Effects of BT Maize (MON810) crop and its residues on selected soil biological properties and N and P release in a sandy loam soil from Alice, Eastern Cape, South Africa
- Authors: Landzela, Besule
- Date: 2013
- Subjects: Bacillus (Bacteria) , Bacillus thuringiensis , Corn -- Planting , Biomass , Plant proteins , Enzymes , Soil fertility , Crop residue management
- Language: English
- Type: Thesis , Masters , MSc Agric (Crop Science)
- Identifier: vital:11870 , http://hdl.handle.net/10353/d1007542 , Bacillus (Bacteria) , Bacillus thuringiensis , Corn -- Planting , Biomass , Plant proteins , Enzymes , Soil fertility , Crop residue management
- Description: There are apprehensions that genetic modification of maize with Bacillus thuringiensis (Bt) may have negative effects on soil biodiversity, ecosystem processes and functions. This study aimed at determining the effect of Bt maize crop, Bt maize residues and its genetic modification on microbial biomass carbon (MBC), selected enzyme activities, vesicular arbuscular mycorrhizal (VAM) fungi and N and P release patterns. The study was conducted under field, glasshouse and laboratory conditions. In 2010/2011 season, four maize cultivars; DKC 61-25B (Bt), DKC 61-24 (non-Bt), PAN 6Q-321B (Bt) and PAN6777 (non-Bt) were planted. Determination of MBC, enzyme activities and fungal spore count was done at 42, 70, and 105 days after planting (DAP). A loam soil amended with Bt or non-Bt maize leaf residues from a study of 2009/2010 season was incubated to investigate effects of Bt maize residues on MBC and soil enzyme activities. Leaf residues of Bt and non-Bt maize cultivars (DKC 61-25B, DKC 61-24, PAN 6Q-321B and PAN6777) were used and soil without residues was used as a control. Samples were collected at 7, 28 and 56 days of incubation (DOI). An incubation study was also carried out in the laboratory to determine the effect of Bt maize residues (i.e. leaf, stem and root) and its genetic modification on N and P release patterns. Residues of DKC 61-25B, DKC 61-24, PAN 6Q-321B and PAN6777and soil without residues as a control were incubated in the laboratory. After destructive sampling at 0, 7, 14, 28, and 56 DOI, N in the form of NH4-N and NO3-N and P mineralisation were determined. Amendment of soil with residues enhanced MBC (p < 0.05) at all the sampling dates. For example MBC increased from 95 in the control to 146.3 mg/kg in the DKC 61-25B treatment at the end of the glasshouse trial. In the field DKC 61-25B had 9.1 mg/kg greater MBC than DKC 61-24, while PAN 6Q-321B had 23.9 mg/kg more MBC than PAN6777 at the end of the trial. However, no differences (p < 0.05) were observed in enzyme activities under field and glasshouse conditions except for dehydrogenase that had greater activity where DKC 61-25B and PAN 6777 were grown. There were no differences between the type of residues (Bt and non-Bt) on enzyme activities tested. However, differences were observed among the sampling dates. No effects of Bt maize crop on fungal spore count were observed. Similarly no differences were observed in leaf, stem and root tissues composition between Bt and non-Bt maize cultivars. Net N and P mineralisation from Bt maize cultivars did not differ from that of non-Bt maize cultivars. However, differences were observed among the cultivars. The results of this study suggested that Bt maize with Bt MON810 event can be grown in the central region of the Eastern Cape (EC), South Africa without affecting MBC, soil enzyme activities, VAM, and release of N and P nutrients from its residues.
- Full Text:
- Date Issued: 2013
- Authors: Landzela, Besule
- Date: 2013
- Subjects: Bacillus (Bacteria) , Bacillus thuringiensis , Corn -- Planting , Biomass , Plant proteins , Enzymes , Soil fertility , Crop residue management
- Language: English
- Type: Thesis , Masters , MSc Agric (Crop Science)
- Identifier: vital:11870 , http://hdl.handle.net/10353/d1007542 , Bacillus (Bacteria) , Bacillus thuringiensis , Corn -- Planting , Biomass , Plant proteins , Enzymes , Soil fertility , Crop residue management
- Description: There are apprehensions that genetic modification of maize with Bacillus thuringiensis (Bt) may have negative effects on soil biodiversity, ecosystem processes and functions. This study aimed at determining the effect of Bt maize crop, Bt maize residues and its genetic modification on microbial biomass carbon (MBC), selected enzyme activities, vesicular arbuscular mycorrhizal (VAM) fungi and N and P release patterns. The study was conducted under field, glasshouse and laboratory conditions. In 2010/2011 season, four maize cultivars; DKC 61-25B (Bt), DKC 61-24 (non-Bt), PAN 6Q-321B (Bt) and PAN6777 (non-Bt) were planted. Determination of MBC, enzyme activities and fungal spore count was done at 42, 70, and 105 days after planting (DAP). A loam soil amended with Bt or non-Bt maize leaf residues from a study of 2009/2010 season was incubated to investigate effects of Bt maize residues on MBC and soil enzyme activities. Leaf residues of Bt and non-Bt maize cultivars (DKC 61-25B, DKC 61-24, PAN 6Q-321B and PAN6777) were used and soil without residues was used as a control. Samples were collected at 7, 28 and 56 days of incubation (DOI). An incubation study was also carried out in the laboratory to determine the effect of Bt maize residues (i.e. leaf, stem and root) and its genetic modification on N and P release patterns. Residues of DKC 61-25B, DKC 61-24, PAN 6Q-321B and PAN6777and soil without residues as a control were incubated in the laboratory. After destructive sampling at 0, 7, 14, 28, and 56 DOI, N in the form of NH4-N and NO3-N and P mineralisation were determined. Amendment of soil with residues enhanced MBC (p < 0.05) at all the sampling dates. For example MBC increased from 95 in the control to 146.3 mg/kg in the DKC 61-25B treatment at the end of the glasshouse trial. In the field DKC 61-25B had 9.1 mg/kg greater MBC than DKC 61-24, while PAN 6Q-321B had 23.9 mg/kg more MBC than PAN6777 at the end of the trial. However, no differences (p < 0.05) were observed in enzyme activities under field and glasshouse conditions except for dehydrogenase that had greater activity where DKC 61-25B and PAN 6777 were grown. There were no differences between the type of residues (Bt and non-Bt) on enzyme activities tested. However, differences were observed among the sampling dates. No effects of Bt maize crop on fungal spore count were observed. Similarly no differences were observed in leaf, stem and root tissues composition between Bt and non-Bt maize cultivars. Net N and P mineralisation from Bt maize cultivars did not differ from that of non-Bt maize cultivars. However, differences were observed among the cultivars. The results of this study suggested that Bt maize with Bt MON810 event can be grown in the central region of the Eastern Cape (EC), South Africa without affecting MBC, soil enzyme activities, VAM, and release of N and P nutrients from its residues.
- Full Text:
- Date Issued: 2013
Soil fertility enhancement through appropriate fertilizer management on winter cover crops in a conservation agriculture system
- Authors: Dube, Ernest
- Date: 2012
- Subjects: Fertilizers , Soil fertility , Crops -- Nutrition , Soil productivity , Range management , Grazing -- Management , Forage plants , Humus
- Language: English
- Type: Thesis , Doctoral , PhD (Crop Science)
- Identifier: vital:11239 , http://hdl.handle.net/10353/d1001044 , Fertilizers , Soil fertility , Crops -- Nutrition , Soil productivity , Range management , Grazing -- Management , Forage plants , Humus
- Description: A study was carried out to determine the effects of oat (Avena sativa) and grazing vetch (Vicia darsycapa) winter cover crops and fertilizer application on SOM, phosphorus (P) pools, nutrient availability, nutrient uptake, maize yield and seedbanks of problematic weeds in an irrigated maize-based conservation agriculture (CA) system. A separate experiment was carried out to investigate the effects of the winter cover crops on nitrogen (N) management, N use efficiency and profitability. After four years of continuous rotation, the winter cover crops significantly (p<0.05) increased particulate SOM and hot water soluble carbon in the 0 – 5 and 5 – 20 cm soil depths. When fertilized, oat was better able to support SOM sequestration in water stable aggregates at 0 – 20 cm whilst grazing vetch was more effective at 20 – 50 cm. Where no fertilizer was invested, there were significant (p<0.01) reductions in biomass input and SOM on oat-maize and weedy fallow-maize rotations whereas vetch-maize rotations did not respond, both at 0 – 5 and 5 – 20 cm. Targeting fertilizer to the winter cover crop required less fertilizer, and yet gave a similar SOM response as targeting the fertilizer to the maize crop. In addition to increasing SOM in the surface soil (0 – 5 cm), the winter cover crops significantly (p<0.05) increased labile pools of P, including microbial P. The cover crops also significantly (p<0.05) increased maize P concentration during early growth, extractable soil P, Cu, Mn, and Zn but had no effect on Ca and K. Grazing vetch increased soil mineral N but reduced extractable soil Mg. Without fertilizer, there were sharp declines in maize grain yield on oat and weedy fallow rotations over the four year period, but less so, on the grazing vetch. Grazing vetch increased maize growth, grain yield response to N fertilizer, nitrogen use efficiency (NUE) and profitability for fertilizer rates below 180 kg N ha-1. Oat effects however on maize yield and NUE were generally similar to weedy fallow. Based on the partial factor productivity of N, the highest efficiencies in utilization of fertilizer N for maize yield improvement under grazing vetch and oat are obtained at 60 kg N ha-1 and would decline thereafter with any increases in fertilizer application rate. Grazing vetch gave N fertilizer replacement values of up to 120 kg N ha-1 as well as the highest marginal rates of return to increasing N fertilizer rate. The cover crops were more effective than the weedy fallow in reducing seedbank density of Digitaria sanguinalis, Eleusine indica, Amaranthus retroflexus and Datura stramonium at 0 – 5 cm soil depth, causing weed seed reductions of 30 - 70%. The winter cover crops however, selectively allowed emergence of the narrow leafed weeds; Cyperus esculentus and Digitaria sanguinalis in the maize crop. The findings of this study suggested that grazing vetch is suited for SOM improvement in low fertilizer input systems and that fertilizer is better invested on winter cover crops as opposed to maize crops. Oat, on the other hand, when fertilized, would be ideal for C sequestration in water stable aggregates of the surface soil. Grazing vetch is ideal for resource poor farmers who cannot afford mineral fertilizers as it gives grain yield improvement and high fertilizer replacement value. Grazing vetch can produce enough maize yield response to pay its way in the maize-based systems and oat may not require additional N than that applied to the weedy fallow. Phosphorus and Zn are some of the major limiting essential plant nutrients on South African soils and the winter cover crops could make a contribution. The cover crops also hasten depletion of some problematic weeds from seedbanks, leading to reduced weed pressure during maize growth.
- Full Text:
- Date Issued: 2012
- Authors: Dube, Ernest
- Date: 2012
- Subjects: Fertilizers , Soil fertility , Crops -- Nutrition , Soil productivity , Range management , Grazing -- Management , Forage plants , Humus
- Language: English
- Type: Thesis , Doctoral , PhD (Crop Science)
- Identifier: vital:11239 , http://hdl.handle.net/10353/d1001044 , Fertilizers , Soil fertility , Crops -- Nutrition , Soil productivity , Range management , Grazing -- Management , Forage plants , Humus
- Description: A study was carried out to determine the effects of oat (Avena sativa) and grazing vetch (Vicia darsycapa) winter cover crops and fertilizer application on SOM, phosphorus (P) pools, nutrient availability, nutrient uptake, maize yield and seedbanks of problematic weeds in an irrigated maize-based conservation agriculture (CA) system. A separate experiment was carried out to investigate the effects of the winter cover crops on nitrogen (N) management, N use efficiency and profitability. After four years of continuous rotation, the winter cover crops significantly (p<0.05) increased particulate SOM and hot water soluble carbon in the 0 – 5 and 5 – 20 cm soil depths. When fertilized, oat was better able to support SOM sequestration in water stable aggregates at 0 – 20 cm whilst grazing vetch was more effective at 20 – 50 cm. Where no fertilizer was invested, there were significant (p<0.01) reductions in biomass input and SOM on oat-maize and weedy fallow-maize rotations whereas vetch-maize rotations did not respond, both at 0 – 5 and 5 – 20 cm. Targeting fertilizer to the winter cover crop required less fertilizer, and yet gave a similar SOM response as targeting the fertilizer to the maize crop. In addition to increasing SOM in the surface soil (0 – 5 cm), the winter cover crops significantly (p<0.05) increased labile pools of P, including microbial P. The cover crops also significantly (p<0.05) increased maize P concentration during early growth, extractable soil P, Cu, Mn, and Zn but had no effect on Ca and K. Grazing vetch increased soil mineral N but reduced extractable soil Mg. Without fertilizer, there were sharp declines in maize grain yield on oat and weedy fallow rotations over the four year period, but less so, on the grazing vetch. Grazing vetch increased maize growth, grain yield response to N fertilizer, nitrogen use efficiency (NUE) and profitability for fertilizer rates below 180 kg N ha-1. Oat effects however on maize yield and NUE were generally similar to weedy fallow. Based on the partial factor productivity of N, the highest efficiencies in utilization of fertilizer N for maize yield improvement under grazing vetch and oat are obtained at 60 kg N ha-1 and would decline thereafter with any increases in fertilizer application rate. Grazing vetch gave N fertilizer replacement values of up to 120 kg N ha-1 as well as the highest marginal rates of return to increasing N fertilizer rate. The cover crops were more effective than the weedy fallow in reducing seedbank density of Digitaria sanguinalis, Eleusine indica, Amaranthus retroflexus and Datura stramonium at 0 – 5 cm soil depth, causing weed seed reductions of 30 - 70%. The winter cover crops however, selectively allowed emergence of the narrow leafed weeds; Cyperus esculentus and Digitaria sanguinalis in the maize crop. The findings of this study suggested that grazing vetch is suited for SOM improvement in low fertilizer input systems and that fertilizer is better invested on winter cover crops as opposed to maize crops. Oat, on the other hand, when fertilized, would be ideal for C sequestration in water stable aggregates of the surface soil. Grazing vetch is ideal for resource poor farmers who cannot afford mineral fertilizers as it gives grain yield improvement and high fertilizer replacement value. Grazing vetch can produce enough maize yield response to pay its way in the maize-based systems and oat may not require additional N than that applied to the weedy fallow. Phosphorus and Zn are some of the major limiting essential plant nutrients on South African soils and the winter cover crops could make a contribution. The cover crops also hasten depletion of some problematic weeds from seedbanks, leading to reduced weed pressure during maize growth.
- Full Text:
- Date Issued: 2012
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