenous trees be promoted and forest conservation education be promoted in resettlement areas.
- Full Text:
- Date Issued: 2016
- Authors: Chivheya, Renias V
- Date: 2016
- Subjects: Forest conservation -- Zimbabwe Forests and forestry -- Economic aspects -- Zimbabwe Deforestation -- Zimbabwe
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10353/2370 , vital:27786
- Description: This study first explored the rate of forest deforestation in Shamva resettlement areas. It then identified and estimated the extent to which these resettled farmers depend on forest for their livelihoods. Evaluation of farmer perceptions on management issues and willingness to participate in indigenous forest conservation and the socio-economic and institutional factors which affect their willingness to participate were also done. Finally the study sought to identify incentives for forests conservation. The study was conducted in Shamva district in Mashonaland Central province. And the respondents were stratified into three groups: A1, A2 and Old resettlement models. The three models differ on how they were implemented and supported which might render them to have different deforestation rates, livelihood strategies and forest dependency. A total of 247 respondents were surveyed, consisting of 98 A1 farmers, 50 A2 farmers and 99 Old resettled farmers. The data was collected using GIS and remote sensing, structured questionnaire interviews and direct observation. The data was analysed using descriptive analysis, KAP analytic framework and binary logistic regression analysis. The land cover/changes results revealed that both deforestation and afforestation are taking place in Shamva resettlement. Woodland and bushland were decreasing, croplands were also decreasing. However woodland dense and grasslands were increasing. Deforestation was found to be as a result of the resettled farmers’ livelihood strategies which were found to be diverse and agriculture being dominant in all models. All the farmers depended on the forest but at varying levels of 19 percent for Old and 14 percent forA1 and 0.02 percent for A2 resettle farmers. 84 percent of the interviewed farmers however, indicated that they are willing to conserve forest with A1 farmers being the highest followed by A2 86 percent and lastly Old resettled farmers at 76.8 percent. Results of the binary regression model revealed that the significant factors which explain willingness to participate in indigenous forest conservation are age, marital status, education, gender, institution, culture and belief, employment and household size. The highest preferred incentive was the provision of free seedlings and the lowest was out grower scheme. The study recommends that GIS and remote sensing should be used to monitor deforestation, off farm projects be encouraged, exotic and indigenous trees be promoted and forest conservation education be promoted in resettlement areas.
- Full Text:
- Date Issued: 2016
Individualism, collectivism and gender as correlates of the work ethic among the employees in a government department in the Eastern Cape
- Authors: Gwelo, Blessing
- Date: 2016
- Subjects: Individualism Collectivism Work ethic
- Language: English
- Type: Thesis , Masters , Industrial Psychology
- Identifier: http://hdl.handle.net/10353/11608 , vital:39089
- Description: This study investigated the relationship between individualism, collectivism and gender among the employees at the Department of Justice and Constitutional Development situated in East London, South Africa. Individualism, collectivism and gender were used as independent variables in the study while the work ethic was used as the dependent variable. Data was gathered from a random sample of 260 employees in the Department. A questionnaire consisting of three parts was used for data collection. To gather biographical and occupational data, a self-designed 5-item questionnaire was used. The 65 item Multidimensional Work Ethic Profile (MWEP) scale Miller et al. (2002) was used to measure the work ethic. A 16-item individualism/collectivism scale (also known as the Culture Orientation Scale) formulated by Triandis & Gelfand (1998) was used in the research to measure individualism/collectivism. Data analysis was done by means of various statistical techniques, including the Pearson Product Moment Correlation Technique and Multiple Regression Analysis. The results indicated that there is a significant positive correlation between individualism and the work ethic, noting that a positive correlation for individualism implies a negative correlation for collectivism. The results of this study also found males showing to have higher work ethic than females. The results indicated that individualism/collectivism and gender together do not account for a higher proportion of variance in work ethic than any of the two separately. The study, therefore, recommends that managers must do all in their power to promote the growth and development of work ethic in their organisations through encouraging individualism, and through striving to increase the work ethic in females.
- Full Text:
- Date Issued: 2016
- Authors: Gwelo, Blessing
- Date: 2016
- Subjects: Individualism Collectivism Work ethic
- Language: English
- Type: Thesis , Masters , Industrial Psychology
- Identifier: http://hdl.handle.net/10353/11608 , vital:39089
- Description: This study investigated the relationship between individualism, collectivism and gender among the employees at the Department of Justice and Constitutional Development situated in East London, South Africa. Individualism, collectivism and gender were used as independent variables in the study while the work ethic was used as the dependent variable. Data was gathered from a random sample of 260 employees in the Department. A questionnaire consisting of three parts was used for data collection. To gather biographical and occupational data, a self-designed 5-item questionnaire was used. The 65 item Multidimensional Work Ethic Profile (MWEP) scale Miller et al. (2002) was used to measure the work ethic. A 16-item individualism/collectivism scale (also known as the Culture Orientation Scale) formulated by Triandis & Gelfand (1998) was used in the research to measure individualism/collectivism. Data analysis was done by means of various statistical techniques, including the Pearson Product Moment Correlation Technique and Multiple Regression Analysis. The results indicated that there is a significant positive correlation between individualism and the work ethic, noting that a positive correlation for individualism implies a negative correlation for collectivism. The results of this study also found males showing to have higher work ethic than females. The results indicated that individualism/collectivism and gender together do not account for a higher proportion of variance in work ethic than any of the two separately. The study, therefore, recommends that managers must do all in their power to promote the growth and development of work ethic in their organisations through encouraging individualism, and through striving to increase the work ethic in females.
- Full Text:
- Date Issued: 2016
Job Engagement and Coping Strategies as Moderators of the Relationship between Occupational Stress and Burnout among Police Officers in the Eastern Cape, South Africa
- Authors: Dywili, Mtutuzeli
- Date: 2016
- Subjects: Police -- Job stress -- South Africa -- Eastern cape Job satisfaction -- South Africa -- Eastern Cape Employee motivation -- South Africa -- Eastern Cape Work -- Psychological aspects
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10353/7697 , vital:30707
- Description: Police work in South Africa is demanding and stressful. Literature reveals that occupational stress when fully manifest amount to emotional burnout. It was therefore the aim of this study to examine job engagement and coping strategies as moderators of the relationship between occupational stress and burnout among police officers. Using a sample of 377 police officers from the Eastern Cape, South Africa, data was collected using various scales namely; the Effort – Reward Imbalance (ERI) questionnaire (occupational stress), the Maslach Burnout Inventory – General Survey MBI-GS (emotional burnout), the Utrecht Work Engagement Scale (job engagement), and the Coping Orientation to Problems Experienced questionnaire (coping strategies). Correlation tests and regression analysis were ministered on the data to test the research hypotheses. The major finding of the study indicated that job engagement and coping strategies collectively significantly moderate the relationship between occupational stress and emotional burnout. However, job engagement on its own was reported to be an insignificant moderator of the relationship between occupational stress and emotional burnout.
- Full Text:
- Date Issued: 2016
- Authors: Dywili, Mtutuzeli
- Date: 2016
- Subjects: Police -- Job stress -- South Africa -- Eastern cape Job satisfaction -- South Africa -- Eastern Cape Employee motivation -- South Africa -- Eastern Cape Work -- Psychological aspects
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10353/7697 , vital:30707
- Description: Police work in South Africa is demanding and stressful. Literature reveals that occupational stress when fully manifest amount to emotional burnout. It was therefore the aim of this study to examine job engagement and coping strategies as moderators of the relationship between occupational stress and burnout among police officers. Using a sample of 377 police officers from the Eastern Cape, South Africa, data was collected using various scales namely; the Effort – Reward Imbalance (ERI) questionnaire (occupational stress), the Maslach Burnout Inventory – General Survey MBI-GS (emotional burnout), the Utrecht Work Engagement Scale (job engagement), and the Coping Orientation to Problems Experienced questionnaire (coping strategies). Correlation tests and regression analysis were ministered on the data to test the research hypotheses. The major finding of the study indicated that job engagement and coping strategies collectively significantly moderate the relationship between occupational stress and emotional burnout. However, job engagement on its own was reported to be an insignificant moderator of the relationship between occupational stress and emotional burnout.
- Full Text:
- Date Issued: 2016
Learners' views regarding their effective participation in school governance
- Authors: Munikwa, Julius
- Date: 2016
- Subjects: Student participation in administration
- Language: English
- Type: Thesis , Masters , Education
- Identifier: http://hdl.handle.net/10353/16013 , vital:40582
- Description: Effective learner participation for the development of Representative Council of Learners (RCL) governors in school governance (SG) is of great essence. A qualitative study was conducted in order to assess the learners’ views regarding their lived views and experiences in SG. Twenty four (24) RCL and class representatives from three data sites comprising three high schools in East Cape, in the East London District Education participated in the study. Data was gathered through semi-structured interviews, focus group discussions and document analysis from two convenient and one snowball samples, respectively. From each school, a chairperson, a secretary and six focus group discussions (FDGs) members were utilised as participants in order to enrich the study. In doing so, the study made use of Wilcox’s ladder of participation as the lens to unpack the learners’ views regarding their effective participation in school governance. This ladder includes information, consultation, deciding together, acting together and supporting independent community interests. The findings showed that learner participation in both township and former Model C schools is significant. However, a number of pertinent factors that make the voices of learner governors to be stifled emerged. These include lack of capacity building, exclusion from other meetings, dominance of adult governors, lack of collaboration and the fact that learners have to be seen as being respectful and disciplined when in meetings with parents. This makes them to accept their role as a tool for middlemen between the parents, teachers and fellow students. These make the field of effective learner participation uneven, hence making them window dressers and have pseudo contributions in SG. The study therefore suggested that learners should be capacitated to make effective and meaningful contribution in school governance. Learners should work collaboratively with adult governors to enhance effective participation in SG.
- Full Text:
- Date Issued: 2016
- Authors: Munikwa, Julius
- Date: 2016
- Subjects: Student participation in administration
- Language: English
- Type: Thesis , Masters , Education
- Identifier: http://hdl.handle.net/10353/16013 , vital:40582
- Description: Effective learner participation for the development of Representative Council of Learners (RCL) governors in school governance (SG) is of great essence. A qualitative study was conducted in order to assess the learners’ views regarding their lived views and experiences in SG. Twenty four (24) RCL and class representatives from three data sites comprising three high schools in East Cape, in the East London District Education participated in the study. Data was gathered through semi-structured interviews, focus group discussions and document analysis from two convenient and one snowball samples, respectively. From each school, a chairperson, a secretary and six focus group discussions (FDGs) members were utilised as participants in order to enrich the study. In doing so, the study made use of Wilcox’s ladder of participation as the lens to unpack the learners’ views regarding their effective participation in school governance. This ladder includes information, consultation, deciding together, acting together and supporting independent community interests. The findings showed that learner participation in both township and former Model C schools is significant. However, a number of pertinent factors that make the voices of learner governors to be stifled emerged. These include lack of capacity building, exclusion from other meetings, dominance of adult governors, lack of collaboration and the fact that learners have to be seen as being respectful and disciplined when in meetings with parents. This makes them to accept their role as a tool for middlemen between the parents, teachers and fellow students. These make the field of effective learner participation uneven, hence making them window dressers and have pseudo contributions in SG. The study therefore suggested that learners should be capacitated to make effective and meaningful contribution in school governance. Learners should work collaboratively with adult governors to enhance effective participation in SG.
- Full Text:
- Date Issued: 2016
Leisure time and holiday aspirations of black upper middle class in East London
- Authors: Ngozwana, Baselwa
- Date: 2016
- Subjects: Social classes -- South Africa -- Eastern Cape Lifestyles -- South Africa
- Language: English
- Type: Thesis , Masters , MSoc
- Identifier: http://hdl.handle.net/10353/6380 , vital:29600
- Description: This study examined the holiday and leisure aspirations of the new upper black middle class in East London using qualitative approach. The study investigated aspirations of the black middle class on international travels through the lens of Veblen’s (2002) conspicuous consumption theory which entails that black middle class citizens spend in order to assert their status and belongingness to the middle class. The aim of the study was to understand their holiday and leisure aspirations, what the idea is behind or what motivates their international travels, their travel experiences and class affirmations. This exploration included the challenges and frustrations such as Ebola scare, racism, issues with foreign exchange and variations from these international visits. Data was collected through interviews with upper black middle class who are based in the Buffalo City Metro Municipality in East London, Eastern Cape. Findings from this study revealed that consumption patterns of the emerging black middle class to diverge substantially from the other groups, in terms of greater signalling of social status via visible consumption and preoccupation with reducing an historical asset deficit. Various themes emerged as meanings attached to international travelling and leisure by those black middle class international travellers. These are sense of wealth, status, religion, self-esteem, sense of reward, bonding with friends and conspicuous/visible consumption.
- Full Text:
- Date Issued: 2016
- Authors: Ngozwana, Baselwa
- Date: 2016
- Subjects: Social classes -- South Africa -- Eastern Cape Lifestyles -- South Africa
- Language: English
- Type: Thesis , Masters , MSoc
- Identifier: http://hdl.handle.net/10353/6380 , vital:29600
- Description: This study examined the holiday and leisure aspirations of the new upper black middle class in East London using qualitative approach. The study investigated aspirations of the black middle class on international travels through the lens of Veblen’s (2002) conspicuous consumption theory which entails that black middle class citizens spend in order to assert their status and belongingness to the middle class. The aim of the study was to understand their holiday and leisure aspirations, what the idea is behind or what motivates their international travels, their travel experiences and class affirmations. This exploration included the challenges and frustrations such as Ebola scare, racism, issues with foreign exchange and variations from these international visits. Data was collected through interviews with upper black middle class who are based in the Buffalo City Metro Municipality in East London, Eastern Cape. Findings from this study revealed that consumption patterns of the emerging black middle class to diverge substantially from the other groups, in terms of greater signalling of social status via visible consumption and preoccupation with reducing an historical asset deficit. Various themes emerged as meanings attached to international travelling and leisure by those black middle class international travellers. These are sense of wealth, status, religion, self-esteem, sense of reward, bonding with friends and conspicuous/visible consumption.
- Full Text:
- Date Issued: 2016
Management factors influencing lamb survival from birth to weaning in two ecologically different resource-limited communal farming systems
- Authors: Lungu, Nobuhle
- Date: 2016
- Subjects: Lambs Communal rangelands Lambs--Feeding and feeds
- Language: English
- Type: Thesis , Masters , Animal Science
- Identifier: http://hdl.handle.net/10353/11806 , vital:39108
- Description: The objective of the study was to determine the management factors influencing lamb survival from birth to weaning in two ecologically different resource-limited communal farming systems. An annual flock monitoring study in the sourveld and sweetveld regions was preceded by a household check-all-that-apply (CATA). Lamb management practices and constraints faced in raising lambs in the two regions were determined using the check-all-that-apply technique. The results showed that the majority of the households were male headed (75.70percent) and consisted of the elderly (˃50 years, 79.44percent) who were not employed (82.24percent) and had attended primary school (67.29percent). Wool production was ranked the most important reason for keeping sheep in the two regions. The CATA revealed that the management systems between the two regions differed significantly (p˂0.05) in terms of assisting sheep at lambing, attending to twin births and dipping of mature lambs. Farmers‘ age was associated with the type of management performed (p ˂0.05). None of the farmers in the two regions performed navel dipping. Some of the reported constraints in the sweetveld were theft, diarrhoea and drought while predators and cold weather were the major constraints in the sourveld. The CATA identified vaccination and dipping as the major missing practices in both regions. The study showed differences in the use and choice of CATA terms which suggests that a ―one-size-fits-all‖ approach to solving poor management and sheep production constraints is not applicable. In the monitoring study, the results showed that veld type had no significant effect on the birth weights (2.0±0.09 kg) of the lambs. Type of birth had a significant effect (p<0.05) on the birth weights of the lambs. Single born lambs were 0.41kg heavier (p<0.05) than twin lambs. Single born lambs, however, showed an improvement in weights at 90 and 120 days where they had slightly higher weights than single born lambs. Male lambs were significantly (p<0.05) heavier (2.3±0.12 kg) than the female lambs (1.7±0.06 kg) at birth. The lamb birth were highest (P<0.05) in the hot-dry season. Lambs born in the cold-dry season were the lightest (1.63±0.13 kg). Lambs born in the post- rainy and hot-wet season were not significantly different (P<0.05). Weaning weights were not affected by veld type, birth type and sex, but were affected by season of birth. The lambs born in the hot-dry season had the highest weaning weights. The sourveld had a total pre-weaning mortality of 10.6percent while the sweetveld had a total pre-weaning mortality of 14.2percent. Animal housing from both veld types was poorly designed in terms of security against stock theft, drainage, hygiene, overhead shelter from the rain as well as access to feed and water. It was concluded that lamb management practices in the two regions were not the same. Performance of lambs before weaning differed according to veld type which played a role in feed availability during different seasons.
- Full Text:
- Date Issued: 2016
- Authors: Lungu, Nobuhle
- Date: 2016
- Subjects: Lambs Communal rangelands Lambs--Feeding and feeds
- Language: English
- Type: Thesis , Masters , Animal Science
- Identifier: http://hdl.handle.net/10353/11806 , vital:39108
- Description: The objective of the study was to determine the management factors influencing lamb survival from birth to weaning in two ecologically different resource-limited communal farming systems. An annual flock monitoring study in the sourveld and sweetveld regions was preceded by a household check-all-that-apply (CATA). Lamb management practices and constraints faced in raising lambs in the two regions were determined using the check-all-that-apply technique. The results showed that the majority of the households were male headed (75.70percent) and consisted of the elderly (˃50 years, 79.44percent) who were not employed (82.24percent) and had attended primary school (67.29percent). Wool production was ranked the most important reason for keeping sheep in the two regions. The CATA revealed that the management systems between the two regions differed significantly (p˂0.05) in terms of assisting sheep at lambing, attending to twin births and dipping of mature lambs. Farmers‘ age was associated with the type of management performed (p ˂0.05). None of the farmers in the two regions performed navel dipping. Some of the reported constraints in the sweetveld were theft, diarrhoea and drought while predators and cold weather were the major constraints in the sourveld. The CATA identified vaccination and dipping as the major missing practices in both regions. The study showed differences in the use and choice of CATA terms which suggests that a ―one-size-fits-all‖ approach to solving poor management and sheep production constraints is not applicable. In the monitoring study, the results showed that veld type had no significant effect on the birth weights (2.0±0.09 kg) of the lambs. Type of birth had a significant effect (p<0.05) on the birth weights of the lambs. Single born lambs were 0.41kg heavier (p<0.05) than twin lambs. Single born lambs, however, showed an improvement in weights at 90 and 120 days where they had slightly higher weights than single born lambs. Male lambs were significantly (p<0.05) heavier (2.3±0.12 kg) than the female lambs (1.7±0.06 kg) at birth. The lamb birth were highest (P<0.05) in the hot-dry season. Lambs born in the cold-dry season were the lightest (1.63±0.13 kg). Lambs born in the post- rainy and hot-wet season were not significantly different (P<0.05). Weaning weights were not affected by veld type, birth type and sex, but were affected by season of birth. The lambs born in the hot-dry season had the highest weaning weights. The sourveld had a total pre-weaning mortality of 10.6percent while the sweetveld had a total pre-weaning mortality of 14.2percent. Animal housing from both veld types was poorly designed in terms of security against stock theft, drainage, hygiene, overhead shelter from the rain as well as access to feed and water. It was concluded that lamb management practices in the two regions were not the same. Performance of lambs before weaning differed according to veld type which played a role in feed availability during different seasons.
- Full Text:
- Date Issued: 2016
Mechanism and synchronicity of wheat (Triticum aestivum) resistance to leaf rust (Puccinia triticina) and Russian wheat aphid (Duiraphis noxia) SA1
- Authors: Njom, Henry Akum
- Date: 2016
- Subjects: Wheat -- Disease and pest resistance Bacterial diseases of plants Russian wheat aphid
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10353/2700 , vital:28056
- Description: Wheat (Triticum aestivum and T. Durum) is an extremely important agronomic crop produced worldwide. Wheat consumption has doubled in the last 30 years with approximately 600 million tons consumed per annum. According to the International Maize and Wheat Improvement Center, worldwide wheat demand will increase over 40 percent by 2020, while land as well as resources available for the production will decrease significantly if the current trend prevails. The wheat industry is challenged with abiotic and biotic stressors that lead to reduction in crop yields. Increase knowledge of wheat’s biochemical constitution and functional biology is of paramount importance to improve wheat so as to meet with this demand. Pesticides and fungicides are being used to control biotic stress imposed by insect pest and fungi pathogens but these chemicals pose a risk to the environment and human health. To this effect, there is re-evaluation of pesticides currently in use by the Environmental Protection Agency, via mandates of the 1996 Food Quality Protection Act and those with higher perceived risks are banned. Genetic resistance is now a more environmental friendly and effective method of controlling insect pest and rust diseases of wheat than the costly spraying with pesticides and fungicides. Although, resistant cultivars effectively prevent current prevailing pathotypes of leaf rust and biotypes of Russian wheat aphid from attacking wheat, new pathotypes and biotypes of the pathogen/pest may develop and infect resistant cultivars. Therefore, breeders are continually searching for new sources of resistance. Proteomic approaches can be utilised to ascertain target enzymes and proteins from resistant lines that could be utilised to augment the natural tolerance of agronomically favourable varieties of wheat. With this ultimate goal in mind, the aim of this study was to elucidate the mechanism and synchronicity of wheat resistance to leaf rust (Puccinia triticina) and Russian wheat aphid (Duiraphis noxia) SA1. To determine the resistance mechanism of the wheat cultivars to leaf rust infection and Russian wheat aphid infestation, a proteomics approach using two-dimensional gel electrophoresis was used in order to determine the effect of RWA SA1 on the wheat cultivars proteome. Differentially expressed proteins that were up or down regulated (appearing or disappearing) were identified using PDQuestTM Basic 2-DE Gel analysis software. Proteins bands of interest were in-gel trypsin digested as per the protocol described in Schevchenko et al. (2007) and analysed using a Dionex Ultimate 3000 RSLC system coupled to an AB Sciex 6600 TripleTOF mass spectrometer. Protein pilot v5 using Paragon search engine (AB Sciex) was used for comparison of the obtained MS/MS spectra with a custom database containing sequences of Puccinia triticina (Uniprot Swissprot), Triticum aestivum (Uniprot TrEMBL) and Russian wheat aphid (Uniprot TrEMBL) as well as a list of sequences from common contaminating proteins. Proteins with a threshold of ≥99.9 percent confidence were reported. A total of 72 proteins were putatively identified from the 37 protein spots excised originating from either leaf rust or Russian wheat aphid experiments. Sixty-three of these proteins were associated with wheat response to stress imposed by RWA SA1 feeding while 39 were associated with infection by Puccinia triticina. Several enzymes involved in the Calvin cycle, electron transport and ATP synthesis were observed to be differentially regulated suggesting greater metabolic requirements in the wheat plants following aphid infestation and leaf rust infection. Proteins directly associated with photosynthesis were also differentially regulated following RWA SA1 infestation and P.
- Full Text:
- Date Issued: 2016
- Authors: Njom, Henry Akum
- Date: 2016
- Subjects: Wheat -- Disease and pest resistance Bacterial diseases of plants Russian wheat aphid
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10353/2700 , vital:28056
- Description: Wheat (Triticum aestivum and T. Durum) is an extremely important agronomic crop produced worldwide. Wheat consumption has doubled in the last 30 years with approximately 600 million tons consumed per annum. According to the International Maize and Wheat Improvement Center, worldwide wheat demand will increase over 40 percent by 2020, while land as well as resources available for the production will decrease significantly if the current trend prevails. The wheat industry is challenged with abiotic and biotic stressors that lead to reduction in crop yields. Increase knowledge of wheat’s biochemical constitution and functional biology is of paramount importance to improve wheat so as to meet with this demand. Pesticides and fungicides are being used to control biotic stress imposed by insect pest and fungi pathogens but these chemicals pose a risk to the environment and human health. To this effect, there is re-evaluation of pesticides currently in use by the Environmental Protection Agency, via mandates of the 1996 Food Quality Protection Act and those with higher perceived risks are banned. Genetic resistance is now a more environmental friendly and effective method of controlling insect pest and rust diseases of wheat than the costly spraying with pesticides and fungicides. Although, resistant cultivars effectively prevent current prevailing pathotypes of leaf rust and biotypes of Russian wheat aphid from attacking wheat, new pathotypes and biotypes of the pathogen/pest may develop and infect resistant cultivars. Therefore, breeders are continually searching for new sources of resistance. Proteomic approaches can be utilised to ascertain target enzymes and proteins from resistant lines that could be utilised to augment the natural tolerance of agronomically favourable varieties of wheat. With this ultimate goal in mind, the aim of this study was to elucidate the mechanism and synchronicity of wheat resistance to leaf rust (Puccinia triticina) and Russian wheat aphid (Duiraphis noxia) SA1. To determine the resistance mechanism of the wheat cultivars to leaf rust infection and Russian wheat aphid infestation, a proteomics approach using two-dimensional gel electrophoresis was used in order to determine the effect of RWA SA1 on the wheat cultivars proteome. Differentially expressed proteins that were up or down regulated (appearing or disappearing) were identified using PDQuestTM Basic 2-DE Gel analysis software. Proteins bands of interest were in-gel trypsin digested as per the protocol described in Schevchenko et al. (2007) and analysed using a Dionex Ultimate 3000 RSLC system coupled to an AB Sciex 6600 TripleTOF mass spectrometer. Protein pilot v5 using Paragon search engine (AB Sciex) was used for comparison of the obtained MS/MS spectra with a custom database containing sequences of Puccinia triticina (Uniprot Swissprot), Triticum aestivum (Uniprot TrEMBL) and Russian wheat aphid (Uniprot TrEMBL) as well as a list of sequences from common contaminating proteins. Proteins with a threshold of ≥99.9 percent confidence were reported. A total of 72 proteins were putatively identified from the 37 protein spots excised originating from either leaf rust or Russian wheat aphid experiments. Sixty-three of these proteins were associated with wheat response to stress imposed by RWA SA1 feeding while 39 were associated with infection by Puccinia triticina. Several enzymes involved in the Calvin cycle, electron transport and ATP synthesis were observed to be differentially regulated suggesting greater metabolic requirements in the wheat plants following aphid infestation and leaf rust infection. Proteins directly associated with photosynthesis were also differentially regulated following RWA SA1 infestation and P.
- Full Text:
- Date Issued: 2016
Mitigation of the effect of father imprisonment on the family: a study of social work intervention strategies in Raymond Mhlaba local municipality
- Authors: Tshaka, Akhona
- Date: 2016
- Subjects: Social work with single parents Single mothers -- South Africa -- Eastern Cape Single parents -- South Africa -- Eastern Cape Single-parent families -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , Degree
- Identifier: http://hdl.handle.net/10353/4435 , vital:28334
- Description: The South African population is highly populated by single mothered families. Fatherlessness has emerged as one of the greatest social problems, especially as children who grow up in families with absent-fathers suffer lasting damage. The aim of this study was to examine the effect of fathers’ imprisonment on the functioning of the family and social work interventions to mitigate the effect. The study used mixed method research design. Data was collected through the use of questionnaires and in-depth interviews. This study employed three sampling strategies; namely multi-stage sampling and purposive sampling as well as snow-ball sampling. The sample was made up of 65 members of families whose father is in prison or had been to prision and 15 social work officials. Qalitative data was analyised using themes and parrtens that emerged during interviews with participants, quantitative data was analysed using SPSS computer softerwere. Findings revealed that father’s imprisonment has negative impact that causes dysfunctional families. The negative effect is due to the loss of a father figure and a provider of the family. Inequality and stigmatisation among family members were also found to cause dysfunctional families after the father’s imprisonment. Findings from social work officials revealed social service interventions to mollify the family dysfunctions. However, majority of families are provided services through offender re-integration, others lack unawareness regarding social work interventions for families with fathers in prison. Findings further reveal that resources are limited to enhance the provision of adequate and better psychosocial support for affected families in order to alleviate the undesirable impact of father’s imprisonment. There is therefore a compelling need for implementing policies that will enable dysfunctional families with fathers imprison to access adequate psychosocial support services. The study also recommended that, social welfare system should be strengthened by embarking on enlightenment programmes that create awareness about family based interventions for dysfunction families with fathers in prison.
- Full Text:
- Date Issued: 2016
- Authors: Tshaka, Akhona
- Date: 2016
- Subjects: Social work with single parents Single mothers -- South Africa -- Eastern Cape Single parents -- South Africa -- Eastern Cape Single-parent families -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , Degree
- Identifier: http://hdl.handle.net/10353/4435 , vital:28334
- Description: The South African population is highly populated by single mothered families. Fatherlessness has emerged as one of the greatest social problems, especially as children who grow up in families with absent-fathers suffer lasting damage. The aim of this study was to examine the effect of fathers’ imprisonment on the functioning of the family and social work interventions to mitigate the effect. The study used mixed method research design. Data was collected through the use of questionnaires and in-depth interviews. This study employed three sampling strategies; namely multi-stage sampling and purposive sampling as well as snow-ball sampling. The sample was made up of 65 members of families whose father is in prison or had been to prision and 15 social work officials. Qalitative data was analyised using themes and parrtens that emerged during interviews with participants, quantitative data was analysed using SPSS computer softerwere. Findings revealed that father’s imprisonment has negative impact that causes dysfunctional families. The negative effect is due to the loss of a father figure and a provider of the family. Inequality and stigmatisation among family members were also found to cause dysfunctional families after the father’s imprisonment. Findings from social work officials revealed social service interventions to mollify the family dysfunctions. However, majority of families are provided services through offender re-integration, others lack unawareness regarding social work interventions for families with fathers in prison. Findings further reveal that resources are limited to enhance the provision of adequate and better psychosocial support for affected families in order to alleviate the undesirable impact of father’s imprisonment. There is therefore a compelling need for implementing policies that will enable dysfunctional families with fathers imprison to access adequate psychosocial support services. The study also recommended that, social welfare system should be strengthened by embarking on enlightenment programmes that create awareness about family based interventions for dysfunction families with fathers in prison.
- Full Text:
- Date Issued: 2016
Molecular characterization of Mycobacterium bovis isolated from cattle and sputum specimens from humans in the Eastern Cape province, South Africa : public health and economic implication
- Authors: Bhembe, Nolwazi Londiwe
- Date: 2016
- Subjects: Mycobacterium tuberculosis -- South Africa -- Eastern Cape Public health -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10353/13238 , vital:39625
- Description: Tuberculosis (TB) is a disease caused by the members of the Mycobacterium tuberculosis complex (MTBC), which infects both animals and humans. The primary host of Mycobacterium bovis is cattle. It remains a major global health problem. About 2 billion people are thought to be infected with TB and about 1.3 million decease each year from the disease. This study is aimed at determining the prevalence of the disease, characterizing the MTBC isolates and increasing the understanding of the genetic diversity MTBC in the Eastern Cape Province of South Africa, from studying both samples from both humans and cattle. A total of 376 cattle lymph nodes were collected from two commercial abattoirs for investigations. Suspected 6 000 sputum specimens were also collected from humans (cattle farmers). All collected samples were cultured, and isolates were confirmed with a multiplex polymerase chain reaction (PCR) targeting the mpb64 and IS6110 genes. Positive isolates were tested for resistance against anti-TB drugs using the standard Lowenstein Jensen proportion method. Isolates from cattle lymph nodes were further characterized to species level, using the region of deletion 1 (RD1) and oxyR restriction fragment length polymorphism analyses. MIRU-VNTR typing and spoligotyping assays were used to further examine the genetic diversity of all strains. Obtained spoligotype patterns were matched with isolates published in the universal spoligotyping database (SITVIT2). MTBC was detected in 162 (43.09 percent) cattle lymph nodes and 156 sputum specimens. Some isolates 25.9 percent (42/162) from the cattle lymph nodes samples were resistant to rifampicin (RIF) and 98.7 percent (154/156) were resistant to isoniazid (INH) and rifampicin (RIF) (of the human sputum specimens). All cattle lymph nodes isolates exhibited the RD1 gene, and the oxyR analyses classified 96.9 percent of the isolates as Mycobacterium tuberculosis complex and 3.1 percent as Mycobacterium bovis. Twenty seven spoligotype patterns were isolated from cattle lymph nodes, with 10 shared types (SIT) consigned to 5 lineages, including Bov_4-Caprea (1.9 percent), Microti (0.6 percent) and Beijing (17.9 percent) constituting East Asian, Latin-American-Mediterranean (LAM) (3.7 percent), X (4.3 percent), MANU (3.1percent), S (0.6 percent) and 67.9 percent were orphans that were further categorized to their designated lineages (Indo-Oceanic, Euro-American and West-African 2). Ninety one percent of the isolates from humans (farmers) were grouped into 7 clusters by spoligotyping, while the remaining 9 percent was unclustered. SITs were observed in 89.6 percent of the isolates from the Beijing, LAM, X, T and S families. The Beijing family was the most prevalent strain from both human isolates (77.2 percent) and cattle lymph nodes isolates (17.9 percent), and the T (0.6 percent) and Microti (0.6 percent) families were the least detected genotypes in both humans and cattle lymph nodes. In the cattle lymph nodes, spoligotyping showed a higher clustering rate of 82.1 percent, with the lowest (HGDI) = 0.485. The 12 MIRU-VNTR clustering rate was 64.8 percent, showing a higher HGDI of 0.671. However, a combination of both methods showed a decreased clustering rate of 53.7 percent; however these methods resulted in a higher HGDI (0.676). Isolates from human sputum specimens spoligotyping showed a higher clustering rate of 89 percent with an HGDI = 0.822. The 12 MIRU-VNTR showed the lowest clustering rate of 69 percent and an HGDI = 0.940 and the combination of the methods showed a clustering rate of 78 percent and an HGDI = 0.951. The results of this study show the diversity of MTBC strains in the Eastern Cape Province and the low clustering rate indicates continuing transmission in the province. The detection of MTBC strains from slaughtered cattle lymph nodes in abattoirs confirms the risk of providing TB infected meat in markets which has serious implications for the control of TB in South Africa. The continuing spread of MTBC in South Africa threatens both the public and economic health.
- Full Text:
- Date Issued: 2016
- Authors: Bhembe, Nolwazi Londiwe
- Date: 2016
- Subjects: Mycobacterium tuberculosis -- South Africa -- Eastern Cape Public health -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10353/13238 , vital:39625
- Description: Tuberculosis (TB) is a disease caused by the members of the Mycobacterium tuberculosis complex (MTBC), which infects both animals and humans. The primary host of Mycobacterium bovis is cattle. It remains a major global health problem. About 2 billion people are thought to be infected with TB and about 1.3 million decease each year from the disease. This study is aimed at determining the prevalence of the disease, characterizing the MTBC isolates and increasing the understanding of the genetic diversity MTBC in the Eastern Cape Province of South Africa, from studying both samples from both humans and cattle. A total of 376 cattle lymph nodes were collected from two commercial abattoirs for investigations. Suspected 6 000 sputum specimens were also collected from humans (cattle farmers). All collected samples were cultured, and isolates were confirmed with a multiplex polymerase chain reaction (PCR) targeting the mpb64 and IS6110 genes. Positive isolates were tested for resistance against anti-TB drugs using the standard Lowenstein Jensen proportion method. Isolates from cattle lymph nodes were further characterized to species level, using the region of deletion 1 (RD1) and oxyR restriction fragment length polymorphism analyses. MIRU-VNTR typing and spoligotyping assays were used to further examine the genetic diversity of all strains. Obtained spoligotype patterns were matched with isolates published in the universal spoligotyping database (SITVIT2). MTBC was detected in 162 (43.09 percent) cattle lymph nodes and 156 sputum specimens. Some isolates 25.9 percent (42/162) from the cattle lymph nodes samples were resistant to rifampicin (RIF) and 98.7 percent (154/156) were resistant to isoniazid (INH) and rifampicin (RIF) (of the human sputum specimens). All cattle lymph nodes isolates exhibited the RD1 gene, and the oxyR analyses classified 96.9 percent of the isolates as Mycobacterium tuberculosis complex and 3.1 percent as Mycobacterium bovis. Twenty seven spoligotype patterns were isolated from cattle lymph nodes, with 10 shared types (SIT) consigned to 5 lineages, including Bov_4-Caprea (1.9 percent), Microti (0.6 percent) and Beijing (17.9 percent) constituting East Asian, Latin-American-Mediterranean (LAM) (3.7 percent), X (4.3 percent), MANU (3.1percent), S (0.6 percent) and 67.9 percent were orphans that were further categorized to their designated lineages (Indo-Oceanic, Euro-American and West-African 2). Ninety one percent of the isolates from humans (farmers) were grouped into 7 clusters by spoligotyping, while the remaining 9 percent was unclustered. SITs were observed in 89.6 percent of the isolates from the Beijing, LAM, X, T and S families. The Beijing family was the most prevalent strain from both human isolates (77.2 percent) and cattle lymph nodes isolates (17.9 percent), and the T (0.6 percent) and Microti (0.6 percent) families were the least detected genotypes in both humans and cattle lymph nodes. In the cattle lymph nodes, spoligotyping showed a higher clustering rate of 82.1 percent, with the lowest (HGDI) = 0.485. The 12 MIRU-VNTR clustering rate was 64.8 percent, showing a higher HGDI of 0.671. However, a combination of both methods showed a decreased clustering rate of 53.7 percent; however these methods resulted in a higher HGDI (0.676). Isolates from human sputum specimens spoligotyping showed a higher clustering rate of 89 percent with an HGDI = 0.822. The 12 MIRU-VNTR showed the lowest clustering rate of 69 percent and an HGDI = 0.940 and the combination of the methods showed a clustering rate of 78 percent and an HGDI = 0.951. The results of this study show the diversity of MTBC strains in the Eastern Cape Province and the low clustering rate indicates continuing transmission in the province. The detection of MTBC strains from slaughtered cattle lymph nodes in abattoirs confirms the risk of providing TB infected meat in markets which has serious implications for the control of TB in South Africa. The continuing spread of MTBC in South Africa threatens both the public and economic health.
- Full Text:
- Date Issued: 2016
Monitoring changes in vegetation distribution to ascertain the extent of degradation in the savannas of Nkonkobe Local Municipality, Eastern Cape, South Africa
- Authors: Masiza, Wonga
- Date: 2016
- Subjects: Savanna ecology -- South Africa -- Eastern Cape Land degradation -- South Africa -- Eastern Cape Vegetation management -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/2027 , vital:27598
- Description: Savanna degradation is an environmental problem occurring in most countries around the world and it poses threats to biodiversity conservation, the food industry, and other economic sectors. According to FAO, South Africa’s rangelands exhibit the highest rate of fragmentation in comparison to range ecosystems in neighbouring countries including Lesotho and Swaziland, and consensus among researchers is that communal rangelands are more degraded than commercial rangelands. Although researchers and communities have identified the occurrence of land degradation in communal savannas at a local scale, land degradation has been poorly estimated because little has been done to quantify the extent and dynamics of perceived and observed changes associated with land degradation. The main goal of this study is to provide empirical insights on the direction of changes in the communal savannas of Nkonkobe Local Municipality in order to inform policy formulation and implementation. Additional to the communal sites is a private farm included for comparative analysis of trends in communal and commercial savannas. Landsat imagery was used to map, assess, and quantify the extent of land degradation in Nkonkobe Local Municipality, over a period of 30 years between 1984 and 2014. Field investigations were undertaken in June 2015 to acquire reference data to guide supervised classification of Landsat images. Three algorithms (Mahalanobis-distance, Minimum-distance, and Maximum likelihood classification) were compared to identify a classifier that produced the best results. The maximum likelihood classifier produced the best results with classification accuracy levels of 95.24 percent, 89.66 percent, and 95.65 percent for Honeydale Farm, Thyume, and Sheshegu respectively. Regression analysis revealed that both communal and private lands have experienced statistically significant increases in bush encroachment and decreases in surface water. Communal savannas have been confronted more by expansion of built-up area, decrease in open grassland, abandonment of arable land, soil erosion, and a steady invasion by Acacia Karroo compared to the privately owned commercial farm. The land cover changes measured through this investigation suggest an environmental shift that threatens biodiversity and agricultural activity. The study provides empirically informed insights about the direction to which these savannas are changing with the hope that the findings will prompt formulation and implementation of effective policies.
- Full Text:
- Date Issued: 2016
- Authors: Masiza, Wonga
- Date: 2016
- Subjects: Savanna ecology -- South Africa -- Eastern Cape Land degradation -- South Africa -- Eastern Cape Vegetation management -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/2027 , vital:27598
- Description: Savanna degradation is an environmental problem occurring in most countries around the world and it poses threats to biodiversity conservation, the food industry, and other economic sectors. According to FAO, South Africa’s rangelands exhibit the highest rate of fragmentation in comparison to range ecosystems in neighbouring countries including Lesotho and Swaziland, and consensus among researchers is that communal rangelands are more degraded than commercial rangelands. Although researchers and communities have identified the occurrence of land degradation in communal savannas at a local scale, land degradation has been poorly estimated because little has been done to quantify the extent and dynamics of perceived and observed changes associated with land degradation. The main goal of this study is to provide empirical insights on the direction of changes in the communal savannas of Nkonkobe Local Municipality in order to inform policy formulation and implementation. Additional to the communal sites is a private farm included for comparative analysis of trends in communal and commercial savannas. Landsat imagery was used to map, assess, and quantify the extent of land degradation in Nkonkobe Local Municipality, over a period of 30 years between 1984 and 2014. Field investigations were undertaken in June 2015 to acquire reference data to guide supervised classification of Landsat images. Three algorithms (Mahalanobis-distance, Minimum-distance, and Maximum likelihood classification) were compared to identify a classifier that produced the best results. The maximum likelihood classifier produced the best results with classification accuracy levels of 95.24 percent, 89.66 percent, and 95.65 percent for Honeydale Farm, Thyume, and Sheshegu respectively. Regression analysis revealed that both communal and private lands have experienced statistically significant increases in bush encroachment and decreases in surface water. Communal savannas have been confronted more by expansion of built-up area, decrease in open grassland, abandonment of arable land, soil erosion, and a steady invasion by Acacia Karroo compared to the privately owned commercial farm. The land cover changes measured through this investigation suggest an environmental shift that threatens biodiversity and agricultural activity. The study provides empirically informed insights about the direction to which these savannas are changing with the hope that the findings will prompt formulation and implementation of effective policies.
- Full Text:
- Date Issued: 2016
Moringa oleifera leaf powder as a functional antioxidant additive in pork droewors
- Authors: Mukumbo, Felicitas Esnart
- Date: 2016
- Subjects: Antioxidants , Moringa oleifera , Pork -- Quality
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10353/15354 , vital:40400
- Description: The study investigated the effect of Moringa oleifera leaf powder (MLP) on physico-chemical characteristics, antioxidant activity, antioxidant compound content and lipid oxidation in pork droëwors. Firstly, the physico-chemical properties (proximate composition, salt content, water activity (aw), pH) of commercially produced droëwors from different types of meat (beef, game, ostrich) were determined. In the second experiment beef and pork droëwors with similar fat content were produced. Physico-chemical properties and lipid oxidation (thiobarbituric reactive substances (TBARS)) during processing and 26 days of storage at 25 °C and 50percent relative humidity (RH) were measured. In the third experiment, antioxidant compounds (Total Phenolic Compounds (TPC), α-tocopherol, β-carotene) in MLP were quantified. Thereafter, 4 treatments of pork droëwors were produced, with MLP included at 0, 0.5, 1 and 2 g/ 100 g. Physico-chemical properties and TBARS were measured at intervals during drying (0, 1.5, 5.75, 27.25, 72 h) and after 7 days of storage under ambient conditions. Antioxidant activity (ferric reducing antioxidant power (FRAP)), TBARS, α- and γ-tocopherol, and β-carotene contents were measured. In the fourth experiment, three batches of droëwors were produced (C: no antioxidant, M0.75: 0.75 g/ 100 g MLP, VE: 15 mg/ kg α-tocopherol oil) and stored at 25 °C and 50percent relative humidity for 112 days. Drying kinetics and α-tocopherol contents of pork droëwors after drying were measured and the physico-chemical properties and TBARS were followed during storage. Results showed no differences (P > 0.05) in the physico-chemical characteristics of beef, game meat and ostrich droëwors; containing on average 25.8 ± 1.25 g/100 g moisture, 42.0 ± 0.10 g/100 g protein, 32.0 ± 1.68 g/100 g fat, 6.2 ± 0.13 g/100 g ash and 4.2 ± 0.10 g/100 g salt; with aw and pH of 0.79 ± 0.015 and 5.3 ± 0.05, respectively. During processing and storage, TBARS were higher (P < 0.05) in pork droëwors (maximum 3.83 mg MDA/kg DM) than in beef (maximum 0.99 mg MDA/kg DM). Moringa oleifera leaf powder contained high levels of TPC (7.5 ± 0.2 mg gallic acid eq/g) and substantial levels of α-tocopherol (76.7 ± 1.9 mg/100 g) and β-carotene (23.2 ± 2.8 mg/100 g). The FRAP, α-tocopherol and β-carotene content of pork droëwors increased (P < 0.05) proportionally with increasing levels of MLP inclusion. Lipid oxidation occurred more rapidly (P < 0.05) when MLP was not added and was similar (P > 0.05) for all MLP treatments. There was no significant effect of the inclusion of 0.75 g/100 g MLP on the drying curves and physico-chemical characteristics of the droëwors. The α-tocopherol content was higher (P < 0.05) and TBARS during storage were lower (P > 0.05) with MLP addition. The results of the current study give an overview of the composition of commercial droëwors and showed higher susceptibility to lipid oxidation in pork droëwors. Moringa oleifera leaf powder exhibited antioxidant activity in pork droëwors, inhibited lipid oxidation and increased the content of α-tocopherol in the product. It can be concluded that MLP could be used as a functional antioxidant additive in pork droëwors.
- Full Text:
- Date Issued: 2016
- Authors: Mukumbo, Felicitas Esnart
- Date: 2016
- Subjects: Antioxidants , Moringa oleifera , Pork -- Quality
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10353/15354 , vital:40400
- Description: The study investigated the effect of Moringa oleifera leaf powder (MLP) on physico-chemical characteristics, antioxidant activity, antioxidant compound content and lipid oxidation in pork droëwors. Firstly, the physico-chemical properties (proximate composition, salt content, water activity (aw), pH) of commercially produced droëwors from different types of meat (beef, game, ostrich) were determined. In the second experiment beef and pork droëwors with similar fat content were produced. Physico-chemical properties and lipid oxidation (thiobarbituric reactive substances (TBARS)) during processing and 26 days of storage at 25 °C and 50percent relative humidity (RH) were measured. In the third experiment, antioxidant compounds (Total Phenolic Compounds (TPC), α-tocopherol, β-carotene) in MLP were quantified. Thereafter, 4 treatments of pork droëwors were produced, with MLP included at 0, 0.5, 1 and 2 g/ 100 g. Physico-chemical properties and TBARS were measured at intervals during drying (0, 1.5, 5.75, 27.25, 72 h) and after 7 days of storage under ambient conditions. Antioxidant activity (ferric reducing antioxidant power (FRAP)), TBARS, α- and γ-tocopherol, and β-carotene contents were measured. In the fourth experiment, three batches of droëwors were produced (C: no antioxidant, M0.75: 0.75 g/ 100 g MLP, VE: 15 mg/ kg α-tocopherol oil) and stored at 25 °C and 50percent relative humidity for 112 days. Drying kinetics and α-tocopherol contents of pork droëwors after drying were measured and the physico-chemical properties and TBARS were followed during storage. Results showed no differences (P > 0.05) in the physico-chemical characteristics of beef, game meat and ostrich droëwors; containing on average 25.8 ± 1.25 g/100 g moisture, 42.0 ± 0.10 g/100 g protein, 32.0 ± 1.68 g/100 g fat, 6.2 ± 0.13 g/100 g ash and 4.2 ± 0.10 g/100 g salt; with aw and pH of 0.79 ± 0.015 and 5.3 ± 0.05, respectively. During processing and storage, TBARS were higher (P < 0.05) in pork droëwors (maximum 3.83 mg MDA/kg DM) than in beef (maximum 0.99 mg MDA/kg DM). Moringa oleifera leaf powder contained high levels of TPC (7.5 ± 0.2 mg gallic acid eq/g) and substantial levels of α-tocopherol (76.7 ± 1.9 mg/100 g) and β-carotene (23.2 ± 2.8 mg/100 g). The FRAP, α-tocopherol and β-carotene content of pork droëwors increased (P < 0.05) proportionally with increasing levels of MLP inclusion. Lipid oxidation occurred more rapidly (P < 0.05) when MLP was not added and was similar (P > 0.05) for all MLP treatments. There was no significant effect of the inclusion of 0.75 g/100 g MLP on the drying curves and physico-chemical characteristics of the droëwors. The α-tocopherol content was higher (P < 0.05) and TBARS during storage were lower (P > 0.05) with MLP addition. The results of the current study give an overview of the composition of commercial droëwors and showed higher susceptibility to lipid oxidation in pork droëwors. Moringa oleifera leaf powder exhibited antioxidant activity in pork droëwors, inhibited lipid oxidation and increased the content of α-tocopherol in the product. It can be concluded that MLP could be used as a functional antioxidant additive in pork droëwors.
- Full Text:
- Date Issued: 2016
Normative indicators for grade 3 and grade 7 isiXhosa-speaking children on the Bender Visual-Motor Gestalt Test (Second Edition)
- Authors: Palmer, Hanli
- Date: 2016
- Subjects: Bender-Gestalt Test Psychological tests for children
- Language: English
- Type: Thesis , Masters , MSoc
- Identifier: http://hdl.handle.net/10353/6346 , vital:29583
- Description: Selecting appropriate normative data for the purpose of evaluating psychometric test scores forms an integral part of the interpretative psychological assessment process. This highlights the well-known problem of utilising measures developed in the United Kingdom (UK), or the United States (US) and accepting their norms unreservedly for the practice on local populations, which questions the tests’ validity and reliability. The current study, which aimed to collect normative indicators on the Bender Gestalt Test (Second Edition), forms part of the continuing effort to develop norms for psychological tests in South Africa, and to focus on the valid and reliable use of measures within the context of South Africa’s multicultural and multilingual population groups. The participants of the research were Black isiXhosa-speaking Grade 3 and Grade 7 learners aged 8 to 9 years (N = 38 ), and 12 to 13 years (N = 23), obtaining their school education in the disadvantaged educational setting of the former Department of Education and Training (ex-DET) schools. The tests were scored according to the Bender Gestalt Test (Second Edition) manual, using the Global Scoring System. Thereafter, the raw scores were converted to Standard Scores, T-scores and Percentile Ranks using the US normative tables, according to chronological age categories. When the two groups were compared to the US norms, the results equated favourably for the present study sample. There were no significant findings in relation to the classroom size or any difference in performance between the schools who participated. The only significant difference revealed between male and female participants was with the Grade 7 sample group, where the males scored significantly lower than females on the Recall phase. These results support the prospect of the continuous revision of norms, and it is suggesting that the cognitive processes measured by this test are likely to derive from robust neurological substrates that are relatively stable across cultural groups.
- Full Text:
- Date Issued: 2016
- Authors: Palmer, Hanli
- Date: 2016
- Subjects: Bender-Gestalt Test Psychological tests for children
- Language: English
- Type: Thesis , Masters , MSoc
- Identifier: http://hdl.handle.net/10353/6346 , vital:29583
- Description: Selecting appropriate normative data for the purpose of evaluating psychometric test scores forms an integral part of the interpretative psychological assessment process. This highlights the well-known problem of utilising measures developed in the United Kingdom (UK), or the United States (US) and accepting their norms unreservedly for the practice on local populations, which questions the tests’ validity and reliability. The current study, which aimed to collect normative indicators on the Bender Gestalt Test (Second Edition), forms part of the continuing effort to develop norms for psychological tests in South Africa, and to focus on the valid and reliable use of measures within the context of South Africa’s multicultural and multilingual population groups. The participants of the research were Black isiXhosa-speaking Grade 3 and Grade 7 learners aged 8 to 9 years (N = 38 ), and 12 to 13 years (N = 23), obtaining their school education in the disadvantaged educational setting of the former Department of Education and Training (ex-DET) schools. The tests were scored according to the Bender Gestalt Test (Second Edition) manual, using the Global Scoring System. Thereafter, the raw scores were converted to Standard Scores, T-scores and Percentile Ranks using the US normative tables, according to chronological age categories. When the two groups were compared to the US norms, the results equated favourably for the present study sample. There were no significant findings in relation to the classroom size or any difference in performance between the schools who participated. The only significant difference revealed between male and female participants was with the Grade 7 sample group, where the males scored significantly lower than females on the Recall phase. These results support the prospect of the continuous revision of norms, and it is suggesting that the cognitive processes measured by this test are likely to derive from robust neurological substrates that are relatively stable across cultural groups.
- Full Text:
- Date Issued: 2016
Occupational stress and intrinsic motivation as determinants of job engagement in the South African Police Service in Greater Kokstad Municipality
- Authors: Jojo, Avela
- Date: 2016
- Subjects: Police -- Job stress -- South Africa Employee motivation -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MCom
- Identifier: http://hdl.handle.net/10353/1995 , vital:27592
- Description: Occupational stress, intrinsic motivation and job engagement are areas of importance for today’s management. The main objective of this study was to explore the combined effect of occupational stress and intrinsic motivation in determining job engagement in the South African Police Service. Moreover, the study investigated the relationship between occupational stress, intrinsic motivation and job engagement. The study also sought to enquire into the effects rank and gender has on the study variables. A quantitative approach was adopted. A structured self – administered questionnaire comprising of four sections: biographical information, police stress questionnaire, intrinsic motivation inventory and job engagement scale was used to collect data. Statistical Package for the Social Sciences (SPSS) was used to analyse data, and both descriptive and inferential statistics were utilized. The results revealed a negative relationship between occupational stress and job engagement. A positive relationship between intrinsic motivation and job engagement was also found. There was no combined effect of occupational stress and intrinsic motivation on job engagement. Lastly, no significant differences in the way police experience stress were found among rank and gender. The researcher concluded that even when police officers are under great amounts of stress they are still engaged in their jobs. Where levels of intrinsic motivation were high for the police officers, so were the levels of job engagement. Demographic variables did not yield any significant relationships to the study variables. These findings are of significance in creating a police force which is productive and meets the goals and objectives of the South African Police Service.
- Full Text:
- Date Issued: 2016
- Authors: Jojo, Avela
- Date: 2016
- Subjects: Police -- Job stress -- South Africa Employee motivation -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MCom
- Identifier: http://hdl.handle.net/10353/1995 , vital:27592
- Description: Occupational stress, intrinsic motivation and job engagement are areas of importance for today’s management. The main objective of this study was to explore the combined effect of occupational stress and intrinsic motivation in determining job engagement in the South African Police Service. Moreover, the study investigated the relationship between occupational stress, intrinsic motivation and job engagement. The study also sought to enquire into the effects rank and gender has on the study variables. A quantitative approach was adopted. A structured self – administered questionnaire comprising of four sections: biographical information, police stress questionnaire, intrinsic motivation inventory and job engagement scale was used to collect data. Statistical Package for the Social Sciences (SPSS) was used to analyse data, and both descriptive and inferential statistics were utilized. The results revealed a negative relationship between occupational stress and job engagement. A positive relationship between intrinsic motivation and job engagement was also found. There was no combined effect of occupational stress and intrinsic motivation on job engagement. Lastly, no significant differences in the way police experience stress were found among rank and gender. The researcher concluded that even when police officers are under great amounts of stress they are still engaged in their jobs. Where levels of intrinsic motivation were high for the police officers, so were the levels of job engagement. Demographic variables did not yield any significant relationships to the study variables. These findings are of significance in creating a police force which is productive and meets the goals and objectives of the South African Police Service.
- Full Text:
- Date Issued: 2016
Perceptions of indigenous people regarding mental illness at Cacadu district in the Eastern Cape province of South Africa
- Authors: Tilolo, Lwazi
- Date: 2016
- Language: English
- Type: Thesis , Masters , MSc (Nursing Science)
- Identifier: vital:11930 , http://hdl.handle.net/10353/d1021323
- Description: Indigenous people tend to consult traditional healers when a family member manifests change in behaviour, whilst conventional treatment disregards spirituality when preserving mental health. The aim of the study was to explore the perceptions of indigenous people and the role of traditional healers in the management of mentally ill persons within the Cacadu District in the Eastern Cape Province of South Africa. The study has adopted a qualitative approach which was exploratory and descriptive in nature. The sample consisted of nine relatives of mental health care users (MHCUs) and six traditional healers. Ethical principles were also taken into consideration by the researcher during the process of conducting the study. Data were collected from two strata, namely, relatives of the MHCUs and the traditional healers and an interview guide was used to conduct in-depth face-to-face interviews. Data were analysed using Tesch’s method of data analysis. Four themes emerged from the data categories and sub categories were identified. According to the themes participants indicated the negative impact of mental illness; as a result they portrayed great desperation regarding the means of accessing a cure for mental illness. Some participants showed insufficient knowledge regarding mental illness and had different perceptions and beliefs regarding the origin of mental illness. Available literature was used to emphasise and support the views that were expressed by both traditional healers and relatives of MHCUs. It has been highlighted from this study that indigenous people of Cacadu District view mental illness as spiritual in origin but they include Western medication for the benefit of the mentally ill. In addition, the relatives of the MHCUs highlighted the economic burden as the major problem that results from mental illness.
- Full Text:
- Date Issued: 2016
- Authors: Tilolo, Lwazi
- Date: 2016
- Language: English
- Type: Thesis , Masters , MSc (Nursing Science)
- Identifier: vital:11930 , http://hdl.handle.net/10353/d1021323
- Description: Indigenous people tend to consult traditional healers when a family member manifests change in behaviour, whilst conventional treatment disregards spirituality when preserving mental health. The aim of the study was to explore the perceptions of indigenous people and the role of traditional healers in the management of mentally ill persons within the Cacadu District in the Eastern Cape Province of South Africa. The study has adopted a qualitative approach which was exploratory and descriptive in nature. The sample consisted of nine relatives of mental health care users (MHCUs) and six traditional healers. Ethical principles were also taken into consideration by the researcher during the process of conducting the study. Data were collected from two strata, namely, relatives of the MHCUs and the traditional healers and an interview guide was used to conduct in-depth face-to-face interviews. Data were analysed using Tesch’s method of data analysis. Four themes emerged from the data categories and sub categories were identified. According to the themes participants indicated the negative impact of mental illness; as a result they portrayed great desperation regarding the means of accessing a cure for mental illness. Some participants showed insufficient knowledge regarding mental illness and had different perceptions and beliefs regarding the origin of mental illness. Available literature was used to emphasise and support the views that were expressed by both traditional healers and relatives of MHCUs. It has been highlighted from this study that indigenous people of Cacadu District view mental illness as spiritual in origin but they include Western medication for the benefit of the mentally ill. In addition, the relatives of the MHCUs highlighted the economic burden as the major problem that results from mental illness.
- Full Text:
- Date Issued: 2016
Perspectives of Post Graduate Certificate in Education (PGCE) students on the effectiveness of school based mentoring
- Authors: Baartman, Nomakhaya
- Date: 2016
- Subjects: Mentoring in education -- South Africa -- Eastern Cape Teachers -- In-service training -- South Africa -- Eastern Cape Student teachers -- Supervision of -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: http://hdl.handle.net/10353/5641 , vital:29354
- Description: Effective mentoring is essential for the development of student-teachers. A sequential explanatory mixed method study was conducted in order to analyse the perspectives of Post Graduate Certificate of Education (PGCE) students on the effectiveness of school based mentoring. Thirty-six (36) PGCE students from a higher education institution in the Eastern Cape participated in this study. Firstly, quantitative questionnaires were used to gather data from all the participants followed by qualitative semi–structured interviews from a purposive sample of five (5) participants in order to enrich the study. This study analysed PGCE students’ perspectives of mentor teacher practices. In doing so the study evoked Hudson and Peards’ Five Factor Mentoring Model. This model includes Personal Attributes, Systems Requirements, Pedagogical Knowledge, Modelling and Feedback. From the findings, PGCE students reported that Teaching Practice (TP) is a stressful period full of anxieties, excitement and fears, hence they need to be guided and supported by knowledgeable and specialist teachers (mentors). Mentors play a significant role in supporting and guiding student-teachers during TP. From the analysis of PGCE students’ perspectives, the research suggested that those who were supported and guided by their mentors experienced positive mentoring during TP. Those who experienced negative mentoring reported limited time for mentoring and unavailability of the mentors as the causes. They further expressed that they wished mentors were understanding, good models who treated them as teacher candidates not as students and gave them constructive feedback. Finally, they indicated that the partnership between host schools and the university needs to be improved.
- Full Text:
- Date Issued: 2016
- Authors: Baartman, Nomakhaya
- Date: 2016
- Subjects: Mentoring in education -- South Africa -- Eastern Cape Teachers -- In-service training -- South Africa -- Eastern Cape Student teachers -- Supervision of -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: http://hdl.handle.net/10353/5641 , vital:29354
- Description: Effective mentoring is essential for the development of student-teachers. A sequential explanatory mixed method study was conducted in order to analyse the perspectives of Post Graduate Certificate of Education (PGCE) students on the effectiveness of school based mentoring. Thirty-six (36) PGCE students from a higher education institution in the Eastern Cape participated in this study. Firstly, quantitative questionnaires were used to gather data from all the participants followed by qualitative semi–structured interviews from a purposive sample of five (5) participants in order to enrich the study. This study analysed PGCE students’ perspectives of mentor teacher practices. In doing so the study evoked Hudson and Peards’ Five Factor Mentoring Model. This model includes Personal Attributes, Systems Requirements, Pedagogical Knowledge, Modelling and Feedback. From the findings, PGCE students reported that Teaching Practice (TP) is a stressful period full of anxieties, excitement and fears, hence they need to be guided and supported by knowledgeable and specialist teachers (mentors). Mentors play a significant role in supporting and guiding student-teachers during TP. From the analysis of PGCE students’ perspectives, the research suggested that those who were supported and guided by their mentors experienced positive mentoring during TP. Those who experienced negative mentoring reported limited time for mentoring and unavailability of the mentors as the causes. They further expressed that they wished mentors were understanding, good models who treated them as teacher candidates not as students and gave them constructive feedback. Finally, they indicated that the partnership between host schools and the university needs to be improved.
- Full Text:
- Date Issued: 2016
Pharmacological studies of Foeniculum Vulgare (Mill.) and Lippia Javanica (Burm.F.) spreng. used as spices in Nkonkobe Municipality of the Eastern Cape Province, South Africa
- Asowata-Ayodele, Abiola Mojisola
- Authors: Asowata-Ayodele, Abiola Mojisola
- Date: 2016
- Subjects: Medicinal plants -- South Africa -- Eastern Cape Fennel -- Utilization -- South Africa -- Eastern Cape Ethnopharmacology -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10353/2777 , vital:28085
- Description: Spices are of great importance in the indigenous culinary and traditional medicine systems of the people of Nkonkobe Municipality of the Eastern Cape, South Africa. The present investigation evaluated the ethnopharmacological potentials of two indigenous South African spices- Foeniculum vulgare and Lippia javanica. The pharmacological investigations on these two plant species include ultra-morphology, nutrient and mineral analysis, evaluation of the essential oil, phytochemical and antioxidant assays, antimicrobial, anti-inflammatory as well as anti-urolithiatic assay of the acetone and aqueous extracts of the two plants. The species were selected for study as the most cited plants after an ethnobotanical survey conducted on the indigenous knowledge of plants used as spices and medicine in Nkonkobe Municipality, Eastern Cape of South Africa. Among the plants cited Foeniculum vulgare(Apiaceae) and Lippia javanica (Verbenaceae) stood out as the most commonly used spices. Others were members of the families; Solanaceae, Apiaceae, Amaryllidaceae, Amaranthaceae and Lamiaceae. Ultra-morphological studies conducted on the leaves of the two selected plants using scanning electron microscope revealed the presence of non-glandular and glandular trichomes, stomata and crystals. The leaf surfaces of these herbs may serve as secretory sites where aromatic secondary metabolites are produced. Analyses of the proximate, mineral, vitamin and anti-nutrients contents of these two spices showed that both species are good sources of these phytochemicals and may be used to enrich the human diet. Lippia javanica possesses lower lipid (0.50 percent), fibre (5 percent) and carbohydrate (64.96 percent) contents than Foeniculum vulgare. On the other hand, protein (20.54 percent), ash (11.60 percent) and moisture content (11.69 percent) were higher in Foeniculum vulgare than in Lippia javanica. Foeniculum vulgare showed higher N (3286 mg/100g), Mg (386.7 mg/100g), K (3187 mg/100g) and Na (1383 mg/100g) content while Lippia javanica was higher in Ca (1833 mg/100g), Zn (4.7 mg/100g), Cu (0.9 mg/100g) and Fe (78.4 mg/100g). Vitamins Aand E were also higher in Lippia javanica (1.31 mg/100g; 2.52 mg/100g) while Foeniculum vulgare (0.45 mg/100g) had higher vitamin C content. No significant differences were observed in the phytate, oxalate and tannin contents of the two spices, but saponin and cyanide were significantly lower in Lippia javanica (268.5 mg/100g; 8.45 mg/100g) than in Foeniculum vulgare (1855 mg/100g; 10.5 mg/100g) Evaluation of the essential oil component, cytotoxicity and antimicrobial activities of both fresh and dried leaves of Lippia javanica and Foeniculum vulgare revealed that the dried samples yielded more oil and also contain more chemical than the fresh samples of both plants. The overall antimicrobial activity evaluated using susceptibility and microdilution assays revealed that the oils of F. vulgare and L. javanica exhibited high antifungal and antibacterial activity, compared to the reference drugs. In addition, essential oil from fresh leaves of both spices was less toxic compared to the oil from the dried leaves. Polyphenolic evaluation of the aqueous and acetone extracts of the plants revealed that the acetone extract had higher phenol, flavanol, flavonoid and proanthocyanidin contents than the aqueous extracts. The total phenolic content of acetone and aqueous extracts were 4.49 ± 0.411 mg/g and 3.73 ± 0.498 mg/g tannic acid equivalent (TAE) respectively for Lippia javanica. The same trend was also observed in Foeniculum vulgare with total phenolic content of acetone and aqueous extracts were 4.22 ± 0.325 mg/g and 4.17 ± 0.651 mg/g tannic acid equivalent (TAE) respectively. Further assessment of the antioxidant activity of the solvent extracts revealed that both plants exhibited promising free radical scavenging potentials against 1,1 diphenyl-2-picrylhydrazyl (DPPH), 2, 2’-azino-bis(3- ethylbenzthiazoline-6-sulfonic acid (ABTS), reducing power, lipid peroxidation, nitric oxide, phosphomolybdate and hydrogen peroxide. Antimicrobial activities of the acetone and aqueous extracts of the two plants revealed moderate antibacterial and antifungal activities. They inhibited the growth of Microsporium canis and Trichophyton rubrum that were not susceptible to the standard antifungal drug used as control.
- Full Text:
- Date Issued: 2016
- Authors: Asowata-Ayodele, Abiola Mojisola
- Date: 2016
- Subjects: Medicinal plants -- South Africa -- Eastern Cape Fennel -- Utilization -- South Africa -- Eastern Cape Ethnopharmacology -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10353/2777 , vital:28085
- Description: Spices are of great importance in the indigenous culinary and traditional medicine systems of the people of Nkonkobe Municipality of the Eastern Cape, South Africa. The present investigation evaluated the ethnopharmacological potentials of two indigenous South African spices- Foeniculum vulgare and Lippia javanica. The pharmacological investigations on these two plant species include ultra-morphology, nutrient and mineral analysis, evaluation of the essential oil, phytochemical and antioxidant assays, antimicrobial, anti-inflammatory as well as anti-urolithiatic assay of the acetone and aqueous extracts of the two plants. The species were selected for study as the most cited plants after an ethnobotanical survey conducted on the indigenous knowledge of plants used as spices and medicine in Nkonkobe Municipality, Eastern Cape of South Africa. Among the plants cited Foeniculum vulgare(Apiaceae) and Lippia javanica (Verbenaceae) stood out as the most commonly used spices. Others were members of the families; Solanaceae, Apiaceae, Amaryllidaceae, Amaranthaceae and Lamiaceae. Ultra-morphological studies conducted on the leaves of the two selected plants using scanning electron microscope revealed the presence of non-glandular and glandular trichomes, stomata and crystals. The leaf surfaces of these herbs may serve as secretory sites where aromatic secondary metabolites are produced. Analyses of the proximate, mineral, vitamin and anti-nutrients contents of these two spices showed that both species are good sources of these phytochemicals and may be used to enrich the human diet. Lippia javanica possesses lower lipid (0.50 percent), fibre (5 percent) and carbohydrate (64.96 percent) contents than Foeniculum vulgare. On the other hand, protein (20.54 percent), ash (11.60 percent) and moisture content (11.69 percent) were higher in Foeniculum vulgare than in Lippia javanica. Foeniculum vulgare showed higher N (3286 mg/100g), Mg (386.7 mg/100g), K (3187 mg/100g) and Na (1383 mg/100g) content while Lippia javanica was higher in Ca (1833 mg/100g), Zn (4.7 mg/100g), Cu (0.9 mg/100g) and Fe (78.4 mg/100g). Vitamins Aand E were also higher in Lippia javanica (1.31 mg/100g; 2.52 mg/100g) while Foeniculum vulgare (0.45 mg/100g) had higher vitamin C content. No significant differences were observed in the phytate, oxalate and tannin contents of the two spices, but saponin and cyanide were significantly lower in Lippia javanica (268.5 mg/100g; 8.45 mg/100g) than in Foeniculum vulgare (1855 mg/100g; 10.5 mg/100g) Evaluation of the essential oil component, cytotoxicity and antimicrobial activities of both fresh and dried leaves of Lippia javanica and Foeniculum vulgare revealed that the dried samples yielded more oil and also contain more chemical than the fresh samples of both plants. The overall antimicrobial activity evaluated using susceptibility and microdilution assays revealed that the oils of F. vulgare and L. javanica exhibited high antifungal and antibacterial activity, compared to the reference drugs. In addition, essential oil from fresh leaves of both spices was less toxic compared to the oil from the dried leaves. Polyphenolic evaluation of the aqueous and acetone extracts of the plants revealed that the acetone extract had higher phenol, flavanol, flavonoid and proanthocyanidin contents than the aqueous extracts. The total phenolic content of acetone and aqueous extracts were 4.49 ± 0.411 mg/g and 3.73 ± 0.498 mg/g tannic acid equivalent (TAE) respectively for Lippia javanica. The same trend was also observed in Foeniculum vulgare with total phenolic content of acetone and aqueous extracts were 4.22 ± 0.325 mg/g and 4.17 ± 0.651 mg/g tannic acid equivalent (TAE) respectively. Further assessment of the antioxidant activity of the solvent extracts revealed that both plants exhibited promising free radical scavenging potentials against 1,1 diphenyl-2-picrylhydrazyl (DPPH), 2, 2’-azino-bis(3- ethylbenzthiazoline-6-sulfonic acid (ABTS), reducing power, lipid peroxidation, nitric oxide, phosphomolybdate and hydrogen peroxide. Antimicrobial activities of the acetone and aqueous extracts of the two plants revealed moderate antibacterial and antifungal activities. They inhibited the growth of Microsporium canis and Trichophyton rubrum that were not susceptible to the standard antifungal drug used as control.
- Full Text:
- Date Issued: 2016
Population, communication and habitat comparisons between the major gummivorous strepsirhines of Madagascar (phaner) and Cameroon (euoticus)
- Authors: Forbanka, Derick Nomuh
- Date: 2016
- Subjects: Adaptation (Biology) , Mammals -- Evolution , Mammals -- Behavior
- Language: English
- Type: Thesis , Doctoral , Zoology
- Identifier: http://hdl.handle.net/10353/15957 , vital:40567
- Description: The suborder Strepsirhini contains a large proportion of the living gummivorous mammal taxa, and includes two genera of specialist gummivores, Euoticus and Phaner, which present particularly interesting cases of convergent adaptive evolution in Africa and Madagascar, respectively. Although these genera are not closely related, they share several anatomical, behavioural and physiological specialisations associated with their diets, and despite their biogeographical separation, there seems to be substantial coincidence in their habitats and habits. Neither the ecology nor the systematics of Phaner and Euoticus has been the subject of much detailed research, and little is known of the conservation status of the genera. Even their specific diversity is unresolved. In this study I investigated and compared aspects of their ecology at both a macro-and a microhabitat scale, including wild populations of all groups within the two genera that have been accorded species status in recent field guides and systematic reviews. I also studied aspects of their social communication behaviour as clues to their species-level diversity. Species and their formation (or speciation) have inspired a great deal of evolutionary research, but remain contentious issues in evolutionary biology. This is due both to the difficulties inherent in inferring the details of a dynamic biological process that occurred over some time in the past from the recent, relatively static patterns of variation observed, as well as the limited consensus among biologists in identifying such patterns. It is therefore important to distinguish the kinds of patterns in nature that are predicted by different views of species and speciation, and also to see how well these predictions fare when applied to the speciation patterns found in strepsirhine primates. Theories of speciation cannot be separated from species concepts, and one concept widely supported in primatology is the Recognition Concept of species because of its heuristic nature. The Recognition Concept is based on Specific-mate Recognition Systems (SMRS). I focussed on SMRS features of Euoticus and Phaner, loud call specificity, and species’ preferred habitats. My results provided some support for the distinctiveness of currently identified species, although this was not clear-cut. Population densities of both Phaner and Euoticus appear to have declined in recent years, even though both Phaner and Euoticus appear to be well adapted to both primary and secondary forest, and exhibit ecological plasticity. Statistically some loud call parameters vary among the putative species of the genus Phaner and not Euoticus. Phaner pallescens at Kirindy, Madasgascar was clearly able to discriminate loud calls of its own population from other proposed species within the genus. Most loud call parameters did not show any statistical degradation with increasing distance. A crucial finding of my study was that the two lineages are not only convergent in terms of dietary and locomotor aspects of their ecology, but also in the structures of their loud calls. The environmental adaptation of communication systems is often overlooked when studying adaptive convergence, but my study indicates that acoustic properties are a crucial aspect of a species’ preferred habitat. The results of this study can be used to improve conservation planning, and also add to our understanding of the evolutionary history of strepsirhine primates.
- Full Text:
- Date Issued: 2016
- Authors: Forbanka, Derick Nomuh
- Date: 2016
- Subjects: Adaptation (Biology) , Mammals -- Evolution , Mammals -- Behavior
- Language: English
- Type: Thesis , Doctoral , Zoology
- Identifier: http://hdl.handle.net/10353/15957 , vital:40567
- Description: The suborder Strepsirhini contains a large proportion of the living gummivorous mammal taxa, and includes two genera of specialist gummivores, Euoticus and Phaner, which present particularly interesting cases of convergent adaptive evolution in Africa and Madagascar, respectively. Although these genera are not closely related, they share several anatomical, behavioural and physiological specialisations associated with their diets, and despite their biogeographical separation, there seems to be substantial coincidence in their habitats and habits. Neither the ecology nor the systematics of Phaner and Euoticus has been the subject of much detailed research, and little is known of the conservation status of the genera. Even their specific diversity is unresolved. In this study I investigated and compared aspects of their ecology at both a macro-and a microhabitat scale, including wild populations of all groups within the two genera that have been accorded species status in recent field guides and systematic reviews. I also studied aspects of their social communication behaviour as clues to their species-level diversity. Species and their formation (or speciation) have inspired a great deal of evolutionary research, but remain contentious issues in evolutionary biology. This is due both to the difficulties inherent in inferring the details of a dynamic biological process that occurred over some time in the past from the recent, relatively static patterns of variation observed, as well as the limited consensus among biologists in identifying such patterns. It is therefore important to distinguish the kinds of patterns in nature that are predicted by different views of species and speciation, and also to see how well these predictions fare when applied to the speciation patterns found in strepsirhine primates. Theories of speciation cannot be separated from species concepts, and one concept widely supported in primatology is the Recognition Concept of species because of its heuristic nature. The Recognition Concept is based on Specific-mate Recognition Systems (SMRS). I focussed on SMRS features of Euoticus and Phaner, loud call specificity, and species’ preferred habitats. My results provided some support for the distinctiveness of currently identified species, although this was not clear-cut. Population densities of both Phaner and Euoticus appear to have declined in recent years, even though both Phaner and Euoticus appear to be well adapted to both primary and secondary forest, and exhibit ecological plasticity. Statistically some loud call parameters vary among the putative species of the genus Phaner and not Euoticus. Phaner pallescens at Kirindy, Madasgascar was clearly able to discriminate loud calls of its own population from other proposed species within the genus. Most loud call parameters did not show any statistical degradation with increasing distance. A crucial finding of my study was that the two lineages are not only convergent in terms of dietary and locomotor aspects of their ecology, but also in the structures of their loud calls. The environmental adaptation of communication systems is often overlooked when studying adaptive convergence, but my study indicates that acoustic properties are a crucial aspect of a species’ preferred habitat. The results of this study can be used to improve conservation planning, and also add to our understanding of the evolutionary history of strepsirhine primates.
- Full Text:
- Date Issued: 2016
Post-2008 voter apathy among the youth in the Eastern Cape : a comparative study of urban and rural municipalities
- Authors: Peter, Bongeka
- Date: 2016
- Subjects: Youth -- Political activity -- South Africa -- Eastern Cape Political participation -- South Africa -- Eastern Cape Apathy -- South Africa Municipal government -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , M Soc Sci
- Identifier: http://hdl.handle.net/10353/630 , vital:26475
- Description: This dissertation deals with the nature of the black middle-class assimilation in the South African suburban space, a space that was the sole preserve of the white middle-class during apartheid. It explores the relationship between these races as they come to meet in this space and what new identities are being formed. It also explores the relationship between both the black and white suburbanites and the urban poor who stay in an adjacent area to the suburb. The study uses the Beacon Bay area, which is constituted by one of East London’s most affluent suburbs and a poor township, Nompumelelo, to show how the emergent black middle-class has managed to enter this space in the post-apartheid era. Previous studies by Richard Ballard (2004) and Grant Saff (2001) have shown how the white middle-class has always been against any form of race or class mixing. Within the suburb, the new black suburbanites in Beacon Bay appear to have been welcomed but with conditions by their fellow white counterparts. The relationship between these two races does not stretch beyond meet and greets and it is only in the second generation black middle-class that you find better and non-superficial relations with fellow white suburbanites. In the older generation, the generation that experienced apartheid, the relationship between these two races has been that of tolerance and serious escape of contact unless when necessary. The children of both white and black families, though, have a far better relationship in school and in sport than their parents. This has created another area of contact for both these races and it bears potential for meaningful integration in the suburban space. Externally as it relates to relations between the black middle-class and the urban poor, the findings show that these new black suburbanites express a similar discomfort as the white suburbanites about the urban poor’s presence in the area. This shows that the evolution of the Beacon Bay suburb, with its deep-rooted discourse of white middle-class exclusivity, has not been entirely about hatred of the urban poor necessarily but about an identity ascription of what it means to live in a suburb. Despite these realities traditional ceremonies organised by the black middle-class in the suburbs and the church appear to be playing a role in creating relations between these suburbanites and the Nompumelelo residents. This is why we have decided to use the conceptualisation of the 18th century frontier zone as the borders of segregation within the suburb and between the suburban residents and those of the township can be crossed and re-crossed.
- Full Text:
- Date Issued: 2016
- Authors: Peter, Bongeka
- Date: 2016
- Subjects: Youth -- Political activity -- South Africa -- Eastern Cape Political participation -- South Africa -- Eastern Cape Apathy -- South Africa Municipal government -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , M Soc Sci
- Identifier: http://hdl.handle.net/10353/630 , vital:26475
- Description: This dissertation deals with the nature of the black middle-class assimilation in the South African suburban space, a space that was the sole preserve of the white middle-class during apartheid. It explores the relationship between these races as they come to meet in this space and what new identities are being formed. It also explores the relationship between both the black and white suburbanites and the urban poor who stay in an adjacent area to the suburb. The study uses the Beacon Bay area, which is constituted by one of East London’s most affluent suburbs and a poor township, Nompumelelo, to show how the emergent black middle-class has managed to enter this space in the post-apartheid era. Previous studies by Richard Ballard (2004) and Grant Saff (2001) have shown how the white middle-class has always been against any form of race or class mixing. Within the suburb, the new black suburbanites in Beacon Bay appear to have been welcomed but with conditions by their fellow white counterparts. The relationship between these two races does not stretch beyond meet and greets and it is only in the second generation black middle-class that you find better and non-superficial relations with fellow white suburbanites. In the older generation, the generation that experienced apartheid, the relationship between these two races has been that of tolerance and serious escape of contact unless when necessary. The children of both white and black families, though, have a far better relationship in school and in sport than their parents. This has created another area of contact for both these races and it bears potential for meaningful integration in the suburban space. Externally as it relates to relations between the black middle-class and the urban poor, the findings show that these new black suburbanites express a similar discomfort as the white suburbanites about the urban poor’s presence in the area. This shows that the evolution of the Beacon Bay suburb, with its deep-rooted discourse of white middle-class exclusivity, has not been entirely about hatred of the urban poor necessarily but about an identity ascription of what it means to live in a suburb. Despite these realities traditional ceremonies organised by the black middle-class in the suburbs and the church appear to be playing a role in creating relations between these suburbanites and the Nompumelelo residents. This is why we have decided to use the conceptualisation of the 18th century frontier zone as the borders of segregation within the suburb and between the suburban residents and those of the township can be crossed and re-crossed.
- Full Text:
- Date Issued: 2016
Preparation and application of plasmon metal enhanced titanium dioxide photocatalyst for the removal of organics in water
- Authors: Nyamukamba, Pardon
- Date: 2016
- Subjects: Water -- Purification -- Photocatalysis Titanium dioxide Water chemistry
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10353/2765 , vital:28074
- Description: Advanced oxidation processes are capable of removing organic compounds that cannot be removed by conventional water treatment methods. Among the oxidation processes, photo-catalysis using titanium dioxide (TiO2) is a promising method but suffers from rapid electron-hole recombination rates and only absorbs UV light which is a small percentage (5 percent) of the total solar radiation. Therefore there is a need to reduce the recombination rates and also extend the absorption of the photo-catalyst into the visible region which constitutes 55 percent of the total solar radiation. The major aims of this study were to prepare plasmon metal decorated and doped TiO2 photo-catalysts immobilized on quartz substrates and test their photo-catalytic and antimicrobial activities. The effect of film thickness (loading) and use of different shapes of plasmon metal nanostructures was investigated. TiO2 thin films were prepared by a sputter coating technique while plasmon metal (Au & Ag)/carbon co-doped TiO2 by a simple sol gel process and plasmon metal films were prepared by the thermal evaporation technique. Different plasmon metal nanostructures (nanorods, dendrites, nanowires and spherical nanoparticles) were prepared using a wet chemical technique using sodium borohydride as the reducing agent. Nanocomposites of co-doped TiO2 photo-catalyst and plasmon elements of different proportions were also prepared. The prepared photo-catalysts were coated onto etched and MPTMS (3-Mercaptopropyl trimethoxysliane) treated quartz glass substrate which is a stable support favouring easy recovery. The prepared materials were characterized by XRD, HRTEM, TEM, HRSEM, FT-IR, SEM, PIXE and TGA while the doped TiO2 was characterized by XPS, BET, CHNS and Raman Spectroscopy. The effect of pH of solution, presence of other contaminants and salts in solution, initial concentration of the model pollutant and type of the plasmonic elements on the photocatalytic activity of TiO2 towards 4-(4-sulfophenylazo)-N,N-dimethyl aniline (methyl orange) were also investigated. The selected TiO2 photo-catalyst films were tested for antimicrobial properties. The effect of different types of plasmon elements on the antimicrobial activity of TiO2 against E. coli ATCC 3695 was evaluated under both sunlight and weak UV light. Under UV light, Ag showed the highest enhancement in photo-catalytic activity of TiO2 than Au and Cu. The photo-catalytic activity of TiO2 increased with an increase in Ag content to an optimum loading and then started to decrease with a further increase in loading. For Cu and Au, photo-activity activity increased with an increase in plasmon metal content. Under sunlight, Cu showed the highest enhancement of TiO2 photocatalytic compared to Ag and Au. The change in order of deposition showed that Au films enhanced the photo-activity better when they were deposited underneath rather than on top of TiO2 on quartz supports but Ag films performed better in enhancing photo-activity when they were deposited on top of TiO2. The use of bimetallic layers and three layer systems of different plasmon elements enhanced photo-catalytic activity better than the use of a monometallic layer. The presence of other organic contaminants and salts in solutions was found to reduce the photo-degradation of methyl orange due to preferential adsorption of other contaminants. When the pH was increased, the photocatalytic activity of TiO2 towards methyl orange was reduced. In antimicrobial studies, it was found that the plasmon elements greatly improved the antibacterial action of TiO2 against Escherichia coli ATCC 3695 in water and the best antibacterial action was observed with silver/carbon co-doped TiO2 photo-catalyst under sunlight The doped samples consisted of polydisperse nanoparticles which were found to be beneficial for photo-catalytic activity enhancement under sunlight.
- Full Text:
- Date Issued: 2016
- Authors: Nyamukamba, Pardon
- Date: 2016
- Subjects: Water -- Purification -- Photocatalysis Titanium dioxide Water chemistry
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10353/2765 , vital:28074
- Description: Advanced oxidation processes are capable of removing organic compounds that cannot be removed by conventional water treatment methods. Among the oxidation processes, photo-catalysis using titanium dioxide (TiO2) is a promising method but suffers from rapid electron-hole recombination rates and only absorbs UV light which is a small percentage (5 percent) of the total solar radiation. Therefore there is a need to reduce the recombination rates and also extend the absorption of the photo-catalyst into the visible region which constitutes 55 percent of the total solar radiation. The major aims of this study were to prepare plasmon metal decorated and doped TiO2 photo-catalysts immobilized on quartz substrates and test their photo-catalytic and antimicrobial activities. The effect of film thickness (loading) and use of different shapes of plasmon metal nanostructures was investigated. TiO2 thin films were prepared by a sputter coating technique while plasmon metal (Au & Ag)/carbon co-doped TiO2 by a simple sol gel process and plasmon metal films were prepared by the thermal evaporation technique. Different plasmon metal nanostructures (nanorods, dendrites, nanowires and spherical nanoparticles) were prepared using a wet chemical technique using sodium borohydride as the reducing agent. Nanocomposites of co-doped TiO2 photo-catalyst and plasmon elements of different proportions were also prepared. The prepared photo-catalysts were coated onto etched and MPTMS (3-Mercaptopropyl trimethoxysliane) treated quartz glass substrate which is a stable support favouring easy recovery. The prepared materials were characterized by XRD, HRTEM, TEM, HRSEM, FT-IR, SEM, PIXE and TGA while the doped TiO2 was characterized by XPS, BET, CHNS and Raman Spectroscopy. The effect of pH of solution, presence of other contaminants and salts in solution, initial concentration of the model pollutant and type of the plasmonic elements on the photocatalytic activity of TiO2 towards 4-(4-sulfophenylazo)-N,N-dimethyl aniline (methyl orange) were also investigated. The selected TiO2 photo-catalyst films were tested for antimicrobial properties. The effect of different types of plasmon elements on the antimicrobial activity of TiO2 against E. coli ATCC 3695 was evaluated under both sunlight and weak UV light. Under UV light, Ag showed the highest enhancement in photo-catalytic activity of TiO2 than Au and Cu. The photo-catalytic activity of TiO2 increased with an increase in Ag content to an optimum loading and then started to decrease with a further increase in loading. For Cu and Au, photo-activity activity increased with an increase in plasmon metal content. Under sunlight, Cu showed the highest enhancement of TiO2 photocatalytic compared to Ag and Au. The change in order of deposition showed that Au films enhanced the photo-activity better when they were deposited underneath rather than on top of TiO2 on quartz supports but Ag films performed better in enhancing photo-activity when they were deposited on top of TiO2. The use of bimetallic layers and three layer systems of different plasmon elements enhanced photo-catalytic activity better than the use of a monometallic layer. The presence of other organic contaminants and salts in solutions was found to reduce the photo-degradation of methyl orange due to preferential adsorption of other contaminants. When the pH was increased, the photocatalytic activity of TiO2 towards methyl orange was reduced. In antimicrobial studies, it was found that the plasmon elements greatly improved the antibacterial action of TiO2 against Escherichia coli ATCC 3695 in water and the best antibacterial action was observed with silver/carbon co-doped TiO2 photo-catalyst under sunlight The doped samples consisted of polydisperse nanoparticles which were found to be beneficial for photo-catalytic activity enhancement under sunlight.
- Full Text:
- Date Issued: 2016
Production of Indigenous Leafy Vegetables (ILVs) and their contribution to household food security: evidence from Coffee Bay, Eastern Cape Province of South Africa
- Authors: Mayekiso Anele
- Date: 2016
- Subjects: Food security , Edible greens , Vegetable gardening -- South Africa , Food -- Preservation
- Language: English
- Type: Thesis , Masters , MSc Agric (Agricultural Economics)
- Identifier: http://hdl.handle.net/10353/712 , vital:26490 , Food security , Edible greens , Vegetable gardening -- South Africa , Food -- Preservation
- Description: This study used cross-sectional survey data to estimate farmers` perceptions of, and commonly cultivated ILVs, factors that influence the participation of smallholder famers in the production of Indigenous Leafy Vegetables and its contribution to household food security]. With regard to farmers` perceptions of ILVs, descriptive results reveal that a majority of the people from the study area share positive perceptions with respect to ILVs. Regression estimates for determinants of participation indicate that the production of ILVs is primarily conditioned by shared perceptions and institutional factors rather than the socio-economic attributes of farmers. Public policies that address the institutional framework (extension, credit, market and social networks) in favour of ILVs are more likely to promote production. Also, more research on the documentation and benefits of ILVs, supported by investments targeting educational campaigns towards promoting positive attitudes and dispelling fears and myths surrounding ILVs, will further promote production. With reference to the contribution of ILVs to food security, descriptive results indicate that participation in the production of ILVs leads to a higher HDDS and a lower HFIAS. Regression estimates further revealed that participation positively contributes to a higher HDDS and a lower HFIAS, suggesting that households who participate in the production of ILVs are more likely to be food secure than non-participants. Therefore, participation in the production of ILVs has significant potential to address household food security.
- Full Text:
- Date Issued: 2016
- Authors: Mayekiso Anele
- Date: 2016
- Subjects: Food security , Edible greens , Vegetable gardening -- South Africa , Food -- Preservation
- Language: English
- Type: Thesis , Masters , MSc Agric (Agricultural Economics)
- Identifier: http://hdl.handle.net/10353/712 , vital:26490 , Food security , Edible greens , Vegetable gardening -- South Africa , Food -- Preservation
- Description: This study used cross-sectional survey data to estimate farmers` perceptions of, and commonly cultivated ILVs, factors that influence the participation of smallholder famers in the production of Indigenous Leafy Vegetables and its contribution to household food security]. With regard to farmers` perceptions of ILVs, descriptive results reveal that a majority of the people from the study area share positive perceptions with respect to ILVs. Regression estimates for determinants of participation indicate that the production of ILVs is primarily conditioned by shared perceptions and institutional factors rather than the socio-economic attributes of farmers. Public policies that address the institutional framework (extension, credit, market and social networks) in favour of ILVs are more likely to promote production. Also, more research on the documentation and benefits of ILVs, supported by investments targeting educational campaigns towards promoting positive attitudes and dispelling fears and myths surrounding ILVs, will further promote production. With reference to the contribution of ILVs to food security, descriptive results indicate that participation in the production of ILVs leads to a higher HDDS and a lower HFIAS. Regression estimates further revealed that participation positively contributes to a higher HDDS and a lower HFIAS, suggesting that households who participate in the production of ILVs are more likely to be food secure than non-participants. Therefore, participation in the production of ILVs has significant potential to address household food security.
- Full Text:
- Date Issued: 2016