- Title
- Evaluation of pharmacological properties and anti-neoplastic potentials of pelargonium iquinans and opuntia stricta extracts using acute leukemia cell lines
- Creator
- Izuegbuna, Ogochukwu Osita
- Subject
- Pelargoniums Medicinal plants
- Date
- 2018
- Type
- Thesis
- Type
- Masters
- Type
- M Sc
- Identifier
- http://hdl.handle.net/10353/10372
- Identifier
- vital:35442
- Description
- Leukaemia like other cancers is a public health issue, and coupled with the issue of drug resistance, toxicity and refractory disease novel treatments both as definitive and supportive care become necessary. The dissertation was designed to evaluate two medicinal plants (Opuntia stricta and Pelargonium inquinans) and their antineoplastic potentials. The phytochemicals analyses were done using standard methods with three different solvents (acetone, aqueous and ethanol). Vitamins analyses was also done using standard methods. The result revealed that vitamin A,C and E were present in the plants. Cell based anti-inflammatory activity were carried out using RAW264.7 macrophages cell, and COX-2 and NO expression as inflammatory markers. Cell based cytotoxicity and antineoplastic activity was investigated using RAW 264.7 macrophages cell, U937 and Jurkat cells. The results of the phytochemical analyses using GC/MS showed that phenols, flavonoids, flavonol, tannin, proanthocyanidin, saponin, alkaloids and phytate were present in these plants. In vitro antioxidant activity was carried out using nitric oxide (NO), alpha, alpha-diphenyl- β-picrylhydrazyl (DPPH), hydrogen peroxide (H2O2), phosphomolybdenum activity, and iron reducing power (FRAP) techniques. The results of the antioxidant activity showed both plants had antioxidant properties that can be attributed to the phytochemical and vitamins contents. The results showed that both plants had anti-inflammatory properties which can also be attributed to their phytochemicals. The GC-MS analysis of the essential oils of both plants showed the presence of monoterpenes, sesquiterpenes, fatty acids, and esters. The results showed that O. stricta extracts have mild cytotoxicity, while P. inquinans have strong cytotoxicity. The cell cycle analysis showed Pelargonium inquinans extracts induced G0/G1 phase arrest and cleaved caspase-3. This study has shown the antineoplastic potential of P. inquinans which may be attributed to some phytochemicals in it such as carophyllene, humulene, beta myrcene and beta pinene.
- Format
- 160 leaves
- Format
- Publisher
- University of Fort Hare
- Publisher
- Faculty of Science and Agriculture
- Language
- English
- Rights
- University of Fort Hare
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UFH Department of Biochemistry & Microbiology
| Thumbnail | File | Description | Size | Format | |||
|---|---|---|---|---|---|---|---|
| View Details Download | SOURCE1 | M Sc (Biochemistry) IZUEGBUNA - May 2018.pdf | 3 MB | Adobe Acrobat PDF | View Details Download |