Culturally-relevant augmented user interfaces for illiterate and semi-literate users
- Authors: Gavaza, Takayedzwa
- Date: 2012 , 2012-06-14
- Subjects: User interfaces (Computer systems) -- Research , Computer software -- Research , Graphical user interfaces (Computer systems) -- Research , Human-computer interaction , Computers and literacy
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4665 , http://hdl.handle.net/10962/d1006679 , User interfaces (Computer systems) -- Research , Computer software -- Research , Graphical user interfaces (Computer systems) -- Research , Human-computer interaction , Computers and literacy
- Description: This thesis discusses guidelines for developers of Augmented User Interfaces that can be used by illiterate and semi-literate users. To discover how illiterate and semi-literate users intuitively understand interaction with a computer, a series of Wizard of Oz experiments were conducted. In the first Wizard of Oz study, users were presented with a standard desktop computer, fitted with a number of input devices to determine how they assume interaction should occur. This study found that the users preferred the use of speech and gestures which mirrored findings from other researchers. The study also found that users struggled to understand the tab metaphor which is used frequently in applications. From these findings, a localised culturally-relevant tab interface was developed to determine the feasibility of localised Graphical User Interface components. A second study was undertaken to compare the localised tab interface with the traditional tabbed interface. This study collected both quantitative and qualitative data from the participants. It found that users could interact with a localised tabbed interface faster and more accurately than with the traditional counterparts. More importantly, users stated that they intuitively understood the localised interface component, whereas they did not understand the traditional tab metaphor. These user studies have shown that the use of self-explanatory animations, video feedback, localised tabbed interface metaphors and voice output have a positive impact on enabling illiterate and semi-literate users to access information. , TeX , Adobe Acrobat 9.51 Paper Capture Plug-in
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- Date Issued: 2012
Curcumin analogues as ligands for Re (I) and (V)
- Authors: Schmitt, Bonell
- Date: 2012
- Subjects: Rhenium , Ligands , Chemistry, Inorganic
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10443 , http://hdl.handle.net/10948/d1020975
- Description: Coordination properties of 4-bromo-N-(diethylcarbamothioyl)benzamide (Hbeb) and 4-bromo-N-(diphenylcarbamothioyl)benzamide (Hbpb) with oxorhenium(V) and rhenium(I) are reported and discussed. Transition metal complexes of these ligands were studied due to the wide range of applications of thiourea derivatives in biological fields. N-[Di(alkyl/aryl)carbamothioyl]benzamide derivatives readily coordinate to metal ions as O,S-donors and the catalytic property of the complexes can be altered by these ligands, due to steric and electronic properties provided by various substituents. The coordination possibilities of curcumin with rhenium(V) are discussed, as well as the difficulties encountered. Analogues of curcumin have been made, which also contains a seven-spacer unit between the phenyl rings, which would be more reactive and more effective in bonding to rhenium and which have greater or a similar biological activity to curcumin. This was done by assessing the coordination properties of 1,5-bis(salicylidene)thiocarbohydrazide (H4salt) and 2,4-bis(vanilidene)thiocarbohydrazide (H4vant) with oxorhenium(V) and rhenium(I) starting materials. Two rhenium(V) complex salts of the core [ReX(PPh3)2]4+ (X = Br, I), containing a coordinated imido nitrogen, are reported. One is a ‘2+1’ complex, coordinating bi- and monodentately, with the other a similar ‘3+0’ complex containing a tridentate imido-coordinated Schiff base. Selected compounds were tested against oesophageal cancer cell lines in order to evaluate and compare their effectiveness in eliminating or reducing the cancer cells in the test medium during biological testing.
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- Date Issued: 2012
Defect in the South African construction industry
- Authors: Le Roux, Martyn
- Date: 2012
- Subjects: Construction industry -- South Africa , Buildings -- Defects -- South Africa , Quality assurance -- Standards -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:9711 , http://hdl.handle.net/10948/d1020320
- Description: Lack of quality in the South African construction industry is causing various problems for different stakeholders. The causes of this lack in quality are due to various different attributes. Corruption has become one of the leading causes with regards to lack of quality. Quality assurance can mitigate or eliminate defects through the implementation of a quality management system. Defects manifest primarily through cracking, dampness, detachment, and water leaks. Defects are categorized as structural, subsidence, acoustic and thermal. In terms of time, defects are either patent or latent, and can be discovered through observation, inspection and various tests. The causes of defects are ultimately due to error or omission, either during design or during the construction phases. Defects may also result due to procurement related factors, such as appointment of incompetent contractors. The result of defects is customer dissatisfaction, rework and disputes. Non-conformance to requirements results in rework and this in turn contributes to time and cost overruns. The aim of this research was to investigate the greatest cause that leads to defects in houses; the most common type of defect; and why projects fail in term of project management terms (due to defects). Results of quantitative research amongst professionals within the construction industry in the Western and Eastern Cape of South Africa as well as literature that has been reviewed form the basis of this study. Architectural practices, consulting engineering practices, and general building contractors were selected on a random sample basis, and surveyed using an online questionnaire. The study revealed that inadequate artisan skills is the biggest cause leading to defects in houses, and that cracks are the most frequent type of defect occurring. Projects fail in project management terms because of defects as the construction time of the projects are increased. The study revealed that construction related causes of defects dominate over design related causes. This study should be of value to both construction industry professionals as well as their clients.
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- Date Issued: 2012
Design and evaluation of illustrated information leaflets as an educational tool for low-literate asthma patients
- Authors: Wrench, Wendy Merle
- Date: 2012 , 2012-10-08
- Subjects: Asthma -- South Africa -- Study and teaching , Asthmatics -- South Africa -- Education
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:3867 , http://hdl.handle.net/10962/d1016236
- Description: Asthma is a chronic non-communicable disease associated with an increase in morbidity, mortality and economic burden. Globally 300 million people have asthma and it is estimated that one in every 250 deaths worldwide are due to asthma. South Africa has the highest asthma prevalence (8.1%) in Africa and the disease is 18th in the top 20 causes of death. Inadequate home management, poor availability of health care, and poor transport and emergency services are recognised as important contributing factors. Patients with a low level of education and limited literacy skills may be unable to understand instructions on frequency and use of asthma medicines, which could result in unintentional non-adherence leading to serious complications and increased health care costs. The aim of this study was to investigate the impact of a tailored educational intervention on low-literate patients with asthma. Objectives to achieve this aim included designing patient information leaflets (PILs) containing information on asthma, management of asthma and asthma therapy, and using the PILs to educate low-literate asthma patients. A before-andafter intervention type design evaluated self-reported selected health-related quality of life measures, self-reported self-efficacy, knowledge of asthma and asthma management, knowledge of the use of metered dose inhalers (MDIs) and MDI technique. The acceptability and understanding of the tailored PILs was also investigated. Two simple, readable PILs containing pictograms were developed in English and then translated into isiXhosa, the home language of the majority of the target population. Various guidelines on the design of health-related information for people with low-literacy were consulted and input on the design was received from health care providers, patients and graphic artists. A pilot study was conducted at a local primary health care (PHC) clinic to evaluate the PILs and final modifications to the PILs were made based on feedback received. For the main study, patients were recruited from the KwaNonqubela PHC clinic in Alexandria in the Eastern Cape, South Africa. Patients were 18 years or older, dependent on public sector health care facilities, diagnosed with asthma, prescribed a MDI (beclomethasone and/or salbutamol) for at least one month and English or isiXhosa-speaking. The exclusion criterion for patients in this study was involvement in any other asthma educational intervention during the period of study. Interviewer-led structured questionnaires were administered to 55 patients at the baseline and follow-up. Data collected include demographics, brief medical history and current asthma medications. Self-efficacy and iii health-related quality of life were assessed. Knowledge of asthma and asthma management was evaluated, and the use of beclomethasone and/or salbutamol metered dose inhalers was assessed. The PIL ‘Understanding asthma and trigger factors of asthma’ formed part of the educational intervention to explain asthma and aspects related to its management. Inhaler technique was evaluated and corrected using the PIL ‘How to use your pump’ together with a demonstration of correct technique by the investigator. Follow-up interviews were conducted approximately four weeks after baseline. PIL acceptability, readability and understanding of each pictogram were investigated at follow-up only. The educational intervention resulted in a significant increase in mean knowledge of asthma from 52.7% at baseline to 75.5% at follow-up. Gender was not associated with knowledge, but there was a significant age effect at baseline only, with the younger patients achieving better knowledge results. In both phases, patients with higher education had improved scores. A significant increase (2.4% to 38.6%) in the number of patients taking the minimum recommended adult dose of beclomethasone was noted but it is a matter of concern that the majority of patients were taking less than this. Patient self-reports suggested a significant increase in adherence, with the number of patients taking beclomethasone daily increasing from 33.3% to 61.3%. Self-reported management and control of asthma improved and this was reflected by the enhanced HRQOL results. MDI technique also improved significantly with an increase in the mean number of correct steps from 4.6 ± 2.2 to 7.9 ± 2.7. Education had a significant effect on MDI technique with more errors associated with lower educational status. There were no significant age or gender effects on the total number of correct steps in either phase. The illustrated PILs were received favourably with the majority of literate patients reporting that they were easy to read. Patients commented positively on the inclusion of pictograms and stated that the pictograms had served as aids in the understanding of asthma, trigger factors of asthma and correct MDI technique. The results of this study show that specially designed illustrated PILs can be an effective tool in educating low-literate patients with asthma. , Adobe Acrobat Pro 11.0.0 Paper Capture Plug-in
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- Date Issued: 2012
Developing a form-process framework to describe the functioning of semi-arid alluvial fans in the Baviaanskloof Valley, South Africa
- Authors: Bobbins, Kerry Leigh
- Date: 2012
- Subjects: Alluvial fans -- Research -- South Africa Sedimentation and deposition -- Research -- South Africa Geomorphology -- Research -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4825 , http://hdl.handle.net/10962/d1005500
- Description: The Baviaanskloof catchment is a semi-arid catchment located in the Cape Fold Mountains of South Africa. Little is known about the functioning of the complicated Baviaanskloof fluvial system and the role alluvial fans in the fluvial landscape. This thesis will contribute to field of geomorphology and, more specifically, to the field of fan morphometry by producing a standalone fan framework outlining methods to investigate the influence of external and internal control variables on alluvial fans. In this thesis, outcomes of the applied framework and case study are used to develop fan restoration guidelines for the Baviaanskloof Valley. The framework incorporates external and internal fan control variables at a valley-wide and local fan scale. External control variables include accommodation space, base-level change, and drainage basin inputs. Internal control variables include fan style, morphometry and fan channels. In order to apply the framework, fan morphometry data was required. This data was collected by creating a spatial plan of fans and basins in the valley. Outcomes of the applied framework include; an understanding of baselevel change on fans, relationships between fan basin characteristics and the fan surface and insight into fan channel processes. Results of the applied framework are investigated further using bivariate (correlation matrix) and multivariate (principle component analysis and regression analysis) analysis techniques. Significant relationships identified are: drainage basin area versus fan area, fan area and fan slope and drainage basin ruggedness and basin size. The primary outcomes of this thesis include an alluvial fan form-process framework, key considerations to be included in alluvial fan restoration projects and fan restoration guidelines. Contributions of this thesis to broader alluvial fan morphology science includes new insights into general fan literature by compiling a form-process alluvial fan classification framework to identify external and internal fan control variables and identify fan form. Additions have been made to Clarke’s (2010) evolutionary stages to describe stages 4 and 5 of fan evolution that has been adapted to describe fan evolution and differentiate between stages of mature fan evolution. This thesis has also contributed to the study of alluvial fans in South Africa, particularly in the Baviaanskloof Valley. The layout of the procedural guidelines and key considerations for an alluvial fan project provides a guide for rapid fan assessment for maximum cost and time benefits for stakeholders.
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- Date Issued: 2012
Development, assessment and optimisation of oral famciclovir formulations for paediatric use
- Authors: Magnus, Laura
- Date: 2012
- Subjects: Drugs -- Dosage forms , Drugs -- Analysis , Capsules (Pharmacy) , Antiviral agents , Pediatrics
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:3870 , http://hdl.handle.net/10962/d1018244
- Description: Many Active Pharmaceutical Ingredients (API) such as the antiviral agent famciclovir (FCV) are required for paediatric treatment but are not commercially available in age-appropriate dosage forms. It is common practice to prepare oral liquid dosage forms using commercially available tablets, capsules or powdered API and then dispersing or dissolving the crushed and/or powdered materials in a vehicle that the patient can swallow. Vehicles that are commonly used for this purpose include methylcellulose, syrup or combinations of these carriers where possible or commercially available suspending agents such as Ora-Sweet®, if available, can be used. However, several critical factors are overlooked when manufacturing extemporaneous formulations including, but not limited to, physical and chemical properties of the API, excipients, compatibility, stability and bioavailability issues. A stability-indicating High Performance Liquid Chromatography (HPLC) method for the analysis of FCV was developed and validated according to the International Conference on Harmonization (ICH) guidelines. The method is sensitive, selective, precise, accurate and linear over the concentration range 2-120 μg/ml. The stability of 25 mg/ml FCV formulations was assessed in vehicles manufactured from syrup simplex, hydroxypropyl methylcellulose (HPMC), Ora-Sweet® and an aqueous buffer (pH 6) following storage at 25 °C/60% RH and 40 °C/75% RH over six (6) to eight (8) weeks. The shelf life of the products was calculated as the longest period of storage for approximately 90% of the added FCV to be recovered. Formulations were manufactured using syrup simplex or HPMC with methylparaben and propylparaben individually or in combination and with sodium metabisulphite, ascorbic acid or citric acid as antioxidants. The resultant products were subject to quality control analysis for API content, viscosity, pH and appearance and the resultant data were subject to statistical analysis. The degradation rates were calculated for each product and a degradation profile plotted. The degradation rates of FCV in extemporaneous formulations were compared to those of FCV manufactured using a commercially available suspending agent and a buffered vehicle. FCV undergoes major degradation in the presence of sucrose, as observed for formulations in which the vehicle was syrup and Ora-Sweet®. FCV was found to be most stable when dissolved/dispersed in an HPMC vehicle incorporating sodium metabisulphite and a combination of parabens. The formulation that exhibited the maximum stability was manufactured using an aqueous solution buffered to pH 6. Due to the enhanced stability of FCV when added to a buffered vehicle a formulation in which an HPMC vehicle buffered to pH 6 with sodium metabisulphite, methylparaben and propylparaben was selected for optimisation using a Central Composite Design approach (CCD). In this way it was possible to establish a relationship between input variables such as pH, % w/v HPMC, % w/v antioxidant and % w/v preservative and the responses selected for monitoring by means of response surface modelling. A quadratic model was found to be the most appropriate to describe the relationship between input and output variables. Thirty batches of product were randomly manufactured according to the CCD and analysed to establish the stability in respect of viscosity, pH and the amount of FCV remaining following storage and the data were fitted to models using Design-Expert® software. A correlation between input variables and the responses was best described by a quadratic polynomial model. Analysis of Variance indicated that the response surface models were significant (P-value < 0.0001). The pH to which a FCV formulation was buffered was the most significant factor to effect the % drug content and the ultimate pH of the formulation, while the % w/v HPMC had the most significant effect on the viscosity of the product. The optimum composition for the manufacture of an oral liquid FCV formulation was predicted using the optimisation function of the Design-Expert® software. A low % error of prediction was established, indicating that the model is robust and that RSM is an appropriate formulation optimisation tool as it has a high prognostic ability. A liquid FCV formulation was developed, optimised and found to be suitable for its intended purpose. However further optimisation is required in respect of colourants, sweeteners and/or flavourants. The approach followed is useful in ensuring the development of quality products and can be applied in future.
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- Date Issued: 2012
Enumeration of insect viruses using microscopic and molecular analyses: South African isolate of cryotophlebia leucotreta granulovirus as a case study
- Authors: Dhladhla, Busisiwe I R
- Date: 2012
- Subjects: Baculoviruses , Insects -- Viruses , Molecular genetics , Microbial genomics
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10318 , http://hdl.handle.net/10948/d1008395 , Baculoviruses , Insects -- Viruses , Molecular genetics , Microbial genomics
- Description: Baculoviruses have been used as biocontrol agents to control insect pests in agriculture since the 1970s. Out of the fifteen virus families known to infect insects, baculoviruses offer the greatest potential as insect biopesticides, due to their high host specificity which makes them extremely safe to humans, other vertebrates, plants and non-target microorganisms. They comprise of two genera: nucleopolyhedroviruses (NPVs) and granuloviruses (GVs). The South African isolate of Cryptophlebia leucotreta granulovirus (CrleGV-SA) which is infectious for the false codling moth (FCM), Thaumatotibia leucotreta, (Meyrick) (Lepidoptera: Tortricidae), has been successfully developed into two commercial biopesticides; Cryptogran® and Cryptex®, for the control of FCM in citrus crops. The current method of enumeration used for CrleGV-SA virus particles in routine experiments during the production of the GV as biopesticides, is dark field microscopy. However, due to the small size of GVs (300-500 nm in length), the technique is not easy to perform on these viruses, and no systemic comparison has been made of potential alternative methods. Therefore, the main objective of this study was to develop a quantitative enumeration method for CrleGV-SA occlusion bodies (OBs) which is accurate, reliable, and feasible, and compare the developed methods of enumeration to the current method. Purified and semi-purified CrleGV-SA viral stocks were prepared for enumeration studies using spectrophotometry, dark field microscopy, scanning electron microscopy (SEM) and real time qPCR. Spectrophotometry was found to be an unreliable method for enumeration of GVs in the production, standardisation, and quality control of biopesticides. Dark field microscopy and SEM were found to be accurate, and statistically comparable (p = 0.064) enumeration techniques. qPCR is currently being optimised for the enumeration of GVs. This technique was demonstrated to generate accurate standard curves for absolute quantification of virus particles for pure and semi-pure virus preparations. qPCR offers the greatest potential as an accurate enumeration method because it is not affected by contamination with non-biological contaminating debris, nor by other biological material due to the specificity of PCR primers. Further work is required to fully develop qPCR as an enumeration method for GVs. However, dark field microscopy has been successfully validated as an enumeration method. SEM, which has a high resolution compared to light microscopy, has an added advantage over dark field microscopy, which is to distinguish virus particles in semi-pure viral stock preparations during counting. Therefore, SEM currently provides the most unambiguous and feasible enumeration method for GVs in both purified and semi-purified virus samples.
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- Date Issued: 2012
Environmental cues and sensory preferences directing the nesting process in loggerhead turtles, caretta caretta, nesting in Maputaland, South Africa
- Authors: Brazier, Wayne
- Date: 2012
- Subjects: Sea turtles
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10721 , http://hdl.handle.net/10948/d1015688
- Description: Animals use their senses for everything on an immediate, and day to day fashion – detecting danger, finding food, finding mates among other activities. In sea turtles and other migratory species senses are used for long-distance migrations. Senses such as smell, vision and hearing, have been studied experimentally under laboratory conditions but seldom have been investigated in the field. This thesis takes a combination of field and laboratory experiments and investigates some of the hypotheses involved in natal homing and nest site selection. The loggerhead’s nesting distribution in Maputaland is distinct with characteristic high and low density nesting areas which are consistent from year to year. Investigations by earlier researchers on these beaches suggested that beach characteristics, such as beach morphodynamic type and beach width, do not appear to influence the beaches at which loggerheads emerge to nest. The high density nesting area (with approximately 440 loggerhead emergences/km) have similar beach characteristics as the low density nesting area (with <50 loggerhead emergences/km). It is therefore suggested that there is another cue that drives nest site selection. It can either be related to a physical characteristic not yet realised, or is a non-physical (but chemical or biological) cue. This dissertation aims to identify the sensory inputs received during the nest selection process, as well as sea-finding ability after nesting. To investigate the mechanism causing the high-density as opposed to the low-density nesting area, three potential drivers were investigated namely: chemical imprinting (as a natal beach cue), ambient and artificial light (as deterrents) and social facilitation (as a learned behaviour). It was also attempted to identify the strength of the most common senses – vision, hearing and smell. As animal ethics restricts interfering with emerging or nesting turtles, the strengths of these senses were tested during sea-finding by adult loggerheads. The results indicate that sulfide concentrations appear to be used as chemical cues for nesting as these concentrations are elevated (>150 percent) in the high density nesting beaches compared to the low density nesting beaches within and among seasons however further investigations are required. Artificial light (range: 0.045–0.5 lux) is an active deterrent of female emergences while ambient light, even under extreme conditions such as lightning during electric storms (up to 8.2 lux), appears to have no observable influence on the spatial or temporal distributions of emergences. Social facilitation appears unlikely as a primary nest site selection factor for loggerheads. It may however, play a minor secondary role to preferred areas or hotspots. Sea-finding in post-nesting female loggerheads appears to be driven exclusively by visual cues such as the light horizon, with minimal to no influence from other cues (the sound of the breakers, slope or smell of the ocean) which solidify the visual system’s use in sea-finding. This research on the nest site selection of loggerheads and the sensory systems involved in this process has added valuable information to the limited pool of knowledge already present and has created a solid framework on which further investigations can be based. Future work in this field should focus on integrating a suite of sensory stimuli and cues to receive a greater understanding of the sensory systems used in nest site selection.
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- Date Issued: 2012
Evaluation for harnessing low-enthalpy geothermal energy in South Africa based on a model pilot plant in the Limpopo Mobile Belt
- Authors: Dhansay, Taufeeq
- Date: 2012
- Subjects: Geothermal resources -- South Africa -- Limpopo , Renewable energy sources -- South Africa -- Limpopo , Climatic changes -- South Africa -- Limpopo
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10667 , http://hdl.handle.net/10948/d1019789
- Description: South Africa generates more than 90 percent of its total energy capacity through non-renewable sources. With coal forming the predominant energy source, South Africa became the leading carbon emissive nation in Africa, emitting 450 million tonnes of CO2 in 2011. In an international effort to restrict global average temperature rise to 2° C above the average prior the industrial revolution, the Kyoto Protocol has been extended for another 8-year commitment period. This is complementary to an expected resolution of a new legally binding climate change policy in 2015. This policy will aim to introduce financial penalties for nations failing to meet ascribed GHG emission targets by 2020. In an attempt to meet these climate change resolutions South Africa will research and develop cleaner, alternative forms of energy, including hydro, wind, and biomass forms of renewable energy, in addition to designating stringent building regulations for the Incorporation of solar energy. These measures form part of an Integrated evelopment Plan that aims to generate a target of 10,000 GWh of renewable energy in 2013. South Africa is also investigating the possibilities of extracting its shale gas reserves and implementing it as a major energy source. This energy mix has given little attention to geothermal energy. The reasons for this omission appears to be the lack of active volcanism and previous research that suggests South Africa, largely underlain by the Kaapvaal Craton, has a relatively low heat Flow profile, deemed insufficient for harnessing geothermal energy.
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- Date Issued: 2012
Evaluation of noise levels of micro-wind turbines using a randomised experiment
- Authors: Clohessy, Chantelle May
- Date: 2012
- Subjects: Wind turbines -- Noise , Wind turbines -- valuation
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10508 , http://hdl.handle.net/10948/d1015974
- Description: One of the biggest environmental concerns of a wind turbine is the wind turbine noise (Prospathopoulos and Voutsinas, 2007). This study assesses the noise impacts of wind turbines on the environment by comparing the micro-wind turbine noise to traditional accepted surrounding sounds. The collection of the sound level data was done by using a randomised experiment. The sound level data was then fitted to a General Linear Model to determine the relationship between the sound levels generated at a given site to the time of day, wind speed, wind direction and distance from the sound source. An additional study was conducted to determine the relationship between wind speed and the sound levels of wind turbines. The distribution of frequency components of wind turbine sound was also determined.
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- Date Issued: 2012
Factors affecting survivorship of loggerhead (Caretta caretta) and leatherback (Dermochelys coriacea) sea turtles of South Africa
- Authors: De Wet, Anje
- Date: 2012
- Subjects: Loggerhead turtle -- South Africa , Leatherback turtle -- South Africa , Sea turtles -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10686 , http://hdl.handle.net/10948/d1007900 , Loggerhead turtle -- South Africa , Leatherback turtle -- South Africa , Sea turtles -- South Africa
- Description: Loggerhead (Caretta caretta) and leatherback (Dermochelys coriacea) sea turtles as well as their eggs and hatchlings have been protected on their nesting beach in South Africa (SA) since 1963. Both nesting populations were expected to show similar trends in recovery following the application of identical protection and conservation measures. The loggerhead nesting population has responded favourably to these protection efforts. In contrast, the leatherback nesting population showed an initial increase but is currently stable. The reason for this difference in response is thought to be due to differential offshore mortality of these two species. This prompted an investigation into the different sources of sea turtle mortality in the South Western Indian Ocean (SWIO). Specific aims were to identify and quantify sources of loggerhead and leatherback mortality on nesting beaches as well as in the oceans. Reasonable survivorship at all age classes is important to ensure recruitment of new nesting individuals into sea turtle populations. Mortality of nests, eggs per nest and hatchlings were quantified over two seasons for the loggerheads and leatherbacks nesting in SA. The beach was patrolled on foot to encounter and record females emerging from the ocean and later, hatchlings from their nests. The nests were then monitored during the incubation period and excavated once hatched. The fates of 925 nests were determined during these two nesting seasons (2009/2010 and 2010/2011). The main source of loggerhead and leatherback nest destruction was predation (8.6 percent and 15.7 percent respectively) followed by nest erosion (2.2 percent and 6.3 percent respectively). Overall nest success was high but higher for loggerheads (89 percent) than for leatherbacks (78 percent). The main cause of egg mortality for both species was early developmental arrest, followed by predation by ants and ghost crabs. Hatchlings en route to the sea were almost exclusively predated by ghost crabs (4.2 percent of emerged loggerhead hatchlings and 3.2 percent of emerged leatherback hatchlings). It appears that both species benefit from the coastal conservation efforts. When sea turtles leave the nesting beach, either as hatchlings or adults, conservation and monitoring becomes more difficult and sea turtles are exposed to a multitude of threats, including anthropogenic threats. Age classes tend to be spatially separated due to different habitat and dietary requirements. The type of threat sea turtles are exposed to thus depends on the current age class. Offshore sources of mortality in the SWIO were identified and where possible loggerhead and leatherback mortality was quantified and mapped spatially. Loggerheads were mostly exposed to and had the highest mortality in the artisanal fisheries in the SWIO (> 1000 per annum), inshore trawling (ca. 41 per annum), shark nets (protective gill nets) (21.6 ± 6.7 per annum) and the pelagic longline fishery (5.0 ± 4.4 per annum). In contrast, leatherbacks with a pelagic lifestyle, were mostly exposed to pelagic longline fisheries (7.8 ± 7.8 per annum). A spatial analysis of fishing activities indicated that leatherback home ranges overlapped 41percent with pelagic longline fishing activity in the SA EEZ, whereas the overlap between pelagic longliners and loggerhead home ranges was 29 percent. The quantified sources of mortality provide some explanation for the trend in the loggerhead nesting population but not the trend in the leatherback nesting population. Hatchling survivorship to adulthood was estimated to determine the viability of the two nesting populations as well as to determine whether offshore mortality was responsible for the difference in recovery of the two populations. Loggerhead hatchling survivorship to adulthood was estimated at between 2 and 10 per 1000 hatchlings, the minimum requirement for an increasing population. The adopted sophisticated model shows that leatherbacks have a survival rate of 5 to 10 per 1 000 hatchlings. However, this suggests that the population is increasing, but the leatherback population is stable. Perhaps the age to maturity of SA leatherbacks is greater than 12 years, or fisheries-related mortality affects younger age classes than initially thought. It is therefore recommended that the turtle monitoring area is extended to include other potential nesting grounds. In addition, observer or monitoring programs for commercial as well as artisanal fisheries needs to be extended throughout the SWIO to quantify sea turtle mortality. Ultimately a comprehensive multi-regional approach is required for the conservation of these highly migratory species.
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- Date Issued: 2012
Feeding dynamics of suspension-feeders in the nearshore marine environment adjacent to two contrasting estuaries in the Eastern Cape, South Africa
- Authors: Vermeulen, Ilke
- Date: 2012
- Subjects: Suspension feeders -- South Africa -- Eastern Cape , Suspension feeders -- Food -- South Africa -- Eastern Cape , Coastal organisms -- South Africa -- Eastern Cape , Streamflow -- South Africa -- Eastern Cape , Tetraclitidae -- South Africa -- Eastern Cape , Mexilhao mussel -- South Africa -- Eastern Cape , Polychaeta -- South Africa -- Eastern Cape , Estuarine health -- South Africa -- Eastern Cape , Estuaries -- Freshwater inflow -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5916 , http://hdl.handle.net/10962/d1016242
- Description: Coastal transition zones form important interlinking regions where marine ecosystems, rivers and estuarine environments significantly influence each other. Coastal rocky shores are key habitats that sustain a variety of primary producers and invertebrates and due to the dynamic nature of coastal ecosystems, suspension-feeders on rocky shores can be influenced by an array of autochthonous and allochthonous food sources. Fatty acid and stable isotope trophic markers were employed to distinguish between regional and temporal changes in the potential food sources to rocky shore suspension-feeders in the Eastern Cape Province of South Africa. The primary aim was to assess the spatial and temporal influences of contrasting river flows on the available food sources to three indigenous coastal suspensionfeeders, namely the volcano barnacle Tetraclita serrata, brown mussel Perna perna and tubebuilding polychaete Gunnarea capensis. This was done by examining the intra- and interspecific changes in the fatty acid and stable isotope signatures of the barnacles, mussels and polychaetes in the adjacent marine environment of a freshwater-restricted (Kariega) and freshwater-dominated (Great Fish) estuary during austral summer and winter. Multivariate and Kruskal-Wallis analyses of variance on the fatty acid and isotopic signatures, respectively, identified significant regional changes in the barnacles and mussels, while only stable isotopes distinguished between the Kariega and Great Fish polychaetes (P < 0.05). In addition, significant temporal changes were observed in consumer fatty acids and isotope values in both regions (P < 0.05). Bacterial sources, detritus and phytoplankton assemblages, which are influenced by hydrology and vegetation, differed between regions and were mainly responsible for the regional and temporal separations. Principal component analyses on the consumer fatty acid signatures distinguished between animals situated upstream (i.e. north) and downstream (i.e. south) of the Kariega Estuary mouth in summer. The north/south separation was mainly due to greater contributions of diatoms to northernlocated animals and dinoflagellates and detritus to southern-located consumers. In addition, the south-flowing Agulhas Current on the eastern shores of southern Africa appeared to influence the north/south separation in the Kariega region, as water leaving the estuary was probably entrained into the south-easterly flowing currents, thereby depositing estuarinederived detritus to southern populations. In general, diatoms and detritus were essential food sources to the filter-feeders in summer, and flagellates, diatoms, zooplankton and detritus were important in winter. Coastal macroalgae was a key food source in the Kariega and Great Fish regions during both seasons. Consistently large levels of diatom markers (16:1n-7 and 20:5n-3) and dinoflagellate markers (22:6n-3) in consumer tissues in the Kariega and Great Fish regions identified that phytoplankton was their dominant food source. The barnacles, mussels and polychaetes had similar fatty acid markers and a fairly narrow δ¹³C range (-16.5 to -14.4 ‰), suggesting that they probably consumed similar food. Carbon isotope analyses, however, separated the suspension-feeders into slightly depleted (barnacles; -16.5 to -16.1 ‰), intermediate (mussels; -15.8 to -15.0 ‰) and enriched (polychaetes; -15.0 to -14.4 ‰) consumers, but did not provide conclusive evidence of their preferences for specific phytoplankton. Conversely, fatty acid analyses highlighted that barnacles and mussels had greater proportions of dinoflagellate markers (22:6n-3; 7.0-15.3 % TFA), while polychaetes had larger diatom levels (20:5n-3; 15.1-22.2 % TFA). In addition, all three species had consistently large contributions from bacterial fatty acids (15:0, i-16:0, 17:0 and i-18:0; 4.2-13.6 % TFA) in summer and winter, and large proportions of saturated fatty acids (33.3-53.1 % TFA) including those with 14 to 18 carbons, indicating that bacterial and detritus food sources played an important role in their diets. Barnacles had small levels of terrestrial markers (18:2n-6 and 18:3n-3; <2.5 % TFA) and demonstrated increased omnivorous feeding compared with the other suspension-feeders [increased levels of 20:1n-11 and 20:1n-9, higher 18:1n-9/18:1n-7 ratios at ~2.1, enriched δ¹⁵N values at ~10.6 ‰; zooplankton (potentially including microzooplankton, larvae and protists) contribution of up to 61 % of the diet]. Mussels contained significant proportions of the terrestrial markers (18:2n-6 and 18:3n-3; >2.5 % TFA) and exhibited intermediate omnivory (intermediate levels of 20:1n-11 and 20:1n-9, intermediate 18:1n-9/18:1n-7 ratios at ~1.3, less enriched δ¹⁵N values at ~7.9 ‰; zooplankton contribution of 10-15 % of the diet). The more depleted nitrogen signatures in the mussels relative to the barnacles and polychaetes possibly illustrated a stronger preference for autotrophic food. Polychaetes mainly consumed plant food sources (i.e. microalgae, macroalgae and detritus; high levels of i-18:0, 18:1n-9, 18:4n-3 and 20:5n-3) and displayed little omnivory (low levels of 20:1n-11 and 20:1n-9, low 18:1n-9/18:1n-7 ratios at ~0.4, intermediate δ¹⁵N values at ~9.1 ‰; zooplankton contribution of <10 % of the diet). The barnacles, mussels and polychaetes are all suspension-feeders, originally presumed to consume the same food sources. The variations observed among the species, therefore, may result from differences in the proportional contributions of the various food sources to their diets as well as distinctions in metabolism. The distinct changes in the fatty acid and stable isotope signatures in all three filter-feeders in the Kariega and Great Fish regions are likely influenced by the diversity in regional vegetation and hydrology in the different systems, combined with interspecific differences in resource partitioning among the species.
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- Date Issued: 2012
Feeding ecology and dietary patterns of the Indo-Pacific bottlenose dolphin (Tursiops Aduncus) off KwaZulu-Natal, South Africa
- Authors: Kaiser, Sabina Marie Luise
- Date: 2012
- Subjects: Bottlenose dolphin -- Ecology -- South Africa -- KwaZulu-Natal , Bottlenose dolphin -- Feeding and feeds -- South Africa -- KwaZulu-Natal
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10710 , http://hdl.handle.net/10948/d1011509 , Bottlenose dolphin -- Ecology -- South Africa -- KwaZulu-Natal , Bottlenose dolphin -- Feeding and feeds -- South Africa -- KwaZulu-Natal
- Description: The Indo-Pacific bottlenose dolphin (Tursiops aduncus) is a common top predator along the KwaZulu-Natal coast. There have been several dietary studies done on this species, most of which have classed this predator as an opportunistic feeder preying on the most abundant prey species in that area. In KwaZulu-Natal (KZN) a dietary study was carried out in 1990, but there have been no followup studies to examine potential changes in diet. A number of bottlenose dolphins get entangled in the shark-nets set off the KZN coast each year. These dolphins provide a valuable source of information about different aspects of the ecology of this population. The aim of this study was to examine the diet of bottlenose dolphins off the KwaZulu-Natal coast with particular emphasis on temporal change in diet composition. The contents of 102 stomachs of bottlenose dolphins (58 females, 44 males) accidentally caught in the shark nets between 1985 and 2010 were analysed, and compared to historical data from dolphins caught between 1975 and 1986. A total of 94 different prey species from 37 families (32 fish families, five cephalopod families) were identified in the stomach contents. Teleosts made up the most important prey group in numbers and weight constituting 84 percent and 67 percent, respectively, while cephalopods made up 15 percent of the number and 30 percent of the weight of the prey items. Multi Dimensional Scaling (MDS) indicated that there is no resource partitioning between males and females, but significant partitioning between juveniles and adults. Six prey species contributed more than 60 percent of the weight of all prey species in the diet. These species are the most important according to their calculated index of relative importance score and are, in order of importance: Sepia sp. (cuttlefish)., Pomadasys olivaceus (piggy), Trachurus delagoa (maasbanker), Scomber japonicus (mackeral), Loligo sp. (squid). and Pagellus bellottii natalensis (pandora or red tjor-tjor). When compared to historic data, no changes in the main prey species has occurred, but the piggy has decreased in importance (1976-1986 percent IRI=45; 2000-2010 percent IRI=22), while the maasbanker has increased (1975-1986, percent RI=4; 2000-2010 percent RI=20). Stable carbon and nitrogen isotope analysis performed on tissue from the teeth of T. aduncus caught in the shark nets between 1970 and 2010 showed that there has been no significant shift in trophic level (δ15N) or in primary prey source (δ13C) over this time. The isotopic signatures of different tissues from an individual were used to create a base line and describe the differences in tissue signatures. Blubber was found to be significantly different from skin and muscle, which were found to be isotopically similar. Skin from remote biopsies can therefore probably be used in place of muscle for isotopic studies of bottlenose dolphins, while caution should be taken when using blubber. Given that T. aduncus is still listed as “data deficient” by the International Union for the Conservation of Nature (IUCN), and the potential for climate change and expanding fisheries to alter the distribution and abundance of their prey species, long term dietary studies such as this are important in order for their effective conservation and management.
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- Date Issued: 2012
Finite element analysis of the heat transfer in friction stir welding with experimental validation
- Authors: Vosloo, Natalie
- Date: 2012
- Subjects: Finite element method , Heat -- Transmission , Friction welding
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10511 , http://hdl.handle.net/10948/d1019981
- Description: Friction stir welding is a relatively new joining process. The heat transfer involved is crucial in determining the quality of the weld. Experimenrtal data, though important, does not provide enough information about the heat transfer process and experiments can be costly and time consuming. A numerical model, using the finite element method, was developed to stimulate the heat transfer in the workpiece in which the heat generation due to friction and plastic deformation was modelled as a surface heat flux boundary condition. This model was applied to Aluminium AL6082-T6 and Titanium Ti6A1-4V for different welding condiitions. Results were validated with experimental results. The model was shown to give better predictions of the maximum temperatures at locations in the workpiece than the overall temperature trend. A parametric study was also performed on the Aluminium model in order o predict temperature fields of the workpiece for welding conditions that were additional to those undertaken experimentally. It was found that rotational speed had a larger effect on the change in temperature than the feed rate. From the parametric study it was also clear that lower rotational speeds (300 to 660 rpm) had a greater effect on the change in temperature than the higher rotational speeds (840 to 1200 rpm). It was concluded that the model was well suited for the estimation of temperatures involved in the FSw of Aluminium Al6082-T6 but was not as accurate when applied to the FSW of Titanium.
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- Date Issued: 2012
Formulation and stability testing of eye drop preparations containing phenylephrine hydrochloride
- Authors: Okafor, Chinedum Oluchukwu
- Date: 2012
- Subjects: Phenylpropanolamine , Viscosity , Glycerin
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10164 , http://hdl.handle.net/10948/d1020985
- Description: Phenylephrine hydrochloride is a potent adrenergic agent and β-receptor sympathomimetic drug, used in its optically active form (Pandey et al., 2003; Pandey et al., 2006). As an α1-adrenergic receptor agonist, phenylephrine hydrochloride is used ocularly as a decongestant for uveitis and as an agent to dilate the pupil (Lang, 1995). High intraocular doses have been reported to cause tachycardia, hypertension, and headache. These side effects are caused by large amounts of the drop draining into the nasal cavity. Eye drops that contain phenylephrine hydrochloride have proven to have low intra-ocular bioavailability because of a short contact time with the eyes which reduces the amount of drug reaching the site of action. Formulations of phenylephrine hydrochloride eye drops have varying shelf-lives of approximately two to four years. The aim of this study was to formulate and manufacture an eye drop product containing phenylephrine hydrochloride. Important characteristics that were targeted were increased ocular absorption by increasing the viscosity of the product and reduced degradation of phenylephrine hydrochloride. A variety of phenylephrine hydrochloride formulations were manufactured on a laboratory scale using hydroxypropyl methylcellulose (HPMC), glycerol, and sodium carboxy methylcellulose as viscosity modifying agents (VMA). The concentration of phenylephrine hydrochloride was ten percent. Ten millimeters of each formulation was made in triplicate. The quantity in each was evaluated using a previously validated high performance (pressure) liquid chromatography method. Physicochemical properties including pH and colour were also evaluated. Stability was assessed using real time and accelerated stability conditions in accordance with the International Conference on Harmonization (ICH) guidelines. Formulations containing hydroxypropyl methylcellulose (HPMC) as the viscosity modifying agents proved to be stable under all storage conditions when compared with formulations containing other viscosity modifying agents (VMA). However, sodium citrate dihydrate; sodium metabisulphite and EDTA also stabilized the formulations to a certain extent Changes in the appearance and colour of products containing glycerol under accelerated storage conditions were observed. The sodium carboxy methylcellulose (SCMC) containing formulation was found to be physically and chemically stable in two conditions, namely 30 °C/65 percent RH and 25 °C/60 percent RH. The formulations containing hydroxypropyl methylcellulose along with an antioxidant showed to be most stable as it remained aesthetically pleasing did not change colour and did not have a reduction in phenylephrine hydrochloride concentrations. This meant that phenylephrine hydrochloride did not degrade while the viscosity modifying agents remained stable. Rheological tests showed differences in the viscosities of the formulations as glycerol had increased in viscosity over time while HMPC and SCMC displayed relative similarities. The formulations were compared to a marketed eye drop containing polyvinyl alcohol as a VMA. After rheological analysis the formulation containing HPMC displayed better viscosity than the product with polyvinyl alcohol. The preservatives in the formulations were active against the microbial organisms use to challenged them.
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- Date Issued: 2012
Genetic algorithm for Artificial Neural Network training for the purpose of Automated Part Recognition
- Authors: Buys, Stefan
- Date: 2012
- Subjects: Genetic algorithms , Software architecture
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:9648 , http://hdl.handle.net/10948/d1008356 , Genetic algorithms , Software architecture
- Description: Object or part recognition is of major interest in industrial environments. Current methods implement expensive camera based solutions. There is a need for a cost effective alternative to be developed. One of the proposed methods is to overcome the hardware, camera, problem by implementing a software solution. Artificial Neural Networks (ANN) are to be used as the underlying intelligent software as they have high tolerance for noise and have the ability to generalize. A colleague has implemented a basic ANN based system comprising of an ANN and three cost effective laser distance sensors. However, the system is only able to identify 3 different parts and needed hard coding changes made by trial and error. This is not practical for industrial use in a production environment where there are a large quantity of different parts to be identified that change relatively regularly. The ability to easily train more parts is required. Difficulties associated with traditional mathematically guided training methods are discussed, which leads to the development of a Genetic Algorithm (GA) based evolutionary training method that overcomes these difficulties and makes accurate part recognition possible. An ANN hybridised with GA training is introduced and a general solution encoding scheme which is used to encode the required ANN connection weights. Experimental tests were performed in order to determine the ideal GA performance and control parameters as studies have indicated that different GA control parameters can lead to large differences in training accuracy. After performing these tests, the training accuracy was analyzed by investigation into GA performance as well as hardware based part recognition performance. This analysis identified the ideal GA control parameters when training an ANN for the purpose of part recognition and showed that the ANN generally trained well and could generalize well on data not presented to it during training.
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- Date Issued: 2012
GPF : a framework for general packet classification on GPU co-processors
- Authors: Nottingham, Alastair
- Date: 2012
- Subjects: Graphics processing units , Coprocessors , Computer network protocols , Computer networks -- Security measures , NVIDIA Corporation
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4661 , http://hdl.handle.net/10962/d1006662 , Graphics processing units , Coprocessors , Computer network protocols , Computer networks -- Security measures , NVIDIA Corporation
- Description: This thesis explores the design and experimental implementation of GPF, a novel protocol-independent, multi-match packet classification framework. This framework is targeted and optimised for flexible, efficient execution on NVIDIA GPU platforms through the CUDA API, but should not be difficult to port to other platforms, such as OpenCL, in the future. GPF was conceived and developed in order to accelerate classification of large packet capture files, such as those collected by Network Telescopes. It uses a multiphase SIMD classification process which exploits both the parallelism of packet sets and the redundancy in filter programs, in order to classify packet captures against multiple filters at extremely high rates. The resultant framework - comprised of classification, compilation and buffering components - efficiently leverages GPU resources to classify arbitrary protocols, and return multiple filter results for each packet. The classification functions described were verified and evaluated by testing an experimental prototype implementation against several filter programs, of varying complexity, on devices from three GPU platform generations. In addition to the significant speedup achieved in processing results, analysis indicates that the prototype classification functions perform predictably, and scale linearly with respect to both packet count and filter complexity. Furthermore, classification throughput (packets/s) remained essentially constant regardless of the underlying packet data, and thus the effective data rate when classifying a particular filter was heavily influenced by the average size of packets in the processed capture. For example: in the trivial case of classifying all IPv4 packets ranging in size from 70 bytes to 1KB, the observed data rate achieved by the GPU classification kernels ranged from 60Gbps to 900Gbps on a GTX 275, and from 220Gbps to 3.3Tbps on a GTX 480. In the less trivial case of identifying all ARP, TCP, UDP and ICMP packets for both IPv4 and IPv6 protocols, the effective data rates ranged from 15Gbps to 220Gbps (GTX 275), and from 50Gbps to 740Gbps (GTX 480), for 70B and 1KB packets respectively. , LaTeX with hyperref package
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- Date Issued: 2012
High pressure liquid chromatographic quantification of nitrile biocatalysis
- Authors: Mathiba, Kgama
- Date: 2012
- Subjects: High performance liquid chromatography , Rhodococcus , Biocatalysis , Organic compounds -- Industrial applications
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4129 , http://hdl.handle.net/10962/d1015710
- Description: Nitrile biocatalysts are of use in the chemical and pharmaceutical industries for the synthesis of carboxyamides and carboxylic acids. In particular, the application of biocatalysts in the synthesis of single enantiomer compounds is of increasing interest, but requires novel substrate specific highly stereoselective biocatalysts. Addition to the limited toolbox of known nitrile biocatalysts requires definitive characterisation of the biocatalysts through accurate determination of the substrate profiles and quantification of activity. The accurate quantification of stereoisomers chiral mixtures to determine biocatalyst stereoselectivity remains a significant challenge due to the difficulty in separating stereoisomers by physical methods. The known nitrile metabolising organism, Rhodococcus rhodochrous ATCC BAA-870, was grown in a defined medium and harvested, providing whole cell biocatalyst. Additional biomass was disrupted to provide a cell free enzyme extract, which was put through an enzyme purification protocol to provide a solution with specific activity of 351 U.mg⁻¹. A portion of the enzyme was self immobilised using the SphereZyme™ technique. The nitrile hydratase SphereZymes™ (1.2 U.mg⁻¹ initial activity) that were prepared had pH and temperature optima of 6 and 30°C respectively, and could be recovered by repeated washing. The particles retained activity in the presence of the organic solvents isooctane and n-hexadecane saturated with 50 mM phosphate buffer (pH 7.5). An initial analytical system was devised for quantification of the nitrile hydratase activity using the non-chiral substrate benzonitrile. An improved reversed phase high performance liquid chromatography method was developed to separate and quantify benzamide, benzoic acid and benzonitrile. The mobile phase consisting of 0.1% trifluoroacetic acid in H₂O and acetonitrile (70:30, %v/v), at a flow rate of 0.5 ml.ml⁻¹, 25°C, resolved all three analytes in 3.5 minutes on a Waters X-Terra MS C18 3.5μm column. UV detection was carried out at 210 nm. Analytical methods to determine activity and enantioselectivity of the whole cell biocatalyst were subsequently developed for both β-amino nitriles and β-hydroxy nitrile substrates and hydrolysis products.
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- Date Issued: 2012
How does the ungulate community respond to predation risk from cheetah (Acinonyx jubatus) in Samara Private Game Reserve?
- Authors: Makin, Douglas Ferguson
- Date: 2012
- Subjects: Ungulates -- South Africa -- Eastern Cape , Predatory animals -- Ecology -- South Africa -- Eastern Cape , Predation (Biology) -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10722 , http://hdl.handle.net/10948/d1018461
- Description: Predator reintroductions are becoming increasingly more common for multiple reasons, including assisting with the conservation of a predator species, the restoration of ecosystem functions and the economic benefits of their reintroduction for ecotourism ventures. There remains however, little knowledge on prey species responses to these predator reintroductions. As such, the reintroduction of cheetah (Acinonyx jubatus) into Samara Private Game Reserve provided an opportunity to investigate prey responses to predator reintroduction across a range of spatial and temporal scales. More specifically, the aim of this study was to investigate the effect of cheetah predation risk on habitat use and behavioural responses of the resident ungulate community. Samara is divided into “predator present” and “predator absent” sections, providing the opportunity to conduct a comparative study investigating the effect of cheetah on prey responses. It was hypothesized that different ungulate species would respond differently to the presence of cheetah, depending on differences in perceived vulnerability to cheetah predation. To address this, shifts in habitat use, and behavioural responses of the ungulate community reflected at landscape and patch scale were investigated. Overall, ungulate species have not shifted habitat use since cheetah reintroduction, this was possibly related to life history strategy constraints and the need for individuals to obtain suitable forage and therefore remain in specific habitats. While no shift in habitat use was observed for the majority of ungulate species, a shift in behaviour was observed for kudu (Tragelaphus strepsiceros) at a landscape scale, where kudu dedicated more time to vigilance and less time to foraging within the predator section. In addition, individuals within smaller kudu groups were observed to be more vigilant than individuals within larger groups of kudu within the predator section. Although the other three ungulate species monitored did not increase time spent vigilant within the predator section, they still maintained relatively high levels of vigilance, potentially as a means of social monitoring. At a patch level, ungulate species responded strongly to a predator cue as a proxy for cheetah proximity, and increased time spent vigilant with a trade-off of lower foraging effort. This vigilant response was strongest for kudu. Differences in perceived predation risk were reflected within eland (Tragelaphus oryx) and kudu species demographic classes, with juvenile eland and kudu, adult female kudu and subadult female kudu spending more time vigilant within manipulated patches than respective males of each species. Kudu were also observed adopting fine-scale behavioural responses to minimize predation risk within patches. The asymmetrical prey species response to perceived predation risk from cheetah supports the hypothesis that different species respond differently to the presence of a predator. Furthermore, this study illustrated the importance of measuring prey responses to predation risk across multiple scales and highlighted the need to replicate this study for a number of different sites where predators have been reintroduced, to better understand the range of factors influencing these predator-prey interactions.
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- Date Issued: 2012
Identifying volatile emissions associated with False Codling Moth infested citrus fruit
- Authors: Van der Walt, Rachel
- Date: 2012
- Subjects: Citrus -- Diseases and pests , Insect pests , Cryptophlebia leucotreta
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10343 , http://hdl.handle.net/10948/d1020056
- Description: False codling moth is a known pest of economic importance to many cultivated crops in South Africa and Africa south of the Sahara, and is particularly severe on citrus. If the fruit is infested just before harvest the chances of detecting signs of infestation are very low. As a result, the risk of packaging infested fruit and exporting them as healthy fruit is high. It is therefore a priority to develop a post-harvest technique for detection of False codling moth in citrus fruit at different levels of infestation in order to reduce phytosanitary risk. Compounds released and detected were indicative of infestation and were not insect produced but naturally produced fruit volatiles emitted at higher levels as a result of the insect within the fruit. Five major volatile compounds of interest were released by the infested oranges. These major volatile compounds include D-limonene, 3,7-dimethyl-1,3,6-octatriene, (E)-4,8-dimethyl-1,3,7-nonatriene, caryophyllene and naphthalene. Limonene was one of the most abundant volatile compounds released by the infested citrus fruit. Naphthalene, which is possibly produced due to larval feeding and development within the fruit maintained higher concentrations than controls throughout the infestation within the fruit. Naphthalene would be a good indicator of False codling moth infestation, however, not primarily for early infestation detection. A significantly higher concentration of D-limonene, 3,7-dimethyl-1,3,6-octatriene, (E)-4,8-dimethyl-1,3,7-nonatriene and naphthalene was detected using the SEP over the SPME technique. The application of an SPME procedure and the utilization of this method for detection of volatiles present in the headspace of intact infested fruit are evaluated and the possible volatile compounds diagnostic of Thaumatotibia leucotreta infestation of orange fruit and differences in volatile compound response in different orange varieties is discussed.
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- Date Issued: 2012