- Title
- Molecular detection and drug susceptibility of Mycobacterium tuberculosis complex in raw milk from a major dairy farm in the Nkonkobe region, Eastern Cape Province, South Africa
- Creator
- Silaigwana, Blessing https://orcid.org/0000-0002-3324-1607
- Subject
- Mycobacterium tuberculosis
- Subject
- Drug resistance in microorganisms
- Subject
- Tuberculosis -- Pathogenesis
- Date
- 2012
- Type
- Master's theses
- Type
- text
- Identifier
- http://hdl.handle.net/10353/24239
- Identifier
- vital:62543
- Description
- Mycobacterium tuberculosis complex (MTBC) organisms are the causative agents of tuberculosis in humans as well as animals. The study aimed to use molecular techniques for detection and drug susceptibility testing of MTBC in raw milk from cattle at a dairy farm in the Nkonkobe region of South Africa. Two hundred samples (100mL each) were collected and processed using the modified Petroff’s method. DNA was isolated using the Zymo Research bacterial DNA kit and amplified using the Seeplex® MTB Nested ACE assay. Drug susceptibility testing was performed using the Genotype® MTBDRplus assay. MTBC DNA was detected in 11 (6percent) of the samples tested. Resistance to both rifampicin and isoniazid was detected in 90.9percent of the positive samples. The most frequent rpoB mutations detected were H526Y (90percent), H526D (80percent), S531L (60percent) and D516V (20percent). No mutation was detected in the katG gene. All isoniazid resistant samples harboured mutations in the inhA gene. The most frequent (100percent) mutation conferring low level isoniazid resistance was the T8A substitution. The inhA mutations C15T, A16G and T8C were equally represented with 60percent frequency. A high prevalence of multi-drug resistance was noted in the Nkonkobe region. Therefore, the results of this study have clinico-veterinary and epidemiological significance and calls for further studies and necessary actions to delineate the situation.
- Description
- Thesis (MSc) -- Faculty of Science and Agriculture, 2012
- Format
- computer
- Format
- online resource
- Format
- application/pdf
- Format
- 1 online resource (85 leaves)
- Format
- Publisher
- University of Fort Hare
- Publisher
- Faculty of Science and Agriculture
- Language
- English
- Rights
- University of Fort Hare
- Rights
- All Rights Reserved
- Rights
- Open Access
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Collections
UFH Department of Biochemistry & Microbiology
| Thumbnail | File | Description | Size | Format | |||
|---|---|---|---|---|---|---|---|
| View Details Download | SOURCE1 | M Sc Micro (May 2012) SALAIGWANE.pdf | 576 KB | Adobe Acrobat PDF | View Details Download |