The reproductive biology and artificial breeding of ningu Labeo victorianus (Pisces: Cyprinidae)
- Authors: Rutaisire, Justus
- Date: 2004
- Subjects: Fishes -- Victoria, Lake , Cyprinidae -- Reproduction , Labeo -- Reproduction , Labeo
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5317 , http://hdl.handle.net/10962/d1005162
- Description: Lake Victoria, the largest tropical lake, has faced an unprecedented assault on its ecosystem through a variety of anthropomorphic causes that include the introduction of Nile perch Lates niloticus and over-fishing. As a result there have been species extinctions and declining fish population trends. This thesis explores options for reversing these declining population trends and the restoration of collapsed fisheries based on indigenous non-cichlid species. A candidate species was chosen - the cyprinid fish Labeo victorianus. This thesis studied various aspects of L. victorianus' reproductive biology in two geographically distant populations. These included general reproductive patterns in relation to proximate environmental conditions, gonadal development and recrudescence, sex development, characterisation of genetic and morphological diversity, and induced spawning. Fish from both populations were found to be potamodrometic, and highly fecund, iteroparous spawners. L. victorianus was also shown to be an undifferentiated gonochorist, where all individuals pass through an intersexual juvenile stage prior to differentiation, and maturation to either sex. Sexual maturity was attained at a significantly larger size within the Kagera River than that of Sio River fish – possibly as a response to genotypic and/or phenotypic differences. Spawning seemed to be synchronised with rainfall in Kagera River - a pattern that was not strictly adhered to with the Sio River. Thorough microscopic investigation of recrudescence patterns indicated there was uninterrupted spawning in fish from the Kagera River followed by Type I oocyte atresia. In contrast, there was a 90% spawning failure, as characterised by Type II oocyte atresia, within the Sio River population. Aspects of spermatogenesis and sperm ultrastructure using light- and ultramicrotomic methods are described. Although the populations had varying reproductive biology parameters and were morphologically distinct, they remained undifferentiated at the mitochondrial level. Both populations were characterised by low nucleotide diversity – a feature attributed to a bottleneck event. The option of captive breeding was explored by conducting induced spawning experiments. Success was only achieved with a decapeptide Gonadotropic Releasing Hormone ([D-Arg⁶, Pro⁹-NEt])-sGnRH) in combination with a water-soluble dopamine receptor antagonist metoclopramide. This thesis stresses the importance of a research-oriented approach in the conservation of Lake Victoria's indigenous fish resources. It was concluded that information needed for the development of management policies can be generated within a reasonably short time period, of approximately three years, with modest levels of funding support.
- Full Text:
- Date Issued: 2004
- Authors: Rutaisire, Justus
- Date: 2004
- Subjects: Fishes -- Victoria, Lake , Cyprinidae -- Reproduction , Labeo -- Reproduction , Labeo
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5317 , http://hdl.handle.net/10962/d1005162
- Description: Lake Victoria, the largest tropical lake, has faced an unprecedented assault on its ecosystem through a variety of anthropomorphic causes that include the introduction of Nile perch Lates niloticus and over-fishing. As a result there have been species extinctions and declining fish population trends. This thesis explores options for reversing these declining population trends and the restoration of collapsed fisheries based on indigenous non-cichlid species. A candidate species was chosen - the cyprinid fish Labeo victorianus. This thesis studied various aspects of L. victorianus' reproductive biology in two geographically distant populations. These included general reproductive patterns in relation to proximate environmental conditions, gonadal development and recrudescence, sex development, characterisation of genetic and morphological diversity, and induced spawning. Fish from both populations were found to be potamodrometic, and highly fecund, iteroparous spawners. L. victorianus was also shown to be an undifferentiated gonochorist, where all individuals pass through an intersexual juvenile stage prior to differentiation, and maturation to either sex. Sexual maturity was attained at a significantly larger size within the Kagera River than that of Sio River fish – possibly as a response to genotypic and/or phenotypic differences. Spawning seemed to be synchronised with rainfall in Kagera River - a pattern that was not strictly adhered to with the Sio River. Thorough microscopic investigation of recrudescence patterns indicated there was uninterrupted spawning in fish from the Kagera River followed by Type I oocyte atresia. In contrast, there was a 90% spawning failure, as characterised by Type II oocyte atresia, within the Sio River population. Aspects of spermatogenesis and sperm ultrastructure using light- and ultramicrotomic methods are described. Although the populations had varying reproductive biology parameters and were morphologically distinct, they remained undifferentiated at the mitochondrial level. Both populations were characterised by low nucleotide diversity – a feature attributed to a bottleneck event. The option of captive breeding was explored by conducting induced spawning experiments. Success was only achieved with a decapeptide Gonadotropic Releasing Hormone ([D-Arg⁶, Pro⁹-NEt])-sGnRH) in combination with a water-soluble dopamine receptor antagonist metoclopramide. This thesis stresses the importance of a research-oriented approach in the conservation of Lake Victoria's indigenous fish resources. It was concluded that information needed for the development of management policies can be generated within a reasonably short time period, of approximately three years, with modest levels of funding support.
- Full Text:
- Date Issued: 2004
The biology of commercially important fish species and a preliminary assessment of the fisheries potential of Katse Dam, Lesotho
- Authors: Nthimo, Mokitinyane Francis
- Date: 2000
- Subjects: Fishes -- Lesotho -- Katse dam , Fishes -- Physiology , Rainbow trout , Barbus aeneus , Labeo , Katse dam (Lesotho) , Fisheries -- Lesotho -- Katse dam , Fishery management -- Lesotho
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5238 , http://hdl.handle.net/10962/d1005081 , Fishes -- Lesotho -- Katse dam , Fishes -- Physiology , Rainbow trout , Barbus aeneus , Labeo , Katse dam (Lesotho) , Fisheries -- Lesotho -- Katse dam , Fishery management -- Lesotho
- Description: The construction of the Katse dam was completed in 1996. The dam started filling in 1995 and reached full capacity in early 1998. It forms part of Phase 1A of the Lesotho Highlands Water Project (LHWP). The main aim of the project is to provide revenue to Lesotho, by transferring water from the catchment of the Senqu (Orange) River in Lesotho to South Africa’s major industrial and population centres. During the implementation of the project, an estimated 130 people were displaced. However, the most serious impact has been the loss of the traditional way of life in the form of arable and grazing land as a consequence of inundation. One of the obligations of the project is to ensure that the standard of living of those affected by the project is not impaired. The Lesotho Highlands Development Authority identified a number of rural development projects, which included fisheries development. This study is an integral part of fisheries development in Lesotho. The aim of this study was to gain an understanding of the biology and the demographics of the fish species in the lake. This information would serve as the basis for the development of a management plan for the sustainable utilisation of the fisheries resources. To achieve this aim, the following specific objectives were addressed: 1. Investigation of the biology of the three principal species. 2. Description of the key population parameters (growth, mortality & recruitment). 3. Investigation of the distribution and relative abundance of the three species. The three principal species in the Katse dam are rainbow trout, Oncorhynchus mykiss, and the two cyprinids, Barbus aeneus and Labeo capensis. Sectioned otoliths were used to age O. mykiss while both otoliths and scales were used to age B. aeneus and L. capensis. Marginal zone analysis revealed that slow growth was experienced in winter for the three species. The maximum-recorded age for both B. aeneus and L. capensis was 12 years while O. mykiss reached 4 years. There was no significant difference in growth rates of the different sexes and growth was best described by the 3 parameter Von-Bertalanfy growth model as Lt = 603[1-e⁻°·¹⁵⁽t ⁺ °·°³⁾] for B. aeneus, Lt = 526[1-e⁻°·²¹⁽t ⁺ °·⁹⁾] for O. mykiss and Lt = 521[1-e⁻°·¹⁷⁽t ⁺ °·²¹⁾] for L.capensis. Male and female O. mykiss attained 50% sexual maturity (Lm₅₀) at 235 and 275mm FL, respectively. There was no difference in Lm₅₀ for male and female B. aeneus and L. capensis. B. aeneus reached sexual maturity at 285mm FL while L. capensis reached sexual maturity at 244mm. Both cyprinid species spawned in summer (November to January) while O. mykiss spawned in winter (May to August). The mean total mortality rate (Z) estimated from catch curves and Butterworth et al (1989) equation was 0.72 yr⁻¹ for B. aeneus, 0.62 yr⁻¹ for L. capensis and 1.32 yr⁻¹ for O. mykiss. The estimate of natural mortality was 0.41 yr⁻¹ for both B. aeneus and L. capensis and 0.81yr⁻¹ for O. mykiss.
- Full Text:
- Date Issued: 2000
- Authors: Nthimo, Mokitinyane Francis
- Date: 2000
- Subjects: Fishes -- Lesotho -- Katse dam , Fishes -- Physiology , Rainbow trout , Barbus aeneus , Labeo , Katse dam (Lesotho) , Fisheries -- Lesotho -- Katse dam , Fishery management -- Lesotho
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5238 , http://hdl.handle.net/10962/d1005081 , Fishes -- Lesotho -- Katse dam , Fishes -- Physiology , Rainbow trout , Barbus aeneus , Labeo , Katse dam (Lesotho) , Fisheries -- Lesotho -- Katse dam , Fishery management -- Lesotho
- Description: The construction of the Katse dam was completed in 1996. The dam started filling in 1995 and reached full capacity in early 1998. It forms part of Phase 1A of the Lesotho Highlands Water Project (LHWP). The main aim of the project is to provide revenue to Lesotho, by transferring water from the catchment of the Senqu (Orange) River in Lesotho to South Africa’s major industrial and population centres. During the implementation of the project, an estimated 130 people were displaced. However, the most serious impact has been the loss of the traditional way of life in the form of arable and grazing land as a consequence of inundation. One of the obligations of the project is to ensure that the standard of living of those affected by the project is not impaired. The Lesotho Highlands Development Authority identified a number of rural development projects, which included fisheries development. This study is an integral part of fisheries development in Lesotho. The aim of this study was to gain an understanding of the biology and the demographics of the fish species in the lake. This information would serve as the basis for the development of a management plan for the sustainable utilisation of the fisheries resources. To achieve this aim, the following specific objectives were addressed: 1. Investigation of the biology of the three principal species. 2. Description of the key population parameters (growth, mortality & recruitment). 3. Investigation of the distribution and relative abundance of the three species. The three principal species in the Katse dam are rainbow trout, Oncorhynchus mykiss, and the two cyprinids, Barbus aeneus and Labeo capensis. Sectioned otoliths were used to age O. mykiss while both otoliths and scales were used to age B. aeneus and L. capensis. Marginal zone analysis revealed that slow growth was experienced in winter for the three species. The maximum-recorded age for both B. aeneus and L. capensis was 12 years while O. mykiss reached 4 years. There was no significant difference in growth rates of the different sexes and growth was best described by the 3 parameter Von-Bertalanfy growth model as Lt = 603[1-e⁻°·¹⁵⁽t ⁺ °·°³⁾] for B. aeneus, Lt = 526[1-e⁻°·²¹⁽t ⁺ °·⁹⁾] for O. mykiss and Lt = 521[1-e⁻°·¹⁷⁽t ⁺ °·²¹⁾] for L.capensis. Male and female O. mykiss attained 50% sexual maturity (Lm₅₀) at 235 and 275mm FL, respectively. There was no difference in Lm₅₀ for male and female B. aeneus and L. capensis. B. aeneus reached sexual maturity at 285mm FL while L. capensis reached sexual maturity at 244mm. Both cyprinid species spawned in summer (November to January) while O. mykiss spawned in winter (May to August). The mean total mortality rate (Z) estimated from catch curves and Butterworth et al (1989) equation was 0.72 yr⁻¹ for B. aeneus, 0.62 yr⁻¹ for L. capensis and 1.32 yr⁻¹ for O. mykiss. The estimate of natural mortality was 0.41 yr⁻¹ for both B. aeneus and L. capensis and 0.81yr⁻¹ for O. mykiss.
- Full Text:
- Date Issued: 2000
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