A green approach for the synthesis of symmetrical and unsymmetrical 1,2,4,5-tetraoxanes as anti-protozoal agents
- Authors: Cossa, Teresa Manuel
- Date: 2021-10-29
- Subjects: Uncatalogued
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/192786 , vital:45264
- Description: Thesis (PhD) -- Faculty of Science, Chemistry, 2021
- Full Text:
- Date Issued: 2021-10-29
- Authors: Cossa, Teresa Manuel
- Date: 2021-10-29
- Subjects: Uncatalogued
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/192786 , vital:45264
- Description: Thesis (PhD) -- Faculty of Science, Chemistry, 2021
- Full Text:
- Date Issued: 2021-10-29
An investigation into yeast-baculovirus synergism for the improved control of Thaumatotibia leucotreta, an economically important pest of citrus
- Authors: Van der Merwe, Marcél
- Date: 2021-10-29
- Subjects: Baculoviruses , Cryptophlebia leucotreta , Yeast , Natural pesticides , Citrus Diseases and pests , Biological pest control agents , Pests Integrated control , Thaumatotibia leucotreta
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/191236 , vital:45073
- Description: A mutualistic association between Cydia pomonella and yeasts belonging to the genus Metschnikowia has previously been demonstrated. Larval feeding galleries inoculated with M. andauensis, reduced larval mortality and enhanced larval development. Additionally, adult C. pomonella female oviposition preference was also shown to be influenced by the volatiles produced by M. andauensis. This mutualistic relationship was manipulated for biological control purposes, by combining M. pulcherrima with the baculovirus Cydia pomonella granulovirus. The combination of M. pulcherrima with brown cane sugar and CpGV in laboratory assays and field trials resulted in a significant increase in larval mortality. A similar observation was made when M. pulcherrima was substituted for Saccharomyces cerevisiae. This indicates that yeasts harbour the potential for use in biological control, especially when combined with other well-established biocontrol methods. Thaumatotibia leucotreta is a phytophagous insect endemic to southern Africa. It is highly significant to the South African citrus industry due to its classification as a phytosanitary pest by most international markets. An integrated pest management programme has been implemented to control T. leucotreta. The baculovirus Cryptophlebia leucotreta granulovirus forms one component of this programme and is highly effective. In this study, we proposed to determine which yeast species occur naturally in the gut of T. leucotreta larvae and to examine whether any of the isolated yeast species, when combined with the CrleGV-SA, enhance its effectiveness. Firstly, Navel oranges infested with T. leucotreta larvae were collected from geographically distinct citrus-producing regions across South Africa. This led to the isolation and identification of six yeast species from the gut of T. leucotreta larvae via PCR amplification and sequencing of the internal transcribed spacer region and D1/D2 domain of the large subunit. Six yeast species were identified, viz. Meyerozyma guilliermondii, Hanseniaspora uvarum, Clavispora lusitaniae, Kluyveromyces marxianus, Pichia kudriavzevii and Pichia kluyveri. Additionally, Saccharomyces cerevisiae was included as a control in all trials due to its commercial availability and use in the artificial diet used to rear T. leucotreta. Secondly, larval development and attraction assays were conducted with the isolated yeast species. Thaumatotibia leucotreta larvae that fed on Navel oranges inoculated with M. guilliermondii, P. kluyveri, H. uvarum, and S. cerevisiae had accelerated developmental periods and reduced mortality rates. Additionally, it was demonstrated that T. leucotreta neonates were attracted to YPD broth cultures inoculated with P. kluyveri, H. uvarum, P. kudriavzevii and K. marxianus for feeding. Thirdly, oviposition preference assays were conducted with adult T. leucotreta females to determine whether the isolated yeast species influence their egg-laying in two-choice and multiple-choice tests. Navel oranges were inoculated with a specific yeast isolate, and mated adult females were left to oviposit. Meyerozyma guilliermondii, P. kudriavzevii and H. uvarum were shown to influence adult T. leucotreta female oviposition preference in two-choice tests. However, multiple-choice tests using the aforementioned yeast species did not mimic these results. Lastly, a series of detached fruit bioassays were performed to determine the optimal yeast:virus ratio, test all isolated yeast species in combination with CrleGV-SA and to further enhance yeast/virus formulation through the addition of an adjuvant and surfactant. CrleGV-SA was applied at a lethal concentration that would kill 50 % of T. leucotreta larvae. The optimal yeast concentration to use alongside CrleGV-SA was determined. Pichia kluyveri, P. kudriavzevii, K. marxianus and S. cerevisiae in combination with CrleGV-SA increased larval mortality compared to CrleGV-SA alone. The inclusion of molasses and BREAK-THRU® S 240 to P. kudriavzevii and S. cerevisiae plus CrleGV-SA formulations greatly enhanced their efficacy. Additionally, semi-field trials were initiated using P. kudriavzevii and S. cerevisiae, with promising preliminary results being obtained, although more replicates need to be performed. The experiments performed in this study provide a platform for further research into the application of a yeast/virus combination as a novel control and monitoring option for T. leucotreta in the field. , Thesis (PhD) -- Faculty of Science, Biochemistry and Microbiology, 2021
- Full Text:
- Date Issued: 2021-10-29
- Authors: Van der Merwe, Marcél
- Date: 2021-10-29
- Subjects: Baculoviruses , Cryptophlebia leucotreta , Yeast , Natural pesticides , Citrus Diseases and pests , Biological pest control agents , Pests Integrated control , Thaumatotibia leucotreta
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/191236 , vital:45073
- Description: A mutualistic association between Cydia pomonella and yeasts belonging to the genus Metschnikowia has previously been demonstrated. Larval feeding galleries inoculated with M. andauensis, reduced larval mortality and enhanced larval development. Additionally, adult C. pomonella female oviposition preference was also shown to be influenced by the volatiles produced by M. andauensis. This mutualistic relationship was manipulated for biological control purposes, by combining M. pulcherrima with the baculovirus Cydia pomonella granulovirus. The combination of M. pulcherrima with brown cane sugar and CpGV in laboratory assays and field trials resulted in a significant increase in larval mortality. A similar observation was made when M. pulcherrima was substituted for Saccharomyces cerevisiae. This indicates that yeasts harbour the potential for use in biological control, especially when combined with other well-established biocontrol methods. Thaumatotibia leucotreta is a phytophagous insect endemic to southern Africa. It is highly significant to the South African citrus industry due to its classification as a phytosanitary pest by most international markets. An integrated pest management programme has been implemented to control T. leucotreta. The baculovirus Cryptophlebia leucotreta granulovirus forms one component of this programme and is highly effective. In this study, we proposed to determine which yeast species occur naturally in the gut of T. leucotreta larvae and to examine whether any of the isolated yeast species, when combined with the CrleGV-SA, enhance its effectiveness. Firstly, Navel oranges infested with T. leucotreta larvae were collected from geographically distinct citrus-producing regions across South Africa. This led to the isolation and identification of six yeast species from the gut of T. leucotreta larvae via PCR amplification and sequencing of the internal transcribed spacer region and D1/D2 domain of the large subunit. Six yeast species were identified, viz. Meyerozyma guilliermondii, Hanseniaspora uvarum, Clavispora lusitaniae, Kluyveromyces marxianus, Pichia kudriavzevii and Pichia kluyveri. Additionally, Saccharomyces cerevisiae was included as a control in all trials due to its commercial availability and use in the artificial diet used to rear T. leucotreta. Secondly, larval development and attraction assays were conducted with the isolated yeast species. Thaumatotibia leucotreta larvae that fed on Navel oranges inoculated with M. guilliermondii, P. kluyveri, H. uvarum, and S. cerevisiae had accelerated developmental periods and reduced mortality rates. Additionally, it was demonstrated that T. leucotreta neonates were attracted to YPD broth cultures inoculated with P. kluyveri, H. uvarum, P. kudriavzevii and K. marxianus for feeding. Thirdly, oviposition preference assays were conducted with adult T. leucotreta females to determine whether the isolated yeast species influence their egg-laying in two-choice and multiple-choice tests. Navel oranges were inoculated with a specific yeast isolate, and mated adult females were left to oviposit. Meyerozyma guilliermondii, P. kudriavzevii and H. uvarum were shown to influence adult T. leucotreta female oviposition preference in two-choice tests. However, multiple-choice tests using the aforementioned yeast species did not mimic these results. Lastly, a series of detached fruit bioassays were performed to determine the optimal yeast:virus ratio, test all isolated yeast species in combination with CrleGV-SA and to further enhance yeast/virus formulation through the addition of an adjuvant and surfactant. CrleGV-SA was applied at a lethal concentration that would kill 50 % of T. leucotreta larvae. The optimal yeast concentration to use alongside CrleGV-SA was determined. Pichia kluyveri, P. kudriavzevii, K. marxianus and S. cerevisiae in combination with CrleGV-SA increased larval mortality compared to CrleGV-SA alone. The inclusion of molasses and BREAK-THRU® S 240 to P. kudriavzevii and S. cerevisiae plus CrleGV-SA formulations greatly enhanced their efficacy. Additionally, semi-field trials were initiated using P. kudriavzevii and S. cerevisiae, with promising preliminary results being obtained, although more replicates need to be performed. The experiments performed in this study provide a platform for further research into the application of a yeast/virus combination as a novel control and monitoring option for T. leucotreta in the field. , Thesis (PhD) -- Faculty of Science, Biochemistry and Microbiology, 2021
- Full Text:
- Date Issued: 2021-10-29
Microalgal-bacterial flocs and extracellular polymeric substances for optimum function of integrated algal pond systems
- Authors: Jimoh, Taobat Adekilekun
- Date: 2021-10-29
- Subjects: Flocculation , Extracellular polymeric substances , Water Purification , Sewage Purification Anaerobic treatment , Integrated algae pond systems (IAPS) , Microalgal-bacterial flocs
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/191214 , vital:45071 , 10.21504/10962/191214
- Description: Despite the dire state of sanitation infrastructures, water scarcity, and the dwindling reserve of natural resources due to ever-increasing population growth, implementation of a suitable technology that can provide a solution to all these issues continues to be ignored. The integrated algal pond system (IAPS) is a wastewater treatment technology that combines the processes of anaerobic digestion and photosynthetic oxygenation to achieve wastewater treatment and facilitate the recovery of treated water and resources in the form of biogas and microalgal-bacterial biomass. The natural process of bioflocculation through microalgal-bacterial mutualism and production of extracellular polymeric substances (EPS) in high rate algal oxidation ponds (HRAOPs) of an IAPS increases efficiency of wastewater treatment and potentially enhances harvestability and biomass recovery, which could contribute significantly to the successful establishment of a biorefinery. Using a 500 PE pilot-scale IAPS supplied domestic sewage coupled with laboratory experiments, this study investigated the importance and function of in situ EPS production and MaB-floc formation in HRAOP. A metagenomic study revealed the biological components of the biomass or mixed liquor suspended solids (MLSS) produced in HRAOP and showed that the suspended biomass is composed largely of eukaryotes that were dominated by the colonial microalgae Pseudopediastrum sp. and Desmodesmus sp., and a diverse range of prokaryotes including bacteria and cyanobacteria. Dominance, within the bacterial population, by a sulphur-oxidizing bacterium, Thiothrix which comprised up to 80% of the prokaryotes, coincided with a period of poor flocculation and was therefore rationalized to have contributed to bulking and poor biomass settleability. Otherwise, good flocs were formed in the MLSS with settleability up to 95% and, within 1 h. The formation of MaB-flocs appeared to be dependent on EPS concentration of the mixed liquor due to the observed positive correlation between soluble EPS (S-EPS), biomass concentration, and settleability. The contribution and role of MLSS components towards the formation and sustenance of MaB-flocs were further demonstrated in laboratory experiments using pure strains of microalgae, cyanobacteria, and bacteria. Results showed that pure cultures of dominant microalgae in MLSS, Pseudopediastrum sp. and Desmodesmus sp. achieved a rapid 92 and 75% settleability within 3 h. A self-flocculating filamentous cyanobacterium, Leptolyngbya strain ECCN 20BG was isolated, characterized, and shown to achieve 99% settleability within 5 min by forming large tightly aggregated flocs. In further experiments, this strain was found to improve the settleability of MLSS by an average of 20%. Bacterial strains identified as Bacillus strain ECCN 40b, Bacillus strain ECCN 41b, Planococcus strain ECCN 45b, and Exiguobacterium strain ECCN 46b were also observed to produce sticky EPS-like materials in pure cultures that could also contribute to the aggregation of cells in a mixed environment. Given these results, various factors and/or mechanisms that might enhance microbial aggregation and biomass recovery from HRAOP MLSS were identified in this study and include; (1) dominance by larger colonial microalgae prevents disintegration of MaB-flocs and enhances recovery of biomass from MLSS by gravity sedimentation, (2) presence of filamentous cyanobacteria species that can self-flocculate to form an interwoven network of filaments may play an important role in the structural stability and settleability of MaB-flocs in MLSS, and (3) production of EPS to form the matrix or scaffold whereon all microbial components aggregate to develop a microenvironment. Indeed, all forms of EPS, except for that produced by Bacillus strain ECCN 41b, showed bioflocculating property and were able to serve as flocculants for the recovery of Chlorella, an alga known for its poor settleability. A combination of biochemical analyses and FTIR spectroscopy revealed the importance of carbohydrate enrichment of these biopolymers. Carbohydrate concentration in all forms of EPS was between 12 and 41% suggesting that production of these compounds by microbes within the MLSS contributed to MaB-floc formation. EPS extracted from bulk MLSS and EPS produced by Bacillus strains possessed some surface-active properties that were comparable to Triton X-100, indicating potential application in bioremediation and recovery of oil from contaminated soil and water. In particular, EPS generated from Bacillus strain ECCN 41b displayed relatively distinct properties including the quantity produced (> 500 mg/L), increased viscosity, inability to flocculate microalgal cells, a rhamnolipid content of 32%, and a higher surface-activity. Based on these results, Bacillus strain ECCN 41b was rationalized to produce anionic EPS with potential application in metal or oil recovery. In addition to EPS production, the bacteria Planococcus strain ECCN 45b and Exiguobacterium strain ECCN 46b appeared pigmented. Based on partial characterization using UV/Vis spectrophotometry, thin-layer chromatography, FTIR, and NMR, the pigments produced by these two strains appeared to be identical and were tentatively identified as ketocarotenoids. This study successfully demonstrated the importance of EPS production and formation of MaB-flocs in the MLSS from HRAOP of an IAPS treating domestic sewage. It is evident that increased settleability of the biomass does contribute to the reported efficiency of wastewater treatment by IAPS and would reduce both total suspended solids (TSS) and chemical oxygen demand (COD). In addition, demonstration that this biomass contains products of value such as carotenoids and EPS with potential for commercial use strengthens the idea of using IAPS as a platform technology for innovation of the wastewater treatment process to a biorefinery. , Thesis (PhD) -- Faculty of Science, Institute for Environmental Biotechnology, 2021
- Full Text:
- Date Issued: 2021-10-29
- Authors: Jimoh, Taobat Adekilekun
- Date: 2021-10-29
- Subjects: Flocculation , Extracellular polymeric substances , Water Purification , Sewage Purification Anaerobic treatment , Integrated algae pond systems (IAPS) , Microalgal-bacterial flocs
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/191214 , vital:45071 , 10.21504/10962/191214
- Description: Despite the dire state of sanitation infrastructures, water scarcity, and the dwindling reserve of natural resources due to ever-increasing population growth, implementation of a suitable technology that can provide a solution to all these issues continues to be ignored. The integrated algal pond system (IAPS) is a wastewater treatment technology that combines the processes of anaerobic digestion and photosynthetic oxygenation to achieve wastewater treatment and facilitate the recovery of treated water and resources in the form of biogas and microalgal-bacterial biomass. The natural process of bioflocculation through microalgal-bacterial mutualism and production of extracellular polymeric substances (EPS) in high rate algal oxidation ponds (HRAOPs) of an IAPS increases efficiency of wastewater treatment and potentially enhances harvestability and biomass recovery, which could contribute significantly to the successful establishment of a biorefinery. Using a 500 PE pilot-scale IAPS supplied domestic sewage coupled with laboratory experiments, this study investigated the importance and function of in situ EPS production and MaB-floc formation in HRAOP. A metagenomic study revealed the biological components of the biomass or mixed liquor suspended solids (MLSS) produced in HRAOP and showed that the suspended biomass is composed largely of eukaryotes that were dominated by the colonial microalgae Pseudopediastrum sp. and Desmodesmus sp., and a diverse range of prokaryotes including bacteria and cyanobacteria. Dominance, within the bacterial population, by a sulphur-oxidizing bacterium, Thiothrix which comprised up to 80% of the prokaryotes, coincided with a period of poor flocculation and was therefore rationalized to have contributed to bulking and poor biomass settleability. Otherwise, good flocs were formed in the MLSS with settleability up to 95% and, within 1 h. The formation of MaB-flocs appeared to be dependent on EPS concentration of the mixed liquor due to the observed positive correlation between soluble EPS (S-EPS), biomass concentration, and settleability. The contribution and role of MLSS components towards the formation and sustenance of MaB-flocs were further demonstrated in laboratory experiments using pure strains of microalgae, cyanobacteria, and bacteria. Results showed that pure cultures of dominant microalgae in MLSS, Pseudopediastrum sp. and Desmodesmus sp. achieved a rapid 92 and 75% settleability within 3 h. A self-flocculating filamentous cyanobacterium, Leptolyngbya strain ECCN 20BG was isolated, characterized, and shown to achieve 99% settleability within 5 min by forming large tightly aggregated flocs. In further experiments, this strain was found to improve the settleability of MLSS by an average of 20%. Bacterial strains identified as Bacillus strain ECCN 40b, Bacillus strain ECCN 41b, Planococcus strain ECCN 45b, and Exiguobacterium strain ECCN 46b were also observed to produce sticky EPS-like materials in pure cultures that could also contribute to the aggregation of cells in a mixed environment. Given these results, various factors and/or mechanisms that might enhance microbial aggregation and biomass recovery from HRAOP MLSS were identified in this study and include; (1) dominance by larger colonial microalgae prevents disintegration of MaB-flocs and enhances recovery of biomass from MLSS by gravity sedimentation, (2) presence of filamentous cyanobacteria species that can self-flocculate to form an interwoven network of filaments may play an important role in the structural stability and settleability of MaB-flocs in MLSS, and (3) production of EPS to form the matrix or scaffold whereon all microbial components aggregate to develop a microenvironment. Indeed, all forms of EPS, except for that produced by Bacillus strain ECCN 41b, showed bioflocculating property and were able to serve as flocculants for the recovery of Chlorella, an alga known for its poor settleability. A combination of biochemical analyses and FTIR spectroscopy revealed the importance of carbohydrate enrichment of these biopolymers. Carbohydrate concentration in all forms of EPS was between 12 and 41% suggesting that production of these compounds by microbes within the MLSS contributed to MaB-floc formation. EPS extracted from bulk MLSS and EPS produced by Bacillus strains possessed some surface-active properties that were comparable to Triton X-100, indicating potential application in bioremediation and recovery of oil from contaminated soil and water. In particular, EPS generated from Bacillus strain ECCN 41b displayed relatively distinct properties including the quantity produced (> 500 mg/L), increased viscosity, inability to flocculate microalgal cells, a rhamnolipid content of 32%, and a higher surface-activity. Based on these results, Bacillus strain ECCN 41b was rationalized to produce anionic EPS with potential application in metal or oil recovery. In addition to EPS production, the bacteria Planococcus strain ECCN 45b and Exiguobacterium strain ECCN 46b appeared pigmented. Based on partial characterization using UV/Vis spectrophotometry, thin-layer chromatography, FTIR, and NMR, the pigments produced by these two strains appeared to be identical and were tentatively identified as ketocarotenoids. This study successfully demonstrated the importance of EPS production and formation of MaB-flocs in the MLSS from HRAOP of an IAPS treating domestic sewage. It is evident that increased settleability of the biomass does contribute to the reported efficiency of wastewater treatment by IAPS and would reduce both total suspended solids (TSS) and chemical oxygen demand (COD). In addition, demonstration that this biomass contains products of value such as carotenoids and EPS with potential for commercial use strengthens the idea of using IAPS as a platform technology for innovation of the wastewater treatment process to a biorefinery. , Thesis (PhD) -- Faculty of Science, Institute for Environmental Biotechnology, 2021
- Full Text:
- Date Issued: 2021-10-29
Plant-fungal mutualism as a strategy for the bioremediation of hydrocarbon polluted soils
- Authors: Keshinro, Olajide Muritala
- Date: 2021-10-29
- Subjects: Mutualism (Biology) , Plant-fungus relationships , Bioremediation , Mucilage , Plant exudates , Extracellular polymeric substances , Laccase , Peroxidase , Phytoremediation , Ligninolytic enzymes
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/190918 , vital:45041 , 10.21504/10962/190918
- Description: Inasmuch as coal remains the linchpin for the generation of electricity and liquid petroleum products in South Africa, hydrocarbon waste and coal discard will continue to pose a threat to the environment. Therefore, the onus is on the associated industries to develop and implement efficient and sustainable strategies to mitigate the negative impacts of energy generating activities on the environment. Most conventional efforts in this regard, although successful for soil repair and the initiation of vegetation, have been deemed unsustainable. In an effort to find a sustainable remediation strategy a novel technology termed “FungCoal” was conceptualized and patented as a strategy for the rehabilitation of open cast coal mines, carbonaceous-rich spoils and coal wastes. This biotechnology, which exploits plant-fungal mutualism to achieve effective biodegradation of coal on discard dumps and the breakdown of the carbonaceous component in spoils, promotes revegetation to facilitate rehabilitation of mining-disturbed land. However, one limiting factor of the FungCoal bioprocess is that it requires oxidized weathered coal, a highly complex and variable resource for use as a co-substrate, for growth and proliferation of the coal degrading microorganisms. To fully exploit the potential of plant-fungal mutualism and its interaction for use in the remediation of coal contaminated soils, this study investigated the proposed relationship between plant roots, root exudate and the coal degrading fungus “Aspergillus sp.” (previously Neosartorya fischeri) strain 84 in more detail, in an effort to gain further insight into the mechanisms underpinning plant-fungal mutualism as a strategy for re-vegetation of coal discard dumps and the rehabilitation of hydrocarbon-contaminated soil using the FungCoal approach. A pot-on-beaker (PoB) method was developed for the easy cultivation and collection of extracellular polymeric substance (EPS)-containing exudates from Zea mays L. (maize) and Abelmuschus esculentus (okra). Characterisation of the EPS material from these exudates was carried out using a combination of physicochemical and biochemical methods. The results from analysis of phenolics and indoles showed that exudates contain some form of indoles and phenolic compounds, although in little proportions, which may fulfil a signalling function, responsible for attracting soil microorganisms into the rhizosphere. Spectroscopic analysis of the exudates using FT-IR revealed vibrations corresponding to functional groups of alkanes, alkenes, alkynes, and carboxylic acids. These compounds likely provide an easily accessible source of carbon to soil microorganisms and are also a better alternative to the poly-aromatics which are an inherent component locked-up in the supposed recalcitrant coal material. The results from biochemical analyses also revealed the presence of carbohydrate, proteins, lipids, and low amounts of α-amino-nitrogen in the EPS of maize and okra. These components of EPS are all essential for the stimulation of enzymatic activities in soil microorganisms and, which may in turn aid biodegradation. The action of the root EPS from maize was further tested on three coal-degrading fungal isolates identified as Aspergillus strain ECCN 84, Aspergillus strain ECCN 225 and Penicillium strain ECCN 243 for manganese peroxidase (MnP) and laccase (LAC) activities. The results revealed that the Aspergillus species, strains ECCN 84 and ECCN 225, showed with or without EPS, observable black halos surrounding each of the colonies after 7d incubation indicative of positive MnP activity, while no activity was observed for the Penicillium sp. strain ECCN 243. Analysis for LAC revealed little or no activity in any of the coal degrading fungi following addition of pulverized coal to the growth medium. Interestingly, the addition of EPS-containing exudate to the coal-containing medium resulted in increased LAC activity for all fungal isolates. This finding affirmed the positive contribution of EPS to extracellular LAC activity, purported as an important enzyme in the coal biodegradation process. Finally, the impact of plant-derived exudate on the colonisation and biodegradation of coal was investigated in situ using rhizoboxes, to simulate a coal environment, and was carried out for 16 weeks. Microscopic examination of coal samples after termination of the experiment showed fungal proliferation and attachment to coal particles. All of the rhizoboxes that contained plants had higher medium pH and EC, and the concentration of phenolics, indoles and humic acids was greater than that of control treatments. These observations indicated better rhizosphere colonisation, substrate biodegradation and humification. Therefore, root exudate appears to play a significant role in coordination of soil microorganisms within the rhizosphere and likely serves both as a scaffold for rhizospheric interactions by providing microorganisms with accessible carbon and as a likely ‘trigger’ for induction of coal-degrading enzymes such as fungal LAC for mobilisation of recalcitrant carbon. This study has shown that EPS exuded from roots of Zea mays together with coal degrading fungus Aspergillus strain ECCN 84 can alkalinise the coal substrate and facilitate introduction of oxygen, possibly as a result of increased laccase activity, and increase availability of nutrients (as indicated by higher EC) in a coal-polluted rhizosphere, to provide plants and their associated mycorrhizae and presumably other beneficial microorganisms a more mesic environment for sustained phytoremediation with enhanced rehabilitation potential. In conclusion, this study confirms the positive role of root exudate in mediating a mutualistic rehabilitation strategy involving plants and fungi such as the FungCoal bioprocess. , Thesis (PhD) -- Faculty of Science, Institute for Environmental Biotechnology, 2021
- Full Text:
- Date Issued: 2021-10-29
- Authors: Keshinro, Olajide Muritala
- Date: 2021-10-29
- Subjects: Mutualism (Biology) , Plant-fungus relationships , Bioremediation , Mucilage , Plant exudates , Extracellular polymeric substances , Laccase , Peroxidase , Phytoremediation , Ligninolytic enzymes
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/190918 , vital:45041 , 10.21504/10962/190918
- Description: Inasmuch as coal remains the linchpin for the generation of electricity and liquid petroleum products in South Africa, hydrocarbon waste and coal discard will continue to pose a threat to the environment. Therefore, the onus is on the associated industries to develop and implement efficient and sustainable strategies to mitigate the negative impacts of energy generating activities on the environment. Most conventional efforts in this regard, although successful for soil repair and the initiation of vegetation, have been deemed unsustainable. In an effort to find a sustainable remediation strategy a novel technology termed “FungCoal” was conceptualized and patented as a strategy for the rehabilitation of open cast coal mines, carbonaceous-rich spoils and coal wastes. This biotechnology, which exploits plant-fungal mutualism to achieve effective biodegradation of coal on discard dumps and the breakdown of the carbonaceous component in spoils, promotes revegetation to facilitate rehabilitation of mining-disturbed land. However, one limiting factor of the FungCoal bioprocess is that it requires oxidized weathered coal, a highly complex and variable resource for use as a co-substrate, for growth and proliferation of the coal degrading microorganisms. To fully exploit the potential of plant-fungal mutualism and its interaction for use in the remediation of coal contaminated soils, this study investigated the proposed relationship between plant roots, root exudate and the coal degrading fungus “Aspergillus sp.” (previously Neosartorya fischeri) strain 84 in more detail, in an effort to gain further insight into the mechanisms underpinning plant-fungal mutualism as a strategy for re-vegetation of coal discard dumps and the rehabilitation of hydrocarbon-contaminated soil using the FungCoal approach. A pot-on-beaker (PoB) method was developed for the easy cultivation and collection of extracellular polymeric substance (EPS)-containing exudates from Zea mays L. (maize) and Abelmuschus esculentus (okra). Characterisation of the EPS material from these exudates was carried out using a combination of physicochemical and biochemical methods. The results from analysis of phenolics and indoles showed that exudates contain some form of indoles and phenolic compounds, although in little proportions, which may fulfil a signalling function, responsible for attracting soil microorganisms into the rhizosphere. Spectroscopic analysis of the exudates using FT-IR revealed vibrations corresponding to functional groups of alkanes, alkenes, alkynes, and carboxylic acids. These compounds likely provide an easily accessible source of carbon to soil microorganisms and are also a better alternative to the poly-aromatics which are an inherent component locked-up in the supposed recalcitrant coal material. The results from biochemical analyses also revealed the presence of carbohydrate, proteins, lipids, and low amounts of α-amino-nitrogen in the EPS of maize and okra. These components of EPS are all essential for the stimulation of enzymatic activities in soil microorganisms and, which may in turn aid biodegradation. The action of the root EPS from maize was further tested on three coal-degrading fungal isolates identified as Aspergillus strain ECCN 84, Aspergillus strain ECCN 225 and Penicillium strain ECCN 243 for manganese peroxidase (MnP) and laccase (LAC) activities. The results revealed that the Aspergillus species, strains ECCN 84 and ECCN 225, showed with or without EPS, observable black halos surrounding each of the colonies after 7d incubation indicative of positive MnP activity, while no activity was observed for the Penicillium sp. strain ECCN 243. Analysis for LAC revealed little or no activity in any of the coal degrading fungi following addition of pulverized coal to the growth medium. Interestingly, the addition of EPS-containing exudate to the coal-containing medium resulted in increased LAC activity for all fungal isolates. This finding affirmed the positive contribution of EPS to extracellular LAC activity, purported as an important enzyme in the coal biodegradation process. Finally, the impact of plant-derived exudate on the colonisation and biodegradation of coal was investigated in situ using rhizoboxes, to simulate a coal environment, and was carried out for 16 weeks. Microscopic examination of coal samples after termination of the experiment showed fungal proliferation and attachment to coal particles. All of the rhizoboxes that contained plants had higher medium pH and EC, and the concentration of phenolics, indoles and humic acids was greater than that of control treatments. These observations indicated better rhizosphere colonisation, substrate biodegradation and humification. Therefore, root exudate appears to play a significant role in coordination of soil microorganisms within the rhizosphere and likely serves both as a scaffold for rhizospheric interactions by providing microorganisms with accessible carbon and as a likely ‘trigger’ for induction of coal-degrading enzymes such as fungal LAC for mobilisation of recalcitrant carbon. This study has shown that EPS exuded from roots of Zea mays together with coal degrading fungus Aspergillus strain ECCN 84 can alkalinise the coal substrate and facilitate introduction of oxygen, possibly as a result of increased laccase activity, and increase availability of nutrients (as indicated by higher EC) in a coal-polluted rhizosphere, to provide plants and their associated mycorrhizae and presumably other beneficial microorganisms a more mesic environment for sustained phytoremediation with enhanced rehabilitation potential. In conclusion, this study confirms the positive role of root exudate in mediating a mutualistic rehabilitation strategy involving plants and fungi such as the FungCoal bioprocess. , Thesis (PhD) -- Faculty of Science, Institute for Environmental Biotechnology, 2021
- Full Text:
- Date Issued: 2021-10-29
Systematics of the Afrotropical Chalcididae (Hymenoptera: Chalcidoidea)
- Authors: Faure, Sariana
- Date: 2021-10-29
- Subjects: Uncatalogued
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/192808 , vital:45266
- Description: Thesis (PhD) -- Faculty of Science, Zoology and Entomology, 2021
- Full Text:
- Date Issued: 2021-10-29
- Authors: Faure, Sariana
- Date: 2021-10-29
- Subjects: Uncatalogued
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/192808 , vital:45266
- Description: Thesis (PhD) -- Faculty of Science, Zoology and Entomology, 2021
- Full Text:
- Date Issued: 2021-10-29
The biological control of Egeria densa Planch. (Hydrocharitaceae) in South Africa
- Authors: Smith, Rosali
- Date: 2021-10-29
- Subjects: Egeria (Plant genus) Biological control South Africa , Hydrocharitaceae Biological control South Africa , Aquatic weeds Biological control South Africa , Leafminers South Africa , Plant invasions South Africa , Resilience (Ecology) South Africa , Freshwater ecology South Africa , Hydrellia South Africa , Submerged macrophyte
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/191102 , vital:45060 , 10.21504/10962/191102
- Description: Over the last thirty years, biological control, the use of host-specific natural enemies, has been a huge asset in the management exotic aquatic macrophytes such as Pistia stratiotes L. (Araceae), Pontederia crassipes Mart. (Solms) (Pontederiaceae), Azolla filiculoides Lam. (Azollaceae), Salvinia molesta D.S. Mitch (Salviniaceae) and Myriophyllum aquaticum (Vell.) Verdc. (Haloragaceae), also known as the “Big Bad Five” in South Africa. Despite these successes, freshwater ecosystems in South Africa have been harder to restore to an invasive macrophyte-free space, due to chronic disturbances such eutrophication, propagule dispersal and hydrological alterations. In the Anthropocene, where human activities have profound effects on their environment, these disturbances weakens ecological resilience and drive aquatic plant invasions. Due to long periods of invasions and the presence of a new suite of exotic aquatic plant propagules, native vegetation recolonization has been slow or even absent. Instead, the release of resources, such as sunlight, nutrient and space through aquatic weed management acts as a catalyst for secondary biological invasion. New invasive aquatic weeds include submerged and rooted emergent growth types, with Egeria densa Planch. (Hydrocharitaceae) the most widely distributed submerged aquatic weed in South Africa. It can quickly form dense monoculture stands that have ecological, economic and social impacts. Because of its ability to regenerate from plant fragments with double nodes, mechanical control is inappropriate. Additionally, mechanical and chemical control not only affects E. densa but have significant non-target effects. In response to its rapid spread over the last 20 years, especially following floating invasive aquatic management, a biological control programme was initiated, and in 2018, the leaf-mining fly, Hydrellia egeriae Rodrigues (Diptera: Ephydridae) was released. This was the first release of a biological control agent against E. densa in the world, and the first agent released against a submerged aquatic weed in South Africa. This thesis comprises the subsequent step of a biological control program when permission for the release of an agent have been obtained. A brief history of macrophyte invasions in South Africa’s unique freshwater systems are given in the literature review. Contributing factors to secondary invasions within the context of ecological resilience are introduced. An argument for the benefit of biological control as nuisance control is given, especially because E. densa and its natural enemy, H. egeriae is the focus species of this thesis. The main goal after permission for the release of an agent have been obtained, is to establish and build-up field populations. Research questions in this thesis aimed to investigate factors that contribute to or negate this goal. Through laboratory and field experiments we investigated the thermal physiology of the agent, and its climatic suitability to its novel range; different release strategies on field establishment and biotic resistance through the acquisition of novel parasitoids. Considering the longevity of this biological control program, we investigated the effects of elevated CO2 on the interaction between E. densa and H. egeriae through open top chamber experiments. Laboratory thermal physiology results showed that the agent is able to survive, develop and proliferate at all E. densa sites throughout the year. This is confirmed with the establishment of the agent at two release sites, the Nahoon River in the Eastern Cape Province and the Midmar Dam in KwaZulu-Natal. Post-release surveys showed that H. egeriae requires augmentative releases to sustain field populations. Without augmentative releases, H. egeriae herbivory levels were almost negligent. However, a contributing factor to low field-populations was parasitism. The biological control agent acquired three parasitoids, which have previously been described from Hydrellia lagarosiphon Deeming (Diptera: Ephydridae), a specific herbivore to Lagarosiphon major (Ridl.) Moss (Hydrocharitaceae). These results provide information on the immediate establishment and effectiveness of the H. egeriae. Results from the elevated CO2 study suggest that E. densa will become less nutritious through a shift in leaf C/N ratio, when ambient 800ppm is bubbled into experimental growth chambers. Hydrellia egeriae feeding was affected by ambient CO2 levels and plant nutrient availability. The set levels of ambient CO2 levels used in this experiment produced dissolved inorganic carbon levels that were lower than dissolved inorganic carbon levels in E. densa invaded sites. This suggests that, submerged aquatic plant-insect interactions may be harder to predict from only laboratory experiments. Further investigations are necessary to establish system-specific characteristics i.e. dissolved inorganic carbon and target plant nutritional quality. The biological control of E. densa in South Africa is still in its infancy. This study presents results from post-release surveys up until two years after the agent was released. From this study, Hydrellia egeriae exhibits the potential to be an effective biological control agent, but release strategies should be adapted to sustain field populations and to limit field parasitism effects. Continued post-release surveys will provide a more comprehensive idea of the seasonal fluctuations of field-populations and parasitism. Surveys at multiple sites will provide information on potential site specific characteristics that contribute to or negate biological effort. Considering the high nutrient status of South African freshwater systems, a more holistic approach to E. densa management is necessary. This will require the strengthening of ecological resilience to prevent systems from shifting into an alternate invasive stable state. In addition, aquatic weed management needs to be addressed by a resilient social network, which ultimately calls for the strengthening of socio-ecological resilience. , Thesis (PhD) -- Faculty of Science, Zoology and Entomology, 2021
- Full Text:
- Date Issued: 2021-10-29
- Authors: Smith, Rosali
- Date: 2021-10-29
- Subjects: Egeria (Plant genus) Biological control South Africa , Hydrocharitaceae Biological control South Africa , Aquatic weeds Biological control South Africa , Leafminers South Africa , Plant invasions South Africa , Resilience (Ecology) South Africa , Freshwater ecology South Africa , Hydrellia South Africa , Submerged macrophyte
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/191102 , vital:45060 , 10.21504/10962/191102
- Description: Over the last thirty years, biological control, the use of host-specific natural enemies, has been a huge asset in the management exotic aquatic macrophytes such as Pistia stratiotes L. (Araceae), Pontederia crassipes Mart. (Solms) (Pontederiaceae), Azolla filiculoides Lam. (Azollaceae), Salvinia molesta D.S. Mitch (Salviniaceae) and Myriophyllum aquaticum (Vell.) Verdc. (Haloragaceae), also known as the “Big Bad Five” in South Africa. Despite these successes, freshwater ecosystems in South Africa have been harder to restore to an invasive macrophyte-free space, due to chronic disturbances such eutrophication, propagule dispersal and hydrological alterations. In the Anthropocene, where human activities have profound effects on their environment, these disturbances weakens ecological resilience and drive aquatic plant invasions. Due to long periods of invasions and the presence of a new suite of exotic aquatic plant propagules, native vegetation recolonization has been slow or even absent. Instead, the release of resources, such as sunlight, nutrient and space through aquatic weed management acts as a catalyst for secondary biological invasion. New invasive aquatic weeds include submerged and rooted emergent growth types, with Egeria densa Planch. (Hydrocharitaceae) the most widely distributed submerged aquatic weed in South Africa. It can quickly form dense monoculture stands that have ecological, economic and social impacts. Because of its ability to regenerate from plant fragments with double nodes, mechanical control is inappropriate. Additionally, mechanical and chemical control not only affects E. densa but have significant non-target effects. In response to its rapid spread over the last 20 years, especially following floating invasive aquatic management, a biological control programme was initiated, and in 2018, the leaf-mining fly, Hydrellia egeriae Rodrigues (Diptera: Ephydridae) was released. This was the first release of a biological control agent against E. densa in the world, and the first agent released against a submerged aquatic weed in South Africa. This thesis comprises the subsequent step of a biological control program when permission for the release of an agent have been obtained. A brief history of macrophyte invasions in South Africa’s unique freshwater systems are given in the literature review. Contributing factors to secondary invasions within the context of ecological resilience are introduced. An argument for the benefit of biological control as nuisance control is given, especially because E. densa and its natural enemy, H. egeriae is the focus species of this thesis. The main goal after permission for the release of an agent have been obtained, is to establish and build-up field populations. Research questions in this thesis aimed to investigate factors that contribute to or negate this goal. Through laboratory and field experiments we investigated the thermal physiology of the agent, and its climatic suitability to its novel range; different release strategies on field establishment and biotic resistance through the acquisition of novel parasitoids. Considering the longevity of this biological control program, we investigated the effects of elevated CO2 on the interaction between E. densa and H. egeriae through open top chamber experiments. Laboratory thermal physiology results showed that the agent is able to survive, develop and proliferate at all E. densa sites throughout the year. This is confirmed with the establishment of the agent at two release sites, the Nahoon River in the Eastern Cape Province and the Midmar Dam in KwaZulu-Natal. Post-release surveys showed that H. egeriae requires augmentative releases to sustain field populations. Without augmentative releases, H. egeriae herbivory levels were almost negligent. However, a contributing factor to low field-populations was parasitism. The biological control agent acquired three parasitoids, which have previously been described from Hydrellia lagarosiphon Deeming (Diptera: Ephydridae), a specific herbivore to Lagarosiphon major (Ridl.) Moss (Hydrocharitaceae). These results provide information on the immediate establishment and effectiveness of the H. egeriae. Results from the elevated CO2 study suggest that E. densa will become less nutritious through a shift in leaf C/N ratio, when ambient 800ppm is bubbled into experimental growth chambers. Hydrellia egeriae feeding was affected by ambient CO2 levels and plant nutrient availability. The set levels of ambient CO2 levels used in this experiment produced dissolved inorganic carbon levels that were lower than dissolved inorganic carbon levels in E. densa invaded sites. This suggests that, submerged aquatic plant-insect interactions may be harder to predict from only laboratory experiments. Further investigations are necessary to establish system-specific characteristics i.e. dissolved inorganic carbon and target plant nutritional quality. The biological control of E. densa in South Africa is still in its infancy. This study presents results from post-release surveys up until two years after the agent was released. From this study, Hydrellia egeriae exhibits the potential to be an effective biological control agent, but release strategies should be adapted to sustain field populations and to limit field parasitism effects. Continued post-release surveys will provide a more comprehensive idea of the seasonal fluctuations of field-populations and parasitism. Surveys at multiple sites will provide information on potential site specific characteristics that contribute to or negate biological effort. Considering the high nutrient status of South African freshwater systems, a more holistic approach to E. densa management is necessary. This will require the strengthening of ecological resilience to prevent systems from shifting into an alternate invasive stable state. In addition, aquatic weed management needs to be addressed by a resilient social network, which ultimately calls for the strengthening of socio-ecological resilience. , Thesis (PhD) -- Faculty of Science, Zoology and Entomology, 2021
- Full Text:
- Date Issued: 2021-10-29
An appraisal of the East London Industrial Development Zone’s role in local innovation, entrepreneurship, and industrial development
- Authors: Masiwa, George Bonginkosi
- Date: 2021-02
- Subjects: Industrialization , Economic development , South Africa -- Economic conditions
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/21213 , vital:47406
- Description: This study focuses on the East London Industrial Development Zone (ELIDZ) in South Africa. The ELIDZ strategy came as a response to the need for a robust catalyst for sustainable regional economic development, industrial diversification and to create meaningful employment opportunities in the country. It was supposed to provide customised solutions for various industries including automotive, agro-processing and aqua-culture. However, an Industrial Development Zone should promote innovati on and entrepreneurship to stimulate economic growth and global competitiveness. Economic stagnation, joblessness and inequality continue to be a reality for the previously marginalised groups after 16 years of the ELIDZ despite significant investments in the automotive sector, the growth of companies that supply Mercedes Benz with parts and the establishment and growth of innovative start-up companies at the ELIDZ Science and Technology Park (STP). Massive joblessness, poverty and economic stagnation had followed the collapse of the old industrial parks. The ELIDZ strategy of industrial development came as a response to the demise of the old parks and was meant to become the main vehicle for job creation, local innovation and global competitiveness. This study therefore investigated the extent to which innovation and entrepreneurship have fared in driving sustainable local economic development and how they have impacted jobs at the ELIDZ and within the East London community. The study was qualitative in nature and employed the non-probability sampling technique. In-depth semi-structured interviews were conducted with key informants that included Industrialists, Business Executives that were tenants at the ELIDZ, the Buffalo City Municipal Metro and community members with knowledge of and association with the ELIDZ. This was augmented by desktop and observational data. It was found that innovation and entrepreneurship was still a fairly new phenomenon at the ELIDZ and their flagships had the vision but were not yet driving sustainable local economic development to a large extent. The model lacks adequate diversification as most of the innovation projects are in the renewable energy and information communication spaces. Very few have made it to mass production with downstream industry employment creation for plumbers, artisans and marketers involved in the supply, distribution, instalment and maintenance of the Hotspot and Thin-film solar panels. New industrialists like YEKANI Technologies and Manufacturing and KGI Holdings, already employ some people and have the vision to employ thousands more of young people. The main innovation and entrepreneurship projects of the ELIDZ have had some impact at the grassroots level even though it is still limited. Too few jobs have been created compared to the old industrial parks model. The ELIDZ is therefore playing an important (albeit limited) role as a local employment creator, even though it has not revitalised the Eastern Cape manufacturing landscape. Due to its highly technology driven and specialised skills nature, the ELIDZ model is benefiting the few and therefore has not yet been an effective instrument to resolve the disturbing levels of inequality, poverty and unemployment, that underlie racial, class and gender dynamics in East London. The study concluded that innovation and entrepreneurship projects at the ELIDZ were contributing towards driving sustainable local economic development to some degree. However, due to its narrow industrial focus and highly technical and specialised skills driven business model, it excludes most historically disadvantaged communities and the local academic institutions need to do more to produce the skills relevant to support local innovation and local industrial development in a time when economies rely more on intellectual capabilities rather than natural resources. , Thesis (PhD) -- Faculty of Social Sciences and Humanities, 2021
- Full Text:
- Date Issued: 2021-02
- Authors: Masiwa, George Bonginkosi
- Date: 2021-02
- Subjects: Industrialization , Economic development , South Africa -- Economic conditions
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/21213 , vital:47406
- Description: This study focuses on the East London Industrial Development Zone (ELIDZ) in South Africa. The ELIDZ strategy came as a response to the need for a robust catalyst for sustainable regional economic development, industrial diversification and to create meaningful employment opportunities in the country. It was supposed to provide customised solutions for various industries including automotive, agro-processing and aqua-culture. However, an Industrial Development Zone should promote innovati on and entrepreneurship to stimulate economic growth and global competitiveness. Economic stagnation, joblessness and inequality continue to be a reality for the previously marginalised groups after 16 years of the ELIDZ despite significant investments in the automotive sector, the growth of companies that supply Mercedes Benz with parts and the establishment and growth of innovative start-up companies at the ELIDZ Science and Technology Park (STP). Massive joblessness, poverty and economic stagnation had followed the collapse of the old industrial parks. The ELIDZ strategy of industrial development came as a response to the demise of the old parks and was meant to become the main vehicle for job creation, local innovation and global competitiveness. This study therefore investigated the extent to which innovation and entrepreneurship have fared in driving sustainable local economic development and how they have impacted jobs at the ELIDZ and within the East London community. The study was qualitative in nature and employed the non-probability sampling technique. In-depth semi-structured interviews were conducted with key informants that included Industrialists, Business Executives that were tenants at the ELIDZ, the Buffalo City Municipal Metro and community members with knowledge of and association with the ELIDZ. This was augmented by desktop and observational data. It was found that innovation and entrepreneurship was still a fairly new phenomenon at the ELIDZ and their flagships had the vision but were not yet driving sustainable local economic development to a large extent. The model lacks adequate diversification as most of the innovation projects are in the renewable energy and information communication spaces. Very few have made it to mass production with downstream industry employment creation for plumbers, artisans and marketers involved in the supply, distribution, instalment and maintenance of the Hotspot and Thin-film solar panels. New industrialists like YEKANI Technologies and Manufacturing and KGI Holdings, already employ some people and have the vision to employ thousands more of young people. The main innovation and entrepreneurship projects of the ELIDZ have had some impact at the grassroots level even though it is still limited. Too few jobs have been created compared to the old industrial parks model. The ELIDZ is therefore playing an important (albeit limited) role as a local employment creator, even though it has not revitalised the Eastern Cape manufacturing landscape. Due to its highly technology driven and specialised skills nature, the ELIDZ model is benefiting the few and therefore has not yet been an effective instrument to resolve the disturbing levels of inequality, poverty and unemployment, that underlie racial, class and gender dynamics in East London. The study concluded that innovation and entrepreneurship projects at the ELIDZ were contributing towards driving sustainable local economic development to some degree. However, due to its narrow industrial focus and highly technical and specialised skills driven business model, it excludes most historically disadvantaged communities and the local academic institutions need to do more to produce the skills relevant to support local innovation and local industrial development in a time when economies rely more on intellectual capabilities rather than natural resources. , Thesis (PhD) -- Faculty of Social Sciences and Humanities, 2021
- Full Text:
- Date Issued: 2021-02
An exploratory analysis of the challenges faced by police in combating mob justice: a case study of Gqeberha
- Loqani Anelisa https://orcid.org/0000-0001-9944-5947
- Authors: Loqani Anelisa https://orcid.org/0000-0001-9944-5947
- Date: 2021-02
- Subjects: Vigilance committees , Criminal justice, Administration of
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/22000 , vital:51928
- Description: Mob justice is a major issue that will continue to occur as long as criminal activities exist. In South Africa, mob justice has become a major challenge for the government, particularly the South African Police Service as well as the society at large. The South African Police Service (SAPS) has partnered with all relevant role-players in the fight against mob justice and other community problems which threaten the safety of inhabitants. However, concerted efforts have also been made by the government to assist the SAPS to address the occurrence and re-occurrence of mob justice by introducing strategies such as visible policing but the problem seems to persist. The study, thus, analyzed the challenges faced by the police that lead to their ineffectiveness in eliminating mob justice. This thesis draws from two theories, namely Structural Violence theory pioneered by Galtung (1969) and Just World Theory (JWT) by Adelstein (1999) to explore the challenges faced by the police in Gqeberha. This study was conducted in the Eastern Cape Province, in Gqeberha within four townships, namely Kwadwesi, Kwazakhele, Motherwell, and New Brighton. The findings gathered by utilizing Focus Group Interviews (FGIs) and semi-structured interviews with a total number of forty-eight (48) respondents comprising of sixteen (16) SAPS members, eight (08) Community Policing Forum (CPF) representatives, four (04) community leaders and twenty (20) community members. This is a qualitative research project in which participants were chosen using purposive sampling. Participants were chosen based on their knowledge and experiences relating to the phenomena being explored. The aim was to gain an in-depth understanding of the challenges encountered by the police in combating mob justice to discover some techniques to improve and strengthen the police service to make communities safer. To analyse the data thematic analysis was utilized. The results of the study indicate that an increase in mob justice in Gqeberha has been reported to be a result of the high rate of crime. However, the fact that people know they will not get arrested for participating in mob justice is one of the concerns that have led to an escalation in mob justice. The study demonstrates that in most incidents the community members have once witnessed in their respective areas, the victims were killed while few survived and in all those incidents only a few arrests were made and in those few arrests, no convictions were made. Based on this issue of arrests, results indicate that the SAPS members are confronting some difficulties in identifying and arresting the perpetrators of mob justice. The reluctance of community members to report mob justice cases and to provide information was found to be the main challenge faced by the police in combating mob justice in Motherwell Cluster precincts. These challenges have been reported to negatively affect police work performance. Further analysis revealed that CPF, community members and community leaders as part of stakeholders who are working together with SAPS are also facing some challenges in addressing this issue of mob justice. The results also indicate that mob justice does not only harm members of the community who are subjected to these occurrences, but also the police, the victims and the perpetrators themselves. Evidence-based techniques to help eradicate mob justice incidents were developed in response to this problem of mob justice. The participants suggested that the police must be provided with adequate manpower, more community patrols and vehicles. It is believed that if the police could act in time and arrest the suspected criminals, a large number of people would be rescued from being killed in mob justice. The respondents also indicated that the police must build good relations with the communities by being visible and transparent in communities. Furthermore, the SAPS participants alluded that when people are sentenced, a community engagement needs to be conducted before those people are released outside as parolees. Other respondents mentioned the issue of education, proposing that the Criminal Justice System (CJS) should frequently conduct awareness campaigns, imbizos and public meetings to teach and inform people about its operations. , Thesis (PhD) -- Faculty of Social Sciences and Humanities, 2021
- Full Text:
- Date Issued: 2021-02
- Authors: Loqani Anelisa https://orcid.org/0000-0001-9944-5947
- Date: 2021-02
- Subjects: Vigilance committees , Criminal justice, Administration of
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/22000 , vital:51928
- Description: Mob justice is a major issue that will continue to occur as long as criminal activities exist. In South Africa, mob justice has become a major challenge for the government, particularly the South African Police Service as well as the society at large. The South African Police Service (SAPS) has partnered with all relevant role-players in the fight against mob justice and other community problems which threaten the safety of inhabitants. However, concerted efforts have also been made by the government to assist the SAPS to address the occurrence and re-occurrence of mob justice by introducing strategies such as visible policing but the problem seems to persist. The study, thus, analyzed the challenges faced by the police that lead to their ineffectiveness in eliminating mob justice. This thesis draws from two theories, namely Structural Violence theory pioneered by Galtung (1969) and Just World Theory (JWT) by Adelstein (1999) to explore the challenges faced by the police in Gqeberha. This study was conducted in the Eastern Cape Province, in Gqeberha within four townships, namely Kwadwesi, Kwazakhele, Motherwell, and New Brighton. The findings gathered by utilizing Focus Group Interviews (FGIs) and semi-structured interviews with a total number of forty-eight (48) respondents comprising of sixteen (16) SAPS members, eight (08) Community Policing Forum (CPF) representatives, four (04) community leaders and twenty (20) community members. This is a qualitative research project in which participants were chosen using purposive sampling. Participants were chosen based on their knowledge and experiences relating to the phenomena being explored. The aim was to gain an in-depth understanding of the challenges encountered by the police in combating mob justice to discover some techniques to improve and strengthen the police service to make communities safer. To analyse the data thematic analysis was utilized. The results of the study indicate that an increase in mob justice in Gqeberha has been reported to be a result of the high rate of crime. However, the fact that people know they will not get arrested for participating in mob justice is one of the concerns that have led to an escalation in mob justice. The study demonstrates that in most incidents the community members have once witnessed in their respective areas, the victims were killed while few survived and in all those incidents only a few arrests were made and in those few arrests, no convictions were made. Based on this issue of arrests, results indicate that the SAPS members are confronting some difficulties in identifying and arresting the perpetrators of mob justice. The reluctance of community members to report mob justice cases and to provide information was found to be the main challenge faced by the police in combating mob justice in Motherwell Cluster precincts. These challenges have been reported to negatively affect police work performance. Further analysis revealed that CPF, community members and community leaders as part of stakeholders who are working together with SAPS are also facing some challenges in addressing this issue of mob justice. The results also indicate that mob justice does not only harm members of the community who are subjected to these occurrences, but also the police, the victims and the perpetrators themselves. Evidence-based techniques to help eradicate mob justice incidents were developed in response to this problem of mob justice. The participants suggested that the police must be provided with adequate manpower, more community patrols and vehicles. It is believed that if the police could act in time and arrest the suspected criminals, a large number of people would be rescued from being killed in mob justice. The respondents also indicated that the police must build good relations with the communities by being visible and transparent in communities. Furthermore, the SAPS participants alluded that when people are sentenced, a community engagement needs to be conducted before those people are released outside as parolees. Other respondents mentioned the issue of education, proposing that the Criminal Justice System (CJS) should frequently conduct awareness campaigns, imbizos and public meetings to teach and inform people about its operations. , Thesis (PhD) -- Faculty of Social Sciences and Humanities, 2021
- Full Text:
- Date Issued: 2021-02
Democratic citizenship in mathematics teachers’ preparation in South African Universities
- Olawale, Babawande Emmanuel https://orcid.org/ 0000-0001-5265-1583
- Authors: Olawale, Babawande Emmanuel https://orcid.org/ 0000-0001-5265-1583
- Date: 2021-02
- Subjects: Citizenship -- Study and teaching , Authoritarianism , Mathematics -- Study and teaching
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/20079 , vital:45107
- Description: Promoting a clearer understanding of the role of schools in developing democratic dispositions among young citizens is a matter of importance across the world. This is because school is believed to be the best place where future free and democratic citizens can be prepared. Similarly, it is a place where young people are equipped with the necessary skills and knowledge that will assist them in deliberating democratically, developing a better understanding of political structures and economic and world history, and learning the relevant numeracy, literacy and critical thinking skills. Unfortunately, current teacher education programmes appear to represent potential stumbling blocks to schooling playing a greater role in educating the youth for democracy and democratic citizenship. This study explored two contradictory theoretical roles for mathematics teacher education programmes, in relation to power and control in education and society. The first is that, in reality, mathematics teacher education is essentially re-productive, in that its authoritarian relationship does not encourage confrontational discussion or critique, or challenge existing and dominant practices. The second is that, in a world where democracy is increasingly becoming a global phenomenon central to development, teacher education is (or should be) essentially democratic in nature. Thus, underpinned by the interpretivist paradigm, this study employed a qualitative research approach. It also utilised a case study design as a strategy for data collection, in order to explore how the mathematics teacher education programmes in South African universities prepare learners for democratic citizenship. As such, three universities in two provinces in South Africa participated in the study, and data were collected through interviews, observation and document reviews. At each institution, interviews were carried out with mathematics teacher educators and pre-service mathematics student teachers in their second to fourth year of study. Similarly, observations took place in different teaching sessions at each institution, and in different formats. Relevant documents were also reviewed for the study. The research findings revealed that the concepts of democracy and democratic citizenship are multifaceted. The participants’ understanding of these concepts was influenced by their individual expectations, as well as their social, political and economic environments, among many other factors. In addition, it was evident that mathematics teacher education programmes play a significant role in developing socially active citizens and democratic mathematics teachers. The research findings revealed, however, that contradictions exist between pedagogical approaches that are capable of developing democratic citizens, mathematics teacher educators’ ideal image of their practices within the classroom, and the participants’ conception of mathematics. As a result, no engagement occurs in social and critical pedagogy within mathematics education classrooms, to allow learners to view mathematics as a tool for confronting issues of justice and inequality in their community and the world at large. The research findings also revealed that the practices of the main structures which are tasked with the responsibility of promoting democratic practices within higher education institutions seem ineffective, as a result of favouritism and an autocratic approach to decision making. Based on the research findings, a recommendation is made that mathematics educators democratise their classrooms, in order to develop democratic citizens who are committed to democratic principles. Another recommendation is that, in their mathematics classrooms, educators use a problem-posing pedagogical approach which is capable of challenging power structures, thereby empowering learners as users of mathematics. This will not be possible without a professional development programme guiding mathematics teacher educators in establishing and managing their democratic classrooms. Similarly, it is recommended that student representative councils, as the structures that are best known for supporting democratic citizenship at universities, strive to follow the basic principles of democratic governance. Finally, the researcher concluded that mathematics education and the task of preparing mathematics student teachers for democratic citizenship are of the utmost importance, if South Africans are to cultivate and maintain a democracy that contributes to a culture which fundamentally supports democratic principles. , Thesis (PhD) -- Faculty of Education, 2021
- Full Text:
- Date Issued: 2021-02
- Authors: Olawale, Babawande Emmanuel https://orcid.org/ 0000-0001-5265-1583
- Date: 2021-02
- Subjects: Citizenship -- Study and teaching , Authoritarianism , Mathematics -- Study and teaching
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/20079 , vital:45107
- Description: Promoting a clearer understanding of the role of schools in developing democratic dispositions among young citizens is a matter of importance across the world. This is because school is believed to be the best place where future free and democratic citizens can be prepared. Similarly, it is a place where young people are equipped with the necessary skills and knowledge that will assist them in deliberating democratically, developing a better understanding of political structures and economic and world history, and learning the relevant numeracy, literacy and critical thinking skills. Unfortunately, current teacher education programmes appear to represent potential stumbling blocks to schooling playing a greater role in educating the youth for democracy and democratic citizenship. This study explored two contradictory theoretical roles for mathematics teacher education programmes, in relation to power and control in education and society. The first is that, in reality, mathematics teacher education is essentially re-productive, in that its authoritarian relationship does not encourage confrontational discussion or critique, or challenge existing and dominant practices. The second is that, in a world where democracy is increasingly becoming a global phenomenon central to development, teacher education is (or should be) essentially democratic in nature. Thus, underpinned by the interpretivist paradigm, this study employed a qualitative research approach. It also utilised a case study design as a strategy for data collection, in order to explore how the mathematics teacher education programmes in South African universities prepare learners for democratic citizenship. As such, three universities in two provinces in South Africa participated in the study, and data were collected through interviews, observation and document reviews. At each institution, interviews were carried out with mathematics teacher educators and pre-service mathematics student teachers in their second to fourth year of study. Similarly, observations took place in different teaching sessions at each institution, and in different formats. Relevant documents were also reviewed for the study. The research findings revealed that the concepts of democracy and democratic citizenship are multifaceted. The participants’ understanding of these concepts was influenced by their individual expectations, as well as their social, political and economic environments, among many other factors. In addition, it was evident that mathematics teacher education programmes play a significant role in developing socially active citizens and democratic mathematics teachers. The research findings revealed, however, that contradictions exist between pedagogical approaches that are capable of developing democratic citizens, mathematics teacher educators’ ideal image of their practices within the classroom, and the participants’ conception of mathematics. As a result, no engagement occurs in social and critical pedagogy within mathematics education classrooms, to allow learners to view mathematics as a tool for confronting issues of justice and inequality in their community and the world at large. The research findings also revealed that the practices of the main structures which are tasked with the responsibility of promoting democratic practices within higher education institutions seem ineffective, as a result of favouritism and an autocratic approach to decision making. Based on the research findings, a recommendation is made that mathematics educators democratise their classrooms, in order to develop democratic citizens who are committed to democratic principles. Another recommendation is that, in their mathematics classrooms, educators use a problem-posing pedagogical approach which is capable of challenging power structures, thereby empowering learners as users of mathematics. This will not be possible without a professional development programme guiding mathematics teacher educators in establishing and managing their democratic classrooms. Similarly, it is recommended that student representative councils, as the structures that are best known for supporting democratic citizenship at universities, strive to follow the basic principles of democratic governance. Finally, the researcher concluded that mathematics education and the task of preparing mathematics student teachers for democratic citizenship are of the utmost importance, if South Africans are to cultivate and maintain a democracy that contributes to a culture which fundamentally supports democratic principles. , Thesis (PhD) -- Faculty of Education, 2021
- Full Text:
- Date Issued: 2021-02
Enhancing the psychological strength of adolescent rugby players in South African secondary schools : the effects of Rational-Emotive Behavioural Therapy and Mindfulness-Acceptance-Commitment Therapy
- Munnik, James Barry https://orcid.org/0000-0001-8211-4260
- Authors: Munnik, James Barry https://orcid.org/0000-0001-8211-4260
- Date: 2021-02
- Subjects: Rational emotive behavior therapy , Acceptance and commitment therapy , Psychotherapy
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/22111 , vital:51984
- Description: This thesis aimed, firstly, to test the psychological effects of two psychological interventions designed to enhance the psychological strength of competitive athletes; and, secondly, to possibly determine which intervention might be considered superior to the other. The third aim of this thesis was to explore the results of the findings in terms of both their shared and differential theories. The two psychological approaches in question were Rational-Emotive Behavioural Therapy (REBT) – supplemented with adjunctive Mental Skills Training (MST) techniques and the Mindfulness-Acceptance-Commitment (MAC) approach. To test the psychological strengthening effects of both interventions and to compare the results, a non-equivalent pre-test post-test control group design was followed which utilised a control group along with purposive sampling. Various dependent variables were investigated using seven instruments. Variables tested included: - mental wellbeing, irrational beliefs, mental toughness, achievement motivation and competitive anxiety, as well as anger and frustration levels and subjective performance. The instruments used to capture these variables were: - The Mental Health Continuum-Short Form (MHC-SF); Shortened General Attitudes and Beliefs Scale (SGABS); the Sport Mental Toughness Questionnaire (SMTQ); the Sport Competition Anxiety Test (SCAT); as well as one five-point Likert question written by the researcher and one question from the Psychological Skills Inventory (PSI). Seventy-one male adolescent rugby players, from three different schools were sourced to take part in this study. Thus 32 (Mage = 17.33; SD = 6= .729) participants from one high school completed the seven-session REBT-Based MST intervention. 21 (Mage = 17.23; SD = .669) from another school completed the seven-module MAC intervention and 18 (Mage = 15.52; SD = .326) from an additional school were used as a control group school. All three group participants filled out the instrument questionnaires prior to undergoing the various interventions and just following it. Each intervention’s sessions were about an hour to an hour and fifteen minutes long. The control group just filled out the instrument questionnaires in about the same time frame that the other schools had their pre- and post-intervention assessments. The results demonstrated that generally both interventions improved the psychological strength of their players more so than the control group, however, the REBT intervention decreased irrationality and improved subjective performance measures more significantly when compared to the other treatment group. , Thesis (PhD) -- Faculty of Social Sciences and Humanities, 2021
- Full Text:
- Date Issued: 2021-02
- Authors: Munnik, James Barry https://orcid.org/0000-0001-8211-4260
- Date: 2021-02
- Subjects: Rational emotive behavior therapy , Acceptance and commitment therapy , Psychotherapy
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/22111 , vital:51984
- Description: This thesis aimed, firstly, to test the psychological effects of two psychological interventions designed to enhance the psychological strength of competitive athletes; and, secondly, to possibly determine which intervention might be considered superior to the other. The third aim of this thesis was to explore the results of the findings in terms of both their shared and differential theories. The two psychological approaches in question were Rational-Emotive Behavioural Therapy (REBT) – supplemented with adjunctive Mental Skills Training (MST) techniques and the Mindfulness-Acceptance-Commitment (MAC) approach. To test the psychological strengthening effects of both interventions and to compare the results, a non-equivalent pre-test post-test control group design was followed which utilised a control group along with purposive sampling. Various dependent variables were investigated using seven instruments. Variables tested included: - mental wellbeing, irrational beliefs, mental toughness, achievement motivation and competitive anxiety, as well as anger and frustration levels and subjective performance. The instruments used to capture these variables were: - The Mental Health Continuum-Short Form (MHC-SF); Shortened General Attitudes and Beliefs Scale (SGABS); the Sport Mental Toughness Questionnaire (SMTQ); the Sport Competition Anxiety Test (SCAT); as well as one five-point Likert question written by the researcher and one question from the Psychological Skills Inventory (PSI). Seventy-one male adolescent rugby players, from three different schools were sourced to take part in this study. Thus 32 (Mage = 17.33; SD = 6= .729) participants from one high school completed the seven-session REBT-Based MST intervention. 21 (Mage = 17.23; SD = .669) from another school completed the seven-module MAC intervention and 18 (Mage = 15.52; SD = .326) from an additional school were used as a control group school. All three group participants filled out the instrument questionnaires prior to undergoing the various interventions and just following it. Each intervention’s sessions were about an hour to an hour and fifteen minutes long. The control group just filled out the instrument questionnaires in about the same time frame that the other schools had their pre- and post-intervention assessments. The results demonstrated that generally both interventions improved the psychological strength of their players more so than the control group, however, the REBT intervention decreased irrationality and improved subjective performance measures more significantly when compared to the other treatment group. , Thesis (PhD) -- Faculty of Social Sciences and Humanities, 2021
- Full Text:
- Date Issued: 2021-02
Reinforcing the protection of stakeholders’ interests under the South African takeover regulation regime: a comparative assessment from a complementary regulatory perspective.
- Authors: Mudzamiri, Justice
- Date: 2021-02
- Subjects: Social responsibility of business , Stockholder wealth , Corporate governance--Law and legislation
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/20350 , vital:45658
- Description: The dominant view in company law (especially; corporate governance and finance law) is that the regulation of company takeovers (takeovers) and-/ or mergers must carefully balance two opposing notions. On one hand, the regime must be designed to enable or facilitate the initiation and successful implementation of takeovers and mergers in the interests of inter alia economic growth and technological advancement. On the other hand, such a regulatory framework ought to be sensitive to stakeholders’ interests. Various policy rationales are put forward in supporting the incidence of takeover transactions. These motivations include the need for companies to access business synergy, diversification, competitiveness, technological advancement, and broader economic development. However, takeovers may have negative implications for stakeholders. For feasibility sake, this study’s focus is limited to three stakeholder groups, namely, the target company shareholders, the target company directors, and the local communities. For the target shareholders, the takeover-related mischiefs include the possibility that the target directors may be tainted by conflicts of interest in the context of an offer, thereby making recommendations that disadvantage the shareholders. Or the possibility that the minority shareholders may be treated unfairly and unequally by the acquiring company through making a subsequent offer that is inferior to the one received by the majority holders of securities of the same class. For the board of directors, there are twin negative effects that the directors may face. On the one hand, is litigation from disgruntled stakeholders during and after takeovers and, on the other hand, is the possibility that directors often lose their offices and jobs after successful takeovers. This study also examines the possible exposure of local communities to the negative repercussions of takeovers, and these include loss of employment by locals, loss of beneficial community development, loss of community development monies due to losses in corporate taxes, loss of corporate social responsibility benefits where the merged company decides to relocate. Still, the introduction of a new company into a community after a takeover may negatively impact the environment, public health as well as expose the community to severe national security threats especially where the takeovers involve personal data storage, the internet and technology. Against the backdrop of the conceivable benefits and adverse effects surrounding takeovers this study introduces a ‘novel’ complementary regulatory perspective, as a yardstick for undertaking a comparative evaluation of the existing takeover regulation regimes of the United States of America (US) especially the state of Delaware, the United Kingdom (UK) and South Africa to answer this study’s main research question. The primary question sought to be answered is: To what extent are the provisions of the South African takeover regulation framework appropriate and adequate in protecting the stakeholders’ interests? The said complementary regulatory perspective has twin-legs designed to carefully balance two opposing philosophies: that is, on one hand, vigilant optimisation of takeover activity and on the other hand, ensuring the appropriate and adequate protection of stakeholders’ interests by pursuing stakeholder inclusivity through the concept of subordination. Notably, there are several protections under the US, the UK and South African takeover regulation regimes that are available and accessible to the three stakeholder groups identified, discussed and evaluated in this study. And through the evaluations, the related merits and weaknesses of such protections were established. Then, ultimately, several suggestions for law reform are recommended in accordance with the ethos of the complementary regulatory perspective as deliberated. , Thesis (PhD) (Law)-- University of Fort Hare, 2021
- Full Text:
- Date Issued: 2021-02
- Authors: Mudzamiri, Justice
- Date: 2021-02
- Subjects: Social responsibility of business , Stockholder wealth , Corporate governance--Law and legislation
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/20350 , vital:45658
- Description: The dominant view in company law (especially; corporate governance and finance law) is that the regulation of company takeovers (takeovers) and-/ or mergers must carefully balance two opposing notions. On one hand, the regime must be designed to enable or facilitate the initiation and successful implementation of takeovers and mergers in the interests of inter alia economic growth and technological advancement. On the other hand, such a regulatory framework ought to be sensitive to stakeholders’ interests. Various policy rationales are put forward in supporting the incidence of takeover transactions. These motivations include the need for companies to access business synergy, diversification, competitiveness, technological advancement, and broader economic development. However, takeovers may have negative implications for stakeholders. For feasibility sake, this study’s focus is limited to three stakeholder groups, namely, the target company shareholders, the target company directors, and the local communities. For the target shareholders, the takeover-related mischiefs include the possibility that the target directors may be tainted by conflicts of interest in the context of an offer, thereby making recommendations that disadvantage the shareholders. Or the possibility that the minority shareholders may be treated unfairly and unequally by the acquiring company through making a subsequent offer that is inferior to the one received by the majority holders of securities of the same class. For the board of directors, there are twin negative effects that the directors may face. On the one hand, is litigation from disgruntled stakeholders during and after takeovers and, on the other hand, is the possibility that directors often lose their offices and jobs after successful takeovers. This study also examines the possible exposure of local communities to the negative repercussions of takeovers, and these include loss of employment by locals, loss of beneficial community development, loss of community development monies due to losses in corporate taxes, loss of corporate social responsibility benefits where the merged company decides to relocate. Still, the introduction of a new company into a community after a takeover may negatively impact the environment, public health as well as expose the community to severe national security threats especially where the takeovers involve personal data storage, the internet and technology. Against the backdrop of the conceivable benefits and adverse effects surrounding takeovers this study introduces a ‘novel’ complementary regulatory perspective, as a yardstick for undertaking a comparative evaluation of the existing takeover regulation regimes of the United States of America (US) especially the state of Delaware, the United Kingdom (UK) and South Africa to answer this study’s main research question. The primary question sought to be answered is: To what extent are the provisions of the South African takeover regulation framework appropriate and adequate in protecting the stakeholders’ interests? The said complementary regulatory perspective has twin-legs designed to carefully balance two opposing philosophies: that is, on one hand, vigilant optimisation of takeover activity and on the other hand, ensuring the appropriate and adequate protection of stakeholders’ interests by pursuing stakeholder inclusivity through the concept of subordination. Notably, there are several protections under the US, the UK and South African takeover regulation regimes that are available and accessible to the three stakeholder groups identified, discussed and evaluated in this study. And through the evaluations, the related merits and weaknesses of such protections were established. Then, ultimately, several suggestions for law reform are recommended in accordance with the ethos of the complementary regulatory perspective as deliberated. , Thesis (PhD) (Law)-- University of Fort Hare, 2021
- Full Text:
- Date Issued: 2021-02
South African women in politics: the case study of Buffalo City Metropolitan Municipality of the Eastern Cape Province, South Africa
- Sokani, Andile https://orcid.org/0000-0002-3835-3501
- Authors: Sokani, Andile https://orcid.org/0000-0002-3835-3501
- Date: 2021-02
- Subjects: Women -- Political activity -- South Africa , Women government executives
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/22340 , vital:52026
- Description: Many barriers exist for women to participating in local or national politics and decisionmaking. This study, which was conducted in the Buffalo City Metropolitan Municipality of South Africa's Eastern Cape Province, examines and highlights the extent to which South Africa has provided opportunities for women to participate in local politics and decisionmaking in local government. According to research, women face gender discrimination in local politics, particularly in provinces such as the Eastern Cape, where patriarchy still exists. Despite the fact that much work has been done in South Africa to combat gender discrimination, including in politics, women continue to be under-represented in local politics and decision-making. The study was founded on Feminist theory to draw attention to the issue of women's under-representation in politics. Feminists believe that the benefits and burdens of social, economic, and political life are gendered. According to feminist theory, the gender-power-order is always present in our society. The notion that women and their situations are central to political analysis is a central feature of feminist political theory, with its interest in questioning the fact that men appear to have more power and privilege than women and how this can be changed. The mixed-methods research approach was used in the study, and 200 members of the community were given questionnaires. Ten in-depth interviews with female Proportional Representation and Ward Councillors from the Buffalo City Metropolitan Municipality in South Africa's Eastern Cape Province were conducted. The study's data were analysed using the Statistical Package for Social Science (SPSS) for quantitative data and NVivo 12 Pro for qualitative data. Based on the study's theoretical frameworks, the findings revealed that, despite national and international policy and legislative provisions on women's empowerment and gender equality, women continue to face institutional gendered challenges. The researcher observed that, while there are policies encouraging women to participate in politics, the implementation of those policies is not uniform, according to those who participated in the study. The findings argued that existing policies should be implemented in order for women to participate in politics. The government must ensure that these policies are implemented so that women can fully participate in politics. The study's findings revealed that there are ongoing gender dynamics, stereotypes, and perceptions of women in politics and decision-making in local municipalities, particularly in Buffalo City Metropolitan Municipality. According to the study's findings, women should be empowered in the right way. The study also recommended that young females be educated about politics in order to increase their participation in politics. Furthermore, the study suggested that political parties in BCM should encourage women to participate in politics in their constitutions. Political parties in BCM should prioritize women because they are a direct pathway for women to elected office and political leadership. Finally, the study advocated for a greater effort to break down traditional stereotypes and prejudices that make women's ascension and retention in positions of authority difficult. , Thesis (PhD) -- Faculty of Social Sciences and Humanities, 2021
- Full Text:
- Date Issued: 2021-02
- Authors: Sokani, Andile https://orcid.org/0000-0002-3835-3501
- Date: 2021-02
- Subjects: Women -- Political activity -- South Africa , Women government executives
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/22340 , vital:52026
- Description: Many barriers exist for women to participating in local or national politics and decisionmaking. This study, which was conducted in the Buffalo City Metropolitan Municipality of South Africa's Eastern Cape Province, examines and highlights the extent to which South Africa has provided opportunities for women to participate in local politics and decisionmaking in local government. According to research, women face gender discrimination in local politics, particularly in provinces such as the Eastern Cape, where patriarchy still exists. Despite the fact that much work has been done in South Africa to combat gender discrimination, including in politics, women continue to be under-represented in local politics and decision-making. The study was founded on Feminist theory to draw attention to the issue of women's under-representation in politics. Feminists believe that the benefits and burdens of social, economic, and political life are gendered. According to feminist theory, the gender-power-order is always present in our society. The notion that women and their situations are central to political analysis is a central feature of feminist political theory, with its interest in questioning the fact that men appear to have more power and privilege than women and how this can be changed. The mixed-methods research approach was used in the study, and 200 members of the community were given questionnaires. Ten in-depth interviews with female Proportional Representation and Ward Councillors from the Buffalo City Metropolitan Municipality in South Africa's Eastern Cape Province were conducted. The study's data were analysed using the Statistical Package for Social Science (SPSS) for quantitative data and NVivo 12 Pro for qualitative data. Based on the study's theoretical frameworks, the findings revealed that, despite national and international policy and legislative provisions on women's empowerment and gender equality, women continue to face institutional gendered challenges. The researcher observed that, while there are policies encouraging women to participate in politics, the implementation of those policies is not uniform, according to those who participated in the study. The findings argued that existing policies should be implemented in order for women to participate in politics. The government must ensure that these policies are implemented so that women can fully participate in politics. The study's findings revealed that there are ongoing gender dynamics, stereotypes, and perceptions of women in politics and decision-making in local municipalities, particularly in Buffalo City Metropolitan Municipality. According to the study's findings, women should be empowered in the right way. The study also recommended that young females be educated about politics in order to increase their participation in politics. Furthermore, the study suggested that political parties in BCM should encourage women to participate in politics in their constitutions. Political parties in BCM should prioritize women because they are a direct pathway for women to elected office and political leadership. Finally, the study advocated for a greater effort to break down traditional stereotypes and prejudices that make women's ascension and retention in positions of authority difficult. , Thesis (PhD) -- Faculty of Social Sciences and Humanities, 2021
- Full Text:
- Date Issued: 2021-02
Succession politics and state administration in Africa: the case of Zimbabwe
- Authors: Chikerema, Arthur Fidelis
- Date: 2021-02
- Subjects: Africa -- Politics and government , Public administration , state governments
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/21355 , vital:48494
- Description: The research probes the impact of succession politics on state administration in Africa, Zimbabwe as a case study. Power transition in any political landscape promotes the regeneration and configuration of the political architecture and the functionality of the administrative apparatus of the state. Government performance may thus be viewed as a result of political processes and dynamics that influence governance in a polity. Political succession determines the composition of administrative organs of the state and many political leaders wish to work with administrative apparatus, which are compatible with their ideological orientation. Regular transfer of executive power is the major test of stability, legitimacy and maturity of a nation’s political system, but in Zimbabwe, political leaders have shown a consistent trajectory of violating or amending state and party constitutions to prolong their stay in power (Constitutional coup or a third term tragedy). This has been compelled by ruthless silencing of dissent by suffocating political activism culminating in strong personalities and weak institutions. Succession politics in Zimbabwe resembles executive dominance, egocentrism, excessive appointing powers which are compounded by lack of institutional framework for succession. This undermines the professional independence of the bureaucracy, which in turn inhibits the pursuit of sound governance. Therefore, passing the baton to the next leader has been a challenge; resulting in the personalization of, governments and political parties by leaders. While constitutions provide for elective congress for purposes of electing party leadership and/ or renewing their mandates, there is strong literature pointing to the capture of representative institutions such as Parliaments through strong normative values by leaders. This erodes the role of Parliaments or congresses as a democratic institution, as leaders have used it to “endorse” themselves. The research examines the influence of succession politics on state administration, especially on how the institution of the state and party politics in Zimbabwe conflate with the public administration system leading to state malfunction. This research adopts a qualitative research design that provides an understanding of underlying reasons, opinions, and motivations on political succession and state administration in Zimbabwe. Data was collected through in-depth interviews, complemented by document review and analyzed through thematic content analysis. The study found out that, despite the existence of the legal frameworks that promote seamless power transition, political, societal and historical factors exacerbate Zimbabwe’s succession dilemma. Colonial legacy, societal laxity, the military factor, capture of state institutions by the political elite, lack of a retirement institutional mechanism, the principle of sovereignty and politicization of the succession process were found as the major hindrances to Zimbabwe’s succession trajectory. It recommends broad based and genuine civil service, electoral, security and political (politics with ethics and values) reforms to dismantle the current legacy imbedded in the political system. , Thesis (PhD) (Public Administration) -- University of Fort Hare, 2021
- Full Text:
- Date Issued: 2021-02
- Authors: Chikerema, Arthur Fidelis
- Date: 2021-02
- Subjects: Africa -- Politics and government , Public administration , state governments
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/21355 , vital:48494
- Description: The research probes the impact of succession politics on state administration in Africa, Zimbabwe as a case study. Power transition in any political landscape promotes the regeneration and configuration of the political architecture and the functionality of the administrative apparatus of the state. Government performance may thus be viewed as a result of political processes and dynamics that influence governance in a polity. Political succession determines the composition of administrative organs of the state and many political leaders wish to work with administrative apparatus, which are compatible with their ideological orientation. Regular transfer of executive power is the major test of stability, legitimacy and maturity of a nation’s political system, but in Zimbabwe, political leaders have shown a consistent trajectory of violating or amending state and party constitutions to prolong their stay in power (Constitutional coup or a third term tragedy). This has been compelled by ruthless silencing of dissent by suffocating political activism culminating in strong personalities and weak institutions. Succession politics in Zimbabwe resembles executive dominance, egocentrism, excessive appointing powers which are compounded by lack of institutional framework for succession. This undermines the professional independence of the bureaucracy, which in turn inhibits the pursuit of sound governance. Therefore, passing the baton to the next leader has been a challenge; resulting in the personalization of, governments and political parties by leaders. While constitutions provide for elective congress for purposes of electing party leadership and/ or renewing their mandates, there is strong literature pointing to the capture of representative institutions such as Parliaments through strong normative values by leaders. This erodes the role of Parliaments or congresses as a democratic institution, as leaders have used it to “endorse” themselves. The research examines the influence of succession politics on state administration, especially on how the institution of the state and party politics in Zimbabwe conflate with the public administration system leading to state malfunction. This research adopts a qualitative research design that provides an understanding of underlying reasons, opinions, and motivations on political succession and state administration in Zimbabwe. Data was collected through in-depth interviews, complemented by document review and analyzed through thematic content analysis. The study found out that, despite the existence of the legal frameworks that promote seamless power transition, political, societal and historical factors exacerbate Zimbabwe’s succession dilemma. Colonial legacy, societal laxity, the military factor, capture of state institutions by the political elite, lack of a retirement institutional mechanism, the principle of sovereignty and politicization of the succession process were found as the major hindrances to Zimbabwe’s succession trajectory. It recommends broad based and genuine civil service, electoral, security and political (politics with ethics and values) reforms to dismantle the current legacy imbedded in the political system. , Thesis (PhD) (Public Administration) -- University of Fort Hare, 2021
- Full Text:
- Date Issued: 2021-02
Succession politics and state administration in Africa: the case of Zimbabwe.
- Authors: Chikerema, Arthur Fidelis
- Date: 2021-02
- Subjects: Public administration , State governments
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/21467 , vital:48659
- Description: The research probes the impact of succession politics on state administration in Africa, Zimbabwe as a case study. Power transition in any political landscape promotes the regeneration and configuration of the political architecture and the functionality of the administrative apparatus of the state. Government performance may thus be viewed as a result of political processes and dynamics that influence governance in a polity. Political succession determines the composition of administrative organs of the state and many political leaders wish to work with administrative apparatus, which are compatible with their ideological orientation. Regular transfer of executive power is the major test of stability, legitimacy and maturity of a nation’s political system, but in Zimbabwe, political leaders have shown a consistent trajectory of violating or amending state and party constitutions to prolong their stay in power (Constitutional coup or a third term tragedy). This has been compelled by ruthless silencing of dissent by suffocating political activism culminating in strong personalities and weak institutions. Succession politics in Zimbabwe resembles executive dominance, egocentrism, excessive appointing powers which are compounded by lack of institutional framework for succession. This undermines the professional independence of the bureaucracy, which in turn inhibits the pursuit of sound governance. Therefore, passing the baton to the next leader has been a challenge; resulting in the personalization of, governments and political parties by leaders. While constitutions provide for elective congress for purposes of electing party leadership and/ or renewing their mandates, there is strong literature pointing to the capture of representative institutions such as Parliaments through strong normative values by leaders. This erodes the role of Parliaments or congresses as a democratic institution, as leaders have used it to “endorse” themselves. The research examines the influence of succession politics on state administration, especially on how the institution of the state and party politics in Zimbabwe conflate with the public administration system leading to state malfunction. This research adopts a qualitative research design that provides an understanding of underlying reasons, opinions, and motivations on political succession and state administration in Zimbabwe. Data was collected through in-depth interviews, complemented by document review and analyzed through thematic content analysis. The study found out that, despite the existence of the legal frameworks that promote seamless power transition, political, societal and historical factors exacerbate Zimbabwe’s succession dilemma. Colonial legacy, societal laxity, the military factor, capture of state institutions by the political elite, lack of a retirement institutional mechanism, the principle of sovereignty and politicization of the succession process were found as the major hindrances to Zimbabwe’s succession trajectory. It recommends broad based and genuine civil service, electoral, security and political (politics with ethics and values) reforms to dismantle the current legacy imbedded in the political system. , Thesis (PhD) (Public Administration) -- University of Fort Hare, 2021
- Full Text:
- Date Issued: 2021-02
- Authors: Chikerema, Arthur Fidelis
- Date: 2021-02
- Subjects: Public administration , State governments
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/21467 , vital:48659
- Description: The research probes the impact of succession politics on state administration in Africa, Zimbabwe as a case study. Power transition in any political landscape promotes the regeneration and configuration of the political architecture and the functionality of the administrative apparatus of the state. Government performance may thus be viewed as a result of political processes and dynamics that influence governance in a polity. Political succession determines the composition of administrative organs of the state and many political leaders wish to work with administrative apparatus, which are compatible with their ideological orientation. Regular transfer of executive power is the major test of stability, legitimacy and maturity of a nation’s political system, but in Zimbabwe, political leaders have shown a consistent trajectory of violating or amending state and party constitutions to prolong their stay in power (Constitutional coup or a third term tragedy). This has been compelled by ruthless silencing of dissent by suffocating political activism culminating in strong personalities and weak institutions. Succession politics in Zimbabwe resembles executive dominance, egocentrism, excessive appointing powers which are compounded by lack of institutional framework for succession. This undermines the professional independence of the bureaucracy, which in turn inhibits the pursuit of sound governance. Therefore, passing the baton to the next leader has been a challenge; resulting in the personalization of, governments and political parties by leaders. While constitutions provide for elective congress for purposes of electing party leadership and/ or renewing their mandates, there is strong literature pointing to the capture of representative institutions such as Parliaments through strong normative values by leaders. This erodes the role of Parliaments or congresses as a democratic institution, as leaders have used it to “endorse” themselves. The research examines the influence of succession politics on state administration, especially on how the institution of the state and party politics in Zimbabwe conflate with the public administration system leading to state malfunction. This research adopts a qualitative research design that provides an understanding of underlying reasons, opinions, and motivations on political succession and state administration in Zimbabwe. Data was collected through in-depth interviews, complemented by document review and analyzed through thematic content analysis. The study found out that, despite the existence of the legal frameworks that promote seamless power transition, political, societal and historical factors exacerbate Zimbabwe’s succession dilemma. Colonial legacy, societal laxity, the military factor, capture of state institutions by the political elite, lack of a retirement institutional mechanism, the principle of sovereignty and politicization of the succession process were found as the major hindrances to Zimbabwe’s succession trajectory. It recommends broad based and genuine civil service, electoral, security and political (politics with ethics and values) reforms to dismantle the current legacy imbedded in the political system. , Thesis (PhD) (Public Administration) -- University of Fort Hare, 2021
- Full Text:
- Date Issued: 2021-02
The term structure of interest rate and expected inflation in South Africa
- Authors: Deve, Richard
- Date: 2021-02
- Subjects: Inflation (Finance) , Rational expectations (Economic theory)| , Inflation (Finance) -- Mathematical models
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/20252 , vital:45639
- Description: One of the questions in the minds of policy-makers, monetary authorities and the government is the nature of South Africa’s yield curve and its ability to predict inflation expectations for this country. This calls for an investigation of the relationship between the term structure of interest rates and expected inflation in South Africa. The study seeks to provide answers to three sequentially structured questions: Is there a long-term relationship between the yield curve and inflation expectations in South Africa? Does interest rate term structure contain information about future inflation movements in an inflation-targeting regime for South Africa? And finally: How useful is the yield spread as a predictor for future inflation in South Africa? To shed light on these questions, monthly data for five core variables covering the period January 2000 to January 2017 was used. In South Africa, a tight monetary policy stance affects economic activity positively. The development of sounder bond markets in developed economies, which enhance economic development, creates predictable monetary policy direction relative to developing economies that are cha-racterised by unstable economic conditions coupled with policy inconsistence. This phenomenon results in less credible central banks, and hence leads to less anchored inflation expectations. However, South Africa has well-anchored inflation expectations and one of the most liquid bond markets in the world, which sets it in an excellent position to compete with developed economies. South Africa’s risk premium and inflation are less volatile than those of other developing countries. This particular study finds that a tight monetary policy stance results in an increase in short-term interest rates, leading consequently to a decline in future inflation expectations, and hence leading to a decrease in long-term interest rates, through which the credibility of the central bank is recognised. The positive marginal effect of the yield spread on inflation expectations suggests that the inflation expectation changes are influenced positively by changes in the yield spread. An observed long-run relationship exists between inflation expectations and the yield spread signals that the yield spread has predictive ability for inflation ex-pectations in South Africa. The forecast inflation mimics realised inflation, which could suggest that the forecast is fairly accurate and valuable in explaining the influence of the inflation targeting regime adopted by the South African Reserve Bank (SARB). On the other hand, inflation expectations are viewed as a lagging indicator. In this line of argument, Mishkin (1990b) mentions that past studies have used the yield curve to extract information about future inflation. This is in line with the findings of the SARB (2016). That study (2016) found that the yield spread is useful for predicting expected future yield over 24 quarters in the South African economy. The study found that the yield curve does have a predictive ability with regard to expected inflation, which is moderate. However, it was found that the repo rate and the current levels of inflation had more predictive power than the yield curve. , Thesis (DPhil) -- Faculty of Management and Commerce, 2021
- Full Text:
- Date Issued: 2021-02
- Authors: Deve, Richard
- Date: 2021-02
- Subjects: Inflation (Finance) , Rational expectations (Economic theory)| , Inflation (Finance) -- Mathematical models
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/20252 , vital:45639
- Description: One of the questions in the minds of policy-makers, monetary authorities and the government is the nature of South Africa’s yield curve and its ability to predict inflation expectations for this country. This calls for an investigation of the relationship between the term structure of interest rates and expected inflation in South Africa. The study seeks to provide answers to three sequentially structured questions: Is there a long-term relationship between the yield curve and inflation expectations in South Africa? Does interest rate term structure contain information about future inflation movements in an inflation-targeting regime for South Africa? And finally: How useful is the yield spread as a predictor for future inflation in South Africa? To shed light on these questions, monthly data for five core variables covering the period January 2000 to January 2017 was used. In South Africa, a tight monetary policy stance affects economic activity positively. The development of sounder bond markets in developed economies, which enhance economic development, creates predictable monetary policy direction relative to developing economies that are cha-racterised by unstable economic conditions coupled with policy inconsistence. This phenomenon results in less credible central banks, and hence leads to less anchored inflation expectations. However, South Africa has well-anchored inflation expectations and one of the most liquid bond markets in the world, which sets it in an excellent position to compete with developed economies. South Africa’s risk premium and inflation are less volatile than those of other developing countries. This particular study finds that a tight monetary policy stance results in an increase in short-term interest rates, leading consequently to a decline in future inflation expectations, and hence leading to a decrease in long-term interest rates, through which the credibility of the central bank is recognised. The positive marginal effect of the yield spread on inflation expectations suggests that the inflation expectation changes are influenced positively by changes in the yield spread. An observed long-run relationship exists between inflation expectations and the yield spread signals that the yield spread has predictive ability for inflation ex-pectations in South Africa. The forecast inflation mimics realised inflation, which could suggest that the forecast is fairly accurate and valuable in explaining the influence of the inflation targeting regime adopted by the South African Reserve Bank (SARB). On the other hand, inflation expectations are viewed as a lagging indicator. In this line of argument, Mishkin (1990b) mentions that past studies have used the yield curve to extract information about future inflation. This is in line with the findings of the SARB (2016). That study (2016) found that the yield spread is useful for predicting expected future yield over 24 quarters in the South African economy. The study found that the yield curve does have a predictive ability with regard to expected inflation, which is moderate. However, it was found that the repo rate and the current levels of inflation had more predictive power than the yield curve. , Thesis (DPhil) -- Faculty of Management and Commerce, 2021
- Full Text:
- Date Issued: 2021-02
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