- Title
- Incidence and antibiogram fingerprints of members of the Enterobacteriaceae family recovered from river water, hospital effluents and vegetables in Chris Hani and Amathole District Municipalities in the Eastern Cape Province
- Creator
- Mpaka, Lindelwa
- Subject
- Enterobacteriaceae
- Date
- 2018
- Type
- Thesis
- Type
- Masters
- Type
- MSc (Microbiology)
- Identifier
- http://hdl.handle.net/10353/14549
- Identifier
- vital:40003
- Description
- The worldwide problem of antimicrobial resistance has limited the spectrum of the current affordable and effective antimicrobials. Infections associated with resistant microorganisms impose a major threat to public health and economic stability. Globally, about 700 000 deaths every year can be accredited to antimicrobial resistance. The leading mechanism of resistance amid bacterial pathogens is the extended spectrum beta-lactamases production, which inhibits spectrum activity of several antimicrobial agents. The rise in antimicrobial resistance has compelled an urgent need of developing means of combatting resistance issue amid diseasecausing microbes. The main aim of this study is to evaluate the incidence and antibiogram fingerprints of Enterobacteriaceae recovered from hospital effluents, river water and vegetables in the Eastern Cape Province. A total of eighteen antibiotics from ten different antimicrobial classes were used to determine antibiogram profiles of the MALDI-TOF confirmed isolates. From the MALDI-TOF confirmed isolates, 60% of Enterobacter spp. and E. coli isolates displayed resistance against colistin, while Citrobacter spp. and Klebsiella spp. displayed 90% and 60% resistance against this antimicrobial respectively. These findings outline the need for the development of new antimicrobials. About 75.5% (25/33) of the presumptive Enterobacter spp. were confirmed by MALDI-TOF with 79.2% (19/24), 66.7% (2/3), 66.7% (4/6) been confirmed vegetables, hospital effluents and river water samples respectively. Likewise, about 77.8% (21/27) were confirmed as Citrobacter spp. of which 92.3% (12/13), 66.7% (2/3) and 63.6% (7/11) were from vegetables, hospital effluents and river water samples respectively. These results show that the selected vegetables were highly contaminated with resistant bacteria and thus unsafe to consume uncooked vegetable. Also river water was higly contaminated with resistant microbes, which also shows that these rivers are not fit to be used 17 as drinking water sources and recreational activities. Colistin is an antimicrobial used as a last resort of antibiotics because it exhibits broad-spectrum activity. However from the findings of the work at present, this is no longer the case. The spectrum of this antimicrobial is now reduced by Enterobacteriaceae members. To the best of my knowledge; relatively few resources have been provided to understanding, preventing, and controlling increasing antimicrobial resistance on global, national and local levels.
- Format
- 213 leaves
- Format
- Publisher
- University of Fort Hare
- Publisher
- Faculty of Science and Agriculture
- Language
- English
- Rights
- University of Fort Hare
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UFH Department of Biochemistry & Microbiology
| Thumbnail | File | Description | Size | Format | |||
|---|---|---|---|---|---|---|---|
| View Details | SOURCE1 | Mpaka L Dissertation 2019.pdf | 3 MB | Adobe Acrobat PDF | View Details |