Evaluation of cellulase and xylanase production by two actinobacteria species belonging to the Micrococcus genus isolated from decaying lignocellulosic biomass
- Mmango-Kaseke, Ziyanda https://orcid.org/0000-0002-8936-1149
- Authors: Mmango-Kaseke, Ziyanda https://orcid.org/0000-0002-8936-1149
- Date: 2016-05
- Subjects: Lignocellulose , Biomass energy
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/24197 , vital:62442
- Description: Bacteria were isolated from sawdust and screened for cellulase and xylanase production on carboxyl methyl cellulose (CMC) and birchwood xylan agar. The bacteria showing halo forms around the colony were selected for further analyses and those isolates with the highest cumulative halozone size (isolate PLY1 and MLY10) were chosen for detailed studies. Evaluation of cellulase and xylanase production by saw dust actinobacterial species whose 16S rDNA nucleotide sequences were deposited in GenBank as Micrococcus luteus strain SAMRC-UFH3 with accession number KU171371 and Micrococcus yunnanensis strain SAMRC-UFH4 with accession number KU171372. Optimum culture conditions for the production of cellulase for respective axenic culture include incubation period (96 h), incubation temperature (25oC), agitation speed (50 rpm), and pH 5. For xylanase production, the optimum culture conditions in the presence of 1percent (w/v) birchwood xylan include incubation period (84 h), incubation temperature (25oC), agitation speed (200 rpm), and pH 10. For Micrococcus yunnanensis strain SAMRC-UFH4 cellulase production was optimal under such conditions as, incubation temperature (30oC), agitation speed (0 rpm), and pH 5, while xylanase production was optimal at, incubation temperature (30oC), agitation speed (150 rpm), and pH 10. The high cellulase and xylanase activity obtained from these isolates suggest suitability of the organisms as important candidates for commercial application. , Thesis (MSc) -- Faculty of Science and Agriculture, 2016
- Full Text:
- Authors: Mmango-Kaseke, Ziyanda https://orcid.org/0000-0002-8936-1149
- Date: 2016-05
- Subjects: Lignocellulose , Biomass energy
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/24197 , vital:62442
- Description: Bacteria were isolated from sawdust and screened for cellulase and xylanase production on carboxyl methyl cellulose (CMC) and birchwood xylan agar. The bacteria showing halo forms around the colony were selected for further analyses and those isolates with the highest cumulative halozone size (isolate PLY1 and MLY10) were chosen for detailed studies. Evaluation of cellulase and xylanase production by saw dust actinobacterial species whose 16S rDNA nucleotide sequences were deposited in GenBank as Micrococcus luteus strain SAMRC-UFH3 with accession number KU171371 and Micrococcus yunnanensis strain SAMRC-UFH4 with accession number KU171372. Optimum culture conditions for the production of cellulase for respective axenic culture include incubation period (96 h), incubation temperature (25oC), agitation speed (50 rpm), and pH 5. For xylanase production, the optimum culture conditions in the presence of 1percent (w/v) birchwood xylan include incubation period (84 h), incubation temperature (25oC), agitation speed (200 rpm), and pH 10. For Micrococcus yunnanensis strain SAMRC-UFH4 cellulase production was optimal under such conditions as, incubation temperature (30oC), agitation speed (0 rpm), and pH 5, while xylanase production was optimal at, incubation temperature (30oC), agitation speed (150 rpm), and pH 10. The high cellulase and xylanase activity obtained from these isolates suggest suitability of the organisms as important candidates for commercial application. , Thesis (MSc) -- Faculty of Science and Agriculture, 2016
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Synthesis of bioethanol from lignocellulosic materials: A focus on grass and waste paper as raw materials
- Authors: Vala, Mavula Kikwe
- Date: 2009-12
- Subjects: Ethanol as fuel , Biomass energy , Lignocellulose -- Biotechnology
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/24499 , vital:63049
- Description: Biofuels are currently recognized as not only a necessity, but an inevitable pathway to secure the planet future energy needs. Food crops have been used (so far) as the biomass for bioethanol and biodiesel production. This has increased concerns over food security and led to the search for diversification and alternative feedstocks for biofuel production. The use of lignocellulosic materials, the most abundant, low cost and easy feedstock to harvest for bioethanol purpose, involves challenging production processes. Several approaches have been used to facilitate the breakdown of the biopolymer structure to produce fermentable sugars that can be converted to ethanol. Most of the approaches have used high temperatures and pressures and have often led to the production of inhibitors of fermentation. In this study, lignocellulosic materials from grass and newsprint were investigated as sources of biomass for bioethanol production using a chemical route (sulfuric acid hydrolysis) which made use of temperatures below 100°C at normal atmospheric pressure. Fermentation of toxic lignocellulosic hydrolyzates was possible after the development of a method for inhibitors removal. The method used treated wood chips as a stationary phase in a chromatographic column to remove inhibitors. This method is expected to be extended to applications such as in municipal wastewater treatment. Sugar yields of 22.26 and 8.9 g/L of hydrolyzate; and an ethanol yield of 184.5 and 130.4 mg/mL of must were achieved for 5g grass and newsprint respectively using optimum conditions of 2percent H2SO4 at 97.5°C for grass and 0.5percent H2SO4 at 97.5°C for newsprint during the hydrolysis process. Pure cellulose was used as a control for the biomass where 254.1 g/L of fermentable sugars were recovered from soluble cellulose and the yield of ethanol was 201.8 mg/mL. , Thesis (MSc) -- Faculty of Science and Agriculture, 2009
- Full Text:
- Authors: Vala, Mavula Kikwe
- Date: 2009-12
- Subjects: Ethanol as fuel , Biomass energy , Lignocellulose -- Biotechnology
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/24499 , vital:63049
- Description: Biofuels are currently recognized as not only a necessity, but an inevitable pathway to secure the planet future energy needs. Food crops have been used (so far) as the biomass for bioethanol and biodiesel production. This has increased concerns over food security and led to the search for diversification and alternative feedstocks for biofuel production. The use of lignocellulosic materials, the most abundant, low cost and easy feedstock to harvest for bioethanol purpose, involves challenging production processes. Several approaches have been used to facilitate the breakdown of the biopolymer structure to produce fermentable sugars that can be converted to ethanol. Most of the approaches have used high temperatures and pressures and have often led to the production of inhibitors of fermentation. In this study, lignocellulosic materials from grass and newsprint were investigated as sources of biomass for bioethanol production using a chemical route (sulfuric acid hydrolysis) which made use of temperatures below 100°C at normal atmospheric pressure. Fermentation of toxic lignocellulosic hydrolyzates was possible after the development of a method for inhibitors removal. The method used treated wood chips as a stationary phase in a chromatographic column to remove inhibitors. This method is expected to be extended to applications such as in municipal wastewater treatment. Sugar yields of 22.26 and 8.9 g/L of hydrolyzate; and an ethanol yield of 184.5 and 130.4 mg/mL of must were achieved for 5g grass and newsprint respectively using optimum conditions of 2percent H2SO4 at 97.5°C for grass and 0.5percent H2SO4 at 97.5°C for newsprint during the hydrolysis process. Pure cellulose was used as a control for the biomass where 254.1 g/L of fermentable sugars were recovered from soluble cellulose and the yield of ethanol was 201.8 mg/mL. , Thesis (MSc) -- Faculty of Science and Agriculture, 2009
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