Pharmacological, toxicological and phytochemical evaluation of helichrysum petiolare hilliard & b.l. burtt - an indigenous plant traditionally used in the treatment of diabetes in the eastern cape province of South Africa
- Aladejana, Adebowale Emmanuel https://orcid.org/0000-0003-1871-926X
- Authors: Aladejana, Adebowale Emmanuel https://orcid.org/0000-0003-1871-926X
- Date: 2022-04
- Subjects: Diabetes -- Alternative treatment , Traditional medicine , Medicinal plants
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/22787 , vital:52755
- Description: Diabetes mellitus is one of the leading causes of death in South Africa, and it has already placed significant stress on the country’s health sector and economy. The orthodox hypoglycaemic drugs are not only ineffective in the management of the disease and its complications, but they also possess unwanted side effects. The need for alternative non-toxic drugs is therefore imperative. Various studies have listed several medicinal plants that can be successfully used in the herbal treatment of diabetes and have investigated them for their anti-diabetic potentials in vivo and/or in vitro. Out of the different potential herbal species, plants belonging to the Asteraceae family possess highly potent hypoglycaemic properties with negligible toxicities. Five Asteraceae plants widely used in different parts of South Africa for the treatment of diabetes were reviewed. The review provided an update of scientific evidence on the hypoglycaemic properties of the plants. However, Helichrysum petiolare was studied extensively in this study for its antidiabetic activity H. petiolare has been listed in many ethnobotanical surveys as a plant with potent hypoglycaemic potential, this, however, has not been properly verified in scientific literature and there has hardly been any study on the essential oil and nutritional composition, and antioxidant, antidiabetic, and cytotoxicity potentials of the plant. The effects of hydro-distillation (HD) and solvent-free microwave extraction (SFME) methods on the chemical constituents of H. petiolare-derived essential oils were evaluated. The SFME method had a higher yield of essential oil than the HD. There were substantial amounts of monoterpenes, monoterpene alcohols, sesquiterpenes, and sesquiterpene alcohols in both essential oils obtained, but these compounds were more profound in the SFME derived essential oil which has 62 compounds compared to the 52 derived through HD. The SFME derived essential oil can therefore be said to be of better quality than the HD method. The compounds obtained in the essential oils have high pharmaceutical and cosmetic value, and as observed in this study, their quantity is dependent on the method of extraction (Ibáñez and Blázquez, 2021; Kaur et al., 2021). The proximate analysis of the whole plant of H. petiolare showed high levels of Acid Detergent Fibre (ADF), vitamins (A, C and E), Neutral Detergent Fibre (NDF), and minerals. The high ADF level is believed to be responsible for the low energy, fat and carbohydrate levels observed in the study. The result showed a high level of oxalate and therefore suggests cooking of the plant before human consumption. Overall nutrition and mineral compositions of the plant showed that H. petiolare is immensely rich in vital nutrients that are of great importance to health and metabolism; these nutrients are suggested to be partly responsible for the plant’s useful medicinal properties. The phytochemical contents of the acetone (ACQ), ethanol (ETQ), and boiled (BAQ) and cold (CAQ) aqueous whole-plant extracts of Helichrysum petiolare were determined using standard phytochemical reaction methods. ABTS, DPPH, NO and TAC assays were used to evaluate their antioxidant properties. The highest total phenolic content (212,963 mg/g) was reported in the BAQ extract, while the ETQ had the highest flavonoid (172.393 mg/g) and proanthocyanidin contents (65.855 mg/g). Alkaloids, flavonols, and saponin were highest in the ACQ extract, while the CAQ had the lowest phytochemical content. Among the extracts, the BAQ had the highest DPPH•+ (IC50 0.02 mg/mL) and ABTS•+ (IC50 0.07) inhibition capacities, while the ETQ exhibited the highest NO• Inhibition (IC50 0.41 mg/mL) and TAC (IC50 0.19 mg/mL). These findings justify the use of H. petiolare in traditional medicine and further recommend the ETQ and BAQ extracts of the plant as more effective extracts for medicinal treatment. The hepatotoxicity (cytotoxicity, mitotoxicity and lipotoxicity) potential of the BAQ, CAQ and ETQ extracts of Helichrysum petiolare was evaluated using standard procedures. The results showed negligible BAQ and CAQ cytotoxicities, which were further, corroborated by stability in the mitochondrial membrane potentials and were congruent with the CAQ and BAQ results for steatosis and phospholipidosis. The data suggested favourable CAQ and BAQ toxicity profiles with limited risks for hepatotoxicity. The ETQ extract, however, showed significantly high levels of cytotoxicity and lipotoxicity, and a low level of mitotoxicity. Our result suggested a potential risk of the ETQ extract for hepatotoxicity but appears partly independent of direct mitochondrial involvement. Glucose uptake assay showed significantly increased glucose uptake in the BAQ and CAQ treated L6 and C3A cell lines. The CAQ extract enhanced glucose uptake more in the L6 myocytes than in the C3A cell-lines hepatocytes. The BAQ extract showed higher levels of inhibition on α–amylase and α-glucosidase activities as compared to CAQ. The BAQ and CAQ extracts of H. petiolare may, therefore, contain pharmacologically active and relatively non-toxic hypoglycaemic chemicals, which may be effective substitutes in the treatment of diabetes mellitus. This study provides up to date scientific information on the use of H. petiolare in the treatment of diabetes mellitus in the Eastern Cape of South Africa. It justifies the use of this plant in herbal medicine and sheds more light on its previously vaguely understood nutritional and medicinal potentials. More studies, however, need to be done to isolate, identify and purify the constituent bioactive compound(s). Their dosage of application and mode of action also needs to be understood. , Thesis (PhD) -- Faculty of Science and Agriculture, 2022
- Full Text:
- Date Issued: 2022-04
- Authors: Aladejana, Adebowale Emmanuel https://orcid.org/0000-0003-1871-926X
- Date: 2022-04
- Subjects: Diabetes -- Alternative treatment , Traditional medicine , Medicinal plants
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/22787 , vital:52755
- Description: Diabetes mellitus is one of the leading causes of death in South Africa, and it has already placed significant stress on the country’s health sector and economy. The orthodox hypoglycaemic drugs are not only ineffective in the management of the disease and its complications, but they also possess unwanted side effects. The need for alternative non-toxic drugs is therefore imperative. Various studies have listed several medicinal plants that can be successfully used in the herbal treatment of diabetes and have investigated them for their anti-diabetic potentials in vivo and/or in vitro. Out of the different potential herbal species, plants belonging to the Asteraceae family possess highly potent hypoglycaemic properties with negligible toxicities. Five Asteraceae plants widely used in different parts of South Africa for the treatment of diabetes were reviewed. The review provided an update of scientific evidence on the hypoglycaemic properties of the plants. However, Helichrysum petiolare was studied extensively in this study for its antidiabetic activity H. petiolare has been listed in many ethnobotanical surveys as a plant with potent hypoglycaemic potential, this, however, has not been properly verified in scientific literature and there has hardly been any study on the essential oil and nutritional composition, and antioxidant, antidiabetic, and cytotoxicity potentials of the plant. The effects of hydro-distillation (HD) and solvent-free microwave extraction (SFME) methods on the chemical constituents of H. petiolare-derived essential oils were evaluated. The SFME method had a higher yield of essential oil than the HD. There were substantial amounts of monoterpenes, monoterpene alcohols, sesquiterpenes, and sesquiterpene alcohols in both essential oils obtained, but these compounds were more profound in the SFME derived essential oil which has 62 compounds compared to the 52 derived through HD. The SFME derived essential oil can therefore be said to be of better quality than the HD method. The compounds obtained in the essential oils have high pharmaceutical and cosmetic value, and as observed in this study, their quantity is dependent on the method of extraction (Ibáñez and Blázquez, 2021; Kaur et al., 2021). The proximate analysis of the whole plant of H. petiolare showed high levels of Acid Detergent Fibre (ADF), vitamins (A, C and E), Neutral Detergent Fibre (NDF), and minerals. The high ADF level is believed to be responsible for the low energy, fat and carbohydrate levels observed in the study. The result showed a high level of oxalate and therefore suggests cooking of the plant before human consumption. Overall nutrition and mineral compositions of the plant showed that H. petiolare is immensely rich in vital nutrients that are of great importance to health and metabolism; these nutrients are suggested to be partly responsible for the plant’s useful medicinal properties. The phytochemical contents of the acetone (ACQ), ethanol (ETQ), and boiled (BAQ) and cold (CAQ) aqueous whole-plant extracts of Helichrysum petiolare were determined using standard phytochemical reaction methods. ABTS, DPPH, NO and TAC assays were used to evaluate their antioxidant properties. The highest total phenolic content (212,963 mg/g) was reported in the BAQ extract, while the ETQ had the highest flavonoid (172.393 mg/g) and proanthocyanidin contents (65.855 mg/g). Alkaloids, flavonols, and saponin were highest in the ACQ extract, while the CAQ had the lowest phytochemical content. Among the extracts, the BAQ had the highest DPPH•+ (IC50 0.02 mg/mL) and ABTS•+ (IC50 0.07) inhibition capacities, while the ETQ exhibited the highest NO• Inhibition (IC50 0.41 mg/mL) and TAC (IC50 0.19 mg/mL). These findings justify the use of H. petiolare in traditional medicine and further recommend the ETQ and BAQ extracts of the plant as more effective extracts for medicinal treatment. The hepatotoxicity (cytotoxicity, mitotoxicity and lipotoxicity) potential of the BAQ, CAQ and ETQ extracts of Helichrysum petiolare was evaluated using standard procedures. The results showed negligible BAQ and CAQ cytotoxicities, which were further, corroborated by stability in the mitochondrial membrane potentials and were congruent with the CAQ and BAQ results for steatosis and phospholipidosis. The data suggested favourable CAQ and BAQ toxicity profiles with limited risks for hepatotoxicity. The ETQ extract, however, showed significantly high levels of cytotoxicity and lipotoxicity, and a low level of mitotoxicity. Our result suggested a potential risk of the ETQ extract for hepatotoxicity but appears partly independent of direct mitochondrial involvement. Glucose uptake assay showed significantly increased glucose uptake in the BAQ and CAQ treated L6 and C3A cell lines. The CAQ extract enhanced glucose uptake more in the L6 myocytes than in the C3A cell-lines hepatocytes. The BAQ extract showed higher levels of inhibition on α–amylase and α-glucosidase activities as compared to CAQ. The BAQ and CAQ extracts of H. petiolare may, therefore, contain pharmacologically active and relatively non-toxic hypoglycaemic chemicals, which may be effective substitutes in the treatment of diabetes mellitus. This study provides up to date scientific information on the use of H. petiolare in the treatment of diabetes mellitus in the Eastern Cape of South Africa. It justifies the use of this plant in herbal medicine and sheds more light on its previously vaguely understood nutritional and medicinal potentials. More studies, however, need to be done to isolate, identify and purify the constituent bioactive compound(s). Their dosage of application and mode of action also needs to be understood. , Thesis (PhD) -- Faculty of Science and Agriculture, 2022
- Full Text:
- Date Issued: 2022-04
Phytochemical investigation and antidiabetic activity of cissampelos capensis and strychnos henningsii the Eastern Cape medicinal plants
- Authors: Latolla, Nehemiah Solomon
- Date: 2022-04
- Subjects: Medicinal plants -- Eastern Cape (South Africa) , Endemic plants -- South Africa
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10948/56145 , vital:55956
- Description: Diabetes mellitus is recorded as a significant health crisis in South Africa and various medicinal plants are used for the management of diabetes. However, the chemistry and bioactivity associated with these plants' antidiabetic activity are still lacking. Cissampelos capensis L.f. and Strychnos henningsii Gilg are among the plants utilised to manage diabetes in the Eastern Cape Province, South Africa. C. capensis and S. henningsii are known for their isoquinoline – and indole alkaloids, respectively. Other phytochemical groups associated with antidiabetic activity are the phenols, flavonoids, and terpenes. Thus, this study aimed to evaluate in-depth the phytochemical profiling, alkaloidal identification, and structural characterisation of phytochemicals isolated from these plants. Also, subsequent in vitro antidiabetic screening of the crude extracts and isolated compounds from both plants was done. The phytochemical profiling was performed by employing a qualitative and quantitative approach through high-performance thin layer chromatography (HPTLC) and UV-spectrometry. Further analysis for the identification of alkaloids was done by using LCMS. Fractionation and purification of crude extracts were achieved through crystallisation and various chromatographic techniques. NMR, HRMS, UV/Vis, FTIR, and XRD spectroscopic techniques were used to characterise isolated compounds. In vitro antidiabetic activity screening involved the investigation of cytotoxicity, antioxidant activity, alpha-amylase – and/or alpha-glucosidase inhibition (particular to type II diabetes). C. capensis and S. henningsii extracts tested positive for alkaloids, phenolics, flavonoids, and terpenes through HPTLC screening. Quantitative estimation by UV spectrometry of these phytochemical groups detected the highest concentration of alkaloids, followed by flavonoids, phenolics, and terpenes content. The in vitro antidiabetic biological screening of the various crude extracts suggested that the presence of alkaloids, flavonoids, phenolics, and terpenes fostered favourable biological activity. The LCMS of the known alkaloidal constituents from C. capensis detected the presence of ten alkaloids, including glaziovine, lauroscholtzine, pronuciferine and vi salutardine. Seven known alkaloids and one triterpene were detected from S. henningsii, including holtsiine, 23-hdroxyspermostrychnine, henningsiine, and the triterpene, friedelin. These findings suggest that C. capensis and S. henningsii have similar phytochemical constituents compared to the species reported in literature. However, the distribution of the phytochemicals in the various plant parts differed from previous accounts. , Thesis (PhD) -- Faculty of Science,School of Biomecular and Chemical Sciences , 2022
- Full Text:
- Date Issued: 2022-04
- Authors: Latolla, Nehemiah Solomon
- Date: 2022-04
- Subjects: Medicinal plants -- Eastern Cape (South Africa) , Endemic plants -- South Africa
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10948/56145 , vital:55956
- Description: Diabetes mellitus is recorded as a significant health crisis in South Africa and various medicinal plants are used for the management of diabetes. However, the chemistry and bioactivity associated with these plants' antidiabetic activity are still lacking. Cissampelos capensis L.f. and Strychnos henningsii Gilg are among the plants utilised to manage diabetes in the Eastern Cape Province, South Africa. C. capensis and S. henningsii are known for their isoquinoline – and indole alkaloids, respectively. Other phytochemical groups associated with antidiabetic activity are the phenols, flavonoids, and terpenes. Thus, this study aimed to evaluate in-depth the phytochemical profiling, alkaloidal identification, and structural characterisation of phytochemicals isolated from these plants. Also, subsequent in vitro antidiabetic screening of the crude extracts and isolated compounds from both plants was done. The phytochemical profiling was performed by employing a qualitative and quantitative approach through high-performance thin layer chromatography (HPTLC) and UV-spectrometry. Further analysis for the identification of alkaloids was done by using LCMS. Fractionation and purification of crude extracts were achieved through crystallisation and various chromatographic techniques. NMR, HRMS, UV/Vis, FTIR, and XRD spectroscopic techniques were used to characterise isolated compounds. In vitro antidiabetic activity screening involved the investigation of cytotoxicity, antioxidant activity, alpha-amylase – and/or alpha-glucosidase inhibition (particular to type II diabetes). C. capensis and S. henningsii extracts tested positive for alkaloids, phenolics, flavonoids, and terpenes through HPTLC screening. Quantitative estimation by UV spectrometry of these phytochemical groups detected the highest concentration of alkaloids, followed by flavonoids, phenolics, and terpenes content. The in vitro antidiabetic biological screening of the various crude extracts suggested that the presence of alkaloids, flavonoids, phenolics, and terpenes fostered favourable biological activity. The LCMS of the known alkaloidal constituents from C. capensis detected the presence of ten alkaloids, including glaziovine, lauroscholtzine, pronuciferine and vi salutardine. Seven known alkaloids and one triterpene were detected from S. henningsii, including holtsiine, 23-hdroxyspermostrychnine, henningsiine, and the triterpene, friedelin. These findings suggest that C. capensis and S. henningsii have similar phytochemical constituents compared to the species reported in literature. However, the distribution of the phytochemicals in the various plant parts differed from previous accounts. , Thesis (PhD) -- Faculty of Science,School of Biomecular and Chemical Sciences , 2022
- Full Text:
- Date Issued: 2022-04
Rethinking the informal settlements upgrading programmes in the Free State Province, South Africa: a transformative and multi-faceted model
- Authors: Sive Dintsi
- Date: 2022-04
- Subjects: Squatter settlements --South Africa -- Free State , City planning South Africa --Citizen participation
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10948/55987 , vital:54561
- Description: This thesis intended to reveal the factors that contributed to the formulation of the informal settlements upgrading programmes in the Free State, South Africa, from an institutional perspective; subsequently to conceptualise a transformative and multi faceted model for the development and management of human settlements. The mixed methods exploratory research design has been applied in this study. This research design has allowed for the implementation of research methods including the qualitative inductive content analysis method, the case study method, and the semi structured survey questionnaire method. The data collection methods used comprised both primary and secondary data. Primary data involved online surveys and field observations. Secondary data comprised of an inductive analysis of written sources. The main findings of the study were as follows. First, the policy design and implementation processes have been misaligned. Second, there has been inadequate stakeholder engagements. Third, the government has not prioritised the employment of specialists in the field of housing and human settlements. Fourth, corruption has caused the state to fail to provide adequate housing for all. Fifth, municipalities are unable to identify and define existing informal settlements. Lastly, illegal immigration is causing a rapid increase in the number of informal settlements. This thesis proffered key recommendations based on the conclusions drawn from these findings. First, there must be an implementation of an integrated model for the preparation of upgrading policy. Second, communication technology education for officials of the state must be enhanced. Third, the government must prioritise the employment of experts in upgrading work. Fourth, the upgrading policy must reflect the paradigm shift from housing to human settlements. Fifth, municipalities need to be systematically transformed and improved for their functions on upgrading. Finally, illegal immigration must be firmly monitored while legal immigration is discouraged. To this end, this thesis has presented a strong viewpoint that limits the influx of immigrants into unproclaimed urban land that leads to the growth of informal settlements. The study has also strongly advocated for the legal spatial placement of South African citizens and legal immigrants on strategically and socio-economically viable land, where it is suitably available and owned by the state. Housing in the thesis has been viewed as one of the essential instruments that must inevitably emerge as a prospect towards xiv effectively unlocking improved living conditions in informal settlements; rather than housing being perceived as human settlements. , Thesis (PhD) -- Faculty of Engineering, the Built Environment and Information Technology, School of the Built Environment, 2022
- Full Text:
- Date Issued: 2022-04
- Authors: Sive Dintsi
- Date: 2022-04
- Subjects: Squatter settlements --South Africa -- Free State , City planning South Africa --Citizen participation
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10948/55987 , vital:54561
- Description: This thesis intended to reveal the factors that contributed to the formulation of the informal settlements upgrading programmes in the Free State, South Africa, from an institutional perspective; subsequently to conceptualise a transformative and multi faceted model for the development and management of human settlements. The mixed methods exploratory research design has been applied in this study. This research design has allowed for the implementation of research methods including the qualitative inductive content analysis method, the case study method, and the semi structured survey questionnaire method. The data collection methods used comprised both primary and secondary data. Primary data involved online surveys and field observations. Secondary data comprised of an inductive analysis of written sources. The main findings of the study were as follows. First, the policy design and implementation processes have been misaligned. Second, there has been inadequate stakeholder engagements. Third, the government has not prioritised the employment of specialists in the field of housing and human settlements. Fourth, corruption has caused the state to fail to provide adequate housing for all. Fifth, municipalities are unable to identify and define existing informal settlements. Lastly, illegal immigration is causing a rapid increase in the number of informal settlements. This thesis proffered key recommendations based on the conclusions drawn from these findings. First, there must be an implementation of an integrated model for the preparation of upgrading policy. Second, communication technology education for officials of the state must be enhanced. Third, the government must prioritise the employment of experts in upgrading work. Fourth, the upgrading policy must reflect the paradigm shift from housing to human settlements. Fifth, municipalities need to be systematically transformed and improved for their functions on upgrading. Finally, illegal immigration must be firmly monitored while legal immigration is discouraged. To this end, this thesis has presented a strong viewpoint that limits the influx of immigrants into unproclaimed urban land that leads to the growth of informal settlements. The study has also strongly advocated for the legal spatial placement of South African citizens and legal immigrants on strategically and socio-economically viable land, where it is suitably available and owned by the state. Housing in the thesis has been viewed as one of the essential instruments that must inevitably emerge as a prospect towards xiv effectively unlocking improved living conditions in informal settlements; rather than housing being perceived as human settlements. , Thesis (PhD) -- Faculty of Engineering, the Built Environment and Information Technology, School of the Built Environment, 2022
- Full Text:
- Date Issued: 2022-04
Scenarios for South Africa’s ocean economy towards 2060
- Authors: Hadi, Nomtha
- Date: 2022-04
- Subjects: Sustainable development--South Africa , Economic development--South Africa
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10948/57676 , vital:58195
- Description: The ocean is becoming the new focal point in the discourse on growth and sustainable development, both at national and international levels. Scenario planning improves the quality of the strategic conversations that organisations need to have as a position in preparing for the future. In this research, scenarios for South Africa’s ocean economy towards 2060 were developed and explored by considering the six key ocean sectors as outlined in the Operations Phakisa Strategy, namely, Marine Transport and Manufacturing, Offshore Oil and Gas Exploration, Aquaculture, Small Harbours, Coastal and Marine Tourism, Marine Protection Services and Ocean Governance. The research process was guided by Inayatullah’s six pillars and linked methods towards scenario development. Existing studies do not take into consideration the various social, technological, economic, environmental, political, legislative and ethical factors influencing the growth of the ocean economy. With the ocean increasingly being considered a new economic frontier, sustainable development has become pertinent for supporting and facilitating continued economic growth areas without compromising on environmental, social, or economic factors. Sustainable development within the ocean economy is further supported by Goal 14 of the Sustainable Development Goals, which focuses on conservation and sustainable use of the ocean, sea and marine resources. A literature review was conducted to gain a better understanding of the ocean economy, and primary data was collected through a real-time Delphi platform and semi-structured interviews. The real-time Delphi collected information on the key megatrends that continue to shape the ocean economy and key drivers and wild cards that can affect the sustainability of the key ocean economy industries. In addition, semi-structured interviews collected information on the proposed scenarios and the capacity needed in growing and promoting a sustainable ocean economy for South Africa towards 2060. A scenario matrix was developed for South Africa’s ocean economy and four scenarios and their implications were analysed. The preferred scenario was a resilient ocean economy towards 2060. The study also took a step forward with a view to provide reference to stakeholders and governments in progressing towards a possible scenario and developed an integrated vision for a sustainable ocean economy for South Africa towards 2060. This proposed vision is to ABSTRACT v mitigate against future challenges, collaborate and proactively utilise the development opportunities offered by the ocean economy. , Thesis (PhD) -- Faculty of Business and Economic science, 2022
- Full Text:
- Date Issued: 2022-04
- Authors: Hadi, Nomtha
- Date: 2022-04
- Subjects: Sustainable development--South Africa , Economic development--South Africa
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10948/57676 , vital:58195
- Description: The ocean is becoming the new focal point in the discourse on growth and sustainable development, both at national and international levels. Scenario planning improves the quality of the strategic conversations that organisations need to have as a position in preparing for the future. In this research, scenarios for South Africa’s ocean economy towards 2060 were developed and explored by considering the six key ocean sectors as outlined in the Operations Phakisa Strategy, namely, Marine Transport and Manufacturing, Offshore Oil and Gas Exploration, Aquaculture, Small Harbours, Coastal and Marine Tourism, Marine Protection Services and Ocean Governance. The research process was guided by Inayatullah’s six pillars and linked methods towards scenario development. Existing studies do not take into consideration the various social, technological, economic, environmental, political, legislative and ethical factors influencing the growth of the ocean economy. With the ocean increasingly being considered a new economic frontier, sustainable development has become pertinent for supporting and facilitating continued economic growth areas without compromising on environmental, social, or economic factors. Sustainable development within the ocean economy is further supported by Goal 14 of the Sustainable Development Goals, which focuses on conservation and sustainable use of the ocean, sea and marine resources. A literature review was conducted to gain a better understanding of the ocean economy, and primary data was collected through a real-time Delphi platform and semi-structured interviews. The real-time Delphi collected information on the key megatrends that continue to shape the ocean economy and key drivers and wild cards that can affect the sustainability of the key ocean economy industries. In addition, semi-structured interviews collected information on the proposed scenarios and the capacity needed in growing and promoting a sustainable ocean economy for South Africa towards 2060. A scenario matrix was developed for South Africa’s ocean economy and four scenarios and their implications were analysed. The preferred scenario was a resilient ocean economy towards 2060. The study also took a step forward with a view to provide reference to stakeholders and governments in progressing towards a possible scenario and developed an integrated vision for a sustainable ocean economy for South Africa towards 2060. This proposed vision is to ABSTRACT v mitigate against future challenges, collaborate and proactively utilise the development opportunities offered by the ocean economy. , Thesis (PhD) -- Faculty of Business and Economic science, 2022
- Full Text:
- Date Issued: 2022-04
Smart catchment management and application in the Swartkops River and Estuary
- Authors: Mmachaka,Thandi Nthabiseng
- Date: 2022-04
- Subjects: Estuarine ecology--South Africa--Swartkops River Estuary
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10948/55932 , vital:54401
- Description: The influence of urbanization and industrialization on natural resources results in complex water resource management. In South Africa, there has been escalating rural-urban migration, a growing population in urban areas, and the mushrooming of informal settlements in cities over the last five decades. In the pursuit of protecting water resources, these contextual realities create a significant challenge to policymakers, planners, and implementers. Due to rapid population growth, climate change, recent droughts, and growing competition among agricultural, industrial, commercial, environmental, and domestic sectors, water resources are presently under severe stress in South Africa. This situation necessitates the effective management of water resources. This study completed a situational assessment for the Swartkops Catchment to examine the extent of pollution, mapped sources of pollution, investigated water quality governance and identified hot spot areas to provide the foundation for effective and efficient catchment management. To determine the current water quality status of the Swartkops Catchment, this current study investigated spatial and temporal variation in physico-chemical parameters, nutrients, and faecal bacteria. This study applied a mixed-method research design using semi-structured interviews and focus groups discussions as data collection methods to investigate the water quality governance of the Swartkops Catchment. Following the situational assessment of the Swartkops Catchment, the effectiveness of applying innovative smart catchment practices to improve water quality was investigated. The word smart in this context refers to innovative mechanisms that will ensure effective and efficient water resources management. The PHP scripting language (Hypertext Preprocessor), Android studio, Wampserver, and JavaScript were used to develop the Water Use Screening System (WUSS) and Pollution Incident Reporting System (PRS). In South Africa, stormwater infrastructure suffers from unsustainable utilization. Stormwater systems discharge into many of the country‘s rivers and estuaries, including Swartkops, leading to water quality degradation. To improve the Recommended Ecological Category (REC) of the Swartkops Estuary to Category C, drivers that contribute to poor water quality must be understood to inform appropriate management interventions. Results from sampling in Markman Canal were compared with historical water quality data. The present study showed that the Markman Canal contributed faecal bacteria, nutrients, and trace metals to the Swartkops Estuary. The Markman Canal was the greatest source of trace metals to the Swartkops Estuary compared to the other point sources entering the estuary. The results show that arsenic and mercury levels in the Markman Canal and the Swartkops Estuary were higher than that previously measured. Iron and copper concentrations were higher in the Markman Canal whereas only copper increased in the Swartkops Estuary. , Thesis (PhD) -- Faculty of Science, School of Environmental Sciences, 2022
- Full Text:
- Date Issued: 2022-04
- Authors: Mmachaka,Thandi Nthabiseng
- Date: 2022-04
- Subjects: Estuarine ecology--South Africa--Swartkops River Estuary
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10948/55932 , vital:54401
- Description: The influence of urbanization and industrialization on natural resources results in complex water resource management. In South Africa, there has been escalating rural-urban migration, a growing population in urban areas, and the mushrooming of informal settlements in cities over the last five decades. In the pursuit of protecting water resources, these contextual realities create a significant challenge to policymakers, planners, and implementers. Due to rapid population growth, climate change, recent droughts, and growing competition among agricultural, industrial, commercial, environmental, and domestic sectors, water resources are presently under severe stress in South Africa. This situation necessitates the effective management of water resources. This study completed a situational assessment for the Swartkops Catchment to examine the extent of pollution, mapped sources of pollution, investigated water quality governance and identified hot spot areas to provide the foundation for effective and efficient catchment management. To determine the current water quality status of the Swartkops Catchment, this current study investigated spatial and temporal variation in physico-chemical parameters, nutrients, and faecal bacteria. This study applied a mixed-method research design using semi-structured interviews and focus groups discussions as data collection methods to investigate the water quality governance of the Swartkops Catchment. Following the situational assessment of the Swartkops Catchment, the effectiveness of applying innovative smart catchment practices to improve water quality was investigated. The word smart in this context refers to innovative mechanisms that will ensure effective and efficient water resources management. The PHP scripting language (Hypertext Preprocessor), Android studio, Wampserver, and JavaScript were used to develop the Water Use Screening System (WUSS) and Pollution Incident Reporting System (PRS). In South Africa, stormwater infrastructure suffers from unsustainable utilization. Stormwater systems discharge into many of the country‘s rivers and estuaries, including Swartkops, leading to water quality degradation. To improve the Recommended Ecological Category (REC) of the Swartkops Estuary to Category C, drivers that contribute to poor water quality must be understood to inform appropriate management interventions. Results from sampling in Markman Canal were compared with historical water quality data. The present study showed that the Markman Canal contributed faecal bacteria, nutrients, and trace metals to the Swartkops Estuary. The Markman Canal was the greatest source of trace metals to the Swartkops Estuary compared to the other point sources entering the estuary. The results show that arsenic and mercury levels in the Markman Canal and the Swartkops Estuary were higher than that previously measured. Iron and copper concentrations were higher in the Markman Canal whereas only copper increased in the Swartkops Estuary. , Thesis (PhD) -- Faculty of Science, School of Environmental Sciences, 2022
- Full Text:
- Date Issued: 2022-04
Synthesis and applications of novel fluorescent and colorimetric coumarin-based sensors towards analyte sensing in aqueous systems
- Authors: Battison, Aidan Leigh
- Date: 2022-04
- Subjects: Water-supply engineering--Technological innovations , Polymers--Optical properties , Fluorescent polymers
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10948/55954 , vital:54558
- Description: The continuous growth of mankind has not been considerate to the environment. The release of millions of tonnes of toxic heavy metal cations and anionic species through industrial, mining, agricultural, and electronic dumping has led to disease and, in many instances, death. This is usually suffered by low-income informal populations residing in third world countries. Moreover, many unnecessary deaths of children are becoming more prevalent because of consumption and contact with contaminated water, agricultural, and animal sources. Bioaccumulation of these toxic species in fish, plants, and animals, inevitably make their way back to the unaware general population. As growth by mining, agriculture, and electronics are indeed vital aspects of human development, the negative side effects of these activities usually continue unregulated. Therefore, as these processes are set to continue until more stringent regulatory processes are put into legislature; low-cost, sensitive, selective organic based sensors are a step in the right direction towards highlighting the need for environmental restoration and remediation; whilst also aiming to preventing unnecessary disease and death in the process. Herein, coumarin derived small-molecule fluorescent and colorimetric sensors for the quantitative and qualitative assessment of cationic and anionic species in aqueous and organic media are described. Ten fluorescent sensors supporting 1,4-disubstituted triazolyl moieties were synthesized according to Cu(I)-catalyzed azide-alkyne cycloaddition “click” reactions. These sensors were screened for their cationic and anionic affinities in a variety of solvent systems. Majority of the sensors responded well towards Fe3+, characterized by a strong fluorescent quenching response with a good degree of sensitivity and selectivity. Selected sensors were further investigated for their affinities towards anionic species; however, they did not display the same degree of selectivity or sensitivity towards these chosen anions. Titration studies of selected sensors with Fe3+ were able to be used towards determining the modes of fluorescent quenching; the photophysical mechanisms by which quenching occurs; stoichiometric binding ratios, association constants, and the number of coordination sites present between the sensors and Fe3+. Reversibility studies of the sensor-metal complex was investigated with EDTA. Partial reversibility was achieved for the chosen sensors with Fe3+. Hydrogen potential studies further described the application of these sensors over a good pH range. The binding site between the sensors and Fe3+ was investigated by NMR studies. , Thesis (PhD) -- Faculty of Science, School of Biomecular and Chemical Sciences, 2022
- Full Text:
- Date Issued: 2022-04
- Authors: Battison, Aidan Leigh
- Date: 2022-04
- Subjects: Water-supply engineering--Technological innovations , Polymers--Optical properties , Fluorescent polymers
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10948/55954 , vital:54558
- Description: The continuous growth of mankind has not been considerate to the environment. The release of millions of tonnes of toxic heavy metal cations and anionic species through industrial, mining, agricultural, and electronic dumping has led to disease and, in many instances, death. This is usually suffered by low-income informal populations residing in third world countries. Moreover, many unnecessary deaths of children are becoming more prevalent because of consumption and contact with contaminated water, agricultural, and animal sources. Bioaccumulation of these toxic species in fish, plants, and animals, inevitably make their way back to the unaware general population. As growth by mining, agriculture, and electronics are indeed vital aspects of human development, the negative side effects of these activities usually continue unregulated. Therefore, as these processes are set to continue until more stringent regulatory processes are put into legislature; low-cost, sensitive, selective organic based sensors are a step in the right direction towards highlighting the need for environmental restoration and remediation; whilst also aiming to preventing unnecessary disease and death in the process. Herein, coumarin derived small-molecule fluorescent and colorimetric sensors for the quantitative and qualitative assessment of cationic and anionic species in aqueous and organic media are described. Ten fluorescent sensors supporting 1,4-disubstituted triazolyl moieties were synthesized according to Cu(I)-catalyzed azide-alkyne cycloaddition “click” reactions. These sensors were screened for their cationic and anionic affinities in a variety of solvent systems. Majority of the sensors responded well towards Fe3+, characterized by a strong fluorescent quenching response with a good degree of sensitivity and selectivity. Selected sensors were further investigated for their affinities towards anionic species; however, they did not display the same degree of selectivity or sensitivity towards these chosen anions. Titration studies of selected sensors with Fe3+ were able to be used towards determining the modes of fluorescent quenching; the photophysical mechanisms by which quenching occurs; stoichiometric binding ratios, association constants, and the number of coordination sites present between the sensors and Fe3+. Reversibility studies of the sensor-metal complex was investigated with EDTA. Partial reversibility was achieved for the chosen sensors with Fe3+. Hydrogen potential studies further described the application of these sensors over a good pH range. The binding site between the sensors and Fe3+ was investigated by NMR studies. , Thesis (PhD) -- Faculty of Science, School of Biomecular and Chemical Sciences, 2022
- Full Text:
- Date Issued: 2022-04
The foraging ecology of female Cape fur seals (Arctocephalus pusillus pusillus) in South Africa
- Botha, Jonathan Aubrey, Kirkman, Stephen, Lombard, Amanda
- Authors: Botha, Jonathan Aubrey , Kirkman, Stephen , Lombard, Amanda
- Date: 2022-04
- Subjects: Seals (Animals)--Habitat—Conservation , Marine ecology—South Africa
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10948/55965 , vital:54559
- Description: The marine environment is spatially and temporally dynamic and many marine ecosystems are experiencing impacts of broad-scale climate change. Within these ecosystems, marine predators play a crucial role in ecosystem function through their top-down influences on prey populations. These predators may also respond to bottom-up influences and changes at lower trophic levels. A thorough understanding of marine predator foraging ecology is thus important to improve our understanding of ecosystem function, inform conservation and management measures, and to predict the response of predators to future environmental change. The Cape fur seal (Arctocephalus pusillus pusillus) comprises a major proportion of the southern African marine predator biomass. However, little is known about the foraging distribution and behaviour of the species, despite its obvious importance within the ecosystem and increasing concerns regarding seal-fisheries conflict. Using a variety of technologies, including, GPS and satellite-linked tags, dive behaviour recorders, accelerometers and stable isotope analysis, this thesis investigated the foraging ecology of female Cape fur seals breeding on the coast of South Africa. First, to identify important foraging areas and assess the influence of geographic, temporal and environmental factors on these areas, lactating females from three breeding colonies were fitted with GPS or Argos-linked tracking devices, while individuals from two of these colonies were also fitted with dive behaviour recorders. Results revealed geographic differences in the foraging distributions and ranges of individuals between breeding colonies, which likely reflects a combined influence of ecological setting and density dependant competition. In addition, dive behaviour recorders revealed the occurrence of benthic foraging at both sites where they were deployed, and the dominance of this strategy in some individuals. , Thesis (PhD) -- Faculty of Science, School of Environmental Sciences, 2022
- Full Text:
- Date Issued: 2022-04
- Authors: Botha, Jonathan Aubrey , Kirkman, Stephen , Lombard, Amanda
- Date: 2022-04
- Subjects: Seals (Animals)--Habitat—Conservation , Marine ecology—South Africa
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10948/55965 , vital:54559
- Description: The marine environment is spatially and temporally dynamic and many marine ecosystems are experiencing impacts of broad-scale climate change. Within these ecosystems, marine predators play a crucial role in ecosystem function through their top-down influences on prey populations. These predators may also respond to bottom-up influences and changes at lower trophic levels. A thorough understanding of marine predator foraging ecology is thus important to improve our understanding of ecosystem function, inform conservation and management measures, and to predict the response of predators to future environmental change. The Cape fur seal (Arctocephalus pusillus pusillus) comprises a major proportion of the southern African marine predator biomass. However, little is known about the foraging distribution and behaviour of the species, despite its obvious importance within the ecosystem and increasing concerns regarding seal-fisheries conflict. Using a variety of technologies, including, GPS and satellite-linked tags, dive behaviour recorders, accelerometers and stable isotope analysis, this thesis investigated the foraging ecology of female Cape fur seals breeding on the coast of South Africa. First, to identify important foraging areas and assess the influence of geographic, temporal and environmental factors on these areas, lactating females from three breeding colonies were fitted with GPS or Argos-linked tracking devices, while individuals from two of these colonies were also fitted with dive behaviour recorders. Results revealed geographic differences in the foraging distributions and ranges of individuals between breeding colonies, which likely reflects a combined influence of ecological setting and density dependant competition. In addition, dive behaviour recorders revealed the occurrence of benthic foraging at both sites where they were deployed, and the dominance of this strategy in some individuals. , Thesis (PhD) -- Faculty of Science, School of Environmental Sciences, 2022
- Full Text:
- Date Issued: 2022-04
The regulation of renewable energy in South Africa
- Van Huyssteen, Cornelis Roelof, Adelman, Sam
- Authors: Van Huyssteen, Cornelis Roelof , Adelman, Sam
- Date: 2022-04
- Subjects: International law and human rights , Climatic changes -- Social aspects --South Africa
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10948/56382 , vital:56617
- Description: As a developing country, South Africa’s demand for electricity is accelerating in order to facilitate economic growth and provide the necessary basic services to its people. Historically, the South African government has primarily opted to cater for the country’s electricity needs by means of coal as an affordable fossil fuel-based energy source. However, amidst the world’s changing climate and the plummeting costs of renewable energy technologies, there is increasing global pressure to phase out the use of fossil fuels as the key contributor to anthropogenic climate change and to move towards renewables as the world’s main source of energy. The primary problem highlighted in this thesis is the fact that South Africa’s energy laws have been developed to accommodate fossil fuels as the main source of energy, effectively neglecting the need to incorporate provisions that support and regulate the renewable energy sector. The thesis argues for the need for South Africa to transition away from fossil-fuel and nuclear based energy solutions based on the potential of renewables not only to improve energy security, but also to contribute towards social, economic, and environmental development. However, the transition to renewables and the realisation of its associated benefits will only be realised by means of a regulatory framework dedicated to the development of the renewable energy sector. The researcher argues that, in their current form, South African laws and policies limits the growth of the renewable energy sector. In order to propose reform and to develop the law to support renewable energy sufficiently, the thesis explores international law as well as global best practice in the United Kingdom and Germany related to renewable energy regulation. The comparative analysis allows the researcher to make recommendations to inform the development of South Africa’s renewable energy legal framework. It is argued that, given South Africa’s status as a developing country, such reform must be based upon the fundamental principles of climate change mitigation and social development. Ultimately, it is submitted that, a law dedicated to renewable energy can, and must, play an imperative role in realising the concept of sustainable development in South Africa. , Thesis (LLD) -- Faculty of Law , School of Public Law, 2022
- Full Text:
- Date Issued: 2022-04
- Authors: Van Huyssteen, Cornelis Roelof , Adelman, Sam
- Date: 2022-04
- Subjects: International law and human rights , Climatic changes -- Social aspects --South Africa
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10948/56382 , vital:56617
- Description: As a developing country, South Africa’s demand for electricity is accelerating in order to facilitate economic growth and provide the necessary basic services to its people. Historically, the South African government has primarily opted to cater for the country’s electricity needs by means of coal as an affordable fossil fuel-based energy source. However, amidst the world’s changing climate and the plummeting costs of renewable energy technologies, there is increasing global pressure to phase out the use of fossil fuels as the key contributor to anthropogenic climate change and to move towards renewables as the world’s main source of energy. The primary problem highlighted in this thesis is the fact that South Africa’s energy laws have been developed to accommodate fossil fuels as the main source of energy, effectively neglecting the need to incorporate provisions that support and regulate the renewable energy sector. The thesis argues for the need for South Africa to transition away from fossil-fuel and nuclear based energy solutions based on the potential of renewables not only to improve energy security, but also to contribute towards social, economic, and environmental development. However, the transition to renewables and the realisation of its associated benefits will only be realised by means of a regulatory framework dedicated to the development of the renewable energy sector. The researcher argues that, in their current form, South African laws and policies limits the growth of the renewable energy sector. In order to propose reform and to develop the law to support renewable energy sufficiently, the thesis explores international law as well as global best practice in the United Kingdom and Germany related to renewable energy regulation. The comparative analysis allows the researcher to make recommendations to inform the development of South Africa’s renewable energy legal framework. It is argued that, given South Africa’s status as a developing country, such reform must be based upon the fundamental principles of climate change mitigation and social development. Ultimately, it is submitted that, a law dedicated to renewable energy can, and must, play an imperative role in realising the concept of sustainable development in South Africa. , Thesis (LLD) -- Faculty of Law , School of Public Law, 2022
- Full Text:
- Date Issued: 2022-04
The use of psychometric test systems as a pre-selection tool for identifying successful harvesting machine operators
- Authors: Schwegman, Kylle
- Date: 2022-04
- Subjects: Port Elizabeth (South Africa) , Eastern Cape (South Africa) , South Africa
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10948/55246 , vital:51102
- Description: A shift towards fully mechanized forest activities, such as harvesting and, more recently, silviculture, has occurred from the early 2 000’s. The reason for mechanizing these activities differs for each country, although for most countries there is concern relating to the health and safety of forest workers. With mechanization in harvesting, the forest worker has moved away from the physical intensity of having to fell, debranch, debark and cross-cut trees into logs using either an axe or chainsaw, to operating a machine. Studies revolving around the human element (operator) have been conducted specifically relating to the ergonomics of the machines and how operators may influence machine productivity. Results showed that due to the repetitive nature of the work, the operators are exposed to associated stresses for longer periods. Typical injuries associated to these kinds of stressors are whole body vibrations (WBVs), repetitive hand and arm movements, non-neutral body postures and manual lifting, which lead to musculoskeletal symptoms in the lower back, neck and shoulders. However, as machine technology improves so does the ergonomic conditions which are experienced by machine operators. Machine productivity can be influenced by various factors, with the most influential being tree volume. However, a recent study showed that over 40% of variation in machine productivity has been observed amongst different machine operators operating similar machines. The specific reason for these differences has yet to be determined. However, decision making, motivation, planning capacity, concentration, memory, motor coordination, pattern recognition, logic reasoning, and spatial perception are abilities that have been described as important for successful harvesting work. The pre-selection of operators using psychometric and cognitive tests is not new to forestry, although very little information is available relating to the aptitude test known as the Vienna test system as well as the best possible demographic associated with successful harvesting operators. The overall aim of this study was to determine whether the high variation found amongst harvesting machine operators could be reduced initially through a preselection process which involves the use of aptitude tests (Vienna Test System) and demographic questionnaires. A study was implemented in Zululand, South Africa, testing whether there were significant productivity differences between nine machine operators. , Thesis (PhD) -- Faculty of Science, School of Natural Resource Management, 2022
- Full Text:
- Date Issued: 2022-04
- Authors: Schwegman, Kylle
- Date: 2022-04
- Subjects: Port Elizabeth (South Africa) , Eastern Cape (South Africa) , South Africa
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10948/55246 , vital:51102
- Description: A shift towards fully mechanized forest activities, such as harvesting and, more recently, silviculture, has occurred from the early 2 000’s. The reason for mechanizing these activities differs for each country, although for most countries there is concern relating to the health and safety of forest workers. With mechanization in harvesting, the forest worker has moved away from the physical intensity of having to fell, debranch, debark and cross-cut trees into logs using either an axe or chainsaw, to operating a machine. Studies revolving around the human element (operator) have been conducted specifically relating to the ergonomics of the machines and how operators may influence machine productivity. Results showed that due to the repetitive nature of the work, the operators are exposed to associated stresses for longer periods. Typical injuries associated to these kinds of stressors are whole body vibrations (WBVs), repetitive hand and arm movements, non-neutral body postures and manual lifting, which lead to musculoskeletal symptoms in the lower back, neck and shoulders. However, as machine technology improves so does the ergonomic conditions which are experienced by machine operators. Machine productivity can be influenced by various factors, with the most influential being tree volume. However, a recent study showed that over 40% of variation in machine productivity has been observed amongst different machine operators operating similar machines. The specific reason for these differences has yet to be determined. However, decision making, motivation, planning capacity, concentration, memory, motor coordination, pattern recognition, logic reasoning, and spatial perception are abilities that have been described as important for successful harvesting work. The pre-selection of operators using psychometric and cognitive tests is not new to forestry, although very little information is available relating to the aptitude test known as the Vienna test system as well as the best possible demographic associated with successful harvesting operators. The overall aim of this study was to determine whether the high variation found amongst harvesting machine operators could be reduced initially through a preselection process which involves the use of aptitude tests (Vienna Test System) and demographic questionnaires. A study was implemented in Zululand, South Africa, testing whether there were significant productivity differences between nine machine operators. , Thesis (PhD) -- Faculty of Science, School of Natural Resource Management, 2022
- Full Text:
- Date Issued: 2022-04
Zooplankton dynamics of Algoa Bay (South Africa) under varying oceanographic conditions
- Authors: Bizani,Mfundo
- Date: 2022-04
- Subjects: Marine zooplankton -- South Africa , Climate changes
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10948/55943 , vital:54554
- Description: the ecological significance of ocean plankton in terms of nutrient cycling and energy transfer is widely recognised, as is their mechanistic link with both oceanographic processes and climate. Consequently, plankton are widely regarded as sentinels of climate change, principally owing to their responsiveness to changing environmental conditions. Developing an understanding of the ecological as well as socio-economic consequences associated with changes in marine plankton dynamics has emerged as a key issue worldwide. Though many exciting advances in this respect have been attained with the aid of long-term plankton time series (>50 y), global environmental datasets and innovative meta-analyses, building deep knowledge of regional bio-physical dynamics and responses to specific perturbed states remains an essential component of the collective effort. Algoa Bay, situated on the warmtemperate south-east coast of South Africa presents a unique opportunity to study key elements of plankton ecology relevant to climate change problems in the regional sense. The embayment is locally subjected to frequent wind-driven upwelling and regionally, to periodic mesoscale oceanographic forcing (e.g., large solitary meanders, Agulhas Current intrusions), all of which culminate in a complex physical and biogeochemical environment. Using zooplankton as the focal component and drawing from a multi-year monthly dataset incorporating a bay-scale sampling framework, the present study addressed three key hypotheses pertaining to 1) drivers of spatio-temporal variability, 2) biomass rate of change over temporal scales using time-series analyses, and 3) community responses to a bay-scale biological perturbation event. Zooplankton biomass exhibited prominent seasonal patterns with bimodal peaks corresponding with near-consistent year-to-year autumn and spring blooms. Overall, the spatial trend showed no heterogeneity, though statistical significance between-station variability was noted per season. An analytical framework involving generalised additive models revealed zooplankton spatial heterogeneity in Algoa Bay to be a function of seasonal variation in a suite of physicochemical variables, including the combined effects of temperature, salinity, dissolved oxygen, total nitrogen and silicate, but not ammonium, phosphate or chlorophyll-a (as proxy for phytoplankton). Fronts (including river plumes), warm water intrusion from the Agulhas Current, and wind-driven upwelling were postulated to be key driving forces. Structural breaks analysis performed on station-specific time series revealed significant incremental increases in zooplankton biomass over time, which in turn, corresponded with a gradual decrease in thermocline depth. The most pronounced increasing effect was associated with a period (2013– 14) of amplified land-derived nutrient input and increasing harmful algal bloom (HAB) activity. , Thesis (PhD) -- Faculty of Science, School of Environmental Sciences, 2022
- Full Text:
- Date Issued: 2022-04
- Authors: Bizani,Mfundo
- Date: 2022-04
- Subjects: Marine zooplankton -- South Africa , Climate changes
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10948/55943 , vital:54554
- Description: the ecological significance of ocean plankton in terms of nutrient cycling and energy transfer is widely recognised, as is their mechanistic link with both oceanographic processes and climate. Consequently, plankton are widely regarded as sentinels of climate change, principally owing to their responsiveness to changing environmental conditions. Developing an understanding of the ecological as well as socio-economic consequences associated with changes in marine plankton dynamics has emerged as a key issue worldwide. Though many exciting advances in this respect have been attained with the aid of long-term plankton time series (>50 y), global environmental datasets and innovative meta-analyses, building deep knowledge of regional bio-physical dynamics and responses to specific perturbed states remains an essential component of the collective effort. Algoa Bay, situated on the warmtemperate south-east coast of South Africa presents a unique opportunity to study key elements of plankton ecology relevant to climate change problems in the regional sense. The embayment is locally subjected to frequent wind-driven upwelling and regionally, to periodic mesoscale oceanographic forcing (e.g., large solitary meanders, Agulhas Current intrusions), all of which culminate in a complex physical and biogeochemical environment. Using zooplankton as the focal component and drawing from a multi-year monthly dataset incorporating a bay-scale sampling framework, the present study addressed three key hypotheses pertaining to 1) drivers of spatio-temporal variability, 2) biomass rate of change over temporal scales using time-series analyses, and 3) community responses to a bay-scale biological perturbation event. Zooplankton biomass exhibited prominent seasonal patterns with bimodal peaks corresponding with near-consistent year-to-year autumn and spring blooms. Overall, the spatial trend showed no heterogeneity, though statistical significance between-station variability was noted per season. An analytical framework involving generalised additive models revealed zooplankton spatial heterogeneity in Algoa Bay to be a function of seasonal variation in a suite of physicochemical variables, including the combined effects of temperature, salinity, dissolved oxygen, total nitrogen and silicate, but not ammonium, phosphate or chlorophyll-a (as proxy for phytoplankton). Fronts (including river plumes), warm water intrusion from the Agulhas Current, and wind-driven upwelling were postulated to be key driving forces. Structural breaks analysis performed on station-specific time series revealed significant incremental increases in zooplankton biomass over time, which in turn, corresponded with a gradual decrease in thermocline depth. The most pronounced increasing effect was associated with a period (2013– 14) of amplified land-derived nutrient input and increasing harmful algal bloom (HAB) activity. , Thesis (PhD) -- Faculty of Science, School of Environmental Sciences, 2022
- Full Text:
- Date Issued: 2022-04
Accountability of the police to provincial governments in South Africa: a comparative analysis of law and practice in the eastern and Western Cape Provinces
- Authors: Tyabazayo, Phumlani
- Date: 2022-03
- Subjects: Police -- South Africa , Police power , Police administration
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/22456 , vital:52323
- Description: The 2012 Constitutional Court matter between the national minister of police and the premier of the Western Cape (Minister of Police v Premier of the Western Cape 2013 (12) BCLR 1365 (CC)) concerning the exercise of provincial policing powers as provided for in chapter 11 of the Constitution of South Africa laid bare the tension between the national and provincial governments in the exercise of these powers. Despite the Court’s determination of the dispute, there are still grey areas regarding the constitutional obligations of the provinces with regard to policing. The result has been a lack of clarity and uniformity in the exercise of policing powers by various provinces in South Africa. Based on documentary analysis and interviews, this study explores the parameters of the powers of provinces in policing matters as well as the extent of accountability of the police to provincial governments in South Africa by comparing the law and practice in the Eastern Cape and Western Cape provinces. The study argues that if provinces are to effectively exercise their constitutional powers in policing matters, there needs to be a degree of uniformity in and a mutual understanding of the extent and parameters of provincial policing powers. Only when they clearly understand their powers in policing matters will provincial governments be able to develop legal and policy frameworks to consolidate their powers and effectively hold the police accountable. The study considers whether the current policing powers afforded to provinces are adequate and if not, whether constitutional reform is needed to afford provinces more and clearer policing powers. Furthermore, within the context of participatory democracy, the study takes an in-depth look into the accountability role of Community Police Forums. The study finds that, generally, provincial governments fall short in fulfilling the obligations imposed on them by the Constitution. The study further finds that, in practice, there is still uncertainty about the role of the provincial governments and the Civilian Secretariat for Police in policing matters. Among the recommendations of this study is that section 206 (1) of the Constitution be amended to give provinces power to formulate policing policy in respect of issues peculiar to a particular province. The study also proposes a model to enhance the exercise of provincial policing powers by provincial governments in South Africa. , Thesis (PhD) -- Faculty of Law, 2022
- Full Text:
- Date Issued: 2022-03
- Authors: Tyabazayo, Phumlani
- Date: 2022-03
- Subjects: Police -- South Africa , Police power , Police administration
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/22456 , vital:52323
- Description: The 2012 Constitutional Court matter between the national minister of police and the premier of the Western Cape (Minister of Police v Premier of the Western Cape 2013 (12) BCLR 1365 (CC)) concerning the exercise of provincial policing powers as provided for in chapter 11 of the Constitution of South Africa laid bare the tension between the national and provincial governments in the exercise of these powers. Despite the Court’s determination of the dispute, there are still grey areas regarding the constitutional obligations of the provinces with regard to policing. The result has been a lack of clarity and uniformity in the exercise of policing powers by various provinces in South Africa. Based on documentary analysis and interviews, this study explores the parameters of the powers of provinces in policing matters as well as the extent of accountability of the police to provincial governments in South Africa by comparing the law and practice in the Eastern Cape and Western Cape provinces. The study argues that if provinces are to effectively exercise their constitutional powers in policing matters, there needs to be a degree of uniformity in and a mutual understanding of the extent and parameters of provincial policing powers. Only when they clearly understand their powers in policing matters will provincial governments be able to develop legal and policy frameworks to consolidate their powers and effectively hold the police accountable. The study considers whether the current policing powers afforded to provinces are adequate and if not, whether constitutional reform is needed to afford provinces more and clearer policing powers. Furthermore, within the context of participatory democracy, the study takes an in-depth look into the accountability role of Community Police Forums. The study finds that, generally, provincial governments fall short in fulfilling the obligations imposed on them by the Constitution. The study further finds that, in practice, there is still uncertainty about the role of the provincial governments and the Civilian Secretariat for Police in policing matters. Among the recommendations of this study is that section 206 (1) of the Constitution be amended to give provinces power to formulate policing policy in respect of issues peculiar to a particular province. The study also proposes a model to enhance the exercise of provincial policing powers by provincial governments in South Africa. , Thesis (PhD) -- Faculty of Law, 2022
- Full Text:
- Date Issued: 2022-03
An analysis of the internship programme in the Sarah Baartman Health District of the Eastern Cape: a model for effective implementation
- Authors: Feni, Monde Ludick
- Date: 2022-03
- Subjects: Internship programs , Public service employment , South Africa
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/23359 , vital:57617
- Description: The study’s main purpose was to evaluate the processes for Internship Programme Implementation in the Sarah Baartman Health District of the Eastern Cape Department of Health, to determine a Model that can be postulated for improving the Internship Programme Implementation in the Sarah Baartman Health District, wide Public Service and Private Sector. An empirical Study reveals the process for the Internship Programme Implementation has been marred with a significant number of challenges. As part of the recommendations, it was highly stressed that the Sarah Baartman Health District must adopt and embrace the Internship Programme Implementation Model in Figure 6.1 to effectively implement the Internship Programme as a means to deal with the challenges. Data was collected by the investigator from four main groups within the Sarah Baartman Health District. These were Senior Managers, Corporate Services Managers, Mentors, current and past interns. All these groups were directly involved in the internships in the Sarah Baartman Health District Health Department. The investigator got accurate and verified reflections, experiences and understandings of four constructs surrounding the internship programme and they include recruitment and employment of Interns using Best Practices, Training and Development Programmes for Interns Training, Performance Management and Development System for Interns and Mentoring as a Tool to Transfer Knowledge, Skills, Competencies and Attitudes required to Perform Optimally. During interviews, the researcher requested permission from the participants to record their responses digitally. This was done to make sure that no data was lost during the writing of responses from the participants. The questionnaires that were distributed to the interns and mentors had closed-ended questions, but the validity of the questionnaire was ensured by having a space for comments under each question to allow participants to support their answers. An analysis of the questionnaire was done by employing Microsoft Excel and statistics that are descriptive and inferential. The study findings revealed that the recruitment and employment of interns using best practices had a mean score above the required mean of 3, meaning that it is applied acceptably. As much as the construct is acceptable and meets the standard, the item in the construct that pertained to security checks for interns was not done and this is a worrying factor, meaning that the recruitment and employment of interns might be at risk of bringing in interns into the programme who are risky for the organisation. , Thesis (PhD) -- Faculty of Management and Commerce, 2022
- Full Text:
- Date Issued: 2022-03
- Authors: Feni, Monde Ludick
- Date: 2022-03
- Subjects: Internship programs , Public service employment , South Africa
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/23359 , vital:57617
- Description: The study’s main purpose was to evaluate the processes for Internship Programme Implementation in the Sarah Baartman Health District of the Eastern Cape Department of Health, to determine a Model that can be postulated for improving the Internship Programme Implementation in the Sarah Baartman Health District, wide Public Service and Private Sector. An empirical Study reveals the process for the Internship Programme Implementation has been marred with a significant number of challenges. As part of the recommendations, it was highly stressed that the Sarah Baartman Health District must adopt and embrace the Internship Programme Implementation Model in Figure 6.1 to effectively implement the Internship Programme as a means to deal with the challenges. Data was collected by the investigator from four main groups within the Sarah Baartman Health District. These were Senior Managers, Corporate Services Managers, Mentors, current and past interns. All these groups were directly involved in the internships in the Sarah Baartman Health District Health Department. The investigator got accurate and verified reflections, experiences and understandings of four constructs surrounding the internship programme and they include recruitment and employment of Interns using Best Practices, Training and Development Programmes for Interns Training, Performance Management and Development System for Interns and Mentoring as a Tool to Transfer Knowledge, Skills, Competencies and Attitudes required to Perform Optimally. During interviews, the researcher requested permission from the participants to record their responses digitally. This was done to make sure that no data was lost during the writing of responses from the participants. The questionnaires that were distributed to the interns and mentors had closed-ended questions, but the validity of the questionnaire was ensured by having a space for comments under each question to allow participants to support their answers. An analysis of the questionnaire was done by employing Microsoft Excel and statistics that are descriptive and inferential. The study findings revealed that the recruitment and employment of interns using best practices had a mean score above the required mean of 3, meaning that it is applied acceptably. As much as the construct is acceptable and meets the standard, the item in the construct that pertained to security checks for interns was not done and this is a worrying factor, meaning that the recruitment and employment of interns might be at risk of bringing in interns into the programme who are risky for the organisation. , Thesis (PhD) -- Faculty of Management and Commerce, 2022
- Full Text:
- Date Issued: 2022-03
Code of conduct for learners: A strategy for enhancing positive discipline in selected township schools in Durban, KwaZulu-Natal
- Zondo, Sindiswa Silindokuhle
- Authors: Zondo, Sindiswa Silindokuhle
- Date: 2022-03
- Subjects: School discipline
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/21711 , vital:51744
- Description: Dealing with misbehaving learners remains a significant challenge for teachers in South African schools. As the use of corporal punishment and other punitive measures to deal with misbehaving learners is now illegal, alternative positive disciplinary measures have been put in place. The prohibition of negative disciplinary measures has left teachers feeling powerless and frustrated, not knowing how to manage learner behaviour, despite the fact that they are the viable agents tasked with ensuring that positive disciplinary measures are implemented in schools. This study sought to examine how best a code of conduct for learners can be used to enhance positive discipline in schools. To that end, the study offers insights into how positive disciplinary measures may be used, in dealing with learner indiscipline. This is a qualitative study, underpinned by the interpretivist paradigm. The study employed a phenomenological design as a strategy for data collection, to examine how a code of conduct for learners can be used to enhance positive discipline in selected township schools. As this is a qualitative study concerned more with the depth than the breadth of information, the researcher deemed it necessary to focus on two schools, to obtain deep insight into the participants’ lived experiences. Two schools, in Pinetown in Durban, participated in the study. Interviews, observations and document reviews were used as data-collection instruments. In each school, interviews were conducted with the school principal, teacher representatives serving on the school governing board, disciplinary committee members, and members of the representative council for learners. Disciplinary hearings and disciplinary committee meetings were observed, and documents of relevance to the study were reviewed. The findings, as reported on here, revealed that the code of conduct is the most important strategy that schools can use to enhance positive discipline. Other strategies in enhancing positive discipline include the use of a research-based positive classroom management approach; positive reinforcement; integrating community support services; modelling good behaviour; parental involvement; preventive, supportive and corrective discipline; detention; communication (incorporating professionalism and cooperation); the creation of an environment conducive to teaching and learning; and the withdrawal or withholding of privileges. The findings further revealed that positive discipline is an alternative to negative disciplinary measures, such as corporal punishment in schools. Teachers are expected to comply with, and adhere to, disciplinary policies, and to assist learners in unlearning bad behaviours and developing the necessary life skills, values, and attitudes. The participants’ understanding of the use of a code of conduct for positive discipline successfully assisted them, as teachers, in dealing with misbehaving learners, and, in turn, in developing learners’ positive self-control and self-esteem, helping them to become responsible citizens, and courageous and respectful learners, not only in the school environment, but also as members of society at large. The research findings also revealed that many participants believed using a code of conduct (positive discipline) to enhance positive behaviour was a waste of time, and ineffective. As the findings indicate, learners were found to become impervious or immune to positive disciplinary measures put in place to assist them with behavioural problems. The findings also revealed that the ineffectiveness of such a code in enhancing positive discipline could be attributed to the fact that the teachers themselves were not, to a certain degree, fully trained with regard to positive disciplinary measures. A lack of parental support and teacher professionalism, the continued use of (the prohibited) corporal punishment, poor communication, a lack of stakeholder involvement in formulating codes of conduct, a lack of positive modelling and power struggles were found to impede the use of a code of conduct to enhance positive discipline in schools. Based on the research findings, the study recommends that schools ensure that there is a sufficiently detailed code of conduct to assist teachers in addressing learners’ bad behaviour. There should also be advocacy from the Department of Basic Education, for support programmes to be put in place to assist teachers with alternative ways of enhancing positive discipline in the classroom. Awareness of how teachers are to be upskilled as regards positive disciplinary strategies, active parental involvement, and increased support for teachers, are amongst the recommendations. Ultimately, the researcher concluded that the code of conduct was a strategy that schools can use to enhance positive discipline. To this end, teachers must adhere to that code (and other positive disciplinary measures), for positive discipline to flourish in schools. , Thesis (PhD) -- Faculty of Education, 2022
- Full Text:
- Date Issued: 2022-03
- Authors: Zondo, Sindiswa Silindokuhle
- Date: 2022-03
- Subjects: School discipline
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/21711 , vital:51744
- Description: Dealing with misbehaving learners remains a significant challenge for teachers in South African schools. As the use of corporal punishment and other punitive measures to deal with misbehaving learners is now illegal, alternative positive disciplinary measures have been put in place. The prohibition of negative disciplinary measures has left teachers feeling powerless and frustrated, not knowing how to manage learner behaviour, despite the fact that they are the viable agents tasked with ensuring that positive disciplinary measures are implemented in schools. This study sought to examine how best a code of conduct for learners can be used to enhance positive discipline in schools. To that end, the study offers insights into how positive disciplinary measures may be used, in dealing with learner indiscipline. This is a qualitative study, underpinned by the interpretivist paradigm. The study employed a phenomenological design as a strategy for data collection, to examine how a code of conduct for learners can be used to enhance positive discipline in selected township schools. As this is a qualitative study concerned more with the depth than the breadth of information, the researcher deemed it necessary to focus on two schools, to obtain deep insight into the participants’ lived experiences. Two schools, in Pinetown in Durban, participated in the study. Interviews, observations and document reviews were used as data-collection instruments. In each school, interviews were conducted with the school principal, teacher representatives serving on the school governing board, disciplinary committee members, and members of the representative council for learners. Disciplinary hearings and disciplinary committee meetings were observed, and documents of relevance to the study were reviewed. The findings, as reported on here, revealed that the code of conduct is the most important strategy that schools can use to enhance positive discipline. Other strategies in enhancing positive discipline include the use of a research-based positive classroom management approach; positive reinforcement; integrating community support services; modelling good behaviour; parental involvement; preventive, supportive and corrective discipline; detention; communication (incorporating professionalism and cooperation); the creation of an environment conducive to teaching and learning; and the withdrawal or withholding of privileges. The findings further revealed that positive discipline is an alternative to negative disciplinary measures, such as corporal punishment in schools. Teachers are expected to comply with, and adhere to, disciplinary policies, and to assist learners in unlearning bad behaviours and developing the necessary life skills, values, and attitudes. The participants’ understanding of the use of a code of conduct for positive discipline successfully assisted them, as teachers, in dealing with misbehaving learners, and, in turn, in developing learners’ positive self-control and self-esteem, helping them to become responsible citizens, and courageous and respectful learners, not only in the school environment, but also as members of society at large. The research findings also revealed that many participants believed using a code of conduct (positive discipline) to enhance positive behaviour was a waste of time, and ineffective. As the findings indicate, learners were found to become impervious or immune to positive disciplinary measures put in place to assist them with behavioural problems. The findings also revealed that the ineffectiveness of such a code in enhancing positive discipline could be attributed to the fact that the teachers themselves were not, to a certain degree, fully trained with regard to positive disciplinary measures. A lack of parental support and teacher professionalism, the continued use of (the prohibited) corporal punishment, poor communication, a lack of stakeholder involvement in formulating codes of conduct, a lack of positive modelling and power struggles were found to impede the use of a code of conduct to enhance positive discipline in schools. Based on the research findings, the study recommends that schools ensure that there is a sufficiently detailed code of conduct to assist teachers in addressing learners’ bad behaviour. There should also be advocacy from the Department of Basic Education, for support programmes to be put in place to assist teachers with alternative ways of enhancing positive discipline in the classroom. Awareness of how teachers are to be upskilled as regards positive disciplinary strategies, active parental involvement, and increased support for teachers, are amongst the recommendations. Ultimately, the researcher concluded that the code of conduct was a strategy that schools can use to enhance positive discipline. To this end, teachers must adhere to that code (and other positive disciplinary measures), for positive discipline to flourish in schools. , Thesis (PhD) -- Faculty of Education, 2022
- Full Text:
- Date Issued: 2022-03
Development of a strategy to promote prenatal physical activity participation among women in Buffalo City Municipality, South Africa
- Okafor, Uchenna Benedine https://orcid.org/0000-0002-0435-2041
- Authors: Okafor, Uchenna Benedine https://orcid.org/0000-0002-0435-2041
- Date: 2022-03
- Subjects: Prenatal care , Exercise for pregnant women
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/22919 , vital:53218
- Description: Research has proven that prenatal physical activity practice is associated with multiple health benefits; however, most women in South Africa rarely participate in prenatal physical activity. In contrast to more developed countries, there is dearth of research focusing specifically on prenatal physical activity and exercise among South African women. Nevertheless, in order to effectively, and strategically promote such prenatal activity, such information is important to plan for an intervention strategy. The main aim of this study was to assess the level, patterns, and associated factors of prenatal physical activity; beliefs, knowledge, attitudes, perceived benefits, and sources of information women received during pregnancy; and, furthermore, to develop an effective and relevant intervention strategy to facilitate the participation in this particular activity in Buffalo City Municipality, Eastern Cape, South Africa. Methods The study was a mixed-method, cross-sectional study design with both quantitative and qualitative data collection and analyses. The sequential explanatory design was adopted to merge and mix different datasets to be collected and analysed. The quantitative data involved a convenient sample of 1082 pregnant women in 12 randomly selected primary healthcare clinics offering antenatal health services in Buffalo City, Eastern Cape. The Pregnancy Physical Activity Questionnaire was interviewer-administered to women at each antenatal health clinic on pre-specified days, in a designated room allocated to the primary researcher by the health facility manager. The descriptive statistics were frequency distribution, percentages, mean and standard deviation. Furthermore, the bivariate and multivariate analyses were performed on two categories of participants, namely inactive or active, to determine the factors affecting prenatal physical activity behaviour. Furthermore, multiple logistic regression analyses were used to assess associations between physical activity and the predictor variables during pregnancy. Adjusted odds ratios with 95% confidence interval were applied to estimate factors associated with physical activity levels, and the associations between prenatal physical activity levels and socio-demographic, lifestyle, and obstetrics characteristics were determined using a chi-squared analyses. The significance level was set at p = 0.05. In addition, a qualitative descriptive approach was applied, using semi-structured face-to-face interviews with 15 purposively selected pregnant women, as well as 17 midwives offering antenatal health-care services to pregnant women in the 12 selected healthcare clinics. Qualitative data were analysed using a thematic content analysis. To develop the physical activity strategy for the promotion of prenatal physical activity practice, three frameworks were applied, namely the Strength, Weakness, Opportunity and Threat (SWOT), the Political, Economic Growth, Socio-Cultural, Technological, Laws and Environmental (PESTLE), and, lastly, the Build, Overcome, Explore and Minimise (BOEM) analytical frameworks. To facilitate the validation of the strategy, the findings were additionally analysed, after which, appropriate intervention strategies promoting prenatal physical activity were developed by again using the SWOT and PESTLE analytical strategic frameworks. This process involved a purposive sampling of seven experts with knowledge of and a proven academic and scholarly background in prenatal physical activity and maternal health. Next, the developed physical activity strategy was presented to various stakeholders, which included six primary healthcare managers, two midwives, and pregnant women purposively selected in each of the 12 chosen antenatal health clinics for the validation process. The stakeholders discussed, deliberated on, and provided comments and opinions of the feasibility and implementation of the developed prenatal physical activity for promotion of physical activity practices in the Eastern Cape Province. Results The findings of this particular study demonstrated low levels of prenatal physical activity among pregnant women, and, further indicated that the most preferred form of activity was light-intensity and household activities. Only 278 of the women (25.7%) met recommendations for prenatal activity (≥150 min moderate intensity exercise per week). The average time spent in moderate–vigorous physical activity was 151.6 min (95% CI: 147.2– 156.0). Most of the women participated in light exercises with a mean of 65.9% (95% CI: 64.8–67.0), and 47.6% (95% CI: 46.3–48.9) participated in household activities. The majority of the women did not receive physical activity advice during prenatal care sessions (64.7%). Lower age (<19 years) (adjusted odd ratio (AOR) = 0.3; CI: 0.16–0.76), semi-urban residence (AOR = 0.8; CI: 0.55–1.03), lower educational level (AOR = 0.5; CI: 0.20–0.71), unemployment (AOR = 0.5; CI: 0.29–0.77) and nulliparity (AOR = 0.6; CI: 0.28–1.31) were negatively associated with prenatal physical activity, while prenatal physical activity was positively associated with starting physical activity in the first trimester (AOR = 1.9; CI: 1.06–3.31) compared to other trimesters. In addition, the pregnant women were aware of the safety (88.2%) and benefits of physical activity for both mother and baby (79.6%), improved labour and delivery (93.1%), promote energy (89.0%), and should be discontinued when tired (76.6%). However, they also held the contradictory belief that pregnancy is “a time to rest” (56.5%). Furthermore, the most common sources of information about prenatal physical activity were the media, television, the radio and Internet-based websites (70.2%). Most women affirmed that prenatal physical activity reduced infant weight (61.4%), lessen moodiness (90.4), decreased risk of gestational diabetes mellitus (92.9%), pregnancy-induced hypertension (92.5%), and complications at birth (97.8%), while common negative perceptions included musculoskeletal discomfort (82.7%), and back pain (85.7%). The majority of women indicated that prenatal physical activity improved self-image (95.7%), sleep patterns (94.2%), and respiratory function (95.8%). The results from the quantitative data revealed that the major modifiable barriers to prenatal physical activity were tiredness (73.3%), lack of advice from healthcare professionals (nurses/midwives) (64.7%), low energy (64.5%), and non-accessibility to physical activity facilities (63.0%). The results were confirmed in the qualitative data, based on the Ecological Model, in which women also mentioned tiredness, work and household responsibilities, a lack of motivation, and the lack of physical activity advice and information on the relevant recommendations and guidelines. Overall, 62.4% women had high knowledge regarding prenatal physical activity; and half of the women showed a positive attitude toward it (50.1%). Whilst participants had knowledge of other types of antenatal exercises, 80.9% of the women had no knowledge of swimming exercise. Negative attitudes towards physical activity included the feeling of tiredness (67.7%), lack of interest (64.8%), and inadequate information on physical activity (59.5%). In addition, the study highlighted that midwives rarely educate and counsel pregnant women about prenatal physical activity during scheduled antenatal visits, which was attributed largely to the shortage of midwives handling many responsibilities at clinics. Nevertheless, the midwives did express a willingness to provide effective physical activity education and counselling on prenatal physical activity, if supported by relevant training and workshops. Furthermore, they further recommended the use of the Mom Connect application, which is a technological device designed by the National Department of Health, to distribute relevant information about maternal and child health. The prenatal physical activity strategies developed to address the above-mentioned and other barriers associated with prenatal physical activity include the use of scientific and technological innovations to provide basic information on prenatal physical activity to pregnant women by means of Mom Connect, and, by collaborating with the various cellphone and network companies in South Africa. Another strategy was to integrate prenatal physical activity training into the curricula of the existing higher institutions of learning that provide teaching of maternal health in the Eastern Cape Province. Additional strategies included the documentation and subsequent clarification of misconceptions about the safety concerns often associated with prenatal physical activity by making the documents accessible to all women at the clinics in the form of a small pamphlet or booklet. Lastly, stakeholders suggested that the government offer periodic prenatal physical activity campaigns, which should be presented in local community town halls and clinics and by other stakeholders to address the current lack of awareness and effectively eliminate misrepresentations and falsehoods around the safety of prenatal physical activity within geographical setting of the Eastern Cape Province. Conclusion Despite the advantages of prenatal physical activity practices, most pregnant women in South Africa do not participate in moderate-intensity physical activity. Notably, while women perceive prenatal physical activity as beneficial to both mother and baby, such theoretical knowledge is not easily translatable into practice. The predominant sources of information on prenatal physical activity are the television, the radio, and other media, which may be potentially misleading or contradictory to evidence-based physical activity practice. Furthermore, tiredness, a lack of time, work and household responsibilities, and a lack of motivation were major modifiable barriers to prenatal physical activity by the women. In addition, pregnant women rarely receive information on prenatal physical activity. Consequently, to address the needs of the pregnant women as highlighted in this study, a prenatal physical activity intervention strategy was developed and validated by key stakeholders to promote prenatal physical activity and exercise practice among women, taken in account the local context. , Thesis (PhD) -- Faculty of Health Sciences, 2022
- Full Text:
- Date Issued: 2022-03
- Authors: Okafor, Uchenna Benedine https://orcid.org/0000-0002-0435-2041
- Date: 2022-03
- Subjects: Prenatal care , Exercise for pregnant women
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/22919 , vital:53218
- Description: Research has proven that prenatal physical activity practice is associated with multiple health benefits; however, most women in South Africa rarely participate in prenatal physical activity. In contrast to more developed countries, there is dearth of research focusing specifically on prenatal physical activity and exercise among South African women. Nevertheless, in order to effectively, and strategically promote such prenatal activity, such information is important to plan for an intervention strategy. The main aim of this study was to assess the level, patterns, and associated factors of prenatal physical activity; beliefs, knowledge, attitudes, perceived benefits, and sources of information women received during pregnancy; and, furthermore, to develop an effective and relevant intervention strategy to facilitate the participation in this particular activity in Buffalo City Municipality, Eastern Cape, South Africa. Methods The study was a mixed-method, cross-sectional study design with both quantitative and qualitative data collection and analyses. The sequential explanatory design was adopted to merge and mix different datasets to be collected and analysed. The quantitative data involved a convenient sample of 1082 pregnant women in 12 randomly selected primary healthcare clinics offering antenatal health services in Buffalo City, Eastern Cape. The Pregnancy Physical Activity Questionnaire was interviewer-administered to women at each antenatal health clinic on pre-specified days, in a designated room allocated to the primary researcher by the health facility manager. The descriptive statistics were frequency distribution, percentages, mean and standard deviation. Furthermore, the bivariate and multivariate analyses were performed on two categories of participants, namely inactive or active, to determine the factors affecting prenatal physical activity behaviour. Furthermore, multiple logistic regression analyses were used to assess associations between physical activity and the predictor variables during pregnancy. Adjusted odds ratios with 95% confidence interval were applied to estimate factors associated with physical activity levels, and the associations between prenatal physical activity levels and socio-demographic, lifestyle, and obstetrics characteristics were determined using a chi-squared analyses. The significance level was set at p = 0.05. In addition, a qualitative descriptive approach was applied, using semi-structured face-to-face interviews with 15 purposively selected pregnant women, as well as 17 midwives offering antenatal health-care services to pregnant women in the 12 selected healthcare clinics. Qualitative data were analysed using a thematic content analysis. To develop the physical activity strategy for the promotion of prenatal physical activity practice, three frameworks were applied, namely the Strength, Weakness, Opportunity and Threat (SWOT), the Political, Economic Growth, Socio-Cultural, Technological, Laws and Environmental (PESTLE), and, lastly, the Build, Overcome, Explore and Minimise (BOEM) analytical frameworks. To facilitate the validation of the strategy, the findings were additionally analysed, after which, appropriate intervention strategies promoting prenatal physical activity were developed by again using the SWOT and PESTLE analytical strategic frameworks. This process involved a purposive sampling of seven experts with knowledge of and a proven academic and scholarly background in prenatal physical activity and maternal health. Next, the developed physical activity strategy was presented to various stakeholders, which included six primary healthcare managers, two midwives, and pregnant women purposively selected in each of the 12 chosen antenatal health clinics for the validation process. The stakeholders discussed, deliberated on, and provided comments and opinions of the feasibility and implementation of the developed prenatal physical activity for promotion of physical activity practices in the Eastern Cape Province. Results The findings of this particular study demonstrated low levels of prenatal physical activity among pregnant women, and, further indicated that the most preferred form of activity was light-intensity and household activities. Only 278 of the women (25.7%) met recommendations for prenatal activity (≥150 min moderate intensity exercise per week). The average time spent in moderate–vigorous physical activity was 151.6 min (95% CI: 147.2– 156.0). Most of the women participated in light exercises with a mean of 65.9% (95% CI: 64.8–67.0), and 47.6% (95% CI: 46.3–48.9) participated in household activities. The majority of the women did not receive physical activity advice during prenatal care sessions (64.7%). Lower age (<19 years) (adjusted odd ratio (AOR) = 0.3; CI: 0.16–0.76), semi-urban residence (AOR = 0.8; CI: 0.55–1.03), lower educational level (AOR = 0.5; CI: 0.20–0.71), unemployment (AOR = 0.5; CI: 0.29–0.77) and nulliparity (AOR = 0.6; CI: 0.28–1.31) were negatively associated with prenatal physical activity, while prenatal physical activity was positively associated with starting physical activity in the first trimester (AOR = 1.9; CI: 1.06–3.31) compared to other trimesters. In addition, the pregnant women were aware of the safety (88.2%) and benefits of physical activity for both mother and baby (79.6%), improved labour and delivery (93.1%), promote energy (89.0%), and should be discontinued when tired (76.6%). However, they also held the contradictory belief that pregnancy is “a time to rest” (56.5%). Furthermore, the most common sources of information about prenatal physical activity were the media, television, the radio and Internet-based websites (70.2%). Most women affirmed that prenatal physical activity reduced infant weight (61.4%), lessen moodiness (90.4), decreased risk of gestational diabetes mellitus (92.9%), pregnancy-induced hypertension (92.5%), and complications at birth (97.8%), while common negative perceptions included musculoskeletal discomfort (82.7%), and back pain (85.7%). The majority of women indicated that prenatal physical activity improved self-image (95.7%), sleep patterns (94.2%), and respiratory function (95.8%). The results from the quantitative data revealed that the major modifiable barriers to prenatal physical activity were tiredness (73.3%), lack of advice from healthcare professionals (nurses/midwives) (64.7%), low energy (64.5%), and non-accessibility to physical activity facilities (63.0%). The results were confirmed in the qualitative data, based on the Ecological Model, in which women also mentioned tiredness, work and household responsibilities, a lack of motivation, and the lack of physical activity advice and information on the relevant recommendations and guidelines. Overall, 62.4% women had high knowledge regarding prenatal physical activity; and half of the women showed a positive attitude toward it (50.1%). Whilst participants had knowledge of other types of antenatal exercises, 80.9% of the women had no knowledge of swimming exercise. Negative attitudes towards physical activity included the feeling of tiredness (67.7%), lack of interest (64.8%), and inadequate information on physical activity (59.5%). In addition, the study highlighted that midwives rarely educate and counsel pregnant women about prenatal physical activity during scheduled antenatal visits, which was attributed largely to the shortage of midwives handling many responsibilities at clinics. Nevertheless, the midwives did express a willingness to provide effective physical activity education and counselling on prenatal physical activity, if supported by relevant training and workshops. Furthermore, they further recommended the use of the Mom Connect application, which is a technological device designed by the National Department of Health, to distribute relevant information about maternal and child health. The prenatal physical activity strategies developed to address the above-mentioned and other barriers associated with prenatal physical activity include the use of scientific and technological innovations to provide basic information on prenatal physical activity to pregnant women by means of Mom Connect, and, by collaborating with the various cellphone and network companies in South Africa. Another strategy was to integrate prenatal physical activity training into the curricula of the existing higher institutions of learning that provide teaching of maternal health in the Eastern Cape Province. Additional strategies included the documentation and subsequent clarification of misconceptions about the safety concerns often associated with prenatal physical activity by making the documents accessible to all women at the clinics in the form of a small pamphlet or booklet. Lastly, stakeholders suggested that the government offer periodic prenatal physical activity campaigns, which should be presented in local community town halls and clinics and by other stakeholders to address the current lack of awareness and effectively eliminate misrepresentations and falsehoods around the safety of prenatal physical activity within geographical setting of the Eastern Cape Province. Conclusion Despite the advantages of prenatal physical activity practices, most pregnant women in South Africa do not participate in moderate-intensity physical activity. Notably, while women perceive prenatal physical activity as beneficial to both mother and baby, such theoretical knowledge is not easily translatable into practice. The predominant sources of information on prenatal physical activity are the television, the radio, and other media, which may be potentially misleading or contradictory to evidence-based physical activity practice. Furthermore, tiredness, a lack of time, work and household responsibilities, and a lack of motivation were major modifiable barriers to prenatal physical activity by the women. In addition, pregnant women rarely receive information on prenatal physical activity. Consequently, to address the needs of the pregnant women as highlighted in this study, a prenatal physical activity intervention strategy was developed and validated by key stakeholders to promote prenatal physical activity and exercise practice among women, taken in account the local context. , Thesis (PhD) -- Faculty of Health Sciences, 2022
- Full Text:
- Date Issued: 2022-03
Examining the realisation of the Multisectoral Early Childhood Development Policy short-term goals in the Eastern Cape Province, South Africa
- Kula, Nonkqubela Carvie https://orcid.org/0000-0002-0770-919X
- Authors: Kula, Nonkqubela Carvie https://orcid.org/0000-0002-0770-919X
- Date: 2022-03
- Subjects: Early childhood education -- South Africa , Child development , Education, Preschool
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/21646 , vital:51739
- Description: This study was triggered by global concerns about poor early childhood development (ECD) policy implementation. A similar situation existed in South Africa in respect of the implementation of the National Integrated Early Childhood Development (NIECD) policy. This study was undertaken to examine the realisation of the short-term goals of the NIECD policy in the Eastern Cape province, South Africa. Through this study, the researcher hoped to propose an appropriate framework that can be adopted by the Eastern Cape provincial government to realise the short-term goals. A qualitative study was undertaken using the purposive sampling method to identify managers who are knowledgeable on ECD in the three lead departments (DSD, DOH, DBE) at provincial, district, and local levels. Twelve participants (N=12) were identified: three ECD directors from the provincial departments, three district ECD managers from each municipal district, namely, OR Tambo, Sarah Baartman and Buffalo City. Semi-structured interviews based on the study objectives were carried out on the ECD directors from the provincial departments. Three focus group interviews were carried out on the district ECD managers from each municipal district, also based on the study objectives. Each interview was transcribed verbatim by the researcher. Interviewees were coded to ensure confidentiality. The four study objectives were used as themes for data analysis. The findings of the study showed a poor understanding of the NIECD policy at the district level and a lack of a provincial multisectoral implementation strategy. Staff shortage, lack of multisectoral ECD coordination, fragmentation of ECD services, high numbers of unregistered ECD centres and inadequate ECD funding were the main barriers to policy implementation. The study recommends that the Eastern Cape provincial government uses systems theory to develop its provincial ECD implementation strategy. The framework of the ECD strategy should focus on the inputs, processes, outputs, and feedback process map. In this regard, inputs include human resources, infrastructure, funding, leadership and governance, data management, systems technology, and service delivery. Outputs, on the other hand, comprise service delivery (universally accessible ECD services, universal coverage of ECD services, parent participation, multisectoral ECD services and quality ECD services). Feedback indicates whether all infants, young children and their families are living in environments that are conducive to their optimal development or not. Should the implementation of the policy fail, corrections need to be made in the input or process stage or both stages. , Thesis (PhD) -- Faculty of Education, 2022
- Full Text:
- Date Issued: 2022-03
- Authors: Kula, Nonkqubela Carvie https://orcid.org/0000-0002-0770-919X
- Date: 2022-03
- Subjects: Early childhood education -- South Africa , Child development , Education, Preschool
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/21646 , vital:51739
- Description: This study was triggered by global concerns about poor early childhood development (ECD) policy implementation. A similar situation existed in South Africa in respect of the implementation of the National Integrated Early Childhood Development (NIECD) policy. This study was undertaken to examine the realisation of the short-term goals of the NIECD policy in the Eastern Cape province, South Africa. Through this study, the researcher hoped to propose an appropriate framework that can be adopted by the Eastern Cape provincial government to realise the short-term goals. A qualitative study was undertaken using the purposive sampling method to identify managers who are knowledgeable on ECD in the three lead departments (DSD, DOH, DBE) at provincial, district, and local levels. Twelve participants (N=12) were identified: three ECD directors from the provincial departments, three district ECD managers from each municipal district, namely, OR Tambo, Sarah Baartman and Buffalo City. Semi-structured interviews based on the study objectives were carried out on the ECD directors from the provincial departments. Three focus group interviews were carried out on the district ECD managers from each municipal district, also based on the study objectives. Each interview was transcribed verbatim by the researcher. Interviewees were coded to ensure confidentiality. The four study objectives were used as themes for data analysis. The findings of the study showed a poor understanding of the NIECD policy at the district level and a lack of a provincial multisectoral implementation strategy. Staff shortage, lack of multisectoral ECD coordination, fragmentation of ECD services, high numbers of unregistered ECD centres and inadequate ECD funding were the main barriers to policy implementation. The study recommends that the Eastern Cape provincial government uses systems theory to develop its provincial ECD implementation strategy. The framework of the ECD strategy should focus on the inputs, processes, outputs, and feedback process map. In this regard, inputs include human resources, infrastructure, funding, leadership and governance, data management, systems technology, and service delivery. Outputs, on the other hand, comprise service delivery (universally accessible ECD services, universal coverage of ECD services, parent participation, multisectoral ECD services and quality ECD services). Feedback indicates whether all infants, young children and their families are living in environments that are conducive to their optimal development or not. Should the implementation of the policy fail, corrections need to be made in the input or process stage or both stages. , Thesis (PhD) -- Faculty of Education, 2022
- Full Text:
- Date Issued: 2022-03
Financing tools, firm life cycle and growth of small, medium and micro enterprises in selected sub-Sahara African economies
- Ngonisa, Phillip https://orcid.org/0000-0002-0145-9062
- Authors: Ngonisa, Phillip https://orcid.org/0000-0002-0145-9062
- Date: 2022-03
- Subjects: Small business -- Finance , Small business -- Africa
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/21794 , vital:51754
- Description: Finance is of paramount importance for small, medium and micro enterprises’ (SMMEs) growth, without which many firms fail to move along their growth continuum. Against this background, the study sought to examine the growth effects of financing tools across the different phases of SMMEs' life cycle in Sub Sahara Africa (SSA) economies for the period spanning from 2003 to 2019. Firstly, due to the inherent heterogeneity nature of the SMMEs' financing landscapes across the world, the study starts by identifying the commonly used financing tools in the context of Sub-Saharan Africa. These were found to be internal finance, bank debt, trade credit, non-bank finance and informal finance, with internal finance being the most prevalent financing tool. The second aspect of the study was to examine the growth effects of the identified financing tools on SMMEs' performance in Sub Sahara Africa. In achieving this objective, several panel estimation techniques were employed, which are the feasible generalised least squares (FGLS), cross-sectional dependence (SCC), random effects model (REM) and pooled ordinary least squares (POLS). The empirical results show that internal finance or savings, bank financing, trade credit, non-bank financing and informal financing are positive and statistically significant in explaining SMMEs growth in the region, with stronger evidence for a positive relationship between external finance (trade credit and bank finance) on SMMEs growth in Sub-Saharan African region. The third objective of the study was to investigate the growth effects of SMMEs’ financing tools across different phases of the firm life cycle. The same panel techniques as used in achieving the previous objective were utilized again. The empirical findings show that the growth effects of SMME financing tools evolve as SMMEs move along their growth continuum, and only bank finance proved to be a fundamental variable for SMMEs growth throughout the different phases of firm growth. Finally, motivated by SMMEs’ high dependence on internal finance or savings, the study explored the saving practices of SMMEs in Sub Saharan Africa using thematic analysis. The study findings show that SMMEs in Sub Sahara African economies systematically save through formal and informal financial systems. These findings are contrary to conventional wisdom, which suggests that SMMEs are a financial basket case. Basing on the study findings, policies aimed at reducing or lessening the burden of accessing finance are important to stimulate the growth of SMMEs. Most importantly, there is a need for lenders and sponsors to understand the firm life cycle-financing tool nexus to ensure SMMEs growth. Moreover, SMMEs in Sub Sahara African economies need to cultivate a spirit of thrift to minimize firm attrition rate, thereby promoting SMMEs' growth in the region. , Thesis (PhD) -- Faculty of Management and Commerce, 2022
- Full Text:
- Date Issued: 2022-03
- Authors: Ngonisa, Phillip https://orcid.org/0000-0002-0145-9062
- Date: 2022-03
- Subjects: Small business -- Finance , Small business -- Africa
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/21794 , vital:51754
- Description: Finance is of paramount importance for small, medium and micro enterprises’ (SMMEs) growth, without which many firms fail to move along their growth continuum. Against this background, the study sought to examine the growth effects of financing tools across the different phases of SMMEs' life cycle in Sub Sahara Africa (SSA) economies for the period spanning from 2003 to 2019. Firstly, due to the inherent heterogeneity nature of the SMMEs' financing landscapes across the world, the study starts by identifying the commonly used financing tools in the context of Sub-Saharan Africa. These were found to be internal finance, bank debt, trade credit, non-bank finance and informal finance, with internal finance being the most prevalent financing tool. The second aspect of the study was to examine the growth effects of the identified financing tools on SMMEs' performance in Sub Sahara Africa. In achieving this objective, several panel estimation techniques were employed, which are the feasible generalised least squares (FGLS), cross-sectional dependence (SCC), random effects model (REM) and pooled ordinary least squares (POLS). The empirical results show that internal finance or savings, bank financing, trade credit, non-bank financing and informal financing are positive and statistically significant in explaining SMMEs growth in the region, with stronger evidence for a positive relationship between external finance (trade credit and bank finance) on SMMEs growth in Sub-Saharan African region. The third objective of the study was to investigate the growth effects of SMMEs’ financing tools across different phases of the firm life cycle. The same panel techniques as used in achieving the previous objective were utilized again. The empirical findings show that the growth effects of SMME financing tools evolve as SMMEs move along their growth continuum, and only bank finance proved to be a fundamental variable for SMMEs growth throughout the different phases of firm growth. Finally, motivated by SMMEs’ high dependence on internal finance or savings, the study explored the saving practices of SMMEs in Sub Saharan Africa using thematic analysis. The study findings show that SMMEs in Sub Sahara African economies systematically save through formal and informal financial systems. These findings are contrary to conventional wisdom, which suggests that SMMEs are a financial basket case. Basing on the study findings, policies aimed at reducing or lessening the burden of accessing finance are important to stimulate the growth of SMMEs. Most importantly, there is a need for lenders and sponsors to understand the firm life cycle-financing tool nexus to ensure SMMEs growth. Moreover, SMMEs in Sub Sahara African economies need to cultivate a spirit of thrift to minimize firm attrition rate, thereby promoting SMMEs' growth in the region. , Thesis (PhD) -- Faculty of Management and Commerce, 2022
- Full Text:
- Date Issued: 2022-03
Threatened plant species in Vhembe Biosphere Reserve, Limpopo province, South Africa: Problems and prospects of conservation and utilization
- Ramarumo, Luambo Jeffrey https://orcid.org/0000-0002-2393-6982
- Authors: Ramarumo, Luambo Jeffrey https://orcid.org/0000-0002-2393-6982
- Date: 2022-03
- Subjects: Plants -- Extinction , Rare plants , Botany, Economic
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/22754 , vital:52738
- Description: Threatened plant species are those species that are vulnerable or at the risk of extinction. According to Version 3.1 of the International Union for Conservation of Nature (IUCN)’s Red List Categories and Criteria, the three categories of threat in order of increasing risk of extinction are: Vulnerable (VU), Endangered (EN) and Critically Endangered (CR). These species are oftenly protected by both national and international obligations. Scientific evidence suggests that threatened plants are disappearing at an alarming rate. The current expansion of agricultural land, urbanization, over-exploitation of biological resources, climate change and invasive alien species are regarded as major drivers of biodiversity loss and high rate of species extinction worldwide. Scientists and conservation managers are seeking to understand and monitor plant species that are likely to be on the verge of extinction. Monitoring of threatened plants can be better achieved through insights about indigenous knowledge dynamics associated with such species. Scientific scholars including botanists, ethnobotanists, conservationists and anthropologists, all share common interest about the use of indigenous knowledge for livelihoods, scientific and economic growth. As a result, there is a growing interest on indigenous knowledge researches, particularly involving utilization and conservation of plant species. Given the fact that recent scientific evidence suggests that such studies are lacking in South African Biosphere Reserves, as well as the fact that scientist and conservation managers are seeking to understand species likely to be on the verge of extinction risk. It is within this context that this study is aimed at investigating threatened plant species utilization, conservation statuses and distribution in the Vhembe Biosphere Reserve, Limpopo Province in South Africa. The hypothesis of this study states that traditional knowledge about utilization, distribution and conservation statuses of threatened plant species provide suggestions for appropriate conservation practices. Since the current study is ethnobotanical in nature that is focusing on human interactions with plants, the research methods used addressed multidisciplinary aspects and involved disciplinary integration. An integrated participatory research approach focusing on shared learning, forging collaborative relations with participants, analyzing and validating the shared knowledge was used to document ethnobotanical data within the study area. This research approach was selected as it is considered to be a quick and effective way of acquiring data associated with indigenous knowledge systems. To offset the elements of bias during data collection, the research technique was designed to accommodate core principles that interlinked participatory rural appraisal (PRA) and rapid rural appraisal (RRA). Furthermore, the integrated core principles of participatory rural appraisal and rapid rural appraisal were used with conventional methods such as field surveys and interviews using questionnaires. Therefore, ethnobotanical data were collected through interviews with 203 participants. The data associated with threatened plant’s population size were gathered through literal counting of individual plants as per the IUCN’s Red List Criteria. Data associated with the conservation statuses were gathered from both the South African National Biodiversity Institute and IUCN databases. A total of 13 useful threatened plants belonging to 12 families were recorded with their conservation statuses ranging from being Vulnerable to Critically Endangered. The majority of the threatened plant species (46.0percent) were used for medicinal purposes only, followed by species used for medicinal purposes and as ornamentals (23.0percent). The frequently cited useful threatened species with use values (UV) > 0.024, relative frequency citation (RFC) > 0.059 and fidelity level percentage (FLpercent) > 5.911percent, included Asparagus sekukuniensis, Bowiea volubilis, Brackenridgea zanguebarica, Ocotea bullata, Rhynchosia vendae, Siphonochilus aethiopicus and Warburgia salutaris. About 47.0percent of the recorded useful threatened plants were distributed in remote areas of the Thathe Vonḓo and its surroundings. Threatened plants with population size < 100 adult individuals constituted the majority (61.54percent). Birdlime-making plant species were also documented in the current study. A total of 12 birdlime-making plants belonging to six families were recorded, including threatened Huernia nouhuysii, which is categorized as Vulnerable in South Africa. Amongst the recorded families, Loranthaceae and Euphorbiaceae were categorized as the most frequently utilized families. Among the recorded species, six of them were reported to being used in the birdlime-making for the first time and these species include Euphorbia pulvinata (17.2percent), followed by Tapinanthus forbesii (8percent), Tapinanthus rubromarginatus (7.2percent), Erianthemum ngamicum (7.2percent), Englerophytum magalismon-tanum (3.6percent), Huernia nouhuysii (2.0percent), and Euphorbia tirucalli (0.8percent). Only three plant parts were utilized for birdlime-making. The milky latex was preferred plant part (58.3percent), followed by fruit (33.3percent) and root bark (8.4percent). Birdlime-making techniques involved crushing, which accounted for 55.7percent, followed by air blown (29.6percent) and boiling (14.7percent). Furthermore, the complementary contribution of birdlime toward human development included, being used for bird hunting or trapping small birds (45.8percent), adhesion (23.2percent), teeth cleaning (17.2percent) and roof-waterproofing (13.8percent). The indigenous conservation strategies employed by participants included harvesting of single lateral root per individual medicinal plant (15.3percent), medicinal and timber materials are only harvested during winter season (16.7percent), the use of moist soil to cover injured plant part after bark harvest (18.2percent), prohibit harvesting of and/ or from an injured plant (11.8percent), collection of dried or fallen plants for firewood (8.4percent), issuing of permits by traditional council through the chief or headman to allow collection of medicinal materials and timber (9.9percent), prohibit chopping down of medicinal plant species (13.8percent) and collection of some Critically Endangered plant species such as Brackenridgea zanguebarica and Siphonochilus aethiopicus during the night by authorized people only (5.9percent). The hypothesis which stated that traditional knowledge about utilization, distribution and conservation statuses of threatened plant species provide suggestions for appropriate conservation practices can therefore, not be rejected as there is room for further and more detailed ethnobotanical investigations that is focused on human interactions with threatened plant species. The data presented in this thesis could be used as baseline information for formulating new conservation strategies, monitoring and management plans of threatened plant species not only in the Vhembe Biosphere Reserve, but in other regions of South African. This study provided insights associated with ethnomedicinal uses of Asparagus sekukuniensis, Protea laetans and Encephalartos hirsutus. Results of this study could also stimulate interest in other scientific disciplines such as the phytochemistry, pharmacology, bioprocessing, conservation and anthropology involving documentation threatened plant species. , Thesis (PhD) -- Faculty of Science and Agriculture, 2022
- Full Text:
- Date Issued: 2022-03
- Authors: Ramarumo, Luambo Jeffrey https://orcid.org/0000-0002-2393-6982
- Date: 2022-03
- Subjects: Plants -- Extinction , Rare plants , Botany, Economic
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/22754 , vital:52738
- Description: Threatened plant species are those species that are vulnerable or at the risk of extinction. According to Version 3.1 of the International Union for Conservation of Nature (IUCN)’s Red List Categories and Criteria, the three categories of threat in order of increasing risk of extinction are: Vulnerable (VU), Endangered (EN) and Critically Endangered (CR). These species are oftenly protected by both national and international obligations. Scientific evidence suggests that threatened plants are disappearing at an alarming rate. The current expansion of agricultural land, urbanization, over-exploitation of biological resources, climate change and invasive alien species are regarded as major drivers of biodiversity loss and high rate of species extinction worldwide. Scientists and conservation managers are seeking to understand and monitor plant species that are likely to be on the verge of extinction. Monitoring of threatened plants can be better achieved through insights about indigenous knowledge dynamics associated with such species. Scientific scholars including botanists, ethnobotanists, conservationists and anthropologists, all share common interest about the use of indigenous knowledge for livelihoods, scientific and economic growth. As a result, there is a growing interest on indigenous knowledge researches, particularly involving utilization and conservation of plant species. Given the fact that recent scientific evidence suggests that such studies are lacking in South African Biosphere Reserves, as well as the fact that scientist and conservation managers are seeking to understand species likely to be on the verge of extinction risk. It is within this context that this study is aimed at investigating threatened plant species utilization, conservation statuses and distribution in the Vhembe Biosphere Reserve, Limpopo Province in South Africa. The hypothesis of this study states that traditional knowledge about utilization, distribution and conservation statuses of threatened plant species provide suggestions for appropriate conservation practices. Since the current study is ethnobotanical in nature that is focusing on human interactions with plants, the research methods used addressed multidisciplinary aspects and involved disciplinary integration. An integrated participatory research approach focusing on shared learning, forging collaborative relations with participants, analyzing and validating the shared knowledge was used to document ethnobotanical data within the study area. This research approach was selected as it is considered to be a quick and effective way of acquiring data associated with indigenous knowledge systems. To offset the elements of bias during data collection, the research technique was designed to accommodate core principles that interlinked participatory rural appraisal (PRA) and rapid rural appraisal (RRA). Furthermore, the integrated core principles of participatory rural appraisal and rapid rural appraisal were used with conventional methods such as field surveys and interviews using questionnaires. Therefore, ethnobotanical data were collected through interviews with 203 participants. The data associated with threatened plant’s population size were gathered through literal counting of individual plants as per the IUCN’s Red List Criteria. Data associated with the conservation statuses were gathered from both the South African National Biodiversity Institute and IUCN databases. A total of 13 useful threatened plants belonging to 12 families were recorded with their conservation statuses ranging from being Vulnerable to Critically Endangered. The majority of the threatened plant species (46.0percent) were used for medicinal purposes only, followed by species used for medicinal purposes and as ornamentals (23.0percent). The frequently cited useful threatened species with use values (UV) > 0.024, relative frequency citation (RFC) > 0.059 and fidelity level percentage (FLpercent) > 5.911percent, included Asparagus sekukuniensis, Bowiea volubilis, Brackenridgea zanguebarica, Ocotea bullata, Rhynchosia vendae, Siphonochilus aethiopicus and Warburgia salutaris. About 47.0percent of the recorded useful threatened plants were distributed in remote areas of the Thathe Vonḓo and its surroundings. Threatened plants with population size < 100 adult individuals constituted the majority (61.54percent). Birdlime-making plant species were also documented in the current study. A total of 12 birdlime-making plants belonging to six families were recorded, including threatened Huernia nouhuysii, which is categorized as Vulnerable in South Africa. Amongst the recorded families, Loranthaceae and Euphorbiaceae were categorized as the most frequently utilized families. Among the recorded species, six of them were reported to being used in the birdlime-making for the first time and these species include Euphorbia pulvinata (17.2percent), followed by Tapinanthus forbesii (8percent), Tapinanthus rubromarginatus (7.2percent), Erianthemum ngamicum (7.2percent), Englerophytum magalismon-tanum (3.6percent), Huernia nouhuysii (2.0percent), and Euphorbia tirucalli (0.8percent). Only three plant parts were utilized for birdlime-making. The milky latex was preferred plant part (58.3percent), followed by fruit (33.3percent) and root bark (8.4percent). Birdlime-making techniques involved crushing, which accounted for 55.7percent, followed by air blown (29.6percent) and boiling (14.7percent). Furthermore, the complementary contribution of birdlime toward human development included, being used for bird hunting or trapping small birds (45.8percent), adhesion (23.2percent), teeth cleaning (17.2percent) and roof-waterproofing (13.8percent). The indigenous conservation strategies employed by participants included harvesting of single lateral root per individual medicinal plant (15.3percent), medicinal and timber materials are only harvested during winter season (16.7percent), the use of moist soil to cover injured plant part after bark harvest (18.2percent), prohibit harvesting of and/ or from an injured plant (11.8percent), collection of dried or fallen plants for firewood (8.4percent), issuing of permits by traditional council through the chief or headman to allow collection of medicinal materials and timber (9.9percent), prohibit chopping down of medicinal plant species (13.8percent) and collection of some Critically Endangered plant species such as Brackenridgea zanguebarica and Siphonochilus aethiopicus during the night by authorized people only (5.9percent). The hypothesis which stated that traditional knowledge about utilization, distribution and conservation statuses of threatened plant species provide suggestions for appropriate conservation practices can therefore, not be rejected as there is room for further and more detailed ethnobotanical investigations that is focused on human interactions with threatened plant species. The data presented in this thesis could be used as baseline information for formulating new conservation strategies, monitoring and management plans of threatened plant species not only in the Vhembe Biosphere Reserve, but in other regions of South African. This study provided insights associated with ethnomedicinal uses of Asparagus sekukuniensis, Protea laetans and Encephalartos hirsutus. Results of this study could also stimulate interest in other scientific disciplines such as the phytochemistry, pharmacology, bioprocessing, conservation and anthropology involving documentation threatened plant species. , Thesis (PhD) -- Faculty of Science and Agriculture, 2022
- Full Text:
- Date Issued: 2022-03
A framework for communicating climate information to rural small-scale farmers in the Eastern Cape Province, South Africa using systems thinking approach
- Pindura, Tineyi Herbert https://orcid.org/0000-0001-7233-6222
- Authors: Pindura, Tineyi Herbert https://orcid.org/0000-0001-7233-6222
- Date: 2022-02
- Subjects: Farms, Small , Agriculture -- Environmental aspects , Climatic changes
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/22809 , vital:52784
- Description: In the Eastern Cape of South Africa, rural small-scale farmers live in uncertain times characterized by climate change and variability, which intensify social, political and financial inequalities. Therefore, there is a need to increase the understanding and interpretation of climate information to minimize crop production risk, reduce rural small-scale farmers’ vulnerability to climate, and maximize opportunities. Increasing the resilience among rural small-scale farmers requires appropriate and viable practical approaches. By using systems thinking approach (and the Raymond Mhlaba Municipality in the Eastern Cape as a study area), this research disseminates the complex nature of current climate information frameworks. Through inputs from farmers (through a Farmers Research Group methodology) and climate data, this thesis developed a new framework for communicating climate information (herein referred as the climate information communication systems framework) to rural small-scale farmers. The proposed climate information communication systems framework successfully integrates scientific and traditional knowledge. The framework constitutes certain stages, where the farming system and crop identification is the first stage. The second stage is the requirements stage, which has two relationships: scientist to crop requirements and farmers to crop requirements. At the scientist to crop requirements level, users will examine crop requirements by combining climatic data and crop simulations, while at the farmers to crop requirement, rural small-scale farmers use the scientific information to plan for the farming season. The farmer then decides the planting and growing period as well as the harvest timing. The study suggests that building links between rural small-scale farmers, extension officers, and scientists makes sharing information easier. The proposed climate information framework design illustrates that stakeholders now have a personal relationship with climate information transmission and can influence the different actions to reduce the effect of climate change unpredictability. The study established that climate change and variability influence recent agricultural output trends. Recent temperature and rainfall trends could have resulted in low crop productivity in the study area. The study also reflected that women are a critical resource in agriculture and rural economy and that middle-aged men and youth are less interested in farming. The findings also showed that women are more receptive to climate information; hence, to promote access and use of climate information remains vital to consider gender-specific aspects for appropriate decision-making in the agriculture sector. The study also recommends enhancing the Farmers Research Group technique. In the past, the scope of this methodology has been narrowly focused, and it must be explored to incorporate additional types of climate information and more debate about how climate is related to the decisions farmers for implementation or consideration. , Thesis (PhD) -- Faculty of Science and Agriculture, 2022
- Full Text:
- Date Issued: 2022-02
- Authors: Pindura, Tineyi Herbert https://orcid.org/0000-0001-7233-6222
- Date: 2022-02
- Subjects: Farms, Small , Agriculture -- Environmental aspects , Climatic changes
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/22809 , vital:52784
- Description: In the Eastern Cape of South Africa, rural small-scale farmers live in uncertain times characterized by climate change and variability, which intensify social, political and financial inequalities. Therefore, there is a need to increase the understanding and interpretation of climate information to minimize crop production risk, reduce rural small-scale farmers’ vulnerability to climate, and maximize opportunities. Increasing the resilience among rural small-scale farmers requires appropriate and viable practical approaches. By using systems thinking approach (and the Raymond Mhlaba Municipality in the Eastern Cape as a study area), this research disseminates the complex nature of current climate information frameworks. Through inputs from farmers (through a Farmers Research Group methodology) and climate data, this thesis developed a new framework for communicating climate information (herein referred as the climate information communication systems framework) to rural small-scale farmers. The proposed climate information communication systems framework successfully integrates scientific and traditional knowledge. The framework constitutes certain stages, where the farming system and crop identification is the first stage. The second stage is the requirements stage, which has two relationships: scientist to crop requirements and farmers to crop requirements. At the scientist to crop requirements level, users will examine crop requirements by combining climatic data and crop simulations, while at the farmers to crop requirement, rural small-scale farmers use the scientific information to plan for the farming season. The farmer then decides the planting and growing period as well as the harvest timing. The study suggests that building links between rural small-scale farmers, extension officers, and scientists makes sharing information easier. The proposed climate information framework design illustrates that stakeholders now have a personal relationship with climate information transmission and can influence the different actions to reduce the effect of climate change unpredictability. The study established that climate change and variability influence recent agricultural output trends. Recent temperature and rainfall trends could have resulted in low crop productivity in the study area. The study also reflected that women are a critical resource in agriculture and rural economy and that middle-aged men and youth are less interested in farming. The findings also showed that women are more receptive to climate information; hence, to promote access and use of climate information remains vital to consider gender-specific aspects for appropriate decision-making in the agriculture sector. The study also recommends enhancing the Farmers Research Group technique. In the past, the scope of this methodology has been narrowly focused, and it must be explored to incorporate additional types of climate information and more debate about how climate is related to the decisions farmers for implementation or consideration. , Thesis (PhD) -- Faculty of Science and Agriculture, 2022
- Full Text:
- Date Issued: 2022-02
Parents and personnel’s partnership in early childhood education provisioning in the East London Education District
- Mudziwapasi, Lilymore https://orcid.org/0000-0002-7978-8499
- Authors: Mudziwapasi, Lilymore https://orcid.org/0000-0002-7978-8499
- Date: 2022-02
- Subjects: Education, Preschool -- Parent participation , Education, Preschool
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/21700 , vital:51743
- Description: Early childhood development is fundamental to human development and success in later life. Several stakeholders influence that development. Developmental policies are advocating for formal education provision at that early childhood age. Therefore, relationships and interactions of these stakeholders are of paramount importance in ensuring effective early childhood education provisioning – especially between parents and practitioners. Yet it has been reported that sharing of information concerning children’s educational development is not happening between parents and ECD practitioners. ECD centres are said to face many problems such as poor teaching and learning which may result in weak childhood educational development. Some of these challenges can be addressed through enhancing the partnership between parents and the ECD practitioners. The purpose of this study was therefore to explore the parents and practitioners’ partnership in early childhood education provisioning in ECD centres in the East London district. This study used the mixed method approach in the sampling, data collection and data analysis processes. The study focused on the partnership between parents and ECD practitioners, on how they work together, their views, how they communicate, on decision making and the strategies to enhance the partnership of parents and practitioners. Research questions in this study required both qualitative and quantitative data and analysis techniques. Quantitative data was collected from the ECD practitioners and principals by using questionnaires and qualitative data was collected through interviews with the parents. Quantitative data was analysed using SPSS and qualitative data was analysed using the thematic approach. The findings indicated that both parents and practitioners are working together in supporting learning and development. Parents are said to provide resources for use at the centre. Parents and practitioners are using different modes of communication for the learning and development of the children, including technology-based WhatsApp and emails. Practitioners are said to include parents in some decision making. The results indicated that even though some parents are working together with practitioners, while other parents are still showing ignorance and lack of knowledge on how they can partner for the educational development of the children. , Thesis (PhD) -- Faculty of Education, 2022
- Full Text:
- Date Issued: 2022-02
- Authors: Mudziwapasi, Lilymore https://orcid.org/0000-0002-7978-8499
- Date: 2022-02
- Subjects: Education, Preschool -- Parent participation , Education, Preschool
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/21700 , vital:51743
- Description: Early childhood development is fundamental to human development and success in later life. Several stakeholders influence that development. Developmental policies are advocating for formal education provision at that early childhood age. Therefore, relationships and interactions of these stakeholders are of paramount importance in ensuring effective early childhood education provisioning – especially between parents and practitioners. Yet it has been reported that sharing of information concerning children’s educational development is not happening between parents and ECD practitioners. ECD centres are said to face many problems such as poor teaching and learning which may result in weak childhood educational development. Some of these challenges can be addressed through enhancing the partnership between parents and the ECD practitioners. The purpose of this study was therefore to explore the parents and practitioners’ partnership in early childhood education provisioning in ECD centres in the East London district. This study used the mixed method approach in the sampling, data collection and data analysis processes. The study focused on the partnership between parents and ECD practitioners, on how they work together, their views, how they communicate, on decision making and the strategies to enhance the partnership of parents and practitioners. Research questions in this study required both qualitative and quantitative data and analysis techniques. Quantitative data was collected from the ECD practitioners and principals by using questionnaires and qualitative data was collected through interviews with the parents. Quantitative data was analysed using SPSS and qualitative data was analysed using the thematic approach. The findings indicated that both parents and practitioners are working together in supporting learning and development. Parents are said to provide resources for use at the centre. Parents and practitioners are using different modes of communication for the learning and development of the children, including technology-based WhatsApp and emails. Practitioners are said to include parents in some decision making. The results indicated that even though some parents are working together with practitioners, while other parents are still showing ignorance and lack of knowledge on how they can partner for the educational development of the children. , Thesis (PhD) -- Faculty of Education, 2022
- Full Text:
- Date Issued: 2022-02
Adsorption of antibiotic-resistant bacteria and their cell-free deoxyribonucleic acid harbouring resistance genes in drinking water with metal oxides
- Authors: Tobechukwu, Anthony Eric
- Date: 2022
- Subjects: Metallic oxides , DNA -- Synthesis , Drinking water
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/27787 , vital:69494
- Description: Access to cleaner water is essential to human health. The incidence of Antibiotic Resistant Bacteria ARB in drinking water and subsequent proliferation of Antibiotic Resistance Genes ARGs in drinking water is a concern for public health. The free DNA cassette harboring antibiotic resistance gene in drinking water has been linked with impaired public health. The ARGs allows bacteria to develop resistance towards antibiotics while ARB render antibiotics ineffective. Existing drinking water treatment technologies eg adsorption, ozonation and chlorination, have shown different levels of potency in the removal of conventional water pollutant. These technologies, which operating principles is based on oxidation or mass transfer, have been explored to gained an insight into their efficiency in the removal of ARB ARGs in water treatment. In general, methods that functions by mass transfer of the ARB ARGs is more effectively than bacteria oxidation. Consequently, adsorption technology was chosen using common metal oxide adsorbents. The adsorbents ZnOAg CeO2 and Al2O3 were synthesized via the self propagation combustion method. This method was selected because of the purity of the final product and the energy requirement. One of the challenges in the removal of ARB in drinking water is the release of ARGs. To address this concern, five different ZnOAg heterostructures were synthesized for the removal of Enterococci faecium. ZnOAg was chosen because of the bactericidal and bacteriostatic characteristics. The study revealed that the concentration of the precursors influences the microstructures of the adsorbents; however, it did not significantly affect the adsorption efficiency. The maximum adsorption capacity q34.11 CFUg was obtained for Ag1Zn3.5. The kinetic studies revealed that Ag1Zn1 and Ag1Zn2 adsorbents agreed to the pseudofirst-order kinetic equation and adsorbents Ag2Zn1 Ag3.5Zn1 and Ag1Zn3.5 agreed to the pseudo-second-order kinetic equation. Initial tap-water pH range was beneficial for the adsorption and the pH of the treated tap-water was within the WHO tap water recommendation 6.5 – 8.5 whereas the effect of ionic strength, anionic and cationic interference was insignificant in the adsorption of MDREF onto the different heterostructure. Interestingly the MDREF could retain its cell membrane integrity and resistance genes, suggesting that surface adsorption was the primary mechanism for the removal. Cerium IV oxide CeO2 was selected because of high adsorption towards phosphate, backbone base for DNA. To prevent the problem of antibiotic resistance, we have synthesized a CeO2 adsorbent that exhibit highly positive character in a wide pH range, via the simple self-propagation combustion protocol, for the removal of free DNA harboring antibiotic resistance genes. Molecular characterization of the extracted genes showed that the sizes for E. coli and inherent gyrB genes are 147 and 460 bp with a purity between 19 2.0. The XRD SEM TEM, and PZC results of the as-synthesized CeO2 showed an agglomerate of pure cubic-faced centered material and highly crystalline, with a net charge at pH 6.2. Experimental results revealed that the reaction proceeded via pseudo first-order kinetic, and it is governed by electrostatic attraction. The free- DNA solution pH electrolyte, and competing ions impacted on the adsorption process. Further experimental results showed that the as-synthesized CeO2 adsorbent has the potential to be used for the removal of free DNA harboring ARGs from tap-water even under oxic conditions. Alumina Al2O3 is an abundant adsorbent that has also shown high removal capacity towards phosphate. The highly pure synthesized Al2O3 adsorbent exhibit fluid-like behaviour under Scanning Electron Microscope SEM. The XRD pattern corresponds to αAl2O3. The adsorption kinetics was described by pseudo second orderadsorption capacity 11.7 μgg implying chemisorption, which agrees with the electrostatic force of attraction caused by opposing ions. This result was evident by the effect of different ions in the tap water. The synthesized α-Al2O3 has the potential for the removal of cell free DNA harbouring multiply resistant genes. , Thesis (MSc) -- Faculty of Science and Agriculture, 2022
- Full Text:
- Date Issued: 2022
- Authors: Tobechukwu, Anthony Eric
- Date: 2022
- Subjects: Metallic oxides , DNA -- Synthesis , Drinking water
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/27787 , vital:69494
- Description: Access to cleaner water is essential to human health. The incidence of Antibiotic Resistant Bacteria ARB in drinking water and subsequent proliferation of Antibiotic Resistance Genes ARGs in drinking water is a concern for public health. The free DNA cassette harboring antibiotic resistance gene in drinking water has been linked with impaired public health. The ARGs allows bacteria to develop resistance towards antibiotics while ARB render antibiotics ineffective. Existing drinking water treatment technologies eg adsorption, ozonation and chlorination, have shown different levels of potency in the removal of conventional water pollutant. These technologies, which operating principles is based on oxidation or mass transfer, have been explored to gained an insight into their efficiency in the removal of ARB ARGs in water treatment. In general, methods that functions by mass transfer of the ARB ARGs is more effectively than bacteria oxidation. Consequently, adsorption technology was chosen using common metal oxide adsorbents. The adsorbents ZnOAg CeO2 and Al2O3 were synthesized via the self propagation combustion method. This method was selected because of the purity of the final product and the energy requirement. One of the challenges in the removal of ARB in drinking water is the release of ARGs. To address this concern, five different ZnOAg heterostructures were synthesized for the removal of Enterococci faecium. ZnOAg was chosen because of the bactericidal and bacteriostatic characteristics. The study revealed that the concentration of the precursors influences the microstructures of the adsorbents; however, it did not significantly affect the adsorption efficiency. The maximum adsorption capacity q34.11 CFUg was obtained for Ag1Zn3.5. The kinetic studies revealed that Ag1Zn1 and Ag1Zn2 adsorbents agreed to the pseudofirst-order kinetic equation and adsorbents Ag2Zn1 Ag3.5Zn1 and Ag1Zn3.5 agreed to the pseudo-second-order kinetic equation. Initial tap-water pH range was beneficial for the adsorption and the pH of the treated tap-water was within the WHO tap water recommendation 6.5 – 8.5 whereas the effect of ionic strength, anionic and cationic interference was insignificant in the adsorption of MDREF onto the different heterostructure. Interestingly the MDREF could retain its cell membrane integrity and resistance genes, suggesting that surface adsorption was the primary mechanism for the removal. Cerium IV oxide CeO2 was selected because of high adsorption towards phosphate, backbone base for DNA. To prevent the problem of antibiotic resistance, we have synthesized a CeO2 adsorbent that exhibit highly positive character in a wide pH range, via the simple self-propagation combustion protocol, for the removal of free DNA harboring antibiotic resistance genes. Molecular characterization of the extracted genes showed that the sizes for E. coli and inherent gyrB genes are 147 and 460 bp with a purity between 19 2.0. The XRD SEM TEM, and PZC results of the as-synthesized CeO2 showed an agglomerate of pure cubic-faced centered material and highly crystalline, with a net charge at pH 6.2. Experimental results revealed that the reaction proceeded via pseudo first-order kinetic, and it is governed by electrostatic attraction. The free- DNA solution pH electrolyte, and competing ions impacted on the adsorption process. Further experimental results showed that the as-synthesized CeO2 adsorbent has the potential to be used for the removal of free DNA harboring ARGs from tap-water even under oxic conditions. Alumina Al2O3 is an abundant adsorbent that has also shown high removal capacity towards phosphate. The highly pure synthesized Al2O3 adsorbent exhibit fluid-like behaviour under Scanning Electron Microscope SEM. The XRD pattern corresponds to αAl2O3. The adsorption kinetics was described by pseudo second orderadsorption capacity 11.7 μgg implying chemisorption, which agrees with the electrostatic force of attraction caused by opposing ions. This result was evident by the effect of different ions in the tap water. The synthesized α-Al2O3 has the potential for the removal of cell free DNA harbouring multiply resistant genes. , Thesis (MSc) -- Faculty of Science and Agriculture, 2022
- Full Text:
- Date Issued: 2022