A process maturity framework of information security policy development life cycle
- Authors: Tuyikeze, Tite
- Date: 2014-12
- Subjects: Computer security , Information technology , Computer crimes
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/26797 , vital:66010
- Description: Information security policy development involves more than policy formulation and implementation. Unless organisations explicitly recognise the various steps required in the development of a security policy, they run the risk of developing policies that are poorly thought out, incomplete, redundant and, irrelevant and which will not be fully supported by the users. This study argues that an information security policy has an entire life cycle through which it must pass through during its useful lifetime. A content analysis on information security policy development methods was conducted using secondary sources in the relevant literature. The outcome of the content analysis resulted in the proposal of a framework of information security policy development and implementation. The proposed framework outlines the various steps required in the development, implementation and enforcement of an effective information security policy. A survey of 400 security professionals was conducted in order to evaluate the concepts contained in the framework.This study also emphasises the importance of integrating a security maturity assessment process into the information security policy development life cycle. A key finding of this study is the proposed maturity assessment framework which offers a structured methodology for evaluating the maturity level of an information security policy. The framework presents an integrated and holistic approach to ensure the incremental process maturity of the organisation’s information security policy development process. In addition, organisations using the proposed framework will be able both to determine the current maturity levels of their information security policy development process and also to plan enhancements in the correct sequence. , Thesis (PhD) -- Faculty of Management and Commerce, 2014
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Social intrepreneurship and millennium development goals in developing countries: case study of Zimbabwe
- Authors: Ngorora, Grace P K https://orcid.org/0000-0003-4756-313
- Date: 2014-11
- Subjects: Social entrepreneurship , Economic development , Poverty
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/26661 , vital:65847
- Description: The study set out to examine the contribution of social entrepreneurship to the achievement of Millennium Development Goals (MDGs), particularly MDG 1, the eradication of extreme poverty and hunger. Poverty reduction occurred when social entrepreneurial activities resulted in the improvement of the socio-economic well-being of social entrepreneurs and their beneficiaries. The problem this study sought to research on was that, despite the impact of social entrepreneurship, there has been inadequate attention to and discussion of its contribution to attaining the MDGs in Zimbabwe. The population were social entrepreneurs in Harare, Zimbabwe. The random sampling method was used to determine the sample size. Semi-structured questionnaires were used to collect primary data in Harare, Zimbabwe from 132 social entrepreneurs and 200 beneficiaries of social entrepreneurial activities. Secondary information was obtained from textbooks and various internet sources. The data collected was analyzed through SPSS Version 22 because of its appropriateness and wide use. The null hypothesis that social entrepreneurship does not contribute to the achievement of MDGs was rejected in favor of the alternative hypothesis that social entrepreneurship provides an alternative to the achievement of MDGs. Findings from the study suggest that social entrepreneurs contribute immensely to poverty reduction. They also contribute towards research and development, promoting gender equality and empowerment, education for all as well as access to health facilities. The segments of the population benefiting from social entrepreneurship include the poor, socially excluded, discriminated, the unemployed and disabled. The impact on poverty and hunger was achieved through microfinance initiatives, income generation activities, empowerment and capacity building. Results showed that social entrepreneurship activities solve social problems through providing food, shelter, water, education and collateral to access finance. The study concluded that social entrepreneurship is a plausible approach to promote implementation of policies to reduce extreme poverty and hunger by using readily available resources to bring sustainable solutions to problems. The strategies to make social entrepreneurship more effective included creating a conducive legal and policy environment, financial provision, political support, and government support, publicity of the contribution of social entrepreneurship, mentorship and collaboration among stakeholders. , Thesis (PhD) -- Faculty of Social Sciences and Humanities, 2014
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Opportunities and challenges for socio-economic transformation in rural Zimbabwe
- Authors: Mutami, Cephas
- Date: 2014-10
- Subjects: Economic development--Social aspects , Rural development , Community development
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/26459 , vital:65338
- Description: The focus of the thesis is the impediments of rural development in Zimbabwe and how opportunities for socio-economic transformation can be enhanced in the current setting. The study embarks from a conceptualization of rural poverty in Zimbabwe using theoretical and empirical evidence. Poverty in rural Zimbabwe has a historical legacy of deprivation and dispossession and is concentrated in communal areas which have low resources bases with low agriculture potential due to low rainfall and poor soils. The myriad of challenges in rural Zimbabwe point to the incoherent of rural development polices which are distorting markets for inputs and produce. Thus the study sought to address the rural development policy bottlenecks and develop a policy agenda for development.Using mixed methods approach, the study considered household economic conditions, their production practices and their vulnerability contexts in three selected districts which represent communal, old resettlement and small scale A1 and A2 areas. The household data is blended with interview data of rural development practitioners in both private and public agencies to produce comprehensive information on the impact of rural development policies. Archival research method was also used to do content reviews on government policy papers and programs so as to provide a holistic approach to analysis. Naturally mixed methods produce large volumes of data which requires thorough analysis. This was only possible through the use of analytical computer software packages for qualitative and quantitative data such as Atlas Ti and Excel spreadsheet, respectively. The study revealed that rural households in Zimbabwe are farmers. The majority of these farmers are smallholders who are located in remote communal areas and resettlement areas. Communal farmers constitute the bulk of rural citizens and live on less than a dollar a day. They are engaged in mixed farming methods and other non-farm activities especially during off-agriculture season. Communal households basically produce staples, with a low percentage producing cotton and groundnuts. Production choices and methods are shaped by subsistence needs of households but more so by risks and vulnerability contexts which they operate in. Farmers in resettlement areas face a different set of opportunities and threats to their livelihoods as they are located in prime agricultural land. However they are still challenged with the rural development approaches of central government. On the policy front, there is no pronounced rural development policy in Zimbabwe. The current setting has numerous pronouncements and programs which all seek to alleviate underdevelopment in Zimbabwe. These programs are replicating each other in practice and in most cases benefit the political elites and large scale commercial farmers. The multiplicity of government institutions which deal with rural development results in incremental decisions and programs which further impoverishes rural households. The study posits a rural development policy framework which is based on a comprehensive policy agenda which takes into cognizance the production requirements of households. Such a policy needs to be inclusive to the rural households in terms of governance and programs. A rural development policy agenda and framework would require the harnessing of resources from both the government and private players in well-articulated market friendly approaches. This would stimulate high productivity, enhance off-farm activities, raise incomes and foremost create appropriate social protection programs. Thus the potential for sustained economic growth and development in Zimbabwe lies with smallholder agriculture based rural development framework which can impact on the 67% population who live and work in rural areas. , Thesis (PhD) -- Faculty of Management and Commerce, 2014
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Assuring the quality of mentoring in Malawi's 1+1 model of initial primary teacher education programme: implications for teacher development
- Authors: Mwanza, Alnord Levison Dave
- Date: 2014-09
- Subjects: Mentoring in education , Total quality management
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/24632 , vital:63385
- Description: Teacher education has been the focus of numerous studies across the world. The debates revolve around what models or approaches to teacher development produce high quality teachers as measured by their ability to promote student learning. This has given rise to a continuum of models. At the one extreme there are those who advocate practice first and theory later; at the other there are those who recommend theory first and then practice. In between there is a combination of a variety of approaches. The models of teacher development, however, continue to grapple with the problem of how to balance theory and practice. In Malawi, the Ministry of Education, Science and Technology (MoEST) (MIE, 2006) came up with what they see as a balance between theory and practice when they introduced the 1 year of theory and 1 year of practice, a model referred to as the 1+1. The focus of this study was on the one year of practical teaching where mentoring is critical; hence the particular attention to examine and understand how its quality was assured from the perspective of fitness for purpose and fitness of purpose. The study was anchored mainly in the post-positivist paradigm and the methodology used was the mixed-methods approach. A mixed methods sequential explanatory design comprising a survey followed by a case study was employed. For the survey, four sets of semi-structured questionnaires whose Cronbach Alpha Reliability Coefficients, α, were 0.971, 0.827, 0.859 respectively were developed using the Tailored Design Method; these were pilot tested and then administered through a drop-and-pick strategy to a census sample of 804 respondents comprising 92 school mentors, 92 school head-teachers, 4 Teacher Training College mentoring coordinators and 616 student teachers. A total response rate of 96percent was obtained. The case study design phase comprised six one-on-one face-to-face structured interviews; five focus groups; five non-participant observations; and a review of ten documents employing interview schedules, observations and document checklists as instruments for data collection. A total of 27 participants, three of whom did not participate in the survey phase, were involved in this phase. Quantitative data collected from either phase was analyzed using IBM SPSS Statistics 20 while content analysis was used to analyse qualitative data. As a mixed-method study, some of the qualitative data was quantitized and some of the quantitative data was qualititized; and integration of data was made during the following stages: research questions formulation, data collection, data presentation, and discussion and interpretation. The study revealed several weaknesses in assuring the quality of mentoring in the 1+1 IPTE model. First, the norms and standards for mentoring designed by the Ministry of Education were not used to guide the mentoring process. Instead, they were designed to fulfil accountability requirements of the Ministry of Education and Teacher Training Colleges rather than self-improvement. Hence, the study revealed that all the 92 schools had, therefore, no policies, practice codes and standards for monitoring mentoring casting doubt on the functioning of internal quality assurance. The study further revealed that 12 of the school mentors (13percent) had lower academic qualifications than their seventy-two student teachers they were mentoring; 35 of the school mentors (38percent) were at the entry grade of their teaching career; two of the mentors (2percent) (mentoring over twelve student teachers) were within the first three years of teaching, which means that they, themselves, were in need of being mentored; and all the school mentors had full time teaching responsibilities. As such, some student teachers indicated that they were not being assessed at all by their school mentors. This situation regarding mentors raises concerns over the subject knowledge levels of the mentors, the role model functions of the mentors and their competency to effectively mentor student teachers, as well as concerns regarding the quality of the mentoring programme and its outcomes. Findings from the study also revealed little or no monitoring of mentoring in schools as schools had no structures and systems for monitoring mentoring; and heavy workloads and financial constraints prevented college lecturers from monitoring mentoring in primary schools. However, on a positive note, the study revealed that 99percent of school mentors were trained for their mentoring roles and were receiving regular training which was provided regularly on a term-basis. Based on the above findings, the 1+1 Initial Primary Teacher Education model in Malawi is undermined by weak mentoring practices which have the potential to widen the theory and practice gap, and potentially impact on the quality of teachers produced. It is further recommended that the mentoring practices move away from the technicist approach and focus on the actual processes of mentoring; that norms and standards for school mentor selection and support are developed; that there should be more workshopping, seminars and conferences for mentors; and that schools should be assisted to develop collegial-emancipatory quality assurance systems for mentoring. A rainbow model for assuring the quality of mentoring in the 1+1 IPTE mode is finally recommended. , Thesis (PhD) -- Faculty of Education, 2014
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Pills, Politics and Partners: NGOs and the Management of the HIV/AIDS Epidemic in the Eastern Cape, with special reference to the Lusikisiki Project, 2004-2014
- Authors: Govere, Fredrick Murambiwa
- Date: 2014-06
- Subjects: Non-governmental organizations -- South Africa -- Management , AIDS (Disease) -- Prevention , HIV-positive persons -- Services for -- South Africa
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/25720 , vital:64473
- Description: South Africa is one of the few countries in sub-Saharan Africa where HIV prevalence rates remain stubbornly high.1 This trend is both concerning and difficult to explain given that South Africa is economically well-resourced with a better health care infrastructure relative to other African countries. The African National Congress identified the HIV/AIDS epidemic as a major public health threat to South Africa as early as the 1990s (Parikh and Whiteside, 2007; Gevisser, 2007). In response, international donors provided a substantial amount of financial resources to support improvements in South Africa’s health care infrastructure. The persistently high rates of HIV/AIDS in the population beg for answers to questions such as why South Africa’s political leadership has been unable to control the spread of the disease and what particular social, behavioural and economic factors have contributed to South Africa’s disproportionate share of the global HIV/AIDS burden. At this point in time, public health experts still have not been able to definitively isolate the factors that explain the severity of the HIV/AIDS disease burden within South Africa’s population. While international donor aid continues to play a central role in the social, economic and health betterment in developing nations, its political impact on local governance structures has been much debated.2 In particular, the roles played by non-government organisations (NGOs) in the fight against HIV/AIDS in South Africa have been found to be complex, controversial, and their lasting value contested. Specifically debated is the disproportionate global investment in HIV/AIDS prevention, care and treatment in South Africa and the ways in which it has shaped intervention strategies, public and private policy, and the governance roles assumed by various national and local governmental agencies. Despite the threat of diminished financial support from international donor agencies that has loomed heavily over recipient countries in recent years, a number of key international donors have actually scaled up their global response to HIV/AIDS, particularly in South Africa.3 These donor agencies include the U.S. government’s Presidential Emergency Plan for AIDS Relief (PEPFAR) and the Global Fund, as well as non-profit humanitarian organisations such as Mèdecins Sans Frontiéres (MSF), commonly known as Doctors without Borders. This study aims to critically examine the prevailing intervention strategy used by these international donors to “push the pill;” that is, to push a public health agenda that holds antiretroviral (ARV) therapy as the preeminent solution to fighting the HIV/AIDS epidemic at the expense of other intervention strategies. While a biomedical approach remains the most compelling intervention strategy, the fight against HIV/AIDS needs to be more comprehensive in its scope taking into account local knowledge and culture. This study will discuss how political rhetoric delivered through the media and monitoring, evaluation, and reporting systems has been used to embed a biomedical pill agenda into the organizational culture and intervention strategies implemented by local community-based organizations. Through a process of strategic translation, HIV/AIDS has been portrayed as the number one health problem facing South Africa today; in fact, across all of Sub-Saharan Africa (World Health Organisation 2005a, 2005b). As such, the magnitude of the HIV/AIDS epidemic has justified the focus on making ARV drugs available throughout Sub-Saharan Africa at the expense of other interventions that could aim more precisely at the key social and health problems faced by the South African population which compromise health and well-being. The question about whose interests are donors and donor-funded agencies representing, and what are the intended and unintended consequences that result from these interests is the subject of this study. The study will attempt to shed light on these questions through a critical examination of the widely publicized HIV/AIDS intervention programme, the Lusikisiki Project, located in the Eastern Cape Province of South Africa. I have drawn extensively from the development discourse literature and such scholars as Ferguson, 1990; Escobar, 1994 and 1995; Rist, 1997; and Mosse, 2005, to provide theoretical grounding for answering the questions posed.4 The study will critically analyse the social and political factors that defined this reputedly successful HIV/AIDS intervention project. Further, the study wil elucidate other cultural and behavioural factors that shaped the initiative in its battle against HIV/AIDS. , Thesis (PhD) -- Faculty of Management and Commerce, 2014
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Problem-based learning strategies as determinant of grade 9 student's academic achievement in algebra
- Authors: Olaoye, Olabisi Fatimat
- Date: 2014-04
- Subjects: Problem-based learning , Learning strategies
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/24717 , vital:63535
- Description: Many scholars in the field of mathematics education have written extensively on algebra and the reasons why algebra remains one of the aspects of mathematics that usually poses problems to students. This study examined the effects of problem based learning strategies on students’ academic achievement in algebra using language proficiency and gender as moderator variables. A 3X2X3 pretest-posttest control group quasi-experimental design was adopted in the study. One hundred and nine subjects were involved in the study (109) this consisted of forty-four males and sixty-five females in four Grade 9 mathematics classes in East London District. The study was carried out in two schools using two intact classes in each of the school. One class in each school was randomly assigned to the experimental and another to control groups. Four instruments were constructed and used for this study. These are: Language Proficiency Achievement Test (LPAT), Problem Based Learning Strategies are in two parts (PBLSa) and (PBLSb), Conventional Teaching Guide (C.T.G). The instruments were validated and used for data collection before and after the four week experiment. Seven hypotheses were generated and tested in the study at 0.05 alpha levels. The data collected were subjected to both descriptive and inferential statistics, which included Analysis of Covariance (ANCOVA) of students post-test achievement and where the results were significant, Pairwise Comparison Analysis of Scheffe post-hoc were employed to detect the source of variation and the direction of significance of post-test achievement according to treatment groups. One of the major findings of this study was that problem based learning strategies (PBLSa), (PBLSb) are more effective than conventional method in students’ academic achievement in algebra ( x = 3.05; SE =.720), ( x = 4.23; SE=.714), ( x = 3.36; SE =.726) in that order. The study also revealed there is no significant main effect of treatment and language proficiency on students’ academic achievement in Algebra (F (2,109) = .926; p>.05). Also, there is no significant main effect of treatment and gender on students’ academic achievement in algebra. (F (1.109) =.237; p>.05). However, this study recommended that problem based learning strategies should be embraced by both the teachers and learners in order to enhance students’ academic achievement in algebra. , Thesis (PhD) -- Faculty of Education, 2014
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Radio broadcasting, policy and local language revitalisation in Zimbabwe
- Authors: Mabika, Memory https://orcid.org/0000-0002-4499-6300
- Date: 2014-01
- Subjects: Broadcasting -- Zimbabwe , Broadcasting policy -- Zimbabwe
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/25789 , vital:64479
- Description: The media as disseminators of news are considered the principal institutions that have the mandate to provide relevant information to the citizenry; from which members of the public get a better understanding of their society. Communicating in indigenous languages, in particular, facilitates interaction and the preservation of African languages. Studies have shown that radio is the most effective channel of mass communication which can help in the usage and preservation of indigenous languages. This is because it is affordable and accessible in more peripheral areas than other media. However, due to radio’s ideological and hegemonic functions, many governments in most African countries and in Zimbabwe, in particular, have continued to closely monitor and control its day to day running. Zimbabwe radio, due to the previous and current political contexts, has continued to create an atmosphere where minority languages are not given necessary recognition leading to their exclusion from most of the existing radio stations. This has come to threaten indigenous minority languages and to weaken the long established cultures. The research springs primarily from the desire to unearth the truth behind the failure by ILRBSs and government to protect indigenous languages in Zimbabwe, a sovereign nation. The electronic colonialism theory and globalisation approach (specifically its cultural globalisation strand) were utilised in this study. The research adopted a mixed method design. Data was collected using four instruments namely, Interviews, Focus groups discussions, questionnaire and document analysis. The study revealed that minority languages in Zimbabwe have never been developed beyond their oral use by speakers in their confined locations. This makes any effort to use these languages for broadcasting a serious challenge since speakers and non-speakers do not value these languages. The government’s stringent media laws and policies have made the usage of radio broadcasting in preserving indigenous languages an almost impossible task. The ambiguous BSA and its various vague policies are nothing but a fallacy to minority indigenous languages’ revitalization efforts. In addition, the existing education system is worsening this problem because of its failure to develop educational materials in minority indigenous languages. The study concludes with the observation that in multilingual and multicultural Zimbabwe, the language stance of the government, education system and the media particularly radio are destructive to indigenous languages preservation and revitalisation. In order to ensure effective indigenous languages preservation and revitalization in Zimbabwe, there is need to revise the various language policies enshrined the BSA and to open up the broadcasting landscape to reflect the multiplicity and diversity of voices existing in the country. , Thesis (PhD) -- Faculty of Social Sciences and Humanities, 2014
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The role of indigenous knowledge in agriculture and environmental conservation: the case of Gutu District Zimbabwe
- Authors: Wutete, Obert
- Date: 2014-01
- Subjects: Traditional ecological knowledge , Conservation of natural resources , Nature conservation
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/26526 , vital:65520
- Description: Indigenous Knowledge IK is an emerging area of study that focuses on a community’s ways of knowing, seeing and thinking, developed over a long period of time. The knowledge is passed down orally from one generation to the other. Therefore, this study highlights a number of important factors that are associated with the use of IK in rural communities such as Gutu district. The major importance of IK include the following: it reduces costs through the use of locally available resources, acts as the basis of local-level decision making, is familiar and acceptable to the communities in which the knowledge exists and also that IK helps communities to develop their own sources of livelihoods. The objectives of the study were; to identify the types of IK peculiar to agriculture practices and the conservation of the environment within Gutu district; establish the challenges confronting IK utilization in communities; identify the constraints affecting the sharing, transfer and preservation of IK; determine the measures which should be adopted in order to promote the appreciation and value placed on IK in agriculture practices and the conservation of the environment; and to propose recommendations on how IK should be utilized for sustainable livelihoods in agriculture and environmental conservation in Gutu district and other rural communities. The study employed the survey method which was premised on the use of multiple data gathering tools, also referred to as triangulation. A total of 100 interviewees were sampled from Gutu communities for face-to-face interviews, 48 participants for focus group discussions, with each group comprising of 6 participants. The researcher collected data using a set of interview guide questions for individual participants as well as for the focus group discussions. In order to be comprehensive in data collection, the researcher also utilized observations and transects walks (these are random walks done in an area under study in order to have a better understanding of activities of the area). The triangulation of data gathering tools assisted with diverse information on the crops cultivated by residents, cultivation methods, harvesting and the preservation of the crops. The researcher also gathered information on how residents interacted with the environment, established reasons which contributed to the deterioration of the environment, established the sacredness associated with certain areas in Gutu district and the use of taboos as a way to safeguarding natural resources. The traditional practices have become a thing of the past to the present generations. The research had four major findings: First, it was found out that Indigenous Knowledge which was the basis of traditional lifestyles is no longer a major practice by Gutu residents. The gradual decline of traditional indigenous practices contributed among other factors to the decline in agriculture output. Residents have abandoned traditional farming methods such as conservation farming. The latter practice kept the soil intact and rich in fertility unlike the use of ploughs and tractors which weakens the soil. More so, residents have opted for the cultivation of crops which do not give them good harvests. Maize is one such crop which suffers from droughts as opposed to small grain crops, comprising of finger millet, pearl millet and sorghum. These endure dry conditions with limited rainfall. Gutu residents also revealed that there is overlooking of the sacredness associated with certain areas and the use of taboos to conserve the environment. It was further established that while residents have knowledge of sacred places and prohibitive taboos which are meant to safeguard the environment, residents no longer respect the prohibitions due to the influence of modernity and Christianity which regard some of the practices as backward or wayward beliefs. Second, the study established that the influence of colonial policies influenced a number of changes to the continued utilization of Indigenous Knowledge in agriculture and the management of the environment. For example, Gutu residents no longer practice shifting cultivation which used to be practice in the 1920s backwards. Residents are now confined to small pieces of land which are continuously cultivated on a yearly basis. Therefore, soils have become exhausted and production has gone down since the majority of residents are not able to replenish the soil through the use of fertilizer whose cost they cannot afford. It was also established that the environment has deteriorated continuously due to over use by an ever-increasing population. Third, the study found out that traditional platforms such as the dare men’s meeting place are no longer in existence. The dare is a set-up in which males sit around a fire place and shared ideas on different subject areas as a way of teaching one another life lessons and grooming the future generations. On the other hand, women use the round hut in which food is prepared to address a number of teachings to the girl child. Elderly women took the opportunity to induct young girls into adulthood along the duties expected of women in the society. Nowadays, both males and females share the hut used for preparing food and this is disrupting the traditional set-up of inducting young males and females in separate and appropriate platforms. The situation was worsened by colonialism which came along with Christian teachings, part of which were not in tandem with traditional indigenous practices. For example, the sacredness of places and the use of taboos are regarded by the youths as old and outdated beliefs with no relevance in today’s modern world. To make matter worse, there is limited contact time between the youths and the elders due to work commitments in urban areas. This has limited the opportunities for the sharing, transfer and preservation of IK as opposed to the traditional times in which shared communal lives allowed constant interactions and appreciation of indigenous ways of life. Fourth, there are no tangible strategies for the preservation of IK for posterity. While general information on traditional IK can be shared by residents, there are individuals who possess rare knowledge but made it their preserve after they realized that the knowledge offered them competitive advantages and benefits. Another aspect is that the youths have lost interest in IK of the past since they feel that the knowledge has lost relevance to their present interests due to the dynamism of culture. In the youths’ views, IK which is useful and relevant to their generation will continue to exist while practices which are no longer useful will fall by the way side. The research utilized interviews in order to establish the manner in which Indigenous Knowledge is acquired, shared, transferred and even retained for use by future generations. The findings of the study formed the basis for recommendations, of which if adopted, could contribute significantly to the revival of indigenous knowledge practices in Gutu district. The use of indigenous knowledge, peculiar to people in a given locality, can contribute to the promotion of sustainable livelihoods and development of rural communities in Gutu district. Based on the findings of the study, the following recommendations can be considered there is need for the establishment of Indigenous Knowledge Resource Centres IKRCs in communities so that individuals with interest in traditional IK can acquire the knowledge free of charge. It may also be beneficial if IK can be introduced to become part of the tertiary level curricula. Since Zimbabwe is an agro-based economy and also the fact that rural communities rely on the environment for provisions such as firewood, production and conservation measures being part of the curricula. This will assist decision makers to appreciate and value IK in decision making and development projects, not only in agriculture and the conservation of the environment, but also in other sectors that promote people’s livelihood. , Thesis (MA) -- Faculty of Social Sciences and Humanities, 2014
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Secularization processes in Malawi and Great Britain: a comparative inquiry
- Authors: Gama, Billy Lickson
- Date: 2013-12
- Subjects: Secularization (Theology)
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/25644 , vital:64346
- Description: The major purpose of the study was to critically examine the applicability of manifestations and factors of secularization in Britain to Malawi. In examining the manifestations and factors the study had to examine the process of secularization in Britain and its causal factors using theoretical frameworks and examine manifestations and causal factors of secularization in Malawi using field research work as a major source supported by the theoretical frame works of secularization. The study was guided by the key research question, “Are the manifestations and factors of secularization in Britain applicable to Malawi?” The question was supported by other follow up questions, namely, “What were the factors that contributed to the rise of secularization in Britain?” “What is the connection between Britain and Malawi?” “To what extent does secularization in Britain affect that in Malawi?” “Does Malawi have unique factors that are specific or are the same factors at work that have contributed to the process of secularization in Britain?” The conceptual framework for this research was derived from the literature on “secularization” from different scholars, in particular, the work of Dobbelaere (2002) whose analysis of secularization provided the theoretical frame work for this study. Learning from Dobbelaere (2002) the study used three types of secularizations, namely, societal, organizational and individual. Dobbelaere (2002:24ff) distinguishes the three types of secularization in a very simple way: individual secularization, the lack of individual practice, societal secularization, the loss of influence on society, and organizational secularization as the effect on religious organizations by changes in society. The methodological approach to the study was the mixed methods approach that involved three types of research methodologies: the qualitative, quantitative and case studies approaches. Data was collected through personal and focus group interviews. It was supported by secondary sources which include books, minutes of meetings in various institutions and the internet. The study notes that all the three types of secularization are being experienced in Malawi; societal secularization has been manifested by the removal of religious connected syllabus in schools, suspension of homosexuality laws and involvement of religious leaders in different immoral issues just like non-believers. Organizational secularization has been manifested by fights among religious institutions e.g. boarder disputes that have been given as case studies in chapter five and finally the individual secularization is being manifested in Malawi as membership records indicate that there is a big decline in church attendance and participation in religious activities. New knowledge that this study is proposing is that secularization is manifested in Malawi through the partial influence from Britain because of strong links between the two countries and the effects of modernization. The study further proposes that donor influences, Islam, lack of well educated religious leaders and African Traditional Religion are the unique factors of secularization in Malawi, while modernization, urbanization, industrialization are factors that are partially applicable to Malawi, and religious pluralism is fully applicable to both Britain and Malawi as a factor of secularization. Finally the study proposes that a proper training of religious leaders is vital for the sustainability of the church as it experiences the manifestations of secularization. , Thesis (PhD) -- Faculty of Social Sciences and Humanities, 2013
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Assessment of the physicochemical and microbiological qualities of Tyume River in Amathole District in the Eastern Cape Province, South Africa
- Authors: Sibanda, Timothy https://orcid.org/0000-0002-6864-3796
- Date: 2013-05
- Subjects: Water -- Purification , Sewage -- Purification
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/24465 , vital:62812
- Description: The bioflocculant-producing potentials of three marine bacteria isolated from the sediment samples of Algoa Bay in the Eastern Cape Province of South Africa were assessed. Analysis of the partial nucleotide sequence of the 16S rDNA of the bacteria revealed 99 percent, 99 percent, and 98 percent similarity to Cobetia sp. L222, Bacillus sp. A-5A, and Bacillus sp. HXG-C1 respectively and the sequence was deposited in GenBank as Cobetia sp. OAUIFE, Bacillus sp. MAYA and Bacillus sp. Gilbert (accession number JF799092, JF799093, and HQ537128 respectively). Cultivation condition studies for Cobetia sp. OAUIFE revealed that bioflocculant production was optimal with an inoculum size of 2 percent (v/v), initial pH of 6.0, Mn2+ as the metal ion, and glucose as the carbon source. Metal ions, including Na+, K+, Li+, Ca2+and Mg2+ stimulated bioflocculant production resulting in flocculating activity of above 90 percent. This crude bioflocculant is thermally stable, with about 78 percent of its flocculating activity remaining after heating at 100 oC for 25 min. Analysis of the purified bioflocculant revealed it to be an acidic extracellular polysaccharide. FTIR analysis revealed the presence of methoxyl, hydroxyl, and carboxyl - groups in the compound bioflocculant and SEM micrograph of the bioflocculant revealed a crystal-linear structure. On the other hand, bioflocculant production by Bacillus sp. MAYA was optimal when glucose (95.6 percent flocculating activity) and ammonium nitrate (83.3 percent flocculating activity) were used as carbon and nitrogen sources respectively; inoculum size was 2 percent (v/v); initial pH 6; and Ca2+ as coagulant aid. Chemical analysis of the purified bioflocculant shows that it is composed of uronic acid, neutral sugar and protein. FTIR analysis also revealed the presence of methoxyl, hydroxyl, carboxyl and amino- groups in this bioflocculant. The bioflocculant is thermostable with about 65.6 percent residual flocculating activity retained after heating the bioflocculant at 100 oC for 25 min. However bioflocculant production by Bacillus sp. Gilbert was optimal when sodium carbonate (95.2 percent flocculating activity) and potassium nitrate (76.6 percent flocculating activity) were used as carbon and nitrogen sources respectively; inoculum size was 3 percent (v/v); initial pH 9; and Al3+ as cation. The crude bioflocculant retained 44.2 percent residual flocculating activity after heating at 100 oC for 15 min. FTIR analysis reveals the presence of hydroxyl, carboxyl and methylene - groups in the compound bioflocculant. SEM micrograph of the bioflocculant revealed an amorphous compound. The consortia of these bacteria strains also produced bioflocculants with high flocculating activities which were highly efficient in removing turbidity and chemical oxygen demand (COD) from brewery wastewater, diary wastewater and river water. The bioflocculants from the consortia seemed better than traditional flocculants such as alum . The characteristics of the bioflocculant produced by the consortium of Cobetia sp. OAUIFE and Bacillus sp. MAYA showed that this extracellular bioflocculant, composed of 66percent uronic acid and 31percent protein and an optimum flocculation (90 percent) of kaolin suspension, when the dosage concentration was 0.8 mg/ml, under weak alkaline pH of 8, and Ca2+ as a coagulant aid. The bioflocculant is thermally stable, with a high residual flocculating activity of 86.7 percent, 89.3 percent and 87.0 percent after heating at 50 oC, 80 oC and 100 oC for 25 min respectively. The FTIR analysis of the bioflocculant indicated the presence of hydroxyl, amino, carbonyl and carboxyl functional groups. Scanning electron microscopy (SEM) image revealed a crystal-linear spongy-like bioflocculant structure and EDX analysis of the purified bioflocculant showed that the elemental composition in mass proportion of C,N,O,S and P was 6.67:6.23:37.55:0.38:4.42 (percent w/w). However, the characteristics of the bioflocculant produced by the consortium of Cobetia sp OAUIFE and Bacillus sp. Gilbert showed an optimum flocculation (90 percent) of kaolin suspension when the dosage concentration was 0.2 mg/ml, under neutral pH of 7, and Ca2+ as a coagulant aid. The FTIR analysis of the bioflocculant Tyume River water samples were collected monthly, over a 12-month period starting from August 2010 and ending in July 2011, and transported on ice to the Applied and Environmental Microbiology Research Group (AEMREG) Laboratory at the University of Fort Hare, Alice for analyses within 6 h of collection. Electrical conductivity (EC), total dissolved solids (TDS), temperature, pH and dissolved oxygen (DO) of water samples were determined in situ using a multi-parameter ion-specific meter. Concentrations of orthophosphate and total nitrogen (nitrate + nitrite) were determined by standard photometric methods. Total coliforms (TC), faecal coliforms (FC) and enterococci were determined by the membrane filtration method. Viruses in water samples were concentrated using the adsorption-elution method, followed by extraction of viral nucleic acids and purification done using commercially available kits. The concentrations of human enteric viruses in the river-water samples were estimated using quantitative PCR. RNA viruses were quantified in a two-step protocol where RNA was first transcribed into cDNA in a separate reverse-transcription step. Adenovirus species and serotypes were simultaneously detected using serotype-specific multiplex PCR. Norovirus genogroups GI and GII were detected by semi-nested PCR. The risk of infection associated with recreational and domestic use of the water was also estimated. Biochemical oxygen demand (BOD) levels fell within the stipulated BOD guideline of 10 mg/ℓ for surface waters where full contact use is allowed and ≤ 30 mg/ℓ where public access is prohibited, restricted, or infrequent. DO concentrations generally ranged between 7.47 mg/ℓ and 10.42 mg/ℓ, well within the target water quality requirements. The temperature regime ranged between 6°C and 28°C and for most sampling sites, the temperature regimes were within the acceptable limit of no risk (≤ 25°C) for domestic water uses in South Africa. EC ranged between 47 μS/cm and 408 μS/cm well within the South African target water quality EC guideline of 700 μS/cm though it was observed to increase as the river flowed through settlements. The pH in the period beginning September 2010 through to January 2011 was consistently below pH 9, but from February 2011 to June 2011 the pH significantly increased to between pH 10 and pH 11 at most sampling sites. Unpolluted waters normally show a pH of between 6.5 and 8.5. Most of the pH values observed in this study lie between pH 8.5 and pH 10.8 levels which are not far off from the upper level guideline of pH 9.0 for domestic use. Turbidity ranged between 6 NTU and 281 and fell short of the target water quality range (0 NTU to 1 NTU) of no risk for domestic water uses in South Africa. Monthly TDS values and EC values showed direct proportionality. TDS concentrations at all sites fell within the acceptable guideline of 0 mg/ℓ to 450 mg/ℓ of TDS for domestic use. Nutrient profiles were as follows: nitrate (0.18 mg/ℓ to 4.21 mg/ℓ); nitrite (0.02 mg/ℓ to 2.35 mg/ℓ); and orthophosphate (0.06 mg/ℓ to 2.72 mg/ℓ). The bacteriological qualities of the water were poor, exceeding the guideline of 200 CFU/100 mℓ and 33 CFU/100 mℓ for FC and enterococci respectively, for recreational water. FC counts also exceeded the 1 000 CFU/100 mℓ guideline for water used in fresh produce irrigation. Generally, higher counts of TC, FC and enterococci were recorded at the sampling sites located at the lower reaches of the river compared to the upper reaches. Adenovirus was detected in 31percent of the river samples in concentrations ranging between 1.0×100 genome copies/ℓ and 8.49×104 genome copies/ℓ. Serotyping showed the presence of species C adenovirus serotypes 1, 2, 6 and 7, and species F adenovirus serotype 41. The prevalence of norovirus was 4percent while rotavirus was detected in 4percent of river samples in concentrations ranging between 9×100 genome copies/ℓ and 5.64×103 genome copies/ℓ. Hepatitis A virus was detected in 13percent of river samples in concentrations ranging between1.67×103 and 1.64×104 genome copies/ℓ while enteroviruses were not detected. Detection of enteric viruses was inversely correlated to temperature. Risk analysis showed that both hepatitis A virus and adenovirus presented significantly higher risk of infection values compared to rotavirus in the case of ingestion of 10 mℓ or 100 mℓ of water from Tyume River while enteroviruses did not present any significant risk of infection. Tyume River water samples also did not conform to the US Environmental Protection Agency (US EPA) bacterial criteria of 200 CFU/100 mℓ faecal coliforms and 33 CFU/100 mℓ enterococci for bathing waters. Whereas the physicochemical parameters showed that Tyume River water was relatively clean, the bacteriological water quality was poor. Most of the microbiological contamination observed in this study (especially FIBs) can be blamed on inadequate sanitary infrastructure as we observed that open defecation is commonplace in this catchment, which also serves as a conduit for effluent discharges from wastewater-treatment facilities. Enteric viruses were detected along the course of the river in a sporadic pattern, generally not related to natural hydrological cycles and so we conclude that the presence of enteric viruses in the river is suggestive of the dynamics of the same in the host population. Even though the proportion of infective viruses was estimated in this study, fact remains that there is considerable risk of infection posed by the use of raw surface water for either domestic or recreational use. This study further confirmed the lack of correlation between faecal indicator bacteria and enteric virus occurrence in environmental waters, showing that assaying for enteric viruses in environmental waters remains the best method for determining the health risks associated with the use of faecally contaminated water. , Thesis (PhD) -- Faculty of Science and Agriculture, 2013
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A study of the implementation of public policy to increase participation in recreation sports in Lagos state: Nigeria
- Authors: Setonji, Noel Adeoye https://orcid.org/0000-0002-2101-8037
- Date: 2013-04
- Subjects: Public administration
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/24035 , vital:62301
- Description: The study investigated the extent to which implementation of the current public policy had improved recreation sport services and participation in Lagos metropolis of Lagos State-Nigeria. The study was delimited to 14 out of 16 Local Governments in Lagos metropolis. The study participants were 650 (334 males and 316 females) selected from 14 Local Governments in Lagos metropolis. Stratified random sampling technique was applied to get the volunteered metropolitan Lagos Local Government staff participants used for the study. Mixed research approaches involving quantitative and qualitative data collection were employed to carry out the study. Pilot study was carried out and reliability of each instrument was established with Cronbach’s alpha and test-re-test after an interval of two weeks. The reliability coefficients based on Pearson’s Product Moment Correlation were PRSPIQ (0.89), PRSPIBQ (0.87), RSFQ (0.79), and EPIMCEQ (0.85). The quantitative data collected were entered into Microsoft Excel 2007 Software and later exported to SPSS 18.0 statistical package for data analysis. Simple frequencies of responses in % and factor analysis were used in analysing the data collected, while Chi-square test of goodness-of-fit was used to test all the four hypotheses. The interview data collected were analysed using inductive method. All the hypotheses were pitched at 0.05 confidence level. Results of the study revealed that: implementation of the current public policy had not significantly improved recreation sport services and participation in Lagos metropolis: x2 (4, N = 650) = 395000, p<.0001, was significant at p<0.05); Barriers had affected effective implementation of the current public recreation sports policy in Lagos metropolis: x2 (4, N = 650) = 38.6000, p<.0001, was significant at p<0.05); implementation of the current public policy had not improved recreation sport facilities and safe recreation environments in Lagos metropolis: x2 (4, N = 650) = 33.3000, p<.0001, was significant at p<0.05; implementation of the current public policy had not effectively enhanced management competencies of recreation sport officers in Lagos metropolis: x2 (4, N = 650) = 64.3000, p<.0001, was significant at p<0.05. The conclusions drawn from the findings of the study include: the current public policy had not significantly improved recreation sport services in Lagos metropolis. Specifically, the study found that: the current policy had not done enough to enhance increased participation in recreation sport activities in Lagos metropolis; barriers had affected effective implementation of the current public recreation sports policy in Lagos metropolis; the public policy had not done enough to improve recreation sport facilities and safe recreation environments in most parts of Lagos metropolis; and the current public policy had not effectively enhanced management competencies of public recreation sport officers in Lagos metropolis. The study findings had it too that to effectively implement public recreation sports policy in Lagos metropolis, stakeholders should be adequately involved in the implementation activities. Based on the study findings, the study among others recommends that the State Government should be: proactive to update the content of the current public policy on recreation sports as the needs of the users are increasingly changing over time; Government agents, private organizations, recreation sport professionals and community stakeholders should be actively involved in the implementation processes to forestall any barriers; more recreation facility pools in safe and conducive environments should be provided and assessable to increase participation in recreation sports by the people of Lagos metropolis; and the public policy should be effectively implemented to enhance management competencies of public recreation sport managers in Lagos metropolis. , Thesis (PhD) -- Faculty of Management and Commerce, 2013
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Assessment of the water quality, incidence of enteric viruses and microbial risk in the Buffalo River in the Eastern Cape Province of South Africa
- Authors: Chigor, Vincent Nnamdigadi https://orcid.org/0000-0002-0811-4526
- Date: 2013-03
- Subjects: Water quality , Water -- Microbiology
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/24272 , vital:62596
- Description: Buffalo River is an important water resource in the Eastern Cape Province of South Africa. Over a 1-year period (August 2010–July 2011), the water quality and incidence of human enteric viruses (HEntVs) was assessed, using standard methods and molecular techniques and a total 72 composite water samples collected monthly from a total of 6 sites located on the river and three dams along its course. The sites were selected based on a number of factors including geographical location, anthropogenic activity/major water use, rural/urban status and access. A total of 13 physicochemical parameters were determined using the standard methods. The counts of faecal indicator bacteria (FIB) including total coliforms (TC), faecal coliforms (FC) and enterococci (ENT) were determined by the membrane filtration technique. HEntVs were concentrated using an adsorption-elution method based on cation (Al3+)-coated membrane filter. Real-time quantitative polymerase chain reaction (qPCR) was used for the detection and quantification of human adenoviruses (HAdV), and real-time reverse transcriptase-PCR (RT-qPCR) was used for the quantitative detection of hepatitis A virus (HAV), human rotaviruses (RoV) and enteroviruses (EnV). The detected HAdV were characterized by multiplex conventional/semi-nested PCR methods. The risks for human health constituted by exposure to the detected HEntVs at the six sites were evaluated by a static quantitative microbial risk assessment (QMRA) using both the exponential and beta-Poisson models. Water temperature ranged from 11 to 28oC, while pH varied from 6.6 to 10.7, and turbidity from 1.7 to 133 NTU. Electrical conductivity (EC), total dissolved solids (TDS) and salinity showed drastic variations (42.3-46693 μS/cm, 20.3–23350 mg/L and 0.02–33.8 PSU respectively). The concentrations of other parameters ranged as follows: chloride (3.7–168 mg/L); DO (6.9–11.1); BOD (0.6–9.4); COD (3.7–45.9); nitrite-nitrogen (0.02–0.21); nitrate-nitrogen (1–4.47); and orthophosphate (0.01–1.72). TC, FC and ENT counts were high and ranged from 1.9 × 102–3.8 × 107 cfu/100 mL, 0–3.0×105 cfu/100 mL and 0–5.3 × 105 cfu/100 mL for TC, FC and ENT respectively. Significantly (P<0.05) higher concentrations of FC and ENT were observed at the sampling sites located at the lower reaches of the river compared to the upper reaches. The FIB counts mostly exceeded the maximum limits recommended by national and international guidelines for safe fresh produce irrigation, domestic applications, full-contact recreation and livestock watering. Significant (P<0.01) positive correlations existed between TDS and salinity (r=0.921), between turbidity and each of TC (r=0.552) and FC (r=0.425), as well as between BOD and each of TC (r=0.282), FC (r=0.472) and ENT (r=0.552). Phosphate correlated positively with FC (r=0.424), and nitrate also with the same, FC (r=0.460). A strong positive correlation existed between FC and ENT (r=0.915). There existed a significant (P˂0.01) inverse correlation between enteric viruses and each of water temperature (r=-0.191) and pH (r=-0.234). No correlation could be deduced between enteric viruses and all the tested chemical and bacteriological parameters. HAV, HAdV, RoV and EnV were detected in 43.1percent, 34.7percent, 13.9percent and 9.7percent respectively of the total 72 water samples tested. Two or more viruses were detected in 22.2 percent of the samples. HAdV were detected at 5 of the 6 sampling sites with concentrations ranging from 1.2×101 genome copies (GC)/litre to 4.71×103 GC/litre. Epidemiologically important serotypes, Ad40/41 constituted 83.3percent, while Ad21 made up 16.7percent of all the HAdV detected. HAV was detected at all the sites in significantly (p < 0.05) varying concentrations that ranged from 1.5 × 101–1.9 × 105 GC/litre compared to RoV and EnV. Neither of RoV nor EnV was detected at any of the dams. The detected concentrations at the non-dam sites ranged from 2.5 × 101–2.1 × 103 GC/litre and 1.3 × 101–8.6 × 101 GC/litre for RoV and EnV respectively. The values for the estimated daily risks of enteric virus infection varied with sites and exposure scenario, and ranged from 7.31×10-3–1 (for HAdV), 4.23×10-2–6.54×10-1 (RoV), 2.32×10-4–1.73×10-1 (HAV) and 1.32×10-4–5.70×10-2 (EnV). The yearly risks of infection in individuals exposed to the river/dam water via drinking, recreational, domestic or irrigational activities were unacceptably high, exceeding the acceptable yearly risk of 0.01percent (10-4 infection/person/year) recommended by the USEPA for drinking water. The risks of illness and of death from infection ranged from 6.58×10-5–5.0×10-1 and 6.58×10-9–5.0×10-5 respectively. Data on the physicochemical and bacteriological parameters showed that the Buffalo River water quality was poor, and deteriorated in the plains compared to the upper reaches. These water quality data, the presence of enteric viruses and the QMRA data, that revealed unacceptably high risks of enteric virus infections, and of illness and mortality from the infections, show that the Buffalo River and its dams are contaminated waters that constitute significant public health hazards. Provision of adequate sanitary infrastructure will help prevent source water contamination, and public health education aimed at improving personal, household and community hygiene is imperative. Monitoring enteric viruses in rivers and source water dams is necessary and useful as a risk assessment tool for the exposed population. Future research should consider a comprehensive characterization of the detected viruses. This work is both a significant contribution to the molecular epidemiology of enteric viruses and the first report on molecular detection and quantification of enteric viruses in surface waters in the Eastern Cape. , Thesis (PhD) -- Faculty of Science and Agriculture, 2013
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The auto/biography of the liberation struggle and public representation of its memory and heritage :1994 to 2008
- Authors: Solani, Noel Lungile Zwelidumile
- Date: 2013-03
- Subjects: Monuments , Reconciliation - - Political aspects -- South Africa , Museums
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/23067 , vital:55380
- Description: This thesis is an analysis ofthe constructions and productions of new post apartheid identities and heritages of the liberation struggle with a specific reference on the Eastern Cape. It does this by examining the first 15 years ofdemocratic rule in South Africa. While there were many organisations that participated in the liberation struggle such as the African Peoples Organisation (APO). the lndian Congresses. the Pan Africanist Congress (PAC). Unity Movement as well as the African National Congress (ANC). the focus ofthis thesis is restricted to the ANC. In this case it examines what were the objectives and subjective reasons for the participation ofthe ANC in the liberation struggle. These questions are examined from the perspective ofthe ANC as one of the liberation movements that ultimately assumed power and had an opportunity to implement its vision and programmes in a new state. ln this way. this work shows that the objectives ofthe liberation struggle were aimed at replacing the apartheid racial order with a non-racial and non—sexist society where all its members are equal citizens. The thesis seeks to examine the feelings ofthe liberation struggle by looking at songs, poetry. photographs. documentary films. museums and monuments. I argue that, cultural practices and memory institutions such as museums. offers us useful complex methodologies in the narration ofthe auto/biography ofthe liberation struggle. I also argue that each of these does not only tell the narrative of the liberation struggle, trigger and promote the memory ofthat struggle and individuals within that struggle. but they also communicate the feelings ofthis struggle. its growth and maturity in ways that history as understood in the academy cannot The main question that is asked in this study is: what were the self—determined objectives ofthe National Democratic Struggle (NDS)? In this regard, I therefore examine two issues: first I look at the evolution ofthe national or native question in South Africa and the reaction or understanding ofthe liberation movements. especially the ANC. towards the national question. The thesis also examines the AN(“s attitude on this matter. Secondl). I attempt to analyse the “national question“ in a changed environment and the ways in which it is constructed through public memory and the means used for the "nation“ to where While not engaging on the merits or demerits of the national question. , Thesis (PhD) -- Faculty of Social Science and Humanities, 2013
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The changing face of NEPAD and the challenges of facilitating sub-regional economic integration on the ECOWAS platform
- Authors: Agomuonso, Udo Robertson
- Date: 2013-03
- Subjects: New Partnership for Africa's Development , Economic Community of West African States , Sustainable development -- Africa
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/24783 , vital:63573
- Description: This research is intended to evaluate and study the challenges confronting NEPAD’s quest for sub regional economic integration in the ECOWAS domain. Political and economic integration has been part of African strategy to overcome fragmentation, marginalization and improve the continent’s position in the global political economy. Indeed, Africa needs integration more than any other continent or region in the world and this is why, it has had a fair share of regional integration arrangements all through her history. Unfortunately, these efforts have not paid off in the dimension of desired expectations. When NEPAD was established, it was given regional integration responsibilities, even though it was not a regional organization. It was mandated to drive regional integration in Africa by supporting the regional integration efforts of the regional economic institutions in Africa. After 10 years of existence, African leaders dissolved NEPAD and in its place, instituted the NEPAD Planning and Coordinating Agency (NPCA). This move was orchestrated by the seemingly slow progress made by NEPAD in this direction. NEPAD and ECOWAS has been working together to promote regional integration, but like in most parts of Africa, the challenges has been seemingly intractable. The study is anchored around the principles of integration as presented by the neo-functionalists theorists. Hence, the research attempted to provide an explanation of the performance and non-performance of NEPAD as a regional integration tool, within the confines of the theory. The findings show that while, there has been some level of success in this venture as evidenced by the projects that are on-going in the sub region, the fact remains that NEPAD did not deliver or actually delivered below expectations. However, this situation is reversible as the study submitted that, there is a future for regional integration in West Africa, Africa and the new NPCA, if the African Union pulls the right levers. , Thesis (PhD) -- Faculty of Social Sciences and Humanities, 2013
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Characterization of bioflocculants produced by consortia of three marine bacteria belonging to the genera bacillus and cobetia previously isolated from the bottom sediment of Algoa Bay in the Eastern Cape Province of South Africa
- Authors: Ugbenyen, Anthony Moses https://orcid.org/0000-0002-1381-3428
- Date: 2013
- Subjects: Water -- Purification -- Flocculation , Water quality management , Flocculation
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/24454 , vital:62804
- Description: The bioflocculant-producing potentials of three marine bacteria isolated from the sediment samples of Algoa Bay in the Eastern Cape Province of South Africa were assessed. Analysis of the partial nucleotide sequence of the 16S rDNA of the bacteria revealed 99 percent, 99 percent, and 98 percent similarity to Cobetia sp. L222, Bacillus sp. A-5A, and Bacillus sp. HXG-C1 respectively and the sequence was deposited in GenBank as Cobetia sp. OAUIFE, Bacillus sp. MAYA and Bacillus sp. Gilbert (accession number JF799092, JF799093, and HQ537128 respectively). Cultivation condition studies for Cobetia sp. OAUIFE revealed that bioflocculant production was optimal with an inoculum size of 2 percent (v/v), initial pH of 6.0, Mn2+ as the metal ion, and glucose as the carbon source. Metal ions, including Na+, K+, Li+, Ca2+and Mg2+ stimulated bioflocculant production resulting in flocculating activity of above 90 percent. This crude bioflocculant is thermally stable, with about 78 percent of its flocculating activity remaining after heating at 100 oC for 25 min. Analysis of the purified bioflocculant revealed it to be an acidic extracellular polysaccharide. FTIR analysis revealed the presence of methoxyl, hydroxyl, and carboxyl - groups in the compound bioflocculant and SEM micrograph of the bioflocculant revealed a crystal-linear structure. On the other hand, bioflocculant production by Bacillus sp. MAYA was optimal when glucose (95.6 percent flocculating activity) and ammonium nitrate (83.3 percent flocculating activity) were used as carbon and nitrogen sources respectively; inoculum size was 2 percent (v/v); initial pH 6; and Ca2+ as coagulant aid. Chemical analysis of the purified bioflocculant shows that it is composed of uronic acid, neutral sugar and protein. FTIR analysis also revealed the presence of methoxyl, hydroxyl, carboxyl and amino- groups in this bioflocculant. The bioflocculant is thermostable with about 65.6 percent residual flocculating activity retained after heating the bioflocculant at 100 oC for 25 min. However bioflocculant production by Bacillus sp. Gilbert was optimal when sodium carbonate (95.2 percent flocculating activity) and potassium nitrate (76.6 percent flocculating activity) were used as carbon and nitrogen sources respectively; inoculum size was 3 percent (v/v); initial pH 9; and Al3+ as cation. The crude bioflocculant retained 44.2 percent residual flocculating activity after heating at 100 oC for 15 min. FTIR analysis reveals the presence of hydroxyl, carboxyl and methylene - groups in the compound bioflocculant. SEM micrograph of the bioflocculant revealed an amorphous compound. The consortia of these bacteria strains also produced bioflocculants with high flocculating activities which were highly efficient in removing turbidity and chemical oxygen demand (COD) from brewery wastewater, diary wastewater and river water. The bioflocculants from the consortia seemed better than traditional flocculants such as alum . The characteristics of the bioflocculant produced by the consortium of Cobetia sp. OAUIFE and Bacillus sp. MAYA showed that this extracellular bioflocculant, composed of 66percent uronic acid and 31percent protein and an optimum flocculation (90 percent) of kaolin suspension, when the dosage concentration was 0.8 mg/ml, under weak alkaline pH of 8, and Ca2+ as a coagulant aid. The bioflocculant is thermally stable, with a high residual flocculating activity of 86.7 percent, 89.3 percent and 87.0 percent after heating at 50 oC, 80 oC and 100 oC for 25 min respectively. The FTIR analysis of the bioflocculant indicated the presence of hydroxyl, amino, carbonyl and carboxyl functional groups. Scanning electron microscopy (SEM) image revealed a crystal-linear spongy-like bioflocculant structure and EDX analysis of the purified bioflocculant showed that the elemental composition in mass proportion of C,N,O,S and P was 6.67:6.23:37.55:0.38:4.42 (percent w/w). However, the characteristics of the bioflocculant produced by the consortium of Cobetia sp OAUIFE and Bacillus sp. Gilbert showed an optimum flocculation (90 percent) of kaolin suspension when the dosage concentration was 0.2 mg/ml, under neutral pH of 7, and Ca2+ as a coagulant aid. The FTIR analysis of the bioflocculant indicated the presence of hydroxyl and carbonyl functional groups. Scanning electron microscopy (SEM) image revealed an amorphous morphology. On the other hand the bioflocculant produced by the consortium of Bacillus sp. MAYA and Bacillus sp. Gilbert showed similar characteristic with the bioflocculant from the consortium of Cobetia sp. OAUIFE and Bacillus sp. Gilbert except for Al3+ being the preferred coagulant aid. The characteristics of the bioflocculant produced by the consortium of Cobetia sp. OAUIFE, Bacillus sp. MAYA and Bacillus sp. Gilbert showed an optimum flocculation (87 percent) of kaolin suspension when the dosage concentration was 1.0 mg/ml. Under strong alkaline pH of 12, flocculating activity reached (95 percent) when Al3+ was the coagulant aid. The FTIR analysis of the bioflocculant indicated the presence of hydroxyl, amino, carbonyl and carboxyl and phosphoryl functional groups. Scanning electron microscopy (SEM) image revealed a flaky amorphous morphological structure. Due to the excellent COD and turbidity removal efficiencies of the bioflocculants produced by the consortia, these make those attractive candidates for use in water and wastewater treatment. , Thesis (PhD) -- Faculty of Science and Agriculture, 2013
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Production and biochemical characterization of new bioflocculants from bacteria isolated from freshwater and marine environments of the Eastern Cape in South Africa
- Authors: Mabinya, Leonard Vuyani https://orcid.org/0000-0002-0682-7282
- Date: 2013-01
- Subjects: Flocculation , Bacteria
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/24228 , vital:62445
- Description: The production and characterization of bioflocculants produced by three bacteria belonging to Arthrobacter, Halomonas and Micrococcus genera and isolated from freshwater and marine environments were evaluated both as axenic cultures and as consortia. The influences of cultutre conditions such as carbon, nitrogen and metal ions sources, as well as initial pH on bioflocculant production by individual isolates were investigated. Both Arthrobacter sp. Raats and Halomonas sp. Okoh utilized urea as a nitrogen source of choice for optimal production of the bioflocculants with Micrococcus sp. Leo having a preference for peptone. All three strains differed in as far as the carbon source of choice was concerned with lactose, glucose and sucrose the preferred carbon sources respectively. Also, all three bacterial strains produced an extracellular bioflocculant aerobically but an intial pH 7.0 of the culture media was suitable for both Arthrobacter sp. Raats and Halomonas sp. Okoh with a slightly alkaline pH of 9.0 preferred by Micrococcus sp. Leo. The presence of Mg2+ cations stimulated bioflocculant production by both Arthrobacter sp. Raats and Micrococcus sp. Leo while Ca2+ resulted in more efficient bioflocculant production by Halomonas sp. Okoh. Chemical analyses revealed the bioflocculants produced by both Halomonas sp. Okoh and Micrococcus sp. Leo to be predominantly polysaccharides whereas Arthrobacter sp. Raats produced principally a glycoprotein composed of about 56percent protein and 25percent total carbohydrate. Response surface methodology (RSM) was used to optimize production medium for bioflocculant production by a consortium of Halomonas sp. Okoh and Micrococcus sp. Leo. Plackett-Burman experimental design showed that fructose, ammonium sulphate and MgCl2 were significant in the high yield of the bioflocculant. Furthermore, central composite design showed that optimal concentration of these critical nutritional sources were 16.14 g/L, 1.55 g/L and 1.88 g/L for fructose, ammonium sulphate and MgCl2 respectively. Quantification of the bioflocculant showed a yield of 6.43 g/L which was in close accord with the predicted value of 6.51 g/L. FTIR spectrometry of the bioflocculant indicated the presence of carboxyl, hydroxyl and amino groups, typical for heteropolysaccharide, while SEM imaging revealed a lattice-like structure. The efficiency of the nutrient optimization suggests suitability for industrial applicability. , Thesis (PhD) -- Faculty of Science and Agriculture, 2013
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Significance of accuracy in the orthographical development of isiXhosa in a post-democratic South Africa
- Authors: Saul, Zandisile W.
- Date: 2013-01
- Subjects: Xhosa language , Xhosa language -- Orthography and spelling , Xhosa language -- Grammar
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/23056 , vital:55325
- Description: IsiXhosa language is one of the first languages in sub-Saharan Africa to be reduced into writing. The first written texts in isiXhosa were produced by British missionaries in the Tyhume river valley as early as 1824. In spite of these early developments, there still remain today many inconsistencies and anomalies with regard to the standardization of isiXhosa orthography that require attention. This research focuses on the status quo of the current orthography of isiXhosa which is used in the writing of the selected texts which were published from 2000 to date. The research study investigates how these texts are written, that is, whether they are written according to the principles of the writing of this language as developed by the respective language boards or not. The reason for doing this is that this language has become one of the official languages of the Republic of South Africa. Therefore, in order for it to be read and written without any difficulty by anyone, as it is expected to be used as a medium of instruction even at tertiary level in the future, it should be written accurately and consistently. The study analyses a number of isiXhosa written texts including the following: (1) Selected literary texts which were published from 2000 to date. (2) Selected publications by government departments. (3) Selected translated texts. (4) Some of the newspapers and magazines which are currently in circulation in this language. (5) Some dictionaries of isiXhosa. (6) The updated isiXhosa Orthography as compiled by the PanSALB (2008). The aims of the study are outlined in Chapter 1. This chapter also provides the theoretical framework within which the research is located. Chapter 2 is about the theoretical perspectives on the development of the art of writing and orthography. The historical background, that is, the development of the art of writing and the general as well as the specific requirements of a good orthography are discussed. Chapters 3, 4 and 5 observe and analyse the orthographical features of some written documents of isiXhosa such as writing of capital letters, word division, usage of concords, etc. These three chapters also observe and analyse the various anomalies and inconsistencies in the spelling of isiXhosa words that were identified in the consulted texts. Chapter 6 is devoted to the conclusion and the recommendations. , Thesis (PhD) -- Faculty of Social Science and Humanities,2013
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Development & evaluation of modified lignocellulose-clinoptilolite composites for water treatment
- Authors: Vala, Mavula Kikwe Remy
- Date: 2012-12
- Subjects: Lignocellulose , Lignocellulose -- Biotechnology
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/24521 , vital:63051
- Description: Municipalities, mining, textile and many other industries release wastewater into water bodies. Thus, the entire ecosystem (biota and abiota) including drinking water is affected by polluted effluents. The growing environmental concern over water pollution (due to inorganic and persistent organic compounds) attracts a significant amount of research in the removal of pollutants from water. In this study, lignocellulose and clinoptilolite were modified for the preparation of composites, with high adsorption properties, suitable for the removal of pollutants. Grass (Kikuyu grass) material was first treated with boiling water in order to remove soluble compounds and then with sulfuric acid in order to free functional groups within lignocellulose. The lignocellulose obtained was then chemically modified with three different siloxanes (3-aminopropyl-terminated poly (di)methylsiloxanes) of different molecular weights. For clinoptilolite, impurities were removed by reflux in hydrochloric acid before chemical modification with siloxanes. Grafting of siloxanes onto lignocellulose and clinoptilolite as well as the preparation of composites were successfully achieved by means of dibutyltin dilaurate (catalyst) after reflux under nitrogen. The modified materials were characterized by FT-IR, XRD, SEM and TGA and results confirmed successful modification of the materials. Solid state 29Si and 13C NMR were used to investigate the nature of the composite prepared with siloxane NH40D (CNH40D). The investigation revealed a possible bond between the modified lignocellulose and the modified clinoptilolite in the composite. The sorptive and/or ion exchange properties of the materials prepared for the removal of pollutants from water were then investigated. Phenol red, used motor (engine) oil and cyanide were used (with regard to textile, oil spill and gold mining effluents respectively) to simulate water pollution in the laboratory. It was found that adsorption properties of lignocellulose were significantly increased after sulfuric acid treatment, suggesting the availability of lignocellulose functional groups as adsorption sites. When further modified with siloxanes, lignocellulose showed less efficiency in adsorbing phenol red. The general mechanism of phenol red uptake onto lignocellulose and clinoptilolite modified with siloxane or composites was: rapid initial adsorption, slow uptake, small rate increase and then equilibrium. The mechanism of phenol red uptake could be well represented by the pseudo second-order kinetic model with equilibrium being reached after a period of time, ranging between 1-5 hours. The linear Langmuir model was the best model for describing adsorption of phenol red onto lignocellulose modified with siloxanes and composites while the Freundlich model appeared to be best for clinoptilolite modified with siloxanes. The general mechanism of used motor oil uptake onto lignocellulose and clinoptilolite modified with siloxane or composites was: rapid uptake, equilibrium and the process occurs over a short period (10 min). The pseudo second-order kinetic model appeared to be the best representation of this adsorption. The linear Langmuir isotherms are the best fitted model for used motor oil uptake onto the adsorbents prepared. Adsorption of cyanide occurred very quickly (10 to 30 min). For lignocellulose and clinoptilolite modified with siloxanes, desorption occurred soon after adsorption and thus no kinetic model nor isotherms of adsorption were deduced. However, adsorption of cyanide onto composites could be represented by the pseudo second-order kinetic model. Nanofibres were fabricated by electrospinning of the modified lignocellulose and composites by blending them with PAN in a solvent mixture of DMF-DMSO. Nanofiltration was achieved by packing the nanofibres prepared into a pipette and filtering polluted water. Nanofiltration was assessed by measurement of the turbidity of water which dropped from 63 NTU for polluted water to 3.06 NTU for filtered water. , Thesis (PhD) -- Faculty of Science and Agriculture, 2012
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The implementation of HIV and AIDS prevention programmes in three Universities in Zimbabwe
- Authors: Muzenda, Denias https://orcid.org/0000-0002-6552-7319
- Date: 2012-12
- Subjects: Universities and colleges -- Health promotion services , AIDS (Disease) -- Prevention
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/25022 , vital:63891
- Description: Given the nature and devastating effects of HIV and AIDS and its impact on society, the universities in Zimbabwe have affirmed the need to define their institutional policy aimed at managing and mitigating the effects of HIV and AIDS. In this regard they have also put in place programmes to address the epidemic. Among the programmes are HIV and AIDS prevention programmes which are being implemented. However, there have been concerns that despite the fact that the programmes are being implemented as planned they have not met their objectives as HIV and AIDS infections have not decreased as expected. Hence this study assesses the implementation of HIV and AIDS prevention programmes in three universities in Zimbabwe. To achieve these objectives, the researcher opted for the mixed method methodology which is embedded in the post-positivist research paradigm. Post-positivist research paradigm attempts to increase our understanding of the way things are and that objectivity is an ideal that can never be achieved, and research is conducted with greater awareness of subjectivity. The researcher had to opt for questionnaires, interviews, document analysis and observations as data collecting instruments. From the Data presented the study found that universities lack adequately professionally qualified HIV and AIDS lecturers. Results on capacity of implementers in the study revealed that an insignificant number of lecturers have the capacity while the majority significant number lacked the capacity to implement the programmes. HIV and AIDS prevention is being implemented by unqualified lecturers who lack training and prerequisite skills for the programme. Furthermore, the study found that Lecturers do not get the necessary professional and institutional support to implement successfully HIV and AIDS programmes in universities. HIV and AIDS Coordinators and NGOs expressed concern over the inadequacy of learning and teaching support material. Funding and lack of resources such as specialist rooms and learning materials are a major impediment to successful implementation of HIV and AIDS. Implementation of HIV and AIDS programmes suffers from inadequate supervision by the relevant stakeholders due to unavailability of transport and financial cost. It also emerged that Human resource is also a major problem since there is a great staff turnover as specialist lecturers leave the system for better paid opportunities in the private sector. Most university lecturer respondents cited staff turnover as long-established and skilled lecturers look for greener pastures within and outside the country. The dominant methods used to implement HIV and AIDS programmes do not give enough room for group work discussions to effect behaviour change in students. Rather, more emphasis is on factual knowledge for assignments and examination purposes. On monitoring and evaluation of HIV and AIDS programmes, the study found that lecturers and students are unaware of their programmes progress bench marks. Universities have not been carrying out internal audits over the past years. This implies that the universities are divorced from Ministry of Higher Education Policy on monitoring and evaluation. Also, less people are involved in the monitoring process of HIV and AIDS programmes. The study found from the universities records that an HIV and AIDS Policy document exists at each university. However, the implementation of HIV and AIDS prevention programmes seemed to have a number of controversies and challenges. The study made the following recommendations against each major finding: Within the organisation, university works continually to equip staff with the knowledge, skills and tools required to analyse programme, implement and monitor interventions for HIV prevention. Capacity to support innovations includes aspects which hinder or support the implementation of new practices and ideas in the new curriculum. Universities should have trained HIV and AIDS Peer educators. For a lecturer to be considered as professionally qualified personnel in universities, the Ministry of Higher and Tertiary Education should stipulate possession of a Master‟s degree or a Doctorate. The researcher provided a section on contribution of the study to new knowledge whereby the researcher proposed an alternative model for HIV and AIDS implementation for universities in Zimbabwe. This model was derived after a thorough analysis of the findings from the study as well as an extensive literature review on the subject. Areas for further research have been suggested in this study. , Thesis (PhD) -- Faculty of Education, 2012
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Biological activities and mechanisms of action of two ethnobotanically selected South African medicinal plants on some bacteria associated with gastrointestinal infections
- Authors: Olajuyigbe, Olufunmiso Olusola https://orcid.org/0000-0002-7889-0416
- Date: 2012-08
- Subjects: Medicinal plants , Herbs -- Therapeutic use , Gastrointestinal system
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10353/25439 , vital:64249
- Description: In this study, 36 plant species representing 24 families were found to be commonly used for the treatment of a variety of gastrointestinal disorders in Eastern Cape, South Africa. The family Fabaceae had the highest number of species. Out of these, 47.06percent were used in the treatment of dysentery alone while 46.15percent were used in the treatment of diarrhoea. Acacia mearnsii De Wild and Ziziphus mucronata subsp. mucronata Willd were selected for this research because they are extensively used in folkloric medicine in South Africa and there was lack of scientific reports that documented their biological activities. The phytochemical screening, antioxidant activities, in vitro antimicrobial activities, cytotoxicity, the synergistic potentials and mechanisms of actions of these plants were investigated. The phytochemical screening and the antioxidant activities of the two species showed that the quantity of the phenolic compounds, flavonoids and proanthocyanidins detected differ significantly in the various extracts. Of the aqueous, acetone, ethanolic and methanolic extracts of A. mearnsii, the ethanolic extract had the highest flavonoids while the acetone extract had the highest phenolic contents. The proanthocyanidins were highest in the methanol extract while aqueous extracts had the least phytochemicals. Aqueous extract showed the least ferric reducing power but methanol extract indicated the highest reducing power. The reducing power of the extracts was lower than those obtained from the reference standard such as butylated hydroxytoluene (BHT), rutin and ascorbic acid. 2,2’-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) diammonium salt showed that ethanol extract exhibited the highest antioxidant activity at the highest concentration tested. Also, 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay indicated that ethanol extract had the highest radical scavenging activity at the lowest concentration and the activities of all the extracts decreased with increase in their concentrations. In Z. mucronata subsp. mucronata, the phenolics were significantly higher than the flavonoids and proanthocyanidin contents in all the extracts investigated. The ethanol extract had the highest antioxidant activity, followed by the acetone extract while the aqueous extract was the least active. Reacting with ABTS, the 50percent inhibitory concentrations (IC50) were (0.0429 ± 0.04 mg/ml) for aqueous, (0.0317 ± 0.04 mg/ml) for acetone and (0.0306 ± 0.04 mg/ml) for ethanol extracts while they inhibited DPPH radical with 50percent inhibitory concentration (IC50) values of 0.0646 ± 0.02 mg/ml (aqueous), 0.0482 ± 0.02 mg/ml (acetone) and 0.0422 ± 0.03 mg/ml (ethanol). The investigation showed that a positive linear correlation existed between the total phenolic content and antioxidant activity of the extracts and that these plants have strong antioxidant property and free radical scavenging capability. The in vitro antibacterial activities of Acacia mearnsii and Z. mucronata subsp. mucronata showed that their minimum inhibitory concentrations ranged between 0.039 mg/ml and 1.25 mg/ml. With the exception of acetone extract of A. mearnsii having MICs greater than 1.0 mg/ml for Enterococcus faecalis ATCC 29212 and Bacillus subtilis KZN, all other isolates had MICs less than 0.7 mg/ml. In all the bacteria treated with Z. mucronata subsp. mucronata extracts, Enterobacter cloacae ATCC 13047 had MIC greater than 1 mg/ml in methanol extract, Enterococcus faecalis ATCC 29212 and Staphylococcus aureus ATCC 6538 had MICs greater than 1 mg/ml in acetone extract while all other isolates were highly susceptible to the different extracts of Z. mucronata subsp. mucronata and had MICs less than 0.7 mg/ml. While aqueous extract was as active as the alcoholic extracts in A. mearnsii, that of Z. mucronata had no effect. The ethanol extracts exhibited the highest degree of antibacterial activity in both plants. This study, also, showed that the antifungal activity of A. mearnsii ranging 0.3125 – 5.0 mg/ml was higher than those of the different extracts of Z. mucronata subsp. mucronata ranging 1.25 – 10.0 mg/ml. It is evident from the results of the brine shrimp lethality assay that the crude extracts of A. mearnsii with the LC50 equaled 112.36 µg/ml and having the highest levels of toxicity (100percent) death at 500 μg/ml was non toxic (LC50 > 100 μg/ml) while the LC50 for Z. mucronata subsp. mucronata equaled 90.27 µg/ml indicated a low level of toxicity. The effects of combining the crude extracts of these plants with eight antibiotics were investigated by means of checkerboard and agar diffusion methods. On using the methanol extract of A. mearnsii, the agar diffusion assay showed that extract-kanamycin combination had zones of inhibition ≥ 20 ± 1.0 mm in all the bacteria tested (100percent), followed by extract chloramphenicol (90percent) > extract-ciprofloxacin = extract-tetracycline (70percent) > extract amoxicillin (60percent) > extract-nalidixic acid (50percent) > extract-erythromycin (40percent) > extract metronidazole (20percent). The checkerboard showed synergistic interaction (61.25percent), additivity/indifference (23.75percent) and antagonistic (15percent) effects. I, therefore, concluded that the antibacterial potentials of the antibiotics were improved and combining natural products with antibiotic could be a potential source of resistance-modifying agents useful against multi-drug resistant bacteria. The influences of these extracts on the ultrastructures, elemental components, protein and lipid leakages of five different bacteria were determined as the possible mechanisms of action of the extracts investigated. The scanning electron microscopy indicated varied ultrastructural changes in the morphology of bacterial cells treated with the extracts. The X-ray microanalysis showed significant differences between the elemental contents of extract-treated and untreated bacteria while lipids and proteins were leaked to a great extent from the extract-treated bacterial strains in comparison with the untreated ones. The possible mechanisms of action of the extracts may include inhibition of a significant step in peptidoglycan assembly, inhibition of metabolic processes, disruption of cell wall and cell membranes resulting in the efflux of lipid and protein in all the bacteria tested. The possible mechanism of action involved in the lipid and protein leakages in the bacterial cells could be attributed to lipid peroxidation and protein oxidation owing to the antioxidant activities of the extracts that were active beyond the protective levels. I concluded that the morphological changes and the observed leakages showed rapid killing, significant membrane depolarization resulting in leakages and efflux of disintegrated cellular materials. In general, this study has justified the ethnotherapeutic importance of A. mearnsii and Z. mucronata subsp. mucronata in the treatment of microbial infections by indicating the possible mechanisms of action of the crude extracts on the tested bacteria. , Thesis (PhD) -- Faculty of Science and Agriculture, 2012
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