Expanding access to essential medicines through the right to health: a case study of South Africa
- Authors: Motamakore, Shelton Tapiwa
- Date: 2015
- Subjects: Right to health -- South Africa Medical care -- Law and legislation -- South Africa AIDS (Disease) -- Government policy -- South Africa
- Language: English
- Type: Thesis , Masters , LLM
- Identifier: http://hdl.handle.net/10353/5620 , vital:29352
- Description: Lack of access to essential medicines has proven to be a persisting problem which is in conflict with the goal of realising the right to health envisaged by the South African constitution and international human rights instruments. With more than twenty years of democracy, South Africa is still plaguing with a multiplicity of pandemics such as HIV and AIDS, cancer, malaria, tuberculosis, among others, leading to premature death and untold suffering of the people. According to a 2015 United Nations AIDS (UNAIDS) Gap report, South Africa is still regarded as the epicentre of HIV and other infectious diseases. The 2015 UNAIDS Gap report states that South Africa has more women than men living with HIV and AIDS. The report further indicates that the impact of this pandemic is worsened by the inaccessibility of essential medicines that are vital for life saving. This dissertation posits that the epidemiological health crisis described above can be largely eradicated through the utilisation of the right to health. The right to health, according to this dissertation, contains a legal and transformative power which can be utilised to limit the negative impact of patent laws on access to essential medicines in South Africa. This dissertation validates the long held view that World Trade Organisation (WTO) intellectual property laws have contributed to the inaccessibility of essential medicines through causing patent ever greening, patent linkages and pharmaceutical company’s monopolies. Consequently, many marginalised groups in South Africa lack access to essential medicines owing to the higher prices charged for such medicines thus violating the right to health, life and other fundamental human rights. The right to health which is the immediate right infringed when there is lack of access to essential medicines form the core theme of this dissertation. This dissertation argues that access to essential medicine is a fundamental part of the right to health protected under international and national human rights instruments. This dissertation further argue that the right to health imposes obligations which requires South African government to take reasonable legislative and other measures, within its available resources, to provide access to essential medicines. The dissertation‘s key contribution is its proposed solutions on how to ensure that patents rules in South Africa are tamed with obligations consistent with the right to health. If properly implemented, these solutions have the potential to give greater specification to the normative commitments imposed by the right to health in the patent claims scenarios.
- Full Text:
- Date Issued: 2015
- Authors: Motamakore, Shelton Tapiwa
- Date: 2015
- Subjects: Right to health -- South Africa Medical care -- Law and legislation -- South Africa AIDS (Disease) -- Government policy -- South Africa
- Language: English
- Type: Thesis , Masters , LLM
- Identifier: http://hdl.handle.net/10353/5620 , vital:29352
- Description: Lack of access to essential medicines has proven to be a persisting problem which is in conflict with the goal of realising the right to health envisaged by the South African constitution and international human rights instruments. With more than twenty years of democracy, South Africa is still plaguing with a multiplicity of pandemics such as HIV and AIDS, cancer, malaria, tuberculosis, among others, leading to premature death and untold suffering of the people. According to a 2015 United Nations AIDS (UNAIDS) Gap report, South Africa is still regarded as the epicentre of HIV and other infectious diseases. The 2015 UNAIDS Gap report states that South Africa has more women than men living with HIV and AIDS. The report further indicates that the impact of this pandemic is worsened by the inaccessibility of essential medicines that are vital for life saving. This dissertation posits that the epidemiological health crisis described above can be largely eradicated through the utilisation of the right to health. The right to health, according to this dissertation, contains a legal and transformative power which can be utilised to limit the negative impact of patent laws on access to essential medicines in South Africa. This dissertation validates the long held view that World Trade Organisation (WTO) intellectual property laws have contributed to the inaccessibility of essential medicines through causing patent ever greening, patent linkages and pharmaceutical company’s monopolies. Consequently, many marginalised groups in South Africa lack access to essential medicines owing to the higher prices charged for such medicines thus violating the right to health, life and other fundamental human rights. The right to health which is the immediate right infringed when there is lack of access to essential medicines form the core theme of this dissertation. This dissertation argues that access to essential medicine is a fundamental part of the right to health protected under international and national human rights instruments. This dissertation further argue that the right to health imposes obligations which requires South African government to take reasonable legislative and other measures, within its available resources, to provide access to essential medicines. The dissertation‘s key contribution is its proposed solutions on how to ensure that patents rules in South Africa are tamed with obligations consistent with the right to health. If properly implemented, these solutions have the potential to give greater specification to the normative commitments imposed by the right to health in the patent claims scenarios.
- Full Text:
- Date Issued: 2015
Experiences of HIV positive clients defaulting isoniazid preventive therapy (IPT) in King Williams Town area under the Buffalo City Municipality in the Eastern Cape Province
- Authors: Williams, Nelisa Colleen
- Date: 2015
- Subjects: AIDS (Disease) -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc (Community Nursing Science)
- Identifier: vital:11923 , http://hdl.handle.net/10353/d1020177 , AIDS (Disease) -- South Africa -- Eastern Cape
- Description: This qualitative study using a phenomenological design, aimed at exploring and describing and exploring the experiences of HIV positive clients defaulting Isoniazid Preventive Therapy services in the Bhisho Primary Health Care Services. The emphasis was on the factors leading clients to default. An in- depth unstructured face to face interviews were done on 14 participants from 4 clinics or facilities under Bhisho Primary Health Care Services. From the respondents’ responses it can be noted that work and family related issues, ignorance of patients, side effects, and negligence of nurses and denial of HIV status were identified as reasons for defaulting. Having knowledge about the treatment and health providers’ attitudes to patients also played a role in patients defaulting their treatment.Many suggestions were then put forward by the respondents to curb defaulting among patients. These included the use of text message reminders, not to discrimination HIV patients because of their status, to use consulting rooms for privacy and also nurses and caregivers to control their attitudes when dealing with patients.
- Full Text:
- Date Issued: 2015
- Authors: Williams, Nelisa Colleen
- Date: 2015
- Subjects: AIDS (Disease) -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc (Community Nursing Science)
- Identifier: vital:11923 , http://hdl.handle.net/10353/d1020177 , AIDS (Disease) -- South Africa -- Eastern Cape
- Description: This qualitative study using a phenomenological design, aimed at exploring and describing and exploring the experiences of HIV positive clients defaulting Isoniazid Preventive Therapy services in the Bhisho Primary Health Care Services. The emphasis was on the factors leading clients to default. An in- depth unstructured face to face interviews were done on 14 participants from 4 clinics or facilities under Bhisho Primary Health Care Services. From the respondents’ responses it can be noted that work and family related issues, ignorance of patients, side effects, and negligence of nurses and denial of HIV status were identified as reasons for defaulting. Having knowledge about the treatment and health providers’ attitudes to patients also played a role in patients defaulting their treatment.Many suggestions were then put forward by the respondents to curb defaulting among patients. These included the use of text message reminders, not to discrimination HIV patients because of their status, to use consulting rooms for privacy and also nurses and caregivers to control their attitudes when dealing with patients.
- Full Text:
- Date Issued: 2015
Experiences of incarcerated women who have committed intimate partner homicide
- Authors: Coombs-Brown, Chanelle
- Date: 2015
- Subjects: Incarcerated women Female offenders Women--Violence against
- Language: English
- Type: Thesis , Masters , Psychology
- Identifier: http://hdl.handle.net/10353/17406 , vital:40965
- Description: This qualitative study explores the experiences of incarcerated women who have perpetrated intimate partner homicide (IPH) and had been exposed to intimate partner violence (IPV) in their relationships. The study further explores women’s experiences of IPV, in particular those which motivated them to perpetrate IPH. The study examines how factors associated with IPV affect women to the point of responding with such lethal IPH. Additionally, it explores whether or not women feel remorse for their actions of IPH. The current study falls within an interpretive research paradigm and employs interpretative phenomenological analysis (IPA) as its theoretical framework. The researcher selected IPA as it works well in understanding and describing participants’ experiences. Semi-structured face to face interviews were conducted with nine women incarcerated for IPH. The findings of the study were analysed using the IPA approach. Three main themes emerged from the analysis: mainly all women reported to have experienced IPV in their respective relationships with the deceased, all, except one woman, reported that their partners had committed infidelity, the women also reported to have experienced much disappointment in gaining assistance from the judicial system. Therefore, on-going IPV including death threats resulted in fear; infidelity resulted in tremendous emotional and psychological strain, as well as feelings of betrayal; and perceived lack of support from the judicial system all appeared to have motivated women to perpetrate IPH with the majority indicating remorse.
- Full Text:
- Date Issued: 2015
- Authors: Coombs-Brown, Chanelle
- Date: 2015
- Subjects: Incarcerated women Female offenders Women--Violence against
- Language: English
- Type: Thesis , Masters , Psychology
- Identifier: http://hdl.handle.net/10353/17406 , vital:40965
- Description: This qualitative study explores the experiences of incarcerated women who have perpetrated intimate partner homicide (IPH) and had been exposed to intimate partner violence (IPV) in their relationships. The study further explores women’s experiences of IPV, in particular those which motivated them to perpetrate IPH. The study examines how factors associated with IPV affect women to the point of responding with such lethal IPH. Additionally, it explores whether or not women feel remorse for their actions of IPH. The current study falls within an interpretive research paradigm and employs interpretative phenomenological analysis (IPA) as its theoretical framework. The researcher selected IPA as it works well in understanding and describing participants’ experiences. Semi-structured face to face interviews were conducted with nine women incarcerated for IPH. The findings of the study were analysed using the IPA approach. Three main themes emerged from the analysis: mainly all women reported to have experienced IPV in their respective relationships with the deceased, all, except one woman, reported that their partners had committed infidelity, the women also reported to have experienced much disappointment in gaining assistance from the judicial system. Therefore, on-going IPV including death threats resulted in fear; infidelity resulted in tremendous emotional and psychological strain, as well as feelings of betrayal; and perceived lack of support from the judicial system all appeared to have motivated women to perpetrate IPH with the majority indicating remorse.
- Full Text:
- Date Issued: 2015
Exploring the effects of domestic violence in the psychosocial development of children : the case of Mlungisi Township in Queenstown
- Authors: Ntsumpa, SL
- Date: 2015
- Subjects: Family violence Abused children--South Africa Children and violence
- Language: English
- Type: Thesis , Masters , Social Work
- Identifier: http://hdl.handle.net/10353/17338 , vital:40957
- Description: The main aim of the study was to explore the effects of domestic violence in the psychosocial development of children at Mlungisi Township, Chris Hani District in Queenstown – Eastern Cape Province. The specific objectives of the study were: to investigate the effect domestic violence has on the psychosocial development of children; to assess the behavior of the children subjected to domestic violence; and to examine challenges that children are facing due to domestic violence; and to examine the support provided by professionals (Social works, community worker, educator, South African Police and Justice System) to children who are victims of domestic violence. The study adopted a qualitative research paradigm and non-probability sampling methodology in the form of purposive sampling technique. The study‟s sample size comprised of 20 participants, 7 male children between the ages of 9 and 16, and 8 female children between the ages of 9 and 16 on in-depth one-on-one interviews. Five children withdrew from the study because they became emotional and were reluctant to participate. The study also comprised of 5 participants in a focus group which composed of various professionals from government departments such as: Department of Social Development, Department of Education, South African Police Services, Lukhanji Municipality and Justice Official who are directly working with children. The research instrument used was an interview guide with open ended questions. The study findings include the following: effects of domestic violence on the psychosocial development of children; challenges faced by children due to domestic violence; behaviors of children subjected to domestic violence; support services; efficiency and effectiveness of services rendered to the children; and improvement of services rendered to the victims by government officials. The study recommendations were: more studies on domestic violence specifically on children as victims should be done; policy regarding domestic violence needs to be reviewed and in order to accommodate children; there should be policies that focus solemnly to children who happen to be victims of domestic violence not domestic violence in general; and government departments and other relevant stakeholder should be strengthened or even develop new intervention strategies to ensure effectiveness in curbing domestic violence.
- Full Text:
- Date Issued: 2015
- Authors: Ntsumpa, SL
- Date: 2015
- Subjects: Family violence Abused children--South Africa Children and violence
- Language: English
- Type: Thesis , Masters , Social Work
- Identifier: http://hdl.handle.net/10353/17338 , vital:40957
- Description: The main aim of the study was to explore the effects of domestic violence in the psychosocial development of children at Mlungisi Township, Chris Hani District in Queenstown – Eastern Cape Province. The specific objectives of the study were: to investigate the effect domestic violence has on the psychosocial development of children; to assess the behavior of the children subjected to domestic violence; and to examine challenges that children are facing due to domestic violence; and to examine the support provided by professionals (Social works, community worker, educator, South African Police and Justice System) to children who are victims of domestic violence. The study adopted a qualitative research paradigm and non-probability sampling methodology in the form of purposive sampling technique. The study‟s sample size comprised of 20 participants, 7 male children between the ages of 9 and 16, and 8 female children between the ages of 9 and 16 on in-depth one-on-one interviews. Five children withdrew from the study because they became emotional and were reluctant to participate. The study also comprised of 5 participants in a focus group which composed of various professionals from government departments such as: Department of Social Development, Department of Education, South African Police Services, Lukhanji Municipality and Justice Official who are directly working with children. The research instrument used was an interview guide with open ended questions. The study findings include the following: effects of domestic violence on the psychosocial development of children; challenges faced by children due to domestic violence; behaviors of children subjected to domestic violence; support services; efficiency and effectiveness of services rendered to the children; and improvement of services rendered to the victims by government officials. The study recommendations were: more studies on domestic violence specifically on children as victims should be done; policy regarding domestic violence needs to be reviewed and in order to accommodate children; there should be policies that focus solemnly to children who happen to be victims of domestic violence not domestic violence in general; and government departments and other relevant stakeholder should be strengthened or even develop new intervention strategies to ensure effectiveness in curbing domestic violence.
- Full Text:
- Date Issued: 2015
Exploring the effects of the psychological contract on organisational commitment and employee engagement in a restructured organisational environment: the case of selected hospitals in the Eastern Cape province
- Stofile, Phakama Phumla Bernadette
- Authors: Stofile, Phakama Phumla Bernadette
- Date: 2015
- Subjects: Industrial management -- South Africa -- Eastern Cape Psychology, Industrial -- South Africa -- Eastern Cape Organizational behavior -- South Africa -- Eastern Cape Personnel management -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , M Admin
- Identifier: http://hdl.handle.net/10353/1984 , vital:27591
- Description: This study investigated the impact of organisation restructuring on the psychological contract and the effects on employee engagement and organisational commitment. The study focused on employees from selected health care institutions. The sample consisted of 156 employees from the selected health care institutions. Results indicated positive relationships between employee engagement, psychological contract and organisational commitment. The researcher recommended a better understanding of the manner in which individuals interpret various inducements where would clearer prescriptions be provided. Therefore, employers were advised to be aware of employees’ values and attempt to address them as they are important
- Full Text:
- Date Issued: 2015
- Authors: Stofile, Phakama Phumla Bernadette
- Date: 2015
- Subjects: Industrial management -- South Africa -- Eastern Cape Psychology, Industrial -- South Africa -- Eastern Cape Organizational behavior -- South Africa -- Eastern Cape Personnel management -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , M Admin
- Identifier: http://hdl.handle.net/10353/1984 , vital:27591
- Description: This study investigated the impact of organisation restructuring on the psychological contract and the effects on employee engagement and organisational commitment. The study focused on employees from selected health care institutions. The sample consisted of 156 employees from the selected health care institutions. Results indicated positive relationships between employee engagement, psychological contract and organisational commitment. The researcher recommended a better understanding of the manner in which individuals interpret various inducements where would clearer prescriptions be provided. Therefore, employers were advised to be aware of employees’ values and attempt to address them as they are important
- Full Text:
- Date Issued: 2015
Exploring user experience (UX) factors For ICTD services
- Authors: Nyambi, Pride Bongiwe
- Date: 2015
- Language: English
- Type: Thesis , Masters , MSc (Computer Science)
- Identifier: vital:11400 , http://hdl.handle.net/10353/d1020164
- Description: Consistent with global entities such as the United Nations- through the World Summit of the Information Society (WSIS), introduction of Information and Communication Technology (ICT) for human development has seen the introduction of ICT-based services aimed at facilitating socio-economic development of marginalized communities. The use of ICTs has always solicited the concept of Human Computer Interaction (HCI), which involves the methods which humans interact with technology. The types of User Interfaces (UIs) and interaction techniques that people use to interact with ICTs affects the way they perceive technology and eventually, their acceptance of the technology. Current ICT systems still haven‟t adopted the concept of placing the user at the core of the interaction. Users are still required to adapt themselves to the interface‟s characteristics; which limits the number of people who can use the system due to inabilities to adapt to the interface. As a result, the information embedded in these technologies is still inaccessible and useless to Marginalized Rural Area (MRA) users. Such usability challenges can be mitigated against and avoided by matching UI components with the users‟ mental models, language, preferences, needs and other socio-cultural artefacts. In this research, literature in Human-Computer Interaction (HCI) is reviewed with emphasis on the usability and User Experience (UX) during user interaction with ICTs using various modes of interactions. HCI emphasizes the need for systems to take account of user‟s characteristics such as their abilities, needs, socio-cultural experiences, behaviours and interests. In efforts to meet the requirement of UX, the user, system and the context of use, need to be evaluated, taking into consideration that changing one entity modifies the UX. This will be achieved by persona profiling to determine the key characteristics of the user communities, clustered according to the key UX attributes. Subsequently, through detailed usability evaluations, including the use of System Usability Scale (SUS) to determine user satisfaction with various UI components/techniques per identified persona- thus providing and persona mapping for usability of Information and Communication Technology for Development (ICTD) services. The results from this research are reflective of the importance of creating personas for usability testing. Some of the personas do not have a problem with interacting with most of the interfaces but their choice of interface comes from a preference point of view. For some personas, their skills and level of experience with ICTs motivates their choice of interface. The common UI component that users from across the spectrum appreciate is UI consistency which makes interaction easier and more natural. Common obstacles with current User Interfaces (UIs) that inhibit users from MRAs include the hefty use of text in interfaces, unintuitive navigation structures and the use of a foreign language. Differences in UIs from different application developers present an inconsistency which challenges the users from rural areas. These differences include the layout, the text entry methods and the form of output produced. A solution to this has been identified from the usability test as the use of speech-enabled interfaces in a language that can be understood by the target audience. In addition, through literature study it has been found that UX of interfaces can be improved by the use of less textual or text-free interfaces. Based on literature, users from MRAs can benefit from using hand-writing based UIs for text-based entry which mimics pen and paper environment for literate users who have experience with writing. Finally, the use of numbered options can assist illiterate users in tasks that requires users to choose options and for navigation. Therefore, consistency in UIs designed to be used by MRA users can improve usability of these interfaces and thus, improving the overall UX.
- Full Text:
- Date Issued: 2015
- Authors: Nyambi, Pride Bongiwe
- Date: 2015
- Language: English
- Type: Thesis , Masters , MSc (Computer Science)
- Identifier: vital:11400 , http://hdl.handle.net/10353/d1020164
- Description: Consistent with global entities such as the United Nations- through the World Summit of the Information Society (WSIS), introduction of Information and Communication Technology (ICT) for human development has seen the introduction of ICT-based services aimed at facilitating socio-economic development of marginalized communities. The use of ICTs has always solicited the concept of Human Computer Interaction (HCI), which involves the methods which humans interact with technology. The types of User Interfaces (UIs) and interaction techniques that people use to interact with ICTs affects the way they perceive technology and eventually, their acceptance of the technology. Current ICT systems still haven‟t adopted the concept of placing the user at the core of the interaction. Users are still required to adapt themselves to the interface‟s characteristics; which limits the number of people who can use the system due to inabilities to adapt to the interface. As a result, the information embedded in these technologies is still inaccessible and useless to Marginalized Rural Area (MRA) users. Such usability challenges can be mitigated against and avoided by matching UI components with the users‟ mental models, language, preferences, needs and other socio-cultural artefacts. In this research, literature in Human-Computer Interaction (HCI) is reviewed with emphasis on the usability and User Experience (UX) during user interaction with ICTs using various modes of interactions. HCI emphasizes the need for systems to take account of user‟s characteristics such as their abilities, needs, socio-cultural experiences, behaviours and interests. In efforts to meet the requirement of UX, the user, system and the context of use, need to be evaluated, taking into consideration that changing one entity modifies the UX. This will be achieved by persona profiling to determine the key characteristics of the user communities, clustered according to the key UX attributes. Subsequently, through detailed usability evaluations, including the use of System Usability Scale (SUS) to determine user satisfaction with various UI components/techniques per identified persona- thus providing and persona mapping for usability of Information and Communication Technology for Development (ICTD) services. The results from this research are reflective of the importance of creating personas for usability testing. Some of the personas do not have a problem with interacting with most of the interfaces but their choice of interface comes from a preference point of view. For some personas, their skills and level of experience with ICTs motivates their choice of interface. The common UI component that users from across the spectrum appreciate is UI consistency which makes interaction easier and more natural. Common obstacles with current User Interfaces (UIs) that inhibit users from MRAs include the hefty use of text in interfaces, unintuitive navigation structures and the use of a foreign language. Differences in UIs from different application developers present an inconsistency which challenges the users from rural areas. These differences include the layout, the text entry methods and the form of output produced. A solution to this has been identified from the usability test as the use of speech-enabled interfaces in a language that can be understood by the target audience. In addition, through literature study it has been found that UX of interfaces can be improved by the use of less textual or text-free interfaces. Based on literature, users from MRAs can benefit from using hand-writing based UIs for text-based entry which mimics pen and paper environment for literate users who have experience with writing. Finally, the use of numbered options can assist illiterate users in tasks that requires users to choose options and for navigation. Therefore, consistency in UIs designed to be used by MRA users can improve usability of these interfaces and thus, improving the overall UX.
- Full Text:
- Date Issued: 2015
Factors affecting participation rates in farming in the rural areas of South Africa: case of Amathole District Municipality
- Authors: Zamxaka, Xolisa
- Date: 2015
- Subjects: Agricultural systems -- South Africa -- Eastern Cape , Rural development -- South Africa -- Eastern Cape , Sustainable development -- South Africa -- Eastern Cape , Farms, Small -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc Agric (Agricultural Economics)
- Identifier: vital:11233 , http://hdl.handle.net/10353/d1021291 , Agricultural systems -- South Africa -- Eastern Cape , Rural development -- South Africa -- Eastern Cape , Sustainable development -- South Africa -- Eastern Cape , Farms, Small -- South Africa -- Eastern Cape
- Description: South Africa and the rest of developing countries are faced with poverty and poor rural development. Rural participation in agricultural activities is one of the components that can be used to address the poverty challenge facing the people residing in rural areas. The broad objective of this research is to determine factors affecting participation rate in farming in the rural areas of Amathole District Municipality of Eastern Cape. In this study stratified random sampling method was applied in order to choose a sample out of 30 households that were interviewed 13 people belonged to Participants and 17 people belonged to non-Participants. The results from this study show that women participate a lot in farming activities. The multiple regression model was used to test the participation rates of the people in Amathole region specifically Phumlani area. A number of variables were considered in this study to assess the impact of different variables on participation in farming activities. The results showed that about 57% of the respondents are not participating in farming while 43% of the respondents participate. The farming participants that were interviewed all claim that there is a lack in farming support in the area. When there is no support of any kind, rural people would not be motivated to start development projects on their own. Consequently, this lack of farming support in the Phumlani area may have an influence on the number of farming participants. Therefore, the lack of support in the area may serve as a motivation for non-participants not to be influenced to farm. Rural farming needs to be promoted amongst the youth so as to protect and sustain agricultural growth in rural areas. The study has discovered that the youth of Phumlani is not actively involved in farming activities. Government can provide community members with farming resources so as to promote farming in the area. It would be wiser for the government to provide physical farming resources and implements rather than cash grants.
- Full Text:
- Date Issued: 2015
- Authors: Zamxaka, Xolisa
- Date: 2015
- Subjects: Agricultural systems -- South Africa -- Eastern Cape , Rural development -- South Africa -- Eastern Cape , Sustainable development -- South Africa -- Eastern Cape , Farms, Small -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc Agric (Agricultural Economics)
- Identifier: vital:11233 , http://hdl.handle.net/10353/d1021291 , Agricultural systems -- South Africa -- Eastern Cape , Rural development -- South Africa -- Eastern Cape , Sustainable development -- South Africa -- Eastern Cape , Farms, Small -- South Africa -- Eastern Cape
- Description: South Africa and the rest of developing countries are faced with poverty and poor rural development. Rural participation in agricultural activities is one of the components that can be used to address the poverty challenge facing the people residing in rural areas. The broad objective of this research is to determine factors affecting participation rate in farming in the rural areas of Amathole District Municipality of Eastern Cape. In this study stratified random sampling method was applied in order to choose a sample out of 30 households that were interviewed 13 people belonged to Participants and 17 people belonged to non-Participants. The results from this study show that women participate a lot in farming activities. The multiple regression model was used to test the participation rates of the people in Amathole region specifically Phumlani area. A number of variables were considered in this study to assess the impact of different variables on participation in farming activities. The results showed that about 57% of the respondents are not participating in farming while 43% of the respondents participate. The farming participants that were interviewed all claim that there is a lack in farming support in the area. When there is no support of any kind, rural people would not be motivated to start development projects on their own. Consequently, this lack of farming support in the Phumlani area may have an influence on the number of farming participants. Therefore, the lack of support in the area may serve as a motivation for non-participants not to be influenced to farm. Rural farming needs to be promoted amongst the youth so as to protect and sustain agricultural growth in rural areas. The study has discovered that the youth of Phumlani is not actively involved in farming activities. Government can provide community members with farming resources so as to promote farming in the area. It would be wiser for the government to provide physical farming resources and implements rather than cash grants.
- Full Text:
- Date Issued: 2015
Factors affecting the implementation of HIV/AIDS awareness programmes in universities: a case study of selected university in Eastern Cape Province - South Africa
- Authors: Ibrahim, Seriki Idowu
- Date: 2015
- Subjects: AIDS (Disease) -- South Africa -- Eastern Cape , AIDS (Disease) -- Government policy -- South Africa -- Eastern Cape , Health promotion -- South Africa -- Eastern Cape , AIDS (Disease) -- South Africa -- Prevention
- Language: English
- Type: Thesis , Masters , MAdmin
- Identifier: http://hdl.handle.net/10353/1818 , vital:27562
- Description: Many studies have been carried out on Human Immunodeficiency Virus (HIV) and Acquired Immune Deficiency Syndrome (AIDS). Despite these giant stride (HIV) and (AIDS) have remained a great threat to human existence across the globe. Studies have shown that South Africa has the largest number of people with (HIV) and (AIDS) in Southern Africa. This study examined the factors influencing the implementation of (HIV) and (AIDS) A quantitative research design was adopted in the study. Two hundred and fifty nine subjects were involved in the study (259) this consisted of the University of Fort Hare Alice Campus in Eastern Cape Province. Five hypotheses were generated and tested in the study at 0.05 alpha levels. This study made use of descriptive and hypothesis testing analysis. Descriptive statistics such as the mean, mode, median and the frequency distribution graphs were also used. The study also revealed that there are factors influencing the implementation of HIV/AIDS awareness Programmes at the university of Fort Hare Alice campus F = 9.233, df = 4, 247 and p-value = 0.000, The University of Fort Hare do effectively implement HIV/AIDS policies and programmes. F = 10.765, df = 4, 247 and p-value = 0.000 . There are factors affecting the implementation of HIV/AIDS policies and programmes at university of Fort Hare Alice campus F = 2.207, df = 4, 247 and p-value = 0.069, There are significant difference on the factors affecting the implementation of HIV/AIDS policies and programmes within university Fort Hare Alice campus Chi-square statistic test: = 84.035, df= 16 and p-value = 0.000 (Pearson Chi-square), There are ways to improving the implementation of HIV/AIDS policies and programmes in university of Fort Hare Alice campus. F = 9.233, df = 4, 247 and p-value = 0.000, However, this study recommended that there is need to review the existing policy on (HIV) and (AIDS), the incorporation of (HIV) and (AIDS) awareness programme in the first year curriculum.
- Full Text:
- Date Issued: 2015
- Authors: Ibrahim, Seriki Idowu
- Date: 2015
- Subjects: AIDS (Disease) -- South Africa -- Eastern Cape , AIDS (Disease) -- Government policy -- South Africa -- Eastern Cape , Health promotion -- South Africa -- Eastern Cape , AIDS (Disease) -- South Africa -- Prevention
- Language: English
- Type: Thesis , Masters , MAdmin
- Identifier: http://hdl.handle.net/10353/1818 , vital:27562
- Description: Many studies have been carried out on Human Immunodeficiency Virus (HIV) and Acquired Immune Deficiency Syndrome (AIDS). Despite these giant stride (HIV) and (AIDS) have remained a great threat to human existence across the globe. Studies have shown that South Africa has the largest number of people with (HIV) and (AIDS) in Southern Africa. This study examined the factors influencing the implementation of (HIV) and (AIDS) A quantitative research design was adopted in the study. Two hundred and fifty nine subjects were involved in the study (259) this consisted of the University of Fort Hare Alice Campus in Eastern Cape Province. Five hypotheses were generated and tested in the study at 0.05 alpha levels. This study made use of descriptive and hypothesis testing analysis. Descriptive statistics such as the mean, mode, median and the frequency distribution graphs were also used. The study also revealed that there are factors influencing the implementation of HIV/AIDS awareness Programmes at the university of Fort Hare Alice campus F = 9.233, df = 4, 247 and p-value = 0.000, The University of Fort Hare do effectively implement HIV/AIDS policies and programmes. F = 10.765, df = 4, 247 and p-value = 0.000 . There are factors affecting the implementation of HIV/AIDS policies and programmes at university of Fort Hare Alice campus F = 2.207, df = 4, 247 and p-value = 0.069, There are significant difference on the factors affecting the implementation of HIV/AIDS policies and programmes within university Fort Hare Alice campus Chi-square statistic test: = 84.035, df= 16 and p-value = 0.000 (Pearson Chi-square), There are ways to improving the implementation of HIV/AIDS policies and programmes in university of Fort Hare Alice campus. F = 9.233, df = 4, 247 and p-value = 0.000, However, this study recommended that there is need to review the existing policy on (HIV) and (AIDS), the incorporation of (HIV) and (AIDS) awareness programme in the first year curriculum.
- Full Text:
- Date Issued: 2015
Factors enhancing readmission of mental health care users to a mental health unit in East London as described by their caregivers
- Authors: Mahashe, Nozizwe Patricia
- Date: 2015
- Language: English
- Type: Thesis , Masters , MSc (Psychiatric Nursing Science)
- Identifier: vital:11925 , http://hdl.handle.net/10353/d1021067
- Description: Readmission of mental healthcare users is a global challenge and concern which is associated with high costs. Readmission has negative effects as it is believed to promote dependency, demoralize patients and cause workload for staff. Re-admissions also bring a burden to caregivers and affect the caregiver’s health. In the light of this background, the aim of the study was to explore and describe factors enhancing readmission of mental health care users (MHCUs) to a mental health unit in East London as described by their caregivers. The objectives of the study were to: explore factors enhancing readmission of mental health care users in a mental health unit in East London as described by their caregivers and to document recommendations elicited from the findings of the study. A qualitative approach was used to collect and analyze data. The study used a descriptive and explorative design. Sampling was done using the purposive non-probability method. Unstructured face- to- face interviews were conducted using an interview guide. Fifteen (15) caregivers of MHCUs who had been readmitted to a mental health unit in East London were interviewed .The interviews were recorded and transcribed verbatim. The findings of this study suggest that non adherence to treatment, refusing to take treatment, defaulting treatment, denial, indulging in substance abuse, stigma, effects of disease, violence and aggressive behavior are the factors enhancing readmission of MCHUs to a mental health unit in East London. A better understanding of readmission of MHCUs can lead to early intervention and early planning of care. It is envisaged that psycho education, home visits and collaboration of clinic staff with hospital staff may decrease readmission of mental health care users in this unit.
- Full Text:
- Date Issued: 2015
- Authors: Mahashe, Nozizwe Patricia
- Date: 2015
- Language: English
- Type: Thesis , Masters , MSc (Psychiatric Nursing Science)
- Identifier: vital:11925 , http://hdl.handle.net/10353/d1021067
- Description: Readmission of mental healthcare users is a global challenge and concern which is associated with high costs. Readmission has negative effects as it is believed to promote dependency, demoralize patients and cause workload for staff. Re-admissions also bring a burden to caregivers and affect the caregiver’s health. In the light of this background, the aim of the study was to explore and describe factors enhancing readmission of mental health care users (MHCUs) to a mental health unit in East London as described by their caregivers. The objectives of the study were to: explore factors enhancing readmission of mental health care users in a mental health unit in East London as described by their caregivers and to document recommendations elicited from the findings of the study. A qualitative approach was used to collect and analyze data. The study used a descriptive and explorative design. Sampling was done using the purposive non-probability method. Unstructured face- to- face interviews were conducted using an interview guide. Fifteen (15) caregivers of MHCUs who had been readmitted to a mental health unit in East London were interviewed .The interviews were recorded and transcribed verbatim. The findings of this study suggest that non adherence to treatment, refusing to take treatment, defaulting treatment, denial, indulging in substance abuse, stigma, effects of disease, violence and aggressive behavior are the factors enhancing readmission of MCHUs to a mental health unit in East London. A better understanding of readmission of MHCUs can lead to early intervention and early planning of care. It is envisaged that psycho education, home visits and collaboration of clinic staff with hospital staff may decrease readmission of mental health care users in this unit.
- Full Text:
- Date Issued: 2015
Film as an agent of cultural transmission : a study of Nollywood films
- Onyenankeya, Oluwayemisi Mary
- Authors: Onyenankeya, Oluwayemisi Mary
- Date: 2015
- Subjects: Video recordings--Social aspects--Nigeria Motion pictures--Africa, West Digital video--Production and direction--Nigeria
- Language: English
- Type: Thesis , Masters , Communication
- Identifier: http://hdl.handle.net/10353/11685 , vital:39096
- Description: The popularity and viewership of Nollywood films have expanded across African countries as well as European and American cities especially those with considerable Diaspora population. This appears to underscore the assumption that Nollywood films not only project or transmit the embedded Nigerian cultural values to audience members but also actually make appreciable impression on those audience members. This study sought to ascertain the role of Nollywood film in the transference of Nigerian cultural heritage across other cultures using students at the University of Fort Hare as a case study. This study sought to determine if audience members learn, imbibe or manifest any behavioural tendencies that can be ascribed to the influence of watching Nollywood films. Specifically the study sought to establish the aspect of Nigerian culture that Nollywood films transmit to audience members. The survey research method was used while data was analysed using descriptive statistics. In all, 400 questionnaires were distributed while 370 were returned. Results show that students who were exposed to Nollywood film could mimic some common Nigerian expressions and exclamations. A small percentage of viewers could actually speak a smattering of some Nigerian language while a few have bought or made dresses in the pattern of what they saw in the films. It could be concluded that Nollywood films do exert significant influence on the viewers in the University of Fort Hare.
- Full Text:
- Date Issued: 2015
- Authors: Onyenankeya, Oluwayemisi Mary
- Date: 2015
- Subjects: Video recordings--Social aspects--Nigeria Motion pictures--Africa, West Digital video--Production and direction--Nigeria
- Language: English
- Type: Thesis , Masters , Communication
- Identifier: http://hdl.handle.net/10353/11685 , vital:39096
- Description: The popularity and viewership of Nollywood films have expanded across African countries as well as European and American cities especially those with considerable Diaspora population. This appears to underscore the assumption that Nollywood films not only project or transmit the embedded Nigerian cultural values to audience members but also actually make appreciable impression on those audience members. This study sought to ascertain the role of Nollywood film in the transference of Nigerian cultural heritage across other cultures using students at the University of Fort Hare as a case study. This study sought to determine if audience members learn, imbibe or manifest any behavioural tendencies that can be ascribed to the influence of watching Nollywood films. Specifically the study sought to establish the aspect of Nigerian culture that Nollywood films transmit to audience members. The survey research method was used while data was analysed using descriptive statistics. In all, 400 questionnaires were distributed while 370 were returned. Results show that students who were exposed to Nollywood film could mimic some common Nigerian expressions and exclamations. A small percentage of viewers could actually speak a smattering of some Nigerian language while a few have bought or made dresses in the pattern of what they saw in the films. It could be concluded that Nollywood films do exert significant influence on the viewers in the University of Fort Hare.
- Full Text:
- Date Issued: 2015
Geological and geophysical investigation of the South Eastern Karoo Basin, South Africa
- Authors: Baiyegunhi, Christopher
- Date: 2015
- Language: English
- Type: Thesis , Masters , MSc (Geology)
- Identifier: vital:11532 , http://hdl.handle.net/10353/d1021280
- Description: Geological and geophysical methods were used to investigate the southeastern Karoo Basin of South Africa in an area extending from longitudes 24 o E to 29o E and latitudes 32o S to 35o S. This was undertaken in order to reveal geologic structures, isochore thicknesses of the geologic sequence and their variations across the study area, proffer the possible provenance of the sediments and assess the potential of oil and gas accumulation. The methodology used includes field investigation, rock sampling, preparation of thin sections, petrographic studies, X-ray diffraction analysis, density measurements, porosity calculations, extraction of elevation data from Google Earth, 2½ D gravity profile modelling, generating of isochore (true vertical) thicknesses and depositional surfaces maps from the models, PetroMod 1D modelling and qualitative interpretation of magnetic, gravity and radiometric maps.Petrographic study was carried out on twenty six thin sections of rocks from the eleven geologic formations that cover the study area. Petrographic studies on the diamictite of the Dwyka Group shows abundance of monocrystalline quartz, granite and quartzite components in the breccias which possibly indicate the existence of granitic and metamorphic rocks in the source areas. The sandstones of the Ecca and Beaufort Groups are immature, greywacke and the heavy mineral assemblages signify that the minerals are of granitic, volcanic and metamorphic origin. The magnetic maps show two main magnetic anomalies, a major one trending in a northeast to southwest direction which is part of the Beattie magnetic anomaly and another that is a “bean-shaped” anomaly. The radially averaged power spectrum shows two depths to magnetic sources. The first depth is about 0.6 km which is the average depth to the top of the shallow sources, while the average depth to the top of the deep sources is about 15 km. The shallow sources are connected to magnetic minerals within the Beaufort Group while deep magnetic sources were inferred to be in the basement. The gamma ray spectrometric map shows areas with relatively high gamma radiation count. The high radiation count is possibly due to the uranium and thorium in the detrital materials, as well as the enrichment of radioelements in the feldspars (k-feldspar), calcite, quartz, zircon and clay minerals in the fluvial channel sandstones of the Beaufort Group. A total of two hundred and fifty-eight (258) rock samples were collected in the field and densities (dry, wet and grain densities) and porosities were determined in the laboratory. The Karoo Supergroup density values range from 2.526 – 2.828 g/cm3. The average porosities range from 0. 49 – 3.31 %. The dry densities and porosities of all the formations are inversely correlated with correlation coefficient values (R) that range from 0.9491 - 0.9982. The density of the dolerite intrusions (mostly sill) ranges from 2.700 – 2.837 g/cm3 whilst the porosity range from 0.1118 – 0.3868 %. The Bouguer anomaly map shows an increase in gravity values from -140.7 mGal in inland to about 60.1 mGal in coastal areas. This dominant gravity variation is inferred to be due to a deeper basement and/or Moho that get shallower from inland towards the coast. The Moho is at about 45 km depth inland and shallows to about 42 km at the coast. The 2½ D gravity modelling was done for fourteen (14) profiles with each profile having three (3) models corresponding to minimum, average and maximum densities to obtain the thicknesses of the geologic sequence. The current isochore thicknesses extracted from the gravity models show that the Beaufort Group is the thickest of all the groups that make up the Karoo Supergroup with maximum vertical thickness of up to 634 m, followed by the Ecca and Dwyka Groups with maximum vertical thicknesses of about 3207 m and 727 m, respectively. The maximum elevation for the Dwyka, Ecca and Beaufort sediments are about 500 m, 400 m and 285 m, respectively, whilst the maximum depth below sea level are around 8500 m, 7000 m and 5500 m, respectively. The PetroMod1D model result yield average vitrinite reflectance and temperature values of about 6 % and 500 ℃ respectively for the lower Ecca Group which belong to the dry gas window based on classification by several authors. Thus the rocks of the lower Ecca Group are thermally matured for hydrocarbon (shale gas) generation that can merit gas exploration in the Karoo Basin.
- Full Text:
- Date Issued: 2015
- Authors: Baiyegunhi, Christopher
- Date: 2015
- Language: English
- Type: Thesis , Masters , MSc (Geology)
- Identifier: vital:11532 , http://hdl.handle.net/10353/d1021280
- Description: Geological and geophysical methods were used to investigate the southeastern Karoo Basin of South Africa in an area extending from longitudes 24 o E to 29o E and latitudes 32o S to 35o S. This was undertaken in order to reveal geologic structures, isochore thicknesses of the geologic sequence and their variations across the study area, proffer the possible provenance of the sediments and assess the potential of oil and gas accumulation. The methodology used includes field investigation, rock sampling, preparation of thin sections, petrographic studies, X-ray diffraction analysis, density measurements, porosity calculations, extraction of elevation data from Google Earth, 2½ D gravity profile modelling, generating of isochore (true vertical) thicknesses and depositional surfaces maps from the models, PetroMod 1D modelling and qualitative interpretation of magnetic, gravity and radiometric maps.Petrographic study was carried out on twenty six thin sections of rocks from the eleven geologic formations that cover the study area. Petrographic studies on the diamictite of the Dwyka Group shows abundance of monocrystalline quartz, granite and quartzite components in the breccias which possibly indicate the existence of granitic and metamorphic rocks in the source areas. The sandstones of the Ecca and Beaufort Groups are immature, greywacke and the heavy mineral assemblages signify that the minerals are of granitic, volcanic and metamorphic origin. The magnetic maps show two main magnetic anomalies, a major one trending in a northeast to southwest direction which is part of the Beattie magnetic anomaly and another that is a “bean-shaped” anomaly. The radially averaged power spectrum shows two depths to magnetic sources. The first depth is about 0.6 km which is the average depth to the top of the shallow sources, while the average depth to the top of the deep sources is about 15 km. The shallow sources are connected to magnetic minerals within the Beaufort Group while deep magnetic sources were inferred to be in the basement. The gamma ray spectrometric map shows areas with relatively high gamma radiation count. The high radiation count is possibly due to the uranium and thorium in the detrital materials, as well as the enrichment of radioelements in the feldspars (k-feldspar), calcite, quartz, zircon and clay minerals in the fluvial channel sandstones of the Beaufort Group. A total of two hundred and fifty-eight (258) rock samples were collected in the field and densities (dry, wet and grain densities) and porosities were determined in the laboratory. The Karoo Supergroup density values range from 2.526 – 2.828 g/cm3. The average porosities range from 0. 49 – 3.31 %. The dry densities and porosities of all the formations are inversely correlated with correlation coefficient values (R) that range from 0.9491 - 0.9982. The density of the dolerite intrusions (mostly sill) ranges from 2.700 – 2.837 g/cm3 whilst the porosity range from 0.1118 – 0.3868 %. The Bouguer anomaly map shows an increase in gravity values from -140.7 mGal in inland to about 60.1 mGal in coastal areas. This dominant gravity variation is inferred to be due to a deeper basement and/or Moho that get shallower from inland towards the coast. The Moho is at about 45 km depth inland and shallows to about 42 km at the coast. The 2½ D gravity modelling was done for fourteen (14) profiles with each profile having three (3) models corresponding to minimum, average and maximum densities to obtain the thicknesses of the geologic sequence. The current isochore thicknesses extracted from the gravity models show that the Beaufort Group is the thickest of all the groups that make up the Karoo Supergroup with maximum vertical thickness of up to 634 m, followed by the Ecca and Dwyka Groups with maximum vertical thicknesses of about 3207 m and 727 m, respectively. The maximum elevation for the Dwyka, Ecca and Beaufort sediments are about 500 m, 400 m and 285 m, respectively, whilst the maximum depth below sea level are around 8500 m, 7000 m and 5500 m, respectively. The PetroMod1D model result yield average vitrinite reflectance and temperature values of about 6 % and 500 ℃ respectively for the lower Ecca Group which belong to the dry gas window based on classification by several authors. Thus the rocks of the lower Ecca Group are thermally matured for hydrocarbon (shale gas) generation that can merit gas exploration in the Karoo Basin.
- Full Text:
- Date Issued: 2015
Glycolytic potential and meat quality from dorper and merino sheep slaughtered at a commercial abattoir
- Authors: Stempa, Thuthuzelwa
- Date: 2015
- Subjects: Slaughtering and slaughter-houses Meat -- Quality
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/15310 , vital:40361
- Description: The objective of the study was to determine glycolytic potential and meat quality from Dorper and Merino sheep of both sexes slaughtered at a commercial abattoir. Dorper (n=52) and Merino (n= 48) breeds aged eight years, consisting of 50 intact rams and 50 non-pregnant ewes were used in the study. The sheep used in the study were reared, transported and lairised under identical conditions. Blood samples were collected at exsanguination for the measurement of glucose, lactate and cortisol levels. Samples were also collected from the Muscularis longmissius thoracis et lumborum (LTL) for the measurement glycogen, lactate levels, pH decline and colour. Correlations amongst blood stress indicators, muscle metabolites and meat quality attributes were also determined. Sex and breed had no effect on muscle glycolytic potential, glycogen and lactate levels from Dorper and Merino sheep of both sexes at the abattoir. Although sex and breed had an effect on pre-slaughter stress indicators (lactate and cortisol) collected at exsanguination. Ewes had higher levels of blood lactate (7.4 3 ± 0.49 mmol/L) and cortisol (293.92 ± 14.32 nmol/L) than the rams which had (5.19 ± 0.49 mmol/L) and (179.50 ± 14.32 nmol/L) lactate and cortisol levels, respectively. Furthermore, higher levels of lactate were observed in Dorper (7.54 ± 0.42 mmol/L) compared to the Merino sheep (4.97 ± 0.49 mmol/L). Meat pH decline and colour were also significantly affected by sex and breed. Ewes had higher levels of at pH45 minutes post slaughter (7.05 ± 0.04), pH3 hours (6.45 ± 0.04) , pH24 hours (6.00 ± 0.03), a* (14.31 ± 0.33) , b* (8.84 ± 0.29), H* (31.47 ± 0.73) and C* (16.75 ± 0.24) compared to the rams which had pH45 minutes (6.44 ± 0.04), pH3 hours (6.12 ± 0.04), pH24 hours (5.88 ± 0.03), a* (12.25 ± 0.33), b* (7.00 ± 0.29), H* (29.36 ± 0.73) and C* (14.15 ± 0.42) values. Moreover, Merino sheep had higher levels of L* (38.17 ± 0.48) and H* (31.59 ± 0.74) compared to the Dorper which had (36.39 ± 0.46) and (29.33 ± 0.71) L* and H* values, respectively. Blood cortisol was also positively correlated (P < 0.05) to glucose (r = 0.27), lactate (r = 0.37) but was negatively correlated (P < 0.001) to meat lightness (r = -0.44). Furthermore, blood cortisol was positively correlated (P < 0.001) to pH45 (r = 0.34), pH24 (r =0.22), meat yellowness (r = 0.24) and chroma (r = 0.37), but was negatively correlated to meat lightness (r = -0.47). Glycolytic potential was positively correlated (P < 0.001) to muscle glycogen levels (r = 0.66) and muscle lactate (r = 0.71).
- Full Text:
- Date Issued: 2015
- Authors: Stempa, Thuthuzelwa
- Date: 2015
- Subjects: Slaughtering and slaughter-houses Meat -- Quality
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/15310 , vital:40361
- Description: The objective of the study was to determine glycolytic potential and meat quality from Dorper and Merino sheep of both sexes slaughtered at a commercial abattoir. Dorper (n=52) and Merino (n= 48) breeds aged eight years, consisting of 50 intact rams and 50 non-pregnant ewes were used in the study. The sheep used in the study were reared, transported and lairised under identical conditions. Blood samples were collected at exsanguination for the measurement of glucose, lactate and cortisol levels. Samples were also collected from the Muscularis longmissius thoracis et lumborum (LTL) for the measurement glycogen, lactate levels, pH decline and colour. Correlations amongst blood stress indicators, muscle metabolites and meat quality attributes were also determined. Sex and breed had no effect on muscle glycolytic potential, glycogen and lactate levels from Dorper and Merino sheep of both sexes at the abattoir. Although sex and breed had an effect on pre-slaughter stress indicators (lactate and cortisol) collected at exsanguination. Ewes had higher levels of blood lactate (7.4 3 ± 0.49 mmol/L) and cortisol (293.92 ± 14.32 nmol/L) than the rams which had (5.19 ± 0.49 mmol/L) and (179.50 ± 14.32 nmol/L) lactate and cortisol levels, respectively. Furthermore, higher levels of lactate were observed in Dorper (7.54 ± 0.42 mmol/L) compared to the Merino sheep (4.97 ± 0.49 mmol/L). Meat pH decline and colour were also significantly affected by sex and breed. Ewes had higher levels of at pH45 minutes post slaughter (7.05 ± 0.04), pH3 hours (6.45 ± 0.04) , pH24 hours (6.00 ± 0.03), a* (14.31 ± 0.33) , b* (8.84 ± 0.29), H* (31.47 ± 0.73) and C* (16.75 ± 0.24) compared to the rams which had pH45 minutes (6.44 ± 0.04), pH3 hours (6.12 ± 0.04), pH24 hours (5.88 ± 0.03), a* (12.25 ± 0.33), b* (7.00 ± 0.29), H* (29.36 ± 0.73) and C* (14.15 ± 0.42) values. Moreover, Merino sheep had higher levels of L* (38.17 ± 0.48) and H* (31.59 ± 0.74) compared to the Dorper which had (36.39 ± 0.46) and (29.33 ± 0.71) L* and H* values, respectively. Blood cortisol was also positively correlated (P < 0.05) to glucose (r = 0.27), lactate (r = 0.37) but was negatively correlated (P < 0.001) to meat lightness (r = -0.44). Furthermore, blood cortisol was positively correlated (P < 0.001) to pH45 (r = 0.34), pH24 (r =0.22), meat yellowness (r = 0.24) and chroma (r = 0.37), but was negatively correlated to meat lightness (r = -0.47). Glycolytic potential was positively correlated (P < 0.001) to muscle glycogen levels (r = 0.66) and muscle lactate (r = 0.71).
- Full Text:
- Date Issued: 2015
Identification and documentation of ethnobiological methods used by rural farmers to control stalk borers on maize in the Eastern Cape Province of South Africa
- Authors: Skenjana, Nolitha Leonora
- Date: 2015
- Subjects: Ethnobotany , Stem borers , Biological pest control agents -- South Africa , Corn -- Diseases and pests -- South Africa
- Language: English
- Type: Thesis , Masters , MSc (Ethnobotany)
- Identifier: vital:11312 , http://hdl.handle.net/10353/d1019852 , Ethnobotany , Stem borers , Biological pest control agents -- South Africa , Corn -- Diseases and pests -- South Africa
- Description: Maize contributes substantially to food security in the Eastern Cape province of South Africa. It is a staple food to many of the province’s rural and urban inhabitants. Insect pests are one of the factors that hamper its productivity and as a result, deprive farmers of good yields. The adverse effects of insecticides and the high cost associated with them and the cost of transgenic seeds are some of the challenges faced by small-scale farmers in rural areas. Alternative control methods which include the use of indigenous techniques to control pests are now sought. A study to identify and document ethnobiological means used by rural farmers to manage insect pests of maize was conducted in the rural areas of the Eastern Cape Province. A total of 217 participants were interviewed on the matter, using semi-structured but detailed questionnaires. Rural farmers due to their linkage to agriculture activities and the fact that they are considered as custodians of agricultural indigenous knowledge were selected as respondents. Only maize producing and IsiXhosa speaking people were chosen to contribute. Main focus was on the demography of respondents, crop production activities and insect pest control. Pretesting of the questionnaire in order to assess the appropriateness of questions and comprehension by both farmers and enumerators was done. Data was analysed using descriptive statistics. Fifty five percent (55 %) of the respondents were females and the highest number of participants was from the Chris Hani District Municipality. Majority of the people were unemployed or pensioners. Most had only attended primary school and the mean age was 59 years. Apart from maize, respondents were cultivating other crops such as cabbage, Swiss chard, potatoes etc. Stalk borers followed by cutworms were the main pests of maize in these areas. Respondents used mainly insecticides, followed by alternative substances, which also included cultural control methods such as planting date manipulation. Few respondents used plants as control agents for insect pests. Some people did not control pests even though they were a problem in their fields. The most used plant was Chenopodium ambrosiodes L, while the most used substance was Madubula (a detergent). The most used insecticide was carbaryl from the carbamite family. Respondents listed different preparation techniques for all the control methods mentioned. These techniques revealed different times of preparation, quantities of ingredients, amounts applied on plants, modes of application and intervals of application. Rural farmers in the study areas used different atypical methods which may play a significant role in pest management today. Some of the products may have a positive influence on agriculture, while some are dangerous to humans and environmental health. Further research which will investigate their potential use in pest control needs to be done.
- Full Text:
- Date Issued: 2015
- Authors: Skenjana, Nolitha Leonora
- Date: 2015
- Subjects: Ethnobotany , Stem borers , Biological pest control agents -- South Africa , Corn -- Diseases and pests -- South Africa
- Language: English
- Type: Thesis , Masters , MSc (Ethnobotany)
- Identifier: vital:11312 , http://hdl.handle.net/10353/d1019852 , Ethnobotany , Stem borers , Biological pest control agents -- South Africa , Corn -- Diseases and pests -- South Africa
- Description: Maize contributes substantially to food security in the Eastern Cape province of South Africa. It is a staple food to many of the province’s rural and urban inhabitants. Insect pests are one of the factors that hamper its productivity and as a result, deprive farmers of good yields. The adverse effects of insecticides and the high cost associated with them and the cost of transgenic seeds are some of the challenges faced by small-scale farmers in rural areas. Alternative control methods which include the use of indigenous techniques to control pests are now sought. A study to identify and document ethnobiological means used by rural farmers to manage insect pests of maize was conducted in the rural areas of the Eastern Cape Province. A total of 217 participants were interviewed on the matter, using semi-structured but detailed questionnaires. Rural farmers due to their linkage to agriculture activities and the fact that they are considered as custodians of agricultural indigenous knowledge were selected as respondents. Only maize producing and IsiXhosa speaking people were chosen to contribute. Main focus was on the demography of respondents, crop production activities and insect pest control. Pretesting of the questionnaire in order to assess the appropriateness of questions and comprehension by both farmers and enumerators was done. Data was analysed using descriptive statistics. Fifty five percent (55 %) of the respondents were females and the highest number of participants was from the Chris Hani District Municipality. Majority of the people were unemployed or pensioners. Most had only attended primary school and the mean age was 59 years. Apart from maize, respondents were cultivating other crops such as cabbage, Swiss chard, potatoes etc. Stalk borers followed by cutworms were the main pests of maize in these areas. Respondents used mainly insecticides, followed by alternative substances, which also included cultural control methods such as planting date manipulation. Few respondents used plants as control agents for insect pests. Some people did not control pests even though they were a problem in their fields. The most used plant was Chenopodium ambrosiodes L, while the most used substance was Madubula (a detergent). The most used insecticide was carbaryl from the carbamite family. Respondents listed different preparation techniques for all the control methods mentioned. These techniques revealed different times of preparation, quantities of ingredients, amounts applied on plants, modes of application and intervals of application. Rural farmers in the study areas used different atypical methods which may play a significant role in pest management today. Some of the products may have a positive influence on agriculture, while some are dangerous to humans and environmental health. Further research which will investigate their potential use in pest control needs to be done.
- Full Text:
- Date Issued: 2015
Identification of agricultural and industrial pollutants in the Kat River, Eastern Cape and their effect on agricultural products found along the river banks
- Authors: Mutingwende, Nhamo
- Date: 2015
- Subjects: Environmental toxicology , Rivers -- Environmental aspects -- South Africa , Water -- Pollution -- Toxicology -- South Africa
- Language: English
- Type: Thesis , Masters , MSc (Biochemistry)
- Identifier: vital:11291 , http://hdl.handle.net/10353/d1020242 , Environmental toxicology , Rivers -- Environmental aspects -- South Africa , Water -- Pollution -- Toxicology -- South Africa
- Description: There is growing concern that commonly used Pharmaceuticals and Personal Care Products (PPCPs) and pesticides are entering and contaminating drinking water supplies. The use of targeted quantitation of PPCP has been well established but there is an emerging trend to also screen for and identify unexpected environmental pollutants. Chemicals like pesticides hormones and antibiotics are especially of interest because of proven endocrine disrupting effects and a possible development of bacterial resistance. Powerful screening methods are required to detect and quantify the presence of these compounds in our environment. PPCP encompass a wide range of pollutants, including Endocrine Disrupting Compounds (EDC), pesticides, hormones, antibiotics, drugs of abuse, x-ray contrast agents and drinking water disinfection by-products to name a few. In order to properly assess the effects of these compounds on our environment, it is necessary to accurately monitor their presence. The diversity of chemical properties of these compounds makes method development challenging. LC/MS/MS is able to analyse polar, semi-volatile, and thermally labile compounds covering a wide molecular weight range. The new AB SCIEX TripleTOF™5600 LC/MS/MS was used to profile environmental samples for unexpected pollutants, to identify and characterise the chemical composition and structure of the pollutants, and to quantify (based on intensity) the concentration in collected water samples. Liquid Chromatography coupled to tandem Mass Spectrometry (LCMS/ MS) is able to analyse polar, semi-volatile, and thermally labile compounds covering a wide molecular weight range, such as pesticides, antibiotics, drugs of abuse, x-ray contrast agents, drinking water disinfection by-products etc. More recently there is a growing interest from environmental researchers to also screen for and identify non-targeted compounds in environmental samples, including metabolites and degradates, but also completely unexpected pollutants. The new AB SCIEX TripleTOF™5600 LC/MS/MS system is capable of performing highly sensitive and fast MS scanning experiments to search for unknown molecular ions while also performing selective and characteristic MS/MS scanning for further compound identification and, therefore, is the instrument of choice for this challenging task. General unknown screening workflows do not use a target analyte list and compound detection is not based on any prior knowledge, including retention times and information on possible molecular and fragment ions. Therefore, acquired chromatograms are very rich in information and can easily contain thousands of ions from both any compounds present in the sample as well as from the sample matrix itself. Thus, powerful software tools are needed to explore such data to identify the unexpected compound. Water samples were collected both upstream and downstream of two WWTPs (Seymour and Fort Beaufort) and were directly injected on the AB SCIEX TripleTOF™5600 LC/MS/MS after being filtered. 15 sample points along the Kat River, ranging from a point as close to the source as possible to a point just before it joins the Great Fish River were used. The samples collected from the source were used as the control in each of the experiments, the assumption being the closer you get to the source, the less contaminated the water would be for the analysis of pesticides. Points were selected where the Kat River crosses the R67 or on farms where the river was accessible using farm roads. Samples were collected from October 2013 to November 2014.The Peak view software and Analyst software were used in the analysis of PPCPs. The XIC Manager allows you to manage large lists of compounds and perform automatic extracted ion chromatogram (XIC) calculations and review results operations. The results were displayed in the chromatogram pane and the XIC table (see results). The results reported here in this thesis indicate that there is contamination in the Kat River water due to both pesticides and PPCPs. The results also indicate that the food products are also contaminated and hence both the Kat River agricultural produce and its water need to be closely monitored for both pesticide and PPCPs contaminants. Further studies to investigate the quantitative levels of pesticides and PPCPs in the Kat river water to determine if the concentration levels of the detected pesticides are below the reported Maximum Residues Limits will be explored in the future.
- Full Text:
- Date Issued: 2015
- Authors: Mutingwende, Nhamo
- Date: 2015
- Subjects: Environmental toxicology , Rivers -- Environmental aspects -- South Africa , Water -- Pollution -- Toxicology -- South Africa
- Language: English
- Type: Thesis , Masters , MSc (Biochemistry)
- Identifier: vital:11291 , http://hdl.handle.net/10353/d1020242 , Environmental toxicology , Rivers -- Environmental aspects -- South Africa , Water -- Pollution -- Toxicology -- South Africa
- Description: There is growing concern that commonly used Pharmaceuticals and Personal Care Products (PPCPs) and pesticides are entering and contaminating drinking water supplies. The use of targeted quantitation of PPCP has been well established but there is an emerging trend to also screen for and identify unexpected environmental pollutants. Chemicals like pesticides hormones and antibiotics are especially of interest because of proven endocrine disrupting effects and a possible development of bacterial resistance. Powerful screening methods are required to detect and quantify the presence of these compounds in our environment. PPCP encompass a wide range of pollutants, including Endocrine Disrupting Compounds (EDC), pesticides, hormones, antibiotics, drugs of abuse, x-ray contrast agents and drinking water disinfection by-products to name a few. In order to properly assess the effects of these compounds on our environment, it is necessary to accurately monitor their presence. The diversity of chemical properties of these compounds makes method development challenging. LC/MS/MS is able to analyse polar, semi-volatile, and thermally labile compounds covering a wide molecular weight range. The new AB SCIEX TripleTOF™5600 LC/MS/MS was used to profile environmental samples for unexpected pollutants, to identify and characterise the chemical composition and structure of the pollutants, and to quantify (based on intensity) the concentration in collected water samples. Liquid Chromatography coupled to tandem Mass Spectrometry (LCMS/ MS) is able to analyse polar, semi-volatile, and thermally labile compounds covering a wide molecular weight range, such as pesticides, antibiotics, drugs of abuse, x-ray contrast agents, drinking water disinfection by-products etc. More recently there is a growing interest from environmental researchers to also screen for and identify non-targeted compounds in environmental samples, including metabolites and degradates, but also completely unexpected pollutants. The new AB SCIEX TripleTOF™5600 LC/MS/MS system is capable of performing highly sensitive and fast MS scanning experiments to search for unknown molecular ions while also performing selective and characteristic MS/MS scanning for further compound identification and, therefore, is the instrument of choice for this challenging task. General unknown screening workflows do not use a target analyte list and compound detection is not based on any prior knowledge, including retention times and information on possible molecular and fragment ions. Therefore, acquired chromatograms are very rich in information and can easily contain thousands of ions from both any compounds present in the sample as well as from the sample matrix itself. Thus, powerful software tools are needed to explore such data to identify the unexpected compound. Water samples were collected both upstream and downstream of two WWTPs (Seymour and Fort Beaufort) and were directly injected on the AB SCIEX TripleTOF™5600 LC/MS/MS after being filtered. 15 sample points along the Kat River, ranging from a point as close to the source as possible to a point just before it joins the Great Fish River were used. The samples collected from the source were used as the control in each of the experiments, the assumption being the closer you get to the source, the less contaminated the water would be for the analysis of pesticides. Points were selected where the Kat River crosses the R67 or on farms where the river was accessible using farm roads. Samples were collected from October 2013 to November 2014.The Peak view software and Analyst software were used in the analysis of PPCPs. The XIC Manager allows you to manage large lists of compounds and perform automatic extracted ion chromatogram (XIC) calculations and review results operations. The results were displayed in the chromatogram pane and the XIC table (see results). The results reported here in this thesis indicate that there is contamination in the Kat River water due to both pesticides and PPCPs. The results also indicate that the food products are also contaminated and hence both the Kat River agricultural produce and its water need to be closely monitored for both pesticide and PPCPs contaminants. Further studies to investigate the quantitative levels of pesticides and PPCPs in the Kat river water to determine if the concentration levels of the detected pesticides are below the reported Maximum Residues Limits will be explored in the future.
- Full Text:
- Date Issued: 2015
Information communication technology (ICT) community centres and agricultural development in the Eastern Cape province of South Africa: a case of Dwesa community
- Authors: Mukasi, Tafadzwa Jaquline
- Date: 2015
- Subjects: Information technology -- South Africa -- Eastern Cape Telecommunication -- South Africa -- Eastern Cape Agricultural innovations -- South Africa -- Eastern Cape Poverty -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , M Soc Sci
- Identifier: http://hdl.handle.net/10353/1686 , vital:27545
- Description: The development of Information Communication Technology (ICT) in rural areas has
- Full Text:
- Date Issued: 2015
- Authors: Mukasi, Tafadzwa Jaquline
- Date: 2015
- Subjects: Information technology -- South Africa -- Eastern Cape Telecommunication -- South Africa -- Eastern Cape Agricultural innovations -- South Africa -- Eastern Cape Poverty -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , M Soc Sci
- Identifier: http://hdl.handle.net/10353/1686 , vital:27545
- Description: The development of Information Communication Technology (ICT) in rural areas has
- Full Text:
- Date Issued: 2015
Investigating the factors affecting citizen's ability to access e-government services in Nkonkobe municipality in the Easterrn Cape province
- Authors: Ntobongwana,Nolitha
- Date: 2015
- Subjects: Internet in public administration -- South Africa -- Eastern Cape Electronic government information -- South Africa -- Eastern Cape Municipal services -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , M Admin
- Identifier: http://hdl.handle.net/10353/1708 , vital:27551
- Description: E-government which refers to electronic Government is the process of transformation of relationships of government with its constituents ie the citizens, the businesses and between its own organs, through the use of the tools of Information and Communications Technology (ICT). E-government services are the outcome of e-governance that come as automated services delivered through ICTs particularly Internet based applications that provide access to and delivery of information or services to the public, businesses, other agencies and governmental departments. This study seeks to examine the factors affecting citizens ability to access e-government services in Nkonkobe Municipality in the province of Eastern Cape and come with recommendations of how to address the issue. E-government services should be developed on platforms with the consideration of devices that people can have access to. The study showed that in as much as one can have cell phone that has access to internet , some website services such as home affairs enquiry applications are not accessible on a phone with a general web browser that has no Java Script capacity but has acces to internet. Therefore developing Short Message Service (SMS) based services or Unstructured Supplementary Serviuce Data (USSD) code that is available on all Globile System for Mobile (GSM) cell phones can increase accessibility of most services at a cheaper cost. The study concludes that although e-government is the future for our country and in this era of technology, much still has to be done in areas like Nkonkobe Municipality in Eastern Cape, as the citizens have no or lack appropriate tools to access the internet, and sometimes citizens are not aware of e-government services available. The research indicates that most citizens have cell phones despite the differences in handset capabilities, which therefore means that applications developed for e-government services can be ported foe accessibility to ordinary GSM cell phone including those without internet access.
- Full Text:
- Date Issued: 2015
- Authors: Ntobongwana,Nolitha
- Date: 2015
- Subjects: Internet in public administration -- South Africa -- Eastern Cape Electronic government information -- South Africa -- Eastern Cape Municipal services -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , M Admin
- Identifier: http://hdl.handle.net/10353/1708 , vital:27551
- Description: E-government which refers to electronic Government is the process of transformation of relationships of government with its constituents ie the citizens, the businesses and between its own organs, through the use of the tools of Information and Communications Technology (ICT). E-government services are the outcome of e-governance that come as automated services delivered through ICTs particularly Internet based applications that provide access to and delivery of information or services to the public, businesses, other agencies and governmental departments. This study seeks to examine the factors affecting citizens ability to access e-government services in Nkonkobe Municipality in the province of Eastern Cape and come with recommendations of how to address the issue. E-government services should be developed on platforms with the consideration of devices that people can have access to. The study showed that in as much as one can have cell phone that has access to internet , some website services such as home affairs enquiry applications are not accessible on a phone with a general web browser that has no Java Script capacity but has acces to internet. Therefore developing Short Message Service (SMS) based services or Unstructured Supplementary Serviuce Data (USSD) code that is available on all Globile System for Mobile (GSM) cell phones can increase accessibility of most services at a cheaper cost. The study concludes that although e-government is the future for our country and in this era of technology, much still has to be done in areas like Nkonkobe Municipality in Eastern Cape, as the citizens have no or lack appropriate tools to access the internet, and sometimes citizens are not aware of e-government services available. The research indicates that most citizens have cell phones despite the differences in handset capabilities, which therefore means that applications developed for e-government services can be ported foe accessibility to ordinary GSM cell phone including those without internet access.
- Full Text:
- Date Issued: 2015
Investigation into the characteristics and possible applications of biomass gasification by-products from a downdraft gasifier system
- Authors: Melapi, Aviwe
- Date: 2015
- Subjects: Biomass gasification -- South Africa -- Eastern Cape , Renewable energy sources -- South Africa -- Eastern Cape , Biomass energy -- South Africa -- Eastern Cape , Crop residue management -- South Africa -- Eastern Cape , Coal gasification -- South Africa -- Eastern Cape , Lignocellulose -- Biodegradation -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc (Chemistry)
- Identifier: vital:11348 , http://hdl.handle.net/10353/d1020174 , Biomass gasification -- South Africa -- Eastern Cape , Renewable energy sources -- South Africa -- Eastern Cape , Biomass energy -- South Africa -- Eastern Cape , Crop residue management -- South Africa -- Eastern Cape , Coal gasification -- South Africa -- Eastern Cape , Lignocellulose -- Biodegradation -- South Africa -- Eastern Cape
- Description: Biomass gasification has attracted the interest of researchers because it produces zero carbon to the atmosphere. This technology does not only produce syngas but also the byproducts which can be used for various application depending on quality.The study conducted at Melani village in Alice in the Eastern Cape of South Africa was aimed at investigating the possible applications of the gasification byproducts instead of being thrown away. Pine wood was employed as the parent feedstock material for the gasifier. Biomass gasification by-products were then collected for further analysis. The studied by-products included tar(condensate), char, soot and resin. These materials were also blended to produce strong materials.The essence of the blending was to generate ideal material that is strong but light at the same time.The elemental analysis of the samples performed by CHNS analyser revealed that carbon element is in large quantities in all samples. The FTIR spectra showed almost similar results for all the studied samples, since the samples are end products of lignocellulosegasification. SEM gave the sticky images of resin as well as porous char structures. Char showed a higher heating value of 35.37MJ/Kg when compared to other by-products samples.
- Full Text:
- Date Issued: 2015
- Authors: Melapi, Aviwe
- Date: 2015
- Subjects: Biomass gasification -- South Africa -- Eastern Cape , Renewable energy sources -- South Africa -- Eastern Cape , Biomass energy -- South Africa -- Eastern Cape , Crop residue management -- South Africa -- Eastern Cape , Coal gasification -- South Africa -- Eastern Cape , Lignocellulose -- Biodegradation -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc (Chemistry)
- Identifier: vital:11348 , http://hdl.handle.net/10353/d1020174 , Biomass gasification -- South Africa -- Eastern Cape , Renewable energy sources -- South Africa -- Eastern Cape , Biomass energy -- South Africa -- Eastern Cape , Crop residue management -- South Africa -- Eastern Cape , Coal gasification -- South Africa -- Eastern Cape , Lignocellulose -- Biodegradation -- South Africa -- Eastern Cape
- Description: Biomass gasification has attracted the interest of researchers because it produces zero carbon to the atmosphere. This technology does not only produce syngas but also the byproducts which can be used for various application depending on quality.The study conducted at Melani village in Alice in the Eastern Cape of South Africa was aimed at investigating the possible applications of the gasification byproducts instead of being thrown away. Pine wood was employed as the parent feedstock material for the gasifier. Biomass gasification by-products were then collected for further analysis. The studied by-products included tar(condensate), char, soot and resin. These materials were also blended to produce strong materials.The essence of the blending was to generate ideal material that is strong but light at the same time.The elemental analysis of the samples performed by CHNS analyser revealed that carbon element is in large quantities in all samples. The FTIR spectra showed almost similar results for all the studied samples, since the samples are end products of lignocellulosegasification. SEM gave the sticky images of resin as well as porous char structures. Char showed a higher heating value of 35.37MJ/Kg when compared to other by-products samples.
- Full Text:
- Date Issued: 2015
Isolation and characterisation of lignocellulose degrading bacteria from Tyume River in the Eastern Cape Province, South Africa
- Authors: Tembisa, Papiyana Ayavuya
- Date: 2015
- Subjects: Lignocellulose -- Biodegradation -- South Africa -- Eastern Cape , Bacillus (Bacteria) -- South Africa -- Eastern Cape , Waterborne infection -- South Africa -- Eastern Cape , Bacteriophages -- South Africa -- Eastern Cape , Sediments (Geology) -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc (Microbiology)
- Identifier: vital:11299 , http://hdl.handle.net/10353/d1021293 , Lignocellulose -- Biodegradation -- South Africa -- Eastern Cape , Bacillus (Bacteria) -- South Africa -- Eastern Cape , Waterborne infection -- South Africa -- Eastern Cape , Bacteriophages -- South Africa -- Eastern Cape , Sediments (Geology) -- South Africa -- Eastern Cape
- Description: This study focuses on the isolation and characterization of bacteria from lignocellulosic biomass obtained from the sediments of the Tyume River in Alice, Eastern Cape and to determine those bacterial isolates with good potential for modification and decomposition of lignocellulosic biomass for industrial application. Several bacterial isolates were recovered and screened for ability to degrade various lignocellulosic materials. Nine of the isolates were positive for lignocellulolytic activity. Four isolates were cellulase positive and six were xylanase positive. Moreover, one isolate (SB1) was positive for both xylanase and cellulase activities and showed the best hydrolysis zone on solid media. This isolate was then chosen as the best and identified molecularly. The 16S rDNA sequence analysis indicated that SB1 was a Bacillus cereus species. Factors affecting the cellulose and xylanase enzyme production by the organisms were studied. The organisms produced the enzymes maximally at earlier hours of incubation (12-30 hr) and optimally at acidic pH (3-5) and at moderate temperatures (35-45ºC). SB1 appears to hold promise in the decomposition of lignocellulosic wastes.
- Full Text:
- Date Issued: 2015
- Authors: Tembisa, Papiyana Ayavuya
- Date: 2015
- Subjects: Lignocellulose -- Biodegradation -- South Africa -- Eastern Cape , Bacillus (Bacteria) -- South Africa -- Eastern Cape , Waterborne infection -- South Africa -- Eastern Cape , Bacteriophages -- South Africa -- Eastern Cape , Sediments (Geology) -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc (Microbiology)
- Identifier: vital:11299 , http://hdl.handle.net/10353/d1021293 , Lignocellulose -- Biodegradation -- South Africa -- Eastern Cape , Bacillus (Bacteria) -- South Africa -- Eastern Cape , Waterborne infection -- South Africa -- Eastern Cape , Bacteriophages -- South Africa -- Eastern Cape , Sediments (Geology) -- South Africa -- Eastern Cape
- Description: This study focuses on the isolation and characterization of bacteria from lignocellulosic biomass obtained from the sediments of the Tyume River in Alice, Eastern Cape and to determine those bacterial isolates with good potential for modification and decomposition of lignocellulosic biomass for industrial application. Several bacterial isolates were recovered and screened for ability to degrade various lignocellulosic materials. Nine of the isolates were positive for lignocellulolytic activity. Four isolates were cellulase positive and six were xylanase positive. Moreover, one isolate (SB1) was positive for both xylanase and cellulase activities and showed the best hydrolysis zone on solid media. This isolate was then chosen as the best and identified molecularly. The 16S rDNA sequence analysis indicated that SB1 was a Bacillus cereus species. Factors affecting the cellulose and xylanase enzyme production by the organisms were studied. The organisms produced the enzymes maximally at earlier hours of incubation (12-30 hr) and optimally at acidic pH (3-5) and at moderate temperatures (35-45ºC). SB1 appears to hold promise in the decomposition of lignocellulosic wastes.
- Full Text:
- Date Issued: 2015
Isolation and molecular characterization of Bacillus cereus from cow’s raw milk
- Authors: Lukanji, Zinathi , Ndip, R N
- Date: 2015
- Subjects: Milk contamination -- South Africa -- Eastern Cape , Bacillus (Bacteria) -- South Africa -- Eastern Cape , Foodborne diseases -- Molecular diagnosis -- South Africa -- Eastern Cape , Dairy products -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc (Microbiology)
- Identifier: vital:11296 , http://hdl.handle.net/10353/d1021284 , Milk contamination -- South Africa -- Eastern Cape , Bacillus (Bacteria) -- South Africa -- Eastern Cape , Foodborne diseases -- Molecular diagnosis -- South Africa -- Eastern Cape , Dairy products -- South Africa -- Eastern Cape
- Description: Bacillus cereus is a group of ubiquitous facultative anaerobic spore forming Gram-positive rods commonly found in soil. It has been detected and implicated in several contaminated food products and raw milk in dairy farms and it causes foodborne gastroenteritis by producing several toxins. This study is aimed at characterizing virulence determinants of B. cereus from cow‟s raw milk. A total of 400 raw milk samples were collected in Fort Hare Dairy Trust and Middledrift Dairy Farm; and cultured on Polymyxin pyruvate Egg-Yolk Mannitol Bromothymol Agar (PEMBA) for 48 hours at 37°C. DNA was isolated from the isolates and 16S rDNA was amplified and sent to Central Analytical Laboratory for sequencing. The gyrB gene of B. cereus was also used to confirm the identity of the isolates. Antibiotic susceptibility profiles of the isolates together with virulence genes were investigated. Multilocus Sequence typing was used to investigate the genetic relatedness of some selected isolates. Furthermore, spores of the isolates were produced, harvested and their concentrations determined. All (100%) of the isolates were identified as having a 96-99% similarity to B. cereus, B. thuringiensis and B. anthracis using sequencing; while gyrB gene was observed in all (100%) of the isolates. Three virulence genes nheA, nheB, nheC encoding for non haemolysin enterotoxin were amplified in all (100%) the isolates. All (100%) of the isolates were susceptible to doxycycline, gentamycin, tetracycline, ciprofloxacin, chloramphenicol and streptomycin. Resistance to rifampicin and penicillin G was predominant with equal rate of 100%, while susceptibility to erythromycin, clindamycin and doxycycline ranged from 60% to 100%. The selected isolates were related and are descendants of the same ancestor. All (100%) the isolates produced spores. The B. cereus isolates contain virulence genes, has multiple antibiotic drug resistance and produce spores, which poses a health risk to the public and cannot be used as probiotics.
- Full Text:
- Date Issued: 2015
- Authors: Lukanji, Zinathi , Ndip, R N
- Date: 2015
- Subjects: Milk contamination -- South Africa -- Eastern Cape , Bacillus (Bacteria) -- South Africa -- Eastern Cape , Foodborne diseases -- Molecular diagnosis -- South Africa -- Eastern Cape , Dairy products -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc (Microbiology)
- Identifier: vital:11296 , http://hdl.handle.net/10353/d1021284 , Milk contamination -- South Africa -- Eastern Cape , Bacillus (Bacteria) -- South Africa -- Eastern Cape , Foodborne diseases -- Molecular diagnosis -- South Africa -- Eastern Cape , Dairy products -- South Africa -- Eastern Cape
- Description: Bacillus cereus is a group of ubiquitous facultative anaerobic spore forming Gram-positive rods commonly found in soil. It has been detected and implicated in several contaminated food products and raw milk in dairy farms and it causes foodborne gastroenteritis by producing several toxins. This study is aimed at characterizing virulence determinants of B. cereus from cow‟s raw milk. A total of 400 raw milk samples were collected in Fort Hare Dairy Trust and Middledrift Dairy Farm; and cultured on Polymyxin pyruvate Egg-Yolk Mannitol Bromothymol Agar (PEMBA) for 48 hours at 37°C. DNA was isolated from the isolates and 16S rDNA was amplified and sent to Central Analytical Laboratory for sequencing. The gyrB gene of B. cereus was also used to confirm the identity of the isolates. Antibiotic susceptibility profiles of the isolates together with virulence genes were investigated. Multilocus Sequence typing was used to investigate the genetic relatedness of some selected isolates. Furthermore, spores of the isolates were produced, harvested and their concentrations determined. All (100%) of the isolates were identified as having a 96-99% similarity to B. cereus, B. thuringiensis and B. anthracis using sequencing; while gyrB gene was observed in all (100%) of the isolates. Three virulence genes nheA, nheB, nheC encoding for non haemolysin enterotoxin were amplified in all (100%) the isolates. All (100%) of the isolates were susceptible to doxycycline, gentamycin, tetracycline, ciprofloxacin, chloramphenicol and streptomycin. Resistance to rifampicin and penicillin G was predominant with equal rate of 100%, while susceptibility to erythromycin, clindamycin and doxycycline ranged from 60% to 100%. The selected isolates were related and are descendants of the same ancestor. All (100%) the isolates produced spores. The B. cereus isolates contain virulence genes, has multiple antibiotic drug resistance and produce spores, which poses a health risk to the public and cannot be used as probiotics.
- Full Text:
- Date Issued: 2015
Land and agrarian reform, and rural livelihoods in post-apartheid South Africa : a study on the Ehlanzeni District in Mpumalanga Province
- Authors: Maduna-Mafu, Nqobani
- Date: 2015
- Subjects: Land reform -- South Africa -- Mpumalanga Land use, Rural -- South Africa -- Mpumalanga South Africa -- Economic conditions
- Language: English
- Type: Thesis , Masters , MSoc
- Identifier: http://hdl.handle.net/10353/4514 , vital:28346
- Description: The study examines land and agrarian reforms in democratic South Africa focusing on projects implemented under the Comprehensive Rural Development Programme (CRDP) since 2009. Focusing on Bushbuckridge municipal area, the study reviews wide ranging transformative efforts initiated to address agrarian sector inequalities and rural poverty. The review illustrates that modest achievements have been made in restructuring the apartheid political geography manifesting in high levels of asset poverty amongst rural populations and vulnerability to food insecurity. While several factors are explored to explain this, it is discernible that a disjuncture exists between the social justice imperatives and the neoliberal development ideology adopted since transition to democracy. Adopting the sustainable livelihoods approach, the study examines whether the CRDP is adequate to address the needs for land equity in redistribution and to improve rural livelihoods in Mpumalanga, with particular focus on Bushbuckridge municipal area. Furthermore, the study examines the extent to which the implementation of CRDP has met the objective of equitable land redistribution as specified in the policy and also explores the outcomes of implementation, whether necessary conditions to promote the small-scale agricultural sector have been created; for instance, investments in agricultural infrastructure, support services such as credit measures, inputs and capacity building programmes for subsistence sector farming. Through a qualitative inquiry challenges are identified regarding the achievement of equity in land distribution and sustainable livelihoods. The conclusive chapter argues for paradigm shifts in land acquisition, public engagement, gender equity, funding models for subsistence farming, intersectoral co-operation, funding of rural infrastructural projects particularly markets and agro-processing facilities.
- Full Text:
- Date Issued: 2015
- Authors: Maduna-Mafu, Nqobani
- Date: 2015
- Subjects: Land reform -- South Africa -- Mpumalanga Land use, Rural -- South Africa -- Mpumalanga South Africa -- Economic conditions
- Language: English
- Type: Thesis , Masters , MSoc
- Identifier: http://hdl.handle.net/10353/4514 , vital:28346
- Description: The study examines land and agrarian reforms in democratic South Africa focusing on projects implemented under the Comprehensive Rural Development Programme (CRDP) since 2009. Focusing on Bushbuckridge municipal area, the study reviews wide ranging transformative efforts initiated to address agrarian sector inequalities and rural poverty. The review illustrates that modest achievements have been made in restructuring the apartheid political geography manifesting in high levels of asset poverty amongst rural populations and vulnerability to food insecurity. While several factors are explored to explain this, it is discernible that a disjuncture exists between the social justice imperatives and the neoliberal development ideology adopted since transition to democracy. Adopting the sustainable livelihoods approach, the study examines whether the CRDP is adequate to address the needs for land equity in redistribution and to improve rural livelihoods in Mpumalanga, with particular focus on Bushbuckridge municipal area. Furthermore, the study examines the extent to which the implementation of CRDP has met the objective of equitable land redistribution as specified in the policy and also explores the outcomes of implementation, whether necessary conditions to promote the small-scale agricultural sector have been created; for instance, investments in agricultural infrastructure, support services such as credit measures, inputs and capacity building programmes for subsistence sector farming. Through a qualitative inquiry challenges are identified regarding the achievement of equity in land distribution and sustainable livelihoods. The conclusive chapter argues for paradigm shifts in land acquisition, public engagement, gender equity, funding models for subsistence farming, intersectoral co-operation, funding of rural infrastructural projects particularly markets and agro-processing facilities.
- Full Text:
- Date Issued: 2015