Financial liberalization and financial instability in the selected SADC member countries
- Authors: Cele, Nolungelo Mercy
- Date: 2018
- Subjects: Finance Financial crises Finance -- Developing countries
- Language: English
- Type: Thesis , Masters , MCom
- Identifier: http://hdl.handle.net/10353/8978 , vital:34179
- Description: The study examined the impact of financial liberalization on financial instability in selected SADC member countries namely South Africa, Tanzania, Madagascar and Botswana for the period 1970-2012. The Panel data methodology was adopted to establish the relationship between the two variables. Impaired loans were used to capture financial instability and financial reforms to capture the level of financial liberalization. Credit to the private sector, government expenditure, GDP and inflation were utilised as control variables The empirical findings reveal that financial liberalization leads to financial instability. The financial reforms were found to be positively related with the impaired loans ratio in almost all the specifications. It was also found that financial instability intensifies when the global financial crisis is taken into consideration. This suggests that financial liberalization can therefore be another source of financial instability in the SADC countries. The empirical results imply that policy makers should focus on reforms that give due share to the regulations rather than just simply liberalizing the financial sector.
- Full Text:
- Date Issued: 2018
- Authors: Cele, Nolungelo Mercy
- Date: 2018
- Subjects: Finance Financial crises Finance -- Developing countries
- Language: English
- Type: Thesis , Masters , MCom
- Identifier: http://hdl.handle.net/10353/8978 , vital:34179
- Description: The study examined the impact of financial liberalization on financial instability in selected SADC member countries namely South Africa, Tanzania, Madagascar and Botswana for the period 1970-2012. The Panel data methodology was adopted to establish the relationship between the two variables. Impaired loans were used to capture financial instability and financial reforms to capture the level of financial liberalization. Credit to the private sector, government expenditure, GDP and inflation were utilised as control variables The empirical findings reveal that financial liberalization leads to financial instability. The financial reforms were found to be positively related with the impaired loans ratio in almost all the specifications. It was also found that financial instability intensifies when the global financial crisis is taken into consideration. This suggests that financial liberalization can therefore be another source of financial instability in the SADC countries. The empirical results imply that policy makers should focus on reforms that give due share to the regulations rather than just simply liberalizing the financial sector.
- Full Text:
- Date Issued: 2018
Gender equity in land tenure : an assessment of the challenges faced by women in the communal land tenure system in Keiskammahoek, South Africa
- Authors: Sonkosi, Abongile
- Date: 2018
- Subjects: Land tenure--South Africa--Eastern Cape Land use--South Africa--Eastern Cape Land tenure
- Language: English
- Type: Thesis , Masters , Rural Development
- Identifier: http://hdl.handle.net/10353/17138 , vital:40859
- Description: Despite the importance of land to women, women however are still deprived of security of tenure as a result of the gender biased laws which at their best only protect married women and at their worst fail to protect the rights of single, widowed and divorced women. An analysis of the land reform policies in South Africa as a whole on the issue of the advancement of women through land reform reveals a number of irregularities and false promises. Many of the new policy shifts are problematic as they tend to benefit the elite and continue to leave the majority of the rural poor people particularly women in communal areas with insecure land tenure. This research study examined: 1. the nature of land relations at work in Keiskammahoek and how they impact women’s rights to land. 2. How women in Keiskammahoek view their rights to land. 3. The challenges experienced and progress made by women in claiming their land rights in Keiskamahoek. Despite the importance of land to women, women however are still deprived of security of tenure as a result of the gender biased laws which at their best only protect married women and at their worst fail to protect the rights of single, widowed and divorced women. An analysis of the land reform policies in South Africa as a whole on the issue of the advancement of women through land reform reveals a number of irregularities and false promises. Many of the new policy shifts are problematic as they tend to benefit the elite and continue to leave the majority of the rural poor people particularly women in communal areas with insecure land tenure. This research study examined: 1. the nature of land relations at work in Keiskammahoek and how they impact women’s rights to land. 2. How women in Keiskammahoek view their rights to land. 3. The challenges experienced and progress made by women in claiming their land rights in Keiskamahoek. ownership in their communities. They expressed strong views against the suppression of their voice due to patriarchal structures that govern the way they live. They further observed that a woman’s voice in the rural communities generally does not get heard and that even if women get into positions of power they fail to stand up against women’s marginalization and gender inequalities. However, the most positive finding is that the wind of change is blowing in Keiskammahoek as different categories of women - married, single, divorced, widowed etc. begin to access residential and to some limited degree, agricultural plots. They appeal to the government to support them in accessing agricultural tools including tractors, irrigation systems, seeds etc. Although the progress made so far with regard to women’s access to land is acknowledged, a great deal of work still needs to be done in order to empower women through land reform. The study makes recommendations on the implementation of land tenure policy that addresses women’s challenges in land access and ownership.
- Full Text:
- Date Issued: 2018
- Authors: Sonkosi, Abongile
- Date: 2018
- Subjects: Land tenure--South Africa--Eastern Cape Land use--South Africa--Eastern Cape Land tenure
- Language: English
- Type: Thesis , Masters , Rural Development
- Identifier: http://hdl.handle.net/10353/17138 , vital:40859
- Description: Despite the importance of land to women, women however are still deprived of security of tenure as a result of the gender biased laws which at their best only protect married women and at their worst fail to protect the rights of single, widowed and divorced women. An analysis of the land reform policies in South Africa as a whole on the issue of the advancement of women through land reform reveals a number of irregularities and false promises. Many of the new policy shifts are problematic as they tend to benefit the elite and continue to leave the majority of the rural poor people particularly women in communal areas with insecure land tenure. This research study examined: 1. the nature of land relations at work in Keiskammahoek and how they impact women’s rights to land. 2. How women in Keiskammahoek view their rights to land. 3. The challenges experienced and progress made by women in claiming their land rights in Keiskamahoek. Despite the importance of land to women, women however are still deprived of security of tenure as a result of the gender biased laws which at their best only protect married women and at their worst fail to protect the rights of single, widowed and divorced women. An analysis of the land reform policies in South Africa as a whole on the issue of the advancement of women through land reform reveals a number of irregularities and false promises. Many of the new policy shifts are problematic as they tend to benefit the elite and continue to leave the majority of the rural poor people particularly women in communal areas with insecure land tenure. This research study examined: 1. the nature of land relations at work in Keiskammahoek and how they impact women’s rights to land. 2. How women in Keiskammahoek view their rights to land. 3. The challenges experienced and progress made by women in claiming their land rights in Keiskamahoek. ownership in their communities. They expressed strong views against the suppression of their voice due to patriarchal structures that govern the way they live. They further observed that a woman’s voice in the rural communities generally does not get heard and that even if women get into positions of power they fail to stand up against women’s marginalization and gender inequalities. However, the most positive finding is that the wind of change is blowing in Keiskammahoek as different categories of women - married, single, divorced, widowed etc. begin to access residential and to some limited degree, agricultural plots. They appeal to the government to support them in accessing agricultural tools including tractors, irrigation systems, seeds etc. Although the progress made so far with regard to women’s access to land is acknowledged, a great deal of work still needs to be done in order to empower women through land reform. The study makes recommendations on the implementation of land tenure policy that addresses women’s challenges in land access and ownership.
- Full Text:
- Date Issued: 2018
Household's perceptions and determinants of participation in harvesting rangeland products :|bthe case of Dyamala community, Raymond Mhlaba Local Municipality, Eastern Cape Province, South Africa
- Authors: Mdiya, Lwandiso
- Date: 2018
- Subjects: Rangelands -- South Africa -- Eastern Cape Communal rangelands -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/9187 , vital:34303
- Description: The study reviews the household’s perceptions and determinants of sustainable products harvesting. Communal rangelands are vast natural landscapes in the form of grasslands, shrubs, woodlands, wetlands and deserts with multiple functions to the rural households. Most rural communities share boundaries and interact with rangelands for multiple socioeconomic and environmental reasons. Since most of these interactions are external to the price mechanism, the actual contribution of rangelands to communities has largely been missed. In an effort to appraise and explore the communal rangeland contribution to households, a study from Dyamala community in the Raymond Mhlaba Local Municipality under Amathole District in Eastern Cape was conducted using cross-sectional data. Descriptive results revealed that the majority of the respondents (71 percent) from the study area positively perceived communal rangelands as safety nets. however, there also emerged a fair share of challenges namely, (bad spirit, host predators, compete with arable land) leading to negative perceptions towards communal rangelands. It was also evident that, the majority of respondents from the study area use communal rangelands for fuel wood, construction poles, traditional medicine, with minor harvests of wild food (flora and fauna) and thatch grass. The results of the study also revealed that, rural people depend more on social grants for their income followed by incomes from remittances, agricultural activities and communal rangelands. However, it was established from the results that though communal rangelands provided commercial products capable of generating household income, rangeland users trading such products were receiving meagre incomes. This might be caused by the lack of institutional support to rural households thus, the low incomes received from communal rangelands. To that effect, main communal rangeland products such as, fuel wood, poles and medicine were cited as being more valuable for local domestic use than for the external market. Regression estimates further indicated that participation in communal rangeland products harvesting is more conditioned by perception-related factors as compared to socioeconomic attributes of rural households worth understanding for strategic targeting to promote their conservation. The extant literature and the study results, evidently show that communal rangelands provide a livelihood among rural households although it has a low direct household income potential. Therefore, the respondents were of the view that the use of campaigns targeting several perceptions (such as communal rangelands act as a source of medicinal plants, arable land and income etc) as suggested by the regression estimates may be very useful. The support from the extension department and further research may be necessary for purposes of quantifying harvestable rangeland products capable of enhancing rural livelihoods. Moreover, the observed low income potential of communal rangeland products suggests several technical and institutional challenges worth addressing to promote value addition of rangeland products.
- Full Text:
- Date Issued: 2018
- Authors: Mdiya, Lwandiso
- Date: 2018
- Subjects: Rangelands -- South Africa -- Eastern Cape Communal rangelands -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/9187 , vital:34303
- Description: The study reviews the household’s perceptions and determinants of sustainable products harvesting. Communal rangelands are vast natural landscapes in the form of grasslands, shrubs, woodlands, wetlands and deserts with multiple functions to the rural households. Most rural communities share boundaries and interact with rangelands for multiple socioeconomic and environmental reasons. Since most of these interactions are external to the price mechanism, the actual contribution of rangelands to communities has largely been missed. In an effort to appraise and explore the communal rangeland contribution to households, a study from Dyamala community in the Raymond Mhlaba Local Municipality under Amathole District in Eastern Cape was conducted using cross-sectional data. Descriptive results revealed that the majority of the respondents (71 percent) from the study area positively perceived communal rangelands as safety nets. however, there also emerged a fair share of challenges namely, (bad spirit, host predators, compete with arable land) leading to negative perceptions towards communal rangelands. It was also evident that, the majority of respondents from the study area use communal rangelands for fuel wood, construction poles, traditional medicine, with minor harvests of wild food (flora and fauna) and thatch grass. The results of the study also revealed that, rural people depend more on social grants for their income followed by incomes from remittances, agricultural activities and communal rangelands. However, it was established from the results that though communal rangelands provided commercial products capable of generating household income, rangeland users trading such products were receiving meagre incomes. This might be caused by the lack of institutional support to rural households thus, the low incomes received from communal rangelands. To that effect, main communal rangeland products such as, fuel wood, poles and medicine were cited as being more valuable for local domestic use than for the external market. Regression estimates further indicated that participation in communal rangeland products harvesting is more conditioned by perception-related factors as compared to socioeconomic attributes of rural households worth understanding for strategic targeting to promote their conservation. The extant literature and the study results, evidently show that communal rangelands provide a livelihood among rural households although it has a low direct household income potential. Therefore, the respondents were of the view that the use of campaigns targeting several perceptions (such as communal rangelands act as a source of medicinal plants, arable land and income etc) as suggested by the regression estimates may be very useful. The support from the extension department and further research may be necessary for purposes of quantifying harvestable rangeland products capable of enhancing rural livelihoods. Moreover, the observed low income potential of communal rangeland products suggests several technical and institutional challenges worth addressing to promote value addition of rangeland products.
- Full Text:
- Date Issued: 2018
Image processing and data analysis tools of a remote sensing-based euthrophication monitoring system
- Authors: Chamunorwa, Brighton
- Date: 2018
- Subjects: Eutrophication Image processing
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/10210 , vital:35376
- Description: Numerous surface water bodies in South Africa face serious challenges of eutrophication despite several initiatives from government to solve the problem. Government efforts have not been successful particularly because of three issues: 1) insufficient and inconsistent water quality data and 2) the costly nature of collecting this data. Third, the limited uptake of remote sensing based technologies in water quality management due to lack of skills, as well as the lengthy and complex procedures involved in retrieving water quality parameters. This thesis aims to explore the possibilities of using satellite data and simple software for mapping and collecting water quality data. The broad goal was to determine the function of software dedicated to meet the requirements of a remote sensing-based eutrophication-monitoring system. Two specific goal were set in this study: 1. To extract software requirements from available eutrophication management documentation. This was necessary to determine the functions of the dedicated software that matches the legislative requirements and 2. To determine a design capable of handling spatial and temporal requirement of a remote sensing based eutrophication-monitoring system. The study applied Goal Based Requirement Analysis Model [GBRAM] model to extract goals from the National Eutrophication Monitoring Programme [NEMP] implementation plan document and recent publication on remote sensing of water quality monitoring conducted in South Africa. Afterwards, the study used object-oriented concepts to model suitable data objects and processes to implement spatial-temporal requirements of a remote sensing-based eutrophication-monitoring system. Based on the study findings the following are essential functions of a remote sensing based eutrophication-monitoring system. A graphical user interface that allows the user to set up a monitoring programming. Automated image processing procedures. Data assessment methods used to generate eutrophication status information. Numerous display options for viewing data in several perfectives.
- Full Text:
- Date Issued: 2018
Image processing and data analysis tools of a remote sensing-based euthrophication monitoring system
- Authors: Chamunorwa, Brighton
- Date: 2018
- Subjects: Eutrophication Image processing
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/10210 , vital:35376
- Description: Numerous surface water bodies in South Africa face serious challenges of eutrophication despite several initiatives from government to solve the problem. Government efforts have not been successful particularly because of three issues: 1) insufficient and inconsistent water quality data and 2) the costly nature of collecting this data. Third, the limited uptake of remote sensing based technologies in water quality management due to lack of skills, as well as the lengthy and complex procedures involved in retrieving water quality parameters. This thesis aims to explore the possibilities of using satellite data and simple software for mapping and collecting water quality data. The broad goal was to determine the function of software dedicated to meet the requirements of a remote sensing-based eutrophication-monitoring system. Two specific goal were set in this study: 1. To extract software requirements from available eutrophication management documentation. This was necessary to determine the functions of the dedicated software that matches the legislative requirements and 2. To determine a design capable of handling spatial and temporal requirement of a remote sensing based eutrophication-monitoring system. The study applied Goal Based Requirement Analysis Model [GBRAM] model to extract goals from the National Eutrophication Monitoring Programme [NEMP] implementation plan document and recent publication on remote sensing of water quality monitoring conducted in South Africa. Afterwards, the study used object-oriented concepts to model suitable data objects and processes to implement spatial-temporal requirements of a remote sensing-based eutrophication-monitoring system. Based on the study findings the following are essential functions of a remote sensing based eutrophication-monitoring system. A graphical user interface that allows the user to set up a monitoring programming. Automated image processing procedures. Data assessment methods used to generate eutrophication status information. Numerous display options for viewing data in several perfectives.
- Full Text:
- Date Issued: 2018
Implementation of programmes for maintaining care and safety of learners in secondary schools in the Graaff Reinet education district
- Authors: Bilatyi, Nkosana Carlon
- Date: 2018
- Subjects: Schools -- Security measures -- Eastern Cape School environment -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10353/9379 , vital:34339
- Description: Care and safety of learners refers to the behavioral practices which protect children from danger, risks and injuries. It also includes environment that is free from violence since where there is no fear perceived, there is reverence for the school and its disciplinary measures (Hernandez & Seem, 2004; Marotz, Cross, Rush, 2005; Mathe, 2008; Chukwu, 2008). Schools should have positive environment which allows members of staff, learners and guests to freely interact without threats or fear. Learners and staff should feel psychologically, physically and emotionally safe and secure (Dwyer & Osher, 2000; Tabancali &Bektas, 2009; Butcher& Manning 2005). Consequently, care and safety of learners can also be viewed in a more practical and management oriented way. According to Hull (2010) care and safety of learners in a management oriented way includes, maintenance of school buildings and grounds, care of facilities, furniture and equipment with spacious corridors that allow free flow and emergency movement to prevent illness and death. Classes should accommodate the required educator-learner ratio. The playgrounds are reduced to limited space to ensure that learners are visible and are controllable. Moreover provision of care and safety of learners includes school culture and the appropriate training of educators as well as school staff who should respond to threats and hazards. It also includes addressing the range of crisis the school can face such as death and natural disasters (Phaneuf, 2009). Reeves, Nickerson, Conolly-Wilson, Suson, Lazzaro, Jimerson, and Pesce (2012) contend that the educators on duty should provide learners with safety precautions to guard against the possibility of single learner slipping away and suffering injuries (Dilion, 2007). According to Mogopodi, Banyaladzi & Petros (2015) facilities like laboratories should be suitable for all learners because they help to transform theoretical education into practice making learning effective. Astor, Guerra, Van Acker (2010) suggest that provision of care and safety for learners is among the most essential components for achieving effective teaching and learning. This will only be achieved by putting in place proper care and safety programmes and ensuring that they are effectively implemented (Netshitahame, & Vollenhoven, 2002, Astor et.al.2010).
- Full Text:
- Date Issued: 2018
- Authors: Bilatyi, Nkosana Carlon
- Date: 2018
- Subjects: Schools -- Security measures -- Eastern Cape School environment -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10353/9379 , vital:34339
- Description: Care and safety of learners refers to the behavioral practices which protect children from danger, risks and injuries. It also includes environment that is free from violence since where there is no fear perceived, there is reverence for the school and its disciplinary measures (Hernandez & Seem, 2004; Marotz, Cross, Rush, 2005; Mathe, 2008; Chukwu, 2008). Schools should have positive environment which allows members of staff, learners and guests to freely interact without threats or fear. Learners and staff should feel psychologically, physically and emotionally safe and secure (Dwyer & Osher, 2000; Tabancali &Bektas, 2009; Butcher& Manning 2005). Consequently, care and safety of learners can also be viewed in a more practical and management oriented way. According to Hull (2010) care and safety of learners in a management oriented way includes, maintenance of school buildings and grounds, care of facilities, furniture and equipment with spacious corridors that allow free flow and emergency movement to prevent illness and death. Classes should accommodate the required educator-learner ratio. The playgrounds are reduced to limited space to ensure that learners are visible and are controllable. Moreover provision of care and safety of learners includes school culture and the appropriate training of educators as well as school staff who should respond to threats and hazards. It also includes addressing the range of crisis the school can face such as death and natural disasters (Phaneuf, 2009). Reeves, Nickerson, Conolly-Wilson, Suson, Lazzaro, Jimerson, and Pesce (2012) contend that the educators on duty should provide learners with safety precautions to guard against the possibility of single learner slipping away and suffering injuries (Dilion, 2007). According to Mogopodi, Banyaladzi & Petros (2015) facilities like laboratories should be suitable for all learners because they help to transform theoretical education into practice making learning effective. Astor, Guerra, Van Acker (2010) suggest that provision of care and safety for learners is among the most essential components for achieving effective teaching and learning. This will only be achieved by putting in place proper care and safety programmes and ensuring that they are effectively implemented (Netshitahame, & Vollenhoven, 2002, Astor et.al.2010).
- Full Text:
- Date Issued: 2018
Implementation of strategies used to manage conflict between students and staff in secondary schools in Malawi : towards a comprehensive framework
- Authors: Zimpita, Valentino Tipitana
- Date: 2018
- Subjects: Conflict management Conflict management -- Study and teaching Violence -- Prevention
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10353/9412 , vital:34348
- Description: The study aimed at examining how strategies used to manage conflict between students and staff in secondary schools in Malawi were implemented. This was after it had been noted that conflicts in secondary schools in Malawi usually ended in violence. The study employed a pragmatic research paradigm. In this paradigm, a researcher employs mixed methods approach which collects both quantitative and qualitative data. Using a concurrent triangulation mixed method design, the two types of data were collected concurrently and analysed simultaneously. Study participants were head teachers, teachers, students, education officials and parents. Most of the teachers were randomly sampled while a few other teachers, the head teachers, the education officials and the parents were purposefully sampled. The study found that there were various causes of conflict in secondary schools in Malawi which included misunderstanding of human rights, poor communication between students and staff, poor school management by head teachers and lack of students‘ involvement in decisions that concerned them. The study further found that conflict was in a way beneficial to schools as it was a learning point for both students and staff. However, such benefits were overshadowed by the many evils that violence brought such as disturbing teaching and learning. The study also found that there were two approaches in the way schools managed conflict; engagement of students and calling for police intervention. It transpired that on the overall, stakeholders were aware of different conflict management skills but they did not know how best to use them. In addition, it transpired that stakeholders were not comfortable with win-win approaches to conflict management. This was against a background that the stakeholders were involved in conflict management in different ways. It also came out clear that support to secondary schools in the management of conflict was at two levels; school level and system level. On the part of monitoring, it transpired that the mechanism which were there were ad hoc and they included inspection visits and the requirement that head teachers should always submit reports which could carry issues related to conflict. The study concluded that poorly managed conflicts which turned into violence were a menace to secondary schools in Malawi. In this regard, there was need to ensure effective conflict management in order to avoid violence. To achieve this, the study has made a number of recommendations. Among them, it has been recommended that schools should from time to time take stork of the likely causes of conflict and address them accordingly. It has also been recommended that schools should put in place mechanisms for ensuring two way communication so that students feel free to air out their concerns. At Ministry level, it has been recommended that the MoEST should take guidance and counselling seriously by among other things training teacher councillors and making the post an established one. It has also been recommended that the MoEST should make sure that head teachers, deputy head teachers, teachers, parents and even education officials are inducted in conflict management.
- Full Text:
- Date Issued: 2018
- Authors: Zimpita, Valentino Tipitana
- Date: 2018
- Subjects: Conflict management Conflict management -- Study and teaching Violence -- Prevention
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10353/9412 , vital:34348
- Description: The study aimed at examining how strategies used to manage conflict between students and staff in secondary schools in Malawi were implemented. This was after it had been noted that conflicts in secondary schools in Malawi usually ended in violence. The study employed a pragmatic research paradigm. In this paradigm, a researcher employs mixed methods approach which collects both quantitative and qualitative data. Using a concurrent triangulation mixed method design, the two types of data were collected concurrently and analysed simultaneously. Study participants were head teachers, teachers, students, education officials and parents. Most of the teachers were randomly sampled while a few other teachers, the head teachers, the education officials and the parents were purposefully sampled. The study found that there were various causes of conflict in secondary schools in Malawi which included misunderstanding of human rights, poor communication between students and staff, poor school management by head teachers and lack of students‘ involvement in decisions that concerned them. The study further found that conflict was in a way beneficial to schools as it was a learning point for both students and staff. However, such benefits were overshadowed by the many evils that violence brought such as disturbing teaching and learning. The study also found that there were two approaches in the way schools managed conflict; engagement of students and calling for police intervention. It transpired that on the overall, stakeholders were aware of different conflict management skills but they did not know how best to use them. In addition, it transpired that stakeholders were not comfortable with win-win approaches to conflict management. This was against a background that the stakeholders were involved in conflict management in different ways. It also came out clear that support to secondary schools in the management of conflict was at two levels; school level and system level. On the part of monitoring, it transpired that the mechanism which were there were ad hoc and they included inspection visits and the requirement that head teachers should always submit reports which could carry issues related to conflict. The study concluded that poorly managed conflicts which turned into violence were a menace to secondary schools in Malawi. In this regard, there was need to ensure effective conflict management in order to avoid violence. To achieve this, the study has made a number of recommendations. Among them, it has been recommended that schools should from time to time take stork of the likely causes of conflict and address them accordingly. It has also been recommended that schools should put in place mechanisms for ensuring two way communication so that students feel free to air out their concerns. At Ministry level, it has been recommended that the MoEST should take guidance and counselling seriously by among other things training teacher councillors and making the post an established one. It has also been recommended that the MoEST should make sure that head teachers, deputy head teachers, teachers, parents and even education officials are inducted in conflict management.
- Full Text:
- Date Issued: 2018
Implementation of the school nutrition programme in selected high schools in KwaZulu-Natal Province : implications on learners' nutrition education
- Authors: Mafugu, Tafirenyika
- Date: 2018
- Subjects: School children -- Nutrition -- KwaZulu-Natal School children -- Food -- South Africa -- KwaZulu-Natal Child welfare -- South Africa -- KwaZulu-Natal
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10353/9637 , vital:34812
- Description: The study sought to: examine training, monitoring and support provided to NSNP stakeholders; establish what informs the procurement and preparation of the food; determine its nutritional value; identify challenges encountered in the implementation of the school nutritrition programme; and evaluate the implications of the NSNP on learners’ nutritional education in Pinetown district in South Africa. Underpinned by the pragmatic research paradigm, the study followed a mixed methods research approach which utilised a convergent parallel research design. A random sample of 108 learners and purposive samples of 32 different stakeholders participated in the study. Descriptive and inferential statistics were used to analyse quantitative data, while qualitative data were categorised into themes which were presented in tables and text. The two forms of data were merged at the interpretation phase through triangulation of results. The study found that some of the key stakeholders of NSNP were not adequately supported and trained, and that monitoring was only done regularly by the teacher coordinators. District field officers lacked adequate expertise to train stakeholders. Food was prepared following a menu which was not updated in some schools, and food handlers in two schools could not understand quantities on the menu. The suppliers used the menu for procurement of food. The observed mean intakes per child per day for energy, protein, total fat, cholesterol, dietary fibre, vitamin K and sodium were significantly lower than the expected mean nutrient intakes, while the intakes of all other nutrients were not significantly different statistically, from the expected nutrient intakes. The intakes of all nutrients were below the minimum of 30 percent of RDA which was recommended by the Department of Basic Education. Fruits were rarely supplied. Nutritional education was not adequately taught across the curriculum and most respondents were not aware of basic nutritional principles. Training stakeholders and early payment of the suppliers could significantly improve learners’ benefit from the programme. The study’s proposed framework of implementation of the school nutrition programme recommends ways to improve the implementation process, including the incorporation of nutrition education into the Life Orientation curriculum to enhance good food choices that curb prevalent nutrient disorders among South Africans.
- Full Text:
- Date Issued: 2018
- Authors: Mafugu, Tafirenyika
- Date: 2018
- Subjects: School children -- Nutrition -- KwaZulu-Natal School children -- Food -- South Africa -- KwaZulu-Natal Child welfare -- South Africa -- KwaZulu-Natal
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10353/9637 , vital:34812
- Description: The study sought to: examine training, monitoring and support provided to NSNP stakeholders; establish what informs the procurement and preparation of the food; determine its nutritional value; identify challenges encountered in the implementation of the school nutritrition programme; and evaluate the implications of the NSNP on learners’ nutritional education in Pinetown district in South Africa. Underpinned by the pragmatic research paradigm, the study followed a mixed methods research approach which utilised a convergent parallel research design. A random sample of 108 learners and purposive samples of 32 different stakeholders participated in the study. Descriptive and inferential statistics were used to analyse quantitative data, while qualitative data were categorised into themes which were presented in tables and text. The two forms of data were merged at the interpretation phase through triangulation of results. The study found that some of the key stakeholders of NSNP were not adequately supported and trained, and that monitoring was only done regularly by the teacher coordinators. District field officers lacked adequate expertise to train stakeholders. Food was prepared following a menu which was not updated in some schools, and food handlers in two schools could not understand quantities on the menu. The suppliers used the menu for procurement of food. The observed mean intakes per child per day for energy, protein, total fat, cholesterol, dietary fibre, vitamin K and sodium were significantly lower than the expected mean nutrient intakes, while the intakes of all other nutrients were not significantly different statistically, from the expected nutrient intakes. The intakes of all nutrients were below the minimum of 30 percent of RDA which was recommended by the Department of Basic Education. Fruits were rarely supplied. Nutritional education was not adequately taught across the curriculum and most respondents were not aware of basic nutritional principles. Training stakeholders and early payment of the suppliers could significantly improve learners’ benefit from the programme. The study’s proposed framework of implementation of the school nutrition programme recommends ways to improve the implementation process, including the incorporation of nutrition education into the Life Orientation curriculum to enhance good food choices that curb prevalent nutrient disorders among South Africans.
- Full Text:
- Date Issued: 2018
Implementing values education as a vehicle to enhance moral regeneration among youths in secondary schools in Gokwe South District, Zimbabwe: towards a holistic framework
- Authors: Ndondo, Shepherd
- Date: 2018
- Subjects: Values -- Study and teaching -- Zimbabwe Moral education
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10353/9423 , vital:34350
- Description: This study focused on the implementation of values education strategies in secondary schools in Gokwe South District in Zimbabwe. The study employed the interpretive paradigm and it used the qualitative approach and case study research design. Purposive sampling was used to select 34 participants who were rich informants and these included 1 District Schools Inspector, 3 School heads, 3 School counsellors, 3 Religious Studies teachers, 3 History teachers, 3 Arts and Culture teachers and 18 learners (6 from each school). Data were obtained through face to face interviews, focus groups and document analysis. The study revealed that most learners in these schools embodied ubuntu that shaped their behaviour. ln order to deal with elements of indiscipline, the schools adopted values education strategies which included Positive discipline, Guidance and Counselling, teaching of Religious Studies, Citizenship Education, teaching of Arts and Culture, and Parental Involvement which were implemented in the selected schools in different ways. Imparting values based knowledge to students is a commendable step, however, there were gaps that were noted in the implementation process. Some subjects like Citizenship Education existed on paper but not in practice in some schools, as students claimed ignorance of their existence. Although these subjects were supervised and supported by the District Schools Inspector (DSI) and the School heads, lack of funds and limited resources negatively affected their implementation. With regard to discipline procedures, it was noted that some teachers utilized corporal punishment, despite it being outlawed in the country, as stated in the Ministry of Education Sports and Culture Circular P.35 of 1999. It emerged that there was lack of qualified School counsellors, Arts and Culture teachers and inadequate Parental Involvement in values disciplines. It was also established that lack of financial resources constrained the implementation of values education strategies in that teachers could not go for staff development workshops. Notwithstanding the hindrances encountered, the study found that there were pockets of good practices in implementing values education. The study concluded that selected secondary schools used several strategies in values education which involved counselling misbehaving learners and involving parents when their children have behaviour problems. It was also revealed that members of the school community were playing different roles in the implementation process. The study recommended that there should be active participation of all members in positive disciplinary procedures so that values education strategies become effective.The study also recommended that teachers be trained in teaching values disciplines for effective implementation. Lastly, based on the findings of the study and literature, the researcher proposed an alternative model for implementing values education strategies that will hopefully contribute to the eradication of moral decadence among the youths.
- Full Text:
- Date Issued: 2018
- Authors: Ndondo, Shepherd
- Date: 2018
- Subjects: Values -- Study and teaching -- Zimbabwe Moral education
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10353/9423 , vital:34350
- Description: This study focused on the implementation of values education strategies in secondary schools in Gokwe South District in Zimbabwe. The study employed the interpretive paradigm and it used the qualitative approach and case study research design. Purposive sampling was used to select 34 participants who were rich informants and these included 1 District Schools Inspector, 3 School heads, 3 School counsellors, 3 Religious Studies teachers, 3 History teachers, 3 Arts and Culture teachers and 18 learners (6 from each school). Data were obtained through face to face interviews, focus groups and document analysis. The study revealed that most learners in these schools embodied ubuntu that shaped their behaviour. ln order to deal with elements of indiscipline, the schools adopted values education strategies which included Positive discipline, Guidance and Counselling, teaching of Religious Studies, Citizenship Education, teaching of Arts and Culture, and Parental Involvement which were implemented in the selected schools in different ways. Imparting values based knowledge to students is a commendable step, however, there were gaps that were noted in the implementation process. Some subjects like Citizenship Education existed on paper but not in practice in some schools, as students claimed ignorance of their existence. Although these subjects were supervised and supported by the District Schools Inspector (DSI) and the School heads, lack of funds and limited resources negatively affected their implementation. With regard to discipline procedures, it was noted that some teachers utilized corporal punishment, despite it being outlawed in the country, as stated in the Ministry of Education Sports and Culture Circular P.35 of 1999. It emerged that there was lack of qualified School counsellors, Arts and Culture teachers and inadequate Parental Involvement in values disciplines. It was also established that lack of financial resources constrained the implementation of values education strategies in that teachers could not go for staff development workshops. Notwithstanding the hindrances encountered, the study found that there were pockets of good practices in implementing values education. The study concluded that selected secondary schools used several strategies in values education which involved counselling misbehaving learners and involving parents when their children have behaviour problems. It was also revealed that members of the school community were playing different roles in the implementation process. The study recommended that there should be active participation of all members in positive disciplinary procedures so that values education strategies become effective.The study also recommended that teachers be trained in teaching values disciplines for effective implementation. Lastly, based on the findings of the study and literature, the researcher proposed an alternative model for implementing values education strategies that will hopefully contribute to the eradication of moral decadence among the youths.
- Full Text:
- Date Issued: 2018
In silico analysis, isolation and kinetic characterisation of red algae (Rhodophyta) catalases
- Authors: Nodangala, Sinovuyo
- Date: 2018
- Subjects: Red algae Marine algae
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/14626 , vital:40024
- Description: Catalase (EC: 1.11.1.6) is produced by almost all aerobic organism ranging from bacteria to human and is an essential antioxidant enzyme that catalyses the conversion of hydrogen peroxide to water and molecular oxygen, therefore neutralising hydrogen peroxide toxicity. The present study aimed to purify and characterise catalase from Pachymenia orbitosa, a marine red algae found in the Algoa Bay region of South Africa. Bioinformatics analysis was performed to confirm the presence of a catalase gene in the red algae genome. In silico analysis of the Chondrus crispus genome was performed to predict the functional and structural characterisation of the protein encoded by the gene. The Pachymenia orbitosa catalase enzyme was purified to homogeneity using 60% ammonium sulphate precipitation and Sephacryl S-200 gel filtration chromatography. InterProScan confirmed that the Chondrus crispus genome encodes a catalase protein, which is from the mono-functional and heme-dependent catalase-like superfamily. The purified Pachymenia orbitosa catalase enzyme displayed a specific activity of 23 000 units per mg of protein with a 14.94% recovery and 222.91 fold purification. Sodium dodecyl sulphatepolyacrylamide gel electrophoresis indicated a single band, with a molecular weight of around 50kDa. The catalase enzyme exhibited a maximum activity at pH 7 and a temperature of 30℃. It was stable up to 40℃ and rapidly denatured at temperatures above this. The Km and Vmax values for the purified catalase, using hydrogen peroxide as a substrate, were determined from the Lineweaver-Burk plot to be 22.22mM and 1.11x10-4mM.min-1 , respectively, while from the Hanes-Woolf plot, to be 23.4mM and 1.17x10-4mM.min-1 , respectively. The Heme catalase inhibitor (ferricyanide) inhibited the enzyme activity markedly, while sodium chloride and citric acid had only a slight inhibitory effect. Copper sulphate showed a slight stimulatory effect. The physiochemical properties suggest a good application potential in both the pharmaceutical and food industries
- Full Text:
- Date Issued: 2018
- Authors: Nodangala, Sinovuyo
- Date: 2018
- Subjects: Red algae Marine algae
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/14626 , vital:40024
- Description: Catalase (EC: 1.11.1.6) is produced by almost all aerobic organism ranging from bacteria to human and is an essential antioxidant enzyme that catalyses the conversion of hydrogen peroxide to water and molecular oxygen, therefore neutralising hydrogen peroxide toxicity. The present study aimed to purify and characterise catalase from Pachymenia orbitosa, a marine red algae found in the Algoa Bay region of South Africa. Bioinformatics analysis was performed to confirm the presence of a catalase gene in the red algae genome. In silico analysis of the Chondrus crispus genome was performed to predict the functional and structural characterisation of the protein encoded by the gene. The Pachymenia orbitosa catalase enzyme was purified to homogeneity using 60% ammonium sulphate precipitation and Sephacryl S-200 gel filtration chromatography. InterProScan confirmed that the Chondrus crispus genome encodes a catalase protein, which is from the mono-functional and heme-dependent catalase-like superfamily. The purified Pachymenia orbitosa catalase enzyme displayed a specific activity of 23 000 units per mg of protein with a 14.94% recovery and 222.91 fold purification. Sodium dodecyl sulphatepolyacrylamide gel electrophoresis indicated a single band, with a molecular weight of around 50kDa. The catalase enzyme exhibited a maximum activity at pH 7 and a temperature of 30℃. It was stable up to 40℃ and rapidly denatured at temperatures above this. The Km and Vmax values for the purified catalase, using hydrogen peroxide as a substrate, were determined from the Lineweaver-Burk plot to be 22.22mM and 1.11x10-4mM.min-1 , respectively, while from the Hanes-Woolf plot, to be 23.4mM and 1.17x10-4mM.min-1 , respectively. The Heme catalase inhibitor (ferricyanide) inhibited the enzyme activity markedly, while sodium chloride and citric acid had only a slight inhibitory effect. Copper sulphate showed a slight stimulatory effect. The physiochemical properties suggest a good application potential in both the pharmaceutical and food industries
- Full Text:
- Date Issued: 2018
Incidence and antibiogram fingerprints of members of the Enterobacteriaceae family recovered from river water, hospital effluents and vegetables in Chris Hani and Amathole District Municipalities in the Eastern Cape Province
- Authors: Mpaka, Lindelwa
- Date: 2018
- Subjects: Enterobacteriaceae
- Language: English
- Type: Thesis , Masters , MSc (Microbiology)
- Identifier: http://hdl.handle.net/10353/14549 , vital:40003
- Description: The worldwide problem of antimicrobial resistance has limited the spectrum of the current affordable and effective antimicrobials. Infections associated with resistant microorganisms impose a major threat to public health and economic stability. Globally, about 700 000 deaths every year can be accredited to antimicrobial resistance. The leading mechanism of resistance amid bacterial pathogens is the extended spectrum beta-lactamases production, which inhibits spectrum activity of several antimicrobial agents. The rise in antimicrobial resistance has compelled an urgent need of developing means of combatting resistance issue amid diseasecausing microbes. The main aim of this study is to evaluate the incidence and antibiogram fingerprints of Enterobacteriaceae recovered from hospital effluents, river water and vegetables in the Eastern Cape Province. A total of eighteen antibiotics from ten different antimicrobial classes were used to determine antibiogram profiles of the MALDI-TOF confirmed isolates. From the MALDI-TOF confirmed isolates, 60% of Enterobacter spp. and E. coli isolates displayed resistance against colistin, while Citrobacter spp. and Klebsiella spp. displayed 90% and 60% resistance against this antimicrobial respectively. These findings outline the need for the development of new antimicrobials. About 75.5% (25/33) of the presumptive Enterobacter spp. were confirmed by MALDI-TOF with 79.2% (19/24), 66.7% (2/3), 66.7% (4/6) been confirmed vegetables, hospital effluents and river water samples respectively. Likewise, about 77.8% (21/27) were confirmed as Citrobacter spp. of which 92.3% (12/13), 66.7% (2/3) and 63.6% (7/11) were from vegetables, hospital effluents and river water samples respectively. These results show that the selected vegetables were highly contaminated with resistant bacteria and thus unsafe to consume uncooked vegetable. Also river water was higly contaminated with resistant microbes, which also shows that these rivers are not fit to be used 17 as drinking water sources and recreational activities. Colistin is an antimicrobial used as a last resort of antibiotics because it exhibits broad-spectrum activity. However from the findings of the work at present, this is no longer the case. The spectrum of this antimicrobial is now reduced by Enterobacteriaceae members. To the best of my knowledge; relatively few resources have been provided to understanding, preventing, and controlling increasing antimicrobial resistance on global, national and local levels.
- Full Text:
- Date Issued: 2018
- Authors: Mpaka, Lindelwa
- Date: 2018
- Subjects: Enterobacteriaceae
- Language: English
- Type: Thesis , Masters , MSc (Microbiology)
- Identifier: http://hdl.handle.net/10353/14549 , vital:40003
- Description: The worldwide problem of antimicrobial resistance has limited the spectrum of the current affordable and effective antimicrobials. Infections associated with resistant microorganisms impose a major threat to public health and economic stability. Globally, about 700 000 deaths every year can be accredited to antimicrobial resistance. The leading mechanism of resistance amid bacterial pathogens is the extended spectrum beta-lactamases production, which inhibits spectrum activity of several antimicrobial agents. The rise in antimicrobial resistance has compelled an urgent need of developing means of combatting resistance issue amid diseasecausing microbes. The main aim of this study is to evaluate the incidence and antibiogram fingerprints of Enterobacteriaceae recovered from hospital effluents, river water and vegetables in the Eastern Cape Province. A total of eighteen antibiotics from ten different antimicrobial classes were used to determine antibiogram profiles of the MALDI-TOF confirmed isolates. From the MALDI-TOF confirmed isolates, 60% of Enterobacter spp. and E. coli isolates displayed resistance against colistin, while Citrobacter spp. and Klebsiella spp. displayed 90% and 60% resistance against this antimicrobial respectively. These findings outline the need for the development of new antimicrobials. About 75.5% (25/33) of the presumptive Enterobacter spp. were confirmed by MALDI-TOF with 79.2% (19/24), 66.7% (2/3), 66.7% (4/6) been confirmed vegetables, hospital effluents and river water samples respectively. Likewise, about 77.8% (21/27) were confirmed as Citrobacter spp. of which 92.3% (12/13), 66.7% (2/3) and 63.6% (7/11) were from vegetables, hospital effluents and river water samples respectively. These results show that the selected vegetables were highly contaminated with resistant bacteria and thus unsafe to consume uncooked vegetable. Also river water was higly contaminated with resistant microbes, which also shows that these rivers are not fit to be used 17 as drinking water sources and recreational activities. Colistin is an antimicrobial used as a last resort of antibiotics because it exhibits broad-spectrum activity. However from the findings of the work at present, this is no longer the case. The spectrum of this antimicrobial is now reduced by Enterobacteriaceae members. To the best of my knowledge; relatively few resources have been provided to understanding, preventing, and controlling increasing antimicrobial resistance on global, national and local levels.
- Full Text:
- Date Issued: 2018
Incidence of porcine circovirus type 2 and porcine parvoviruses in swine herds of some communities in Eastern Cape, South Africa
- Authors: Afolabi, Kayode Olayinka
- Date: 2018
- Subjects: Swine -- Diseases Swine -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10353/9691 , vital:34820
- Description: Porcine circovirus type 2 (PCV2) is one of the swine pathogens of global economic importance. Since its first detection in early 1990s as the main etiologic agent of porcine multisystemic wasting syndrome (PMWS) and many other porcine circovirus-associated diseases (PCVAD), the virus has been extensively studied and has been found to be present in virtually all the pig producing countries of the world. As a viral pathogen that brings about clinical diseases aided by co-infecting pathogens, the involvement of many other viral agents including porcine parvoviruses (PPVs) have caught the attention of stakeholders worldwide. However, no surveillance study of the viral pathogens has been carried out in South Africa as there are little or no information on their prevalence in the swine herds of the country. This present study therefore aimed at detection and molecular characterization of PCV2 and PPVs in swine herds of some selected communities in Eastern Cape Province, South Africa. A total of 375 field samples were collected from seven commercial and communal farms from three District Municipalities of Eastern Cape, South Africa between 2015 and 2016. Structured questionnaires were also administered to each farm at the time of sample collection to obtain some important information relating to health status and farm management practices in the sampled farms. With the aid of conventional PCR method, 339 samples were initially screened for the presence of PCV2; positive amplicons were sequenced and obtained partial genomes of the virus were preliminarily analyzed. In order to obtain the complete genomes of the virus, four overlapping primer pairs were used to amplify the full-genome of PCV2 from the initial positive samples; amplified genomes were sequenced using the Sanger methods, sequenced PCV2 genomes were assembled and characterized. Furthermore, the prevalences of some designated PPVs in the sampled farms were obtained using 110 samples randomly selected from the previously archived samples and screened with 6 different primer pairs specific for the detection of 7 PPVs. All the amplified parvoviruses’ genomes were sequenced; their sequenced partial genomes were subsequently base-culled and analysed. The data obtained revealed that 54/339 (15.93 percent) samples from the swine herds were positive for PCV2; whereas the degree of occurrence of the viral pathogen as observed at farm level ranges from approximately 5.6 to 60 percent. The majority 15/17 (88 percent) of the analyzed partial sequences were found clustering with other PCV2b strains in the phylogenetic analysis. More interestingly, two other sequences obtained were also found clustering within PCV2d genotype in the initial screening and analysis. Furthermore, 15 complete PCV2 genomes were successfully amplified, sequenced and assembled. NJ and ML phylogenetic analysis of the complete ORF2 gene and full genomes unanimously showed 11 of the assembled genomes belonging to genotype PCV2b. Another 3 of the characterized sequences formed clade with other reference mutant PCV2b and PCV2b subtype 1C (PCV2d) strains from different parts of the world. The last sequence however, clustered with other reference strains belonging to PCV2 intermediate clade 2 (PCV2-IM2) recently identified in a global PCV2 strains phylogenetic analysis. Other genetic analyses including multiple sequence alignment and p-distance analysis also confirmed the outcomes of the phylogenetic analyses of the complete capsid gene and fullgenomes of the virus. On the other hand, the findings of the molecular profiling for PPVs showed that all the screened parvoviruses were present in the study area, having prevalence of 29.1 percent (PPV1), 21.8 percent (PPV2), 5.5 percent (PPV3), 43.6 percent (PPV4), 21.8 percent (PBo-likeV) and 44.6 percent for PBoV1 and PBoV2. Double infection of the screened PPVs was observed to be very rampant among the pigs as high as 20/110 (18.2 percent) for PPV2/PPV4 and PPV4/PBoV; followed by 19/110 (17.3 percent) of the samples for PPV1/PPV4 and PPV1/PBoV. Three of the viruses were found simultaneously in 19 of the screened samples representing 17.3 percent, whereas 8 (7.3 percent) samples were positive for four of the viruses. Phylogenetic analyses of PPV1, PPV2 and PBoVs 1 and 2 were conducted with two major clades homologous for each of them. This is the first report of PCV2 in swine herds of the Province and the first detection of PCV2b, PCV2d and PCV2-IM2 strains in South African swine herds. It follows the first reported case of PCV2a in an outbreak of porcine multisystemic wasting syndrome (PMWS) in Gauteng Province, South Africa over two decades ago. Also, this is the first major epidemiologic study on PPVs in the country following the initial case study of 1975. These findings confirmed the presence of the allimportant viral pathogens among pigs and also give preliminary insights into the possibility of co-infections of the pathogens in the studied area. This could however result in a serious large scale outbreak of devastating disease(s) associated with the viral pathogens, thereby ultimately resulting in huge economic losses if no appropriate measures are taken to effectively curb their spread across the country.
- Full Text:
- Date Issued: 2018
- Authors: Afolabi, Kayode Olayinka
- Date: 2018
- Subjects: Swine -- Diseases Swine -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10353/9691 , vital:34820
- Description: Porcine circovirus type 2 (PCV2) is one of the swine pathogens of global economic importance. Since its first detection in early 1990s as the main etiologic agent of porcine multisystemic wasting syndrome (PMWS) and many other porcine circovirus-associated diseases (PCVAD), the virus has been extensively studied and has been found to be present in virtually all the pig producing countries of the world. As a viral pathogen that brings about clinical diseases aided by co-infecting pathogens, the involvement of many other viral agents including porcine parvoviruses (PPVs) have caught the attention of stakeholders worldwide. However, no surveillance study of the viral pathogens has been carried out in South Africa as there are little or no information on their prevalence in the swine herds of the country. This present study therefore aimed at detection and molecular characterization of PCV2 and PPVs in swine herds of some selected communities in Eastern Cape Province, South Africa. A total of 375 field samples were collected from seven commercial and communal farms from three District Municipalities of Eastern Cape, South Africa between 2015 and 2016. Structured questionnaires were also administered to each farm at the time of sample collection to obtain some important information relating to health status and farm management practices in the sampled farms. With the aid of conventional PCR method, 339 samples were initially screened for the presence of PCV2; positive amplicons were sequenced and obtained partial genomes of the virus were preliminarily analyzed. In order to obtain the complete genomes of the virus, four overlapping primer pairs were used to amplify the full-genome of PCV2 from the initial positive samples; amplified genomes were sequenced using the Sanger methods, sequenced PCV2 genomes were assembled and characterized. Furthermore, the prevalences of some designated PPVs in the sampled farms were obtained using 110 samples randomly selected from the previously archived samples and screened with 6 different primer pairs specific for the detection of 7 PPVs. All the amplified parvoviruses’ genomes were sequenced; their sequenced partial genomes were subsequently base-culled and analysed. The data obtained revealed that 54/339 (15.93 percent) samples from the swine herds were positive for PCV2; whereas the degree of occurrence of the viral pathogen as observed at farm level ranges from approximately 5.6 to 60 percent. The majority 15/17 (88 percent) of the analyzed partial sequences were found clustering with other PCV2b strains in the phylogenetic analysis. More interestingly, two other sequences obtained were also found clustering within PCV2d genotype in the initial screening and analysis. Furthermore, 15 complete PCV2 genomes were successfully amplified, sequenced and assembled. NJ and ML phylogenetic analysis of the complete ORF2 gene and full genomes unanimously showed 11 of the assembled genomes belonging to genotype PCV2b. Another 3 of the characterized sequences formed clade with other reference mutant PCV2b and PCV2b subtype 1C (PCV2d) strains from different parts of the world. The last sequence however, clustered with other reference strains belonging to PCV2 intermediate clade 2 (PCV2-IM2) recently identified in a global PCV2 strains phylogenetic analysis. Other genetic analyses including multiple sequence alignment and p-distance analysis also confirmed the outcomes of the phylogenetic analyses of the complete capsid gene and fullgenomes of the virus. On the other hand, the findings of the molecular profiling for PPVs showed that all the screened parvoviruses were present in the study area, having prevalence of 29.1 percent (PPV1), 21.8 percent (PPV2), 5.5 percent (PPV3), 43.6 percent (PPV4), 21.8 percent (PBo-likeV) and 44.6 percent for PBoV1 and PBoV2. Double infection of the screened PPVs was observed to be very rampant among the pigs as high as 20/110 (18.2 percent) for PPV2/PPV4 and PPV4/PBoV; followed by 19/110 (17.3 percent) of the samples for PPV1/PPV4 and PPV1/PBoV. Three of the viruses were found simultaneously in 19 of the screened samples representing 17.3 percent, whereas 8 (7.3 percent) samples were positive for four of the viruses. Phylogenetic analyses of PPV1, PPV2 and PBoVs 1 and 2 were conducted with two major clades homologous for each of them. This is the first report of PCV2 in swine herds of the Province and the first detection of PCV2b, PCV2d and PCV2-IM2 strains in South African swine herds. It follows the first reported case of PCV2a in an outbreak of porcine multisystemic wasting syndrome (PMWS) in Gauteng Province, South Africa over two decades ago. Also, this is the first major epidemiologic study on PPVs in the country following the initial case study of 1975. These findings confirmed the presence of the allimportant viral pathogens among pigs and also give preliminary insights into the possibility of co-infections of the pathogens in the studied area. This could however result in a serious large scale outbreak of devastating disease(s) associated with the viral pathogens, thereby ultimately resulting in huge economic losses if no appropriate measures are taken to effectively curb their spread across the country.
- Full Text:
- Date Issued: 2018
Influence of family structure on food security status of farming households in Alice, South Africa
- Authors: Ijila, Olusegun Jeremiah
- Date: 2018
- Subjects: Food security Families--South Africa--Eastern Cape Farms, Small
- Language: English
- Type: Thesis , Masters , Agricultural Economics
- Identifier: http://hdl.handle.net/10353/11537 , vital:39081
- Description: Achieving food security in sub-Saharan Africa remains a major challenge despite efforts made by a majority of the countries to reduce abject poverty and food insecurity. Just as these countries prioritized food security under the Millennium Development Goals (MDGs), they are equally putting it on top of the agenda under the Sustainable Development Goals (SDGs). In the face of the political and economic developments seen in the country since 1994, South Africa is overwhelmed by poverty and unemployment. But efforts to address these problems have not given due recognition to the question of family structure which is complex and varied, with immense potential implications for ownership and distribution of resources and the bargaining strength that are likely to determine the food security status of various farming households. Furthermore, the contribution of households towards agricultural production differs according to the way family organizes itself. This study was carried out to determine the influence of family structures on food security status of farming households in Alice, South Africa. Data were collected from 120 farming households’ selected using purposive sampling method. To collect data, a well-structured questionnaire was administered through face-to-face interviews. Consequent to that, the data were analyzed using descriptive statistics, and binary logistic regression model. The dominant family structures are nuclear, single-parent, working parent and cohabiting. The analysis revealed that nuclear family where father, mother and their children have access to labour and financial resources could be more comfortable than single parent family. Working parents would probably be more food secure because both parents are able to combine incomes while food insecurity might exist within cohabiting families since the union is less stable because of scarce resources occasioned by their inability to combine their resources to pursue a common goal. The results further revealed that single parent family structures and socio-economic characteristics like age, marital status and year of education of the household head were significant in terms of their influence on food security. The study therefore, recommends that old people should be encouraged to participate in agricultural production due to the wealth of experience gathered over the years. It is probably safe to conclude that encouraging marriage would provide better opportunities to raise the necessary capital to support positive productivity changes that would have implications for enhanced food availability and affordability.
- Full Text:
- Date Issued: 2018
- Authors: Ijila, Olusegun Jeremiah
- Date: 2018
- Subjects: Food security Families--South Africa--Eastern Cape Farms, Small
- Language: English
- Type: Thesis , Masters , Agricultural Economics
- Identifier: http://hdl.handle.net/10353/11537 , vital:39081
- Description: Achieving food security in sub-Saharan Africa remains a major challenge despite efforts made by a majority of the countries to reduce abject poverty and food insecurity. Just as these countries prioritized food security under the Millennium Development Goals (MDGs), they are equally putting it on top of the agenda under the Sustainable Development Goals (SDGs). In the face of the political and economic developments seen in the country since 1994, South Africa is overwhelmed by poverty and unemployment. But efforts to address these problems have not given due recognition to the question of family structure which is complex and varied, with immense potential implications for ownership and distribution of resources and the bargaining strength that are likely to determine the food security status of various farming households. Furthermore, the contribution of households towards agricultural production differs according to the way family organizes itself. This study was carried out to determine the influence of family structures on food security status of farming households in Alice, South Africa. Data were collected from 120 farming households’ selected using purposive sampling method. To collect data, a well-structured questionnaire was administered through face-to-face interviews. Consequent to that, the data were analyzed using descriptive statistics, and binary logistic regression model. The dominant family structures are nuclear, single-parent, working parent and cohabiting. The analysis revealed that nuclear family where father, mother and their children have access to labour and financial resources could be more comfortable than single parent family. Working parents would probably be more food secure because both parents are able to combine incomes while food insecurity might exist within cohabiting families since the union is less stable because of scarce resources occasioned by their inability to combine their resources to pursue a common goal. The results further revealed that single parent family structures and socio-economic characteristics like age, marital status and year of education of the household head were significant in terms of their influence on food security. The study therefore, recommends that old people should be encouraged to participate in agricultural production due to the wealth of experience gathered over the years. It is probably safe to conclude that encouraging marriage would provide better opportunities to raise the necessary capital to support positive productivity changes that would have implications for enhanced food availability and affordability.
- Full Text:
- Date Issued: 2018
Influence of trans-border trading activities on senior secondary school students' academic performance in economics in Ogun State, Nigeria
- Authors: Titus, Adesegun Benedict
- Date: 2018
- Subjects: Smuggling -- Education (Secondary) Borderlands -- Nigeria
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10353/9659 , vital:34814
- Description: The study investigated the influence of transborder trading activities on senior secondary school students’ academic performance in economics in Ogun state, Nigeria. A descriptive survey research design involving 320 students that comprised males and females were selected through stratified random sampling technique. Two self-developed instruments were used: student questionnaire on transborder trading activities (SQTTA) with a Cronbach alpha index of 0.7845; and the economics achievement test (EAT) with a co-efficient index of r= 0.814. The results of the data collected were collated, coded, analyzed and interpreted through the use of descriptive research statistics of frequency, mean, percentages, and standard deviation while Step-wise regression analysis was used to test the hypotheses raised in the study, and the inferential statistics t-test was used to test the significant differences between the independent and dependent variables. The results revealed that there is a positive significant influence of smuggling on students’ academic performance in economics concepts (t=4.123; df =307, p<0.05) and a negative significant influence of school location on students’ academic performance in economics concepts (t=0.952; df=307, p>0.05). The study revealed that the most prevalent transborder trading activities like smuggling of used tyres, small firearms and fairly used clothes had negative influence on students’ academic performance. Based on these findings, discussions were focused on the involvement of students in trans-border trading activities and the location of schools, amongst others, which has no significant influence on students’ academic performance. However, it was recommended that schools should not be located near border areas in future.
- Full Text:
- Date Issued: 2018
- Authors: Titus, Adesegun Benedict
- Date: 2018
- Subjects: Smuggling -- Education (Secondary) Borderlands -- Nigeria
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10353/9659 , vital:34814
- Description: The study investigated the influence of transborder trading activities on senior secondary school students’ academic performance in economics in Ogun state, Nigeria. A descriptive survey research design involving 320 students that comprised males and females were selected through stratified random sampling technique. Two self-developed instruments were used: student questionnaire on transborder trading activities (SQTTA) with a Cronbach alpha index of 0.7845; and the economics achievement test (EAT) with a co-efficient index of r= 0.814. The results of the data collected were collated, coded, analyzed and interpreted through the use of descriptive research statistics of frequency, mean, percentages, and standard deviation while Step-wise regression analysis was used to test the hypotheses raised in the study, and the inferential statistics t-test was used to test the significant differences between the independent and dependent variables. The results revealed that there is a positive significant influence of smuggling on students’ academic performance in economics concepts (t=4.123; df =307, p<0.05) and a negative significant influence of school location on students’ academic performance in economics concepts (t=0.952; df=307, p>0.05). The study revealed that the most prevalent transborder trading activities like smuggling of used tyres, small firearms and fairly used clothes had negative influence on students’ academic performance. Based on these findings, discussions were focused on the involvement of students in trans-border trading activities and the location of schools, amongst others, which has no significant influence on students’ academic performance. However, it was recommended that schools should not be located near border areas in future.
- Full Text:
- Date Issued: 2018
Inmate incarceration and rehabilitation : a cross sectional study of rehabilitation programmes and facilities in correctional centres in North Western Nigeria
- Authors: Amali, Sadiq Ewaoda
- Date: 2018
- Subjects: Criminal -- Rehabilitation Prisoners -- Counseling of Prisoners -- Mental health services
- Language: English
- Type: Thesis , Doctoral , Social Sciences
- Identifier: http://hdl.handle.net/10353/9984 , vital:35215
- Description: The study was conducted in Kano Central and Goron-Dutse prisons, North Western, Nigeria to determine the effectiveness of these correctional centres with regards to inmate rehabilitation. Both quantitative and qualitative collection was conducted concurrently and incorporated in the explanation of the results. This was achieved as the study empirically examined the experiences of convicted inmates and correctional officials in both correctional centres with a sample that compromised of both convicted male and female inmates as well as male and female correctional officials. A total of 250 questionnaires were administered to convicted inmates in Kano central and Goron-Dutse prisons. A sample size of 250 was determined using the sample size calculator at a confidence level of 95 percent, confidence interval of +/-5.97 percent, and with a population of 3488. Data for this study was generated from 250 respondents. Out of 250 respondents, questionnaires were administered to 140 inmates in Kano Central Prison and 110 questionnaires were administered to inmates in Goron Dutse Prison. The number of respondents in Kano Central Prisons is higher than that of Goron-Dutse Prison due to disparity in population of inmates. Also, 207 questionnaires were administered to correctional officials in Kano Central and Goron-Dutse prisons. A sample size of 207 was determined using the sample size calculator at a confidence interval of +/-5, confidence level of 95 percent and population of 449 correctional officials. Out of the 207 respondents, 107 questionnaires were administered to correctional officials in Kano Central Prison and 100 to correctional officials in Goron-Dutse Prison. The number of respondents in Kano Central Prison is higher than that of Goron-Dutse prison due to disparity in population of correctional officials. The selections were done using purposive sampling procedures. Rank, involvement in rehabilitation programmes were also used as criteria for selection of correctional officials as respondents. Furthermore, 28 correctional officials were selected purposively across the two correctional centres were interviewed. Thus, 14 correctional officials each were selected in Kano Central and Goron Dutse prisons using purposive sampling method. Thus, a total of 28 in-depth interviews were conducted in the study area. This study empirically probed into the factors mitigating against the effective rehabilitation of inmates. Therefore, against this background, this study involved an appraisal of rehabilitation programmes and facilities which was followed by an evaluation of inmates’ opinion of the correctional centre social setting and overall practices. Thus, inmate’s perceptions of correctional officials’ attitudes towards them and also its implication for re-integration principle was thoroughly appraised. The findings of this study were analyzed with the assistance of computer applications such as SPSS and atlas.ti.6. Some findings from this study revealed that only 15.7 percent of inmates in Kano Central prisons were satisfied with rehabilitation programmes, 37.3 percent were satisfied in Goron-Dutse prison while the majority of the inmates (88 percent) in Goron-Dutse prison stated that the relationship with correctional officials was cordial compared to 53.5 percent of the inmates in Kano Central prison. Also, data revealed that 64.2 percent of the inmates in Goron-Dutse Prison stated that medical attention was good and very good. The results also reveals that (58.9 percent) correctional officials stated that their relationship with inmates was very cordial compared to those of Goron-Dutse Prison (41.1 percent).Pearson Chi-square statistics showed that there was no statistical difference in correctional officials’ perceptions of the relationship with inmates between the two correctional centres (p.value>0.05). This study recommends that it is imperative for the Nigerian government to make more efforts with the provision of adequate rehabilitation programmes and facilities in the two correctional centres and correctional centres across the country as an exploration of existing literature reveals that most correctional centres in Nigeria lacked basic facilities for inmate rehabilitation as inmates were idle during incarceration.
- Full Text:
- Date Issued: 2018
- Authors: Amali, Sadiq Ewaoda
- Date: 2018
- Subjects: Criminal -- Rehabilitation Prisoners -- Counseling of Prisoners -- Mental health services
- Language: English
- Type: Thesis , Doctoral , Social Sciences
- Identifier: http://hdl.handle.net/10353/9984 , vital:35215
- Description: The study was conducted in Kano Central and Goron-Dutse prisons, North Western, Nigeria to determine the effectiveness of these correctional centres with regards to inmate rehabilitation. Both quantitative and qualitative collection was conducted concurrently and incorporated in the explanation of the results. This was achieved as the study empirically examined the experiences of convicted inmates and correctional officials in both correctional centres with a sample that compromised of both convicted male and female inmates as well as male and female correctional officials. A total of 250 questionnaires were administered to convicted inmates in Kano central and Goron-Dutse prisons. A sample size of 250 was determined using the sample size calculator at a confidence level of 95 percent, confidence interval of +/-5.97 percent, and with a population of 3488. Data for this study was generated from 250 respondents. Out of 250 respondents, questionnaires were administered to 140 inmates in Kano Central Prison and 110 questionnaires were administered to inmates in Goron Dutse Prison. The number of respondents in Kano Central Prisons is higher than that of Goron-Dutse Prison due to disparity in population of inmates. Also, 207 questionnaires were administered to correctional officials in Kano Central and Goron-Dutse prisons. A sample size of 207 was determined using the sample size calculator at a confidence interval of +/-5, confidence level of 95 percent and population of 449 correctional officials. Out of the 207 respondents, 107 questionnaires were administered to correctional officials in Kano Central Prison and 100 to correctional officials in Goron-Dutse Prison. The number of respondents in Kano Central Prison is higher than that of Goron-Dutse prison due to disparity in population of correctional officials. The selections were done using purposive sampling procedures. Rank, involvement in rehabilitation programmes were also used as criteria for selection of correctional officials as respondents. Furthermore, 28 correctional officials were selected purposively across the two correctional centres were interviewed. Thus, 14 correctional officials each were selected in Kano Central and Goron Dutse prisons using purposive sampling method. Thus, a total of 28 in-depth interviews were conducted in the study area. This study empirically probed into the factors mitigating against the effective rehabilitation of inmates. Therefore, against this background, this study involved an appraisal of rehabilitation programmes and facilities which was followed by an evaluation of inmates’ opinion of the correctional centre social setting and overall practices. Thus, inmate’s perceptions of correctional officials’ attitudes towards them and also its implication for re-integration principle was thoroughly appraised. The findings of this study were analyzed with the assistance of computer applications such as SPSS and atlas.ti.6. Some findings from this study revealed that only 15.7 percent of inmates in Kano Central prisons were satisfied with rehabilitation programmes, 37.3 percent were satisfied in Goron-Dutse prison while the majority of the inmates (88 percent) in Goron-Dutse prison stated that the relationship with correctional officials was cordial compared to 53.5 percent of the inmates in Kano Central prison. Also, data revealed that 64.2 percent of the inmates in Goron-Dutse Prison stated that medical attention was good and very good. The results also reveals that (58.9 percent) correctional officials stated that their relationship with inmates was very cordial compared to those of Goron-Dutse Prison (41.1 percent).Pearson Chi-square statistics showed that there was no statistical difference in correctional officials’ perceptions of the relationship with inmates between the two correctional centres (p.value>0.05). This study recommends that it is imperative for the Nigerian government to make more efforts with the provision of adequate rehabilitation programmes and facilities in the two correctional centres and correctional centres across the country as an exploration of existing literature reveals that most correctional centres in Nigeria lacked basic facilities for inmate rehabilitation as inmates were idle during incarceration.
- Full Text:
- Date Issued: 2018
Institutions, Production and Marketing of maize in Zimbabwe. Case of Marondera District of Mashonaland East Province
- Authors: Mafuse, Never
- Date: 2018
- Subjects: Food security Food supply
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10353/15687 , vital:40506
- Description: Food security situation in Zimbabwe is deteriorating despite the efforts made by the government for embarking on agrarian reforms in terms of redressing colonial imbalances and taking on the major trading investment risks. The government assured the resettled farmers that if they invest in maize production, they would not be stranded by lack of marketing services as the markets are liberalized. However, this market liberalization policy occurring in maize sector has resulted in A1 resettled farmers becoming more vulnerable to traders preying on their weaknesses as the policy is not living up to expectations and is failing to bring dramatic increases in agricultural growth. This may mean that sending children to school is a nightmare for those who depend on farming for educating their children, inadequate food in the home, unpaid loans and subsequent impoverishment. The resettled A1farmers will remain trapped in the poverty cycle as long as institutions are not corrected. The general aim of the study was to evaluate institutional factors influencing maize production and marketing by A1 resettled farmers of Marondera district in Mashonaland East province of Zimbabwe. Using a sample of 343 farmers randomly selected in Marondera District, different econometric models like Data envelopment Analysis, Cob Douglas, Binary logistic, and Tobit as well as descriptive statistics were used to analyse the data. Institutional variables were captured in two ways: as a dummy showing whether farmers have access to institutional variables and as a continuous variable indicating importance of institutions on maize production and marketing. The study found out that, most of the A1 farmers are old, with a mean age of 47 years and a maximum of 55 years of farming experience. It is also dominated by male farmers who are literate. Results also showed that most of A1 farmers from the sample are married and very few separated. These farmers sell most their maize and keep little for consumption though they are still using convectional farming methods. Most of the farmers from the sample generate their incomes from farming activities only and they have good access to market and production information as well as extension xvi messages through training .They however have poor access to financial credit and weak social capital as well as low contract farming. Results also indicated that A1 farmers pursue the formal supply chains than informal supply chains. It is noted that, A1 farmers participate in a number of markets that include agricultural inputs markets seed, fertilizers, chemicals.; credit markets which includes banks as well as labour markets which can in the form of (maricho). In terms production and marketing efficiency, the study found out that A1 farmers are technically, allocatively and economically inefficient. The maximum efficient score is 1 while the minimum score is 0.01 for both production and marketing stage. The mean scores for production stage are 0.195, 0.504 and 0.072 for technical, allocative and economic efficient respectively. The mean scores for marketing are 0.103, 0.688, and 0.068 for technical, allocative and economic efficiency respectively. Farmers are performing better at production stage than at marketing technically while there is better allocative and economic efficiency at marketing stage. Results for determinants of technical, allocative and economic efficiency at production stage showed that, cattle ownership, farming experience, access to financial credit and (member of farmer organisation) which was used as a proxy indicator for social capital ,access to information, contract farming, distance to input market ,area planted to maize and quantity of seed used influences marketing efficiency of A1 maize farmers in Marondera District. Access to credit, Access to market, distance to market, road infrastructure, farming experience and access to information influences technical, allocative and economic efficiency at marketing stage. Cooperative membership, road infrastructure, contractual farming arrangements, and access to financial credit and transport cost to market influence choice of marketing channel respectively. Finally results from the non linear cob Douglas reveals that, maize production is significantly influenced by contract with value chain actors, access to market xvii information, access to financial credit, total topdressing used , total basal fertilizer used, total herbicides used, and total area put under maize production. The results showed that, future of A1 resettled farmers depends on correcting the institutions which are of paramount importance for maize production and marketing. The study therefore recommended the government to improve institutional environment that has direct link with institutional innovations necessary for sustainable maize production and marketing. This can be done through correcting inefficiencies and ineffective institutions by creating and maintaining both environment and arrangement that will transform maize production and marketing systems to operate efficiently. The study presents policy options to address both production and marketing of maize arguing that new direction of production and marketing of maize depends on correcting dysfunctional institutions. Policy options include increasing A1 farmers access to financial credit by removing the stringent collateral requirements which are not attainable by most A1 farmers as their asset endowment is limited to non capital. Addressing imperfections in the input markets by improving the supply of chemical fertilisers and seed at affordable prices as this influences total area put under maize production. The other policy is for private companies and NGOs to offer contract farming to A1 maize farmers as this will help them to increase total area put under maize production, access to market, access to credit, and access to information and reduces long distances to purchase inputs. Introducing focused training to motivate resettled A1 farmers on formation farmer organisations as this assists in social capital which is an important asset in maize production and marketing.Improving road infrastructure by creating a fund that would be used to maintain the road rather waiting for DDF only.
- Full Text:
- Date Issued: 2018
- Authors: Mafuse, Never
- Date: 2018
- Subjects: Food security Food supply
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10353/15687 , vital:40506
- Description: Food security situation in Zimbabwe is deteriorating despite the efforts made by the government for embarking on agrarian reforms in terms of redressing colonial imbalances and taking on the major trading investment risks. The government assured the resettled farmers that if they invest in maize production, they would not be stranded by lack of marketing services as the markets are liberalized. However, this market liberalization policy occurring in maize sector has resulted in A1 resettled farmers becoming more vulnerable to traders preying on their weaknesses as the policy is not living up to expectations and is failing to bring dramatic increases in agricultural growth. This may mean that sending children to school is a nightmare for those who depend on farming for educating their children, inadequate food in the home, unpaid loans and subsequent impoverishment. The resettled A1farmers will remain trapped in the poverty cycle as long as institutions are not corrected. The general aim of the study was to evaluate institutional factors influencing maize production and marketing by A1 resettled farmers of Marondera district in Mashonaland East province of Zimbabwe. Using a sample of 343 farmers randomly selected in Marondera District, different econometric models like Data envelopment Analysis, Cob Douglas, Binary logistic, and Tobit as well as descriptive statistics were used to analyse the data. Institutional variables were captured in two ways: as a dummy showing whether farmers have access to institutional variables and as a continuous variable indicating importance of institutions on maize production and marketing. The study found out that, most of the A1 farmers are old, with a mean age of 47 years and a maximum of 55 years of farming experience. It is also dominated by male farmers who are literate. Results also showed that most of A1 farmers from the sample are married and very few separated. These farmers sell most their maize and keep little for consumption though they are still using convectional farming methods. Most of the farmers from the sample generate their incomes from farming activities only and they have good access to market and production information as well as extension xvi messages through training .They however have poor access to financial credit and weak social capital as well as low contract farming. Results also indicated that A1 farmers pursue the formal supply chains than informal supply chains. It is noted that, A1 farmers participate in a number of markets that include agricultural inputs markets seed, fertilizers, chemicals.; credit markets which includes banks as well as labour markets which can in the form of (maricho). In terms production and marketing efficiency, the study found out that A1 farmers are technically, allocatively and economically inefficient. The maximum efficient score is 1 while the minimum score is 0.01 for both production and marketing stage. The mean scores for production stage are 0.195, 0.504 and 0.072 for technical, allocative and economic efficient respectively. The mean scores for marketing are 0.103, 0.688, and 0.068 for technical, allocative and economic efficiency respectively. Farmers are performing better at production stage than at marketing technically while there is better allocative and economic efficiency at marketing stage. Results for determinants of technical, allocative and economic efficiency at production stage showed that, cattle ownership, farming experience, access to financial credit and (member of farmer organisation) which was used as a proxy indicator for social capital ,access to information, contract farming, distance to input market ,area planted to maize and quantity of seed used influences marketing efficiency of A1 maize farmers in Marondera District. Access to credit, Access to market, distance to market, road infrastructure, farming experience and access to information influences technical, allocative and economic efficiency at marketing stage. Cooperative membership, road infrastructure, contractual farming arrangements, and access to financial credit and transport cost to market influence choice of marketing channel respectively. Finally results from the non linear cob Douglas reveals that, maize production is significantly influenced by contract with value chain actors, access to market xvii information, access to financial credit, total topdressing used , total basal fertilizer used, total herbicides used, and total area put under maize production. The results showed that, future of A1 resettled farmers depends on correcting the institutions which are of paramount importance for maize production and marketing. The study therefore recommended the government to improve institutional environment that has direct link with institutional innovations necessary for sustainable maize production and marketing. This can be done through correcting inefficiencies and ineffective institutions by creating and maintaining both environment and arrangement that will transform maize production and marketing systems to operate efficiently. The study presents policy options to address both production and marketing of maize arguing that new direction of production and marketing of maize depends on correcting dysfunctional institutions. Policy options include increasing A1 farmers access to financial credit by removing the stringent collateral requirements which are not attainable by most A1 farmers as their asset endowment is limited to non capital. Addressing imperfections in the input markets by improving the supply of chemical fertilisers and seed at affordable prices as this influences total area put under maize production. The other policy is for private companies and NGOs to offer contract farming to A1 maize farmers as this will help them to increase total area put under maize production, access to market, access to credit, and access to information and reduces long distances to purchase inputs. Introducing focused training to motivate resettled A1 farmers on formation farmer organisations as this assists in social capital which is an important asset in maize production and marketing.Improving road infrastructure by creating a fund that would be used to maintain the road rather waiting for DDF only.
- Full Text:
- Date Issued: 2018
Investigation of strategies employed in rural households to improve food security in Raymond Mhlaba Local Municipality : a case of Zihlahleni Location in Middledrift, Eastern Cape Province
- Authors: Gqozo, Odwa
- Date: 2018
- Subjects: Food security -- South Africa -- Eastern Cape Rural development -- South Africa -- Eastern Cape Households -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/10758 , vital:35752
- Description: Eastern Cape is one of the provinces experiencing a rapid increase in food prices while household income is increasing at a slower rate. Against this background, the main objective of this study was to investigate strategies used by rural households to improve food security in Raymond Mhlaba Local Municipality formally known as Nkonkobe in the Eastern Cape Province, South Africa. Data were collected from 100 households using a questionnaire as the main instrument by using simple random sampling. Data were analyzed by employing simple descriptive statistics to describe household food security. Results revealed that most (50.3percent) households that were interviewed were male headed and unemployment was high (73.6percent). Moreover, the average age of the interviewed household heads was 59 years. Most (68percent) of the households owned home gardens and had access to arable land which was not being cultivated. Most (87percent) households indicated that lack of capital was the main cause of food security and depended on government grants. Most (100percent) of the interviewed household heads depend mostly on food purchases rather than their own production. The recommendation is that the government should continue fostering its agenda of improving agriculture as a strategy in rural households for curbing food insecurity.
- Full Text:
- Date Issued: 2018
- Authors: Gqozo, Odwa
- Date: 2018
- Subjects: Food security -- South Africa -- Eastern Cape Rural development -- South Africa -- Eastern Cape Households -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/10758 , vital:35752
- Description: Eastern Cape is one of the provinces experiencing a rapid increase in food prices while household income is increasing at a slower rate. Against this background, the main objective of this study was to investigate strategies used by rural households to improve food security in Raymond Mhlaba Local Municipality formally known as Nkonkobe in the Eastern Cape Province, South Africa. Data were collected from 100 households using a questionnaire as the main instrument by using simple random sampling. Data were analyzed by employing simple descriptive statistics to describe household food security. Results revealed that most (50.3percent) households that were interviewed were male headed and unemployment was high (73.6percent). Moreover, the average age of the interviewed household heads was 59 years. Most (68percent) of the households owned home gardens and had access to arable land which was not being cultivated. Most (87percent) households indicated that lack of capital was the main cause of food security and depended on government grants. Most (100percent) of the interviewed household heads depend mostly on food purchases rather than their own production. The recommendation is that the government should continue fostering its agenda of improving agriculture as a strategy in rural households for curbing food insecurity.
- Full Text:
- Date Issued: 2018
Investigation of the subsurface geology using time domain, magnetic and electrical resistivity geophysical techniques in the Karoo basin at Beaufort west, Western Cape Province, South Africa
- Authors: Ntunja, Asanda
- Date: 2018
- Subjects: Geology Geophysics
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/14659 , vital:40034
- Description: Beaufort West lies in the Central Karoo Basin and is specifically situated in the Poortjie Member of the Teekloof Formation in the Beaufort Group. The Teekloof Formation was deposited in a floodplain environment and it consists of alternating bluish grey mudstone and very fine to medium grained sandstone. The sediments of the Karoo Basin were intruded by numerous dolerite sills and dykes during a period of extensive magmatic activity over the whole South African subcontinent when the break-up of Gondwanda occurred. This led to a continuous jointing of the sedimentary rocks and the jointing resulted in rocks that are prone to weathering. Weathering that occurred in Beaufort West resulted in the formation of alluvium, calcrete, scree and hard pan deposits which overlay most of the ground surface. The joints in rocks paved way for groundwater storage in the sedimentary beds as sediments of the Karoo Basin lack significant primary porosity. Geophysical surveys were done in order to determine a suitable site to locate and drill a deep borehole to the White Hill Formation in order to extract shale gas that is proposed to be present in the area, thereafter determine sites for monitoring boreholes for groundwater and also look for area where potential groundwater might be present. This then requires geophysical investigations of the surface and subsurface geology of the area prior to drilling so as to locate any features such as dolerite intrusions which may hinder the drilling process and locate shallow aquifers, which will tend to be monitored before and during fracking. Five lines of varying lengths from 2-6 km and different orientation were surveyed using geophysical techniques which included the time domain, electrical resistivity as well as the magnetic methods. The geophysical investigations were conducted in order to fulfil the main vii objectives and aims of the study. The techniques were then complemented by XRF, petrography and density analyses. The time domain method reveals that Lines 1 and Line 2 comprises of rocks with low resistivity values of less than 30 Ωm. These two lines were more conductive towards the east and northeast side of the study site and the resistivity tends to increase towards the south and west. Line 4 appeared to be more conductive towards the south-east of the line. Lines 3 and 5 showed high resistivity values with Line 5 being the most resistive suggesting that the underlying strata is consolidated, dry and hard. The resistivity in all the lines surveyed showed an increase with increasing depth. The depth of investigation was approximately up to 150 m. The electrical resistivity results were only obtained from Line 1 and Line 2. The results, which complement the time domain results, suggest that the two lines indeed comprise of rocks which exhibit low resistivity values. This then implies that the rocks in the vicinity are fractured and the fractures might be field with groundwater and possibly contain some conductive minerals such as sodium oxide (Na2O) as supported by XRF results. This method also reveals that along Line 1, there are individual bodies of high resistivity values. The probing depth of about 78 m was achieved for the electrical resistivity survey. The magnetic data indicate that the magnetic intensity does not vary much across lines 3, 4 and 5 and there is not much change in lithology with depth and lateral extent. The area covered by Lines 1 and 2, and the additional eleven fill in lines has several prominent magnetic highs that are inferred to be due to dolerite intrusions in the sandstones and these bodies may be connected at depth. One of these anomalies which trends east-west direction partially coincides with a mapped dolerite intrusion. Some areas with sandstones also exhibit high viii magnetism which is due to about 3.3% content of Fe2O3 present in the sandstones as seen from XRF. This is probably resulted from weathering of magnetite in dolerite intrusions. Petrography results obtained from samples collected in the field reveal that there is an abundance of quartz in sandstones and siltstones. Quartz arenite also contained about 50% of feldspar, containing both plagioclase and K-feldspar. The sandstones of the Teekloof Formation (Beaufort West) have undergone calcite replacement where the calcite matrix replaced most or all of the clay matrix between the mineral grains. The fractures in rocks were seen and it was inferred that they are due to the present cleavages in feldspar and weathering of feldspar in sandstones. Through X-ray Fluorescence (XRF) analysis, it was revealed that the rocks contained a high percentage of Na2O. XRF also showed that these rocks were under low chemical weathering which led to the formation of new minerals in rock as other minerals such as feldspar weather away. This resulted in quartz being the most dominant mineral present as it is not prone to chemical weathering and thus the rocks were proven to be chemically matured. Thirty one rock samples were collected from the field in order to determine the density and porosity measurements. The fine to medium grained grey sandstones show an average density of 2.597 g/cm3 and a porosity of 1.4 %. The brownish grey sandstone have an average density of 2.546 g/cm3 and porosity of 2.9 % and the brown sandstones exhibit an average of 2.584 g/cm3 and a porosity 1.46 %. Dolerite has an average density of 2.970 g/cm3 and porosity of 0.5 % and siltstones have a density of 2.595 g/cm3 and porosity of 1.38 %. The brownish grey sandstones have the highest porosity and the grey sandstone and siltstone have the least porosity after the dolerite.
- Full Text:
- Date Issued: 2018
- Authors: Ntunja, Asanda
- Date: 2018
- Subjects: Geology Geophysics
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/14659 , vital:40034
- Description: Beaufort West lies in the Central Karoo Basin and is specifically situated in the Poortjie Member of the Teekloof Formation in the Beaufort Group. The Teekloof Formation was deposited in a floodplain environment and it consists of alternating bluish grey mudstone and very fine to medium grained sandstone. The sediments of the Karoo Basin were intruded by numerous dolerite sills and dykes during a period of extensive magmatic activity over the whole South African subcontinent when the break-up of Gondwanda occurred. This led to a continuous jointing of the sedimentary rocks and the jointing resulted in rocks that are prone to weathering. Weathering that occurred in Beaufort West resulted in the formation of alluvium, calcrete, scree and hard pan deposits which overlay most of the ground surface. The joints in rocks paved way for groundwater storage in the sedimentary beds as sediments of the Karoo Basin lack significant primary porosity. Geophysical surveys were done in order to determine a suitable site to locate and drill a deep borehole to the White Hill Formation in order to extract shale gas that is proposed to be present in the area, thereafter determine sites for monitoring boreholes for groundwater and also look for area where potential groundwater might be present. This then requires geophysical investigations of the surface and subsurface geology of the area prior to drilling so as to locate any features such as dolerite intrusions which may hinder the drilling process and locate shallow aquifers, which will tend to be monitored before and during fracking. Five lines of varying lengths from 2-6 km and different orientation were surveyed using geophysical techniques which included the time domain, electrical resistivity as well as the magnetic methods. The geophysical investigations were conducted in order to fulfil the main vii objectives and aims of the study. The techniques were then complemented by XRF, petrography and density analyses. The time domain method reveals that Lines 1 and Line 2 comprises of rocks with low resistivity values of less than 30 Ωm. These two lines were more conductive towards the east and northeast side of the study site and the resistivity tends to increase towards the south and west. Line 4 appeared to be more conductive towards the south-east of the line. Lines 3 and 5 showed high resistivity values with Line 5 being the most resistive suggesting that the underlying strata is consolidated, dry and hard. The resistivity in all the lines surveyed showed an increase with increasing depth. The depth of investigation was approximately up to 150 m. The electrical resistivity results were only obtained from Line 1 and Line 2. The results, which complement the time domain results, suggest that the two lines indeed comprise of rocks which exhibit low resistivity values. This then implies that the rocks in the vicinity are fractured and the fractures might be field with groundwater and possibly contain some conductive minerals such as sodium oxide (Na2O) as supported by XRF results. This method also reveals that along Line 1, there are individual bodies of high resistivity values. The probing depth of about 78 m was achieved for the electrical resistivity survey. The magnetic data indicate that the magnetic intensity does not vary much across lines 3, 4 and 5 and there is not much change in lithology with depth and lateral extent. The area covered by Lines 1 and 2, and the additional eleven fill in lines has several prominent magnetic highs that are inferred to be due to dolerite intrusions in the sandstones and these bodies may be connected at depth. One of these anomalies which trends east-west direction partially coincides with a mapped dolerite intrusion. Some areas with sandstones also exhibit high viii magnetism which is due to about 3.3% content of Fe2O3 present in the sandstones as seen from XRF. This is probably resulted from weathering of magnetite in dolerite intrusions. Petrography results obtained from samples collected in the field reveal that there is an abundance of quartz in sandstones and siltstones. Quartz arenite also contained about 50% of feldspar, containing both plagioclase and K-feldspar. The sandstones of the Teekloof Formation (Beaufort West) have undergone calcite replacement where the calcite matrix replaced most or all of the clay matrix between the mineral grains. The fractures in rocks were seen and it was inferred that they are due to the present cleavages in feldspar and weathering of feldspar in sandstones. Through X-ray Fluorescence (XRF) analysis, it was revealed that the rocks contained a high percentage of Na2O. XRF also showed that these rocks were under low chemical weathering which led to the formation of new minerals in rock as other minerals such as feldspar weather away. This resulted in quartz being the most dominant mineral present as it is not prone to chemical weathering and thus the rocks were proven to be chemically matured. Thirty one rock samples were collected from the field in order to determine the density and porosity measurements. The fine to medium grained grey sandstones show an average density of 2.597 g/cm3 and a porosity of 1.4 %. The brownish grey sandstone have an average density of 2.546 g/cm3 and porosity of 2.9 % and the brown sandstones exhibit an average of 2.584 g/cm3 and a porosity 1.46 %. Dolerite has an average density of 2.970 g/cm3 and porosity of 0.5 % and siltstones have a density of 2.595 g/cm3 and porosity of 1.38 %. The brownish grey sandstones have the highest porosity and the grey sandstone and siltstone have the least porosity after the dolerite.
- Full Text:
- Date Issued: 2018
Isolation and characterization of E. coli and Campylobacter spp. from diarrhoeal samples collected from selected hospitals in Amathole District Municipality, Eastern Cape, South Africa
- Authors: Omolajaiye, Sunday Abraham
- Date: 2018
- Subjects: Escherichia coli infections Campylobacter infections Diarrhea
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/6213 , vital:29515
- Description: Approximately 2-4 billion cases of infectious diarrhoea occur every year, with the highest numbers recorded in sub-Saharan Africa. It remains the most common public health issue among children in developing nations. The purpose of this research was to unfold the prevalence of diarrhoeagenic E. coli and Campylobacter pathotypes as well as elucidate their antibiogram characteristics in diarrhoeal stool samples collected in some medical facilities in Eastern Cape Province, South Africa. Two hundred stool samples were collected from both inpatients and outpatients from male and females of all age groups attending selected medical facilities in the study area. Isolation and characterization of both organisms were done using culture based and molecular methods. Antibiotic susceptibility patterns of identified isolates were determined against a panel of 12 antimicrobial agents. One hundred and twenty presumptive E. coli isolates and 42 presumptive isolates of Campylobacter spp. Were isolated. Eighty-two percent (82 percent) of the presumptive E. coli isolates were confirmed as E. coli while 46.3 percent belonged to Campylobacter spp. Pathotyping of the diarrhoeagenic E. coli isolates by Polymerase chain reaction (PCR) showed the following prevalences: DAEC 43 (32 percent), EHEC 18 (17 percent), EIEC 11 (10 percent) and EPEC 18 (17 percent). EAEC and ETEC were not detected, while for Campylobacter spp. 37 (88 percent) were C. jejuni, and C. coli was not detected. A total of 12 (32.4 percent) of the confirmed Campylobacter jejuni isolates were found to possess the fliM gene, 9 (24.3 percent) possessed the flhA gene and only 6 (16.2 percent) harboured the gene flgE2. None were positive for the flaA, flab and flhB genes.The antibiotic resistance patterns observed among the E. coli isolates were high against ampicillin (98.1 percent), chloramphenicol (94.3 percent) and tetracycline (90.6 percent). For Campylobacter spp., resistance observed were: chloramphenicol (91.6 percent), tetracycline (25.2 percent), erythromycin (49.6 percent) and gentamycin (56.4 percent). A lesser resistance against imipenem (35.9 percent) and quinolone (ciprofloxacin) (45.5 percent) were exhibited by the E.coli isolates. 10.8 percent and 20.3 percent of the Campylobacter isolates were resistant to imipenem and ciprofloxacin respectively. The presence of chloramphenicol (CatA1) and tetracycline (tetA) resistance genes were detected in 94 percent and 89 percent of E. coli isolates respectively while 98 percent of Campylobacter spp. Harboured the catA1 resistance gene. It could be deduced from this study that E. coli and Campylobacter spp. are predomiant enteric pathogens as the etiologic agents of diarrhoea in the study community, and that their antimicrobial resistance is high in the study location. The need to develop strategies to prevent infection and control resistant organisms is evident.
- Full Text:
- Date Issued: 2018
- Authors: Omolajaiye, Sunday Abraham
- Date: 2018
- Subjects: Escherichia coli infections Campylobacter infections Diarrhea
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/6213 , vital:29515
- Description: Approximately 2-4 billion cases of infectious diarrhoea occur every year, with the highest numbers recorded in sub-Saharan Africa. It remains the most common public health issue among children in developing nations. The purpose of this research was to unfold the prevalence of diarrhoeagenic E. coli and Campylobacter pathotypes as well as elucidate their antibiogram characteristics in diarrhoeal stool samples collected in some medical facilities in Eastern Cape Province, South Africa. Two hundred stool samples were collected from both inpatients and outpatients from male and females of all age groups attending selected medical facilities in the study area. Isolation and characterization of both organisms were done using culture based and molecular methods. Antibiotic susceptibility patterns of identified isolates were determined against a panel of 12 antimicrobial agents. One hundred and twenty presumptive E. coli isolates and 42 presumptive isolates of Campylobacter spp. Were isolated. Eighty-two percent (82 percent) of the presumptive E. coli isolates were confirmed as E. coli while 46.3 percent belonged to Campylobacter spp. Pathotyping of the diarrhoeagenic E. coli isolates by Polymerase chain reaction (PCR) showed the following prevalences: DAEC 43 (32 percent), EHEC 18 (17 percent), EIEC 11 (10 percent) and EPEC 18 (17 percent). EAEC and ETEC were not detected, while for Campylobacter spp. 37 (88 percent) were C. jejuni, and C. coli was not detected. A total of 12 (32.4 percent) of the confirmed Campylobacter jejuni isolates were found to possess the fliM gene, 9 (24.3 percent) possessed the flhA gene and only 6 (16.2 percent) harboured the gene flgE2. None were positive for the flaA, flab and flhB genes.The antibiotic resistance patterns observed among the E. coli isolates were high against ampicillin (98.1 percent), chloramphenicol (94.3 percent) and tetracycline (90.6 percent). For Campylobacter spp., resistance observed were: chloramphenicol (91.6 percent), tetracycline (25.2 percent), erythromycin (49.6 percent) and gentamycin (56.4 percent). A lesser resistance against imipenem (35.9 percent) and quinolone (ciprofloxacin) (45.5 percent) were exhibited by the E.coli isolates. 10.8 percent and 20.3 percent of the Campylobacter isolates were resistant to imipenem and ciprofloxacin respectively. The presence of chloramphenicol (CatA1) and tetracycline (tetA) resistance genes were detected in 94 percent and 89 percent of E. coli isolates respectively while 98 percent of Campylobacter spp. Harboured the catA1 resistance gene. It could be deduced from this study that E. coli and Campylobacter spp. are predomiant enteric pathogens as the etiologic agents of diarrhoea in the study community, and that their antimicrobial resistance is high in the study location. The need to develop strategies to prevent infection and control resistant organisms is evident.
- Full Text:
- Date Issued: 2018
Knowledge, attitudes and practice of professional nurses regarding organ donation in critical care units of public and private hospitals in Eastern Cape Province
- Authors: Green, Bukelwa
- Date: 2018
- Subjects: Organ donors--Supply and demand Donation of organs, tissues, etc Transplantation of organs, tissues
- Language: English
- Type: Thesis , Masters , Magister Curationis
- Identifier: http://hdl.handle.net/10353/11116 , vital:37138
- Description: Globally there is an overwhelming increase in the number of patients waiting for donated organs for transplantation, with a gross shortage of available organs. There are many contributing factors to this dearth of donated organs, including level of knowledge, cultural beliefs, lack of clear practice and the attitudes of health care providers, as the gatekeepers of the health system. This non-experimental research study assessed the attitudes, level of knowledge, and practices of professional nurses working in critical care units in public and private hospitals in Eastern Cape Province regarding organ donation. The study was conducted in both private and public hospital intensive care and renal care units. The targeted population in the study was professional nurses working in intensive and renal care units, trained in critical care or nephrology, as well as those who were not trained but working in these specialised areas. The non-probability, purposive sampling method was employed and total population was 187 professional nurses working in public and private critical care units but only 108 managed to yield data for this study. Data was collected with anonymous, self - developed questionnaires. The questionnaire was submitted to supervisors and statistician for validity. Reliability was established by conducting pretest of the instrument, which yielded positive results because all questionnaires were correctly completed.Data captured and analysed using the Statistical Package for Social Sciences (SPSS) software for Windows version 22 and is presented by means of percentages and tables. Results: The findings were that, there is insufficient knowledge on the topic of organ donation, with subsequent inadequate practice, which derives from lack of pre-registration training and ongoing in-service education regarding the topic. The unavailability of full organ transplantation services in the province is viewed as a hindrance in the practice of organ donation. There was generally a positive attitude towards organ donation, particularly among Christian nurses. The knowledge deficit and limited practice seem not to have affected the attitudes of the participants, who supported the idea of organ donation. However, despite the positive attitudes, only 13 participants (12.0 percent) agreed to be registered as organ donors. 50 percent of participants lacked knowledge about availability and contact details of the provincial organ donation foundation, which may indicate inadequate marketing on the part of the foundation. There was no significant difference in knowledge, attitudes and practice between nurses in the private and public sectors. Rather differences were noted between the different levels of health care services, i.e. between the secondary and tertiary level. The tertiary level outperformed the secondary level institutions. It is recommended that organ donation be incorporated into the syllabi of health sciences courses both in universities and colleges, and that awareness campaigns be strengthened.
- Full Text:
- Date Issued: 2018
- Authors: Green, Bukelwa
- Date: 2018
- Subjects: Organ donors--Supply and demand Donation of organs, tissues, etc Transplantation of organs, tissues
- Language: English
- Type: Thesis , Masters , Magister Curationis
- Identifier: http://hdl.handle.net/10353/11116 , vital:37138
- Description: Globally there is an overwhelming increase in the number of patients waiting for donated organs for transplantation, with a gross shortage of available organs. There are many contributing factors to this dearth of donated organs, including level of knowledge, cultural beliefs, lack of clear practice and the attitudes of health care providers, as the gatekeepers of the health system. This non-experimental research study assessed the attitudes, level of knowledge, and practices of professional nurses working in critical care units in public and private hospitals in Eastern Cape Province regarding organ donation. The study was conducted in both private and public hospital intensive care and renal care units. The targeted population in the study was professional nurses working in intensive and renal care units, trained in critical care or nephrology, as well as those who were not trained but working in these specialised areas. The non-probability, purposive sampling method was employed and total population was 187 professional nurses working in public and private critical care units but only 108 managed to yield data for this study. Data was collected with anonymous, self - developed questionnaires. The questionnaire was submitted to supervisors and statistician for validity. Reliability was established by conducting pretest of the instrument, which yielded positive results because all questionnaires were correctly completed.Data captured and analysed using the Statistical Package for Social Sciences (SPSS) software for Windows version 22 and is presented by means of percentages and tables. Results: The findings were that, there is insufficient knowledge on the topic of organ donation, with subsequent inadequate practice, which derives from lack of pre-registration training and ongoing in-service education regarding the topic. The unavailability of full organ transplantation services in the province is viewed as a hindrance in the practice of organ donation. There was generally a positive attitude towards organ donation, particularly among Christian nurses. The knowledge deficit and limited practice seem not to have affected the attitudes of the participants, who supported the idea of organ donation. However, despite the positive attitudes, only 13 participants (12.0 percent) agreed to be registered as organ donors. 50 percent of participants lacked knowledge about availability and contact details of the provincial organ donation foundation, which may indicate inadequate marketing on the part of the foundation. There was no significant difference in knowledge, attitudes and practice between nurses in the private and public sectors. Rather differences were noted between the different levels of health care services, i.e. between the secondary and tertiary level. The tertiary level outperformed the secondary level institutions. It is recommended that organ donation be incorporated into the syllabi of health sciences courses both in universities and colleges, and that awareness campaigns be strengthened.
- Full Text:
- Date Issued: 2018
Laccase production by selected bacteria species isolated from some aquatic and terrestrial milieu of the Eastern Cape : applications in wastewater treatment
- Authors: Unuofin, John Onolame
- Date: 2018
- Subjects: Laccase Sewage -- Purification -- Biological treatment Water -- Purification
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10353/9626 , vital:34810
- Description: Aromatic pollutants are a diverse group of chemicals which are continuously produced from industrialization, urbanization and sophistication in technological advancement. Pristine water source polluted by these chemicls makes the water unsafe for human consumption, and as well disrupts the trophic structure of the aquatic milieu. Physico-chemical treatment techniques employed so far have been accompanied by major drawbacks which have overriden the relative successes recorded, hence, greener, simpler and more efficient methods of pollutant transformation is imperative. The prospect of enzymatic treatment of pollutants has gradually been receiving growing attention in contemporary times due to the their environmental friendliness and production economic feasiblity. Laccase, a multicopper oxidase has heightened its appeal towards environmental and biotechnological applications due to its broad substrate specificity and its requirement of atmospheric molecular oxygen as a cosubstrate and the discharge of water as the byproduct. Hence, this present study was designed to evaluate the biotechnological potentials of laccases produced by some bacteria species from some aquatic biomes of the Eastern Cape Province, South Africa. The laccase-producing bacteria were isolated from selected environmental samples by selective enrichment using selective aromatic compounds as sole carbon source and subsequently, laccase-screening phenolic substrates. The laccase-producing bacteria were identified by molecular techniques as proteobacteria belonging to the following genera: Achromobacter, Bordetella, Citrobacter, Pseudomonas and Stenotrophomonas. Optimisation of laccase production in a submerged fermentation was by traditional and statistical methods, where four isolates (Hb9c; Achromobacter xylosoxidans HWN16, Hb16c; Bordetella bronchisepta HSO16, Berl11b2; Stenotrophomonas maltophilia BIJ16, Ie1c; Citrobacter freundii LLJ16) were evaluated for the fermentative production of laccase from lignocellulosic agroindustrial residues. Predictions from statistical optimisation showed that weakly acidic conditions (pH 5) and low agitation speed (100 rpm) were required for maximum laccase production from mandarin peelings (0.5 g/200 mL) and NaNO3 (0.25 g/200 mL) in Hb9c, maize stover (0.50 g/200 mL) and NaNO3 (0.050 g/200mL) in Berl11b2 while a lower agitation speed (50 rpm) was required for maximum laccase output from 2.0 g/200 mL maize stover and 0.050 g/200 mL KNO3 in Ie1c. However, 2.50 g/200 mL wheat bran, 0.050 g/200 mL yeast extract and 50 rpm agitiation under acidic conditions (pH3) yielded maximum laccase titres in Hb16c. Further characterisation of Hb9c and Ie1c laccase secretions portrayed their polyextremotolerant capacities. They were active at a broad range of tempertaure (0-90 degreesC); with optima at 70°C (Hb9c) and 60°C (Ie1c), pH (3-11); with optima at pH 6 (Ie1c) and pH 8 (Hb9c), respectively, and were equally thermo- and pH-stable. Their activities were either improved or left unabated by high concentrations of cations, detergents, and chloride. In addition, catalytic activities of Hb9c and Ie1c laccase secretions increased when they were preincubated with 2 – 20 percent of fluoride, a potent inhibitor. Consequently, a molecular perspective depicted the isolates to have multiple homologous laccase encoding genes. The enzymes were successfully immobilised on solid supports comprising gelatin and Na-alginate with a recovery of cca. 85 percent residual activity after 8 cycles of oprertional stability experiments. The immobilised laccases were remarkable in the decolourisation of synthetic dyes, albeit, free forms also elicited satisfactory performances. Ultimately, the application of free laccases in denim bleaching, individually or with a blend of a mediator, ABTS, showed that denim colours could be bleached without the need for chemical bleaching agents. The results obtained suggest the bacteria laccases produced from lignocellulosic wastes may serve as potent degraders of phenolic pollutants in water and, may also contribute to the bioeconomy and promote greener techniques for industrial applications.
- Full Text:
- Date Issued: 2018
- Authors: Unuofin, John Onolame
- Date: 2018
- Subjects: Laccase Sewage -- Purification -- Biological treatment Water -- Purification
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10353/9626 , vital:34810
- Description: Aromatic pollutants are a diverse group of chemicals which are continuously produced from industrialization, urbanization and sophistication in technological advancement. Pristine water source polluted by these chemicls makes the water unsafe for human consumption, and as well disrupts the trophic structure of the aquatic milieu. Physico-chemical treatment techniques employed so far have been accompanied by major drawbacks which have overriden the relative successes recorded, hence, greener, simpler and more efficient methods of pollutant transformation is imperative. The prospect of enzymatic treatment of pollutants has gradually been receiving growing attention in contemporary times due to the their environmental friendliness and production economic feasiblity. Laccase, a multicopper oxidase has heightened its appeal towards environmental and biotechnological applications due to its broad substrate specificity and its requirement of atmospheric molecular oxygen as a cosubstrate and the discharge of water as the byproduct. Hence, this present study was designed to evaluate the biotechnological potentials of laccases produced by some bacteria species from some aquatic biomes of the Eastern Cape Province, South Africa. The laccase-producing bacteria were isolated from selected environmental samples by selective enrichment using selective aromatic compounds as sole carbon source and subsequently, laccase-screening phenolic substrates. The laccase-producing bacteria were identified by molecular techniques as proteobacteria belonging to the following genera: Achromobacter, Bordetella, Citrobacter, Pseudomonas and Stenotrophomonas. Optimisation of laccase production in a submerged fermentation was by traditional and statistical methods, where four isolates (Hb9c; Achromobacter xylosoxidans HWN16, Hb16c; Bordetella bronchisepta HSO16, Berl11b2; Stenotrophomonas maltophilia BIJ16, Ie1c; Citrobacter freundii LLJ16) were evaluated for the fermentative production of laccase from lignocellulosic agroindustrial residues. Predictions from statistical optimisation showed that weakly acidic conditions (pH 5) and low agitation speed (100 rpm) were required for maximum laccase production from mandarin peelings (0.5 g/200 mL) and NaNO3 (0.25 g/200 mL) in Hb9c, maize stover (0.50 g/200 mL) and NaNO3 (0.050 g/200mL) in Berl11b2 while a lower agitation speed (50 rpm) was required for maximum laccase output from 2.0 g/200 mL maize stover and 0.050 g/200 mL KNO3 in Ie1c. However, 2.50 g/200 mL wheat bran, 0.050 g/200 mL yeast extract and 50 rpm agitiation under acidic conditions (pH3) yielded maximum laccase titres in Hb16c. Further characterisation of Hb9c and Ie1c laccase secretions portrayed their polyextremotolerant capacities. They were active at a broad range of tempertaure (0-90 degreesC); with optima at 70°C (Hb9c) and 60°C (Ie1c), pH (3-11); with optima at pH 6 (Ie1c) and pH 8 (Hb9c), respectively, and were equally thermo- and pH-stable. Their activities were either improved or left unabated by high concentrations of cations, detergents, and chloride. In addition, catalytic activities of Hb9c and Ie1c laccase secretions increased when they were preincubated with 2 – 20 percent of fluoride, a potent inhibitor. Consequently, a molecular perspective depicted the isolates to have multiple homologous laccase encoding genes. The enzymes were successfully immobilised on solid supports comprising gelatin and Na-alginate with a recovery of cca. 85 percent residual activity after 8 cycles of oprertional stability experiments. The immobilised laccases were remarkable in the decolourisation of synthetic dyes, albeit, free forms also elicited satisfactory performances. Ultimately, the application of free laccases in denim bleaching, individually or with a blend of a mediator, ABTS, showed that denim colours could be bleached without the need for chemical bleaching agents. The results obtained suggest the bacteria laccases produced from lignocellulosic wastes may serve as potent degraders of phenolic pollutants in water and, may also contribute to the bioeconomy and promote greener techniques for industrial applications.
- Full Text:
- Date Issued: 2018