Development of a protocol for extracting and quantifying the concentration of thiafentanil in blesbok (Damaliscus pygargus phillipsi) matrices 72-74 hours post administration
- Authors: Webber, Judith Tracy
- Date: 2020
- Subjects: Uncatalogued
- Language: English
- Type: thesis , text , Masters , MSc
- Identifier: http://hdl.handle.net/10962/164738 , vital:41159
- Description: Thesis (MSc)--Rhodes University, Faculty of Science, Chemistry, 2020
- Full Text:
- Date Issued: 2020
- Authors: Webber, Judith Tracy
- Date: 2020
- Subjects: Uncatalogued
- Language: English
- Type: thesis , text , Masters , MSc
- Identifier: http://hdl.handle.net/10962/164738 , vital:41159
- Description: Thesis (MSc)--Rhodes University, Faculty of Science, Chemistry, 2020
- Full Text:
- Date Issued: 2020
Development of graphene materials and phthalocyanines for application in dye-sensitized solar cells
- Authors: Chindeka, Francis
- Date: 2020
- Subjects: Dye-sensitized solar cells , Graphene , Phthalocyanines , Molecular orbitals , Impedance spectroscopy
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/166092 , vital:41328
- Description: Two sets of dye-sensitized solar cells (DSSCs) were fabricated. In the first set, dye-sensitized solar cells (DSSC) were fabricated by incorporating graphene materials as catalysts at the counter electrode. Platinum was also used as a catalyst for comparative purposes. Different phthalocyanines: hydroxyl indium tetracarboxyphenoxy phthalocyanine (1), chloro indium octacarboxy phthalocyanine (2) and dibenzoic acid silicon phthalocyanine (3) were used as dyes. Complex 3 gave the highest power conversion efficiency (η) of 3.19% when using nitrogen doped reduced graphene oxide nanosheets (NrGONS) as a catalyst at the counter electrode, and TiO2 containing rGONS at the anode. The value obtained is close to 3.8% obtained when using Pt catalyst instead of NrGONS at the cathode, thus confirming that NrGONS is a promising candidate to replace the more expensive Pt. The study also shows that placing rGONS on both the anode and cathode improves efficiency. In the second set, DSSCs were fabricated by using 2(3,5-biscarboxyphenoxy), 9(10), 16(17), 23(24)-tri(tertbutyl) phthalocyaninato Cu (4) and Zn (5) complexes as dyes on the ITO-TiO2 photoanodes containing reduced graphene oxide nanosheets (rGONS) or nitrogen-doped rGONS (NrGONS). The evaluation of the assembled DSSCs revealed that using ITO-TiO2-NrGONS-CuPc (4) photoanode had the highest fill factor (FF) and power conversion efficiency (ɳ) of 69 % and 4.36 % respectively. These results show that the asymmetrical phthalocyanine complexes (4) and (5) showed significant improvement on the performance of the DSSC compared to previous work on symmetrical carboxylated phthalocyanines with ɳ = 3.19%.
- Full Text:
- Date Issued: 2020
- Authors: Chindeka, Francis
- Date: 2020
- Subjects: Dye-sensitized solar cells , Graphene , Phthalocyanines , Molecular orbitals , Impedance spectroscopy
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/166092 , vital:41328
- Description: Two sets of dye-sensitized solar cells (DSSCs) were fabricated. In the first set, dye-sensitized solar cells (DSSC) were fabricated by incorporating graphene materials as catalysts at the counter electrode. Platinum was also used as a catalyst for comparative purposes. Different phthalocyanines: hydroxyl indium tetracarboxyphenoxy phthalocyanine (1), chloro indium octacarboxy phthalocyanine (2) and dibenzoic acid silicon phthalocyanine (3) were used as dyes. Complex 3 gave the highest power conversion efficiency (η) of 3.19% when using nitrogen doped reduced graphene oxide nanosheets (NrGONS) as a catalyst at the counter electrode, and TiO2 containing rGONS at the anode. The value obtained is close to 3.8% obtained when using Pt catalyst instead of NrGONS at the cathode, thus confirming that NrGONS is a promising candidate to replace the more expensive Pt. The study also shows that placing rGONS on both the anode and cathode improves efficiency. In the second set, DSSCs were fabricated by using 2(3,5-biscarboxyphenoxy), 9(10), 16(17), 23(24)-tri(tertbutyl) phthalocyaninato Cu (4) and Zn (5) complexes as dyes on the ITO-TiO2 photoanodes containing reduced graphene oxide nanosheets (rGONS) or nitrogen-doped rGONS (NrGONS). The evaluation of the assembled DSSCs revealed that using ITO-TiO2-NrGONS-CuPc (4) photoanode had the highest fill factor (FF) and power conversion efficiency (ɳ) of 69 % and 4.36 % respectively. These results show that the asymmetrical phthalocyanine complexes (4) and (5) showed significant improvement on the performance of the DSSC compared to previous work on symmetrical carboxylated phthalocyanines with ɳ = 3.19%.
- Full Text:
- Date Issued: 2020
Distribution, habitat associations and conservation of the Eastern Cape rocky Sandelia bainsii (Perciform: Anabantidae) in the Great Fish River system
- Authors: Sifundza, Delsy Sindy
- Date: 2020
- Subjects: Anabantidae -- South Africa -- Great Fish River , Perciformes -- South Africa -- Great Fish River , Labyrinth fishes -- South Africa -- Great Fish River , Fishes -- Effect of human beings on -- South Africa -- Great Fish River , Fish populations -- South Africa -- Great Fish River , Fish declines -- South Africa -- Great Fish River , Fish populations -- Monitoring -- South Africa -- Great Fish River , Anabantidae -- Evolution -- South Africa -- Great Fish River , Gene flow , Mitochondrial DNA , Microsatellites (Genetics) , Eastern Cape rocky Sandelia bainsii , Freshwater ecology -- South Africa -- Great Fish River
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/142692 , vital:38102
- Description: In South Africa, freshwater ecosystems are under pressure due to increasing anthropogenic factors degrading river systems. The Cape Fold, the lower Southern Temperate Highveld and the Amatolo – Winterberg Highlands freshwater ecoregions contain some of the heavily impacted river systems in the country. One such river system is the Great Fish River system, which has been affected by anthropogenic modification (hydrological modification, water pollution and invasion by non-native fish species). These modifications have raised conservation concerns on an imperilled anabantid fish, the Eastern Cape rocky Sandelia bainsii that is confined to the Kat and Koonap rivers, which are tributaries of the Great Fish River. Historically, the species was reportedly common and abundant, with a range spanning across seven river systems (Kowie, Great Fish, Keiskamma, Igoda, Gxulu, Buffalo and Nahoon rivers) in the Eastern Cape Province. However, surveys have indicated that the species has experienced decline in population size and distribution range, with localised extirpations being reported. This resulted in its listing on the IUCN Red List of threatened species as Endangered. The proposed shale gas exploration and potential infrastructure development in the Karoo basin, which encompasses the headwaters of the Kat River, represent a potential future threat to the remnant populations of this species. The aim of the present study was to review the past and present distribution range of S. bainsii, determine the habitat associations and assess the conservation of S. bainsii in the Great Fish River system. This information is essential for informing conservation and management decisions for this species in the Great Fish River system. This study undertook a comprehensive survey of the Kat and the Koonap rivers in 2017, and augmented this information with data from more recent surveys that were conducted from 2009 - 2014 to map the distributions freshwater fishes in the Great Fish River system. Data from these recent surveys (2009 – 2017) were compared with historical records (1961 – 2005) obtained from the South African Institute for Aquatic Biodiversity to evaluate changes in distribution patterns of S. bainsii. Historical records indicated that S. bainsii occurred at 11 localities in the Kat River and three localities in the Koonap River. Data from recent surveys indicated that the species has persisted at these historical localities, except one locality in the Koonap River where no individuals of S. bainsii were captured, despite the use of multiple sampling approaches and gears. Although the species still persists at two of the three historical localities in the Koonap River, the population sizes have considerably declined as the species was reportedly in high abundance at these localities in the 1980s. Factors threatening the continued existence of this species in the Koonap River likely include non-native species, extreme drought conditions and the associated excessive abstraction of water which has resulted in the drying of some of the refugia pools where the species was historically common and abundant. In contrast to the Koonap River, the 2017 survey indicated that in the Kat River, S. bainsii had a similar distribution range compared to historical records. The species occurred at 11 of the 41 localities that were sampled in the Kat River, and the sampled populations consisted of all size classes. Sandelia bainsii occurred with other native fish species in the system, including Labeo umbratus, Enteromius anoplus and Glossogobius callidus. A comparison of historical and recent data indicates that non-native fishes have likely not expanded their ranges in the Kat River. This is likely due to the presence of weirs and dams that have prevented upstream movement of non-native fish species L. aeneus, C. gariepinus and T. sparrmanii which are now abundant in the lower Kat and mainstem Great Fish River. Surveys from the present study indicated that although S. bainsii still persists within the Kat River, there are a number of factors that pose a considerable threat to the continued existence of this species in the Great Fish River system. Chapter 3 evaluated the habitat associations of S. bainsii based on 10 physical and chemical variables and distribution data collected from 30 localities in the Upper Kat River and seven of its tributaries. A non-metric multidimensional scaling model was used to assess the distribution patterns of S. bainsii in relation to environmental variables. Furthermore, a negative binomial generalised linear model (GLM) was used to assess the relationship between the abundance of S. bainsii and environmental variables. Results from the present study indicated that S. bainsii was a habitat generalist as the distribution of this species showed no specific association with any of the habitat variables that were considered in this study. The species occurred in a wide range of habitats, including rocky sections in the upper Kat River and its tributaries as well as the mainstem sections. The GLM however indicated that the abundance of S. bainsii was negatively associated with increasing conductivity. In addition, the Kat River contains multiple weirs of variable sizes. Future studies should therefore evaluate the effects of these weirs on population fragmentation, gene flow and long-term evolutionary potential of S. bainsii through the application of techniques such as mitochondrial DNA and microsatellites.
- Full Text:
- Date Issued: 2020
- Authors: Sifundza, Delsy Sindy
- Date: 2020
- Subjects: Anabantidae -- South Africa -- Great Fish River , Perciformes -- South Africa -- Great Fish River , Labyrinth fishes -- South Africa -- Great Fish River , Fishes -- Effect of human beings on -- South Africa -- Great Fish River , Fish populations -- South Africa -- Great Fish River , Fish declines -- South Africa -- Great Fish River , Fish populations -- Monitoring -- South Africa -- Great Fish River , Anabantidae -- Evolution -- South Africa -- Great Fish River , Gene flow , Mitochondrial DNA , Microsatellites (Genetics) , Eastern Cape rocky Sandelia bainsii , Freshwater ecology -- South Africa -- Great Fish River
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/142692 , vital:38102
- Description: In South Africa, freshwater ecosystems are under pressure due to increasing anthropogenic factors degrading river systems. The Cape Fold, the lower Southern Temperate Highveld and the Amatolo – Winterberg Highlands freshwater ecoregions contain some of the heavily impacted river systems in the country. One such river system is the Great Fish River system, which has been affected by anthropogenic modification (hydrological modification, water pollution and invasion by non-native fish species). These modifications have raised conservation concerns on an imperilled anabantid fish, the Eastern Cape rocky Sandelia bainsii that is confined to the Kat and Koonap rivers, which are tributaries of the Great Fish River. Historically, the species was reportedly common and abundant, with a range spanning across seven river systems (Kowie, Great Fish, Keiskamma, Igoda, Gxulu, Buffalo and Nahoon rivers) in the Eastern Cape Province. However, surveys have indicated that the species has experienced decline in population size and distribution range, with localised extirpations being reported. This resulted in its listing on the IUCN Red List of threatened species as Endangered. The proposed shale gas exploration and potential infrastructure development in the Karoo basin, which encompasses the headwaters of the Kat River, represent a potential future threat to the remnant populations of this species. The aim of the present study was to review the past and present distribution range of S. bainsii, determine the habitat associations and assess the conservation of S. bainsii in the Great Fish River system. This information is essential for informing conservation and management decisions for this species in the Great Fish River system. This study undertook a comprehensive survey of the Kat and the Koonap rivers in 2017, and augmented this information with data from more recent surveys that were conducted from 2009 - 2014 to map the distributions freshwater fishes in the Great Fish River system. Data from these recent surveys (2009 – 2017) were compared with historical records (1961 – 2005) obtained from the South African Institute for Aquatic Biodiversity to evaluate changes in distribution patterns of S. bainsii. Historical records indicated that S. bainsii occurred at 11 localities in the Kat River and three localities in the Koonap River. Data from recent surveys indicated that the species has persisted at these historical localities, except one locality in the Koonap River where no individuals of S. bainsii were captured, despite the use of multiple sampling approaches and gears. Although the species still persists at two of the three historical localities in the Koonap River, the population sizes have considerably declined as the species was reportedly in high abundance at these localities in the 1980s. Factors threatening the continued existence of this species in the Koonap River likely include non-native species, extreme drought conditions and the associated excessive abstraction of water which has resulted in the drying of some of the refugia pools where the species was historically common and abundant. In contrast to the Koonap River, the 2017 survey indicated that in the Kat River, S. bainsii had a similar distribution range compared to historical records. The species occurred at 11 of the 41 localities that were sampled in the Kat River, and the sampled populations consisted of all size classes. Sandelia bainsii occurred with other native fish species in the system, including Labeo umbratus, Enteromius anoplus and Glossogobius callidus. A comparison of historical and recent data indicates that non-native fishes have likely not expanded their ranges in the Kat River. This is likely due to the presence of weirs and dams that have prevented upstream movement of non-native fish species L. aeneus, C. gariepinus and T. sparrmanii which are now abundant in the lower Kat and mainstem Great Fish River. Surveys from the present study indicated that although S. bainsii still persists within the Kat River, there are a number of factors that pose a considerable threat to the continued existence of this species in the Great Fish River system. Chapter 3 evaluated the habitat associations of S. bainsii based on 10 physical and chemical variables and distribution data collected from 30 localities in the Upper Kat River and seven of its tributaries. A non-metric multidimensional scaling model was used to assess the distribution patterns of S. bainsii in relation to environmental variables. Furthermore, a negative binomial generalised linear model (GLM) was used to assess the relationship between the abundance of S. bainsii and environmental variables. Results from the present study indicated that S. bainsii was a habitat generalist as the distribution of this species showed no specific association with any of the habitat variables that were considered in this study. The species occurred in a wide range of habitats, including rocky sections in the upper Kat River and its tributaries as well as the mainstem sections. The GLM however indicated that the abundance of S. bainsii was negatively associated with increasing conductivity. In addition, the Kat River contains multiple weirs of variable sizes. Future studies should therefore evaluate the effects of these weirs on population fragmentation, gene flow and long-term evolutionary potential of S. bainsii through the application of techniques such as mitochondrial DNA and microsatellites.
- Full Text:
- Date Issued: 2020
Do submarine canyons influence the structure and diversity of benthic fish assemblages on the continental shelf edge?
- Nyawo, Mpilonhle Aura June-Rose
- Authors: Nyawo, Mpilonhle Aura June-Rose
- Date: 2020
- Subjects: Submarine valleys , iSimangaliso Wetland Park (South Africa) , Biotic communities -- South Africa -- iSimangaliso Wetland Park , Groundfishes -- South Africa -- iSimangaliso Wetland Park , Marine ecology -- South Africa -- iSimangaliso Wetland Park
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/167264 , vital:41462
- Description: Marine ecosystems play an important role in human welfare, such as providing food security and providing social, economic and environmental benefits to an increasing human population. However, due to the deleterious impact of overfishing as well as habitat degradation, pollution and climate change, many marine ecosystems and the substantial biodiversity they support, are under increasing threats. Submarine canyon ecosystems cut into the continental margin in all our oceans and are considered regions of enhanced diversity, abundance and biomass for many marine communities, including fish. In South Africa, the tropical Delagoa Ecoregion is characterised by numerous submarine canyons that are home to coelacanths Latimeria chalumnae, and a variety of commercially important line fish species. However, there is currently a lack of information on the ecological structure and functioning of these canyons, in relation to nearby non-canyon ecosystems. The aim of this thesis was to generate knowledge on the role of submarine canyons in influencing the benthic fish assemblage structure and diversity on the continental shelf and shelf edge within the high-latitude coral reef ecosystems in the tropical Delagoa Ecoregion of South Africa. First, a systematic literature review was conducted to examine the current knowledge on the role of submarine canyons on the benthic fish assemblages occurring on continental margins, as well as outline the trends in canyon research. For the fieldwork component of this thesis, baited remote underwater stereo-video systems were utilised to gather data. The first research chapter, chapter 3, investigated the effect of ecosystem and depth on the diversity and structure of benthic fish assemblages within iSimangaliso Wetland Park in the Delagoa Ecoregion. Data were collected in 2017 from Wright Canyon (canyon ecosystem) and adjacent slope ecosystems around the canyon (non-canyon ecosystem) at depths ranging between 70–240 m. The second research chapter, chapter 4, data were collected in 2018, from 60–100 m depths in three ubmarine canyons (Wright, Diepgat,Levens) and their adjacent habitat within iSimangaliso Wetland Park to investigate the diversity patterns and community structure of benthic fish and cons ider location as a factor. The systematic review showed that canyon research is still very much in its infancy, however more papers are expected to be published in the future. There were clear location associated biases detected, with more than 90% of the canyon research being concentrated in the Mediterranean Sea, while only one study was published from South Africa. Most of the reviewed literature focused on invertebrates, with benthic fish (including cartilaginous fish) being the second most studied taxa. A wide range of methods from both fishery- dependent and fishery-independent categories were used to gather the data of benthic fish from the canyons and adjacent areas. With over 8500 submarine canyons mapped globally, the number of submarine canyon research published and the detected location bias is of great concern. More studies are needed in order to understand the role of submarine canyons on adjacent slope habitats Overall, the results from the research chapters indicated that there were higher a bundances in the canyon ecosystem compared to the non-canyon ecosystem. In contrast, evenness and beta-diversity were higher in the non-canyon ecosystem. Relief, bottom type as well as depth played a major role in explaining the observed abundance and diversity patterns. Both evenness and beta-diversity decreased with increase in depth. The canyon and non-canyon also differed in terms of habitat structure. The canyon ecosystem was characterised by patched reef and reef habitats with high relief whereas the non-canyon ecosystem was characterised by flats and habitats, especially at shallower depths. Additionally, there were clear differences in terms of the fish community structure between the canyon and the non-canyon ecosystem. The heterogeneous and complex habitats of the canyon ecosystem were typified by species such as Epinephelus poecilonotus, Polysteganus coeruleopunctatus and Chrysoblephus puniceus in comparison to the less complex habitats outside the canyons that were preferred by species such as Lagocephalus sceleratus, and Rhinobatos leucospilus. The different locations sampled demonstrated that the canyon effect was not onsistent at all locations, with varying results detected in the different sample locations. This research demonstrates that clea r differences exists between the benthic fish assemblages associated with canyon and non-canyon ecosystems and provides important information on the role of submarine canyons in the iSimangaliso Wetland Park. From this thesis, it is clear that a ‘canyon effect’ exists in the continental shelf and shelf edge. The results suggested that heterogeneity of habitats was the most important environmental factor that differed between the canyon and non-canyon ecosystems and these differences in the habitat were driving the observed patterns. This thesis has provided a reference point, with regards to investigating the role and influence of these canyons on benthic fish assemblages occurring on the continental shelf edge. The information provided by this thesis can ultimately be used in other studies investigating benthic fish assemblages in other submarine canyons within iSimangaliso Wetland Park and the rest of South Africa./
- Full Text:
- Date Issued: 2020
- Authors: Nyawo, Mpilonhle Aura June-Rose
- Date: 2020
- Subjects: Submarine valleys , iSimangaliso Wetland Park (South Africa) , Biotic communities -- South Africa -- iSimangaliso Wetland Park , Groundfishes -- South Africa -- iSimangaliso Wetland Park , Marine ecology -- South Africa -- iSimangaliso Wetland Park
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/167264 , vital:41462
- Description: Marine ecosystems play an important role in human welfare, such as providing food security and providing social, economic and environmental benefits to an increasing human population. However, due to the deleterious impact of overfishing as well as habitat degradation, pollution and climate change, many marine ecosystems and the substantial biodiversity they support, are under increasing threats. Submarine canyon ecosystems cut into the continental margin in all our oceans and are considered regions of enhanced diversity, abundance and biomass for many marine communities, including fish. In South Africa, the tropical Delagoa Ecoregion is characterised by numerous submarine canyons that are home to coelacanths Latimeria chalumnae, and a variety of commercially important line fish species. However, there is currently a lack of information on the ecological structure and functioning of these canyons, in relation to nearby non-canyon ecosystems. The aim of this thesis was to generate knowledge on the role of submarine canyons in influencing the benthic fish assemblage structure and diversity on the continental shelf and shelf edge within the high-latitude coral reef ecosystems in the tropical Delagoa Ecoregion of South Africa. First, a systematic literature review was conducted to examine the current knowledge on the role of submarine canyons on the benthic fish assemblages occurring on continental margins, as well as outline the trends in canyon research. For the fieldwork component of this thesis, baited remote underwater stereo-video systems were utilised to gather data. The first research chapter, chapter 3, investigated the effect of ecosystem and depth on the diversity and structure of benthic fish assemblages within iSimangaliso Wetland Park in the Delagoa Ecoregion. Data were collected in 2017 from Wright Canyon (canyon ecosystem) and adjacent slope ecosystems around the canyon (non-canyon ecosystem) at depths ranging between 70–240 m. The second research chapter, chapter 4, data were collected in 2018, from 60–100 m depths in three ubmarine canyons (Wright, Diepgat,Levens) and their adjacent habitat within iSimangaliso Wetland Park to investigate the diversity patterns and community structure of benthic fish and cons ider location as a factor. The systematic review showed that canyon research is still very much in its infancy, however more papers are expected to be published in the future. There were clear location associated biases detected, with more than 90% of the canyon research being concentrated in the Mediterranean Sea, while only one study was published from South Africa. Most of the reviewed literature focused on invertebrates, with benthic fish (including cartilaginous fish) being the second most studied taxa. A wide range of methods from both fishery- dependent and fishery-independent categories were used to gather the data of benthic fish from the canyons and adjacent areas. With over 8500 submarine canyons mapped globally, the number of submarine canyon research published and the detected location bias is of great concern. More studies are needed in order to understand the role of submarine canyons on adjacent slope habitats Overall, the results from the research chapters indicated that there were higher a bundances in the canyon ecosystem compared to the non-canyon ecosystem. In contrast, evenness and beta-diversity were higher in the non-canyon ecosystem. Relief, bottom type as well as depth played a major role in explaining the observed abundance and diversity patterns. Both evenness and beta-diversity decreased with increase in depth. The canyon and non-canyon also differed in terms of habitat structure. The canyon ecosystem was characterised by patched reef and reef habitats with high relief whereas the non-canyon ecosystem was characterised by flats and habitats, especially at shallower depths. Additionally, there were clear differences in terms of the fish community structure between the canyon and the non-canyon ecosystem. The heterogeneous and complex habitats of the canyon ecosystem were typified by species such as Epinephelus poecilonotus, Polysteganus coeruleopunctatus and Chrysoblephus puniceus in comparison to the less complex habitats outside the canyons that were preferred by species such as Lagocephalus sceleratus, and Rhinobatos leucospilus. The different locations sampled demonstrated that the canyon effect was not onsistent at all locations, with varying results detected in the different sample locations. This research demonstrates that clea r differences exists between the benthic fish assemblages associated with canyon and non-canyon ecosystems and provides important information on the role of submarine canyons in the iSimangaliso Wetland Park. From this thesis, it is clear that a ‘canyon effect’ exists in the continental shelf and shelf edge. The results suggested that heterogeneity of habitats was the most important environmental factor that differed between the canyon and non-canyon ecosystems and these differences in the habitat were driving the observed patterns. This thesis has provided a reference point, with regards to investigating the role and influence of these canyons on benthic fish assemblages occurring on the continental shelf edge. The information provided by this thesis can ultimately be used in other studies investigating benthic fish assemblages in other submarine canyons within iSimangaliso Wetland Park and the rest of South Africa./
- Full Text:
- Date Issued: 2020
Early diagnosis of colorectal cancer using gold nanoparticles : a pilot study
- Authors: Noguda, Njabulo
- Date: 2020
- Subjects: Colon (Anatomy) -- Cancer -- Diagnosis
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10948/48577 , vital:40892
- Description: Introduction: Colorectal cancer (CRC) is ranked amongst the top three most diagnosed cancers in humans worldwide. Colorectal cancer is the fourth most common cancer in South Africa and the sixth most common cause of death. If diagnosed during the early stages, the 5year relative survival rate can be as high as 90%. However, in most cases, the diagnosis is only made at a later stage when the cancer has metastasised, as the detection depends on the symptoms and non-specific screening. Many attempts have been made to achieve an early detected of CRC. Aim: To induce CRC in rats to investigate the use of targeted gold nanoparticles (AuNPs) as a diagnostic tool for CRC. Methods: Ten Wistar rats were injected with 1,2-dimethylhydrazine (DMH), 25 µg/Kg bodyweight once per week, for 5 consecutive weeks to induce CRC. This was followed by an incubation period of 22 weeks. At the end of the incubation period, AuNPs were synthesized using Turkevich’s citrate reduction method, and conjugated with peptide p.L. The rats were then injected intraperitoneally with p.L-PEG-AuNPs to detect CRC. Six rats were kept as controls and did not receive any treatment. Results: Five injections of DMH did not induce CRC in the rats. Conclusion: Even though CRC could not be induced using DMH in this study, 14 nm p.L-AuNPs were synthesized successfully.
- Full Text:
- Date Issued: 2020
- Authors: Noguda, Njabulo
- Date: 2020
- Subjects: Colon (Anatomy) -- Cancer -- Diagnosis
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10948/48577 , vital:40892
- Description: Introduction: Colorectal cancer (CRC) is ranked amongst the top three most diagnosed cancers in humans worldwide. Colorectal cancer is the fourth most common cancer in South Africa and the sixth most common cause of death. If diagnosed during the early stages, the 5year relative survival rate can be as high as 90%. However, in most cases, the diagnosis is only made at a later stage when the cancer has metastasised, as the detection depends on the symptoms and non-specific screening. Many attempts have been made to achieve an early detected of CRC. Aim: To induce CRC in rats to investigate the use of targeted gold nanoparticles (AuNPs) as a diagnostic tool for CRC. Methods: Ten Wistar rats were injected with 1,2-dimethylhydrazine (DMH), 25 µg/Kg bodyweight once per week, for 5 consecutive weeks to induce CRC. This was followed by an incubation period of 22 weeks. At the end of the incubation period, AuNPs were synthesized using Turkevich’s citrate reduction method, and conjugated with peptide p.L. The rats were then injected intraperitoneally with p.L-PEG-AuNPs to detect CRC. Six rats were kept as controls and did not receive any treatment. Results: Five injections of DMH did not induce CRC in the rats. Conclusion: Even though CRC could not be induced using DMH in this study, 14 nm p.L-AuNPs were synthesized successfully.
- Full Text:
- Date Issued: 2020
Ecological engineering: an assessment of the ecological impact of Reno mattress structures used in erosion control in the Keurbooms Estuary, South Africa
- Authors: De Villiers, Nina
- Date: 2020
- Subjects: Sediments (Geology) -- Management , Sediments (Geology) -- South Africa -- Keurboomstrand , Coast changes -- South Africa -- Keurboomstrand , Shore protection -- South Africa -- Keurboomstrand , Coastal engineering-- South Africa -- Keurboomstrand , Coastal zone management-- South Africa -- Keurboomstrand , Estuarine ecology-- South Africa -- Keurboomstrand , Eelgrass -- South Africa -- Keurboomstrand , Reno Mattresses
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/166138 , vital:41332
- Description: Global climate changes have been associated with ocean warming and sea-level rise. Armouring of coastlines has become common practice with the increasing threat of coastal erosion. The transformation of soft sediment habitats to hard, artificial habitats because of coastline armouring can lead to changes in species diversity, composition and distribution. It is, therefore, essential to assess changes to habitats from coastal development as well as the ecological impact erosion control structures have within coastal systems. Ecological engineering attempts to combine engineering principals and ecological processes to reduce environmental impacts from coastal development and the implementation of artificial structures. Estuaries are particularly vulnerable to anthropogenic impacts through development, and are extremely important systems offering nursery and foraging grounds for many species. These systems are, however, particularly vulnerable to anthropogenic impacts from urbanisation and development. Within South Africa many estuaries are being transformed by the addition of artificial structures to combat erosion, one such structure is the Reno mattress (a flattened wire box filled with rocks). This study compared the fish diversity and abundances of existing Reno mattress structures and natural eelgrass (Zostera capensis) habitat in the Keurbooms Estuary, South Africa. Benthic invertebrates were sampled using standard core sampling and an adapted suction sampling approach within the two habitats. The non-destructive method of mini Baited Remote Underwater Video Systems (BRUVs) was used to sample fish. Seasonal benthic invertebrate and fish abundances and assemblages were assessed from winter 2018 to spring 2019 with greater abundances of both recorded in summer. Significantly greater abundances, diversity, and richness of fish were found in the Reno mattress habitat compared to Z. capensis. Invertebrate taxa displayed some overlap between habitats, however, three higher taxonomic groups were only recorded within Reno mattress habitat and one only within the eelgrass habitat. Fish assemblages differed significantly between the two established habitats. A Before-After-Control-Impact (BACI) investigation was used to assess the ecological impact of newly installed Reno mattresses in the Keurbooms Estuary. Zostera capensis extent was sampled by determining the percentage cover of 0.5 m X 0.5 m quadrats and measurements of eelgrass blades. Percentage cover and blade length decreased during the installation of Reno mattress, but then recovered shortly after completion of the installation. Abundances, richness and diversity of invertebrates and fish were found to be similar before and after the installation which suggests that the installation had no net negative impact on the site. The Reno mattresses were found to attract fauna typical of rocky shore environments as well as a few invasive alien invertebrate species. This study noted that a hybrid habitat of Reno mattress and eelgrass was created and may in fact provide the positives of both to a system. In any coastal development it will be important to balance the demands of a growing population and the protection of natural habitats. The results of this study suggest that complex artificial structures such as Reno mattresses do provide habitat for fish and invertebrates. However, the use of these structures should be in combination with natural vegetation (e.g. as a hybrid habitat) and not one that replaces intertidal and subtidal natural habitat especially eelgrass. There is limited information regarding the ecological impacts of using Reno mattresses in estuaries and this study provides new information on their ecological efficacy that should be valuable for future coastal erosion control practices.
- Full Text:
- Date Issued: 2020
- Authors: De Villiers, Nina
- Date: 2020
- Subjects: Sediments (Geology) -- Management , Sediments (Geology) -- South Africa -- Keurboomstrand , Coast changes -- South Africa -- Keurboomstrand , Shore protection -- South Africa -- Keurboomstrand , Coastal engineering-- South Africa -- Keurboomstrand , Coastal zone management-- South Africa -- Keurboomstrand , Estuarine ecology-- South Africa -- Keurboomstrand , Eelgrass -- South Africa -- Keurboomstrand , Reno Mattresses
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/166138 , vital:41332
- Description: Global climate changes have been associated with ocean warming and sea-level rise. Armouring of coastlines has become common practice with the increasing threat of coastal erosion. The transformation of soft sediment habitats to hard, artificial habitats because of coastline armouring can lead to changes in species diversity, composition and distribution. It is, therefore, essential to assess changes to habitats from coastal development as well as the ecological impact erosion control structures have within coastal systems. Ecological engineering attempts to combine engineering principals and ecological processes to reduce environmental impacts from coastal development and the implementation of artificial structures. Estuaries are particularly vulnerable to anthropogenic impacts through development, and are extremely important systems offering nursery and foraging grounds for many species. These systems are, however, particularly vulnerable to anthropogenic impacts from urbanisation and development. Within South Africa many estuaries are being transformed by the addition of artificial structures to combat erosion, one such structure is the Reno mattress (a flattened wire box filled with rocks). This study compared the fish diversity and abundances of existing Reno mattress structures and natural eelgrass (Zostera capensis) habitat in the Keurbooms Estuary, South Africa. Benthic invertebrates were sampled using standard core sampling and an adapted suction sampling approach within the two habitats. The non-destructive method of mini Baited Remote Underwater Video Systems (BRUVs) was used to sample fish. Seasonal benthic invertebrate and fish abundances and assemblages were assessed from winter 2018 to spring 2019 with greater abundances of both recorded in summer. Significantly greater abundances, diversity, and richness of fish were found in the Reno mattress habitat compared to Z. capensis. Invertebrate taxa displayed some overlap between habitats, however, three higher taxonomic groups were only recorded within Reno mattress habitat and one only within the eelgrass habitat. Fish assemblages differed significantly between the two established habitats. A Before-After-Control-Impact (BACI) investigation was used to assess the ecological impact of newly installed Reno mattresses in the Keurbooms Estuary. Zostera capensis extent was sampled by determining the percentage cover of 0.5 m X 0.5 m quadrats and measurements of eelgrass blades. Percentage cover and blade length decreased during the installation of Reno mattress, but then recovered shortly after completion of the installation. Abundances, richness and diversity of invertebrates and fish were found to be similar before and after the installation which suggests that the installation had no net negative impact on the site. The Reno mattresses were found to attract fauna typical of rocky shore environments as well as a few invasive alien invertebrate species. This study noted that a hybrid habitat of Reno mattress and eelgrass was created and may in fact provide the positives of both to a system. In any coastal development it will be important to balance the demands of a growing population and the protection of natural habitats. The results of this study suggest that complex artificial structures such as Reno mattresses do provide habitat for fish and invertebrates. However, the use of these structures should be in combination with natural vegetation (e.g. as a hybrid habitat) and not one that replaces intertidal and subtidal natural habitat especially eelgrass. There is limited information regarding the ecological impacts of using Reno mattresses in estuaries and this study provides new information on their ecological efficacy that should be valuable for future coastal erosion control practices.
- Full Text:
- Date Issued: 2020
Effect of the nature of nanoparticles on the photophysicochemical properties and photodynamic antimicrobial chemotherapy of phthalocyanines
- Authors: Magadla, Aviwe
- Date: 2020
- Subjects: Nanoparticles , Phthalocyanines , Anti-infective agents -- Therapeutic use , Photochemotherapy , Photochemistry
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/123107 , vital:35406
- Description: In this work, the syntheses and characterisation of Zn monocaffeic acid tri–tert–butyl phthalocyanine (1), Zn monocarboxyphenoxy tri– tert–butylphenoxyl phthalocyanine (2), tetrakis phenoxy N,N-dimethyl-4-(methylimino) phthalocyanine indium (III) chloride (3) and tetrakis N,N-dimethyl-4-(methylimino) phthalocyanine indium (III) chloride (5) are presented. Complexes 3 and 5 were further quartenised with 1,3- propanesultone to form corresponding complexes (4) and (6), respectively. Complexes 1 and 2 were covalently linked to amino functionalised nanoparticles (NPs). Complexes 3, 4, 5 and 6 where linked to oleic acid/oleylamine capped (OLA/OLM) silver-iron dimers (Ag-Fe3O4 OLA/OLM) and silver-iron core shell (Ag@Fe3O4 OLA/OLM) NPs via interaction between the nanoparticles and the imino group on the phthalocyanines. The phthalocyanine-NP conjugates afforded an increase in triplet quantum yields with a corresponding decrease in fluorescence quantum yield as compared to the phthalocyanine complexes alone. Complexes 3, 4 and their conjugates were then used for photodynamic antimicrobial chemotherapy on E. coli. The zwitterionic photosensitiser 4 and its conjugates showed better efficiency for photodynamic antimicrobial chemotherapy compared to their neutral counterparts.
- Full Text:
- Date Issued: 2020
- Authors: Magadla, Aviwe
- Date: 2020
- Subjects: Nanoparticles , Phthalocyanines , Anti-infective agents -- Therapeutic use , Photochemotherapy , Photochemistry
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/123107 , vital:35406
- Description: In this work, the syntheses and characterisation of Zn monocaffeic acid tri–tert–butyl phthalocyanine (1), Zn monocarboxyphenoxy tri– tert–butylphenoxyl phthalocyanine (2), tetrakis phenoxy N,N-dimethyl-4-(methylimino) phthalocyanine indium (III) chloride (3) and tetrakis N,N-dimethyl-4-(methylimino) phthalocyanine indium (III) chloride (5) are presented. Complexes 3 and 5 were further quartenised with 1,3- propanesultone to form corresponding complexes (4) and (6), respectively. Complexes 1 and 2 were covalently linked to amino functionalised nanoparticles (NPs). Complexes 3, 4, 5 and 6 where linked to oleic acid/oleylamine capped (OLA/OLM) silver-iron dimers (Ag-Fe3O4 OLA/OLM) and silver-iron core shell (Ag@Fe3O4 OLA/OLM) NPs via interaction between the nanoparticles and the imino group on the phthalocyanines. The phthalocyanine-NP conjugates afforded an increase in triplet quantum yields with a corresponding decrease in fluorescence quantum yield as compared to the phthalocyanine complexes alone. Complexes 3, 4 and their conjugates were then used for photodynamic antimicrobial chemotherapy on E. coli. The zwitterionic photosensitiser 4 and its conjugates showed better efficiency for photodynamic antimicrobial chemotherapy compared to their neutral counterparts.
- Full Text:
- Date Issued: 2020
Effectiveness of different interventions to reduce occupational sitting among office administrators at Rhodes University
- Authors: Malesa, Thato
- Date: 2020
- Subjects: Standing position , Sedentary behavior , Work environment , Employee health promotion , Office furniture -- Design , Industrial hygiene , Employees -- Health risk assessment , Human engineering , Compliance
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/166403 , vital:41360
- Description: Background: Sitting for prolonged periods is common in the working environment. Office workers are exposed to long periods of sitting time at work. Research has reported associations between prolonged sitting and negative health implications. As such studies have proposed different interventions aimed at reducing sitting times in the workplace. The aim of the current study was to evaluate the effectiveness of two different interventions, physical intervention (sit-stand worktables) and personal intervention (instruction to stretch) on compliance and reducing occupational sitting behaviour. The study also aimed to investigate the effectiveness of interventions in improving or impairing mood. Methods: The study took the form of a fieldbased study atRhodes University, Makhanda, South Africa. Fifteen full–time office workers in different divisions participated in the study. Participants were randomly allocated to either the physical or personal intervention group. In both intervention groups, partic ipants were prompted to stand for five minutes every hour during the workday to either work in a standing position or perform stretches. Over the course of the experiment, desk occupancy, sitting/standing time and mood effect were recorded in both intervention groups Both intervention groups were monitored for a period of three months (58 days). Results: The study found that the physical intervention group was an effective approach to reduce prolonged sitting in the workplace in comparison to the personal intervention group. Over the course of the experiment, there was sustain usage of interventions in both groups, however, in the physical intervention group desk usage decreased overtime. The findings of the study also show that some participants were more compliant with the study procedures than others. It was also found that mood improved upon introducing interventions in the workplace. However, with the outcomes of the results, the study acknowledges that several factors emerged which are likely to impact compliance, which future studies may investigate. Conclusion: Although sit-stand worktables are expensive, it seems like introducing them was successful in reducing sitting time and changing sitting behaviour in comparison to an intervention that instruct s individuals to stand up and stretch.
- Full Text:
- Date Issued: 2020
- Authors: Malesa, Thato
- Date: 2020
- Subjects: Standing position , Sedentary behavior , Work environment , Employee health promotion , Office furniture -- Design , Industrial hygiene , Employees -- Health risk assessment , Human engineering , Compliance
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/166403 , vital:41360
- Description: Background: Sitting for prolonged periods is common in the working environment. Office workers are exposed to long periods of sitting time at work. Research has reported associations between prolonged sitting and negative health implications. As such studies have proposed different interventions aimed at reducing sitting times in the workplace. The aim of the current study was to evaluate the effectiveness of two different interventions, physical intervention (sit-stand worktables) and personal intervention (instruction to stretch) on compliance and reducing occupational sitting behaviour. The study also aimed to investigate the effectiveness of interventions in improving or impairing mood. Methods: The study took the form of a fieldbased study atRhodes University, Makhanda, South Africa. Fifteen full–time office workers in different divisions participated in the study. Participants were randomly allocated to either the physical or personal intervention group. In both intervention groups, partic ipants were prompted to stand for five minutes every hour during the workday to either work in a standing position or perform stretches. Over the course of the experiment, desk occupancy, sitting/standing time and mood effect were recorded in both intervention groups Both intervention groups were monitored for a period of three months (58 days). Results: The study found that the physical intervention group was an effective approach to reduce prolonged sitting in the workplace in comparison to the personal intervention group. Over the course of the experiment, there was sustain usage of interventions in both groups, however, in the physical intervention group desk usage decreased overtime. The findings of the study also show that some participants were more compliant with the study procedures than others. It was also found that mood improved upon introducing interventions in the workplace. However, with the outcomes of the results, the study acknowledges that several factors emerged which are likely to impact compliance, which future studies may investigate. Conclusion: Although sit-stand worktables are expensive, it seems like introducing them was successful in reducing sitting time and changing sitting behaviour in comparison to an intervention that instruct s individuals to stand up and stretch.
- Full Text:
- Date Issued: 2020
Efficacy of the rind physiological disorder oleocellosis control measures in lemon fruit (Citrus limon) under shade netting
- Authors: Njili, Zamazima
- Date: 2020
- Subjects: Citrus fruits--Storage--Diseases and injuries , Citrus--Diseases and pests Citrus fruit industry Citrus products
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10948/49237 , vital:41613
- Description: Lemon growers aim to produce fruit with no blemishes on the rind. Fruit without blemishes is regarded as high value fruit required for the export market. Leakage of phytotoxic oil from the rind tissues damages them and results in the development of oleocellosis symptoms. Four experiments were conducted to investigate methods of avoiding and controlling the development of oleocellosis during harvest and post-harvest handling. The first experiment indicated that the rind oil release pressure (RORP) test is the method most effective at predicting oleocellosis incidents. The experiment also suggested that fruit not exposed to the sun are the most susceptible to oleocellosis due to low temperatures that cause high turgidity. Oleocellosis results when turgid fruit is mishandled. A cultivar experiment was carried out to ascertain the lemon cultivar most susceptible to oleocellosis. The results show that the Limoneira cultivar is the most susceptible to oleocellosis and 2PH seedless is the least susceptible cultivar when compared to other lemon cultivars such as Lisbon, Eureka and Genoa. When the use of shade netting was tested to determine its effect on oleocellosis severity, it was found that using shade netting did not have an effect. Fruit grown under shade netting had the same colour and average diameter as fruit grown in an open orchard. In the same experiment, mature fruit were shown to be more susceptible to oleocellosis than immature fruit. The final part of the project investigated harvesting and post-harvest practices to determine those that cause oleocellosis the most. It was found that fruit after degreening had greater oleocellosis due to exposure to moisture conditions and rough handling. Another practice that results in oleocellosis occurs before waxing when the fruit was roughly transferred from the bins into the packline. The third oleocellosis inducing practice was moving fruit along the conveyor belt while it is turgid. Improvement of handling procedures to reduce oleocellosis can increase export volume and result in higher value of fruit per carton.
- Full Text:
- Date Issued: 2020
- Authors: Njili, Zamazima
- Date: 2020
- Subjects: Citrus fruits--Storage--Diseases and injuries , Citrus--Diseases and pests Citrus fruit industry Citrus products
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10948/49237 , vital:41613
- Description: Lemon growers aim to produce fruit with no blemishes on the rind. Fruit without blemishes is regarded as high value fruit required for the export market. Leakage of phytotoxic oil from the rind tissues damages them and results in the development of oleocellosis symptoms. Four experiments were conducted to investigate methods of avoiding and controlling the development of oleocellosis during harvest and post-harvest handling. The first experiment indicated that the rind oil release pressure (RORP) test is the method most effective at predicting oleocellosis incidents. The experiment also suggested that fruit not exposed to the sun are the most susceptible to oleocellosis due to low temperatures that cause high turgidity. Oleocellosis results when turgid fruit is mishandled. A cultivar experiment was carried out to ascertain the lemon cultivar most susceptible to oleocellosis. The results show that the Limoneira cultivar is the most susceptible to oleocellosis and 2PH seedless is the least susceptible cultivar when compared to other lemon cultivars such as Lisbon, Eureka and Genoa. When the use of shade netting was tested to determine its effect on oleocellosis severity, it was found that using shade netting did not have an effect. Fruit grown under shade netting had the same colour and average diameter as fruit grown in an open orchard. In the same experiment, mature fruit were shown to be more susceptible to oleocellosis than immature fruit. The final part of the project investigated harvesting and post-harvest practices to determine those that cause oleocellosis the most. It was found that fruit after degreening had greater oleocellosis due to exposure to moisture conditions and rough handling. Another practice that results in oleocellosis occurs before waxing when the fruit was roughly transferred from the bins into the packline. The third oleocellosis inducing practice was moving fruit along the conveyor belt while it is turgid. Improvement of handling procedures to reduce oleocellosis can increase export volume and result in higher value of fruit per carton.
- Full Text:
- Date Issued: 2020
Elucidation of the effect of cannabinoids on ER stress to determine their molecular mechanism of action in breast cancer cells
- Authors: De la Harpe, Amy
- Date: 2020
- Subjects: Endoplasmic reticulum -- Pathophysiology , Breast -- Cancer -- Research
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10948/48213 , vital:40525
- Description: Endoplasmic reticulum (ER) stress is defined as an imbalance between the ER’s protein-folding load and folding capacity. ER stress is induced by various physiological conditions and subsequently triggers the activation of the unfolded protein response (UPR) to re-establish homeostasis and promote cell survival. However, under severe or chronic stress, apoptosis is induced. Normal cells generally do not experience ER stress; however, stressful conditions in the tumour micro-environment facilitates chronic ER stress and UPR activation, which plays a pivotal role in tumour survival. Exacerbation of pre-existing ER stress can trigger cancer cell death, with a minimal effect on normal cells. Currently, no high-throughput method exists to detect and quantify ER stress in cell lines. This study showed that Thioflavin T, a fluorescent dye that binds to misfolded protein aggregates, can be used for the high-throughput detection of ER stress, and provides several advantages over currently used methods. Current literature suggests that cannabinoid treatment may induce cancer cell death via ER stress, however little is known about the mechanism of induction. This study proposed a mechanism that occurs via the influx of Ca2+ via the vanilloid receptor 1 (TRPV1), and subsequent ROS production, which affects protein folding. ER stress was induced using various cannabinoids and measured using Thioflavin T and western blot analysis. The effect of cannabinoid treatment on ROS production and the intracellular Ca2+ concentration was measured. Cannabidiol (CBD) was the most potent ER stress inducer, significantly increasing Ca2+ and ROS accumulation; however, the level of accumulated Ca2+ across cell lines varied, which may be due to the differences in the TRPV1 expression and localization. Concomitant treatment of CBD with an antioxidant significantly increased cell viability and decreased ER stress induction in the MCF7 cell line. Concomitant treatment with a TRPV1 antagonist increased viability in this cell line. In conclusion, the results suggested that CBD may induce ER stress via Ca2+ influx through the TRPV1 receptor, thereby elevating intracellular ROS levels and disrupting protein folding.
- Full Text:
- Date Issued: 2020
- Authors: De la Harpe, Amy
- Date: 2020
- Subjects: Endoplasmic reticulum -- Pathophysiology , Breast -- Cancer -- Research
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10948/48213 , vital:40525
- Description: Endoplasmic reticulum (ER) stress is defined as an imbalance between the ER’s protein-folding load and folding capacity. ER stress is induced by various physiological conditions and subsequently triggers the activation of the unfolded protein response (UPR) to re-establish homeostasis and promote cell survival. However, under severe or chronic stress, apoptosis is induced. Normal cells generally do not experience ER stress; however, stressful conditions in the tumour micro-environment facilitates chronic ER stress and UPR activation, which plays a pivotal role in tumour survival. Exacerbation of pre-existing ER stress can trigger cancer cell death, with a minimal effect on normal cells. Currently, no high-throughput method exists to detect and quantify ER stress in cell lines. This study showed that Thioflavin T, a fluorescent dye that binds to misfolded protein aggregates, can be used for the high-throughput detection of ER stress, and provides several advantages over currently used methods. Current literature suggests that cannabinoid treatment may induce cancer cell death via ER stress, however little is known about the mechanism of induction. This study proposed a mechanism that occurs via the influx of Ca2+ via the vanilloid receptor 1 (TRPV1), and subsequent ROS production, which affects protein folding. ER stress was induced using various cannabinoids and measured using Thioflavin T and western blot analysis. The effect of cannabinoid treatment on ROS production and the intracellular Ca2+ concentration was measured. Cannabidiol (CBD) was the most potent ER stress inducer, significantly increasing Ca2+ and ROS accumulation; however, the level of accumulated Ca2+ across cell lines varied, which may be due to the differences in the TRPV1 expression and localization. Concomitant treatment of CBD with an antioxidant significantly increased cell viability and decreased ER stress induction in the MCF7 cell line. Concomitant treatment with a TRPV1 antagonist increased viability in this cell line. In conclusion, the results suggested that CBD may induce ER stress via Ca2+ influx through the TRPV1 receptor, thereby elevating intracellular ROS levels and disrupting protein folding.
- Full Text:
- Date Issued: 2020
Evaluation of an NADPH-dependent assay for inhibition screening of Salmonella enterica DOXP Reguctoisomerase for identification of novel drug hit compounds
- Authors: Ngcongco, Khanyisile
- Date: 2020
- Subjects: 1-Deoxy-D-xylulose 5-phosphate , Antibiotics , Drug development , Salmonella , Enterobacteriaceae , Vaccines , Plasmodium falciparum , Mycobacterium tuberculosis
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/167132 , vital:41440
- Description: Invasive non-typhoidal Salmonella, caused by the intracellular pathogen Salmonella enterica, has emerged as a major cause of bloodstream infections. It remains a neglected infection responsible for many deaths in Africa, as it fails to receive the level of support that is given to most better known infections. There are currently no vaccines against invasive non-typhoidal Salmonella. First-line antibiotics have been used for treatment, however, the rise in the resistance of the bacteria against these antibiotics has made treatment of invasive salmonellosis into a clinical problem. Therefore, the discovery of new compounds for the development of antibiotic drugs is required. Central metabolic pathways can be a useful source for identifying drug targets and among these is the non-mevalonate pathway, one of the pathways used for the biosynthesis of isoprenoid precursors. The second step of the non-mevalonate pathway involves the NADPH-dependent reduction of 1-deoxy-D-xylulose 5-phosphate (DOXP) into 2-C-methyl-D-erythritol 4-phosphate (MEP). 1-Deoxy-D-xylulose 5-phosphate (DOXP) reductoisomerase plays a vital role in the catalysis of this reaction and requires NADPH and divalent metal cations as co-factors for its activity. In this investigation recombinant DOXP reductoisomerase from Salmonella enterica, Plasmodium falciparum and Mycobacterium tuberculosis were biochemically characterized as potential targets for developing drugs that could be used as treatment of the disease. The expression and nickel-chelate affinity purification of S. enterica DOXP reductoisomerase in a fully functional native state was successfully achieved. However, the expression and purification of P. falciparum DXR and M. tuberculosis DXR was unsuccessful due to the formation of insoluble inclusion bodies. Although alternative purification strategies were explored, including dialysis and slow dilution, these proteins remained insoluble, making their functional analysis not possible. An NADPH-dependent enzyme assay was used to determine the activity of S. enterica DXR. This assay monitors the reduction of DOXP to MEP by measuring the absorbance at 340 nm, which reflects the concentration of NADPH. An alternative assay, resazurin reduction, which monitors the NADPH-dependent reduction of resazurin to resorufin, was explored for detecting enzyme activity. The recombinant S. enterica DOXP reductoisomerase had a specific activity of 0.126 ± 0.0014 μmol/min/mg protein and a Km and Vmax of 881 μM and 0.249 μmol/min/mg respectively. FR900098, a derivative of fosmidomycin, is a well-known inhibitor of DXR, however, the sensitivity of S. enterica DXR towards FR900098 has not yet been reported. The NADPH dependent enzyme and resazurin reduction assays were used to determine whether FR900098 has enzyme inhibitory effects against S. enterica DXR. Upon confirming that FR900098 is able to inhibit S. enterica DXR, FR900098 was used as a control compound in the screening of novel compounds. The S. enterica DXR enzyme was screened for inhibition by the collection of compounds from the Pathogen Box. Compounds that exhibited the desired inhibitory activity, referred to as ‘hits’ were selected for further investigation. These hits were confirmed using the NADPH-dependent enzyme assay, resulting in the identification of two different DXR inhibitor compounds, MMV002816, also known as diethylcarbamazine, and MMV228911. The inhibitory concentration (IC50) values of FR900098, MMV002816 and MMV228911 against S. enterica DXR were 1.038 μM, 2.173 μM and 6.861 μM respectively. The binding mode of these compounds to S. enterica DXR could lead to the discovery of novel druggable sites on the enzyme and stimulate the development of new antibiotics that can be used for treating Salmonella infections.
- Full Text:
- Date Issued: 2020
- Authors: Ngcongco, Khanyisile
- Date: 2020
- Subjects: 1-Deoxy-D-xylulose 5-phosphate , Antibiotics , Drug development , Salmonella , Enterobacteriaceae , Vaccines , Plasmodium falciparum , Mycobacterium tuberculosis
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/167132 , vital:41440
- Description: Invasive non-typhoidal Salmonella, caused by the intracellular pathogen Salmonella enterica, has emerged as a major cause of bloodstream infections. It remains a neglected infection responsible for many deaths in Africa, as it fails to receive the level of support that is given to most better known infections. There are currently no vaccines against invasive non-typhoidal Salmonella. First-line antibiotics have been used for treatment, however, the rise in the resistance of the bacteria against these antibiotics has made treatment of invasive salmonellosis into a clinical problem. Therefore, the discovery of new compounds for the development of antibiotic drugs is required. Central metabolic pathways can be a useful source for identifying drug targets and among these is the non-mevalonate pathway, one of the pathways used for the biosynthesis of isoprenoid precursors. The second step of the non-mevalonate pathway involves the NADPH-dependent reduction of 1-deoxy-D-xylulose 5-phosphate (DOXP) into 2-C-methyl-D-erythritol 4-phosphate (MEP). 1-Deoxy-D-xylulose 5-phosphate (DOXP) reductoisomerase plays a vital role in the catalysis of this reaction and requires NADPH and divalent metal cations as co-factors for its activity. In this investigation recombinant DOXP reductoisomerase from Salmonella enterica, Plasmodium falciparum and Mycobacterium tuberculosis were biochemically characterized as potential targets for developing drugs that could be used as treatment of the disease. The expression and nickel-chelate affinity purification of S. enterica DOXP reductoisomerase in a fully functional native state was successfully achieved. However, the expression and purification of P. falciparum DXR and M. tuberculosis DXR was unsuccessful due to the formation of insoluble inclusion bodies. Although alternative purification strategies were explored, including dialysis and slow dilution, these proteins remained insoluble, making their functional analysis not possible. An NADPH-dependent enzyme assay was used to determine the activity of S. enterica DXR. This assay monitors the reduction of DOXP to MEP by measuring the absorbance at 340 nm, which reflects the concentration of NADPH. An alternative assay, resazurin reduction, which monitors the NADPH-dependent reduction of resazurin to resorufin, was explored for detecting enzyme activity. The recombinant S. enterica DOXP reductoisomerase had a specific activity of 0.126 ± 0.0014 μmol/min/mg protein and a Km and Vmax of 881 μM and 0.249 μmol/min/mg respectively. FR900098, a derivative of fosmidomycin, is a well-known inhibitor of DXR, however, the sensitivity of S. enterica DXR towards FR900098 has not yet been reported. The NADPH dependent enzyme and resazurin reduction assays were used to determine whether FR900098 has enzyme inhibitory effects against S. enterica DXR. Upon confirming that FR900098 is able to inhibit S. enterica DXR, FR900098 was used as a control compound in the screening of novel compounds. The S. enterica DXR enzyme was screened for inhibition by the collection of compounds from the Pathogen Box. Compounds that exhibited the desired inhibitory activity, referred to as ‘hits’ were selected for further investigation. These hits were confirmed using the NADPH-dependent enzyme assay, resulting in the identification of two different DXR inhibitor compounds, MMV002816, also known as diethylcarbamazine, and MMV228911. The inhibitory concentration (IC50) values of FR900098, MMV002816 and MMV228911 against S. enterica DXR were 1.038 μM, 2.173 μM and 6.861 μM respectively. The binding mode of these compounds to S. enterica DXR could lead to the discovery of novel druggable sites on the enzyme and stimulate the development of new antibiotics that can be used for treating Salmonella infections.
- Full Text:
- Date Issued: 2020
Evaluation of Baited Remote Underwater Video Systems (BRUVS) for monitoring fish communities in Lake Malawi/Niassa
- Authors: Van Wyk, Angus
- Date: 2020
- Subjects: Underwater videography -- Malawi , Chambo -- Malawi -- Monitoring , Fishes -- Malawi -- Monitoring , Oreochromis lidole -- Malawi -- Monitoring
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/145546 , vital:38448
- Description: Baited Remote Underwater Video systems (BRUVS) have become a well-established in-situ monitoring technique in clearwater aquatic ecosystems. The relatively low cost, nondestructive and non-extractive nature of this technique permits BRUVS to be employed in a wide range of habitats. To date, the vast majority of studies using BRUVS technology have been conducted in marine habitats. Subsequently, BRUVS applications in freshwater habitats are scant, and techniques are not well developed. The primary objective of this thesis was to address this knowledge gap and develop standard operating procedures for BRUVS employment in Lake Malawi/Niassa and explore the potential of BRUVS as a monitoring tool for fish communities in the African Great Lakes. Eight easily identifiable species groups, representative of Lake Malawi/Niassa inshore fish communities, were used to develop the technique. The optimal BRUVS deployment time to obtain 95 % species accumulation was achieved in a 15-minute recording period. Power analysis, using a pre-determined 80 % power, a confidence interval of 95 % and a significance level of < 0.05 was used to determine annual sampling effort requirements for each species group. The power analysis was performed to detect a 10 % change in abundance over a hypothetical 10-year monitoring scenario. In areas where fish abundance was lower, the sampling effort required to monitor key fisheries species was significantly higher. For example, Chambo, the local Oreochromis (Nyasalapia) species flock, required an annual sampling effort of 120 deployments in Malawi compared to 56 in Mozambique ( < 0.05). Chambo had a higher detection probability in areas of lesser fishing pressure and were found in higher abundances in deeper, less accessible habitats. Deep-water (> 20 m) and rocky habitats were most important in explaining Chambo abundance and detection probability. The size-structure of Chambo in Lake Malawi/Niassa reflects size-specific depth and habitat migrations. Larger Chambo were observed aggregating in waters deeper than 20 m and a broader size range of individuals were observed utilising structured habitat. The effects of fishing are apparent in the size-structure of Chambo in the areas sampled. In study areas exposed to greater levels of fishing pressure – such as Malawi, the BRUVS detected significantly fewer individuals within sexually mature size classes, and the average size was smaller than in areas with less exploitation.
- Full Text:
- Date Issued: 2020
- Authors: Van Wyk, Angus
- Date: 2020
- Subjects: Underwater videography -- Malawi , Chambo -- Malawi -- Monitoring , Fishes -- Malawi -- Monitoring , Oreochromis lidole -- Malawi -- Monitoring
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/145546 , vital:38448
- Description: Baited Remote Underwater Video systems (BRUVS) have become a well-established in-situ monitoring technique in clearwater aquatic ecosystems. The relatively low cost, nondestructive and non-extractive nature of this technique permits BRUVS to be employed in a wide range of habitats. To date, the vast majority of studies using BRUVS technology have been conducted in marine habitats. Subsequently, BRUVS applications in freshwater habitats are scant, and techniques are not well developed. The primary objective of this thesis was to address this knowledge gap and develop standard operating procedures for BRUVS employment in Lake Malawi/Niassa and explore the potential of BRUVS as a monitoring tool for fish communities in the African Great Lakes. Eight easily identifiable species groups, representative of Lake Malawi/Niassa inshore fish communities, were used to develop the technique. The optimal BRUVS deployment time to obtain 95 % species accumulation was achieved in a 15-minute recording period. Power analysis, using a pre-determined 80 % power, a confidence interval of 95 % and a significance level of < 0.05 was used to determine annual sampling effort requirements for each species group. The power analysis was performed to detect a 10 % change in abundance over a hypothetical 10-year monitoring scenario. In areas where fish abundance was lower, the sampling effort required to monitor key fisheries species was significantly higher. For example, Chambo, the local Oreochromis (Nyasalapia) species flock, required an annual sampling effort of 120 deployments in Malawi compared to 56 in Mozambique ( < 0.05). Chambo had a higher detection probability in areas of lesser fishing pressure and were found in higher abundances in deeper, less accessible habitats. Deep-water (> 20 m) and rocky habitats were most important in explaining Chambo abundance and detection probability. The size-structure of Chambo in Lake Malawi/Niassa reflects size-specific depth and habitat migrations. Larger Chambo were observed aggregating in waters deeper than 20 m and a broader size range of individuals were observed utilising structured habitat. The effects of fishing are apparent in the size-structure of Chambo in the areas sampled. In study areas exposed to greater levels of fishing pressure – such as Malawi, the BRUVS detected significantly fewer individuals within sexually mature size classes, and the average size was smaller than in areas with less exploitation.
- Full Text:
- Date Issued: 2020
Exploring Quinolinyl-Thiazolidinedione hybrid compounds as potential anti-tubercular agents
- Authors: Mtshare, Thanduxolo Elihle
- Date: 2020
- Subjects: Quinoline , Mycobacterium tuberculosis , Tuberculosis -- Chemotherapy , Plasmodium falciparum , Thiazolidinedione
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/143314 , vital:38232
- Description: Tuberculosis (TB) is an infectious disease caused by the pathogen, Mycobacterium tuberculosis. According to the World Health Organization, TB is the ninth leading cause of death worldwide ranking above HIV/AIDS. This high mortality rate of TB begs the questions about the efficiency of the current therapy and raises an urgent need to create novel anti-tuberculosis agents which will aid in curbing this burden. Quinoline containing compounds have remarkable biological activities across a wide spectrum of diseases including anti-tuberculosis. On the other hand, thiazolidinedione containing compounds possess a broad spectrum of biological properties. In this study, we rationally designed compounds containing these pharmacophoric units and investigated them for their potential biological activity against Mycobacterium tuberculosis. Considering antimalarial activity of quinoline-based compounds, the compounds achieved were also cross-screened for their activity against the Plasmodium falciparum parasite, a causative agent of malaria. In all the synthesized compounds, compound 2.6a, 2.6b and 2.7b emerged as most active compounds against the H37Rv strain with MIC₉₀ values ranging in between of 1.08 – 17.1 μM. In addition, none of the compounds showed any inhibitory activities against the 3D7 strain of P. falciparum parasite. All the compounds prepared in this study showed no significant human cytotoxic effects as measured by HeLa cell line.
- Full Text:
- Date Issued: 2020
- Authors: Mtshare, Thanduxolo Elihle
- Date: 2020
- Subjects: Quinoline , Mycobacterium tuberculosis , Tuberculosis -- Chemotherapy , Plasmodium falciparum , Thiazolidinedione
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/143314 , vital:38232
- Description: Tuberculosis (TB) is an infectious disease caused by the pathogen, Mycobacterium tuberculosis. According to the World Health Organization, TB is the ninth leading cause of death worldwide ranking above HIV/AIDS. This high mortality rate of TB begs the questions about the efficiency of the current therapy and raises an urgent need to create novel anti-tuberculosis agents which will aid in curbing this burden. Quinoline containing compounds have remarkable biological activities across a wide spectrum of diseases including anti-tuberculosis. On the other hand, thiazolidinedione containing compounds possess a broad spectrum of biological properties. In this study, we rationally designed compounds containing these pharmacophoric units and investigated them for their potential biological activity against Mycobacterium tuberculosis. Considering antimalarial activity of quinoline-based compounds, the compounds achieved were also cross-screened for their activity against the Plasmodium falciparum parasite, a causative agent of malaria. In all the synthesized compounds, compound 2.6a, 2.6b and 2.7b emerged as most active compounds against the H37Rv strain with MIC₉₀ values ranging in between of 1.08 – 17.1 μM. In addition, none of the compounds showed any inhibitory activities against the 3D7 strain of P. falciparum parasite. All the compounds prepared in this study showed no significant human cytotoxic effects as measured by HeLa cell line.
- Full Text:
- Date Issued: 2020
Finite precision arithmetic in Polyphase Filterbank implementations
- Authors: Myburgh, Talon
- Date: 2020
- Subjects: Radio interferometers , Interferometry , Radio telescopes , Gate array circuits , Floating-point arithmetic , Python (Computer program language) , Polyphase Filterbank , Finite precision arithmetic , MeerKAT
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/146187 , vital:38503
- Description: The MeerKAT is the most sensitive radio telescope in its class, and it is important that systematic effects do not limit the dynamic range of the instrument, preventing this sensitivity from being harnessed for deep integrations. During commissioning, spurious artefacts were noted in the MeerKAT passband and the root cause was attributed to systematic errors in the digital signal path. Finite precision arithmetic used by the Polyphase Filterbank (PFB) was one of the main factors contributing to the spurious responses, together with bugs in the firmware. This thesis describes a software PFB simulator that was built to mimic the MeerKAT PFB and allow investigation into the origin and mitigation of the effects seen on the telescope. This simulator was used to investigate the effects in signal integrity of various rounding techniques, overflow strategies and dual polarisation processing in the PFB. Using the simulator to investigate a number of different signal levels, bit-width and algorithmic scenarios, it gave insight into how the periodic dips occurring in the MeerKAT passband were the result of the implementation using an inappropriate rounding strategy. It further indicated how to select the best strategy for preventing overflow while maintaining high quantization effciency in the FFT. This practice of simulating the design behaviour in the PFB independently of the tools used to design the DSP firmware, is a step towards an end-to-end simulation of the MeerKAT system (or any radio telescope using nite precision digital signal processing systems). This would be useful for design, diagnostics, signal analysis and prototyping of the overall instrument.
- Full Text:
- Date Issued: 2020
- Authors: Myburgh, Talon
- Date: 2020
- Subjects: Radio interferometers , Interferometry , Radio telescopes , Gate array circuits , Floating-point arithmetic , Python (Computer program language) , Polyphase Filterbank , Finite precision arithmetic , MeerKAT
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/146187 , vital:38503
- Description: The MeerKAT is the most sensitive radio telescope in its class, and it is important that systematic effects do not limit the dynamic range of the instrument, preventing this sensitivity from being harnessed for deep integrations. During commissioning, spurious artefacts were noted in the MeerKAT passband and the root cause was attributed to systematic errors in the digital signal path. Finite precision arithmetic used by the Polyphase Filterbank (PFB) was one of the main factors contributing to the spurious responses, together with bugs in the firmware. This thesis describes a software PFB simulator that was built to mimic the MeerKAT PFB and allow investigation into the origin and mitigation of the effects seen on the telescope. This simulator was used to investigate the effects in signal integrity of various rounding techniques, overflow strategies and dual polarisation processing in the PFB. Using the simulator to investigate a number of different signal levels, bit-width and algorithmic scenarios, it gave insight into how the periodic dips occurring in the MeerKAT passband were the result of the implementation using an inappropriate rounding strategy. It further indicated how to select the best strategy for preventing overflow while maintaining high quantization effciency in the FFT. This practice of simulating the design behaviour in the PFB independently of the tools used to design the DSP firmware, is a step towards an end-to-end simulation of the MeerKAT system (or any radio telescope using nite precision digital signal processing systems). This would be useful for design, diagnostics, signal analysis and prototyping of the overall instrument.
- Full Text:
- Date Issued: 2020
Flammability of indigenous and invasive alien woody plants in coastal fynbos and thicket
- Msweli, Samukelisiwe Tholakele, Potts, Alastair J
- Authors: Msweli, Samukelisiwe Tholakele , Potts, Alastair J
- Date: 2020
- Subjects: Forest fires -- Environmental aspects , Forest fires -- Prevention and control
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10948/45875 , vital:39313
- Description: Globally, extreme fires have become more common in wildland-urban interface areas, and more recently, along the southern Cape coast of South Africa. The 2017 Knysna fires prompted greater understanding of the flammability of, and the fire risk posed by, different vegetation groups, which is essential to develop fire risk mitigation strategies. In this study, I experimentally assessed flammability of 30 woody plant species from the vegetation groups indigenous fynbos, thicket, and invasive alien plants (IAPs) that occur along the southern Cape coast. Live plant shoots were sampled across varying fire weather conditions and burnt experimentally to measure flammability in relation to fire weather conditions, fuel moisture, and fuel load. Flammability measures considered were: burn intensity, completeness of burn, time-to-ignition, and the likelihood of spontaneous ignition. I further assessed the flammability of partially dried plant material as a crude proxy for drought effects, to ascertain whether drying of fuels would differentially affect the flammability of the vegetation groups. I used generalized linear mixed-effects models to assess flammability measures in relation to the predictor variables: fire weather, fuel moisture, fuel load, vegetation groups, and species (as a random factor). Results showed that increasing severity of fire weather significantly increased flammability through increasing burn intensity, increasing completeness of burn, increasing the likelihood of spontaneous ignition, and also reducing time-to-ignition. Increasing fuel moisture significantly decreased burn intensity, completeness of burn, and the likelihood of spontaneous ignition. Fuel load significantly increased burn intensity and time-to-ignition. Flammability was highest in IAPs, intermediate in fynbos, and lowest in thicket. IAPs and fynbos showed significantly higher ignitability, and thus present risks under moderate and high fire weather conditions, whereas thicket presents lower risks under low and moderate fire weather conditions. The drying out of fuels considerably increased flammability equally in the three vegetation groups, and by implication, fire risk due to an increase in dead:live ratio. Flammability was furthermore assessed in relation to fuel traits, i.e. the proportion of fine fuels, coarse fuels, and dead fuels, fuel bed porosity, fuel load, and fuel moisture, using multiple regression analysis and stepwise selection of factors. This revealed that fuel moisture was the most important factor affecting flammability in terms of all the flammability measures. Results further showed that the increase in v the proportions of fine fuels increased flammability by increasing completeness of burn. Lastly, vegetation groups were compared (using Kruskal Wallis) in terms of their flammability and fuel traits. I found that fynbos and IAPs exhibited greater flammability on account of higher completeness of burn and more rapid ignition than thicket species, but no clear distinction was evident between fynbos and IAPs. Fynbos’ high flammability was attributed to high proportions of fine and porous fuels. Thicket’s low flammability was attributed to high proportions of coarse and dense fuels. Little distinction in fuel traits could be made between fynbos and IAPs, except that fynbos had a greater proportion of fine fuels. There is a potential risk posed by the IAPs in terms of increased flammability and fire severity, on an indigenous landscape that is invaded. Fire managers need to encourage the prioritization of the management of IAPs that present high flammability as an attempt to reduce fire risk along the southern Cape coast of South Africa.
- Full Text:
- Date Issued: 2020
- Authors: Msweli, Samukelisiwe Tholakele , Potts, Alastair J
- Date: 2020
- Subjects: Forest fires -- Environmental aspects , Forest fires -- Prevention and control
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10948/45875 , vital:39313
- Description: Globally, extreme fires have become more common in wildland-urban interface areas, and more recently, along the southern Cape coast of South Africa. The 2017 Knysna fires prompted greater understanding of the flammability of, and the fire risk posed by, different vegetation groups, which is essential to develop fire risk mitigation strategies. In this study, I experimentally assessed flammability of 30 woody plant species from the vegetation groups indigenous fynbos, thicket, and invasive alien plants (IAPs) that occur along the southern Cape coast. Live plant shoots were sampled across varying fire weather conditions and burnt experimentally to measure flammability in relation to fire weather conditions, fuel moisture, and fuel load. Flammability measures considered were: burn intensity, completeness of burn, time-to-ignition, and the likelihood of spontaneous ignition. I further assessed the flammability of partially dried plant material as a crude proxy for drought effects, to ascertain whether drying of fuels would differentially affect the flammability of the vegetation groups. I used generalized linear mixed-effects models to assess flammability measures in relation to the predictor variables: fire weather, fuel moisture, fuel load, vegetation groups, and species (as a random factor). Results showed that increasing severity of fire weather significantly increased flammability through increasing burn intensity, increasing completeness of burn, increasing the likelihood of spontaneous ignition, and also reducing time-to-ignition. Increasing fuel moisture significantly decreased burn intensity, completeness of burn, and the likelihood of spontaneous ignition. Fuel load significantly increased burn intensity and time-to-ignition. Flammability was highest in IAPs, intermediate in fynbos, and lowest in thicket. IAPs and fynbos showed significantly higher ignitability, and thus present risks under moderate and high fire weather conditions, whereas thicket presents lower risks under low and moderate fire weather conditions. The drying out of fuels considerably increased flammability equally in the three vegetation groups, and by implication, fire risk due to an increase in dead:live ratio. Flammability was furthermore assessed in relation to fuel traits, i.e. the proportion of fine fuels, coarse fuels, and dead fuels, fuel bed porosity, fuel load, and fuel moisture, using multiple regression analysis and stepwise selection of factors. This revealed that fuel moisture was the most important factor affecting flammability in terms of all the flammability measures. Results further showed that the increase in v the proportions of fine fuels increased flammability by increasing completeness of burn. Lastly, vegetation groups were compared (using Kruskal Wallis) in terms of their flammability and fuel traits. I found that fynbos and IAPs exhibited greater flammability on account of higher completeness of burn and more rapid ignition than thicket species, but no clear distinction was evident between fynbos and IAPs. Fynbos’ high flammability was attributed to high proportions of fine and porous fuels. Thicket’s low flammability was attributed to high proportions of coarse and dense fuels. Little distinction in fuel traits could be made between fynbos and IAPs, except that fynbos had a greater proportion of fine fuels. There is a potential risk posed by the IAPs in terms of increased flammability and fire severity, on an indigenous landscape that is invaded. Fire managers need to encourage the prioritization of the management of IAPs that present high flammability as an attempt to reduce fire risk along the southern Cape coast of South Africa.
- Full Text:
- Date Issued: 2020
Fluid characteristics in hydrothermal veins of the Twangiza-Namoya Gold Belt, South Kivu and Maniema Provinces, DRC
- Authors: Reid, Wesson Kyle
- Date: 2020
- Subjects: Gold ores -- Geology -- Kivu (Congo : Region) , Mineralogy -- Congo (Democratic Republic) , Hydrothermal deposits -- Congo (Democratic Republic) , Quartz -- Congo (Democratic Republic)
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/167314 , vital:41467
- Description: This study evaluates fluid variations in hydrothermal quartz veins from gold deposits in Kamiuga, Lugushwa and Namoya, located in the Twangiza-Namoya Gold Belt (TNGB) of the Kibara Belt in the eastern Democratic Republic of the Congo (DRC. Petrographic, fluid inclusion (FI) microthermometric observations and Raman spectroscopy provided qualitative and quantitative fluid composition data on the hydrothermal and magmatic fluids and their evolution during mineral precipitation. The formational fluids, based on genetically specific characteristics, were categorized into six distinct FI Types. Type 1 to 4 FIs are common in all TNGB fluids. Type 1 and 2 FIs are high salinity halite bearing FIs that indicate formation fluids that are predominantly metamorphic-sedimentary in source. CO2 vapour-bearing and the aqueous-saline CO2 liquid-bearing Type 3 FIs commonly contain CH4 and/or N2. Type 4 FIs are saline aqueous and commonly co-genetic with Type 1 and 2 FIs. Type 5 CO2-rich FIs contain either sulphide crystals, amorphous or crystalline carbon. Type 3 and 5 FIs indicate fluid sources rich in organic materials. Type 6 single aqueous-liquid phase FIs have no apour bubble, lacked a visible phase change on heating and were not thermometrically evaluated. The data indicated a high correlation between fluid composition and gold grades. High Au grade veins correlate with CO2 bearing Type 3 and 5 FIs -predominantly liquid-bearing CO2 fluids and quartz veins and fluids that contain increased organic material and sulphides. The polyphase quartz veins show highly variable homogenisation and formational temperatures exceeding 400°C. Formation conditions indicate high trapping temperatures in relation to the pressures at which fluids were captured. The high depth-temperature gradients are likely associated with mesothermal orogenic go ld deposition. Mineralisation is interpreted to have taken place as a result of mobilisation of fluids during the Pan African orogeny. Based on fluid petrography and microthermometry, gold mineralisation is most likely associated with secondary fluid influx from metamorphic sedimentary sourrces such as metapelites. The correlation between high gold grades and secondary fluids containing sulphides, high depth-temperature gradients, elevated CO2, CH4 and organic materials suggest black shales as a possible primary fluid and gold source. The development of variable and multiple fluid influx events and interactions with host rocks and imported materials resulted in complex polyphase quartz veins; the product of which created viable gold deposits throughout the TNGB. The six FI Types provides evidence of the diversity in the formation and evolution of gold deposits in the TNGB.
- Full Text:
- Date Issued: 2020
- Authors: Reid, Wesson Kyle
- Date: 2020
- Subjects: Gold ores -- Geology -- Kivu (Congo : Region) , Mineralogy -- Congo (Democratic Republic) , Hydrothermal deposits -- Congo (Democratic Republic) , Quartz -- Congo (Democratic Republic)
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/167314 , vital:41467
- Description: This study evaluates fluid variations in hydrothermal quartz veins from gold deposits in Kamiuga, Lugushwa and Namoya, located in the Twangiza-Namoya Gold Belt (TNGB) of the Kibara Belt in the eastern Democratic Republic of the Congo (DRC. Petrographic, fluid inclusion (FI) microthermometric observations and Raman spectroscopy provided qualitative and quantitative fluid composition data on the hydrothermal and magmatic fluids and their evolution during mineral precipitation. The formational fluids, based on genetically specific characteristics, were categorized into six distinct FI Types. Type 1 to 4 FIs are common in all TNGB fluids. Type 1 and 2 FIs are high salinity halite bearing FIs that indicate formation fluids that are predominantly metamorphic-sedimentary in source. CO2 vapour-bearing and the aqueous-saline CO2 liquid-bearing Type 3 FIs commonly contain CH4 and/or N2. Type 4 FIs are saline aqueous and commonly co-genetic with Type 1 and 2 FIs. Type 5 CO2-rich FIs contain either sulphide crystals, amorphous or crystalline carbon. Type 3 and 5 FIs indicate fluid sources rich in organic materials. Type 6 single aqueous-liquid phase FIs have no apour bubble, lacked a visible phase change on heating and were not thermometrically evaluated. The data indicated a high correlation between fluid composition and gold grades. High Au grade veins correlate with CO2 bearing Type 3 and 5 FIs -predominantly liquid-bearing CO2 fluids and quartz veins and fluids that contain increased organic material and sulphides. The polyphase quartz veins show highly variable homogenisation and formational temperatures exceeding 400°C. Formation conditions indicate high trapping temperatures in relation to the pressures at which fluids were captured. The high depth-temperature gradients are likely associated with mesothermal orogenic go ld deposition. Mineralisation is interpreted to have taken place as a result of mobilisation of fluids during the Pan African orogeny. Based on fluid petrography and microthermometry, gold mineralisation is most likely associated with secondary fluid influx from metamorphic sedimentary sourrces such as metapelites. The correlation between high gold grades and secondary fluids containing sulphides, high depth-temperature gradients, elevated CO2, CH4 and organic materials suggest black shales as a possible primary fluid and gold source. The development of variable and multiple fluid influx events and interactions with host rocks and imported materials resulted in complex polyphase quartz veins; the product of which created viable gold deposits throughout the TNGB. The six FI Types provides evidence of the diversity in the formation and evolution of gold deposits in the TNGB.
- Full Text:
- Date Issued: 2020
Formulation and evaluation of liposomal films for buccal delivery of antiretroviral drug
- Authors: Okafor, Nnamdi Ikemefuna
- Date: 2020
- Subjects: Liposomes , Highly active antiretroviral therapy , Antiretroviral agents , HIV infections -- Prevention
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/117161 , vital:34485
- Description: The human immune deficiency virus (HIV) infection has been ranked as one of the most devastating microbial infections in the world. This status is a result of the HIV rapid genetic variation, which limits discovery of a vaccine. Use application of antiretroviral therapy (ARVT) in treatment of the disease caused by the HIV infection (known as acquired immunodeficiency syndrome, HIV-AIDS) is frequently compromised by several factors such as the low bioavailability and severe adverse effects associated with the existing antiretroviral drugs (ARVDs). This underlines the need for controlling the pharmacokinetics profiles of ARVD using effective vehicles that can modify drug biodistribution. The same is true for many other conditions, where delivery systems can determine the success or failure of treatment by controlling pharmacokinetic and dynamic properties. The mucosal linings of the oral cavities in addition offer adorable route of administration for systematic drug delivery, improving drug therapeutic performance and often preferred by clinicians and patients. Liposomes are tiny spherical sacs of phospholipid molecules enclosing water droplets, formed (artificially) to carry drugs or other substances into the tissues by crossing and targeting to specific organelles. This work therefore focused on preparation of liposomes and liposomal buccal films (BFs) for potential buccal delivery of efavirenz, an ARVD model endowed with poor solubility and several side effects. The liposomes were prepared by thin film hydration method using crude soybean lecithin (CL) and cholesterol. Efavirenz loaded liposomes were evaluated for particle size, Zeta potential (ZP), morphology, encapsulation efficiency (EE%) and release kinetics studies. The physiochemical properties of the liposomes were also evaluated using Differential Scanning Calorimetry (DSC), X-ray diffraction (XRD), energy dispersity spectroscopy (EDS), and Fourier transform infrared (FTIR), while the formulation with the best encapsulation efficiency was used as the solvent medium for the buccal film formation. The buccal films were prepared using solvent casting method, where the liposomal suspension was used as the dispersing medium. The films were optimized for physical properties (thickness, weight variation and folding endurance) using digital Vernier calliper and digital weighing balance. The physiochemical properties of the selected BFs films made of Carbopol (CP) and its combination with Pluronic F127 (PF127) were further characterized using XRD, DSC, FTIR, Transmission Electron Microscopy (TEM), EDS and Scanning Electron Microscopy (SEM). The permeation study of the selected BFs was investigated using Franz diffusion cell. The BFs composed of CP alone or its combination with PF127 demonstrated much better bio-adhesive properties than the films made of other polymers (like Hydroxyl propyl methyl cellulose, HPMC) alone or in combination with PF127. The developed liposome formulation showed high encapsulation 98.8 ± 0.01 % in CL to cholesterol mass ratio of 1:1 and total lipid to drug mass ratio of 2:1. The average particle size 104.82 ± 2.29 nm and Zeta potential -50.33 ± 0.95 mV of these liposomes were found to be attractive for targeted delivery to the HIV infected cells. The CP based BFs (without and with PF127) exhibited good film thickness 0.88 ± 0.10 and 0.76 ± 0.14 mm, with weight uniformity 68.22 ± 1.04 and 86.28 ± 2. 16 mg, satisfactory flexibility values 258 and 321, and slightly acidic pH 6.43 ± 0.76 and 6.32 ± 0.01. The swelling percentage was found to be 50 % for CP film alone and 78 % for CP film with PF127. The cumulative amount of drug that permeated through the buccal epithelium over 24 hours was about 66 % from CP film alone and 75 % from CP film with PF127. Since no evidence of the liposomal encapsulation of EFV have been reported to our knowledge, we find the insights from the present study valuable as a set of preliminary data to encourage further investigations of the encapsulation and delivery of EFV like antiretrovirals for enhanced solubility, site targeting and prolonged release using crude soybean lecithin and mucoadhesive polymers, which holds some added economical values as naturally occurring lipid and polymeric mixtures as a promising delivery systems for buccal delivery of ARVDs.
- Full Text:
- Date Issued: 2020
- Authors: Okafor, Nnamdi Ikemefuna
- Date: 2020
- Subjects: Liposomes , Highly active antiretroviral therapy , Antiretroviral agents , HIV infections -- Prevention
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/117161 , vital:34485
- Description: The human immune deficiency virus (HIV) infection has been ranked as one of the most devastating microbial infections in the world. This status is a result of the HIV rapid genetic variation, which limits discovery of a vaccine. Use application of antiretroviral therapy (ARVT) in treatment of the disease caused by the HIV infection (known as acquired immunodeficiency syndrome, HIV-AIDS) is frequently compromised by several factors such as the low bioavailability and severe adverse effects associated with the existing antiretroviral drugs (ARVDs). This underlines the need for controlling the pharmacokinetics profiles of ARVD using effective vehicles that can modify drug biodistribution. The same is true for many other conditions, where delivery systems can determine the success or failure of treatment by controlling pharmacokinetic and dynamic properties. The mucosal linings of the oral cavities in addition offer adorable route of administration for systematic drug delivery, improving drug therapeutic performance and often preferred by clinicians and patients. Liposomes are tiny spherical sacs of phospholipid molecules enclosing water droplets, formed (artificially) to carry drugs or other substances into the tissues by crossing and targeting to specific organelles. This work therefore focused on preparation of liposomes and liposomal buccal films (BFs) for potential buccal delivery of efavirenz, an ARVD model endowed with poor solubility and several side effects. The liposomes were prepared by thin film hydration method using crude soybean lecithin (CL) and cholesterol. Efavirenz loaded liposomes were evaluated for particle size, Zeta potential (ZP), morphology, encapsulation efficiency (EE%) and release kinetics studies. The physiochemical properties of the liposomes were also evaluated using Differential Scanning Calorimetry (DSC), X-ray diffraction (XRD), energy dispersity spectroscopy (EDS), and Fourier transform infrared (FTIR), while the formulation with the best encapsulation efficiency was used as the solvent medium for the buccal film formation. The buccal films were prepared using solvent casting method, where the liposomal suspension was used as the dispersing medium. The films were optimized for physical properties (thickness, weight variation and folding endurance) using digital Vernier calliper and digital weighing balance. The physiochemical properties of the selected BFs films made of Carbopol (CP) and its combination with Pluronic F127 (PF127) were further characterized using XRD, DSC, FTIR, Transmission Electron Microscopy (TEM), EDS and Scanning Electron Microscopy (SEM). The permeation study of the selected BFs was investigated using Franz diffusion cell. The BFs composed of CP alone or its combination with PF127 demonstrated much better bio-adhesive properties than the films made of other polymers (like Hydroxyl propyl methyl cellulose, HPMC) alone or in combination with PF127. The developed liposome formulation showed high encapsulation 98.8 ± 0.01 % in CL to cholesterol mass ratio of 1:1 and total lipid to drug mass ratio of 2:1. The average particle size 104.82 ± 2.29 nm and Zeta potential -50.33 ± 0.95 mV of these liposomes were found to be attractive for targeted delivery to the HIV infected cells. The CP based BFs (without and with PF127) exhibited good film thickness 0.88 ± 0.10 and 0.76 ± 0.14 mm, with weight uniformity 68.22 ± 1.04 and 86.28 ± 2. 16 mg, satisfactory flexibility values 258 and 321, and slightly acidic pH 6.43 ± 0.76 and 6.32 ± 0.01. The swelling percentage was found to be 50 % for CP film alone and 78 % for CP film with PF127. The cumulative amount of drug that permeated through the buccal epithelium over 24 hours was about 66 % from CP film alone and 75 % from CP film with PF127. Since no evidence of the liposomal encapsulation of EFV have been reported to our knowledge, we find the insights from the present study valuable as a set of preliminary data to encourage further investigations of the encapsulation and delivery of EFV like antiretrovirals for enhanced solubility, site targeting and prolonged release using crude soybean lecithin and mucoadhesive polymers, which holds some added economical values as naturally occurring lipid and polymeric mixtures as a promising delivery systems for buccal delivery of ARVDs.
- Full Text:
- Date Issued: 2020
Functional conversion of commercial buildings in South Africa
- Authors: Kuliti, Gerald
- Date: 2020
- Subjects: Commercial buildings -- South Africa , Buildings -- Conservation and restoration Buildings -- Maintenance and repair
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10948/46075 , vital:39485
- Description: In the major cities of South Africa, there is a prevalence of originally built commercial buildings that are being converted into residential apartments or other industrial economic spaces. These secondary users refurbish the original structures to accommodate their new specialised requirements and this mostly happens after the buildings have been lying vacant for a long period of time. The study by Y.Long et al. (2011) narrates the changes in land use, identification of driving forces and the mapping of areas that are experiencing intense land use transformation. Properties that were developed for a focused particular use, were found to have been redeveloped within a few years for other functions. Subjects that were included in the sample were selected to meet specific criteria. The stakeholders had to meet the following criteria to be included in the sample: They were registered professionals in the built environment such as: Architecture, Engineering (civil, electrical, mechanical and structural), Quantity Surveying, Town Planning, Construction Management and Estate management The method that was used to collect data was self-completion questionnaire that was given out by electronic mail. After the data was collected, it was organised and analysed. For the analysis of closed-ended questions, an online programme called QuestionPro, was used, data was analysed by using descriptive statistics and frequency tables were drawn and from these, the data was presented in pie diagrams and bar graphs. While it is necessary to expand the horizons of the ever-growing global population, it is imperative that all stakeholders in the building industry work together to find sustainable solutions to residential and industrial development.
- Full Text:
- Date Issued: 2020
- Authors: Kuliti, Gerald
- Date: 2020
- Subjects: Commercial buildings -- South Africa , Buildings -- Conservation and restoration Buildings -- Maintenance and repair
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10948/46075 , vital:39485
- Description: In the major cities of South Africa, there is a prevalence of originally built commercial buildings that are being converted into residential apartments or other industrial economic spaces. These secondary users refurbish the original structures to accommodate their new specialised requirements and this mostly happens after the buildings have been lying vacant for a long period of time. The study by Y.Long et al. (2011) narrates the changes in land use, identification of driving forces and the mapping of areas that are experiencing intense land use transformation. Properties that were developed for a focused particular use, were found to have been redeveloped within a few years for other functions. Subjects that were included in the sample were selected to meet specific criteria. The stakeholders had to meet the following criteria to be included in the sample: They were registered professionals in the built environment such as: Architecture, Engineering (civil, electrical, mechanical and structural), Quantity Surveying, Town Planning, Construction Management and Estate management The method that was used to collect data was self-completion questionnaire that was given out by electronic mail. After the data was collected, it was organised and analysed. For the analysis of closed-ended questions, an online programme called QuestionPro, was used, data was analysed by using descriptive statistics and frequency tables were drawn and from these, the data was presented in pie diagrams and bar graphs. While it is necessary to expand the horizons of the ever-growing global population, it is imperative that all stakeholders in the building industry work together to find sustainable solutions to residential and industrial development.
- Full Text:
- Date Issued: 2020
Generation of a virtual library of terpenes using graph theory, and its application in exploration of the mechanisms of terpene biosynthesis
- Authors: Dendera, Washington
- Date: 2020
- Subjects: Terpenes , Plants -- Metabolism , Computational biology , Bioinformatics , Organic compounds -- Synthesis , Monoterpenes , Molecular biology -- Computer simulation
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/123453 , vital:35439
- Description: Terpenes form a large group of organic compounds which have proven to be of use to many living organisms being used by plants for metabolism (Pichersky and Gershenzon, 1934; McGarvey and Croteau, 1995; Gershenzon and Dudareva, 2007), defence or as a means to attract pollinators and also used by humans in medical, pharmaceutical and food industry (Bicas, Dionísio and Pastore, 2009; Marmulla and Harder, 2014; Kandi et al., 2015). Following on literature methods to generate chemical libraries using graph theoretic techniques, complete libraries of all possible terpene isomers have been constructed with the goal of construction of derivative libraries of possible carbocation intermediates which are important in the elucidation of mechanisms in the biosynthesis of terpenes. Virtual library generation of monoterpenes was first achieved by generating graphs of order 7, 8, 9 and 10 using the Nauty and Traces suite. These were screened and processed with a set of collated Python scripts written to recognize the graphs in text format and translate them to molecules, minimizing through Tinker whilst discarding graphs that violate chemistry laws. As a result of the computational time required only order 7 and order 10 graphs were processed. Out of the 873 graphs generated from order seven, 353 were converted to molecules and from the 11,7 million produced from order 10 half were processed resulting in the production of 442928 compounds (repeats included). For screening, 55 366 compounds were docked in the active site of limonene synthase; of these 2355 ligands had a good Vina docking score with a binding energy of between -7.0 and -7.4 kcal.mol-1. When these best docked molecules were overlaid in the active site a map of possible ligand positions within the active site of limonene synthase was traced out.
- Full Text:
- Date Issued: 2020
- Authors: Dendera, Washington
- Date: 2020
- Subjects: Terpenes , Plants -- Metabolism , Computational biology , Bioinformatics , Organic compounds -- Synthesis , Monoterpenes , Molecular biology -- Computer simulation
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/123453 , vital:35439
- Description: Terpenes form a large group of organic compounds which have proven to be of use to many living organisms being used by plants for metabolism (Pichersky and Gershenzon, 1934; McGarvey and Croteau, 1995; Gershenzon and Dudareva, 2007), defence or as a means to attract pollinators and also used by humans in medical, pharmaceutical and food industry (Bicas, Dionísio and Pastore, 2009; Marmulla and Harder, 2014; Kandi et al., 2015). Following on literature methods to generate chemical libraries using graph theoretic techniques, complete libraries of all possible terpene isomers have been constructed with the goal of construction of derivative libraries of possible carbocation intermediates which are important in the elucidation of mechanisms in the biosynthesis of terpenes. Virtual library generation of monoterpenes was first achieved by generating graphs of order 7, 8, 9 and 10 using the Nauty and Traces suite. These were screened and processed with a set of collated Python scripts written to recognize the graphs in text format and translate them to molecules, minimizing through Tinker whilst discarding graphs that violate chemistry laws. As a result of the computational time required only order 7 and order 10 graphs were processed. Out of the 873 graphs generated from order seven, 353 were converted to molecules and from the 11,7 million produced from order 10 half were processed resulting in the production of 442928 compounds (repeats included). For screening, 55 366 compounds were docked in the active site of limonene synthase; of these 2355 ligands had a good Vina docking score with a binding energy of between -7.0 and -7.4 kcal.mol-1. When these best docked molecules were overlaid in the active site a map of possible ligand positions within the active site of limonene synthase was traced out.
- Full Text:
- Date Issued: 2020
Gentrification in the Nelson Mandela Bay central business district
- Mageda, Nomthandazo Christellene
- Authors: Mageda, Nomthandazo Christellene
- Date: 2020
- Subjects: Gentrification , Urban renewal-- South Africa -- Nelson Mandela Metropolitan Municipality Neighborhoods -- South Africa -- Nelson Mandela Metropolitan Municipality
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10948/46119 , vital:39498
- Description: Gentrification is the process by which middle-class residents settle inner city neighborhood previously occupied by working-class. Gentrification has long been viewed as a consumption phenomenon, which is triggered by the urge of a certain fraction of a middle class – gentrifies – to create and maintain distinction. The research is designed as a socio-economic account to discuss the development in the NMB CBD area. In my research I examine the multiscalar forces affecting neighborhood change. This study will also examine the elements affecting and also the benefits of the gentrification process using quantitative methods. The CBD of the NMB represents certain universalities of gentrification in an old city. Today the city is no longer the regular of development but instead its progenitor. Adopting the language of “urbanization” and “cities of the future” will be considered in gearing the economic development arm. It does so by an extensive mapping of gentrification processes in Port Elizabeth. It also contains a smaller study on gentrification in Cape Town and also on an international level. The outcomes provide telling insights into gentrification as a generic process affecting not just low income areas (classical gentrification) and high income areas (super-gentrification), but also a wide-ranging spectrum of medium-income areas (ordinary gentrification). This study examines the interdependent variables of gentrification, going beyond a profitable analysis and incorporating the voice of all the actors involved in the process of gentrification, using quantitative analysis. By taking into account all perspectives, this study permits a holistic understanding of the social costs and benefits associated with gentrification. As multifaceted as gentrification is, it is becoming more pervasive in cities globally, a holistic tally of the social costs and benefits of revitalization projects becoming imperative. The study concludes with discussions of the contributions, limitations, recommendations for the NMB CBD and other cities that are considering revitalizing their urban cores and implications for future research.
- Full Text:
- Date Issued: 2020
- Authors: Mageda, Nomthandazo Christellene
- Date: 2020
- Subjects: Gentrification , Urban renewal-- South Africa -- Nelson Mandela Metropolitan Municipality Neighborhoods -- South Africa -- Nelson Mandela Metropolitan Municipality
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10948/46119 , vital:39498
- Description: Gentrification is the process by which middle-class residents settle inner city neighborhood previously occupied by working-class. Gentrification has long been viewed as a consumption phenomenon, which is triggered by the urge of a certain fraction of a middle class – gentrifies – to create and maintain distinction. The research is designed as a socio-economic account to discuss the development in the NMB CBD area. In my research I examine the multiscalar forces affecting neighborhood change. This study will also examine the elements affecting and also the benefits of the gentrification process using quantitative methods. The CBD of the NMB represents certain universalities of gentrification in an old city. Today the city is no longer the regular of development but instead its progenitor. Adopting the language of “urbanization” and “cities of the future” will be considered in gearing the economic development arm. It does so by an extensive mapping of gentrification processes in Port Elizabeth. It also contains a smaller study on gentrification in Cape Town and also on an international level. The outcomes provide telling insights into gentrification as a generic process affecting not just low income areas (classical gentrification) and high income areas (super-gentrification), but also a wide-ranging spectrum of medium-income areas (ordinary gentrification). This study examines the interdependent variables of gentrification, going beyond a profitable analysis and incorporating the voice of all the actors involved in the process of gentrification, using quantitative analysis. By taking into account all perspectives, this study permits a holistic understanding of the social costs and benefits associated with gentrification. As multifaceted as gentrification is, it is becoming more pervasive in cities globally, a holistic tally of the social costs and benefits of revitalization projects becoming imperative. The study concludes with discussions of the contributions, limitations, recommendations for the NMB CBD and other cities that are considering revitalizing their urban cores and implications for future research.
- Full Text:
- Date Issued: 2020