Lifting the veil on the "Untouchable": a study of Muslims living with HIV in Durban, South Africa
- Authors: Shaik, Shabnam
- Date: 2023-10-13
- Subjects: AIDS (Disease) Religious aspects Islam , HIV infections Religious aspects Islam , AIDS (Disease) (Islamic law) South Africa , Muslims South Africa Durban , Traditional medicine South Africa , Integrative medicine
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/432518 , vital:72877 , DOI 10.21504/10962/432519
- Description: HIV and AIDS has been prominently studied, by both biomedical and social scientists, since the 1980s. Despite the extensive research that has emerged globally, Muslims have largely been absent in much of the discourse on HIV and AIDS. While the disease is said to be under control in many parts of the world, hidden populations pose a risk to increase in prevalence. Muslims living with HIV are one such hidden population and are only recently gaining representation in studies on HIV and AIDS. Quantitative studies reflect a sharp increase in HIV prevalence in countries with significant Muslim populations, however, more in-depth qualitative studies are needed to garner an understanding of the nature of the disease among Muslims. Muslims have a long history in South Africa, beginning with indentured slavery in Natal (now KwaZulu-Natal) in 1860. There are no specific HIV and AIDS prevention campaigns directed at Muslims in Durban, KwaZulu-Natal, South Africa. Muslims in Durban do not regard HIV is a significant concern for them due to religious doctrine, which they believe if followed will keep them safe from infection. My findings suggest that there is cause for concern over the spread of HIV and AIDS amongst Muslims in Durban. The hidden nature of the disease in the Muslim community has created challenges to curbing the spread of the disease due to the concealment of its presence and the marginalisation of Muslims living with HIV and AIDS. This study conceptualises the HIV and AIDS lived experience amongst Muslims in Durban, South Africa, and uncovers the social and cultural context of the disease. The qualitative study used life histories with ten Muslims living with HIV and semi-structured and unstructured interviews with ten caregivers, five health care professionals and two religious leaders to gain a detailed understanding of the lived experiences of Muslims in Durban. Through the lenses of Purity and Danger, Stigma and Spoilt Identity, and Social Death, this study found that religion [Islam] and its strong moral code influences understandings and perceptions of HIV and AIDS which, in turn affects diagnosis, treatment, care, social identity, and the social well-being of Muslims living with HIV who face stigma, discrimination, shame, and ostracisation from their own community. This study highlights the gaps in the literature on Muslims living with HIV and by providing insight into the lives of participants, the study draws attention to the plight of Muslims living with HIV and AIDS (MLWHIV) and encourages more qualitative studies to be conducted so that a multifaceted understanding of Muslims living with HIV can be created. , Thesis (PhD) -- Faculty of Humanities, Anthropology, 2023
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- Date Issued: 2023-10-13
Pharmaco-chemical investigation of Erythrina caffra: extracts, isolated compounds and their biological activities
- Authors: Nogqala, Simnikiwe
- Date: 2023-03-29
- Subjects: Coast coral tree , Traditional medicine South Africa , Antibacterial agents , Antineoplastic agents , Organic compounds
- Language: English
- Type: Academic theses , Master's theses , text
- Identifier: http://hdl.handle.net/10962/422459 , vital:71944
- Description: In this study, secondary metabolites isolated from Erythrina caffra, a medicinal plant indigenous to South Africa, were investigated. E. caffra is well-known for its healing properties and it is traditionally used for treating bacterial infections like tuberculosis (TB), abscesses, tooth aches and ear infections. Its extracts have also been used to treat cancer. Though many studies have been done on this plant, most of them tended to focus solely on the isolated compounds. In the present study however, extracts, fractions and isolated compounds from E. caffra were evaluated for their anticancer, anti-oxidant, anti-enzymatic, antibacterial and cytotoxicity. The methanol crude extract (B1) from the stem bark of E. caffra was used to extract alkaloidic fractions (B2 and B3) using ethyl acetate and n-butanol respectively, a third fraction (B4) was also extracted using ethyl acetate this fraction was called a neutral fraction. The neutral fraction (B4) was fractionated and through a sequence of column chromatography three active secondary metabolites were isolated. The isolated compounds included Lupeol (1), stigmasterol (2) and 5,7-Dihydroxy-4'-methoxy-3',5'-diprenylflavanone (3). These isolated compounds were characterized and identified using spectroscopic techniques including IR, NMR and high-resolution Mass Spectrometry. Using the cell line HCC-70, isolated from a primary ductal carcinoma, in vitro anticancer assays were carried out on the crude extract from the bark, fractions, isolated compounds and an unseparated mixture of two compounds. These samples were also evaluated for their anti-oxidant, anti-enzymatic, antibacterial and cytotoxicity activities. The crude extract inhibited the cell viability by over 30% and had no effect on the HeLa cells at concentrations of 20μM. Abyssinone V’ 4-methyl-ether (3) and the mixture of stigmasterol (2) and an unidentified compound exhibited potent anticancer activity against the HCC-70 cell line with IC50 of 18.05μM and 9.04μM respectively. Antibacterial assays were also carried out on the crude extracts, fractions and concoctions made from the fractions with the best activity combined with the ones that performed poorly. The concoctions were prepared as two separate series (S and N series). The crude extract inhibited more than 80% of the Staphylococcus aureus cells at a concentration of 20μM with only minimal damage to the HeLa cells. In the concoctions however, the N series managed to inhibit over 96% of the S. aureus while exhibiting no cytotoxicity towards HeLa cells. The extract and its fractions also showed good anti-oxidant activities. Molecular docking of these compounds was done on the Human estrogen receptor (PDB ID:3ERT) and Abyssinone V’ 4-methyl-ether (3) showed the best docking score of -6.6 Kcal/mol, for the simulation against Epidermal growth factor receptor (PDB ID: 1M17) Stigmasterol (2) showed the best docking score of -3.8 Kcal/mol. In silico docking on 3ERT and 1M17 were done to test the binding affinity of the isolated compounds to the proteins which are well known to be overexpressed in some types of cancer. Flavonoids isolated from Erythrina species have been reported to possess good antiplasmodial activity. However, due to the minute amounts isolated in the present study in-vitro assays could not be carried out. Nevertheless, in-silico assays were conducted on the most prominent protozoal parasite which causes malaria in the majority of African countries. In-silico simulations were done against Plasmodium falciparum protein (PDB ID: 7KJH), of the tested compounds Abyssinone V’ 4-methyl-ether (3) was found possess the best docking score of -4.4 Kcal/mol. The molecular docking of 7KJH was done to assess the inhibitory potential of the isolated compounds on protozoal parasites. Pharmacokinetic properties of the isolated compounds were also assessed in silico to assist in evaluating the drug likeness of these compounds. The compounds showed a percent human oral absorption of 100% except for Abyssinone V’ 4-methyl-ether (3), which showed 93.83%, this indicates a remarkable oral bioavailability. Stigamsterol (2) exhibited a Caco-2 cell permeability (QPPCaco) greater than 500 which indicates outstanding results for good intestinal absorption. The compounds also displayed a blood-brain partition co-efficient (QPlogBB) ranging from -1.433 to 0.128 suggesting they will have less potential to cross the blood-brain barrier, thus reducing any CNS related toxicity. Molecular networking of the crude extracts and the fractions was done through GNPS which allowed the identification of known compounds including one isolated in the present study, Abyssinone V’ 4-methyl-ether (3). Possible derivatives that have not been isolated from this plant before were also putatively identified. , Thesis (MSc) -- Faculty of Science, Chemistry, 2023
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- Date Issued: 2023-03-29