A comparative study of the in vitro antidiabetic properties, cytotoxicity and mechanism of action of Albuca bracteata and Albuca setosa bulb extracts
- Authors: Odeyemi, Samuel Wale
- Date: 2015
- Subjects: Medicinal plants Herbs -- Therapeutic use Diabetics -- Alternative treatment
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10353/3154 , vital:28327
- Description: The search for cheap, non toxic and readily available antidiabetic drugs has been a challenge for researchers and the pharmaceutical industries. Diabetes mellitus is a metabolic disease characterized by defects in the synthesis of insulin and/or insensitivity to the action of insulin at the target cells. The disease has been on the increase mostly in developing countries where large proportions of the population have little access to good medical care due to either accessibility or non availability of synthetic drugs. This has led to the use of medicinal plants to treat diabetes because it is safe, cheap and with few side effects. There is little scientific evidence on the dosages, active compounds, mechanisms of action and toxicity of these traditionally used plants. Two of the most frequently used plants; Albuca setosa and Albuca bracteata were investigated in this study. The qualitative analysis of different extractions of these plants revealed the presence of phenolics, alkaloids, tannins and saponins. The antioxidant properties of aqueous, acetone and methanollic extracts of Albuca setosa and Albuca bracteata were investigated using models such as Diphenyl-1-Picrylhydrazyl (DPPH), 2, 2’-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), Ferric ion reducing antioxidant potential (FRAP), Nitric Oxide and Hydrogen Peroxide (H2O2). Both plants revealed inhibitions against DPPH in a concentration - dependent manner with Albuca setosa (0.330 mg/ml) showing higher activity than Albuca bracteata (0.647 mg/ml) determined from the IC50. The aqueous extract of Albuca setosa showed a higher inhibition against DPPH radical compared to the Albuca bracteata aqueous extract at all concentrations investigated. The isolated saponins from Albuca bracteata had a higher DPPH scavenging activity than the crude methanolic extract of the plant in a concentration - dependent manner but are significantly different from each other at 0.4, 0.6 and 1.0 mg/ml only. The IC50 of the saponins was also observed to be higher than the crude extracts and standards.The Albuca setosa aqueous extract showed a higher percentage inhibition of ABTS radicals than Albuca bracteata at all the concentrations investigated. Overall, the Albuca setosa aqueous extract (0.0809 mg/ml) showed maximum activity against ABTS radicals. The iron reducing power was significantly higher (P < 0.05) in the methanolic extract of both plants compared to the aqueous counterpart. Overall, the Albuca bracteata aqueous extract (0.344 mg/ml) showed maximum activity as indicated by the IC50. The aqueous extracts of both plants also revealed percentage inhibitions in a concentration - dependent manner against NO2. The aqueous extract of Albuca bracteata bulb was more active against nitric oxide and hydrogen peroxide inhibition. In this study, the cytotoxicity of the extracts was evaluated at a high dose of 100 μg/ml on Chang liver cells and determined using MTT, crystal violet, glucose consumption, lactate production and lactate dehydrogenase release and FRAP. The aqueous extracts of both Albuca setosa and Albuca bracteata were non-toxic on Chang liver cells at the concentrations investigated. The MTT revealed that the aqueous extract of Albuca setosa bulb had the optimum cell viability of 108.09 percent while the acetonic extract of Albuca bracteata showed the least cell viability (37.72 percent) compared with the control. The crystal violet test also revealed the acetone extract of Albuca bracteata to have the least percentage of cell viability at 31.47 percent, while the aqueous extract of Albuca setosa showed the maximum cell viability at 112.5 percent. The aqueous extracts of both plants showed higher percentage cell density on the second day of incubation from the proliferation assay. All the tested samples were observed to consume more glucose than the blank except for the methanollic and acetone extracts of Albuca bracteata bulb. The aqueous and methanolic extracts of Albuca setosa bulbs produced the highest lactate with 120.2 μg/ml and 113.7 μg/ml respectively. The acetone extracts of both Albuca setosa and Albuca bracteata revealed toxicity with a higher lactate dehydrogenase release compared to the control.
- Full Text:
- Date Issued: 2015
- Authors: Odeyemi, Samuel Wale
- Date: 2015
- Subjects: Medicinal plants Herbs -- Therapeutic use Diabetics -- Alternative treatment
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10353/3154 , vital:28327
- Description: The search for cheap, non toxic and readily available antidiabetic drugs has been a challenge for researchers and the pharmaceutical industries. Diabetes mellitus is a metabolic disease characterized by defects in the synthesis of insulin and/or insensitivity to the action of insulin at the target cells. The disease has been on the increase mostly in developing countries where large proportions of the population have little access to good medical care due to either accessibility or non availability of synthetic drugs. This has led to the use of medicinal plants to treat diabetes because it is safe, cheap and with few side effects. There is little scientific evidence on the dosages, active compounds, mechanisms of action and toxicity of these traditionally used plants. Two of the most frequently used plants; Albuca setosa and Albuca bracteata were investigated in this study. The qualitative analysis of different extractions of these plants revealed the presence of phenolics, alkaloids, tannins and saponins. The antioxidant properties of aqueous, acetone and methanollic extracts of Albuca setosa and Albuca bracteata were investigated using models such as Diphenyl-1-Picrylhydrazyl (DPPH), 2, 2’-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), Ferric ion reducing antioxidant potential (FRAP), Nitric Oxide and Hydrogen Peroxide (H2O2). Both plants revealed inhibitions against DPPH in a concentration - dependent manner with Albuca setosa (0.330 mg/ml) showing higher activity than Albuca bracteata (0.647 mg/ml) determined from the IC50. The aqueous extract of Albuca setosa showed a higher inhibition against DPPH radical compared to the Albuca bracteata aqueous extract at all concentrations investigated. The isolated saponins from Albuca bracteata had a higher DPPH scavenging activity than the crude methanolic extract of the plant in a concentration - dependent manner but are significantly different from each other at 0.4, 0.6 and 1.0 mg/ml only. The IC50 of the saponins was also observed to be higher than the crude extracts and standards.The Albuca setosa aqueous extract showed a higher percentage inhibition of ABTS radicals than Albuca bracteata at all the concentrations investigated. Overall, the Albuca setosa aqueous extract (0.0809 mg/ml) showed maximum activity against ABTS radicals. The iron reducing power was significantly higher (P < 0.05) in the methanolic extract of both plants compared to the aqueous counterpart. Overall, the Albuca bracteata aqueous extract (0.344 mg/ml) showed maximum activity as indicated by the IC50. The aqueous extracts of both plants also revealed percentage inhibitions in a concentration - dependent manner against NO2. The aqueous extract of Albuca bracteata bulb was more active against nitric oxide and hydrogen peroxide inhibition. In this study, the cytotoxicity of the extracts was evaluated at a high dose of 100 μg/ml on Chang liver cells and determined using MTT, crystal violet, glucose consumption, lactate production and lactate dehydrogenase release and FRAP. The aqueous extracts of both Albuca setosa and Albuca bracteata were non-toxic on Chang liver cells at the concentrations investigated. The MTT revealed that the aqueous extract of Albuca setosa bulb had the optimum cell viability of 108.09 percent while the acetonic extract of Albuca bracteata showed the least cell viability (37.72 percent) compared with the control. The crystal violet test also revealed the acetone extract of Albuca bracteata to have the least percentage of cell viability at 31.47 percent, while the aqueous extract of Albuca setosa showed the maximum cell viability at 112.5 percent. The aqueous extracts of both plants showed higher percentage cell density on the second day of incubation from the proliferation assay. All the tested samples were observed to consume more glucose than the blank except for the methanollic and acetone extracts of Albuca bracteata bulb. The aqueous and methanolic extracts of Albuca setosa bulbs produced the highest lactate with 120.2 μg/ml and 113.7 μg/ml respectively. The acetone extracts of both Albuca setosa and Albuca bracteata revealed toxicity with a higher lactate dehydrogenase release compared to the control.
- Full Text:
- Date Issued: 2015
Identification and documentation of ethnobiological methods used by rural farmers to control stalk borers on maize in the Eastern Cape Province of South Africa
- Authors: Skenjana, Nolitha Leonora
- Date: 2015
- Subjects: Ethnobotany , Stem borers , Biological pest control agents -- South Africa , Corn -- Diseases and pests -- South Africa
- Language: English
- Type: Thesis , Masters , MSc (Ethnobotany)
- Identifier: vital:11312 , http://hdl.handle.net/10353/d1019852 , Ethnobotany , Stem borers , Biological pest control agents -- South Africa , Corn -- Diseases and pests -- South Africa
- Description: Maize contributes substantially to food security in the Eastern Cape province of South Africa. It is a staple food to many of the province’s rural and urban inhabitants. Insect pests are one of the factors that hamper its productivity and as a result, deprive farmers of good yields. The adverse effects of insecticides and the high cost associated with them and the cost of transgenic seeds are some of the challenges faced by small-scale farmers in rural areas. Alternative control methods which include the use of indigenous techniques to control pests are now sought. A study to identify and document ethnobiological means used by rural farmers to manage insect pests of maize was conducted in the rural areas of the Eastern Cape Province. A total of 217 participants were interviewed on the matter, using semi-structured but detailed questionnaires. Rural farmers due to their linkage to agriculture activities and the fact that they are considered as custodians of agricultural indigenous knowledge were selected as respondents. Only maize producing and IsiXhosa speaking people were chosen to contribute. Main focus was on the demography of respondents, crop production activities and insect pest control. Pretesting of the questionnaire in order to assess the appropriateness of questions and comprehension by both farmers and enumerators was done. Data was analysed using descriptive statistics. Fifty five percent (55 %) of the respondents were females and the highest number of participants was from the Chris Hani District Municipality. Majority of the people were unemployed or pensioners. Most had only attended primary school and the mean age was 59 years. Apart from maize, respondents were cultivating other crops such as cabbage, Swiss chard, potatoes etc. Stalk borers followed by cutworms were the main pests of maize in these areas. Respondents used mainly insecticides, followed by alternative substances, which also included cultural control methods such as planting date manipulation. Few respondents used plants as control agents for insect pests. Some people did not control pests even though they were a problem in their fields. The most used plant was Chenopodium ambrosiodes L, while the most used substance was Madubula (a detergent). The most used insecticide was carbaryl from the carbamite family. Respondents listed different preparation techniques for all the control methods mentioned. These techniques revealed different times of preparation, quantities of ingredients, amounts applied on plants, modes of application and intervals of application. Rural farmers in the study areas used different atypical methods which may play a significant role in pest management today. Some of the products may have a positive influence on agriculture, while some are dangerous to humans and environmental health. Further research which will investigate their potential use in pest control needs to be done.
- Full Text:
- Date Issued: 2015
- Authors: Skenjana, Nolitha Leonora
- Date: 2015
- Subjects: Ethnobotany , Stem borers , Biological pest control agents -- South Africa , Corn -- Diseases and pests -- South Africa
- Language: English
- Type: Thesis , Masters , MSc (Ethnobotany)
- Identifier: vital:11312 , http://hdl.handle.net/10353/d1019852 , Ethnobotany , Stem borers , Biological pest control agents -- South Africa , Corn -- Diseases and pests -- South Africa
- Description: Maize contributes substantially to food security in the Eastern Cape province of South Africa. It is a staple food to many of the province’s rural and urban inhabitants. Insect pests are one of the factors that hamper its productivity and as a result, deprive farmers of good yields. The adverse effects of insecticides and the high cost associated with them and the cost of transgenic seeds are some of the challenges faced by small-scale farmers in rural areas. Alternative control methods which include the use of indigenous techniques to control pests are now sought. A study to identify and document ethnobiological means used by rural farmers to manage insect pests of maize was conducted in the rural areas of the Eastern Cape Province. A total of 217 participants were interviewed on the matter, using semi-structured but detailed questionnaires. Rural farmers due to their linkage to agriculture activities and the fact that they are considered as custodians of agricultural indigenous knowledge were selected as respondents. Only maize producing and IsiXhosa speaking people were chosen to contribute. Main focus was on the demography of respondents, crop production activities and insect pest control. Pretesting of the questionnaire in order to assess the appropriateness of questions and comprehension by both farmers and enumerators was done. Data was analysed using descriptive statistics. Fifty five percent (55 %) of the respondents were females and the highest number of participants was from the Chris Hani District Municipality. Majority of the people were unemployed or pensioners. Most had only attended primary school and the mean age was 59 years. Apart from maize, respondents were cultivating other crops such as cabbage, Swiss chard, potatoes etc. Stalk borers followed by cutworms were the main pests of maize in these areas. Respondents used mainly insecticides, followed by alternative substances, which also included cultural control methods such as planting date manipulation. Few respondents used plants as control agents for insect pests. Some people did not control pests even though they were a problem in their fields. The most used plant was Chenopodium ambrosiodes L, while the most used substance was Madubula (a detergent). The most used insecticide was carbaryl from the carbamite family. Respondents listed different preparation techniques for all the control methods mentioned. These techniques revealed different times of preparation, quantities of ingredients, amounts applied on plants, modes of application and intervals of application. Rural farmers in the study areas used different atypical methods which may play a significant role in pest management today. Some of the products may have a positive influence on agriculture, while some are dangerous to humans and environmental health. Further research which will investigate their potential use in pest control needs to be done.
- Full Text:
- Date Issued: 2015
Nutritional value and cultivation requirements of Cleome Gynandra: a wild vegetable growing in the Eastern Cape Province, South Africa
- Sowunmi, Linda Ifeanyichukwu
- Authors: Sowunmi, Linda Ifeanyichukwu
- Date: 2015
- Subjects: Ethnobotany -- South Africa -- Eastern Cape Vegetables -- South Africa -- Eastern Cape Nutrition -- South Africa -- Evaluation
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10353/2600 , vital:27907
- Description: South Africa has wide ranges of plants among which are leafy vegetables growing in the wild. These wild vegetables have been reported to have high nutritive and medicinal potentials. Yet, there is still high prevalence of malnutrition and micronutrients deficiencies among the low income bracket of the population. Effective utilization of wild vegetables has been proposed as part of the solutions to address the problem of dietary deficiencies among the populace. Their importance is mainly as relish to accompany and complement starch based diets. They are also known to serve as supplements for food which have the potential to improve the health status of its consumers in many rural communities. Cleome gynandra L. (Capparaceae) is one of the underutilized species mentioned during asurvey conducted in Nkonkobe municipality of the Eastern Cape Province, South Africa. The plant is an erect annual herb with alternate, palmately compound leaves and its petals are white, pink or lilac. It grows as a weed in this part of the Province and is usually gathered from the wild for food and medicine. It is commonly found on wastes land, road sides and on grass lands. Therefore, this research work was designed to contribute to the possible domestication of this wild vegetable in order to explore the nutritive and therapeutic potentials which would broaden the food base in the Eastern Cape Province. The ultra-micromorphological features of plants have become an essential tool in proper identification and authentication of several plant species. In the light of this, the micromorphology of Cleome gynandra was examined to reveal the micro-morphological characters of the plantand to determine if micro and macro mineral elements are present using energy dispersive x-rays. The present study revealed the epidermal cells of the leaves to be asymmetric in shape with undulating cell walls having four subsidiary cells around the stomata. The leaves have anomocyctic stomata which are more distributed in the abaxial surface than the adaxial surface. The mean length and width of the guard cells in the abaxial surface are 0.09 ± 0.01 mm and 0.08 ± 0.01 mm respectively while that of adaxial surface are 0.1± 0.01 mm and 0.07 ± 0.01 mm respectively. The energy dispersive x-rays (EDXS) micro-analysis of the leaf revealed the presence of phosphorus, manganese, iron, calcium, sodium, magnesium, potassium and zinc as the major constituents of the crystal deposits present in the stomata pores and the mesophyll. C. gynandra was collected from the wild and analysed for its mineral and proximatecomposition using standard analytical methods. The plant showed high levels of sodium, iron, zinc, calcium and potassium while many other macro and micro minerals were moderately present. Amongst the proximate factors investigated, moisture content was found to be 82.1% while crude fibre and protein contents were 39.9 percent and 31.03 percent respectively. Vitamin C was also high (345.3 mg/100g). The anti-nutrients compositions revealed low concentrations of oxalate (7.4 ± 0.4) and phytate (0.6 ± 0.0) mg/100g. The study revealed high nutritive value of the species growing naturally from the wild. Interest in the phenolic compounds has greatly increased recently because these compounds have been implicated in suppressing the risk of degenerative diseases in humans. The pharmacological properties of plants may be related to their antioxidant capacities and hence there was need to investigate the antioxidant potential using aqueous, ethanolic and acetone extracts of different parts of C. gynandra. The quantities of phenols, flavonoids and flavonols were significantly higher (p < 0.05) in both aqueous and ethanol extracts of the leaf while the concentration of proanthocyanidins was higher in the aqueous stem extract compared to other plant parts. The aqueous extract of the different parts exhibited better ABTS, reducing power and NO radical scavenging abilities than ethanol extract.
- Full Text:
- Date Issued: 2015
- Authors: Sowunmi, Linda Ifeanyichukwu
- Date: 2015
- Subjects: Ethnobotany -- South Africa -- Eastern Cape Vegetables -- South Africa -- Eastern Cape Nutrition -- South Africa -- Evaluation
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10353/2600 , vital:27907
- Description: South Africa has wide ranges of plants among which are leafy vegetables growing in the wild. These wild vegetables have been reported to have high nutritive and medicinal potentials. Yet, there is still high prevalence of malnutrition and micronutrients deficiencies among the low income bracket of the population. Effective utilization of wild vegetables has been proposed as part of the solutions to address the problem of dietary deficiencies among the populace. Their importance is mainly as relish to accompany and complement starch based diets. They are also known to serve as supplements for food which have the potential to improve the health status of its consumers in many rural communities. Cleome gynandra L. (Capparaceae) is one of the underutilized species mentioned during asurvey conducted in Nkonkobe municipality of the Eastern Cape Province, South Africa. The plant is an erect annual herb with alternate, palmately compound leaves and its petals are white, pink or lilac. It grows as a weed in this part of the Province and is usually gathered from the wild for food and medicine. It is commonly found on wastes land, road sides and on grass lands. Therefore, this research work was designed to contribute to the possible domestication of this wild vegetable in order to explore the nutritive and therapeutic potentials which would broaden the food base in the Eastern Cape Province. The ultra-micromorphological features of plants have become an essential tool in proper identification and authentication of several plant species. In the light of this, the micromorphology of Cleome gynandra was examined to reveal the micro-morphological characters of the plantand to determine if micro and macro mineral elements are present using energy dispersive x-rays. The present study revealed the epidermal cells of the leaves to be asymmetric in shape with undulating cell walls having four subsidiary cells around the stomata. The leaves have anomocyctic stomata which are more distributed in the abaxial surface than the adaxial surface. The mean length and width of the guard cells in the abaxial surface are 0.09 ± 0.01 mm and 0.08 ± 0.01 mm respectively while that of adaxial surface are 0.1± 0.01 mm and 0.07 ± 0.01 mm respectively. The energy dispersive x-rays (EDXS) micro-analysis of the leaf revealed the presence of phosphorus, manganese, iron, calcium, sodium, magnesium, potassium and zinc as the major constituents of the crystal deposits present in the stomata pores and the mesophyll. C. gynandra was collected from the wild and analysed for its mineral and proximatecomposition using standard analytical methods. The plant showed high levels of sodium, iron, zinc, calcium and potassium while many other macro and micro minerals were moderately present. Amongst the proximate factors investigated, moisture content was found to be 82.1% while crude fibre and protein contents were 39.9 percent and 31.03 percent respectively. Vitamin C was also high (345.3 mg/100g). The anti-nutrients compositions revealed low concentrations of oxalate (7.4 ± 0.4) and phytate (0.6 ± 0.0) mg/100g. The study revealed high nutritive value of the species growing naturally from the wild. Interest in the phenolic compounds has greatly increased recently because these compounds have been implicated in suppressing the risk of degenerative diseases in humans. The pharmacological properties of plants may be related to their antioxidant capacities and hence there was need to investigate the antioxidant potential using aqueous, ethanolic and acetone extracts of different parts of C. gynandra. The quantities of phenols, flavonoids and flavonols were significantly higher (p < 0.05) in both aqueous and ethanol extracts of the leaf while the concentration of proanthocyanidins was higher in the aqueous stem extract compared to other plant parts. The aqueous extract of the different parts exhibited better ABTS, reducing power and NO radical scavenging abilities than ethanol extract.
- Full Text:
- Date Issued: 2015
Phytochemical analysis and antibacterial properties of aqueous and ethanol extracts of Brachylaena elliptica (Thurb.) dc. and Brachylaena ilicifolia (Lam.) Phill & Schweick
- Authors: Sagbo, Idowu Jonas
- Date: 2015
- Subjects: Medicinal plants , Traditional medicine , Herbs -- Therapeutic use
- Language: English
- Type: Thesis , Masters , MSc (Biochemistry)
- Identifier: vital:11297 , http://hdl.handle.net/10353/d1021289 , Medicinal plants , Traditional medicine , Herbs -- Therapeutic use
- Description: Resistance of human pathogenic bacterial strains results in selective pressure against known antibiotic. However, plant derived compounds that possess antibacterial potential are currently being investigated for treatment of wound infections in diabetic patients as they are inexpensive and non-toxic. Hence, this dissertation was designed to evaluate two medicinal plants (Brachylaena elliptica and Brachylaena ilicifolia) traditionally used in the treatment of various diseases such as diabetes, and its secondary complications in diabetic patients. The in vitro antioxidant activity of both plants were evaluated using DPPH (1, 1-diphenylhydrazl), ferric reducing power, ABTS (2, 2’-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid), NO (nitric oxide) and H2O2 (hydrogen peroxide) techniques. The antibacterial test and Minimum inhibitory concentration (MIC) was determined by agar dilution method against 5 bacteria strains (Pseudomonas aeruginosa, Staphylococcus aureus, Streptococcus pyogene, Proteus vulgaris and Proteus mirabilis) infecting wounds in diabetic patients using amoxicillin and ciprofloxacin as positive control. The phytochemical analyses were assessed using standard published methods. Identification of bioactive components in essential oils of both plants were assessed using GCMS. The aqueous and ethanol extracts of both plants were also evaluated to identify bioactive components using LC-MS. The results of the phytochemical analysis revealed the presence of phenols, tannins, flavanoids, flavanols, proanthocyanidins, saponins and alkaloids in both plants. Both plants indicated strong antioxidant activities which might be due to the presence of bioactive compounds. The aqueous and ethanol leaf extracts of both plants demonstrated appreciable broad spectrum activities against these wound pathogens with MIC ranging between 5 and 0.3 mg/ml. The GC-MS analysis of the essential oils of both plants revealed the presence of monoterpenes, oxygenated sesquiterpenes, phenolics and esters. The LC-MS analysis of the aqueous and ethanol leaf extracts of both plants showed that both plants are rich in alkaloids, terpenes, terpenoids, monoterpernoids, and flavanoids. Conclusively, this study has partially justified the ethnomedicinal use of B. elliptica and B.licifolia leaves for the treatment of various diseases, including diabetes and wound infections caused by bacteria in diabetic patients. These may be attributed to the presence of antioxidant compound such as phenols, flavanoids, saponins, tannins, alkaloids and other phytochemical compounds.
- Full Text:
- Date Issued: 2015
- Authors: Sagbo, Idowu Jonas
- Date: 2015
- Subjects: Medicinal plants , Traditional medicine , Herbs -- Therapeutic use
- Language: English
- Type: Thesis , Masters , MSc (Biochemistry)
- Identifier: vital:11297 , http://hdl.handle.net/10353/d1021289 , Medicinal plants , Traditional medicine , Herbs -- Therapeutic use
- Description: Resistance of human pathogenic bacterial strains results in selective pressure against known antibiotic. However, plant derived compounds that possess antibacterial potential are currently being investigated for treatment of wound infections in diabetic patients as they are inexpensive and non-toxic. Hence, this dissertation was designed to evaluate two medicinal plants (Brachylaena elliptica and Brachylaena ilicifolia) traditionally used in the treatment of various diseases such as diabetes, and its secondary complications in diabetic patients. The in vitro antioxidant activity of both plants were evaluated using DPPH (1, 1-diphenylhydrazl), ferric reducing power, ABTS (2, 2’-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid), NO (nitric oxide) and H2O2 (hydrogen peroxide) techniques. The antibacterial test and Minimum inhibitory concentration (MIC) was determined by agar dilution method against 5 bacteria strains (Pseudomonas aeruginosa, Staphylococcus aureus, Streptococcus pyogene, Proteus vulgaris and Proteus mirabilis) infecting wounds in diabetic patients using amoxicillin and ciprofloxacin as positive control. The phytochemical analyses were assessed using standard published methods. Identification of bioactive components in essential oils of both plants were assessed using GCMS. The aqueous and ethanol extracts of both plants were also evaluated to identify bioactive components using LC-MS. The results of the phytochemical analysis revealed the presence of phenols, tannins, flavanoids, flavanols, proanthocyanidins, saponins and alkaloids in both plants. Both plants indicated strong antioxidant activities which might be due to the presence of bioactive compounds. The aqueous and ethanol leaf extracts of both plants demonstrated appreciable broad spectrum activities against these wound pathogens with MIC ranging between 5 and 0.3 mg/ml. The GC-MS analysis of the essential oils of both plants revealed the presence of monoterpenes, oxygenated sesquiterpenes, phenolics and esters. The LC-MS analysis of the aqueous and ethanol leaf extracts of both plants showed that both plants are rich in alkaloids, terpenes, terpenoids, monoterpernoids, and flavanoids. Conclusively, this study has partially justified the ethnomedicinal use of B. elliptica and B.licifolia leaves for the treatment of various diseases, including diabetes and wound infections caused by bacteria in diabetic patients. These may be attributed to the presence of antioxidant compound such as phenols, flavanoids, saponins, tannins, alkaloids and other phytochemical compounds.
- Full Text:
- Date Issued: 2015
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