Physiochemical, fatty acids, lipid oxidation, sensory characteristics and consumer acceptance of warthog cabanossi produced with pork backfat and fat-tailed sheep backfat
- Authors: Mahachi, Leo Nyikadzino
- Date: 2017
- Subjects: Fatty acids Lipids -- Oxidation
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/6259 , vital:29530
- Description: The objective of this study was to determine the effect of different fat inclusion levels and fat types on the physical and chemical attributes, lipid oxidation, fatty acid composition and sensory characteristics of warthog cabanossi. To achieve this, three types of cabanossi with different pork backfat levels (10 percent, 20 percent and 30 percent) were produced for the first experiment. The results from the study showed that different inclusion levels of pork backfat had an influence (P ≤ 0.05) on the physicochemical and fatty acid composition of warthog cabanossi but did not influence lipid oxidation (P > 0.05). The highest (P ≤0.05) pH, weight and moisture decline was observed in the 10 percent pork backfat cabanossi compared to the 20 percent and 30 percent treatments. However, no differences (P > 0.05) in the water activity of the product were observed. As expected total fat was lower in the 10 percent fat treatment and increased concomitantly. Similarly, protein, ash and salt were higher in the 10 percent fat cabanossi and decreased concomitantly. Differences in the fatty acid composition were observed between treatments. Furthermore, backfat level affected the sensory attributes and consumer acceptance of the cabanossi. Ten percent backfat cabanossi was scored higher (P ≤0.05) for most sensory attributes. Consequently, it was observed that the consumer panel preferred and scored the 10 percent fat cabanossi higher with regards to appearance and taste. In the second experiment, two cabanossi treatments of different fat types (pork backfat and fat-tailed sheep backfat) were produced. The weight loss, moisture content, pH, water activity and salt content did not differ (P > 0.05) between the two cabanossi products. However, there were differences (P ≤0.05) in the protein, fat and ash contents; where protein and ash were higher in the pork backfat cabanossi whilst fat was higher in the sheep backfat cabanossi. Thiobarbituric reactive substances (TBARS) were similar (P > 0.05) between the two fat types cabanossi which could be explained by similar fatty acid profiles being reported for the two cabanossi although the n-6:n-3 ratio was higher (P ≤0.05) in sheep backfat cabanossi. Results from the descriptive sensory analysis showed two distinct products (P ≤0.01) where pork backfat cabanossi scored higher for most attributes. However, the lower scores for sheep backfat cabanossi were within an acceptable range. Sheep backfat cabanossi were also scored for unique attributes that were not detected in the pork backfat cabanossi. This study concluded that fat-tailed sheep backfat can be used to produce an unique cabanossi product of acceptable quality.
- Full Text:
- Authors: Mahachi, Leo Nyikadzino
- Date: 2017
- Subjects: Fatty acids Lipids -- Oxidation
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/6259 , vital:29530
- Description: The objective of this study was to determine the effect of different fat inclusion levels and fat types on the physical and chemical attributes, lipid oxidation, fatty acid composition and sensory characteristics of warthog cabanossi. To achieve this, three types of cabanossi with different pork backfat levels (10 percent, 20 percent and 30 percent) were produced for the first experiment. The results from the study showed that different inclusion levels of pork backfat had an influence (P ≤ 0.05) on the physicochemical and fatty acid composition of warthog cabanossi but did not influence lipid oxidation (P > 0.05). The highest (P ≤0.05) pH, weight and moisture decline was observed in the 10 percent pork backfat cabanossi compared to the 20 percent and 30 percent treatments. However, no differences (P > 0.05) in the water activity of the product were observed. As expected total fat was lower in the 10 percent fat treatment and increased concomitantly. Similarly, protein, ash and salt were higher in the 10 percent fat cabanossi and decreased concomitantly. Differences in the fatty acid composition were observed between treatments. Furthermore, backfat level affected the sensory attributes and consumer acceptance of the cabanossi. Ten percent backfat cabanossi was scored higher (P ≤0.05) for most sensory attributes. Consequently, it was observed that the consumer panel preferred and scored the 10 percent fat cabanossi higher with regards to appearance and taste. In the second experiment, two cabanossi treatments of different fat types (pork backfat and fat-tailed sheep backfat) were produced. The weight loss, moisture content, pH, water activity and salt content did not differ (P > 0.05) between the two cabanossi products. However, there were differences (P ≤0.05) in the protein, fat and ash contents; where protein and ash were higher in the pork backfat cabanossi whilst fat was higher in the sheep backfat cabanossi. Thiobarbituric reactive substances (TBARS) were similar (P > 0.05) between the two fat types cabanossi which could be explained by similar fatty acid profiles being reported for the two cabanossi although the n-6:n-3 ratio was higher (P ≤0.05) in sheep backfat cabanossi. Results from the descriptive sensory analysis showed two distinct products (P ≤0.01) where pork backfat cabanossi scored higher for most attributes. However, the lower scores for sheep backfat cabanossi were within an acceptable range. Sheep backfat cabanossi were also scored for unique attributes that were not detected in the pork backfat cabanossi. This study concluded that fat-tailed sheep backfat can be used to produce an unique cabanossi product of acceptable quality.
- Full Text:
Prevalence of Group B streptococcus and staphylococcus aureus colonization in the anogenital tract of pregnant women in the Eastern Cape Province, South Africa
- Authors: Stofile, P Z
- Date: 2017
- Subjects: Streptococcal infections Staphylococcus aureus
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/5983 , vital:29462
- Description: Neonatal sickness and death is increasingly becoming a public health problem worldwide. The colonization of Group B Streptococcus and Staphylococcus in the rectovaginal area is among the sources of infections in neonates which can result in illness and mortality. The over exposure of humans to antibiotics is the possible cause of resistance in bacteria. These resistant strains can be passed onto offspring, leading to resistant infections and increasing the morbidity of neonates because of treatment failures. Many people, including healthcare personnel are not aware of the effect of these bacteria, and informing clinics and hospitals can help create awareness and monitoring the levels of resistance among bacteria can assist in preventing the transference of the bacteria. In this study we investigated the prevalence of group B Streptococcus (GBS) and Staphylococcus aureus in the anogenital tract of pregnant women in the Eastern Cape Province, South Africa. A total of 49 isolates from 25 (30.5 percent) pregnant women colonized with GBS were isolated from vaginal and rectal swabs of 82 pregnant women at 25-37 gestation who participated in this study. These isolates were obtained using standard microbiological methods and confirmed by polymerase chain reaction (PCR) technique aimed at the ScpB gene. The isolates were further screened for the presence of 9 serogroups (Ia, Ib, II, III, IV, V, VI, VII, VII) and serogroups Ib 2 (4.8 percent), II 20 (40.8 percent) and IV 5 (10.2 percent) and 22 non-typable (44.9 percent) were identified. Susceptibility profiling of the isolates to 12 antibiotics (tetracycline, clindamycin, erythromycin, gentamycin, naladixic acid, norfloxacin, chloramphenicol, cefuroxime, cefotaxime, imipenem, penicillin and vancomycin) was tested in vitro by the standardized disc diffusion method. All the confirmed GBS isolates (49) were resistant to erythromycin, tetracycline and clindamycin. A higher percentage of the isolates were resistant to gentamycin 44 (90 percent), nalidixic acid 41 (84 percent), penicillin 41 (84 percent), chloramphenicol 38 (78 percent), cefuroxime 36 (74 percent), imipenem 36 (74 percent), cefotaxime 35 (71 percent), norfloxacin 32 (65 percent) and vancomycin 31 (78 percent). Multiple antimicrobial resistance patterns ranged from 9‒11 and indices ranged from 0.7‒0.9, respectively. Among the antimicrobial resistance determinants examined, genes encoding for resistance to erythromycin ermB 25 (51 percent), tetracycline tetM 32 (65 percent) and penicillin bla-Z 4 (8 percent) only were identified. On the other hand, screening for S. aureus yielded a total of 7 isolates from 4 study participants as confirmed by PCR based on staphylococcal, nuc gene. The isolates were further screened for the presence of six virulence genes (Hla, Hlb, LUKM, LUKED, PVL, Eta and Etb) and antibiotic susceptibility pattern by the disc diffusion method using 12 (penicillin, vancomycin, tetracycline, rifampicin, imipenem, gentamycin, chloramphenicol, norfloxacin, oxacillin, erythromycin and sulfamethoxazole-trimethoprim) antibiotics that are adopted in the treatment of infections caused by the organism. PVL 6 (85.7 percent) and eta 1 (14.3 percent) were the two virulence genes detected. The following percentages of antibiotics resistance among the isolates were observed; penicillin G 7 (100 percent), clindamycin 7 (100 percent), vancomycin 5 (100 percent), rifampicin 5 (71 percent), oxacillin 5 (71 percent), erythromycin 5 (71 percent) gentamycin 3 (43 percent), norfloxacin 3 (43 percent), sulfamethoxazole-trimethoprim 3 (43 percent), chloramphenicol 2 (29 percent), imipenem 1 (14 percent). Multiple antimicrobial resistance patterns ranged from 7‒8 and indices ranged from 0.6‒0.7, respectively. Genetic profiling of the resistance genes identified erythromycin ermB 5(71.4 percent), tetracycline tetM 5(71.4 percent) and penicillin bla-Z 1(14.3 percent) only. The findings from the study have revealed GBS and S. aureus colonization of pregnant women in the Eastern Cape Province, and these have great public health implications especially for the neonates who are mostly likely to be infected during birth. The unidentifiable multidrug resistant serogroups of GBS as well as resistant S. aureus limit the choice of drugs in the management of infections caused by these pathogens more so if transmitted to infants. Therefore asymptomatic pregnant women needed to be properly educated about the bacteria as well as the precautions that need to be taken.
- Full Text:
- Authors: Stofile, P Z
- Date: 2017
- Subjects: Streptococcal infections Staphylococcus aureus
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/5983 , vital:29462
- Description: Neonatal sickness and death is increasingly becoming a public health problem worldwide. The colonization of Group B Streptococcus and Staphylococcus in the rectovaginal area is among the sources of infections in neonates which can result in illness and mortality. The over exposure of humans to antibiotics is the possible cause of resistance in bacteria. These resistant strains can be passed onto offspring, leading to resistant infections and increasing the morbidity of neonates because of treatment failures. Many people, including healthcare personnel are not aware of the effect of these bacteria, and informing clinics and hospitals can help create awareness and monitoring the levels of resistance among bacteria can assist in preventing the transference of the bacteria. In this study we investigated the prevalence of group B Streptococcus (GBS) and Staphylococcus aureus in the anogenital tract of pregnant women in the Eastern Cape Province, South Africa. A total of 49 isolates from 25 (30.5 percent) pregnant women colonized with GBS were isolated from vaginal and rectal swabs of 82 pregnant women at 25-37 gestation who participated in this study. These isolates were obtained using standard microbiological methods and confirmed by polymerase chain reaction (PCR) technique aimed at the ScpB gene. The isolates were further screened for the presence of 9 serogroups (Ia, Ib, II, III, IV, V, VI, VII, VII) and serogroups Ib 2 (4.8 percent), II 20 (40.8 percent) and IV 5 (10.2 percent) and 22 non-typable (44.9 percent) were identified. Susceptibility profiling of the isolates to 12 antibiotics (tetracycline, clindamycin, erythromycin, gentamycin, naladixic acid, norfloxacin, chloramphenicol, cefuroxime, cefotaxime, imipenem, penicillin and vancomycin) was tested in vitro by the standardized disc diffusion method. All the confirmed GBS isolates (49) were resistant to erythromycin, tetracycline and clindamycin. A higher percentage of the isolates were resistant to gentamycin 44 (90 percent), nalidixic acid 41 (84 percent), penicillin 41 (84 percent), chloramphenicol 38 (78 percent), cefuroxime 36 (74 percent), imipenem 36 (74 percent), cefotaxime 35 (71 percent), norfloxacin 32 (65 percent) and vancomycin 31 (78 percent). Multiple antimicrobial resistance patterns ranged from 9‒11 and indices ranged from 0.7‒0.9, respectively. Among the antimicrobial resistance determinants examined, genes encoding for resistance to erythromycin ermB 25 (51 percent), tetracycline tetM 32 (65 percent) and penicillin bla-Z 4 (8 percent) only were identified. On the other hand, screening for S. aureus yielded a total of 7 isolates from 4 study participants as confirmed by PCR based on staphylococcal, nuc gene. The isolates were further screened for the presence of six virulence genes (Hla, Hlb, LUKM, LUKED, PVL, Eta and Etb) and antibiotic susceptibility pattern by the disc diffusion method using 12 (penicillin, vancomycin, tetracycline, rifampicin, imipenem, gentamycin, chloramphenicol, norfloxacin, oxacillin, erythromycin and sulfamethoxazole-trimethoprim) antibiotics that are adopted in the treatment of infections caused by the organism. PVL 6 (85.7 percent) and eta 1 (14.3 percent) were the two virulence genes detected. The following percentages of antibiotics resistance among the isolates were observed; penicillin G 7 (100 percent), clindamycin 7 (100 percent), vancomycin 5 (100 percent), rifampicin 5 (71 percent), oxacillin 5 (71 percent), erythromycin 5 (71 percent) gentamycin 3 (43 percent), norfloxacin 3 (43 percent), sulfamethoxazole-trimethoprim 3 (43 percent), chloramphenicol 2 (29 percent), imipenem 1 (14 percent). Multiple antimicrobial resistance patterns ranged from 7‒8 and indices ranged from 0.6‒0.7, respectively. Genetic profiling of the resistance genes identified erythromycin ermB 5(71.4 percent), tetracycline tetM 5(71.4 percent) and penicillin bla-Z 1(14.3 percent) only. The findings from the study have revealed GBS and S. aureus colonization of pregnant women in the Eastern Cape Province, and these have great public health implications especially for the neonates who are mostly likely to be infected during birth. The unidentifiable multidrug resistant serogroups of GBS as well as resistant S. aureus limit the choice of drugs in the management of infections caused by these pathogens more so if transmitted to infants. Therefore asymptomatic pregnant women needed to be properly educated about the bacteria as well as the precautions that need to be taken.
- Full Text:
Quality indices of the Eastern Cape beach waters in Buffalo City Metropolitan Municipality, Eastern Cape, South Africa
- Authors: Mani, Sinalo
- Date: 2017
- Subjects: Waterfronts -- Recreational use -- South Africa -- Eastern Cape Water quality -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/19054 , vital:40108
- Description: Surface waters constitute important sources of water and serve for agricultural, recreational and industrial purposes; however, the vulnerability of such water sources to pollution presents serious ecological, public health and economic problems of global concerns. Beach water quality is monitored to reduce the risk of recreational waterborne illnesses. Water samples were collected between September 2014 and August 2015 at Eastern beach and a total of 5 physicochemical parameters, counts of faecal coliforms, E. coli and Enterococcus isolates were determined using standard analytical procedures. Confirmed Escherichia coli isolates were assessed for virulence genes associated with pathogenic E. coli strains responsible for intestinal and extra-intestinal infections. Recovered E. coli isolates were then interpreted for their antibiogram profiling using disk diffusion method. On the other hand, the recovered Enterococcus isolates were characterized for Enterococcus speciation of key species that are normally implicated in infections and further assessed for their antibiogram profiling by disk diffusion method. The physicochemical qualities of the water ranged as follows: turbidity (1.4 to 36.8 NTU); pH (7.0 to 8.6); temperature (14.5 to 24.5 ºC); total dissolved solids (TDS) (5.2 to 46 mg/L) and electrical conductivity (EC) (8.2 to 70.7 μS/cm). Microbiological qualities obtained were as follows: faecal coliform (101 to 103 CFU/100 mL); Escherichia coli (101 to 103 CFU/100 mL); and enterococci (101 CFU/100 mL to 102 CFU/100 mL). Only 38/104 (37 percent) harbored at least 1 virulence gene while 66/104 (63 percent) of the isolates harbored none. Most frequent virulence genes were eae for EPEC/EHEC, fliCH7 for EHEC, ibeA for NMEC, while daaE for DAEC and ipaH for EIEC were discovered in three isolates each; bfp for EPEC was detected in 2 isolates and lt gene for ETEC in one isolate. EAEC and UPEC genes were not detected in all the isolates. All tested isolates were resistant to ampicillin, tetracycline, gentamycin and chloramphenicol (85 percent), ciprofloxacin (78 percent), trimethoprim (54 percent) and norfloxacin (54 percent) and the Multiple Antibiotic Resistance Index for all isolates ranged between 0.6- 1.0 with the average being 0.8, which exceeded the threshold value of 0.2. Among the 96 recovered Enterococcus isolates, 55 percent were identified as E. faecium, 22 percent were E. hirae, 2 percent were E. durans and 21 percent were uncharacterized Enterococcus species. E. faecalis and E. casseliflavus were not detected amongst the isolates. All isolates were resistant to vancomycin, erythromycin and chloramphenicol. While the resistance against other antibiotics followed the order: tetracycline (97 percent), gentamycin (96 percent), ciprofloxacin (93 percent), linezolid (91 percent), ampicillin (91 percent) and trimethoprim (62percent). Only 6 percent of the isolates were susceptible to trimethoprim and the MAR index for all isolates ranged between 0.6- 1.0 with the average being 0.9 which greater than 0.2, suggesting that these bacterial strains originates from environments with high use or contamination of antibiotics.
- Full Text:
- Authors: Mani, Sinalo
- Date: 2017
- Subjects: Waterfronts -- Recreational use -- South Africa -- Eastern Cape Water quality -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/19054 , vital:40108
- Description: Surface waters constitute important sources of water and serve for agricultural, recreational and industrial purposes; however, the vulnerability of such water sources to pollution presents serious ecological, public health and economic problems of global concerns. Beach water quality is monitored to reduce the risk of recreational waterborne illnesses. Water samples were collected between September 2014 and August 2015 at Eastern beach and a total of 5 physicochemical parameters, counts of faecal coliforms, E. coli and Enterococcus isolates were determined using standard analytical procedures. Confirmed Escherichia coli isolates were assessed for virulence genes associated with pathogenic E. coli strains responsible for intestinal and extra-intestinal infections. Recovered E. coli isolates were then interpreted for their antibiogram profiling using disk diffusion method. On the other hand, the recovered Enterococcus isolates were characterized for Enterococcus speciation of key species that are normally implicated in infections and further assessed for their antibiogram profiling by disk diffusion method. The physicochemical qualities of the water ranged as follows: turbidity (1.4 to 36.8 NTU); pH (7.0 to 8.6); temperature (14.5 to 24.5 ºC); total dissolved solids (TDS) (5.2 to 46 mg/L) and electrical conductivity (EC) (8.2 to 70.7 μS/cm). Microbiological qualities obtained were as follows: faecal coliform (101 to 103 CFU/100 mL); Escherichia coli (101 to 103 CFU/100 mL); and enterococci (101 CFU/100 mL to 102 CFU/100 mL). Only 38/104 (37 percent) harbored at least 1 virulence gene while 66/104 (63 percent) of the isolates harbored none. Most frequent virulence genes were eae for EPEC/EHEC, fliCH7 for EHEC, ibeA for NMEC, while daaE for DAEC and ipaH for EIEC were discovered in three isolates each; bfp for EPEC was detected in 2 isolates and lt gene for ETEC in one isolate. EAEC and UPEC genes were not detected in all the isolates. All tested isolates were resistant to ampicillin, tetracycline, gentamycin and chloramphenicol (85 percent), ciprofloxacin (78 percent), trimethoprim (54 percent) and norfloxacin (54 percent) and the Multiple Antibiotic Resistance Index for all isolates ranged between 0.6- 1.0 with the average being 0.8, which exceeded the threshold value of 0.2. Among the 96 recovered Enterococcus isolates, 55 percent were identified as E. faecium, 22 percent were E. hirae, 2 percent were E. durans and 21 percent were uncharacterized Enterococcus species. E. faecalis and E. casseliflavus were not detected amongst the isolates. All isolates were resistant to vancomycin, erythromycin and chloramphenicol. While the resistance against other antibiotics followed the order: tetracycline (97 percent), gentamycin (96 percent), ciprofloxacin (93 percent), linezolid (91 percent), ampicillin (91 percent) and trimethoprim (62percent). Only 6 percent of the isolates were susceptible to trimethoprim and the MAR index for all isolates ranged between 0.6- 1.0 with the average being 0.9 which greater than 0.2, suggesting that these bacterial strains originates from environments with high use or contamination of antibiotics.
- Full Text:
Quality indices of the Nahoon beach and canal in Buffalo City Metropolitan Municipality, Eastern Cape, South Africa
- Authors: Ebomah, Kingsley Ehi
- Date: 2017
- Subjects: Pathogenic bacteria -- South Africa -- Eastern Cape Water quality -- South Africa -- Eastern Cape -- Measurement Water -- Microbiology -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/8122 , vital:31525
- Description: Faecal indicator bacteria have long been recommended for usage in water quality monitoring of various water sources including surface water. A large number of people in different parts of the world often engage in recreational and other commercial activities at the various beaches along the coastal lines, most especially, during summer season and festive periods. It is generally assumed that beach water is safe all the times, however, there have been increasing reports of beach and other coastal water pollutions because of rising anthropogenic activities around coastal areas globally. The presence of pathogens in faecal contaminated beach water either directly or indirectly increases the risk of human exposure which may result in skin infections as well as the ingestion and transmission of multidrug resistant pathogens. Microorganisms may gain entry into beach water directly from improperly treated final effluents released by wastewater treatment plants and from agricultural runoffs and/or faulty drainages. The aim of this study was to evaluate the quality indices of the Nahoon Beach and canal waters in the Eastern Cape Province of South Africa. In order to achieve this aim, the objectives of this study were as follow: to determine the seasonal variations of the physicochemical characteristics of the Nahoon beach and canal waters, to assess the microbiological quality of the beach water by assessing the prevalence of potentially E. coli and Enterococcus species, and to evaluate the antibiogram profile of the E. coli pathotypes and Enterococci species recovered from the beach water samples. Water samples were collected bi-weekly for a period of twelve months, during which on-site physicochemical analyses were carried out. Bacteriological assessment was performed using the membrane filtration technique and the necessary incubation conditions were followed. Identification of the target organisms was done by molecular based methods using PCR (polymerase chain reaction) while antibiotic susceptibility testing was done using the disk diffusion method. During the spring season (August to October) and periods of festive activities (December, January and March), it was observed that turbidity was at its highest, consequently, influencing total dissolved solids and a rise in the values of pH and electrical conductivity. Microbiological qualities assessed follow the order: Escherichia coli (101 – 103 CFU/100ml) and Enterococcus (101 – 102 CFU/100 ml). The results show that 41.2percent (107/260) of the presumptive E. coli isolates were positive for uidA gene. A total of 73 out of 107 (68.2percent) PCR-confirmed E. coli isolates were observed to belong to different pathotypes while 59percent (102/172) of the presumptive Enterococcus strains isolated were positive for SodA gene (Enterococcus spp.). Out of 102 enterococci isolates, 45 strains were identified as species (E. faecalis and E. faecium) and these were subjected to antibiotic susceptibility testing. The E. coli strains exhibited a frequency of 100percent resistance against ampicillin while the Enterococcus strains also showed 100percent resistance against vancomycin and linezolid. Target genes eae 11 (10.3percent); ipaH 14 (13.1percent); ibeA 48 (44.9percent) that encode pathogenicity in E. coli were successfully amplified by PCR suggesting that the isolates are potentially pathogenic strains whereas Enterococcus spp. including Enterococcus faecalis 26 (25.5percent) and Enterococcus faecium 19 (18.6percent) were also identified in the water samples. In this study, the phenotypic antibiogram profiles of the E. coli isolates revealed that all 73 (100percent) of the pathotypes were resistant to ampicillin, 67 (91.8percent) were resistant to amikacin, gentamicin and ceftazidime while about 61 (83.6percent) and 51 (69.9percent) were resistant to tetracycline and ciprofloxacin as well as trimethoprim respectively. About 21.9percent (16) of the E. coli isolates exhibited multiple antibiotic resistances with 100percent being resistant to eight antibiotics whereas multiple antimicrobial resistances were observed in 14 (31percent) of the enterococci strains and the most predominant profile was the simultaneous resistance to ampicillin, penicillin and vancomycin. The results obtained in this study show that the beach water generally did not comply with the set guidelines. The results also suggest the possible survival and prevalence of potentially virulent E. coli as well as Enterococcus strains in the aquatic environment which may be a cause of concern for public health. Monitoring of the quality of surface water and antimicrobial resistance (AMR) in bacteria is of great importance and has clinical and human health significance. Nonetheless, this study underscores the need for more detailed study of other waterborne pathogens of health importance including enteric viruses to ascertain the safety of the beach for human use.
- Full Text:
- Authors: Ebomah, Kingsley Ehi
- Date: 2017
- Subjects: Pathogenic bacteria -- South Africa -- Eastern Cape Water quality -- South Africa -- Eastern Cape -- Measurement Water -- Microbiology -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/8122 , vital:31525
- Description: Faecal indicator bacteria have long been recommended for usage in water quality monitoring of various water sources including surface water. A large number of people in different parts of the world often engage in recreational and other commercial activities at the various beaches along the coastal lines, most especially, during summer season and festive periods. It is generally assumed that beach water is safe all the times, however, there have been increasing reports of beach and other coastal water pollutions because of rising anthropogenic activities around coastal areas globally. The presence of pathogens in faecal contaminated beach water either directly or indirectly increases the risk of human exposure which may result in skin infections as well as the ingestion and transmission of multidrug resistant pathogens. Microorganisms may gain entry into beach water directly from improperly treated final effluents released by wastewater treatment plants and from agricultural runoffs and/or faulty drainages. The aim of this study was to evaluate the quality indices of the Nahoon Beach and canal waters in the Eastern Cape Province of South Africa. In order to achieve this aim, the objectives of this study were as follow: to determine the seasonal variations of the physicochemical characteristics of the Nahoon beach and canal waters, to assess the microbiological quality of the beach water by assessing the prevalence of potentially E. coli and Enterococcus species, and to evaluate the antibiogram profile of the E. coli pathotypes and Enterococci species recovered from the beach water samples. Water samples were collected bi-weekly for a period of twelve months, during which on-site physicochemical analyses were carried out. Bacteriological assessment was performed using the membrane filtration technique and the necessary incubation conditions were followed. Identification of the target organisms was done by molecular based methods using PCR (polymerase chain reaction) while antibiotic susceptibility testing was done using the disk diffusion method. During the spring season (August to October) and periods of festive activities (December, January and March), it was observed that turbidity was at its highest, consequently, influencing total dissolved solids and a rise in the values of pH and electrical conductivity. Microbiological qualities assessed follow the order: Escherichia coli (101 – 103 CFU/100ml) and Enterococcus (101 – 102 CFU/100 ml). The results show that 41.2percent (107/260) of the presumptive E. coli isolates were positive for uidA gene. A total of 73 out of 107 (68.2percent) PCR-confirmed E. coli isolates were observed to belong to different pathotypes while 59percent (102/172) of the presumptive Enterococcus strains isolated were positive for SodA gene (Enterococcus spp.). Out of 102 enterococci isolates, 45 strains were identified as species (E. faecalis and E. faecium) and these were subjected to antibiotic susceptibility testing. The E. coli strains exhibited a frequency of 100percent resistance against ampicillin while the Enterococcus strains also showed 100percent resistance against vancomycin and linezolid. Target genes eae 11 (10.3percent); ipaH 14 (13.1percent); ibeA 48 (44.9percent) that encode pathogenicity in E. coli were successfully amplified by PCR suggesting that the isolates are potentially pathogenic strains whereas Enterococcus spp. including Enterococcus faecalis 26 (25.5percent) and Enterococcus faecium 19 (18.6percent) were also identified in the water samples. In this study, the phenotypic antibiogram profiles of the E. coli isolates revealed that all 73 (100percent) of the pathotypes were resistant to ampicillin, 67 (91.8percent) were resistant to amikacin, gentamicin and ceftazidime while about 61 (83.6percent) and 51 (69.9percent) were resistant to tetracycline and ciprofloxacin as well as trimethoprim respectively. About 21.9percent (16) of the E. coli isolates exhibited multiple antibiotic resistances with 100percent being resistant to eight antibiotics whereas multiple antimicrobial resistances were observed in 14 (31percent) of the enterococci strains and the most predominant profile was the simultaneous resistance to ampicillin, penicillin and vancomycin. The results obtained in this study show that the beach water generally did not comply with the set guidelines. The results also suggest the possible survival and prevalence of potentially virulent E. coli as well as Enterococcus strains in the aquatic environment which may be a cause of concern for public health. Monitoring of the quality of surface water and antimicrobial resistance (AMR) in bacteria is of great importance and has clinical and human health significance. Nonetheless, this study underscores the need for more detailed study of other waterborne pathogens of health importance including enteric viruses to ascertain the safety of the beach for human use.
- Full Text:
Screening of shark liver extracts for potential antimicrobial properties against selected pathogenic bacterial strains
- Authors: Mrwetyana, Thandolwethu
- Date: 2017
- Subjects: Fishes -- Diseases Pathogenic bacteria Antibiotics
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/12982 , vital:39413
- Description: The growing problem of antimicrobial resistance prevents the effective treatment of bacterial infections. Traditional antibiotics such as penicillin have been rendered ineffective against most microbial pathogens. This has led to an increased need for the development of new and improved drugs. The marine environment contains a great array of organisms with unique biological properties, but still remains one of our most underutilized biological resources. The aim of this study was to screen different shark liver extracts for antimicrobial properties. After optimizing the extraction methods, the liver extracts (oil and aminosterol) of three different shark species, namely the Dogfish (Squalus acanthias), the Catshark (Scyliorhinus capensis) and the Hammerhead (Sphyrna zygaena) shark, were screened for antimicrobial properties using the Kirby-Bauer disc diffusion method against selected bacterial pathogens (Helicobacter pylori, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus cereus), after which the MIC was determined using the modified broth micro-dilution described by Vollekova et al. (2001). The most active extract was fractionated using thin layer chromatography, and TLC-direct bioautography was used to determine the antimicrobial properties of the fractionated compounds. The Folch et al and the Shinnar et al methods yielded the highest extract volumes for oil and aminosterol consecutively, and the catshark and dogfish aminosterol extract showed greater levels of bioactivity against all selected bacterial pathogens, with S. aureus showing highest susceptibility levels to both extracts. A total of 22 compounds were observed in the developed plates with two compounds (Rf 0.53 and 0.79) showing antimicrobial activity. Certain shark liver extracts possess antimicrobial properties that have the potential to be used in the development of new antimicrobial drugs.
- Full Text:
- Authors: Mrwetyana, Thandolwethu
- Date: 2017
- Subjects: Fishes -- Diseases Pathogenic bacteria Antibiotics
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/12982 , vital:39413
- Description: The growing problem of antimicrobial resistance prevents the effective treatment of bacterial infections. Traditional antibiotics such as penicillin have been rendered ineffective against most microbial pathogens. This has led to an increased need for the development of new and improved drugs. The marine environment contains a great array of organisms with unique biological properties, but still remains one of our most underutilized biological resources. The aim of this study was to screen different shark liver extracts for antimicrobial properties. After optimizing the extraction methods, the liver extracts (oil and aminosterol) of three different shark species, namely the Dogfish (Squalus acanthias), the Catshark (Scyliorhinus capensis) and the Hammerhead (Sphyrna zygaena) shark, were screened for antimicrobial properties using the Kirby-Bauer disc diffusion method against selected bacterial pathogens (Helicobacter pylori, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus cereus), after which the MIC was determined using the modified broth micro-dilution described by Vollekova et al. (2001). The most active extract was fractionated using thin layer chromatography, and TLC-direct bioautography was used to determine the antimicrobial properties of the fractionated compounds. The Folch et al and the Shinnar et al methods yielded the highest extract volumes for oil and aminosterol consecutively, and the catshark and dogfish aminosterol extract showed greater levels of bioactivity against all selected bacterial pathogens, with S. aureus showing highest susceptibility levels to both extracts. A total of 22 compounds were observed in the developed plates with two compounds (Rf 0.53 and 0.79) showing antimicrobial activity. Certain shark liver extracts possess antimicrobial properties that have the potential to be used in the development of new antimicrobial drugs.
- Full Text:
Small-scale farmers' participation in planning and implementation of farmer support programmes in Amahlathi Local Municipality, Eastern Cape Province, South Africa
- Authors: Makunga, Phendulwa Zikhona
- Date: 2017
- Subjects: Agricultural development projects , Farms, Small -- South Africa -- Eastern Cape , Agriculture -- Economic aspects -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/6248 , vital:29529
- Description: The democratic government in South Africa has brought about several policies which were initiated for rural development, and one of the government’s strategies was to involve the agricultural sector. Farmer Support Programmes (FSPs) were developed to assist small-scale farmers to become commercial farmers. The study looked at the participation of small scale farmers in the planning and implementation of the FSPs in the Amahlathi Local Municipality. 108 small scale farmers were selected using a non-random purposive probability and twelve farmers’ organisations. Semi-structured questionnaires and semi-structured interviews were used as the data collection tool and method respectively. The data analysis used was a descriptive analysis and a thematic analysis. The results revealed that the farmers have a positive perception towards the support programmes being helpful in their farming needs. The positive perception was especially on the extension and advisory services, dipping and vaccination programme, and the supply and funding of inputs and assets. However, the results revealed that there was no significant difference between gender and the perception of the farmers when a chi square test of association was performed. The results also revealed that the farmers and farmers’ organisations were not involved in the planning of the programmes by the government. It is therefore recommended that for the success of the FSP and for economic growth in the rural areas, the government has to avoid using a top-down approach when developing these programmes, and instead consult the farmers and the farmers’ organisations to know the specific needs of the farmers in their farming practices.
- Full Text:
- Authors: Makunga, Phendulwa Zikhona
- Date: 2017
- Subjects: Agricultural development projects , Farms, Small -- South Africa -- Eastern Cape , Agriculture -- Economic aspects -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/6248 , vital:29529
- Description: The democratic government in South Africa has brought about several policies which were initiated for rural development, and one of the government’s strategies was to involve the agricultural sector. Farmer Support Programmes (FSPs) were developed to assist small-scale farmers to become commercial farmers. The study looked at the participation of small scale farmers in the planning and implementation of the FSPs in the Amahlathi Local Municipality. 108 small scale farmers were selected using a non-random purposive probability and twelve farmers’ organisations. Semi-structured questionnaires and semi-structured interviews were used as the data collection tool and method respectively. The data analysis used was a descriptive analysis and a thematic analysis. The results revealed that the farmers have a positive perception towards the support programmes being helpful in their farming needs. The positive perception was especially on the extension and advisory services, dipping and vaccination programme, and the supply and funding of inputs and assets. However, the results revealed that there was no significant difference between gender and the perception of the farmers when a chi square test of association was performed. The results also revealed that the farmers and farmers’ organisations were not involved in the planning of the programmes by the government. It is therefore recommended that for the success of the FSP and for economic growth in the rural areas, the government has to avoid using a top-down approach when developing these programmes, and instead consult the farmers and the farmers’ organisations to know the specific needs of the farmers in their farming practices.
- Full Text:
Socio-economic benefits of agricultural projects to surrounding communities: the case of Qamata Irrigation Scheme in the Eastern Cape, South Africa
- Authors: Masela, Zandile
- Date: 2017
- Subjects: Agricultural development projects -- South Africa -- Eastern Cape , Agriculture -- Economic aspects -- South Africa -- Eastern Cape , Irrigation farming -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/4716 , vital:28501
- Description: The Qamata Irrigation Scheme (QIS) is one of the largest irrigation schemes in South Africa. Despite substantial state investment, community members have only derived limited benefits from the scheme, leading the government efforts to revitalize the scheme. Many studies have been conducted about QIS but none of them have analyzed the effects of the QIS on the surrounding communities. This study investigated the socio-economic benefits of QIS to surrounding communities. For the purposes of this study, the units of analysis were the surrounding household members, questionnaires were used to collect data. Probabilistic sampling of random sampling method was used to select the villages; respondents’ were availability selected with sample size of 197 households. Quantitative and qualitative data were collected from the participants. To analyse data, descriptive statistics and multinomial logit regression model were used. The study found out that female-headed households constitute 63.45 percent compared to male-headed households at 36.55 percent. The average age of the households in the villages that are within the radius of 10km is 58 years, while it is 59 years in villages that are beyond the 10km radius. In villages within the radius of 10km, 86.08 percent of respondents are unemployed, 5.70 percent are employed at the scheme and 8.23 percent are self-employed. In villages beyond 10km, 84.62 percent of respondents are unemployed, 5.13 percent are employed at the scheme and 10.26 percent are self-employed. In both villages, 71 percent of respondents reported that that they do not get feed supply from the scheme while 29 percent of respondents reported that they get feed supply from the scheme. From the overall distribution of villages within and beyond the radius of 10km, 80 percent of respondents reported that they do not get job opportunities from the QIS while 20 percent of respondents reported that they get job opportunities from the QIS. The study shows that 53 percent of respondents from villages within the radius of 10km are low-benefitting, 33 percent of respondents are intermediate-benefitting and 14 percent of respondents are high-benefitting from the scheme, while in villages beyond the radius of 10km, 97 percent of respondents are low-benefitting, 3 percent of respondents are intermediate-benefitting and none of the respondents is high-benefitting from the scheme. Furthermore, the study, through the multinomial logit regression model, shows that gender for intermediate benefitting has a negative coefficient (-1.70) and shows a significance of 10 percent On the other hand, gender for high benefitting has a positive coefficient (2.57) and shows a significance of 1 percent. Distance from the scheme has a negative coefficient (-3.08) and shows a significance of 1 percent. Access to farmland has a positive coefficient (2.33) and shows a significance of 10 percent. The study recommends that surrounding communities should own home gardens so that they can grow fresh produce and create job opportunities. The surrounding communities are also encouraged to own more farmlands or produce their own crops so they can also learn from the skills of the scheme.
- Full Text:
- Authors: Masela, Zandile
- Date: 2017
- Subjects: Agricultural development projects -- South Africa -- Eastern Cape , Agriculture -- Economic aspects -- South Africa -- Eastern Cape , Irrigation farming -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/4716 , vital:28501
- Description: The Qamata Irrigation Scheme (QIS) is one of the largest irrigation schemes in South Africa. Despite substantial state investment, community members have only derived limited benefits from the scheme, leading the government efforts to revitalize the scheme. Many studies have been conducted about QIS but none of them have analyzed the effects of the QIS on the surrounding communities. This study investigated the socio-economic benefits of QIS to surrounding communities. For the purposes of this study, the units of analysis were the surrounding household members, questionnaires were used to collect data. Probabilistic sampling of random sampling method was used to select the villages; respondents’ were availability selected with sample size of 197 households. Quantitative and qualitative data were collected from the participants. To analyse data, descriptive statistics and multinomial logit regression model were used. The study found out that female-headed households constitute 63.45 percent compared to male-headed households at 36.55 percent. The average age of the households in the villages that are within the radius of 10km is 58 years, while it is 59 years in villages that are beyond the 10km radius. In villages within the radius of 10km, 86.08 percent of respondents are unemployed, 5.70 percent are employed at the scheme and 8.23 percent are self-employed. In villages beyond 10km, 84.62 percent of respondents are unemployed, 5.13 percent are employed at the scheme and 10.26 percent are self-employed. In both villages, 71 percent of respondents reported that that they do not get feed supply from the scheme while 29 percent of respondents reported that they get feed supply from the scheme. From the overall distribution of villages within and beyond the radius of 10km, 80 percent of respondents reported that they do not get job opportunities from the QIS while 20 percent of respondents reported that they get job opportunities from the QIS. The study shows that 53 percent of respondents from villages within the radius of 10km are low-benefitting, 33 percent of respondents are intermediate-benefitting and 14 percent of respondents are high-benefitting from the scheme, while in villages beyond the radius of 10km, 97 percent of respondents are low-benefitting, 3 percent of respondents are intermediate-benefitting and none of the respondents is high-benefitting from the scheme. Furthermore, the study, through the multinomial logit regression model, shows that gender for intermediate benefitting has a negative coefficient (-1.70) and shows a significance of 10 percent On the other hand, gender for high benefitting has a positive coefficient (2.57) and shows a significance of 1 percent. Distance from the scheme has a negative coefficient (-3.08) and shows a significance of 1 percent. Access to farmland has a positive coefficient (2.33) and shows a significance of 10 percent. The study recommends that surrounding communities should own home gardens so that they can grow fresh produce and create job opportunities. The surrounding communities are also encouraged to own more farmlands or produce their own crops so they can also learn from the skills of the scheme.
- Full Text:
Species composition and seasonal population dynamics of free-living and engorged cattle ticks in three agro-ecological zones of the Eastern Cape Province, South Africa
- Authors: Yawa, Mandla
- Date: 2017
- Subjects: Ticks Boophilus microplus
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/9067 , vital:34224
- Description: This study was conducted to determine species composition and seasonal population dynamics of free-living and engorged cattle ticks in three agro-ecological zones (Bedford dry grassland, Kowie thicket and Bisho thornveld) of the Eastern Cape Province, South Africa. The occurrence of ticks on cattle was studied monthly from April 2016 and March 2017. Tick collections were performed on 360 selected cattle in each agro-ecological zones. During tick collections, 10 randomly selected cattle were sampled at each locality and more attention was given to African blue tick and the presence of Asian blue tick. A half body tick collection was performed from cattle and visible ticks were collected by means of fine-forceps after the animals had been restrained. Body regions that were examined includes the ear, head, neck, chest, abdomen, flank, front and hind leg and feet, tail, and peri-anal region. At each locality, six replicate drags of 100 m, approximately 50 m apart, were performed to collect ticks from vegetation. All ticks collected per cattle and from vegetation were stored in labelled sample tubes containing 70percent ethanol, subsequently counted and identified to genus and species level using a standard stereomicroscope. A total of 31425 ticks belonging to ten species were collected during the study. Rhipicephalus (Boophilus) decoloratus, was the most observed tick species (32.50percent), all other ticks like R. evertsi evertsi (18.84percent), R. appendiculatus (17.26percent), A. hebraeum (16.27percent), R. simus (7.69percent) were also commonly collected. Ixodes pilosus (3.84percent), H. rufipes (3.46percent), R. follis (0.08percent) together with Haemaphysalis silacea (0.02percent) were sporadic infestation. Haemaphysalis elliptica (0.04percent) was only found on the vegetation. No H. rufipes was collected in the thicket vegetation. Agro-ecological zones differ significantly (P<0.05) in tick species and distribution. Significantly more R. (B.) decoloratus were collected in thicket during summer compared to thornveld and dry grassland regions (P<0.05). Free-living ticks were widely distributed across different seasons but with of R. (B.) decoloratus larvae were significantly higher (P<0.05) in thornveld region during the spring season. In conclusion, agro-ecological differences and seasonal variations had an influence on ticks abundance and distribution. From this study, it is of interest to note the absence of R. (B.) microplus, whose trend to invade new areas previously colonised by R. (B.) decoloratus has been observed in Africa. In conclusion, agro-ecological differences and seasonal variations had an influence on ticks abundance and distribution. The aforementioned absence of R. (B.) microplus in this study highlights the importance of further research as this tick invades new areas previously colonised by R. (B.) decoloratus in the continent.
- Full Text:
- Authors: Yawa, Mandla
- Date: 2017
- Subjects: Ticks Boophilus microplus
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/9067 , vital:34224
- Description: This study was conducted to determine species composition and seasonal population dynamics of free-living and engorged cattle ticks in three agro-ecological zones (Bedford dry grassland, Kowie thicket and Bisho thornveld) of the Eastern Cape Province, South Africa. The occurrence of ticks on cattle was studied monthly from April 2016 and March 2017. Tick collections were performed on 360 selected cattle in each agro-ecological zones. During tick collections, 10 randomly selected cattle were sampled at each locality and more attention was given to African blue tick and the presence of Asian blue tick. A half body tick collection was performed from cattle and visible ticks were collected by means of fine-forceps after the animals had been restrained. Body regions that were examined includes the ear, head, neck, chest, abdomen, flank, front and hind leg and feet, tail, and peri-anal region. At each locality, six replicate drags of 100 m, approximately 50 m apart, were performed to collect ticks from vegetation. All ticks collected per cattle and from vegetation were stored in labelled sample tubes containing 70percent ethanol, subsequently counted and identified to genus and species level using a standard stereomicroscope. A total of 31425 ticks belonging to ten species were collected during the study. Rhipicephalus (Boophilus) decoloratus, was the most observed tick species (32.50percent), all other ticks like R. evertsi evertsi (18.84percent), R. appendiculatus (17.26percent), A. hebraeum (16.27percent), R. simus (7.69percent) were also commonly collected. Ixodes pilosus (3.84percent), H. rufipes (3.46percent), R. follis (0.08percent) together with Haemaphysalis silacea (0.02percent) were sporadic infestation. Haemaphysalis elliptica (0.04percent) was only found on the vegetation. No H. rufipes was collected in the thicket vegetation. Agro-ecological zones differ significantly (P<0.05) in tick species and distribution. Significantly more R. (B.) decoloratus were collected in thicket during summer compared to thornveld and dry grassland regions (P<0.05). Free-living ticks were widely distributed across different seasons but with of R. (B.) decoloratus larvae were significantly higher (P<0.05) in thornveld region during the spring season. In conclusion, agro-ecological differences and seasonal variations had an influence on ticks abundance and distribution. From this study, it is of interest to note the absence of R. (B.) microplus, whose trend to invade new areas previously colonised by R. (B.) decoloratus has been observed in Africa. In conclusion, agro-ecological differences and seasonal variations had an influence on ticks abundance and distribution. The aforementioned absence of R. (B.) microplus in this study highlights the importance of further research as this tick invades new areas previously colonised by R. (B.) decoloratus in the continent.
- Full Text:
Stem cutting propagation protocol for rose-scented geranium (Pelargonium graveolens)
- Authors: Matafeni, Ntombekhaya
- Date: 2017
- Subjects: Plant propagation Pelargoniums Plant cuttings
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/9832 , vital:35060
- Description: Rose-scented geranium (Pelargonium graveolens), is a high value essential oil plant that is used in the perfumery, cosmetic, aromatherapy and food flavouring industries. The increasing demand for this plant, due to its economic importance necessitates the development of an efficient propagation protocol for quality seedling and its maximum production. The present study therefore, sought to develop effective stem cutting propagation protocol which could facilitate multiplication of rose-scented geranium stem cuttings. Three separate experiments were undertaken to determine factors influencing effective propagation of rose-scented geranium. These factors were: rooting media, rooting hormone, cutting length and wound healing period on rooting and development of rose-scented geranium stem cuttings. The cuttings were assessed based on root number, length and fresh mass, plant height, leaf number, and stem circumference. In terms of root measurements, the growing media were washed out from the root system of plantlets, their roots were separated from stem before data was recorded which comprised of root number, length and root fresh mass. Root holding ability (RHA) on rooting medium was determined by visual observation and rated on a 1-5 scale where 1 = very loose, not acceptable; 2 = loose, not acceptable; 3 = medium, marginally acceptable; 4 = tight, acceptable; 5 = very tight, acceptable. The experiments were carried out at Essential Amatole Nursery, at the University of Fort Hare Research Farm, Alice Campus (located at 320 47ʹ3ʺS, 260 50ʹ43ʺ E, with an altitude of 519 m.a.s.l). All the experiments were carried out under mist conditions on bottom-heated beds in a greenhouse (with polycarbonate roofing of about 40percent shading effect) for the first three weeks after sticking the cuttings to the growing medium to facilitate root induction in relatively high temperature and relative humidity. Thereafter, the plantlets were grown in a shade house with 70percent light penetration until the termination of the experiment. To optimize the technology for the propagation of this plant the present experiment was designed with the objective to determine the efficient growing medium and proper rooting hormone for successful rooting and development of quality seedlings of rose-scented geranium. The experiment was set up in a complete randomized design (CRD) and was replicated three times with two factors 7×4, seven different growing media i.e. (1) Mixture growing medium which serves as control (pine bark 8 bags + sand 2 bags + lime 4kg + coconut 10 blocks + talborne 6.25 kg + bone meal 2 kg); (2) River sand only; (3) Pine bark; (4) Hygrotex (commercial rooting media); (5) Pine bark + river sand ( at1:1 ratio on volume basis); (6) Pine bark + hygrotex (at 1:1, ratio on volume basis), and; (7) Pine bark + river sand + hygrotex ( at 1:1:1 ratio on volume basis) and four different IBA hormone levels (auxins, types of IBA) applied as treatment were (1) Dynaroot (1 – 1g/kg), (2) Dynaroot (2- 3g/kg), (3) Dynaroot (3-8g/kg) and (4) Control (untreated with hormone). Hygrotex was identified as the best growth media for quicker regeneration giving the highest root number, length and fresh mass. While, hygrotex + pine bark (v/v 1:1) was efficient in producing more leaves, stem circumference and other aerial parameters. Dynaroot 3 was identified as the best rooting enhancer with maximum root number, length, fresh weight and plant height. Both Dynaroot 3 and Dynaroot 2 did not have major differences on giving highest leaf number. Control (untreated with hormone) was consistent in giving the greatest stem circumference than any other treatment. To maximize stem circumference, a combination of control (untreated with hormone) and hygrotex + pine bark (v/v 1:1) was identified as the best treatment. Based on the investigation for maximum production and quality seedlings of rose-scented geranium, hygrotex and Dynaroot 3 were identified as the best combination for successful rooting. The ideal cutting stem length, rooting hormone and growing medium for quality seedlings of rose-scented geranium were also investigated. The experimental lay out was in randomized complete design (RCD) with a 4 × 4 × 2 factorial treatment combination. Treatments used were, four different cutting lengths viz. 10, 12, 14 and 16 cm long; four different concentrations of IBA rooting hormone (Dynaroot 1, 2, 3 (powder form) and distilled water (control) and two types of growing medium (hygrotex and hygrotex + pine bark v/v 1:1) were used. Stem cuttings of 14 and 16 cm length gave the highest root number of 34, 38 and 35.13, and root length of 3.40 and 3.51cm respectively, with no significance. Cutting length of 10 cm favoured stem circumference (3.1 cm) as compared to other treatments. Whereas, cuttings treated with Dynaroot 3 showed a better root number (33.46 roots), root length (3.54 cm), root fresh mass (0.59 mg), leaf number of (11.08) as well as highest root holding ability (5). However, they showed no significance difference with Dynaroot 2 treated cuttings. In addition, cuttings treated with Control favoured shoot number (3.79) and stem circumference (3.05). Visually, hygrotex was observed to be better substrate though it was not significantly different from hygrotex + pine bark (1:1 v/v) on propagation of rose-scented geranium stem cuttings. Therefore, it is recommended that rose-scented geranium should be propagated through the combination of 14 cm cuttings length and treated with Dynaroot 2 IBA rooting hormone. Both hygrotex and hygrotex + pine bark (1:1 v/v) are the best growing media for root formation and growth of rose-scented geranium, though hygrotex alone is more economical. Wound healing period (WHP) of stem cutting was evaluated using 4 × 4 factorial, cuttings were separated into four groups during the healing duration (intervals of 24 hrs: days 0, 1, 2 and 3). These four groups were further subdivided into four subgroups of rooting hormone viz. Dynaroot 1, 2, 3 of indole-3-butyric acid and control (water). Experiment was laid out in a randomised complete block design (RCBD) with three replicates. The results obtained from the study revealed that rose-scented geranium rooted easily when planted on Day 2 of the wound healing period such that root holding ability was at its highest. While, Day 0 cuttings showed good response for stem circumference and shoot number. The study recommends that rose-scented geranium be propagated using cuttings that have enough time to heal the wound that is, Day 2 cuttings. Dynaroot 3 (IBA hormone concentration) showed good response to rooting and other arial parameters except for stem circumference which was favoured by application of control. Based on the results of the study, it can be concluded that propagation of rose-scented geranium requires a wound healing period of about three days in room temperature and application of IBA hormone before sucking cuttings in growing medium. Instead, of Dynaroot 3 or 2, Dynaroot 1 can also be used because, it is less economical and they all have a similar effect on cuttings that have been healing for three days.
- Full Text:
- Authors: Matafeni, Ntombekhaya
- Date: 2017
- Subjects: Plant propagation Pelargoniums Plant cuttings
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/9832 , vital:35060
- Description: Rose-scented geranium (Pelargonium graveolens), is a high value essential oil plant that is used in the perfumery, cosmetic, aromatherapy and food flavouring industries. The increasing demand for this plant, due to its economic importance necessitates the development of an efficient propagation protocol for quality seedling and its maximum production. The present study therefore, sought to develop effective stem cutting propagation protocol which could facilitate multiplication of rose-scented geranium stem cuttings. Three separate experiments were undertaken to determine factors influencing effective propagation of rose-scented geranium. These factors were: rooting media, rooting hormone, cutting length and wound healing period on rooting and development of rose-scented geranium stem cuttings. The cuttings were assessed based on root number, length and fresh mass, plant height, leaf number, and stem circumference. In terms of root measurements, the growing media were washed out from the root system of plantlets, their roots were separated from stem before data was recorded which comprised of root number, length and root fresh mass. Root holding ability (RHA) on rooting medium was determined by visual observation and rated on a 1-5 scale where 1 = very loose, not acceptable; 2 = loose, not acceptable; 3 = medium, marginally acceptable; 4 = tight, acceptable; 5 = very tight, acceptable. The experiments were carried out at Essential Amatole Nursery, at the University of Fort Hare Research Farm, Alice Campus (located at 320 47ʹ3ʺS, 260 50ʹ43ʺ E, with an altitude of 519 m.a.s.l). All the experiments were carried out under mist conditions on bottom-heated beds in a greenhouse (with polycarbonate roofing of about 40percent shading effect) for the first three weeks after sticking the cuttings to the growing medium to facilitate root induction in relatively high temperature and relative humidity. Thereafter, the plantlets were grown in a shade house with 70percent light penetration until the termination of the experiment. To optimize the technology for the propagation of this plant the present experiment was designed with the objective to determine the efficient growing medium and proper rooting hormone for successful rooting and development of quality seedlings of rose-scented geranium. The experiment was set up in a complete randomized design (CRD) and was replicated three times with two factors 7×4, seven different growing media i.e. (1) Mixture growing medium which serves as control (pine bark 8 bags + sand 2 bags + lime 4kg + coconut 10 blocks + talborne 6.25 kg + bone meal 2 kg); (2) River sand only; (3) Pine bark; (4) Hygrotex (commercial rooting media); (5) Pine bark + river sand ( at1:1 ratio on volume basis); (6) Pine bark + hygrotex (at 1:1, ratio on volume basis), and; (7) Pine bark + river sand + hygrotex ( at 1:1:1 ratio on volume basis) and four different IBA hormone levels (auxins, types of IBA) applied as treatment were (1) Dynaroot (1 – 1g/kg), (2) Dynaroot (2- 3g/kg), (3) Dynaroot (3-8g/kg) and (4) Control (untreated with hormone). Hygrotex was identified as the best growth media for quicker regeneration giving the highest root number, length and fresh mass. While, hygrotex + pine bark (v/v 1:1) was efficient in producing more leaves, stem circumference and other aerial parameters. Dynaroot 3 was identified as the best rooting enhancer with maximum root number, length, fresh weight and plant height. Both Dynaroot 3 and Dynaroot 2 did not have major differences on giving highest leaf number. Control (untreated with hormone) was consistent in giving the greatest stem circumference than any other treatment. To maximize stem circumference, a combination of control (untreated with hormone) and hygrotex + pine bark (v/v 1:1) was identified as the best treatment. Based on the investigation for maximum production and quality seedlings of rose-scented geranium, hygrotex and Dynaroot 3 were identified as the best combination for successful rooting. The ideal cutting stem length, rooting hormone and growing medium for quality seedlings of rose-scented geranium were also investigated. The experimental lay out was in randomized complete design (RCD) with a 4 × 4 × 2 factorial treatment combination. Treatments used were, four different cutting lengths viz. 10, 12, 14 and 16 cm long; four different concentrations of IBA rooting hormone (Dynaroot 1, 2, 3 (powder form) and distilled water (control) and two types of growing medium (hygrotex and hygrotex + pine bark v/v 1:1) were used. Stem cuttings of 14 and 16 cm length gave the highest root number of 34, 38 and 35.13, and root length of 3.40 and 3.51cm respectively, with no significance. Cutting length of 10 cm favoured stem circumference (3.1 cm) as compared to other treatments. Whereas, cuttings treated with Dynaroot 3 showed a better root number (33.46 roots), root length (3.54 cm), root fresh mass (0.59 mg), leaf number of (11.08) as well as highest root holding ability (5). However, they showed no significance difference with Dynaroot 2 treated cuttings. In addition, cuttings treated with Control favoured shoot number (3.79) and stem circumference (3.05). Visually, hygrotex was observed to be better substrate though it was not significantly different from hygrotex + pine bark (1:1 v/v) on propagation of rose-scented geranium stem cuttings. Therefore, it is recommended that rose-scented geranium should be propagated through the combination of 14 cm cuttings length and treated with Dynaroot 2 IBA rooting hormone. Both hygrotex and hygrotex + pine bark (1:1 v/v) are the best growing media for root formation and growth of rose-scented geranium, though hygrotex alone is more economical. Wound healing period (WHP) of stem cutting was evaluated using 4 × 4 factorial, cuttings were separated into four groups during the healing duration (intervals of 24 hrs: days 0, 1, 2 and 3). These four groups were further subdivided into four subgroups of rooting hormone viz. Dynaroot 1, 2, 3 of indole-3-butyric acid and control (water). Experiment was laid out in a randomised complete block design (RCBD) with three replicates. The results obtained from the study revealed that rose-scented geranium rooted easily when planted on Day 2 of the wound healing period such that root holding ability was at its highest. While, Day 0 cuttings showed good response for stem circumference and shoot number. The study recommends that rose-scented geranium be propagated using cuttings that have enough time to heal the wound that is, Day 2 cuttings. Dynaroot 3 (IBA hormone concentration) showed good response to rooting and other arial parameters except for stem circumference which was favoured by application of control. Based on the results of the study, it can be concluded that propagation of rose-scented geranium requires a wound healing period of about three days in room temperature and application of IBA hormone before sucking cuttings in growing medium. Instead, of Dynaroot 3 or 2, Dynaroot 1 can also be used because, it is less economical and they all have a similar effect on cuttings that have been healing for three days.
- Full Text:
Synthesis and characterization of CdSe quantum dots for solar cell application
- Authors: Makinana, Sinovuyo
- Date: 2017
- Subjects: Quantum dots Quantum dots -- Optical properties Renewable energy sources
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/5994 , vital:29466
- Description: This study shows a detailed report on the morphological, structural and optical properties of CdSe QDs synthesised by the hot injection method. Cadmium acetate dihydrate and Se powder were used as cadmium and selenide precursors, respectively. Various QD sizes were achieved by synthesizing in temperature range of 150ºC, 175ºC, 200ºC, 225ºC, 250ºC, 275ºC and 300ºC, respectively. The as synthesized QDs by the hot injection method were cross-examined for their morphological, structural and optical using HRTEM, FTIR, XRD, RS, and UV-Vis spectroscopy techniques respectively. FTIR analysis has revealed vibrations at 738, 738, 738, 738, 735, 735 and 733 cm-1 for the QDs synthesized at various temperatures of 150, 175, 200, 225, 250, 275, and 300℃, respectively. The presence of the above mentioned peaks confirms the presence of Cd-Se bond in our samples. XRD analysis of CdSe QDs revealed diffraction peaks at 2 angles of 16.66 , 25.20 , 34.77 , 40.9 , 45.39 and 49.1 for 150 17.4 , 25.22 , 34.85 , 41.7 , 44.45 and 47.5 for the QDs synthesized at various temperatures of 175 17.07 , 25.19 , 34.85 , 41.34 , 44.41 and 48.86 for 200 ; 16.34 , 25.20 , 34.76 , 40.6 , 44.74 and 49.48 for 225 ; 17.44 , 25.17 , 34.19 , 41.7 , 44.45 , 49.24 for 250 ; 16.70 , 25.16 , 34.85 , 40.32 , 45.1 and 49.1 7 for 275 ;and 17.35 , 25.18 , 35.13 , 41.63 , 45.7 , 49.48 for 300 . These XRD peaks relate to crystal planes of (100), (002), (102), (220), (103) and (112) which belong to hexagonal Wurtzite CdSe crystal structure. Additionally XRD analysis has revealed a general peak shift to higher 2 values was observed for CdSe QDs. HRTEM analysis showed that the synthesised CdSe QDs have a spherical shape and are monodispersed. Moreover, HRTEM analysis has revealed CdSe QDs modal crystallite size of 1.79 nm, 1.81 nm, 2.06 nm, 2.08 nm, 2.11 nm, 3.10 nm and 3.12 nm for the QDs synthesized at various temperatures of 150ºC, 175ºC, 200ºC, 225ºC, 250ºC, 275ºC and 300ºC, respectively. HRTEM results were in mutual agreement with XRD results. Additionally, the SAED images showed intense electron diffraction rings, which confirmed that the as-synthesised CdSe QDs have a Wurtzite crystal structure. RS analysis showed that CdSe QDs have LO and 2LO vibrational modes which are characteristic peaks for CdSe. The presence of these peaks in Raman spectra further supports our previous observation from XRD analysis and HRTEM analysis that the synthesized CdSe QDs have a Wurtzite crystal structure. The effect of synthesis temperature Raman peak shift, FHWH and peak intensity has been cross examined in this work, Moreover, the effect of increasing temperature on the peak shift, FWHM and peak intensity is discussed in detail below. UV-Vis analysis revealed an absorbance of CdSe QDs in higher wavelengths as temperature was increased. Furthermore, the Yu et al 2003 relation was used to calculate QD size and band gap energy of CdSe QDs. The results showed that QD size increases with increasing synthesis temperature, which is in agreement with HRTEM and XRD results.
- Full Text:
- Authors: Makinana, Sinovuyo
- Date: 2017
- Subjects: Quantum dots Quantum dots -- Optical properties Renewable energy sources
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/5994 , vital:29466
- Description: This study shows a detailed report on the morphological, structural and optical properties of CdSe QDs synthesised by the hot injection method. Cadmium acetate dihydrate and Se powder were used as cadmium and selenide precursors, respectively. Various QD sizes were achieved by synthesizing in temperature range of 150ºC, 175ºC, 200ºC, 225ºC, 250ºC, 275ºC and 300ºC, respectively. The as synthesized QDs by the hot injection method were cross-examined for their morphological, structural and optical using HRTEM, FTIR, XRD, RS, and UV-Vis spectroscopy techniques respectively. FTIR analysis has revealed vibrations at 738, 738, 738, 738, 735, 735 and 733 cm-1 for the QDs synthesized at various temperatures of 150, 175, 200, 225, 250, 275, and 300℃, respectively. The presence of the above mentioned peaks confirms the presence of Cd-Se bond in our samples. XRD analysis of CdSe QDs revealed diffraction peaks at 2 angles of 16.66 , 25.20 , 34.77 , 40.9 , 45.39 and 49.1 for 150 17.4 , 25.22 , 34.85 , 41.7 , 44.45 and 47.5 for the QDs synthesized at various temperatures of 175 17.07 , 25.19 , 34.85 , 41.34 , 44.41 and 48.86 for 200 ; 16.34 , 25.20 , 34.76 , 40.6 , 44.74 and 49.48 for 225 ; 17.44 , 25.17 , 34.19 , 41.7 , 44.45 , 49.24 for 250 ; 16.70 , 25.16 , 34.85 , 40.32 , 45.1 and 49.1 7 for 275 ;and 17.35 , 25.18 , 35.13 , 41.63 , 45.7 , 49.48 for 300 . These XRD peaks relate to crystal planes of (100), (002), (102), (220), (103) and (112) which belong to hexagonal Wurtzite CdSe crystal structure. Additionally XRD analysis has revealed a general peak shift to higher 2 values was observed for CdSe QDs. HRTEM analysis showed that the synthesised CdSe QDs have a spherical shape and are monodispersed. Moreover, HRTEM analysis has revealed CdSe QDs modal crystallite size of 1.79 nm, 1.81 nm, 2.06 nm, 2.08 nm, 2.11 nm, 3.10 nm and 3.12 nm for the QDs synthesized at various temperatures of 150ºC, 175ºC, 200ºC, 225ºC, 250ºC, 275ºC and 300ºC, respectively. HRTEM results were in mutual agreement with XRD results. Additionally, the SAED images showed intense electron diffraction rings, which confirmed that the as-synthesised CdSe QDs have a Wurtzite crystal structure. RS analysis showed that CdSe QDs have LO and 2LO vibrational modes which are characteristic peaks for CdSe. The presence of these peaks in Raman spectra further supports our previous observation from XRD analysis and HRTEM analysis that the synthesized CdSe QDs have a Wurtzite crystal structure. The effect of synthesis temperature Raman peak shift, FHWH and peak intensity has been cross examined in this work, Moreover, the effect of increasing temperature on the peak shift, FWHM and peak intensity is discussed in detail below. UV-Vis analysis revealed an absorbance of CdSe QDs in higher wavelengths as temperature was increased. Furthermore, the Yu et al 2003 relation was used to calculate QD size and band gap energy of CdSe QDs. The results showed that QD size increases with increasing synthesis temperature, which is in agreement with HRTEM and XRD results.
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Synthesis of modified zinc oxide nanoparticles using pneumatic spray pyrolysis for solar cell application
- Authors: Ntozakhe, Luyolo
- Date: 2017
- Subjects: Zinc oxide -- Synthesis Nanoparticles
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/5862 , vital:29408
- Description: In this work, the pneumatic spray pyrolysis was used to synthesize un-doped and carbon doped zinc oxide (ZnO) nanoparticles. The zinc acetate, tetrabutylammonium bromide and ethanol were used as starting materials for the desired ZnO nanoparticles and the prepared samples were annealed at 400 oC in the furnace. The as synthesized un-doped and carbon doped ZnO NPs were evaluated using X-ray diffraction (XRD), Scanning electron microscope (SEM), Energy dispersive x-ray spectroscopy (EDX), High-resolution transmission electron microscopy (HRTEM), Raman spectroscopy (RS) and Ultraviolet-visible spectroscopy (UV-Vis). XRD analysis of the synthesized NPs revealed peaks at 31.90°, 34.50°, 36.34°, 47.73°, 56.88°, 63.04°, 68.20°, and 77.33° belonging to the hexagonal Wurtzite ZnO crystal structure. The incorporation of C species into ZnO lattice was cross examined by monitoring the peak positions of the (100), (002) and (001) planes. These three main peaks of C-ZnO NPs show a peak shift to higher 2θ values which indicates substitutional carbon doping in ZnO NPs. SEM analysis has revealed that the as synthesized NPs have spherical shape and the morphology of the NPs change as the concentration of carbon increases. The EDX spectra of both un-doped and doped ZnO nanoparticles have revealed prominent peaks at 0.51 keV, 1.01 keV, 1.49 keV, 8.87 keV and 9.86 keV. Peaks at, X-ray energies of 0.51 keV and 1.01 keV respectively represent the emissions from the K-shell of oxygen and L-shell of zinc. The L-shell emission at 1.01 keV is considered as convolution of Zn 2p3/2 and Zn 2p1/2 photoelectron energies. The occurrence of these peaks in the EDX endorses the existence of Zn and O atoms in the PSP prepared samples. HRTEM analysis has revealed NPs size modal range from 6.65-14.21 nm for the PSP synthesized samples which is in mutual agreement with the XRD data calculated values. More over the selected area diffraction images displaying the fact that only the diffraction planes of (101), (002) and (100) are responsible for the diffraction pattern belonging to Wurtzite ZnO. RS analysis has revealed that the un-doped ZnO and doped ZnO samples have characteristic Raman vibration modes at 325 cm-1, and 434 cm-1 belonging to Wurtzite ZnO structure. Moreover, the prominent peak at 434 cm-1 which is the characteristic peak of E2(2) (high) mode of the Wurtzite ZnO and the E2(2) (high) has been red shifted by 4 cm-1, as compared to that found in the bulk ZnO. Additionally, the effect of carbon doping through Raman spectroscopy peak shifts of the E2(2) (high) mode, A1(LO) mode and multi-phonon has also been considered and discussed in detail. UV-Vis diffuse reflectance spectroscopy has revealed a red shift of the absorption edge with increase in C doping. Finally, the effect of nano-crystallite size and gradual prominence of C into ZnO lattice due to increase in C doping concentration in the PSP prepared nanoparticles was meticulously elaborated through Raman Spectroscopy analysis.
- Full Text:
- Authors: Ntozakhe, Luyolo
- Date: 2017
- Subjects: Zinc oxide -- Synthesis Nanoparticles
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/5862 , vital:29408
- Description: In this work, the pneumatic spray pyrolysis was used to synthesize un-doped and carbon doped zinc oxide (ZnO) nanoparticles. The zinc acetate, tetrabutylammonium bromide and ethanol were used as starting materials for the desired ZnO nanoparticles and the prepared samples were annealed at 400 oC in the furnace. The as synthesized un-doped and carbon doped ZnO NPs were evaluated using X-ray diffraction (XRD), Scanning electron microscope (SEM), Energy dispersive x-ray spectroscopy (EDX), High-resolution transmission electron microscopy (HRTEM), Raman spectroscopy (RS) and Ultraviolet-visible spectroscopy (UV-Vis). XRD analysis of the synthesized NPs revealed peaks at 31.90°, 34.50°, 36.34°, 47.73°, 56.88°, 63.04°, 68.20°, and 77.33° belonging to the hexagonal Wurtzite ZnO crystal structure. The incorporation of C species into ZnO lattice was cross examined by monitoring the peak positions of the (100), (002) and (001) planes. These three main peaks of C-ZnO NPs show a peak shift to higher 2θ values which indicates substitutional carbon doping in ZnO NPs. SEM analysis has revealed that the as synthesized NPs have spherical shape and the morphology of the NPs change as the concentration of carbon increases. The EDX spectra of both un-doped and doped ZnO nanoparticles have revealed prominent peaks at 0.51 keV, 1.01 keV, 1.49 keV, 8.87 keV and 9.86 keV. Peaks at, X-ray energies of 0.51 keV and 1.01 keV respectively represent the emissions from the K-shell of oxygen and L-shell of zinc. The L-shell emission at 1.01 keV is considered as convolution of Zn 2p3/2 and Zn 2p1/2 photoelectron energies. The occurrence of these peaks in the EDX endorses the existence of Zn and O atoms in the PSP prepared samples. HRTEM analysis has revealed NPs size modal range from 6.65-14.21 nm for the PSP synthesized samples which is in mutual agreement with the XRD data calculated values. More over the selected area diffraction images displaying the fact that only the diffraction planes of (101), (002) and (100) are responsible for the diffraction pattern belonging to Wurtzite ZnO. RS analysis has revealed that the un-doped ZnO and doped ZnO samples have characteristic Raman vibration modes at 325 cm-1, and 434 cm-1 belonging to Wurtzite ZnO structure. Moreover, the prominent peak at 434 cm-1 which is the characteristic peak of E2(2) (high) mode of the Wurtzite ZnO and the E2(2) (high) has been red shifted by 4 cm-1, as compared to that found in the bulk ZnO. Additionally, the effect of carbon doping through Raman spectroscopy peak shifts of the E2(2) (high) mode, A1(LO) mode and multi-phonon has also been considered and discussed in detail. UV-Vis diffuse reflectance spectroscopy has revealed a red shift of the absorption edge with increase in C doping. Finally, the effect of nano-crystallite size and gradual prominence of C into ZnO lattice due to increase in C doping concentration in the PSP prepared nanoparticles was meticulously elaborated through Raman Spectroscopy analysis.
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Template-assisted sol-gel synthesis of carbon doped titanium dioxide nanotubes and their characterization
- Authors: Takata, Nwabisa
- Date: 2017
- Subjects: Nanotechnology Titanium dioxide Nanostructures
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/13194 , vital:39473
- Description: This study reveals the effects of doping on the morphological, structural and optical properties of titanium dioxide (TiO2) nanotubes (TNTs), synthesized by sol-gel template-assisted sol-gel technique. The nanotubes (TNTs) were prepared in anodic alumina membranes (AAM) with a pore diameter range of 110-210 nm by using titanium tetra butoxide as a sol-gel precursor and oxalic acid dihydrate as a dopant source. The synthesized nanotubes were evaluated using scanning electron microscope (SEM), energy dispersive spectroscopy (EDX), fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), confocal Raman spectroscopy (CRS) and photoluminescence spectroscopy (PL). SEM analysis has revealed the presence of closely-packed TNTs, with a modal external tube diameters of 150, 170, 210,190 and 210 nm for the un-doped TNTs, 9 mM carbon doped-TNTs (C-TNTs), 27 mM C-TNTs, 45 mM C-TNTs and 75 mM C-TNTs respectively. The diameters are consistent with the AAM diameter range. EDX spectra revealed the presence of Ti peaks at 0.45 and 4.9 keV corresponding to Kα1 and Kβ1 emission lines respectively. Oxygen exhibits a signal at 0.5 keV corresponding to Kα1 emission line. The occurrence of these peaks in the EDX spectra endorses the existence of Ti and O atoms in the prepared titanium dioxide nanotubes. FTIR spectroscopy has revealed the presence of vibration modes at 580-660 cm-1 indicating the presence of Ti-O bonds and additional vibration modes at 2324 cm-1 resulting from C-O stretching in the C-TNTs. The XRD analysis has revealed the presence of a mixed anatase-brookite phase with diffraction peaks at 2θ angles of 25.49⁰, 38.11⁰, 40.60º, 48.14⁰, 54.58⁰, 63.00⁰, 70.11⁰ and 75.66⁰. Additionally, XRD analysis has revealed elongation of lattice parameter “c” from 9.143 to 9.830 Å with carbon concentration increase. Lattice expansion indicates the possibility of carbon substituting oxygen sites. Raman large area scan has revealed the presence of rutile, brookite and anatase for the undoped samples. On doping the rutile phase of TiO2 has shown to be suppressed by the presence of carbon atoms such that the doped samples consist of brookite and anatase phases only. The Eg1 mode of anatase of the undoped TNTs at 153. 78 cm-1 was red shifted by Δ9.78 cm-1 relative to the bulk anatase TiO2. This was attributed to decrease in particle size, presence of brookite and phonon confinement. Upon doping, the 9 mM C-TNTs, 27 mM TNTs and 75 mM TNTs have shown a red shift of Δ0. 09 cm-1, Δ1. 39 cm-1 and 1.81 cm-1 respectively, suggesting the incorporation of carbon in the TiO2 matrix. CRS depth profiling in the XZ direction has also validated the presence of a mixed anatase-brookite phase at Raman active modes 153.19 cm-1, 208.87 cm-1, 404.55 cm-1, 523.26 cm-1 and 648.55 cm-1. Photoluminescence spectra of carbon doped TiO2 showed two emission peaks at 398 nm attributed to annihilation of excitons while the broad peak at 400-460 nm was attributed to the presence of oxygen vacancies. The peak intensity of the 45 mM C-TNTs has shown a lower PL intensity suggesting that efficiency of charge separation was higher and recombination rate was lower than other carbon containing samples.
- Full Text:
- Authors: Takata, Nwabisa
- Date: 2017
- Subjects: Nanotechnology Titanium dioxide Nanostructures
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/13194 , vital:39473
- Description: This study reveals the effects of doping on the morphological, structural and optical properties of titanium dioxide (TiO2) nanotubes (TNTs), synthesized by sol-gel template-assisted sol-gel technique. The nanotubes (TNTs) were prepared in anodic alumina membranes (AAM) with a pore diameter range of 110-210 nm by using titanium tetra butoxide as a sol-gel precursor and oxalic acid dihydrate as a dopant source. The synthesized nanotubes were evaluated using scanning electron microscope (SEM), energy dispersive spectroscopy (EDX), fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), confocal Raman spectroscopy (CRS) and photoluminescence spectroscopy (PL). SEM analysis has revealed the presence of closely-packed TNTs, with a modal external tube diameters of 150, 170, 210,190 and 210 nm for the un-doped TNTs, 9 mM carbon doped-TNTs (C-TNTs), 27 mM C-TNTs, 45 mM C-TNTs and 75 mM C-TNTs respectively. The diameters are consistent with the AAM diameter range. EDX spectra revealed the presence of Ti peaks at 0.45 and 4.9 keV corresponding to Kα1 and Kβ1 emission lines respectively. Oxygen exhibits a signal at 0.5 keV corresponding to Kα1 emission line. The occurrence of these peaks in the EDX spectra endorses the existence of Ti and O atoms in the prepared titanium dioxide nanotubes. FTIR spectroscopy has revealed the presence of vibration modes at 580-660 cm-1 indicating the presence of Ti-O bonds and additional vibration modes at 2324 cm-1 resulting from C-O stretching in the C-TNTs. The XRD analysis has revealed the presence of a mixed anatase-brookite phase with diffraction peaks at 2θ angles of 25.49⁰, 38.11⁰, 40.60º, 48.14⁰, 54.58⁰, 63.00⁰, 70.11⁰ and 75.66⁰. Additionally, XRD analysis has revealed elongation of lattice parameter “c” from 9.143 to 9.830 Å with carbon concentration increase. Lattice expansion indicates the possibility of carbon substituting oxygen sites. Raman large area scan has revealed the presence of rutile, brookite and anatase for the undoped samples. On doping the rutile phase of TiO2 has shown to be suppressed by the presence of carbon atoms such that the doped samples consist of brookite and anatase phases only. The Eg1 mode of anatase of the undoped TNTs at 153. 78 cm-1 was red shifted by Δ9.78 cm-1 relative to the bulk anatase TiO2. This was attributed to decrease in particle size, presence of brookite and phonon confinement. Upon doping, the 9 mM C-TNTs, 27 mM TNTs and 75 mM TNTs have shown a red shift of Δ0. 09 cm-1, Δ1. 39 cm-1 and 1.81 cm-1 respectively, suggesting the incorporation of carbon in the TiO2 matrix. CRS depth profiling in the XZ direction has also validated the presence of a mixed anatase-brookite phase at Raman active modes 153.19 cm-1, 208.87 cm-1, 404.55 cm-1, 523.26 cm-1 and 648.55 cm-1. Photoluminescence spectra of carbon doped TiO2 showed two emission peaks at 398 nm attributed to annihilation of excitons while the broad peak at 400-460 nm was attributed to the presence of oxygen vacancies. The peak intensity of the 45 mM C-TNTs has shown a lower PL intensity suggesting that efficiency of charge separation was higher and recombination rate was lower than other carbon containing samples.
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The effect of fire management on chemical and physical properties of rangelands
- Authors: Magomani, Matimba Israel
- Date: 2017
- Subjects: Rangelands -- Fire management Soil ecology
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/6191 , vital:29506
- Description: The use of fire in rangeland has become standard practice for decades; however, there is no shared understanding of how fire affects soil properties. The exact relationship between fire and soil properties is still yet to be understood. The differences in fire frequency, fire intensity, fire severity, the period between fire and sampling resulted in contradictory results amongst studies. A long-term trial at the University of Fort Hare provides a valuable experimental layout for fire research. The trial was established in 1980 with the aim of investigating the effect of burning frequency on vegetation species composition and biomass production. The objective of this study was however to determine the effect of fire management on soil chemical and physical properties under the long-term burning experiment. The treatments of the study site were: no burn (K), annual (B1), biennial (B2), triennial (B3), quadrennial (B4) and sexennial (B6) burns. A line intercept sampling technique was used to collect the soil samples from the surface (0 to 75 mm) for both chemical and physical analysis. All Burning treatments significantly (p < 0.05) increased the bulk density (Db), soil pH, total cations, Magnesium (Mg) and Calcium (Ca), but reduced the soil porosity and exchangeable acidity when compared to the control. Hydraulic conductivity (Ks), water conducting macroporosity (WCM) and total nitrogen were reduced under more frequent burning treatments (B1, B2), as compared to less frequent burning treatments (B3, B4, B6). The aggregate stability showed an opposite trend. Total organic carbon (TOC) was reduced only under annual burning treatment. Burning frequencies did not impact the extractable Phosphorus (p), Potassium (K), Sodium, soil water repellency and water content significantly (P > 0.05). The risk associated with annual burning showed in this study might facilitate processes such as overflow that lead to soil degradation. Triennial burning in rangeland ecosystem supports soil conservation practices. Further studies that measure the quality of rangeland in triennial burning is necessary.
- Full Text:
- Authors: Magomani, Matimba Israel
- Date: 2017
- Subjects: Rangelands -- Fire management Soil ecology
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/6191 , vital:29506
- Description: The use of fire in rangeland has become standard practice for decades; however, there is no shared understanding of how fire affects soil properties. The exact relationship between fire and soil properties is still yet to be understood. The differences in fire frequency, fire intensity, fire severity, the period between fire and sampling resulted in contradictory results amongst studies. A long-term trial at the University of Fort Hare provides a valuable experimental layout for fire research. The trial was established in 1980 with the aim of investigating the effect of burning frequency on vegetation species composition and biomass production. The objective of this study was however to determine the effect of fire management on soil chemical and physical properties under the long-term burning experiment. The treatments of the study site were: no burn (K), annual (B1), biennial (B2), triennial (B3), quadrennial (B4) and sexennial (B6) burns. A line intercept sampling technique was used to collect the soil samples from the surface (0 to 75 mm) for both chemical and physical analysis. All Burning treatments significantly (p < 0.05) increased the bulk density (Db), soil pH, total cations, Magnesium (Mg) and Calcium (Ca), but reduced the soil porosity and exchangeable acidity when compared to the control. Hydraulic conductivity (Ks), water conducting macroporosity (WCM) and total nitrogen were reduced under more frequent burning treatments (B1, B2), as compared to less frequent burning treatments (B3, B4, B6). The aggregate stability showed an opposite trend. Total organic carbon (TOC) was reduced only under annual burning treatment. Burning frequencies did not impact the extractable Phosphorus (p), Potassium (K), Sodium, soil water repellency and water content significantly (P > 0.05). The risk associated with annual burning showed in this study might facilitate processes such as overflow that lead to soil degradation. Triennial burning in rangeland ecosystem supports soil conservation practices. Further studies that measure the quality of rangeland in triennial burning is necessary.
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The effect of phosphorus fertilizer application on growth, biomass and nutritive value of Pigeon pea (Cajanus cajan (L.) Millsp.) varieties in Alice, South Africa
- Authors: Mpabanga, Nkosi
- Date: 2017
- Subjects: Phosphatic fertilizers Soil fertility Plants -- Effect of phosphorus on
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/6180 , vital:29501
- Description: Low soil fertility has been identified to be among the major causes of low pasture quantity and quality. Scarcity of high quality forage mainly during the dry season threatens animal productivity in some areas of South Africa, thus the need for identifying livestock feed alternatives. Pigeon pea is a woody legume that has continually been an important shrub in agroforestry and can provide protein-rich livestock feed during times of shortage. Soil fertility to support the production of pigeon pea can be enhanced by application of fertilisers. Therefore, this study was carried out firstly, to determine the effect of phosphorus fertiliser on the growth performance and biomass production of three pigeon pea varieties, and secondly, to investigate the influence of phosphorus application on the nutrient and anti-nutrient content of three pigeon pea varieties. The field experiment was done at the University of Fort Hare research farm in Alice, South Africa. The factors investigated were three pigeon pea varieties namely Chitedze 1, Chitedze 2 and CIMMYT 1 and three phosphorus fertiliser application rates of 0, 40 and 60 kg P/ha. The study was a 3× 3 factorial experiment set up in a randomised complete block design and was replicated three times. The growth performance attributes evaluated in this study include the number of branches, plant height and root collar diameter. Root collar diameter was not significantly (p>0.05) different among the pigeon pea varieties and phosphorus levels. Likewise, the application of phosphorus was not significant (p>0.05) on plant height and the number of branches of all the varieties. However, when P level was varied, there were significant differences among the varieties, and so were the interaction (p<0.05) between variety and phosphorus on plant height and the number of branches. There was no significant (p>0.05) difference among the phosphorus levels on the fresh leaf yield of Chitedze 1 and Chitedze 2, but phosphorus application at 40 kg P/ha increased the fresh leaf yield of CIMMYT 1. Phosphorus application at 60 kg P/ha increased (p<0.05) the dry matter content of Chitedze 1 and Chitedze 2. Furthermore, the results of the trial show that the application of 40 kg P/ha and 60 kg P/ha increased (p<0.05) the dry matter yield of the varieties CIMMYT 1 and Chitedze 2, respectively. The effects of phosphorus application on the nutrient composition of the three pigeon pea varieties varied. The composition of hemicellulose of all the varieties was significantly (p<0.05) increased by the application of 40 kg P/ha. Conversely, the concentration of crude protein, phosphorus, copper and iron, and the calcium to phosphorus ratio of all the pigeon pea varieties was not significantly (p>0.05) affected by phosphorus application and there was no interactive effect of variety and phosphorus. The proximate compositions of fat, total non-structural carbohydrates, ash, neutral detergent fibre, acid detergent fibre, cellulose, neutral detergent insoluble nitrogen, acid detergent insoluble nitrogen of the pigeon pea varieties varied significantly (p<0.05) at different phosphorus application levels. There was an interactive (p<0.05) effect of variety and the level of phosphorus on the concentration of calcium (Ca), potassium (K), magnesium (Mg), sodium, zinc, manganese, and the K/(Ca+Mg) ratio. The digestible dry matter, dry matter intake and the relative feed value were significantly (p<0.05) different among the pigeon pea varieties at varying phosphorus levels. The anti-nutrient composition of Chitedze 2 was not significantly (p>0.05) affected by phosphorus application but the concentration of phenols and saponins of Chitedze 1 was significantly (p<0.05) increased when 60 kg P/ha was applied, but these anti-nutrients were reduced (p<0.05) for CIMMYT 1 at 60 kg P/ha. It can be concluded that phosphorus application influences the biomass production and chemical composition while the optimum phosphorus level vary among the pigeon pea varieties.
- Full Text:
- Authors: Mpabanga, Nkosi
- Date: 2017
- Subjects: Phosphatic fertilizers Soil fertility Plants -- Effect of phosphorus on
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/6180 , vital:29501
- Description: Low soil fertility has been identified to be among the major causes of low pasture quantity and quality. Scarcity of high quality forage mainly during the dry season threatens animal productivity in some areas of South Africa, thus the need for identifying livestock feed alternatives. Pigeon pea is a woody legume that has continually been an important shrub in agroforestry and can provide protein-rich livestock feed during times of shortage. Soil fertility to support the production of pigeon pea can be enhanced by application of fertilisers. Therefore, this study was carried out firstly, to determine the effect of phosphorus fertiliser on the growth performance and biomass production of three pigeon pea varieties, and secondly, to investigate the influence of phosphorus application on the nutrient and anti-nutrient content of three pigeon pea varieties. The field experiment was done at the University of Fort Hare research farm in Alice, South Africa. The factors investigated were three pigeon pea varieties namely Chitedze 1, Chitedze 2 and CIMMYT 1 and three phosphorus fertiliser application rates of 0, 40 and 60 kg P/ha. The study was a 3× 3 factorial experiment set up in a randomised complete block design and was replicated three times. The growth performance attributes evaluated in this study include the number of branches, plant height and root collar diameter. Root collar diameter was not significantly (p>0.05) different among the pigeon pea varieties and phosphorus levels. Likewise, the application of phosphorus was not significant (p>0.05) on plant height and the number of branches of all the varieties. However, when P level was varied, there were significant differences among the varieties, and so were the interaction (p<0.05) between variety and phosphorus on plant height and the number of branches. There was no significant (p>0.05) difference among the phosphorus levels on the fresh leaf yield of Chitedze 1 and Chitedze 2, but phosphorus application at 40 kg P/ha increased the fresh leaf yield of CIMMYT 1. Phosphorus application at 60 kg P/ha increased (p<0.05) the dry matter content of Chitedze 1 and Chitedze 2. Furthermore, the results of the trial show that the application of 40 kg P/ha and 60 kg P/ha increased (p<0.05) the dry matter yield of the varieties CIMMYT 1 and Chitedze 2, respectively. The effects of phosphorus application on the nutrient composition of the three pigeon pea varieties varied. The composition of hemicellulose of all the varieties was significantly (p<0.05) increased by the application of 40 kg P/ha. Conversely, the concentration of crude protein, phosphorus, copper and iron, and the calcium to phosphorus ratio of all the pigeon pea varieties was not significantly (p>0.05) affected by phosphorus application and there was no interactive effect of variety and phosphorus. The proximate compositions of fat, total non-structural carbohydrates, ash, neutral detergent fibre, acid detergent fibre, cellulose, neutral detergent insoluble nitrogen, acid detergent insoluble nitrogen of the pigeon pea varieties varied significantly (p<0.05) at different phosphorus application levels. There was an interactive (p<0.05) effect of variety and the level of phosphorus on the concentration of calcium (Ca), potassium (K), magnesium (Mg), sodium, zinc, manganese, and the K/(Ca+Mg) ratio. The digestible dry matter, dry matter intake and the relative feed value were significantly (p<0.05) different among the pigeon pea varieties at varying phosphorus levels. The anti-nutrient composition of Chitedze 2 was not significantly (p>0.05) affected by phosphorus application but the concentration of phenols and saponins of Chitedze 1 was significantly (p<0.05) increased when 60 kg P/ha was applied, but these anti-nutrients were reduced (p<0.05) for CIMMYT 1 at 60 kg P/ha. It can be concluded that phosphorus application influences the biomass production and chemical composition while the optimum phosphorus level vary among the pigeon pea varieties.
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The effect of quantitative feed restriction on growth performance, carcass characteristics and selected meat quality parameters in broiler chickens
- Authors: Velele, Siphelo
- Date: 2017
- Subjects: Broilers (Chickens) Poultry -- Breeding Meat -- Quality
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/8888 , vital:33907
- Description: The study investigated the effects of quantitative feed restriction on growth performance, carcass characteristics, internal organs, breast muscle pH and meat colour in broiler chickens. A total of 90, un-sexed, day-old broiler chicks were procured from an accredited supplier and were used for the current trial. For the first 14 days, all birds were brooded in one house and subjected to a uniform day-to-day management. Water was supplied throughout the trial, whereas, feed was only supplied ad libitum between days 1-14 and 29-35 days in feed restricted birds. Feed phases included starter (1-14 days), grower (15-28) and finisher (29-35), respectively. On day 15, birds were randomly allocated to three treatments; each treatment was replicated three times with 10 birds per replicate. The first treatment (T1) group, which acted as control group, was ad libitum feeding for the whole trial duration (1-35 days). For treatments 2 (T2) and 3(T3), 85 percent and 70 percent of the Cobb 500 broiler feed intake standards were applied for a period of 14 days (days 15-28), respectively. Average daily feed intake (ADFI) was determined daily and then average body weight (ABW) was determined weekly. Feed conversion ratio and average daily weight gain was thus computed for each experimental unit (replicate). On day 35, broiler birds were electrically stunned and slaughtered by a sharp knife and then hung for complete bleeding. Data collected included slaughter, carcass, breast, thigh, wing, drumstick, feet, head, spleen, heart, gizzard, intestines (small & large) and liver weights. Breast muscle was further used for the determination of colour (L*, b* and a*), initial (pHi) and ultimate breast pH (pHu) measurements. Although, quantitative feed restriction (T2 & T3) significantly affected growth performance in weeks 3 and 4, birds submitted to T2 performed similar (P>0.05) to control in the final stage, whilst birds in T3 was the poorest (P<0.05). Furthermore, birds subjected to T2 showed no differences of edible carcass portions to the control, however, T3 group showed lower (P<0.05) slaughter and wing weights. Breast ultimate pH values of birds under T2 were similar to control, but birds in T3 had significantly higher (P<0.05) values. Birds under T2 had less (P<0.05) reddish breast meat colour than control at 45 minutes. At 24 hours post-mortem, birds in T3 had lower (P<0.05) lightness values and birds submitted to T2 had higher (P<0.05) yellowness values. Liver weight was significantly lower in birds under T3 and higher (P<0.05) gizzard weights were found in birds subjected to T2. Birds under T2 performed similar to control and were able to compensate for the weight loss when high plane of feed was re-introduced. It can be concluded from the results that restricting 15 percent of feed from Cobb 500 standard feed intake had moderately affected broiler performance. Moreover, restricting 15percent of feed significantly reduced feed intake in broiler chicken.
- Full Text:
- Authors: Velele, Siphelo
- Date: 2017
- Subjects: Broilers (Chickens) Poultry -- Breeding Meat -- Quality
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/8888 , vital:33907
- Description: The study investigated the effects of quantitative feed restriction on growth performance, carcass characteristics, internal organs, breast muscle pH and meat colour in broiler chickens. A total of 90, un-sexed, day-old broiler chicks were procured from an accredited supplier and were used for the current trial. For the first 14 days, all birds were brooded in one house and subjected to a uniform day-to-day management. Water was supplied throughout the trial, whereas, feed was only supplied ad libitum between days 1-14 and 29-35 days in feed restricted birds. Feed phases included starter (1-14 days), grower (15-28) and finisher (29-35), respectively. On day 15, birds were randomly allocated to three treatments; each treatment was replicated three times with 10 birds per replicate. The first treatment (T1) group, which acted as control group, was ad libitum feeding for the whole trial duration (1-35 days). For treatments 2 (T2) and 3(T3), 85 percent and 70 percent of the Cobb 500 broiler feed intake standards were applied for a period of 14 days (days 15-28), respectively. Average daily feed intake (ADFI) was determined daily and then average body weight (ABW) was determined weekly. Feed conversion ratio and average daily weight gain was thus computed for each experimental unit (replicate). On day 35, broiler birds were electrically stunned and slaughtered by a sharp knife and then hung for complete bleeding. Data collected included slaughter, carcass, breast, thigh, wing, drumstick, feet, head, spleen, heart, gizzard, intestines (small & large) and liver weights. Breast muscle was further used for the determination of colour (L*, b* and a*), initial (pHi) and ultimate breast pH (pHu) measurements. Although, quantitative feed restriction (T2 & T3) significantly affected growth performance in weeks 3 and 4, birds submitted to T2 performed similar (P>0.05) to control in the final stage, whilst birds in T3 was the poorest (P<0.05). Furthermore, birds subjected to T2 showed no differences of edible carcass portions to the control, however, T3 group showed lower (P<0.05) slaughter and wing weights. Breast ultimate pH values of birds under T2 were similar to control, but birds in T3 had significantly higher (P<0.05) values. Birds under T2 had less (P<0.05) reddish breast meat colour than control at 45 minutes. At 24 hours post-mortem, birds in T3 had lower (P<0.05) lightness values and birds submitted to T2 had higher (P<0.05) yellowness values. Liver weight was significantly lower in birds under T3 and higher (P<0.05) gizzard weights were found in birds subjected to T2. Birds under T2 performed similar to control and were able to compensate for the weight loss when high plane of feed was re-introduced. It can be concluded from the results that restricting 15 percent of feed from Cobb 500 standard feed intake had moderately affected broiler performance. Moreover, restricting 15percent of feed significantly reduced feed intake in broiler chicken.
- Full Text:
The impact of rangeland degradation on vegetation cover and soil quality in semi-arid communal rangelands of the Eastern Cape province, South Africa
- Authors: Maziko, Yonela
- Date: 2017
- Subjects: Communal rangelands -- South Africa -- Eastern Cape Land degradation -- South Africa -- Eastern Cape Vegetation management -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/15056 , vital:40159
- Description: This study was conducted to investigate the influence of degradation on vegetation and soil quality in two semi-arid rangelands in the Eastern Cape Province of South Africa. The study was undertaken in Kwezana and Esigingqini communal areas that showed signs id degradation. The assessment incorporated the soil and grass layer in different degradation levels, namely nondegraded, slightly degraded, moderately and severely degraded. The non-degraded has a sufficient amount of vegetation cover and species composition. The slightly degraded site consists of a few visible bare patches. The moderately degraded areas showed signs of active sheet erosion such as pedestals and a higher percentage of bare areas and the severely degraded area is characterized by dongas and gullies, with vast areas that have no ground cover or top fertile soil layer. Using a step point method, herbaceous species composition was determined within three transects from three plots 100 m x 40 m in each degradation level. The aboveground phytomass production was determined by harvesting herbaceous species within four randomly placed 0.25 m2 quadrats in each transect. Bare ground was determined by counting and recording the distance between tufts within each quadrat. Litter cover was determined by visual observation and rated on a scale of 1 to 5. A number of physical and chemical soil properties were used to quantify the effects of degradation on soil quality. To determine soil chemical properties, three soil samples were collected at a depth of 20 cm, in each plot and analyzed for pH, soil macro (N, C, K, P, Ca, Mg) and micro (Zn, Fe, Mn and Cu) nutrients. Soil compaction was determined using a soil pocket penetrometer. Readings were taken at 5 mm from 30 points placed at 1 m intervals and distributed in a randomly established straight line in each plot. Soil loss was determined using a rainfall simulator. Splash cups were filled with soil and placed under the rainfall simulator for 8 minutes per rainstorm at 360 mm-1 intensity. Results showed that at Esigingqini a total of 20 herbaceous species were identified, 17 of which were grasses. About 60percent of the identified grasses were Increaser II species, 15percent were Increaser II and 25percent Decreasers. In terms of palatability, 50percent of the grass species had poor palatability and the remaining had either average or high palatability. At Kwezana, 18 herbaceous species were identified of which 28percent were Decreasers and 61percent were Increaser II species. Half of the species had low palatability and only 28percent had high palatability. In both communal areas, litter cover was highest (P < 0.05) in the non-degraded areas followed by the slightly degraded sites and this was to be expected. Similarly, in both communal areas, there was a shift in species composition of decreasers and perennial grasses being replaced by annual species of poor quality and low palatability as the degradation levels increased. Results on soil chemical properties showed that the non-degraded areas had higher (P < 0.05) pH levels (8.5) than the severely degraded soils (5). A similar trend following a degradation gradient was observed for some soil nutrients such as N, P or Mg. Both the macro and micro nutrient concentrations were relatively higher (P < 0.05) in summer than in winter in both communal areas. Results on physical properties showed that soil loss and compaction were relatively lower (P <0.05) in the slightly degraded and non-degraded sites and increased with severity of degradation. In both communal areas, the severely degraded areas lost soil up to 1.1 t ha-1 due to degradation and the non-degraded areas had a soil loss of less than 0.8 t ha-1. The rate of soil loss between the severely and non-degraded areas is significantly different. The differences may be attributed to the differences in the vegetation cover which influences the rate of erosion on the various degradation gradients. These results suggest that both the grass layer and soil quality is negatively affected by degradation. Therefore, there is a need for land use practices that promote the sustainable use of rangeland resources. Such practices may include rotational grazing, reseeding, veld resting and fire. Measures to control the consequences of degradation and rehabilitate these deteriorated rangelands must be a priority.
- Full Text:
- Authors: Maziko, Yonela
- Date: 2017
- Subjects: Communal rangelands -- South Africa -- Eastern Cape Land degradation -- South Africa -- Eastern Cape Vegetation management -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/15056 , vital:40159
- Description: This study was conducted to investigate the influence of degradation on vegetation and soil quality in two semi-arid rangelands in the Eastern Cape Province of South Africa. The study was undertaken in Kwezana and Esigingqini communal areas that showed signs id degradation. The assessment incorporated the soil and grass layer in different degradation levels, namely nondegraded, slightly degraded, moderately and severely degraded. The non-degraded has a sufficient amount of vegetation cover and species composition. The slightly degraded site consists of a few visible bare patches. The moderately degraded areas showed signs of active sheet erosion such as pedestals and a higher percentage of bare areas and the severely degraded area is characterized by dongas and gullies, with vast areas that have no ground cover or top fertile soil layer. Using a step point method, herbaceous species composition was determined within three transects from three plots 100 m x 40 m in each degradation level. The aboveground phytomass production was determined by harvesting herbaceous species within four randomly placed 0.25 m2 quadrats in each transect. Bare ground was determined by counting and recording the distance between tufts within each quadrat. Litter cover was determined by visual observation and rated on a scale of 1 to 5. A number of physical and chemical soil properties were used to quantify the effects of degradation on soil quality. To determine soil chemical properties, three soil samples were collected at a depth of 20 cm, in each plot and analyzed for pH, soil macro (N, C, K, P, Ca, Mg) and micro (Zn, Fe, Mn and Cu) nutrients. Soil compaction was determined using a soil pocket penetrometer. Readings were taken at 5 mm from 30 points placed at 1 m intervals and distributed in a randomly established straight line in each plot. Soil loss was determined using a rainfall simulator. Splash cups were filled with soil and placed under the rainfall simulator for 8 minutes per rainstorm at 360 mm-1 intensity. Results showed that at Esigingqini a total of 20 herbaceous species were identified, 17 of which were grasses. About 60percent of the identified grasses were Increaser II species, 15percent were Increaser II and 25percent Decreasers. In terms of palatability, 50percent of the grass species had poor palatability and the remaining had either average or high palatability. At Kwezana, 18 herbaceous species were identified of which 28percent were Decreasers and 61percent were Increaser II species. Half of the species had low palatability and only 28percent had high palatability. In both communal areas, litter cover was highest (P < 0.05) in the non-degraded areas followed by the slightly degraded sites and this was to be expected. Similarly, in both communal areas, there was a shift in species composition of decreasers and perennial grasses being replaced by annual species of poor quality and low palatability as the degradation levels increased. Results on soil chemical properties showed that the non-degraded areas had higher (P < 0.05) pH levels (8.5) than the severely degraded soils (5). A similar trend following a degradation gradient was observed for some soil nutrients such as N, P or Mg. Both the macro and micro nutrient concentrations were relatively higher (P < 0.05) in summer than in winter in both communal areas. Results on physical properties showed that soil loss and compaction were relatively lower (P <0.05) in the slightly degraded and non-degraded sites and increased with severity of degradation. In both communal areas, the severely degraded areas lost soil up to 1.1 t ha-1 due to degradation and the non-degraded areas had a soil loss of less than 0.8 t ha-1. The rate of soil loss between the severely and non-degraded areas is significantly different. The differences may be attributed to the differences in the vegetation cover which influences the rate of erosion on the various degradation gradients. These results suggest that both the grass layer and soil quality is negatively affected by degradation. Therefore, there is a need for land use practices that promote the sustainable use of rangeland resources. Such practices may include rotational grazing, reseeding, veld resting and fire. Measures to control the consequences of degradation and rehabilitate these deteriorated rangelands must be a priority.
- Full Text:
The impact of the farmer field school approach on small-scale vegetable farmers' knowledge, agency and production in Alice, Eastern Cape
- Authors: Apleni, Asanda
- Date: 2017
- Subjects: Agricultural extension work -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/8187 , vital:31873
- Description: This study investigates the impact of the Farmer Field School approach on the knowledge, agency and production of small-scale farmers. Farmer Field Schools were devised in the 1980s by the Food and Agriculture Organization as a form of adult education in agriculture. It is a group-based approach in which a facilitator meets with farmers on a regular basis and sets in motion a process by which farmers ‘learn how to learn’, both from themselves and from one another. However, it remains an open question whether the Farmer Field School approach could be a solution to South Africa’s abiding problem of weak agricultural extension. Early in 2015, the University of Fort Hare and the Nkonkobe Farmers’ Association initiated a number of study groups in the Alice area, based largely on Farmer Field School principles. The main objective of the study was to assess the impact of the Farmer Field School approach on small-scale farmers’ knowledge, agency and production, focusing on home gardeners. The study was conducted in the Raymond Mhlaba Local Municipality in Alice of the Eastern Cape Province of South Africa. The research consisted of a survey of all the study group participants involved with home gardens, including some who joined the groups in 2015 but left in 2016. Both cross-sectional and longitudinal designs were used. Findings from this study have shown how participation in the study groups modelled on the FFS approach can improve the knowledge and skills of home gardeners, while also enabling them to produce significantly larger quantities of vegetables, and even a marketable surplus, albeit from a small base. In order to make more people to join, more incentives should be made available. Such incentives should be made available only to “kick start” the FFS study groups and take heed in prevention of dependence.
- Full Text:
- Authors: Apleni, Asanda
- Date: 2017
- Subjects: Agricultural extension work -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/8187 , vital:31873
- Description: This study investigates the impact of the Farmer Field School approach on the knowledge, agency and production of small-scale farmers. Farmer Field Schools were devised in the 1980s by the Food and Agriculture Organization as a form of adult education in agriculture. It is a group-based approach in which a facilitator meets with farmers on a regular basis and sets in motion a process by which farmers ‘learn how to learn’, both from themselves and from one another. However, it remains an open question whether the Farmer Field School approach could be a solution to South Africa’s abiding problem of weak agricultural extension. Early in 2015, the University of Fort Hare and the Nkonkobe Farmers’ Association initiated a number of study groups in the Alice area, based largely on Farmer Field School principles. The main objective of the study was to assess the impact of the Farmer Field School approach on small-scale farmers’ knowledge, agency and production, focusing on home gardeners. The study was conducted in the Raymond Mhlaba Local Municipality in Alice of the Eastern Cape Province of South Africa. The research consisted of a survey of all the study group participants involved with home gardens, including some who joined the groups in 2015 but left in 2016. Both cross-sectional and longitudinal designs were used. Findings from this study have shown how participation in the study groups modelled on the FFS approach can improve the knowledge and skills of home gardeners, while also enabling them to produce significantly larger quantities of vegetables, and even a marketable surplus, albeit from a small base. In order to make more people to join, more incentives should be made available. Such incentives should be made available only to “kick start” the FFS study groups and take heed in prevention of dependence.
- Full Text:
The influence of extraction techniques on the chemical composition of essential oils from Pelargonium cv.rose in the Eastern Cape
- Authors: Ngcangatha-Maqhude, Bulelwa
- Date: 2017
- Subjects: Essences and essential oils Pelargoniums Rose geranium oil
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/8111 , vital:31524
- Description: Rose geranium is a world known essential oil plant priced for its high grade essential oil mostly utilized in the perfumery industry. However, South Africa has just joined the geranium oil production industry and still battling with the variations in oil yield and composition. Volatile components from Rose geranium were respectively extracted by three methods including hydro-distillation, steam distillation and solvent free microwave. The composition was characterized by GC-FID and GC-MS .No significant differences were found in the oil constituents, colour of the oil was the same in hydro-distillation and steam distillation (light green) but in solvent free microwave extraction the oil was clear because in the receiving flask there was hexane and the quantity of the oil was not the same. The difference was the time taken to extract the oil: Hydro-distillation took 3 hours, steam distillation took 1 hour, 30 minutes and solvent free microwave took only 30 minutes. The main constituents of the essential oil of rose geranium (steam distillation) are Dohne: Citronellol (20.61percent), geraniol (14.79percent), linalool (1.98percent), citronellyl formate (14.25percent), geranyl formate (10.25percent) and guaiadiene 6, 9 (7.22percent). Kieskammahoek: Citronellol (20.36percent), geraniol (9.81percent), linalool (1.54percent), citronellyl formate (16.09percent), geranyl formate F (8.96percent) and guaiadiene 6, 9 (8. 22percent).University of FortHare: Citronellol (21.55percent), geraniol (9.41percent), linalool (1.40percent), citronellyl formate (16.37percent), geranyl formate (8.10percent) and guaiadiene 6, 9 7.92percent). For hydro-distillation, Dohne: Citronellol (24.68percent), geraniol (11.68percent), linalool (11.74percent), citronellyl formate (14.97percent), geranyl formate (5.95percent) and guaiadiene 6, 9 (4.63percent). Kieskammahoek: Citronellol (25.25percent), geraniol (13.44percent), linalool (3.35percent), citronellyl formate (15.62percent), geranyl formate (5.40percent) and guaiadiene 6, 9(4.96percent).University of FortHare: Citronellol (24.73percent), geraniol (8.35percent), linalool (9.76percent), citronellyl formate (16.54percent), geranyl formate (4.42percent) and guaiadiene 6, 9(9.76percent). The solvent free microwave extraction , Dohne: Citronellol (20.87), geraniol (17.85percent), linalool (1.21percent), citronellyl formate (13.44percent), geranyl formate (10.26percent) and guaiadiene 6, 9 (4.59percent).Kieskammahoek: Citronellol (20.13percent), geraniol (5.40percent), linalool (0.28percent), citronellyl formate (14.22percent), geranyl formate (6.04percent) and guaiadiene 6, 9(8.63percent).University of FortHare: Citronellol (21.15percent), geraniol (22.36percent), linalool (1.37percent), citronellyl formate (12.13percent), geranyl formate (8.91percent) and guaiadiene 6, 9 (5.82percent).The percentage of oil composition in hydro-distillation and Solvent Free Microwave Extraction was higher than steam distillation.
- Full Text:
- Authors: Ngcangatha-Maqhude, Bulelwa
- Date: 2017
- Subjects: Essences and essential oils Pelargoniums Rose geranium oil
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/8111 , vital:31524
- Description: Rose geranium is a world known essential oil plant priced for its high grade essential oil mostly utilized in the perfumery industry. However, South Africa has just joined the geranium oil production industry and still battling with the variations in oil yield and composition. Volatile components from Rose geranium were respectively extracted by three methods including hydro-distillation, steam distillation and solvent free microwave. The composition was characterized by GC-FID and GC-MS .No significant differences were found in the oil constituents, colour of the oil was the same in hydro-distillation and steam distillation (light green) but in solvent free microwave extraction the oil was clear because in the receiving flask there was hexane and the quantity of the oil was not the same. The difference was the time taken to extract the oil: Hydro-distillation took 3 hours, steam distillation took 1 hour, 30 minutes and solvent free microwave took only 30 minutes. The main constituents of the essential oil of rose geranium (steam distillation) are Dohne: Citronellol (20.61percent), geraniol (14.79percent), linalool (1.98percent), citronellyl formate (14.25percent), geranyl formate (10.25percent) and guaiadiene 6, 9 (7.22percent). Kieskammahoek: Citronellol (20.36percent), geraniol (9.81percent), linalool (1.54percent), citronellyl formate (16.09percent), geranyl formate F (8.96percent) and guaiadiene 6, 9 (8. 22percent).University of FortHare: Citronellol (21.55percent), geraniol (9.41percent), linalool (1.40percent), citronellyl formate (16.37percent), geranyl formate (8.10percent) and guaiadiene 6, 9 7.92percent). For hydro-distillation, Dohne: Citronellol (24.68percent), geraniol (11.68percent), linalool (11.74percent), citronellyl formate (14.97percent), geranyl formate (5.95percent) and guaiadiene 6, 9 (4.63percent). Kieskammahoek: Citronellol (25.25percent), geraniol (13.44percent), linalool (3.35percent), citronellyl formate (15.62percent), geranyl formate (5.40percent) and guaiadiene 6, 9(4.96percent).University of FortHare: Citronellol (24.73percent), geraniol (8.35percent), linalool (9.76percent), citronellyl formate (16.54percent), geranyl formate (4.42percent) and guaiadiene 6, 9(9.76percent). The solvent free microwave extraction , Dohne: Citronellol (20.87), geraniol (17.85percent), linalool (1.21percent), citronellyl formate (13.44percent), geranyl formate (10.26percent) and guaiadiene 6, 9 (4.59percent).Kieskammahoek: Citronellol (20.13percent), geraniol (5.40percent), linalool (0.28percent), citronellyl formate (14.22percent), geranyl formate (6.04percent) and guaiadiene 6, 9(8.63percent).University of FortHare: Citronellol (21.15percent), geraniol (22.36percent), linalool (1.37percent), citronellyl formate (12.13percent), geranyl formate (8.91percent) and guaiadiene 6, 9 (5.82percent).The percentage of oil composition in hydro-distillation and Solvent Free Microwave Extraction was higher than steam distillation.
- Full Text:
The investigation of the role and the efficacy of learning technologies towards community skill development
- Authors: Masikisiki, Baphumelele
- Date: 2017
- Subjects: Web-based instruction Computer-assisted instruction
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/5972 , vital:29460
- Description: Research has revealed that during systems design and development of e-learning technologies there is a tendency of neglecting the needs of end users and focus on the design process and the technology factors, and this has traditionally been the reason for poor usability of otherwise well conceptualized systems, as a result a number of IT-based learning tools ended up not being usable and not being utilized effectively. This study aims to investigate the impact of e-learning technologies, how people perceive the usage of e-learning technologies towards community skill development. An evaluation of four different e-learning technologies was conducted to investigate the role and efficacy of e-learning technologies within the surrounding communities. Data was analyzed as nominal data using IBM Statistical Package for Social Sciences (SPSS) software 24. Descriptive analysis, frequency, reliability and correctional analysis and also measures of central tendency were computed. Reliability was evaluated for assessing the internal consistency of the items using Cronbach’s alpha. To analyze the relationship between variables matrices of Pearson’s correlation was used. Pearson’s correlation can only be accepted when the significant effect (P>.05), this indicates that there is a positive or a negative relationship between two variables, if these conditions are not met then the proposed correlation or hypothesis can be rejected. Results indicate a poor perception and poor acceptance of e-learning technologies due to a number of factors, these factors include lack of computer-self efficacy which leads to computer anxiety, affordability of internet connectivity which leads to inaccessible of e-learning technologies. The findings also indicated that LAMS was found to be less useable and less useful by a number of students. However, students who enjoy working in groups found LAMS to be useable because it was supporting their preferred learning style, whereas individualistic students preferred Moodle and Dokeos because it was supporting their personal preferences and assessment styles. Having understood all the characteristics of learning tools, relevant learning technologies that are suitable for students can then be recommended.
- Full Text:
- Authors: Masikisiki, Baphumelele
- Date: 2017
- Subjects: Web-based instruction Computer-assisted instruction
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/5972 , vital:29460
- Description: Research has revealed that during systems design and development of e-learning technologies there is a tendency of neglecting the needs of end users and focus on the design process and the technology factors, and this has traditionally been the reason for poor usability of otherwise well conceptualized systems, as a result a number of IT-based learning tools ended up not being usable and not being utilized effectively. This study aims to investigate the impact of e-learning technologies, how people perceive the usage of e-learning technologies towards community skill development. An evaluation of four different e-learning technologies was conducted to investigate the role and efficacy of e-learning technologies within the surrounding communities. Data was analyzed as nominal data using IBM Statistical Package for Social Sciences (SPSS) software 24. Descriptive analysis, frequency, reliability and correctional analysis and also measures of central tendency were computed. Reliability was evaluated for assessing the internal consistency of the items using Cronbach’s alpha. To analyze the relationship between variables matrices of Pearson’s correlation was used. Pearson’s correlation can only be accepted when the significant effect (P>.05), this indicates that there is a positive or a negative relationship between two variables, if these conditions are not met then the proposed correlation or hypothesis can be rejected. Results indicate a poor perception and poor acceptance of e-learning technologies due to a number of factors, these factors include lack of computer-self efficacy which leads to computer anxiety, affordability of internet connectivity which leads to inaccessible of e-learning technologies. The findings also indicated that LAMS was found to be less useable and less useful by a number of students. However, students who enjoy working in groups found LAMS to be useable because it was supporting their preferred learning style, whereas individualistic students preferred Moodle and Dokeos because it was supporting their personal preferences and assessment styles. Having understood all the characteristics of learning tools, relevant learning technologies that are suitable for students can then be recommended.
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The potential of Elephantorrhiza elephantina as an anthelmintic of goats in the Eastern Cape province, South Africa
- Authors: Mazhangara, Irene Rumbidza
- Date: 2017
- Subjects: Bioactive compounds -- South Africa -- Eastern Cape Medicinal plants -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/15068 , vital:40160
- Description: The main objective of the study was to investigate the potential of Elephantorrhiza elephantina in the control of gastro-intestinal worms in goats in the Eastern Cape Province, South Africa. The ethanolic, methanolic and aqueous phytochemical composition of E. elephantina root bark extracts were determined. The phytochemical analysis revealed a high concentration of alkaloids, condensed tannins, flavonoids, phenols and saponins in E. elephantina root extracts. Quantitative phytochemical analysis of alkaloids in water extract was 932.44 ± 1.23 mg. Condensed tannins, phenols and saponins in ethanol extract were 450.52 ± 4.56 mg CE/g, 476.11 ± 0.03 mg GAE/g and 832.85 ± 1.45 mg respectively. Flavonoids was 803.93 ± 0.02 mg QE/g in methanol extract as the highest recordings. In vitro screening of ethanol, methanol and water extracts of E. elephantina for potential anthelminthic activity against adult Paramphistomum cervi worm motility showed time and dose-dependent significant effects after 12 hours of treatment. Ethanolic extracts showed a highly significant (P < 0.05) inhibition of motility (86.67 and 96.67 percent) at concentrations 7.5 and 15 mg/ml, respectively. Methanolic and aqueous extracts exhibited a significant (P < 0.05) motility inhibition (96.67 and 66.67 percent, respectively) at concentration of 15 mg/ml 16 hours post-exposure. In vitro assessment of structural changes to adult gastro-intestinal worms following exposure to E. elephantina extracts was done using scanning electron microscopy (SEM). The SEM observations revealed structural changes in the worms after contact with E. elephantina extracts in comparison with the control worms kept in Phosphate-buffered saline (PBS). The major alterations were around the cuticle, posterior and anterior areas. These changes included longitudinal and transversal folds and thicker cuticula ridges, material aggregates around the posterior and anterior areas. The study showed that ethanol, methanol and water extracts of E. elephantina root contained significant amounts of alkaloids, condensed tannins, flavanoids, phenols and saponins. Validation studies showed that extracts of E. elephantina possess anthelminthic activities. The structural changes found in the worms exposed to extracts of E. elephantina root extracts affected their motility and nutrition, resulting in mortality.
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- Authors: Mazhangara, Irene Rumbidza
- Date: 2017
- Subjects: Bioactive compounds -- South Africa -- Eastern Cape Medicinal plants -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/15068 , vital:40160
- Description: The main objective of the study was to investigate the potential of Elephantorrhiza elephantina in the control of gastro-intestinal worms in goats in the Eastern Cape Province, South Africa. The ethanolic, methanolic and aqueous phytochemical composition of E. elephantina root bark extracts were determined. The phytochemical analysis revealed a high concentration of alkaloids, condensed tannins, flavonoids, phenols and saponins in E. elephantina root extracts. Quantitative phytochemical analysis of alkaloids in water extract was 932.44 ± 1.23 mg. Condensed tannins, phenols and saponins in ethanol extract were 450.52 ± 4.56 mg CE/g, 476.11 ± 0.03 mg GAE/g and 832.85 ± 1.45 mg respectively. Flavonoids was 803.93 ± 0.02 mg QE/g in methanol extract as the highest recordings. In vitro screening of ethanol, methanol and water extracts of E. elephantina for potential anthelminthic activity against adult Paramphistomum cervi worm motility showed time and dose-dependent significant effects after 12 hours of treatment. Ethanolic extracts showed a highly significant (P < 0.05) inhibition of motility (86.67 and 96.67 percent) at concentrations 7.5 and 15 mg/ml, respectively. Methanolic and aqueous extracts exhibited a significant (P < 0.05) motility inhibition (96.67 and 66.67 percent, respectively) at concentration of 15 mg/ml 16 hours post-exposure. In vitro assessment of structural changes to adult gastro-intestinal worms following exposure to E. elephantina extracts was done using scanning electron microscopy (SEM). The SEM observations revealed structural changes in the worms after contact with E. elephantina extracts in comparison with the control worms kept in Phosphate-buffered saline (PBS). The major alterations were around the cuticle, posterior and anterior areas. These changes included longitudinal and transversal folds and thicker cuticula ridges, material aggregates around the posterior and anterior areas. The study showed that ethanol, methanol and water extracts of E. elephantina root contained significant amounts of alkaloids, condensed tannins, flavanoids, phenols and saponins. Validation studies showed that extracts of E. elephantina possess anthelminthic activities. The structural changes found in the worms exposed to extracts of E. elephantina root extracts affected their motility and nutrition, resulting in mortality.
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