Consumer perceptions and microbial quality of meat sold in the informal markets of Nkonkobe and Buffalo City municipalities in the Eastern province, South Africa
- Authors: Mazizi, Bulelani Elvis
- Date: 2016
- Subjects: Meat -- Quality -- South Africa --Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/15365 , vital:40401
- Description: The main objective of the study was to determine consumer perceptions and microbial quality of meat sold in the informal markets found in Nkonkobe and Buffalo City Municipalities in the Eastern Cape Province, South Africa. To determine consumer perceptions of the quality of the meat and meat products sold in the informal markets in Nkonkobe and Buffalo City Municipalities, a survey was conducted. A total of 160 consumers from two municipalities in the Eastern Cape (EC) Province of South Africa was randomly sampled and interviewed. A questionnaire was used to gather information about consumer perceptions of meat and meat products sold in the informal markets regarding their knowledge about safety, food-borne diseases, hygiene, and the quality of the meat among others. The study showed that Alice Town has a larger (66.25percent) proportion of consumers with knowledge about foodborne diseases while King Williams’s Town had a larger proportion (48.75percent) of respondents who had no knowledge about Food-borne diseases. However, a large proportion of consumers in King Williams’s Town considered vendor (82.5percent) and environmental hygiene (93.75percent) than in Alice Town. Consumers (83.75percent) in King Williams’s Town viewed the meat from street vendors to be of low quality while a small proportion (18.75percent) of consumers in Alice Town viewed it as the meat of good quality. Alice had a large proportion (61.25percent) of consumers, which had knowledge of the meat safety law than King Williams’s Town. More consumers from King Williams’s Town (31.25percent) agreed that government plays a role while Alice had a small proportion (26.25percent) which disagreed. The results also showed that consumer perceptions about the safety of meat and meat products are focused more on hygiene, neglecting other important aspects such as vending infrastructure. However, most consumers do not have enough knowledge concerning meat safety. The second study determined the microbial quality of meat sold by street vendors based in Alice and Kind Williams Town in the Eastern Cape Province South Africa. A total of 48 fresh and uncooked meat samples (beef, pork, and mutton), 48 surface contact plates and 40 water samples was collected from 4 street vendors from each town for microbial analysis (n=48). After collection, all samples were subjected to Aerobic plate count (APC), Salmonella spp, Staphylococcus aureus (Staph) and Escherichia coli (E. coli). The results revealed that Salmonella spp tested negative across all the meat samples used in the study. However, no significant differences were found in the microbial quality of meat sold by street vendors in Alice and King Williams town. Furthermore, there were no significant differences (P>0.05) between the APC of raw beef (4.8 Log CFU/g), mutton (3.7 Log CFU/g) and pork (2.8 Log CFU/g) and also the cooked beef (1.5 Log CFU/g), mutton (1.3 Log CFU/g) and pork (1.9 Log CFU/g) samples. A similar trend was observed in the values of Staphylococcus aureus (Staph) and Escherichia coli (E. coli) counts in raw and cooked meat samples. However, a positive correlation between Aerobic plate counts (APC) and Staphylococcus aureus (Staph) (P<0.001) and Escherichia coli (E. coli) (P<0.01) was found in the study. It can, therefore, be concluded that there were no differences in the microbial counts of raw and cooked meat sold in the informal markets of Nkonkobe and Buffalo City Municipalities in the Eastern Cape Province, South Africa.
- Full Text:
- Authors: Mazizi, Bulelani Elvis
- Date: 2016
- Subjects: Meat -- Quality -- South Africa --Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/15365 , vital:40401
- Description: The main objective of the study was to determine consumer perceptions and microbial quality of meat sold in the informal markets found in Nkonkobe and Buffalo City Municipalities in the Eastern Cape Province, South Africa. To determine consumer perceptions of the quality of the meat and meat products sold in the informal markets in Nkonkobe and Buffalo City Municipalities, a survey was conducted. A total of 160 consumers from two municipalities in the Eastern Cape (EC) Province of South Africa was randomly sampled and interviewed. A questionnaire was used to gather information about consumer perceptions of meat and meat products sold in the informal markets regarding their knowledge about safety, food-borne diseases, hygiene, and the quality of the meat among others. The study showed that Alice Town has a larger (66.25percent) proportion of consumers with knowledge about foodborne diseases while King Williams’s Town had a larger proportion (48.75percent) of respondents who had no knowledge about Food-borne diseases. However, a large proportion of consumers in King Williams’s Town considered vendor (82.5percent) and environmental hygiene (93.75percent) than in Alice Town. Consumers (83.75percent) in King Williams’s Town viewed the meat from street vendors to be of low quality while a small proportion (18.75percent) of consumers in Alice Town viewed it as the meat of good quality. Alice had a large proportion (61.25percent) of consumers, which had knowledge of the meat safety law than King Williams’s Town. More consumers from King Williams’s Town (31.25percent) agreed that government plays a role while Alice had a small proportion (26.25percent) which disagreed. The results also showed that consumer perceptions about the safety of meat and meat products are focused more on hygiene, neglecting other important aspects such as vending infrastructure. However, most consumers do not have enough knowledge concerning meat safety. The second study determined the microbial quality of meat sold by street vendors based in Alice and Kind Williams Town in the Eastern Cape Province South Africa. A total of 48 fresh and uncooked meat samples (beef, pork, and mutton), 48 surface contact plates and 40 water samples was collected from 4 street vendors from each town for microbial analysis (n=48). After collection, all samples were subjected to Aerobic plate count (APC), Salmonella spp, Staphylococcus aureus (Staph) and Escherichia coli (E. coli). The results revealed that Salmonella spp tested negative across all the meat samples used in the study. However, no significant differences were found in the microbial quality of meat sold by street vendors in Alice and King Williams town. Furthermore, there were no significant differences (P>0.05) between the APC of raw beef (4.8 Log CFU/g), mutton (3.7 Log CFU/g) and pork (2.8 Log CFU/g) and also the cooked beef (1.5 Log CFU/g), mutton (1.3 Log CFU/g) and pork (1.9 Log CFU/g) samples. A similar trend was observed in the values of Staphylococcus aureus (Staph) and Escherichia coli (E. coli) counts in raw and cooked meat samples. However, a positive correlation between Aerobic plate counts (APC) and Staphylococcus aureus (Staph) (P<0.001) and Escherichia coli (E. coli) (P<0.01) was found in the study. It can, therefore, be concluded that there were no differences in the microbial counts of raw and cooked meat sold in the informal markets of Nkonkobe and Buffalo City Municipalities in the Eastern Cape Province, South Africa.
- Full Text:
Creation and detection of Vector Bessel Beams
- Omoefe, Idisi David, Forbes, Andrew
- Authors: Omoefe, Idisi David , Forbes, Andrew
- Date: 2016
- Subjects: Vector analysis Quantum theory
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/1785 , vital:27558
- Description: Bessel beams are optical fields which falls into the category of non-diffracting beams. Vector Bessel beams are vector beams possessing cylindrical symmetry. Cylindrically symmetric beams tend to have a tight focal point during propagation. The tight focal beam nature of vector Bessel beams makes them a good potential in various facets of science such as biological optical trapping, wireless communications, remote sensing, microscopy etc. In this research work, vector Bessel beams were generated using the phase of an Axicon that was encoded into a spatial light modulator. Firstly, scalar Bessel beams which possess linear polarization were generated and converted to circularly polarized vector beams by the use of a q-plate. The orbital angular momentum (OAM) modes that are embedded in the vortex beams were detected using modal decomposition technique. This was implemented for both the scalar and vector case using a quarter wave plate. The measure of the degree of non-separability of the vector Bessel beams using tomographic quantum tools was also implemented where the density matrix was reconstructed. The concurrence and fidelity which explore the measure of vectorness of both scalar and vector Bessel beams were calculated from the density matrix. The obtained results show that the spatial modes and polarization are coupled in the vector case as expected.
- Full Text:
- Authors: Omoefe, Idisi David , Forbes, Andrew
- Date: 2016
- Subjects: Vector analysis Quantum theory
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/1785 , vital:27558
- Description: Bessel beams are optical fields which falls into the category of non-diffracting beams. Vector Bessel beams are vector beams possessing cylindrical symmetry. Cylindrically symmetric beams tend to have a tight focal point during propagation. The tight focal beam nature of vector Bessel beams makes them a good potential in various facets of science such as biological optical trapping, wireless communications, remote sensing, microscopy etc. In this research work, vector Bessel beams were generated using the phase of an Axicon that was encoded into a spatial light modulator. Firstly, scalar Bessel beams which possess linear polarization were generated and converted to circularly polarized vector beams by the use of a q-plate. The orbital angular momentum (OAM) modes that are embedded in the vortex beams were detected using modal decomposition technique. This was implemented for both the scalar and vector case using a quarter wave plate. The measure of the degree of non-separability of the vector Bessel beams using tomographic quantum tools was also implemented where the density matrix was reconstructed. The concurrence and fidelity which explore the measure of vectorness of both scalar and vector Bessel beams were calculated from the density matrix. The obtained results show that the spatial modes and polarization are coupled in the vector case as expected.
- Full Text:
Crop rotation and crop residue management effects under no till on the soil quality of two ecotopes in the Eastern Cape, South Africa
- Authors: Isaac, Gura
- Date: 2016
- Subjects: Crop rotation Crops and soils Soil fertility
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/2934 , vital:28144
- Description: The degradation of soil quality due to undesirable farming practices has reached alarming scales in the Eastern Cape and this has had negative repercussions on soil productivity and the environment in general. There is growing evidence that conservation agriculture (CA) practices involving minimal mechanical disturbance, maintaining permanent surface cover and embracing diverse crop rotations increase soil organic carbon (SOC) and therefore has potential to mitigate soil quality deterioration. A study was carried out at two sites located in two ecotopes to investigate the effects of crop residue retention and crop rotations in a no till system on overall soil quality using the Soil Management Assessment Framework (SMAF) as the soil quality assessment tool. The CA study trials were laid out in 2012 at two different locations, one at the Phandulwazi Agricultural High school within the Phandulwazi Jozini ecotope and the other one at University of Fort Hare Research Farm within the Alice Jozini ecotope. The experiment was laid out as a split-split plot arrangement in a randomized complete block design with three replicates. Tillage treatments were applied on the main plots while crop rotation treatments were applied as subplots. Crop residue retention treatments were applied as sub-sub plots. The rotational treatments were maize-fallow-maize (MFM), maize-fallow-soybean (MFS), maize-wheat-maize (MWM) and maize-wheat-soybean (MWS). The initial assessment of the overall soil quality of the two ecotopes using the SMAF soil quality index (SQI) revealed that the soils at the Alice site were functioning at 80% while the soils at the Phandulwazi site were functioning at 79 percent of their optimum capacity. The slight difference in the soil quality of the two ecotopes could be attributed to their different soil organic C contents where the Alice Jozini ecotope had significantly higher soil organic C contents than the Phandulwazi Jozini ecotope. After 3 years of continuous treatment application, crop residue retention significantly improved most of the measured soil quality parameters. Generally across the sites, more soil organic C, microbial biomass C (MBC), ß-glucosidase (BG) activity, mineral N, extractable P and K, Cu, Zn, Mn, Fe, and macro-aggregates were recorded in treatments where crop residues were retained. Crop rotations alone did not have a significant impact on most of the measured soil quality indicators. The crop rotations influenced significantly the availability of mineral N across the two sites, highlighting the importance of using a legume in rotations on available N for the subsequent crops. Most of the measured soil attributes were not significantly influenced after 3 years of continuously applying combined treatment of CA components. Mineral N (NO3 + NH4), K, Zn and Fe were significantly impacted on by the interactions of CA components at the Phandulwazi site, while N, Cu, Zn and Mn were significantly increased at the Alice site. Low response of SOC to combined CA treatments in the short-term prompted the need to examine treatment effects on individual soil carbon fractions. The interaction of crop rotation and residue management techniques were significant on the fine particulate organic matter – C fractions and microbially respired C. These soil C fractions were more sensitive to short-term treatments of combined CA components than SOC and MBC, therefore they can be used as short-term indicators of CA effects on SOM. Soil organic carbon, MBC, extractable P and K, soil pH, EC, b, AGS (aggregate stability) and BG activity were measured and the Soil Management Assessment Framework (SMAF) used to calculate soil quality index (SQI) values for each treatment. The combination of the crop rotations with crop residue retention showed the potential to significantly improve SQI values in the long term. The highest soil quality improvement at both sites was achieved by the maize-wheat-soybean (MWS) rotation with crop residue retention.
- Full Text:
- Authors: Isaac, Gura
- Date: 2016
- Subjects: Crop rotation Crops and soils Soil fertility
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/2934 , vital:28144
- Description: The degradation of soil quality due to undesirable farming practices has reached alarming scales in the Eastern Cape and this has had negative repercussions on soil productivity and the environment in general. There is growing evidence that conservation agriculture (CA) practices involving minimal mechanical disturbance, maintaining permanent surface cover and embracing diverse crop rotations increase soil organic carbon (SOC) and therefore has potential to mitigate soil quality deterioration. A study was carried out at two sites located in two ecotopes to investigate the effects of crop residue retention and crop rotations in a no till system on overall soil quality using the Soil Management Assessment Framework (SMAF) as the soil quality assessment tool. The CA study trials were laid out in 2012 at two different locations, one at the Phandulwazi Agricultural High school within the Phandulwazi Jozini ecotope and the other one at University of Fort Hare Research Farm within the Alice Jozini ecotope. The experiment was laid out as a split-split plot arrangement in a randomized complete block design with three replicates. Tillage treatments were applied on the main plots while crop rotation treatments were applied as subplots. Crop residue retention treatments were applied as sub-sub plots. The rotational treatments were maize-fallow-maize (MFM), maize-fallow-soybean (MFS), maize-wheat-maize (MWM) and maize-wheat-soybean (MWS). The initial assessment of the overall soil quality of the two ecotopes using the SMAF soil quality index (SQI) revealed that the soils at the Alice site were functioning at 80% while the soils at the Phandulwazi site were functioning at 79 percent of their optimum capacity. The slight difference in the soil quality of the two ecotopes could be attributed to their different soil organic C contents where the Alice Jozini ecotope had significantly higher soil organic C contents than the Phandulwazi Jozini ecotope. After 3 years of continuous treatment application, crop residue retention significantly improved most of the measured soil quality parameters. Generally across the sites, more soil organic C, microbial biomass C (MBC), ß-glucosidase (BG) activity, mineral N, extractable P and K, Cu, Zn, Mn, Fe, and macro-aggregates were recorded in treatments where crop residues were retained. Crop rotations alone did not have a significant impact on most of the measured soil quality indicators. The crop rotations influenced significantly the availability of mineral N across the two sites, highlighting the importance of using a legume in rotations on available N for the subsequent crops. Most of the measured soil attributes were not significantly influenced after 3 years of continuously applying combined treatment of CA components. Mineral N (NO3 + NH4), K, Zn and Fe were significantly impacted on by the interactions of CA components at the Phandulwazi site, while N, Cu, Zn and Mn were significantly increased at the Alice site. Low response of SOC to combined CA treatments in the short-term prompted the need to examine treatment effects on individual soil carbon fractions. The interaction of crop rotation and residue management techniques were significant on the fine particulate organic matter – C fractions and microbially respired C. These soil C fractions were more sensitive to short-term treatments of combined CA components than SOC and MBC, therefore they can be used as short-term indicators of CA effects on SOM. Soil organic carbon, MBC, extractable P and K, soil pH, EC, b, AGS (aggregate stability) and BG activity were measured and the Soil Management Assessment Framework (SMAF) used to calculate soil quality index (SQI) values for each treatment. The combination of the crop rotations with crop residue retention showed the potential to significantly improve SQI values in the long term. The highest soil quality improvement at both sites was achieved by the maize-wheat-soybean (MWS) rotation with crop residue retention.
- Full Text:
Design and implementation of a multi-agent opportunistic grid computing platform
- Authors: Muranganwa, Raymond
- Date: 2016
- Subjects: Computational grids (Computer systems)
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/2722 , vital:28064
- Description: Opportunistic Grid Computing involves joining idle computing resources in enterprises into a converged high performance commodity infrastructure. The research described in this dissertation investigates the viability of public resource computing in offering a plethora of possibilities through seamless access to shared compute and storage resources. The research proposes and conceptualizes the Multi-Agent Opportunistic Grid (MAOG) solution in an Information and Communication Technologies for Development (ICT4D) initiative to address some limitations prevalent in traditional distributed system implementations. Proof-of-concept software components based on JADE (Java Agent Development Framework) validated Multi-Agent Systems (MAS) as an important tool for provisioning of Opportunistic Grid Computing platforms. Exploration of agent technologies within the research context identified two key components which improve access to extended computer capabilities. The first component is a Mobile Agent (MA) compute component in which a group of agents interact to pool shared processor cycles. The compute component integrates dynamic resource identification and allocation strategies by incorporating the Contract Net Protocol (CNP) and rule based reasoning concepts. The second service is a MAS based storage component realized through disk mirroring and Google file-system’s chunking with atomic append storage techniques. This research provides a candidate Opportunistic Grid Computing platform design and implementation through the use of MAS. Experiments conducted validated the design and implementation of the compute and storage services. From results, support for processing user applications; resource identification and allocation; and rule based reasoning validated the MA compute component. A MAS based file-system that implements chunking optimizations was considered to be optimum based on evaluations. The findings from the undertaken experiments also validated the functional adequacy of the implementation, and show the suitability of MAS for provisioning of robust, autonomous, and intelligent platforms. The context of this research, ICT4D, provides a solution to optimizing and increasing the utilization of computing resources that are usually idle in these contexts.
- Full Text:
- Authors: Muranganwa, Raymond
- Date: 2016
- Subjects: Computational grids (Computer systems)
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/2722 , vital:28064
- Description: Opportunistic Grid Computing involves joining idle computing resources in enterprises into a converged high performance commodity infrastructure. The research described in this dissertation investigates the viability of public resource computing in offering a plethora of possibilities through seamless access to shared compute and storage resources. The research proposes and conceptualizes the Multi-Agent Opportunistic Grid (MAOG) solution in an Information and Communication Technologies for Development (ICT4D) initiative to address some limitations prevalent in traditional distributed system implementations. Proof-of-concept software components based on JADE (Java Agent Development Framework) validated Multi-Agent Systems (MAS) as an important tool for provisioning of Opportunistic Grid Computing platforms. Exploration of agent technologies within the research context identified two key components which improve access to extended computer capabilities. The first component is a Mobile Agent (MA) compute component in which a group of agents interact to pool shared processor cycles. The compute component integrates dynamic resource identification and allocation strategies by incorporating the Contract Net Protocol (CNP) and rule based reasoning concepts. The second service is a MAS based storage component realized through disk mirroring and Google file-system’s chunking with atomic append storage techniques. This research provides a candidate Opportunistic Grid Computing platform design and implementation through the use of MAS. Experiments conducted validated the design and implementation of the compute and storage services. From results, support for processing user applications; resource identification and allocation; and rule based reasoning validated the MA compute component. A MAS based file-system that implements chunking optimizations was considered to be optimum based on evaluations. The findings from the undertaken experiments also validated the functional adequacy of the implementation, and show the suitability of MAS for provisioning of robust, autonomous, and intelligent platforms. The context of this research, ICT4D, provides a solution to optimizing and increasing the utilization of computing resources that are usually idle in these contexts.
- Full Text:
Determinants of participation in beekeeping and its contribution to rural household income: the case of O. R. Tambo Municipality, Eastern Cape Province, South Africa
- Authors: Ncetani, Nelisiwe
- Date: 2016
- Subjects: Beekeepers -- South Africa -- Eastern Cape Sustainable living -- South Africa -- Eastern Cape Home economics, Rural -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/1486 , vital:27299
- Description: This dissertation explores determinants and perceptions to participation in beekeeping by households’ as well as its contribution to household income. This is in a context that smallholder farmers’ participation is reported to be very low, despite several claimed benefits of beekeeping. Moreover, despite receiving substantial endorsements as a resilient climate-smart rural livelihood, off-farm, forest, non-timber diversification strategy; the uptake of beekeeping by smallholder farmers in the Eastern Cape Province of South Africa still remains very low. Against this background, this study used cross-sectional survey data to gauge smallholder farmers’ perceptions and factors that influence their participation in beekeeping using a binary regression model; further, it measured the contribution of beekeeping to rural household income using a linear regression model. Descriptive statistics revealed that majority of the people from the study area have positive perceptions regarding beekeeping. Regression estimates further indicate that beekeeping is mostly conditioned by shared perceptions, institutional and socio-economic factors that are worth targeting to promote the uptake of beekeeping as a livelihood. Public policies that influence the institutional framework (extension, credit and market) in favour of beekeeping are more likely to promote participation in beekeeping activities. Also, more research on the documentation and benefits of beekeeping supported by investments targeting educational campaigns towards promoting positive attitudes and dispelling fears and myths surrounding beekeeping as an enterprise, will also promote the participation of smallholder farmers in beekeeping. Regression estimates for determinants of household income revealed a significant positive contribution of beekeeping to household income, education, gender and age. Promotion of beekeeping may therefore address household income that is worth targeting to address rural poverty. The study therefore concludes that to fully unlock the income potential of beekeeping for smallholder farmers, development agencies need to address several negative perceptions and institutional factors that affect beekeeping participation, while more research is required to quantify the claimed benefits of beekeeping, given their positive potential to promote farmers’ interests in beekeeping.
- Full Text:
- Authors: Ncetani, Nelisiwe
- Date: 2016
- Subjects: Beekeepers -- South Africa -- Eastern Cape Sustainable living -- South Africa -- Eastern Cape Home economics, Rural -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/1486 , vital:27299
- Description: This dissertation explores determinants and perceptions to participation in beekeeping by households’ as well as its contribution to household income. This is in a context that smallholder farmers’ participation is reported to be very low, despite several claimed benefits of beekeeping. Moreover, despite receiving substantial endorsements as a resilient climate-smart rural livelihood, off-farm, forest, non-timber diversification strategy; the uptake of beekeeping by smallholder farmers in the Eastern Cape Province of South Africa still remains very low. Against this background, this study used cross-sectional survey data to gauge smallholder farmers’ perceptions and factors that influence their participation in beekeeping using a binary regression model; further, it measured the contribution of beekeeping to rural household income using a linear regression model. Descriptive statistics revealed that majority of the people from the study area have positive perceptions regarding beekeeping. Regression estimates further indicate that beekeeping is mostly conditioned by shared perceptions, institutional and socio-economic factors that are worth targeting to promote the uptake of beekeeping as a livelihood. Public policies that influence the institutional framework (extension, credit and market) in favour of beekeeping are more likely to promote participation in beekeeping activities. Also, more research on the documentation and benefits of beekeeping supported by investments targeting educational campaigns towards promoting positive attitudes and dispelling fears and myths surrounding beekeeping as an enterprise, will also promote the participation of smallholder farmers in beekeeping. Regression estimates for determinants of household income revealed a significant positive contribution of beekeeping to household income, education, gender and age. Promotion of beekeeping may therefore address household income that is worth targeting to address rural poverty. The study therefore concludes that to fully unlock the income potential of beekeeping for smallholder farmers, development agencies need to address several negative perceptions and institutional factors that affect beekeeping participation, while more research is required to quantify the claimed benefits of beekeeping, given their positive potential to promote farmers’ interests in beekeeping.
- Full Text:
Development of a stemmer for the isiXhosa language
- Authors: Nogwina, Mnoneleli
- Date: 2016
- Subjects: Computational linguistics Language and languages Xhosa language
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/2611 , vital:27938
- Description: IsiXhosa language is one of the eleven official languages and the second most widely spoken language in South Africa. However, in terms of computational linguistics, the language did not get attention and natural language related work is almost non-existent. Document retrieval using unstructured queries requires some kind of language processing, and an efficient retrieval of documents can be achieved if we use a technique called stemming. The area that involves document storage and retrieval is called Information Retrieval (IR). Basically, IR systems make use of a Stemmer to index document representations and also terms in users’ queries to retrieve matching documents. In this dissertation, we present the developed Stemmer that can be used in both conditions. The Stemmer is used in IR systems, like Google to retrieve documents written in isiXhosa. In the Eastern Cape Province of South Africa many public schools take isiXhosa as a subject and also a number of Universities in South Africa teach isiXhosa. Therefore, for a language important such as this, it is important to make valuable information that is available online accessible to users through the use of IR systems. In our efforts to develop a Stemmer for the isiXhosa language, an investigation on how others have developed Stemmers for other languages was carried out. From the investigation we came to realize that the Porter stemming algorithm in particular was the main algorithm that many of other Stemmers make use of as a reference. We found that Porter’s algorithm could not be used in its totality in the development of the isiXhosa Stemmer because of the morphological complexity of the language. We developed an affix removal that is embedded with rules that determine which order should be followed in stripping the affixes. The rule is that, the word under consideration is checked against the exceptions, if it’s not in the exceptions list then the stripping continue in the following order; Prefix removal, Suffix removal and finally save the result as stem. The Stemmer was successfully developed and was tested and evaluated in a sample data that was randomly collected from the isiXhosa text books and isiXhosa dictionary. From the results obtained we concluded that the Stemmer can be used in IR systems as it showed 91 percent accuracy. The errors were 9 percent and therefore these results are within the accepted range and therefore the Stemmer can be used to help in retrieval of isiXhosa documents. This is only a noun Stemmer and in the future it can be extended to also stem verbs as well. The Stemmer can also be used in the development of spell-checkers of isiXhosa.
- Full Text:
- Authors: Nogwina, Mnoneleli
- Date: 2016
- Subjects: Computational linguistics Language and languages Xhosa language
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/2611 , vital:27938
- Description: IsiXhosa language is one of the eleven official languages and the second most widely spoken language in South Africa. However, in terms of computational linguistics, the language did not get attention and natural language related work is almost non-existent. Document retrieval using unstructured queries requires some kind of language processing, and an efficient retrieval of documents can be achieved if we use a technique called stemming. The area that involves document storage and retrieval is called Information Retrieval (IR). Basically, IR systems make use of a Stemmer to index document representations and also terms in users’ queries to retrieve matching documents. In this dissertation, we present the developed Stemmer that can be used in both conditions. The Stemmer is used in IR systems, like Google to retrieve documents written in isiXhosa. In the Eastern Cape Province of South Africa many public schools take isiXhosa as a subject and also a number of Universities in South Africa teach isiXhosa. Therefore, for a language important such as this, it is important to make valuable information that is available online accessible to users through the use of IR systems. In our efforts to develop a Stemmer for the isiXhosa language, an investigation on how others have developed Stemmers for other languages was carried out. From the investigation we came to realize that the Porter stemming algorithm in particular was the main algorithm that many of other Stemmers make use of as a reference. We found that Porter’s algorithm could not be used in its totality in the development of the isiXhosa Stemmer because of the morphological complexity of the language. We developed an affix removal that is embedded with rules that determine which order should be followed in stripping the affixes. The rule is that, the word under consideration is checked against the exceptions, if it’s not in the exceptions list then the stripping continue in the following order; Prefix removal, Suffix removal and finally save the result as stem. The Stemmer was successfully developed and was tested and evaluated in a sample data that was randomly collected from the isiXhosa text books and isiXhosa dictionary. From the results obtained we concluded that the Stemmer can be used in IR systems as it showed 91 percent accuracy. The errors were 9 percent and therefore these results are within the accepted range and therefore the Stemmer can be used to help in retrieval of isiXhosa documents. This is only a noun Stemmer and in the future it can be extended to also stem verbs as well. The Stemmer can also be used in the development of spell-checkers of isiXhosa.
- Full Text:
Development of part-of-speech tagger for Xhosa
- Authors: Delman, Xolani
- Date: 2016
- Subjects: Computational linguistics -- Methodology Natural language processing (Computer science) Linguistic models
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/11872 , vital:39114
- Description: Part-of-Speech (POS) tagging is a process of assigning an appropriate part of speech or lexical category to each word in a given sentence of a particular natural language. Natural languages are languages that human beings use to communicate with one another be it Xhosa, Zulu, English etc. POS tagging plays a huge and important role in natural language processing applications. The main applications of POS tagging include machine translation, parsing, text chunking, spell checkiXhosa (sometimes referred to as isiXhosa) is one of the eleven official languages of South Africa and is spoken by over 8 million South Africans. The language is mainly spoken in the Eastern Cape and Western Cape provinces of the country. It is the second most widely spoken native language in South Africa after Zulu (sometimes called isiZulu). Although the number of speakers might seem to be high, Xhosa is considerably under-resourced. There are very few publications in Xhosa, very few books have been published in the language and also the domains that use the language as a medium of instruction are very limited. However, the language is finding momentum nowadays. An Oxford approved Xhosa dictionary has been developed recently, and Xhosa newspapers that did not exist in the recent past are now published. Text from previously mentioned sources can then be combined to formulate a larger text that can be used to train the tagger. This work aims to develop an effective POS tagger for Xhosa. g and grammar. This thesis presents/describes the work that needed to be done to produce an automatic POS tagger for Xhosa. A tagset consisting of 36 POS tags/labels for the language were used for this purpose. These are listed. A total of 5000 words were manually tagged/labelled for the purpose of training the tagger. Another 3000 words were used for testing the tagger and these were disjoint from the manually tagged training data. The open source Stanford CoreNLP toolkit was used to create the tagger. The toolkit implements a Maximum Entropy machine learning model which was applied in the development of the tagger presented in this thesis. The thesis describes the implementation and testing processes of the model in detail. The results show that the development of the Xhosa POS tagging model was successful. This model managed to obtain a tagging accuracy of 87.71 percent.
- Full Text:
- Authors: Delman, Xolani
- Date: 2016
- Subjects: Computational linguistics -- Methodology Natural language processing (Computer science) Linguistic models
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/11872 , vital:39114
- Description: Part-of-Speech (POS) tagging is a process of assigning an appropriate part of speech or lexical category to each word in a given sentence of a particular natural language. Natural languages are languages that human beings use to communicate with one another be it Xhosa, Zulu, English etc. POS tagging plays a huge and important role in natural language processing applications. The main applications of POS tagging include machine translation, parsing, text chunking, spell checkiXhosa (sometimes referred to as isiXhosa) is one of the eleven official languages of South Africa and is spoken by over 8 million South Africans. The language is mainly spoken in the Eastern Cape and Western Cape provinces of the country. It is the second most widely spoken native language in South Africa after Zulu (sometimes called isiZulu). Although the number of speakers might seem to be high, Xhosa is considerably under-resourced. There are very few publications in Xhosa, very few books have been published in the language and also the domains that use the language as a medium of instruction are very limited. However, the language is finding momentum nowadays. An Oxford approved Xhosa dictionary has been developed recently, and Xhosa newspapers that did not exist in the recent past are now published. Text from previously mentioned sources can then be combined to formulate a larger text that can be used to train the tagger. This work aims to develop an effective POS tagger for Xhosa. g and grammar. This thesis presents/describes the work that needed to be done to produce an automatic POS tagger for Xhosa. A tagset consisting of 36 POS tags/labels for the language were used for this purpose. These are listed. A total of 5000 words were manually tagged/labelled for the purpose of training the tagger. Another 3000 words were used for testing the tagger and these were disjoint from the manually tagged training data. The open source Stanford CoreNLP toolkit was used to create the tagger. The toolkit implements a Maximum Entropy machine learning model which was applied in the development of the tagger presented in this thesis. The thesis describes the implementation and testing processes of the model in detail. The results show that the development of the Xhosa POS tagging model was successful. This model managed to obtain a tagging accuracy of 87.71 percent.
- Full Text:
Dynamic service orchestration in heterogeneous internet of things environments
- Authors: Chindenga, Edmore
- Date: 2016
- Subjects: Internet of things
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/8001 , vital:31457
- Description: Internet of Things (IoT) presents a dynamic global revolution in the Internet where physical and virtual “things” will communicate and share information. As the number of devices increases, there is a need for a plug-and–interoperate approach of deploying “things” to the existing network with less or no human need for configuration. The plug-and-interoperate approach allows heterogeneous “things” to seamlessly interoperate, interact and exchange information and subsequently share services. Services are represented as functionalities that are offered by the “things”. Service orchestration provides an approach to integration and interoperability that decouples applications from each other, enhancing capabilities to centrally manage and monitor components. This work investigated requirements for semantic interoperability and exposed current challenges in IoT interoperability as a means of facilitating services orchestration in IoT. The research proposes a platform that allows heterogeneous devices to collaborate thereby enabling dynamic service orchestration. The platform provides a common framework for representing semantics allowing for a consistent information exchange format. The information is stored and presented in an ontology thereby preserving semantics and making the information comprehensible to machines allowing for automated addressing, tracking and discovery as well as information representation, storage, and exchange. Process mining techniques were used to discover service orchestrations. Process mining techniques enabled the analysis of runtime behavior of service orchestrations and the semantic breakdown of the service request and creation in real time. This enabled the research to draw observations that led to conclusions presented in this work. The research noted that the use of semantic technologies facilitates interoperability in heterogeneous devices and can be implemented as a means to bypass challenges presented by differences in IoT “things”.
- Full Text:
- Authors: Chindenga, Edmore
- Date: 2016
- Subjects: Internet of things
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/8001 , vital:31457
- Description: Internet of Things (IoT) presents a dynamic global revolution in the Internet where physical and virtual “things” will communicate and share information. As the number of devices increases, there is a need for a plug-and–interoperate approach of deploying “things” to the existing network with less or no human need for configuration. The plug-and-interoperate approach allows heterogeneous “things” to seamlessly interoperate, interact and exchange information and subsequently share services. Services are represented as functionalities that are offered by the “things”. Service orchestration provides an approach to integration and interoperability that decouples applications from each other, enhancing capabilities to centrally manage and monitor components. This work investigated requirements for semantic interoperability and exposed current challenges in IoT interoperability as a means of facilitating services orchestration in IoT. The research proposes a platform that allows heterogeneous devices to collaborate thereby enabling dynamic service orchestration. The platform provides a common framework for representing semantics allowing for a consistent information exchange format. The information is stored and presented in an ontology thereby preserving semantics and making the information comprehensible to machines allowing for automated addressing, tracking and discovery as well as information representation, storage, and exchange. Process mining techniques were used to discover service orchestrations. Process mining techniques enabled the analysis of runtime behavior of service orchestrations and the semantic breakdown of the service request and creation in real time. This enabled the research to draw observations that led to conclusions presented in this work. The research noted that the use of semantic technologies facilitates interoperability in heterogeneous devices and can be implemented as a means to bypass challenges presented by differences in IoT “things”.
- Full Text:
Effect of Moringa oleifera leaf meal on growth performance, faecal ammonia emissions, carcass characteristics and the quality of meat from broiler chickens
- Authors: Mxunyelwa, Avela,
- Date: 2016
- Subjects: Moringa oleifera Meat -- Quality Broilers (Chickens)
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/15034 , vital:40154
- Description: The objective of the study was to determine growth performance, faecal ammonia gas emission, carcass characteristics and physico-chemical meat traits from broilers fed one of the four dietary treatments, containing 0percent, 1percent, 3percent or 5percent Moringa oleifera leaf meal (MOLM) (T1, T2, T3, and T4, respectively). Dietary treatments were formulated to be isonitrogenous and isocaloric for a starter (0-14 days), grower (15-21days) and finisher (22-35 days). A total of 160 day old Cobb 500 broiler chickens, initially weighing an average of 45g were randomly allocated to each of the dietary treatments, each with four replicates, in 16 pens. Feed and water were offered ad libitum. Feed intakes (FI), average daily gain (ADG), body weight gain (BWG) and feed conversion efficiency (FCE) were measured weekly. At the end of the feeding trial, the feed was withdrawn from the birds for 12 hours with water available ad libitum and the birds were slaughtered using standard abattoir procedures. The breast muscle of each carcass was sampled for meat quality analysis. Organ and carcass weights differences were measured. Birds fed with T1 had the highest FI. Birds fed with T4 had the best FCE at day 14, 21 and 35. At day 7 and 35, there were no significant differences in FCE in all the treatments. At 0 to 35 days, faecal ammonia emission decreased (P<0.05) as MOLM concentration and age of birds increased. Moringa fed broilers had the highest body weights in all the periods despite the incorporation level in the diet. The results showed that inclusion of MOLM had a significant effect (P<0.05) on the heart, neck, and head weights but had no significant effect on liver, gizzard, intestine and feet. Broilers fed with T1 had heavier heart, neck and head weights compared to MOLM fed broilers. In terms of carcass traits, MOLM had a significant effect (P<0.05) on wings and thighs but had no significant effect (P>0.05) on breast and drumsticks. Moringa oleifera leaf meal had a significant effect on carcass weights, control diet had heavier weights (1185g ± 28.96) compared to chickens fed with MOLM diets (1121g±28.96, 1070g ± 28.96, 1030g± 28.96). Broilers fed with MOLM had higher values of lightness (L*) and (b*) values (P<0.05) at 45 minutes and 24 hours and no difference was observed in redness (a*) at 45 minutes and 24 hours. The L* values dropped at 24 hours for all the treatments and MOLM fed broilers had the lowest values (46.59 ± 0.76,46 ± 0.76,49 ± 0.76) compared to the control diet (50 ± 0.76). The pH levels were comparable after 24 hours; T2 had the highest pH values (5.95 ± 0.024) compared to other treatments (5.90 ± 0.024, 5.75 ± 0.024, 5.73 ± 0.024). The results of this study demonstrated that even though the FI was reduced in MOLM fed broilers, they efficiently consumed and used nutrients, as a result MOLM fed birds had higher weights and low ammonia concentration throughout the experiment, which may improve the house environment without affecting the quality of meat.
- Full Text:
- Authors: Mxunyelwa, Avela,
- Date: 2016
- Subjects: Moringa oleifera Meat -- Quality Broilers (Chickens)
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/15034 , vital:40154
- Description: The objective of the study was to determine growth performance, faecal ammonia gas emission, carcass characteristics and physico-chemical meat traits from broilers fed one of the four dietary treatments, containing 0percent, 1percent, 3percent or 5percent Moringa oleifera leaf meal (MOLM) (T1, T2, T3, and T4, respectively). Dietary treatments were formulated to be isonitrogenous and isocaloric for a starter (0-14 days), grower (15-21days) and finisher (22-35 days). A total of 160 day old Cobb 500 broiler chickens, initially weighing an average of 45g were randomly allocated to each of the dietary treatments, each with four replicates, in 16 pens. Feed and water were offered ad libitum. Feed intakes (FI), average daily gain (ADG), body weight gain (BWG) and feed conversion efficiency (FCE) were measured weekly. At the end of the feeding trial, the feed was withdrawn from the birds for 12 hours with water available ad libitum and the birds were slaughtered using standard abattoir procedures. The breast muscle of each carcass was sampled for meat quality analysis. Organ and carcass weights differences were measured. Birds fed with T1 had the highest FI. Birds fed with T4 had the best FCE at day 14, 21 and 35. At day 7 and 35, there were no significant differences in FCE in all the treatments. At 0 to 35 days, faecal ammonia emission decreased (P<0.05) as MOLM concentration and age of birds increased. Moringa fed broilers had the highest body weights in all the periods despite the incorporation level in the diet. The results showed that inclusion of MOLM had a significant effect (P<0.05) on the heart, neck, and head weights but had no significant effect on liver, gizzard, intestine and feet. Broilers fed with T1 had heavier heart, neck and head weights compared to MOLM fed broilers. In terms of carcass traits, MOLM had a significant effect (P<0.05) on wings and thighs but had no significant effect (P>0.05) on breast and drumsticks. Moringa oleifera leaf meal had a significant effect on carcass weights, control diet had heavier weights (1185g ± 28.96) compared to chickens fed with MOLM diets (1121g±28.96, 1070g ± 28.96, 1030g± 28.96). Broilers fed with MOLM had higher values of lightness (L*) and (b*) values (P<0.05) at 45 minutes and 24 hours and no difference was observed in redness (a*) at 45 minutes and 24 hours. The L* values dropped at 24 hours for all the treatments and MOLM fed broilers had the lowest values (46.59 ± 0.76,46 ± 0.76,49 ± 0.76) compared to the control diet (50 ± 0.76). The pH levels were comparable after 24 hours; T2 had the highest pH values (5.95 ± 0.024) compared to other treatments (5.90 ± 0.024, 5.75 ± 0.024, 5.73 ± 0.024). The results of this study demonstrated that even though the FI was reduced in MOLM fed broilers, they efficiently consumed and used nutrients, as a result MOLM fed birds had higher weights and low ammonia concentration throughout the experiment, which may improve the house environment without affecting the quality of meat.
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Effect of phosphorus application on dry matter yield and nutrient content of Mucuna Pruriens (L) in Alice
- Authors: Tshwati, Nolubabalo
- Date: 2016
- Subjects: Legumes Phosphorus Fertilizers
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/12326 , vital:39253
- Description: The use of herbaceous legumes such as Mucuna (Mucuna pruriens) can assist to recapitalize soil fertility depletion and improve livestock productivity in the small holder farming sector of South Africa. The objective of the study was to investigate the effect of phosphorus fertilizer application (0, 20, 40 and 60 kg P/ha) on the dry matter yield and nutritive value of Mucuna pruriens. The study was conducted at University of Fort Hare Research farm in Alice, South Africa. The research design was a randomized complete block design with three replicates per treatment. Data collection included biomass production and nutrient content of Mucuna pruriens. The forage legume was harvested at flowering stage of growth and analysed for chemical composition. The results showed that with the application of 60 kg P/ha Mucuna had the highest (P < 0.05) fresh and dry matter yield of 19.58 kg/ha and 5.41 kg/ha, respectively compared to other treatments. However, all the other treatments differed from one another. Similarly, the fresh stem and leaf fractions yield and their dry matter yields showed the same trend with application of 60 kg P/ha having the greatest yield compared to the other treatments. Nevertheless, all the treatments differed (P<0.05) from one another with P0 having the least yields. The leaf to stem ratio did not show any significant (P>0.05) difference among all the treatments ranging from 1.44 to 1.62 in fresh forage and ranging from 1.31 to 1.50 in the dry forage. In terms of dry matter content, the control treatment had the highest dry matter content of 37.78percent, however, all the other treatments did not differ significantly (P < 0.05) from each other. There was no significant effect of P application (P > 0.05) in terms of ash, EE, ADF, CP, Ca, Mg, K, Na, P, Zn, Cu, Mn and Fe of the whole plant forage among the treatments. The application of 40 kg P/ha fertilizer significantly (P < 0.05) increased the NDF (51.16percent) of the whole plant of Mucuna compared to the other treatments which did not differ (p>0.05) among themselves. Within the stem fraction there were no significant (P > 0.05) differences among the treatments for the ash, EE, ADF, NDF, and CP, hemicellulose, Ca, Mg, K, P, Zn, Cu, Mn and Fe contents. However, the application of 40 kg P/ha fertilizer significantly (P < 0.05) increased the Na (0.05percent) content of the Mucuna stem fraction. In the leaf fraction of Mucuna there was no significant (P > 0.05) differences in terms of ash, EE, ADF, NDF, CP, hemicellulose, Ca, K, P, Zn, Cu, Mn and Fe among the treatments. However, the application of 20 kg P/ha fertilizer significantly (P < 0.05) increased the Mg (0.52percent) of the Mucuna leaves compared to the other treatments. Therefore the study has shown that increase in P application (60 kg P/ha) has no major effect in the nutrient content of Mucuna. Furthermore, it is recommended that Mucuna can be grown as a protein supplement, based on the CP content values that were recorded in this study and to provide maintenance requirements for ruminants. It was therefore concluded that phosphorus fertilization particularly 60 kg P/ha is necessary and required for optimum growth of Mucuna pruriens in order to increase forage yield. Based on the nutrient content result it was concluded that there was no effect of P fertilizer application levels on the nutrient content of Mucuna pruriens in Alice.
- Full Text:
- Authors: Tshwati, Nolubabalo
- Date: 2016
- Subjects: Legumes Phosphorus Fertilizers
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/12326 , vital:39253
- Description: The use of herbaceous legumes such as Mucuna (Mucuna pruriens) can assist to recapitalize soil fertility depletion and improve livestock productivity in the small holder farming sector of South Africa. The objective of the study was to investigate the effect of phosphorus fertilizer application (0, 20, 40 and 60 kg P/ha) on the dry matter yield and nutritive value of Mucuna pruriens. The study was conducted at University of Fort Hare Research farm in Alice, South Africa. The research design was a randomized complete block design with three replicates per treatment. Data collection included biomass production and nutrient content of Mucuna pruriens. The forage legume was harvested at flowering stage of growth and analysed for chemical composition. The results showed that with the application of 60 kg P/ha Mucuna had the highest (P < 0.05) fresh and dry matter yield of 19.58 kg/ha and 5.41 kg/ha, respectively compared to other treatments. However, all the other treatments differed from one another. Similarly, the fresh stem and leaf fractions yield and their dry matter yields showed the same trend with application of 60 kg P/ha having the greatest yield compared to the other treatments. Nevertheless, all the treatments differed (P<0.05) from one another with P0 having the least yields. The leaf to stem ratio did not show any significant (P>0.05) difference among all the treatments ranging from 1.44 to 1.62 in fresh forage and ranging from 1.31 to 1.50 in the dry forage. In terms of dry matter content, the control treatment had the highest dry matter content of 37.78percent, however, all the other treatments did not differ significantly (P < 0.05) from each other. There was no significant effect of P application (P > 0.05) in terms of ash, EE, ADF, CP, Ca, Mg, K, Na, P, Zn, Cu, Mn and Fe of the whole plant forage among the treatments. The application of 40 kg P/ha fertilizer significantly (P < 0.05) increased the NDF (51.16percent) of the whole plant of Mucuna compared to the other treatments which did not differ (p>0.05) among themselves. Within the stem fraction there were no significant (P > 0.05) differences among the treatments for the ash, EE, ADF, NDF, and CP, hemicellulose, Ca, Mg, K, P, Zn, Cu, Mn and Fe contents. However, the application of 40 kg P/ha fertilizer significantly (P < 0.05) increased the Na (0.05percent) content of the Mucuna stem fraction. In the leaf fraction of Mucuna there was no significant (P > 0.05) differences in terms of ash, EE, ADF, NDF, CP, hemicellulose, Ca, K, P, Zn, Cu, Mn and Fe among the treatments. However, the application of 20 kg P/ha fertilizer significantly (P < 0.05) increased the Mg (0.52percent) of the Mucuna leaves compared to the other treatments. Therefore the study has shown that increase in P application (60 kg P/ha) has no major effect in the nutrient content of Mucuna. Furthermore, it is recommended that Mucuna can be grown as a protein supplement, based on the CP content values that were recorded in this study and to provide maintenance requirements for ruminants. It was therefore concluded that phosphorus fertilization particularly 60 kg P/ha is necessary and required for optimum growth of Mucuna pruriens in order to increase forage yield. Based on the nutrient content result it was concluded that there was no effect of P fertilizer application levels on the nutrient content of Mucuna pruriens in Alice.
- Full Text:
Effects of Pteronia incana (Blue bush) invasion on grass biomass production, soil chemical characteristics and peoples' livelihoods in Ngqushwa communal rangelands, Eastern Cape
- Authors: Ntutha, T
- Date: 2016
- Subjects: Invasive plants -- South Africa -- Eastern Cape Shrublands -- South Africa -- Eastern Cape Rangelands -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/4682 , vital:28493
- Description: The objective of the study was to assess the farmers’ knowledge and perceptions of P. incana invasion and impacts on rangeland, livestock production and livelihood in four communal grazing areas. Twenty households per village that own livestock were randomly selected to conduct an interview using an open and closed ended questionnaire. In each household, an old and knowledgeable respondent about P. incana invasion was used as a selection criterion. For scientific assessment of range condition, the experiment was conducted in a 270 m x 100 m trial plots that were established in 2014. The area was divided into 3 open and 3 fenced subplots each having an area of 45 m x 100 m established laid down a slope gradient. Each plot was divided into four equal subplots to apply the control treatments. Therefore, the trial layout was a stratified block experiment of four treatments replicated three times. The four control treatments applied to P. incana invasion were chopping and resting (CR), resting without chopping (RWOC), grazing and chopping (GC), grazing without chopping (GWOC). A step point method was applied in each transect to monitor species composition and P. incana cover. On the fenced plots two exclosures of 1mx1m size were randomly distributed to make sure that resting is maintained. Within exclosures 0.25 m2quadrates in each were put randomly to measure biomass. Density of P. incana was determined from 5 m x 5 m quadrates that were placed randomly within each sub-plot. The density and height of individual shrub plant was measured. All rooted live woody plants were recorded and counted in each quadrate. The results revealed that species composition was not affected (P>0.05) by the treatments applied. The herbage height and basal cover had greater values (P<0.05) under rested plus chopping (CR) treatment followed by grazed plus chopping (GC). Grass biomass production and essential nutrients showed a greater concentration (P<0.05) under CR treatment than any treatments applied. The results on perceptions revealed that goats had the highest mean household holding number than any other livestock species across the selected villages in all the villages. Respondents indicated that both goats and cattle had equal or more importance than sheep. Three out of four villages raised cattle primarily for income generation and secondarily for cultural purposes like slaughtering during weddings ceremonies, amazila and also during woman circumcisions (Ntonjane). Similarly, goats and sheep were primarily raised for cash income generation. The results revealed that livestock population decreased over ten to twenty years (one-two decades) and poor rangeland condition is assumed to be the cause of the situation as feed is the key factor for production. The area was dominated by the grasses rather than by browse woody species so the above results are possible as the P. incana is more competitive than grasses. It was concluded that P. incana is detrimental to both rangeland and animal production. Forage and animal performance as well as farmers’ economic status (livelihoods) were negatively affected by P. incana invasion and the scientific assessment reported it to be detrimental to agriculture. Thus chopping and resting (CR) treatment resulted in a greater improvement of the basal cover, biomass production, soil nutrients as well as grass height than the other treatments applied. Therefore it can be concluded that chopping and resting the veld invaded with P. incana can improve the range and consequently animal production.
- Full Text:
- Authors: Ntutha, T
- Date: 2016
- Subjects: Invasive plants -- South Africa -- Eastern Cape Shrublands -- South Africa -- Eastern Cape Rangelands -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/4682 , vital:28493
- Description: The objective of the study was to assess the farmers’ knowledge and perceptions of P. incana invasion and impacts on rangeland, livestock production and livelihood in four communal grazing areas. Twenty households per village that own livestock were randomly selected to conduct an interview using an open and closed ended questionnaire. In each household, an old and knowledgeable respondent about P. incana invasion was used as a selection criterion. For scientific assessment of range condition, the experiment was conducted in a 270 m x 100 m trial plots that were established in 2014. The area was divided into 3 open and 3 fenced subplots each having an area of 45 m x 100 m established laid down a slope gradient. Each plot was divided into four equal subplots to apply the control treatments. Therefore, the trial layout was a stratified block experiment of four treatments replicated three times. The four control treatments applied to P. incana invasion were chopping and resting (CR), resting without chopping (RWOC), grazing and chopping (GC), grazing without chopping (GWOC). A step point method was applied in each transect to monitor species composition and P. incana cover. On the fenced plots two exclosures of 1mx1m size were randomly distributed to make sure that resting is maintained. Within exclosures 0.25 m2quadrates in each were put randomly to measure biomass. Density of P. incana was determined from 5 m x 5 m quadrates that were placed randomly within each sub-plot. The density and height of individual shrub plant was measured. All rooted live woody plants were recorded and counted in each quadrate. The results revealed that species composition was not affected (P>0.05) by the treatments applied. The herbage height and basal cover had greater values (P<0.05) under rested plus chopping (CR) treatment followed by grazed plus chopping (GC). Grass biomass production and essential nutrients showed a greater concentration (P<0.05) under CR treatment than any treatments applied. The results on perceptions revealed that goats had the highest mean household holding number than any other livestock species across the selected villages in all the villages. Respondents indicated that both goats and cattle had equal or more importance than sheep. Three out of four villages raised cattle primarily for income generation and secondarily for cultural purposes like slaughtering during weddings ceremonies, amazila and also during woman circumcisions (Ntonjane). Similarly, goats and sheep were primarily raised for cash income generation. The results revealed that livestock population decreased over ten to twenty years (one-two decades) and poor rangeland condition is assumed to be the cause of the situation as feed is the key factor for production. The area was dominated by the grasses rather than by browse woody species so the above results are possible as the P. incana is more competitive than grasses. It was concluded that P. incana is detrimental to both rangeland and animal production. Forage and animal performance as well as farmers’ economic status (livelihoods) were negatively affected by P. incana invasion and the scientific assessment reported it to be detrimental to agriculture. Thus chopping and resting (CR) treatment resulted in a greater improvement of the basal cover, biomass production, soil nutrients as well as grass height than the other treatments applied. Therefore it can be concluded that chopping and resting the veld invaded with P. incana can improve the range and consequently animal production.
- Full Text:
Environmental factors influencing ecotonal changes in an indigenous forest in the Keiskammahoek Forest Estate, Eastern Cape, South Africa
- Authors: Kiva, Luthando
- Date: 2016
- Subjects: Forest ecology -- South Africa -- Eastern Cape Forests and forestry -- South Africa -- Eastern Cape Forest conservation -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/2743 , vital:28071
- Description: This study investigates environmental factors influencing ecotonal changes in the Dontsa Forest Management Unit (FMU) of the Keiskammahoek Estate Forest which is located in the Amatole Mountains of the Eastern Cape in the Republic of South Africa. The patterns of forest edge movement were analysed to show trends of forest edge movement from 1975 to 1985, 1975 to 1992, 1975 to 2002, 1975 to 2014, 1985 to 1992, 1985 to 2002, 1985 to 2014, 1992 to 2002, 1992 to 2014 and 2002 to 2014 by digitizing and assessing the scale of forest edge movement using georeferenced aerial photographs. Belt transects were established in six sites that were selected on the basis of physiographic elements for determination of the driving forces of forest ecotonal changes. The results of the study show that the forest edge moved positive towards the grassland biome while in other sites there was contraction of the forest edge from 1975 to 2014. The findings of the study show that some forest patches moved with few individual pioneer species towards the grassland while indigenous species dominated in the ecotone area of the forest in other research sites. D whyteana, A latifolius, R melanophloes, A facultus, R prenoides, C aurea, C bispinosa, C inerme, and S martina are the plant species with high density in the forest ecotone while A latifolius, R prenoides, R melanophloes were highly distributed along the grassland area. The results also show that harvesting of Pinus patula and illegal harvesting of understory species are major factors that result in ecotonal changes of Dontsa FMU. The research sites adjacent to residential areas have experienced reduction of ecotone area as compared to the research sites in high altitude areas of the Amathole Mountains where there is less disturbance. The eastern facing aspect of the forest exhibited a high density of plants in the forest ecotone as compared to the west facing slope.
- Full Text:
- Authors: Kiva, Luthando
- Date: 2016
- Subjects: Forest ecology -- South Africa -- Eastern Cape Forests and forestry -- South Africa -- Eastern Cape Forest conservation -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/2743 , vital:28071
- Description: This study investigates environmental factors influencing ecotonal changes in the Dontsa Forest Management Unit (FMU) of the Keiskammahoek Estate Forest which is located in the Amatole Mountains of the Eastern Cape in the Republic of South Africa. The patterns of forest edge movement were analysed to show trends of forest edge movement from 1975 to 1985, 1975 to 1992, 1975 to 2002, 1975 to 2014, 1985 to 1992, 1985 to 2002, 1985 to 2014, 1992 to 2002, 1992 to 2014 and 2002 to 2014 by digitizing and assessing the scale of forest edge movement using georeferenced aerial photographs. Belt transects were established in six sites that were selected on the basis of physiographic elements for determination of the driving forces of forest ecotonal changes. The results of the study show that the forest edge moved positive towards the grassland biome while in other sites there was contraction of the forest edge from 1975 to 2014. The findings of the study show that some forest patches moved with few individual pioneer species towards the grassland while indigenous species dominated in the ecotone area of the forest in other research sites. D whyteana, A latifolius, R melanophloes, A facultus, R prenoides, C aurea, C bispinosa, C inerme, and S martina are the plant species with high density in the forest ecotone while A latifolius, R prenoides, R melanophloes were highly distributed along the grassland area. The results also show that harvesting of Pinus patula and illegal harvesting of understory species are major factors that result in ecotonal changes of Dontsa FMU. The research sites adjacent to residential areas have experienced reduction of ecotone area as compared to the research sites in high altitude areas of the Amathole Mountains where there is less disturbance. The eastern facing aspect of the forest exhibited a high density of plants in the forest ecotone as compared to the west facing slope.
- Full Text:
Evaluating farmers' perceptions and the impact of bush encroachment on herbaceous vegetation and soil nutrients in Sheshegu communal rangelands of the Eastern Cape, South Africa
- Authors: Tokozwayo, Sive
- Date: 2016
- Subjects: Range management -- South Africa -- Eastern Cape Brush -- Control -- South Africa -- Eastern Cape Communal rangelands -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/2733 , vital:28066
- Description: Communal rangelands occupy 13 percent of the agricultural land in South Africa, and these rangelands serve as a source of feed to livestock. These areas are threatened by bush encroachment due to poor rangeland management. This study was conducted in Alice (Sheshegu communal area) and the objective was to assess farmers' perceptions and the impact of bush encroachment on herbaceous vegetation and soil nutrients. Structured questionnaires were used to assess indigenous knowledge of communal farmers on the impact of bush encroachment on rangelands. Fourty (40) respondents from households who owned livestock were randomly selected and interviewed at Sheshegu village. About 89 percent of communal farmers perceived that change of their grasslands to encroached savannas was caused by unreliable rainfall, prolonged drought, and poor rangeland management. Rangeland assessment was performed at four sites (Scattered, Moderate, Mixed and Dense bushland. 100mx50m were demarcated per site, and four 100m transects were laid parallel to each other, 30m apart. The step point method was used to determine both species composition and basal cover. Biomass production was determined by harvesting forage within randomly-paced a 0.25m2 quadrats. Aristada congesta and Eragrostis obtuse were the most dominating grass species in dense, mixed and moderate bushland. Scattered bushland was dominated by Themeda triandra. Biomass production increases with increase in bush density and basal cover improved from winter to summer season. Increaser grass species increase with the increase in bush density, this indicated that the rangeland was poorly managed and palatable species were being replaced by less palatable ones. Species composition of woody plants was determined within a 200m2 belt transect in all sites. Maytenus polycantha, Aloe ferox, Erehia rigida and lucas capensis were the most dominant woody species in Mixed bushland while Acacia karroo was most dominated in Scattered, Moderate and Dense bushland. Woody density showed no significant differences (P> 0.05) between Dense, Mixed and Moderate bushland. Woody plant density in all these sites exceed 2500 plants/ha, which showed that the rangeland was encroached with woody plants. Soil nutrient content was determined for each site. Five samples of soil were collected per site to test the effect of bush encroachment on soil nutrient composition. The concentration of nitrogen and soil pH increased with increase in bush density, while that of potassium, phosphorous, magnesium, sodium and sulphur showed no significant differences (P>0.05) across all homogenous vegetation units. The study revealed that Sheshegu communal farmers are aware of both negative and positive effects of encroachment on their community, and also argue that the government is not willing to support communal farmers in the process of reducing the spread of bush encroachment. It was concluded that the concentration of nitrogen and soil pH increased with increasing bush density and that bush encroachment had negative effects on herbaceous species. It was recommended that communal farmers should be formulating rangeland rules and regulations to manage rangeland resources. Rangeland rules and regulations should be based on the management of livestock management during grazing and harvesting of rangeland resources. The harvesting of woody and good timing of application of fire can also reduce the spread of bush encroachment. Veld resting and rotational grazing can also improve fuel load which is required for burning of woody plants. Furthermore, Department of agriculture should also provide workshops or training to communal farmers use different approaches or cheap ways of reducing bush encroachment and conserving rangeland resources.
- Full Text:
- Authors: Tokozwayo, Sive
- Date: 2016
- Subjects: Range management -- South Africa -- Eastern Cape Brush -- Control -- South Africa -- Eastern Cape Communal rangelands -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/2733 , vital:28066
- Description: Communal rangelands occupy 13 percent of the agricultural land in South Africa, and these rangelands serve as a source of feed to livestock. These areas are threatened by bush encroachment due to poor rangeland management. This study was conducted in Alice (Sheshegu communal area) and the objective was to assess farmers' perceptions and the impact of bush encroachment on herbaceous vegetation and soil nutrients. Structured questionnaires were used to assess indigenous knowledge of communal farmers on the impact of bush encroachment on rangelands. Fourty (40) respondents from households who owned livestock were randomly selected and interviewed at Sheshegu village. About 89 percent of communal farmers perceived that change of their grasslands to encroached savannas was caused by unreliable rainfall, prolonged drought, and poor rangeland management. Rangeland assessment was performed at four sites (Scattered, Moderate, Mixed and Dense bushland. 100mx50m were demarcated per site, and four 100m transects were laid parallel to each other, 30m apart. The step point method was used to determine both species composition and basal cover. Biomass production was determined by harvesting forage within randomly-paced a 0.25m2 quadrats. Aristada congesta and Eragrostis obtuse were the most dominating grass species in dense, mixed and moderate bushland. Scattered bushland was dominated by Themeda triandra. Biomass production increases with increase in bush density and basal cover improved from winter to summer season. Increaser grass species increase with the increase in bush density, this indicated that the rangeland was poorly managed and palatable species were being replaced by less palatable ones. Species composition of woody plants was determined within a 200m2 belt transect in all sites. Maytenus polycantha, Aloe ferox, Erehia rigida and lucas capensis were the most dominant woody species in Mixed bushland while Acacia karroo was most dominated in Scattered, Moderate and Dense bushland. Woody density showed no significant differences (P> 0.05) between Dense, Mixed and Moderate bushland. Woody plant density in all these sites exceed 2500 plants/ha, which showed that the rangeland was encroached with woody plants. Soil nutrient content was determined for each site. Five samples of soil were collected per site to test the effect of bush encroachment on soil nutrient composition. The concentration of nitrogen and soil pH increased with increase in bush density, while that of potassium, phosphorous, magnesium, sodium and sulphur showed no significant differences (P>0.05) across all homogenous vegetation units. The study revealed that Sheshegu communal farmers are aware of both negative and positive effects of encroachment on their community, and also argue that the government is not willing to support communal farmers in the process of reducing the spread of bush encroachment. It was concluded that the concentration of nitrogen and soil pH increased with increasing bush density and that bush encroachment had negative effects on herbaceous species. It was recommended that communal farmers should be formulating rangeland rules and regulations to manage rangeland resources. Rangeland rules and regulations should be based on the management of livestock management during grazing and harvesting of rangeland resources. The harvesting of woody and good timing of application of fire can also reduce the spread of bush encroachment. Veld resting and rotational grazing can also improve fuel load which is required for burning of woody plants. Furthermore, Department of agriculture should also provide workshops or training to communal farmers use different approaches or cheap ways of reducing bush encroachment and conserving rangeland resources.
- Full Text:
Evaluation of physiochemical qualities and heavy metal levels of the final effluents of some wastewater treatment facilities in the Eastern Cape Province of South Africa
- Authors: Kulati, Thanduxolo Cullinan
- Date: 2016
- Subjects: Water -- Purification Sewage -- Purification
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/1547 , vital:27417
- Description: Water is the most abundant substance in nature and vital for life activities. The major water sources for use are surface water bodies such as rivers and lakes, and underground aquifers and pore spaces down the water table (Ring, 2003). Water derived from these sources is not necessarily pure since it contains dissolved inorganic and organic substances, living organisms (viruses, bacteria, etc). For these reasons, water intended for domestic uses should be free from toxic substances and microorganisms that are of health significance (WHO, 2005). The availability and quality of water always have played an important role in determining the quality of life. Water quality is closely linked to water use and to the state of economic development (Chennakrishnan et al., 2008). Ground and surface waters can be contaminated by several sources. In urban areas, the careless disposal of industrial effluents and other wastes may contribute greatly to the poor quality of water (Mathuthu et al., 1997). In most developing countries, most areas are located on the watersheds which are the end points of effluents discharged from various industries (Oberholster and Ashton, 2008). South Africa, as a developing country, is experiencing rapid demographic changes due to urbanization, industrialization and population growth. The country has also been identified as being water-scarce, which can lead to a challenge of meeting the increasing water demand due to industrialization and urbanization. Such population growth increase may result in an increase in wastewater output, especially around urban areas.
- Full Text:
- Authors: Kulati, Thanduxolo Cullinan
- Date: 2016
- Subjects: Water -- Purification Sewage -- Purification
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/1547 , vital:27417
- Description: Water is the most abundant substance in nature and vital for life activities. The major water sources for use are surface water bodies such as rivers and lakes, and underground aquifers and pore spaces down the water table (Ring, 2003). Water derived from these sources is not necessarily pure since it contains dissolved inorganic and organic substances, living organisms (viruses, bacteria, etc). For these reasons, water intended for domestic uses should be free from toxic substances and microorganisms that are of health significance (WHO, 2005). The availability and quality of water always have played an important role in determining the quality of life. Water quality is closely linked to water use and to the state of economic development (Chennakrishnan et al., 2008). Ground and surface waters can be contaminated by several sources. In urban areas, the careless disposal of industrial effluents and other wastes may contribute greatly to the poor quality of water (Mathuthu et al., 1997). In most developing countries, most areas are located on the watersheds which are the end points of effluents discharged from various industries (Oberholster and Ashton, 2008). South Africa, as a developing country, is experiencing rapid demographic changes due to urbanization, industrialization and population growth. The country has also been identified as being water-scarce, which can lead to a challenge of meeting the increasing water demand due to industrialization and urbanization. Such population growth increase may result in an increase in wastewater output, especially around urban areas.
- Full Text:
Fatty acid composition, colour stability and lipid oxidation of mince produced from fresh and frozen/thawed fallow deer meat
- Authors: Chido, Chakanya
- Date: 2016
- Subjects: Lipids -- Oxidation Meat -- Preservation Mincemeat
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/2479 , vital:27874
- Description: The aim of the study was to determine the fatty acid composition, colour stability and lipid oxidation of fresh mince produced from fallow deer and to evaluate the effect of frozen storage duration on the retail display shelf life of the mince. A total of 31 fallow deer carcasses were used in the study. After cooling for 24hrs, the carcasses were deboned, external fat from the fore and hindquarter muscles removed and individually vacuum packed. For the first trial, seven fallow deer carcasses were used. Meat from the hind and fore-quarters of each carcass was divided into two equal batches per animal. One batch was minced (through a 5 mm die) and packed into oxygen permeable overwraps and refrigerated at 4°C for a period of eight days under retail display conditions. The second batch was vacuum packed and frozen at -20°C for 2 months at the end of which mince was also produced and monitored over an eight-day period under the same conditions that were used for the fresh mince. Colour, pH, lipid and myoglobin stability was determined. Proximate and fatty acid composition was also determined. No differences (P>0.05) were noted between proximate composition of fresh and frozen/thawed minced meat. The lipid content of fallow deer was 2.4 percent (±0.04). Total n3 fatty acids differed (P<0.05) between treatments and decreased with increased storage and display day. There were significant (P<0.05) treatment and time interactions on all measured colour parameters, TBARS and myoglobin forms. Fresh mince was lighter and had higher redness (a*) and yellowness (b*) values than mince from two months frozen stored meat. Hue angle for fresh mince remained stable throughout display whereas it increased for frozen/thawed mince. Fresh mince had lower TBARS values than frozen/thawed mince. Minced meat produced from frozen/thawed deer meat had higher surface met-myoglobin and total met-myoglobin percentages. Surface and total oxy-myoglobin percentage was higher in fresh mince. The first trial clearly showed colour and lipid stability differences between fresh mince and mince from frozen/thawed meat. It also showed that fresh mince has a longer retail display life than mince produced from frozen/thawed meat (six days and four days, respectively). In the second trial, the effects of frozen storage duration on colour and lipid stability were investigated. Twenty-four fallow deer were used. Twelve were harvested in June (6male 6female) and the other twelve in August (6 male 6female) of the same year.Twenty four hours after harvesting, the fore and hindquarter muscles of the carcasses were deboned, vacuum packed and kept at -20°C until October (i.e. 2months and 4months frozen storage period). Upon thawing, the meat was processed into mince following the same procedure used for the first trialand displayed for a fiveday period under retail display conditions. Frozen duration and gender had no effect (P>0.05) on the proximate composition of fallow deer meat. The total amount of saturated fatty acids (SFA) increased and total amount of poly unsaturated fatty acids (PUFA) decreased as frozen duration and display day increased (P<0.05). Frozen duration affected (P<0.01) lipid oxidation and percentage oxy-myoglobin. Mince pH and all colour parameters (L*, a*, b*,hue and chroma) differed (P<0.05) between treatments on day zero and three. Display day was a significant factor (P<0.05) on all measured parameters. By day three all parameters except pH showed signs of extended oxidation and discolouration as evidenced by reduced redness, decreased colour intensity and high TBARS values. This study showed that prolonged frozen storage negatively affects the colour and lipid stability of meat and increases oxidation of PUFAs during frozen storage. However, the study also suggests that although frozen/thawed meat has a shorter retail display shelf life; the proximate composition of the meat remains unchanged.
- Full Text:
- Authors: Chido, Chakanya
- Date: 2016
- Subjects: Lipids -- Oxidation Meat -- Preservation Mincemeat
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/2479 , vital:27874
- Description: The aim of the study was to determine the fatty acid composition, colour stability and lipid oxidation of fresh mince produced from fallow deer and to evaluate the effect of frozen storage duration on the retail display shelf life of the mince. A total of 31 fallow deer carcasses were used in the study. After cooling for 24hrs, the carcasses were deboned, external fat from the fore and hindquarter muscles removed and individually vacuum packed. For the first trial, seven fallow deer carcasses were used. Meat from the hind and fore-quarters of each carcass was divided into two equal batches per animal. One batch was minced (through a 5 mm die) and packed into oxygen permeable overwraps and refrigerated at 4°C for a period of eight days under retail display conditions. The second batch was vacuum packed and frozen at -20°C for 2 months at the end of which mince was also produced and monitored over an eight-day period under the same conditions that were used for the fresh mince. Colour, pH, lipid and myoglobin stability was determined. Proximate and fatty acid composition was also determined. No differences (P>0.05) were noted between proximate composition of fresh and frozen/thawed minced meat. The lipid content of fallow deer was 2.4 percent (±0.04). Total n3 fatty acids differed (P<0.05) between treatments and decreased with increased storage and display day. There were significant (P<0.05) treatment and time interactions on all measured colour parameters, TBARS and myoglobin forms. Fresh mince was lighter and had higher redness (a*) and yellowness (b*) values than mince from two months frozen stored meat. Hue angle for fresh mince remained stable throughout display whereas it increased for frozen/thawed mince. Fresh mince had lower TBARS values than frozen/thawed mince. Minced meat produced from frozen/thawed deer meat had higher surface met-myoglobin and total met-myoglobin percentages. Surface and total oxy-myoglobin percentage was higher in fresh mince. The first trial clearly showed colour and lipid stability differences between fresh mince and mince from frozen/thawed meat. It also showed that fresh mince has a longer retail display life than mince produced from frozen/thawed meat (six days and four days, respectively). In the second trial, the effects of frozen storage duration on colour and lipid stability were investigated. Twenty-four fallow deer were used. Twelve were harvested in June (6male 6female) and the other twelve in August (6 male 6female) of the same year.Twenty four hours after harvesting, the fore and hindquarter muscles of the carcasses were deboned, vacuum packed and kept at -20°C until October (i.e. 2months and 4months frozen storage period). Upon thawing, the meat was processed into mince following the same procedure used for the first trialand displayed for a fiveday period under retail display conditions. Frozen duration and gender had no effect (P>0.05) on the proximate composition of fallow deer meat. The total amount of saturated fatty acids (SFA) increased and total amount of poly unsaturated fatty acids (PUFA) decreased as frozen duration and display day increased (P<0.05). Frozen duration affected (P<0.01) lipid oxidation and percentage oxy-myoglobin. Mince pH and all colour parameters (L*, a*, b*,hue and chroma) differed (P<0.05) between treatments on day zero and three. Display day was a significant factor (P<0.05) on all measured parameters. By day three all parameters except pH showed signs of extended oxidation and discolouration as evidenced by reduced redness, decreased colour intensity and high TBARS values. This study showed that prolonged frozen storage negatively affects the colour and lipid stability of meat and increases oxidation of PUFAs during frozen storage. However, the study also suggests that although frozen/thawed meat has a shorter retail display shelf life; the proximate composition of the meat remains unchanged.
- Full Text:
Genotypic and phenotypic characterization of enterococci from cow dung and environmental water sources in three selected dairy farms in Amathole District
- Authors: Tanih, Godfred Ngu
- Date: 2016
- Subjects: Enterobacteriaceae Water -- Microbiology Enterococcus faecalis
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/2348 , vital:27755
- Description: Enterococcus species are integral members of the gastrointestinal microfloral of humans, animals, birds, as well as insects. Their presence in water and food has been greatly associated with faecal contamination. This study was aimed at evaluating the incidence of Enterococcus species in cow dung and environmental water sources in three commercial dairy farms. In addition, their antibiotic profiles were determined as well as resistance and virulence genes. Furthermore, the genetic relatedness of the isolates was determined by molecular typing method (RAPD PCR). Three hundred and thirty four water and faecal samples consisting of 117, 116 and 101 were collected from Seven Star Middle Drift and Fort Hare Dairy trusts respectively. Of the 334 samples collected, 289 were of faecal origin and 45 from water sources within the farms. All samples were screened for enterococci using culture base growth media and molecular methods targeting the tuf gene. Speciation was done using species-specific primers and the incidences of various species within the farms determined. Furthermore resistance to antibiotics and multidrug-resistant phenotypes were established using the disk diffusion method. Genes coding for virulence and resistance were also determined. From the samples collected, 313 (289 faecal and 24 water) presumptive enteroccocci were isolated, 305 of 313 (97.45 percent) were confirmed as Enterococcus of which 239 of 305 (78.38 percent) were identified as E. hirae, 15 of 305 (4.92 percent) as E. faecium, 12/305 (3.93 percent) as E. durans, 6 of 305 (1.97 percent) as E. faecalis and 33 of 305 (10.82 percent) were unidentified. Out of the five virulence genes that were targeted in the study only gelE (71.80 percent of 219/305) and ace (27.2 percent 83/305) were present in the isolates. Phenotypic resistance to antibiotics was observed is in all twelve antibiotics tested with multidrug resistance phenotypes detected in some enterococcal isolates most predominant in Seven Star and Middledrift dairy trust. Finally RAPD profiles of the isolates showed high relatedness between the strains from water and cow dung sources in all three commercial dairy farms suggesting possible contamination from cow dung to the water sources or vice versa.
- Full Text:
- Authors: Tanih, Godfred Ngu
- Date: 2016
- Subjects: Enterobacteriaceae Water -- Microbiology Enterococcus faecalis
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/2348 , vital:27755
- Description: Enterococcus species are integral members of the gastrointestinal microfloral of humans, animals, birds, as well as insects. Their presence in water and food has been greatly associated with faecal contamination. This study was aimed at evaluating the incidence of Enterococcus species in cow dung and environmental water sources in three commercial dairy farms. In addition, their antibiotic profiles were determined as well as resistance and virulence genes. Furthermore, the genetic relatedness of the isolates was determined by molecular typing method (RAPD PCR). Three hundred and thirty four water and faecal samples consisting of 117, 116 and 101 were collected from Seven Star Middle Drift and Fort Hare Dairy trusts respectively. Of the 334 samples collected, 289 were of faecal origin and 45 from water sources within the farms. All samples were screened for enterococci using culture base growth media and molecular methods targeting the tuf gene. Speciation was done using species-specific primers and the incidences of various species within the farms determined. Furthermore resistance to antibiotics and multidrug-resistant phenotypes were established using the disk diffusion method. Genes coding for virulence and resistance were also determined. From the samples collected, 313 (289 faecal and 24 water) presumptive enteroccocci were isolated, 305 of 313 (97.45 percent) were confirmed as Enterococcus of which 239 of 305 (78.38 percent) were identified as E. hirae, 15 of 305 (4.92 percent) as E. faecium, 12/305 (3.93 percent) as E. durans, 6 of 305 (1.97 percent) as E. faecalis and 33 of 305 (10.82 percent) were unidentified. Out of the five virulence genes that were targeted in the study only gelE (71.80 percent of 219/305) and ace (27.2 percent 83/305) were present in the isolates. Phenotypic resistance to antibiotics was observed is in all twelve antibiotics tested with multidrug resistance phenotypes detected in some enterococcal isolates most predominant in Seven Star and Middledrift dairy trust. Finally RAPD profiles of the isolates showed high relatedness between the strains from water and cow dung sources in all three commercial dairy farms suggesting possible contamination from cow dung to the water sources or vice versa.
- Full Text:
Heavy mineral characterization and provenance interpretation of the Ecca Group of geological formations in Eastern Cape Province, South Africa
- Authors: Sinuka, Sikhulule
- Date: 2016
- Subjects: Minerals -- South Africa -- Eastern Cape -- Classification Geology -- South Africa -- Eastern Cape Formations (Geology) -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/2911 , vital:28125
- Description: The aim of the research focuses on characterizing heavy mineral assemblages and interpretation of the provenance of the Ecca Group of in the Eastern Cape Province, South Africa. In South Africa, the Ecca Group outcrops extensively in the Main Karoo Basin. Mudstone, siltstone, sandstone, minor conglomerate and coal are the major constituent lithologies within the group. For descriptive purposes, the Ecca is categorized into three different geographical areas: the southern area, the western and northwestern area and the northeastern area. Six of the sixteen geological formations, namely the Prince Albert, Whitehill, Collingham, Ripon, Fort Brown, Waterford and Koonap Formations are present in the study area and are best exposed in road cuttings. For purposes of comparison, the underlying Witteberg Group, the Dwyka (which has Formation status here), and the overlying Koonap Formation of the Beaufort Group, are included in the study. This study is motivated by the relatively little information that is available on the heavy minerals of the Ecca Group, and that research of this nature had not been undertaken in the study area before. Another contributing motivation was to determine whether heavy mineral assemblages could be used to identify formations of the Ecca Group and for correlating between different localities in accordance with studies done elsewhere. Additionally, diagnostic heavy mineral assemblages could aid with stratigraphic selection of future boreholes in the Ecca Group. Heavy minerals are natural provenance tracers because of their stable nature and hydrodynamic behaviour. They are both non-opaque and opaque, with apatite, epidote, garnet, rutile, staurolite, tourmaline and zircon being good examples of non-opaque grains while ilmenite and magnetite are the most common opaques. Heavies are either derived from stable minor accessory minerals or from abundant but unstable mafic components of the host rock. They are very useful in interpreting the provenance due to the fact that some minerals are diagnostic of certain source rocks. However, sediments are exposed to several factors (conditions) such as weathering, erosion, breakage due to abrasion, mixing and recycling during transportation from the source to the depositional area. This implies that there are parameters other than the parent lithology that determine their final composition.
- Full Text:
- Authors: Sinuka, Sikhulule
- Date: 2016
- Subjects: Minerals -- South Africa -- Eastern Cape -- Classification Geology -- South Africa -- Eastern Cape Formations (Geology) -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/2911 , vital:28125
- Description: The aim of the research focuses on characterizing heavy mineral assemblages and interpretation of the provenance of the Ecca Group of in the Eastern Cape Province, South Africa. In South Africa, the Ecca Group outcrops extensively in the Main Karoo Basin. Mudstone, siltstone, sandstone, minor conglomerate and coal are the major constituent lithologies within the group. For descriptive purposes, the Ecca is categorized into three different geographical areas: the southern area, the western and northwestern area and the northeastern area. Six of the sixteen geological formations, namely the Prince Albert, Whitehill, Collingham, Ripon, Fort Brown, Waterford and Koonap Formations are present in the study area and are best exposed in road cuttings. For purposes of comparison, the underlying Witteberg Group, the Dwyka (which has Formation status here), and the overlying Koonap Formation of the Beaufort Group, are included in the study. This study is motivated by the relatively little information that is available on the heavy minerals of the Ecca Group, and that research of this nature had not been undertaken in the study area before. Another contributing motivation was to determine whether heavy mineral assemblages could be used to identify formations of the Ecca Group and for correlating between different localities in accordance with studies done elsewhere. Additionally, diagnostic heavy mineral assemblages could aid with stratigraphic selection of future boreholes in the Ecca Group. Heavy minerals are natural provenance tracers because of their stable nature and hydrodynamic behaviour. They are both non-opaque and opaque, with apatite, epidote, garnet, rutile, staurolite, tourmaline and zircon being good examples of non-opaque grains while ilmenite and magnetite are the most common opaques. Heavies are either derived from stable minor accessory minerals or from abundant but unstable mafic components of the host rock. They are very useful in interpreting the provenance due to the fact that some minerals are diagnostic of certain source rocks. However, sediments are exposed to several factors (conditions) such as weathering, erosion, breakage due to abrasion, mixing and recycling during transportation from the source to the depositional area. This implies that there are parameters other than the parent lithology that determine their final composition.
- Full Text:
Impact of smallholder irrigation schemes on rural livelihoods of farmers in the Eastern Cape Province of South Africa: the case of Melani Irrigation Scheme
- Authors: Magqibelo, Sinovuyo
- Date: 2016
- Subjects: Irrigation farming -- Economic aspects Irrigation -- South Africa -- Eastern Cape Farms, Small -- South Africa -- Eastern Cape , Rural development -- South Africa -- Eastern Cape Agriculture -- Economic aspects -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/1774 , vital:27557
- Description: Irrigation farming is one of the most important rural development investments that can have both direct and indirect impacts on poverty and food security. The irregular and unreliable rainfall in the Eastern Cape Province has caused the smallholder farmers to deepen the irrigation farming systems as a strategy to cope with food shortage, income and poverty. South Africa has invested substantially in smallholder irrigation to benefit smallholder farmers in the less developed areas. Although the investment on small scale irrigation was to improve the welfare of the people, the performance and welfare impact of smallholder irrigation schemes has been poor and fall far short of the expectations of many stakeholders. The broad objective of the study was to examine the contribution of smallholder irrigation scheme on rural livelihoods of smallholder farmers at the project area. The null hypothesis was that smallholder irrigation schemes have no impact on farmer’s livelihoods. The alternative hypothesis for this study was that Melani irrigation scheme is contributing in improving rural livelihoods of smallholder farmers in the area. Cross study research design was used in the methodology. The purposive sampling was applied in order to choose a sample of 107 respondents that were interviewed using questionnaires, including both irrigating and non-irrigating farmers. A multiple linear regression analysis was used to find out whether the performance of the two groups of farmers is different and to what extent. To determine the specific role of irrigation an Independent T-Test was ran. Out of the selected sample 68 percent of households were farmers, 7.5 percent informal employed and 27 percent unemployed. It was found that the majority of participants were males and were uneducated. They depended on social grants for a living. The findings from the study showed that 84 percent of the respondents agreed that there is no market available in the village and farmers had limited access to credits. The crop income was the most contributing factor to household income. The results showed that whether the farmer is a non-irrigator or an irrigator the performance is not significant. Irrigation scheme improvement attained by smallholder farmers need to be maintained.
- Full Text:
- Authors: Magqibelo, Sinovuyo
- Date: 2016
- Subjects: Irrigation farming -- Economic aspects Irrigation -- South Africa -- Eastern Cape Farms, Small -- South Africa -- Eastern Cape , Rural development -- South Africa -- Eastern Cape Agriculture -- Economic aspects -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/1774 , vital:27557
- Description: Irrigation farming is one of the most important rural development investments that can have both direct and indirect impacts on poverty and food security. The irregular and unreliable rainfall in the Eastern Cape Province has caused the smallholder farmers to deepen the irrigation farming systems as a strategy to cope with food shortage, income and poverty. South Africa has invested substantially in smallholder irrigation to benefit smallholder farmers in the less developed areas. Although the investment on small scale irrigation was to improve the welfare of the people, the performance and welfare impact of smallholder irrigation schemes has been poor and fall far short of the expectations of many stakeholders. The broad objective of the study was to examine the contribution of smallholder irrigation scheme on rural livelihoods of smallholder farmers at the project area. The null hypothesis was that smallholder irrigation schemes have no impact on farmer’s livelihoods. The alternative hypothesis for this study was that Melani irrigation scheme is contributing in improving rural livelihoods of smallholder farmers in the area. Cross study research design was used in the methodology. The purposive sampling was applied in order to choose a sample of 107 respondents that were interviewed using questionnaires, including both irrigating and non-irrigating farmers. A multiple linear regression analysis was used to find out whether the performance of the two groups of farmers is different and to what extent. To determine the specific role of irrigation an Independent T-Test was ran. Out of the selected sample 68 percent of households were farmers, 7.5 percent informal employed and 27 percent unemployed. It was found that the majority of participants were males and were uneducated. They depended on social grants for a living. The findings from the study showed that 84 percent of the respondents agreed that there is no market available in the village and farmers had limited access to credits. The crop income was the most contributing factor to household income. The results showed that whether the farmer is a non-irrigator or an irrigator the performance is not significant. Irrigation scheme improvement attained by smallholder farmers need to be maintained.
- Full Text:
In vitro evaluation of antimicrobial and antioxidant activities of olea europaea subsp. africana and euryops brevipapposus used by Cala community folkloric medicine for the management of infections associated with chronic non-communicable diseases
- Authors: Adegborioye, Abiodun
- Date: 2016
- Subjects: Antioxidants , Medicinal plants , Traditional medicine -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/4869 , vital:28624
- Description: Chronic non-communicable diseses are a global public health challenge that continuously threatens the development and health of humans. Risk factors such as unbalanced diet-the high consumption of processed food or food from animal origin are responsible for NCDs. NCDs result in weakened immune system, making the host susceptible to opportunistic infections. Thus, the NCDs burden is most times chronic and multiple with the illness and suffering of the affected person numerous. The lack of cure for NCDs, the high cost of drugs, their high side-effects, and the emergence of multiple drug resistance has given rise to the investigation of other sources for therapeutic cure such as medicinal plants. The ethanol, n-hexane and ethyl acetate extracts of Olea europaea were analysed for their antioxidant and antimicrobial activities. The essential oil was also analysed for their chemical constituents. The n-hexane extracts of O. europaea exhibited no inhibition against all of the microorganisms tested, while the ethyl acetate and ethanol extracts exhibited inhibition, with minimum inhibitory concentration values between 0.625 mg/ml to 1.25 mg/ml. The ethanol leaf and ethyl acetate stem extracts exhibited significant activity in the inhibition of 2, 2-azinobis-(3-ethylbenzothiazolin - 6-sulfonic acid diammonium salt (ABTS) free radical, the n-hexane leaf extract had the overall significant lipid peroxidation inhibition activity, while in the inhibition of 2, 2- diphenyl-1-picrylhydrazyl radical (DPPH), the ethanol and ethyl acetate leaf extracts had strong activity. Nonanal, phytol, α-Pinene, α-Phellandrene, spatulenol and farnesol were some of chemical components identified after the GC-MS analysis of O. europaea oil. In the final part of the dissertation, Euryops brevipapposus essential oil was assessed for the antioxidant activities using free radical scavenging assays. In addition to this, the antimicrobial activities were assessed and the chemical composition was analysed using GC-MS. The essential oil demonstrated significant antioxidant activity against 2, 2-diphenyl-2-picryl-hydrazyl free radical (DPPH), 2, 2′-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) and lipid peroxides with IC50 value of 0.0000000671 mg/ml, 1.05 mg/ml, and 1.170 mg/ml respectively. The essential oil also showed significant activity against all microorganisms tested with minimum inhibitory concentration (MIC) values between 0.055 mg/ml to 0.5 mg/ml. α-pinene, α- Phellandrene, germacrene D, β-pinene, trans- β.-Ocimene, bicyclogermacrene and β -Phellandrene were some of the chemical compounds identified in E. brevipapposus oil. The study has shown that E. brevipapposus and O. europaea are abundant in phytochemical compounds which were thought to be the root cause for the activities demonstrated. Therefore, these therapeutic properties observed validate and elucidate the traditional usage of the both plants in the treatment /management of diseases.
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- Authors: Adegborioye, Abiodun
- Date: 2016
- Subjects: Antioxidants , Medicinal plants , Traditional medicine -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/4869 , vital:28624
- Description: Chronic non-communicable diseses are a global public health challenge that continuously threatens the development and health of humans. Risk factors such as unbalanced diet-the high consumption of processed food or food from animal origin are responsible for NCDs. NCDs result in weakened immune system, making the host susceptible to opportunistic infections. Thus, the NCDs burden is most times chronic and multiple with the illness and suffering of the affected person numerous. The lack of cure for NCDs, the high cost of drugs, their high side-effects, and the emergence of multiple drug resistance has given rise to the investigation of other sources for therapeutic cure such as medicinal plants. The ethanol, n-hexane and ethyl acetate extracts of Olea europaea were analysed for their antioxidant and antimicrobial activities. The essential oil was also analysed for their chemical constituents. The n-hexane extracts of O. europaea exhibited no inhibition against all of the microorganisms tested, while the ethyl acetate and ethanol extracts exhibited inhibition, with minimum inhibitory concentration values between 0.625 mg/ml to 1.25 mg/ml. The ethanol leaf and ethyl acetate stem extracts exhibited significant activity in the inhibition of 2, 2-azinobis-(3-ethylbenzothiazolin - 6-sulfonic acid diammonium salt (ABTS) free radical, the n-hexane leaf extract had the overall significant lipid peroxidation inhibition activity, while in the inhibition of 2, 2- diphenyl-1-picrylhydrazyl radical (DPPH), the ethanol and ethyl acetate leaf extracts had strong activity. Nonanal, phytol, α-Pinene, α-Phellandrene, spatulenol and farnesol were some of chemical components identified after the GC-MS analysis of O. europaea oil. In the final part of the dissertation, Euryops brevipapposus essential oil was assessed for the antioxidant activities using free radical scavenging assays. In addition to this, the antimicrobial activities were assessed and the chemical composition was analysed using GC-MS. The essential oil demonstrated significant antioxidant activity against 2, 2-diphenyl-2-picryl-hydrazyl free radical (DPPH), 2, 2′-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) and lipid peroxides with IC50 value of 0.0000000671 mg/ml, 1.05 mg/ml, and 1.170 mg/ml respectively. The essential oil also showed significant activity against all microorganisms tested with minimum inhibitory concentration (MIC) values between 0.055 mg/ml to 0.5 mg/ml. α-pinene, α- Phellandrene, germacrene D, β-pinene, trans- β.-Ocimene, bicyclogermacrene and β -Phellandrene were some of the chemical compounds identified in E. brevipapposus oil. The study has shown that E. brevipapposus and O. europaea are abundant in phytochemical compounds which were thought to be the root cause for the activities demonstrated. Therefore, these therapeutic properties observed validate and elucidate the traditional usage of the both plants in the treatment /management of diseases.
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Incidence and molecular characterization of Hepatitis E virus from swine herds in the Eastern Cape province, South Africa
- Authors: Adelabu, Olusesan Adeyemi
- Date: 2016
- Subjects: Hepatitis -- South Africa -- Eastern Cape Virology -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/13966 , vital:39734
- Description: Hepatitis E virus-mediated infection is a serious public health concern in economically developing nations of the world. Globally, four major genotypes of HEV have been documented and the swine HEV identified to date in different countries belongs to either genotype 3 or 4. It has been reported to cross species barrier and cause infections in humans. Hence, Hepatitis E has been suggested to be zoonotic owing to the increase of evidence through various studies. Thus far, genotypes 1 and 2 are exclusively found in human population. However, little data are available regarding the occurrence of HEV in South Africa, especially in the Eastern Cape Province. Hence, this study was designed to assess the prevalence of Hepatitis E Virus among swine herd in selected communal and commercial farms in the Eastern Cape Province of South Africa. A total of 160 faecal samples were collected from swine herds in Amathole and Chris Hani District Municipalities of Eastern Cape Province and screened for the presence of HEV. Faecal samples were collected, using sterile swabs, from swine between the age of 2 to 6 months old, placed on ice and transported to the laboratory for further analysis. Viral RNA extraction was carried out followed by nested reverse-transcriptase polymerase chain reaction was used for amplification, after which the amplified products were examined by electrophoresis and viewed under ultraviolet illumination. The positive PCR products were sent for sequencing. Of the 160 faecal samples screened, only seven were positive (4.4percent) for HEV. The nucleotide sequence analyses revealed isolates UFT 01-03 sharing 82percent to 99percent identities with other strains with accession numbers KX896664, KX896665 and KX896666 and UMCF 01-04 with KX896667, KX896668, KX896669 and KX896670 from different regions of the world Phylogenetic analysis also showed that all seven isolates from this study clustered with both human and swine HEV from different geographical regions of the world especially with Japan Human and swine strains, Netherland swine HEV strains (AY032758, AF332620) and human HEV strain from France (JQ763611). We conclude that HEV is present among swine in the Eastern Cape Province, albeit in low incidence, and this does have public health implications. There is need for maintenance of high hygienic standards in order to prevent human infections through swine faecal materials and appropriate cooking of pork is highly advised.
- Full Text:
- Authors: Adelabu, Olusesan Adeyemi
- Date: 2016
- Subjects: Hepatitis -- South Africa -- Eastern Cape Virology -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/13966 , vital:39734
- Description: Hepatitis E virus-mediated infection is a serious public health concern in economically developing nations of the world. Globally, four major genotypes of HEV have been documented and the swine HEV identified to date in different countries belongs to either genotype 3 or 4. It has been reported to cross species barrier and cause infections in humans. Hence, Hepatitis E has been suggested to be zoonotic owing to the increase of evidence through various studies. Thus far, genotypes 1 and 2 are exclusively found in human population. However, little data are available regarding the occurrence of HEV in South Africa, especially in the Eastern Cape Province. Hence, this study was designed to assess the prevalence of Hepatitis E Virus among swine herd in selected communal and commercial farms in the Eastern Cape Province of South Africa. A total of 160 faecal samples were collected from swine herds in Amathole and Chris Hani District Municipalities of Eastern Cape Province and screened for the presence of HEV. Faecal samples were collected, using sterile swabs, from swine between the age of 2 to 6 months old, placed on ice and transported to the laboratory for further analysis. Viral RNA extraction was carried out followed by nested reverse-transcriptase polymerase chain reaction was used for amplification, after which the amplified products were examined by electrophoresis and viewed under ultraviolet illumination. The positive PCR products were sent for sequencing. Of the 160 faecal samples screened, only seven were positive (4.4percent) for HEV. The nucleotide sequence analyses revealed isolates UFT 01-03 sharing 82percent to 99percent identities with other strains with accession numbers KX896664, KX896665 and KX896666 and UMCF 01-04 with KX896667, KX896668, KX896669 and KX896670 from different regions of the world Phylogenetic analysis also showed that all seven isolates from this study clustered with both human and swine HEV from different geographical regions of the world especially with Japan Human and swine strains, Netherland swine HEV strains (AY032758, AF332620) and human HEV strain from France (JQ763611). We conclude that HEV is present among swine in the Eastern Cape Province, albeit in low incidence, and this does have public health implications. There is need for maintenance of high hygienic standards in order to prevent human infections through swine faecal materials and appropriate cooking of pork is highly advised.
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