Assessment of phytochemical constituents, antioxidant properties and cytotoxicity potentials of Leonutis leonurus and Boophone disticha extracts used in the management of Alzheimer’s disease in Hogsback, Eastern Cape, South Africa
- Authors: Tonisi, Sipho
- Date: 2019
- Subjects: Medicinal plants Alzheimer's disease
- Language: English
- Type: Thesis , Masters , MSc (Microbiology)
- Identifier: http://hdl.handle.net/10353/14726 , vital:40067
- Description: In this present study, the phytochemical compounds, antioxidant activities, and cytotoxicity potentials of solvent extracts (polar solvents) of Leonotis leonurus (L. leonurus) and Boophone disitcha (B. disticha) commonly used in the management of Alzheimer’s diseases in Hogsback, in the Eastern Cape, South Africa were investigated. The phytochemical compounds were determined by standard protocols of Folin–Ciocalteu method, aluminium chloride colorimetric method as well as Mayer’s and Wagner’s method. The antioxidant capabilities were assessed using 2,2-diphenyl-1-picrylhydrazyl (DPPH), hydrogen peroxide, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) and nitric oxide radical scavenging assays. The majority of extracts from both plant species exhibited free radical scavenging potential for oxidants they were tested against viz; DPPH, hydrogen peroxide, ABTS and nitric oxide. They further displayed good reducing potential against ferric iron. The results have shown a concentration-dependent dose of inhibition/scavenging activity (in DPPH, hydrogen peroxide, ABTS and nitric oxide assays) and reducing ability (in ferric reducing assay). However, leaf extracts of L. leonurus displayed ineffective scavenging activity against nitric oxide whereas extracts from L. leonurus flower and B. disticha bulb had higher scavenging potential against the production of nitric oxide radical. The GC-MS profiles of the plants extracts revealed the presence of numerous bioactive compounds that are known to possess excellent anti-inflammatory and most importantly antioxidant properties. Another interesting observation of GC-MS analysis data was the presence of compounds reported to inhibit Acetycholinesterase (AChE), which is a key enzyme targeted in the treatment of Alzheimers’ Disease (AD). The cytotoxicy tests for these extracts were carried out in order to assess the potentential detrimental effects on human health using Alamar Blue assay. It was noted that an increase in the extract concentration of both plant species resulted in a noticeable decline in cell population. These extracts were then declared xix to be quite toxic against HeLa (human cervix adenocarcinoma cells) cells as the cell viability in some extracts was quite low. Hence, toxicity testing of the studied plants’ extracts against normal cells is highly imperative in the future study. The results from this present study scientifically validated the use of the studied plants in folkoric medicine for the management of Alzheimer’s diseases.
- Full Text:
- Authors: Tonisi, Sipho
- Date: 2019
- Subjects: Medicinal plants Alzheimer's disease
- Language: English
- Type: Thesis , Masters , MSc (Microbiology)
- Identifier: http://hdl.handle.net/10353/14726 , vital:40067
- Description: In this present study, the phytochemical compounds, antioxidant activities, and cytotoxicity potentials of solvent extracts (polar solvents) of Leonotis leonurus (L. leonurus) and Boophone disitcha (B. disticha) commonly used in the management of Alzheimer’s diseases in Hogsback, in the Eastern Cape, South Africa were investigated. The phytochemical compounds were determined by standard protocols of Folin–Ciocalteu method, aluminium chloride colorimetric method as well as Mayer’s and Wagner’s method. The antioxidant capabilities were assessed using 2,2-diphenyl-1-picrylhydrazyl (DPPH), hydrogen peroxide, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) and nitric oxide radical scavenging assays. The majority of extracts from both plant species exhibited free radical scavenging potential for oxidants they were tested against viz; DPPH, hydrogen peroxide, ABTS and nitric oxide. They further displayed good reducing potential against ferric iron. The results have shown a concentration-dependent dose of inhibition/scavenging activity (in DPPH, hydrogen peroxide, ABTS and nitric oxide assays) and reducing ability (in ferric reducing assay). However, leaf extracts of L. leonurus displayed ineffective scavenging activity against nitric oxide whereas extracts from L. leonurus flower and B. disticha bulb had higher scavenging potential against the production of nitric oxide radical. The GC-MS profiles of the plants extracts revealed the presence of numerous bioactive compounds that are known to possess excellent anti-inflammatory and most importantly antioxidant properties. Another interesting observation of GC-MS analysis data was the presence of compounds reported to inhibit Acetycholinesterase (AChE), which is a key enzyme targeted in the treatment of Alzheimers’ Disease (AD). The cytotoxicy tests for these extracts were carried out in order to assess the potentential detrimental effects on human health using Alamar Blue assay. It was noted that an increase in the extract concentration of both plant species resulted in a noticeable decline in cell population. These extracts were then declared xix to be quite toxic against HeLa (human cervix adenocarcinoma cells) cells as the cell viability in some extracts was quite low. Hence, toxicity testing of the studied plants’ extracts against normal cells is highly imperative in the future study. The results from this present study scientifically validated the use of the studied plants in folkoric medicine for the management of Alzheimer’s diseases.
- Full Text:
Assessment of quality nursing care in healthcare facilities of Mnquma Sub-District
- Authors: Manyela, Nosiphiwo
- Date: 2019
- Subjects: Health facilities
- Language: English
- Type: Thesis , Masters , MPH
- Identifier: http://hdl.handle.net/10353/16747 , vital:40770
- Description: Aim: The purpose of the study was to assess the quality of patient care in the Primary Health Care (PHC) facilities of Mnquma Sub-district with the goal of improving or maintaining quality nursing in the area. Methodology: This study followed a quantitative research approach and it is descriptive in nature. The study involved 561 participants, of whom 406 participants were from urban clinics and 155 were from rural facilities, all these participants were attending clinics at Mnquma Sub-district. A structured questionnaire was used for data collection, since the participants were able to read and write in either English and or IsiXhosa. The data was analyzed with the help of a statistician using Statistical Package for Social Sciences (SPSS) program, software for windows version 21.0 Results: Results showed that participant’s responses to five of the twelve items on patient safety were found to have significant associations with residential setting. Rural participants were less likely to disagree with statements on availability of signage at the entrance of health establishment that indicates times when various services are offered, availability of the security guards to ensure patient safety, warning signs always put in place when the floors are wet, availability of at least one toilet for disabled patients in the health establishment and the fact that health professionals are always wearing name tags. This means that patient safety and security is practiced and implemented at the rural facilities according to the national core standards. The findings of the study confirmed that 13 items out of 19 under care and attitudes were found to have significant associations with residential setting. The items were patients spending less than 2 hours in the health facility; staff treating patients politely, illness being explained clearly to the patients, and ability of the patients to ask questions about their illness. Other items included nursing attitudes during opening and closing times of the health establishment, staff responding well when requested to assist, patients are being treated with empathy, patients are addressed by names and patients are being given all the treatment that they need, nurses explains the treatment side effects, and explains how to deal with side effects. This means that responses to these items depend on whether the patient is from a rural or urban clinic. Rural communities were found to be vi less likely to disagree with statements as compared to urban communities. This means that nurses are compliant with nursing care and attitudes in rural clinics. Findings of the study with respect to facility cleanliness, shows that there is no significant associations between the responses to the items which are: surroundings of the clinic are free of unpleasant smells, grounds of the clinic are clean, safe and clean grounds, bin waste inside and outside storage poses no health risks, and setting were detected. This means that the facilities are and cleanliness status remains the same across the residential settings. However, the younger age group was found be significantly more likely to disagree that toilets are clean and odorless and that grounds are safe and clean compared to the older age group. This suggests that the environment is clean except the toilets. Conclusion: Staffs at the facilities are at times ignorant and have negative attitudes at the opening and closing times and facilities have been reported to have long waiting times. The study indicated that the safety and security measures in the urban health facilities are not all practiced according to the National Core Standards checklist/Assessment tool, particularly wet warning signs, disabled toilets, and wearing of nametags. Majority of the participants indicated that, the status of cleanliness was on par at the urban and rural facilities.
- Full Text:
- Authors: Manyela, Nosiphiwo
- Date: 2019
- Subjects: Health facilities
- Language: English
- Type: Thesis , Masters , MPH
- Identifier: http://hdl.handle.net/10353/16747 , vital:40770
- Description: Aim: The purpose of the study was to assess the quality of patient care in the Primary Health Care (PHC) facilities of Mnquma Sub-district with the goal of improving or maintaining quality nursing in the area. Methodology: This study followed a quantitative research approach and it is descriptive in nature. The study involved 561 participants, of whom 406 participants were from urban clinics and 155 were from rural facilities, all these participants were attending clinics at Mnquma Sub-district. A structured questionnaire was used for data collection, since the participants were able to read and write in either English and or IsiXhosa. The data was analyzed with the help of a statistician using Statistical Package for Social Sciences (SPSS) program, software for windows version 21.0 Results: Results showed that participant’s responses to five of the twelve items on patient safety were found to have significant associations with residential setting. Rural participants were less likely to disagree with statements on availability of signage at the entrance of health establishment that indicates times when various services are offered, availability of the security guards to ensure patient safety, warning signs always put in place when the floors are wet, availability of at least one toilet for disabled patients in the health establishment and the fact that health professionals are always wearing name tags. This means that patient safety and security is practiced and implemented at the rural facilities according to the national core standards. The findings of the study confirmed that 13 items out of 19 under care and attitudes were found to have significant associations with residential setting. The items were patients spending less than 2 hours in the health facility; staff treating patients politely, illness being explained clearly to the patients, and ability of the patients to ask questions about their illness. Other items included nursing attitudes during opening and closing times of the health establishment, staff responding well when requested to assist, patients are being treated with empathy, patients are addressed by names and patients are being given all the treatment that they need, nurses explains the treatment side effects, and explains how to deal with side effects. This means that responses to these items depend on whether the patient is from a rural or urban clinic. Rural communities were found to be vi less likely to disagree with statements as compared to urban communities. This means that nurses are compliant with nursing care and attitudes in rural clinics. Findings of the study with respect to facility cleanliness, shows that there is no significant associations between the responses to the items which are: surroundings of the clinic are free of unpleasant smells, grounds of the clinic are clean, safe and clean grounds, bin waste inside and outside storage poses no health risks, and setting were detected. This means that the facilities are and cleanliness status remains the same across the residential settings. However, the younger age group was found be significantly more likely to disagree that toilets are clean and odorless and that grounds are safe and clean compared to the older age group. This suggests that the environment is clean except the toilets. Conclusion: Staffs at the facilities are at times ignorant and have negative attitudes at the opening and closing times and facilities have been reported to have long waiting times. The study indicated that the safety and security measures in the urban health facilities are not all practiced according to the National Core Standards checklist/Assessment tool, particularly wet warning signs, disabled toilets, and wearing of nametags. Majority of the participants indicated that, the status of cleanliness was on par at the urban and rural facilities.
- Full Text:
Assessment of some bacteria species isolated from woodlands of Raymond Mhlaba Local Municipality for high activity laccase production
- Authors: Gogotya, Asemahle
- Date: 2019
- Subjects: Laccase
- Language: English
- Type: Thesis , Masters , MSc (Microbiology)
- Identifier: http://hdl.handle.net/10353/19098 , vital:39879
- Description: The function of enzymes in keeping the earth clean is enormous; being executed in the biodegradation of different natural pollutants and biocatalysis of different responses by substituting the ecologically risky and harmful concoction impetuses offering a situation inviting option, laccases is an example of an enzyme described as it doesn’t produce harmful byproducts. Laccases are employed in several industrial processes that play a key responsibility in transformation of life and making the environment a better place. Bacteria have been described as best producers of laccases with a potential in the industrial scale processes. Laccase was produced from different bacterial isolates identified and named as Bacillus sp. strain GFN1 isolated from soil sample, Bacillus sp. strain GLN and Streptomyces sp. strain LAO both isolated from decaying wood samples in Raymond Mhlaba local municipality with accession numbers MK290988 to MK290990 respectively, as identified by partial sequencing, these were the best producers some of which were positive for Napthol and guaiacol; even upon quantitative screening they were better laccase producers. For quantitative laccase screening, 2,2'-azino-bis(3-ethylbenzothiazoline-6- sulphonic acid) was utilised as the substrate for laccase assays. These laccase producing bacteria were subjected to optimization of growth conditions using submerged fermentation which increased the activity of the produced laccase in great amounts. In optimizing basal medium growth conditions where laccase was harvested after every 72 hours where optimal activity was 16 obtained, studying several factors such as pH which turned out to be pH 5 for all isolates, effect of supplemented carbon and nitrogen sources with the be best being lactose and urea respectively with their effective concentrations using lignin as the main carbon and nitrogen source. Copper sulfate was used as the main inducer and the species preferred guaiacol and ferullic acid and the Fe2+ asthe best supplemented metal ion. The time course was done investigating parameters such as cell growth which was determined by observing the optical density, laccase activity, protein concentration and pH and the presented results suggested that when there was an increase in cell growth, enzyme activity decreased pH had no much effect on the enzyme production as it was almost stable all the time with protein concentration exhibiting no direct effect on enzyme activity also. Characterization of the crude enzyme was done to check the stability of the enzyme produced in various parameters. The enzymes produced by the different strains were thermophilic as they were able to withstand elevated temperatures between 90 and 100 C, with pH stability within an extensive variety of alkaline pH, typical of most bacterial laccases. Various metal ions affected the stability of the enzyme with CuSO4 increasing the stability of two of the bacterial enzyme and appeared to slightly decrease the stability of one enzyme. The studied inhibitors only decreased the stability on the enzyme and couldn’t completely inhibit the enzyme, and the enzymes showed specificity towards varying substrates. The studied bacterial laccases exhibit tremendous characteristics which are of great significance in the industries and will add to the novelty of bacterial laccases and their stability amongst the most studied fungal laccases.
- Full Text:
- Authors: Gogotya, Asemahle
- Date: 2019
- Subjects: Laccase
- Language: English
- Type: Thesis , Masters , MSc (Microbiology)
- Identifier: http://hdl.handle.net/10353/19098 , vital:39879
- Description: The function of enzymes in keeping the earth clean is enormous; being executed in the biodegradation of different natural pollutants and biocatalysis of different responses by substituting the ecologically risky and harmful concoction impetuses offering a situation inviting option, laccases is an example of an enzyme described as it doesn’t produce harmful byproducts. Laccases are employed in several industrial processes that play a key responsibility in transformation of life and making the environment a better place. Bacteria have been described as best producers of laccases with a potential in the industrial scale processes. Laccase was produced from different bacterial isolates identified and named as Bacillus sp. strain GFN1 isolated from soil sample, Bacillus sp. strain GLN and Streptomyces sp. strain LAO both isolated from decaying wood samples in Raymond Mhlaba local municipality with accession numbers MK290988 to MK290990 respectively, as identified by partial sequencing, these were the best producers some of which were positive for Napthol and guaiacol; even upon quantitative screening they were better laccase producers. For quantitative laccase screening, 2,2'-azino-bis(3-ethylbenzothiazoline-6- sulphonic acid) was utilised as the substrate for laccase assays. These laccase producing bacteria were subjected to optimization of growth conditions using submerged fermentation which increased the activity of the produced laccase in great amounts. In optimizing basal medium growth conditions where laccase was harvested after every 72 hours where optimal activity was 16 obtained, studying several factors such as pH which turned out to be pH 5 for all isolates, effect of supplemented carbon and nitrogen sources with the be best being lactose and urea respectively with their effective concentrations using lignin as the main carbon and nitrogen source. Copper sulfate was used as the main inducer and the species preferred guaiacol and ferullic acid and the Fe2+ asthe best supplemented metal ion. The time course was done investigating parameters such as cell growth which was determined by observing the optical density, laccase activity, protein concentration and pH and the presented results suggested that when there was an increase in cell growth, enzyme activity decreased pH had no much effect on the enzyme production as it was almost stable all the time with protein concentration exhibiting no direct effect on enzyme activity also. Characterization of the crude enzyme was done to check the stability of the enzyme produced in various parameters. The enzymes produced by the different strains were thermophilic as they were able to withstand elevated temperatures between 90 and 100 C, with pH stability within an extensive variety of alkaline pH, typical of most bacterial laccases. Various metal ions affected the stability of the enzyme with CuSO4 increasing the stability of two of the bacterial enzyme and appeared to slightly decrease the stability of one enzyme. The studied inhibitors only decreased the stability on the enzyme and couldn’t completely inhibit the enzyme, and the enzymes showed specificity towards varying substrates. The studied bacterial laccases exhibit tremendous characteristics which are of great significance in the industries and will add to the novelty of bacterial laccases and their stability amongst the most studied fungal laccases.
- Full Text:
Assessment of Supply Chain Corruption in the three Metropolitan Municipalities in the Gauteng Province of South Africa
- Authors: Sisi, Molebedi Gordon
- Date: 2019
- Subjects: Government purchasing -- South Africa Political corruption Municipal services
- Language: English
- Type: Thesis , Doctoral , D.Admin
- Identifier: http://hdl.handle.net/10353/14104 , vital:39844
- Description: The aim of the current study was to assess the supply chain corruption in the three metropolitan municipalities in the Gauteng Province of South Africa. Core to the assessment was to establish the causes and ramifications of supply chain corruption as well as the nature and extent of supply chain corruption in the three metropolitan municipalities. In order to meet the objectives of the current the study, a qualitative research approach underpinned by ethics and accountability theories was used. The study employed interviews and observation as a primary source of data collection as well as document review in a form of government documents, legislation and court case decisions as secondary source of data, which were then thematically analysed. The study pointed out that while supply chain corruption certainly negatively affects the day-to-day running of public affairs, it is actually the collapse of ethical behaviour together with the failure to uphold accountability and consequence management that has created fertile ground for supply chain corruption. It further, purported that in order to combat the scourge of supply chain corruption in all its manifestations, there is a need to install a Central Supplier Database application, which has been sufficiently tested to determine its effectiveness as blacklisted companies and their directors have a way of masquerading in another form, to do business with the state. South Africa needs the embodiment of ethical behaviour from its citizenry, along with anti-corruption policy enforcement and consequence management. The Kanyane Ethics Architecture should be launched in municipalities across the board as a comprehensive and all-encompassing mix model to prevent supply chain corruption, in its various manifestations, from taking place.
- Full Text:
- Authors: Sisi, Molebedi Gordon
- Date: 2019
- Subjects: Government purchasing -- South Africa Political corruption Municipal services
- Language: English
- Type: Thesis , Doctoral , D.Admin
- Identifier: http://hdl.handle.net/10353/14104 , vital:39844
- Description: The aim of the current study was to assess the supply chain corruption in the three metropolitan municipalities in the Gauteng Province of South Africa. Core to the assessment was to establish the causes and ramifications of supply chain corruption as well as the nature and extent of supply chain corruption in the three metropolitan municipalities. In order to meet the objectives of the current the study, a qualitative research approach underpinned by ethics and accountability theories was used. The study employed interviews and observation as a primary source of data collection as well as document review in a form of government documents, legislation and court case decisions as secondary source of data, which were then thematically analysed. The study pointed out that while supply chain corruption certainly negatively affects the day-to-day running of public affairs, it is actually the collapse of ethical behaviour together with the failure to uphold accountability and consequence management that has created fertile ground for supply chain corruption. It further, purported that in order to combat the scourge of supply chain corruption in all its manifestations, there is a need to install a Central Supplier Database application, which has been sufficiently tested to determine its effectiveness as blacklisted companies and their directors have a way of masquerading in another form, to do business with the state. South Africa needs the embodiment of ethical behaviour from its citizenry, along with anti-corruption policy enforcement and consequence management. The Kanyane Ethics Architecture should be launched in municipalities across the board as a comprehensive and all-encompassing mix model to prevent supply chain corruption, in its various manifestations, from taking place.
- Full Text:
Assessment Of Supply Chain Corruption In The Three Metropolitan Municipalities, Gauteng Province Of South Africa
- Authors: Sisi, Molebedi Gordon
- Date: 2019
- Subjects: Government purchasing Political corruption| Municipal government
- Language: English
- Type: Thesis , Doctoral , PhD(Public Administration)
- Identifier: http://hdl.handle.net/10353/8343 , vital:32300
- Description: The aim of the current study was to assess the supply chain corruption in the three metropolitan municipalities in the Gauteng Province of South Africa. Core to the assessment was to establish the causes and ramifications of supply chain corruption as well as the nature and extent of supply chain corruption in the three metropolitan municipalities. In order to meet the objectives of the current the study, a qualitative research approach underpinned by ethics and accountability theories was used. The study employed interviews and observation as a primary source of data collection as well as document review in a form of government documents, legislation and court case decisions as secondary source of data, which were then thematically analysed. The study pointed out that while supply chain corruption certainly negatively affects the day-to-day running of public affairs, it is actually the collapse of ethical behaviour together with the failure to uphold accountability and consequence management that has created fertile ground for supply chain corruption. It further, purported that in order to combat the scourge of supply chain corruption in all its manifestations, there is a need to install a Central Supplier Database application, which has been sufficiently tested to determine its effectiveness as blacklisted companies and their directors have a way of masquerading in another form, to do business with the state. South Africa needs the embodiment of ethical behaviour from its citizenry, along with anti-corruption policy enforcement and consequence management. The Kanyane Ethics Architecture should be launched in municipalities across the board as a comprehensive and all-encompassing mix model to prevent supply chain corruption, in its various manifestations, from taking place.
- Full Text:
- Authors: Sisi, Molebedi Gordon
- Date: 2019
- Subjects: Government purchasing Political corruption| Municipal government
- Language: English
- Type: Thesis , Doctoral , PhD(Public Administration)
- Identifier: http://hdl.handle.net/10353/8343 , vital:32300
- Description: The aim of the current study was to assess the supply chain corruption in the three metropolitan municipalities in the Gauteng Province of South Africa. Core to the assessment was to establish the causes and ramifications of supply chain corruption as well as the nature and extent of supply chain corruption in the three metropolitan municipalities. In order to meet the objectives of the current the study, a qualitative research approach underpinned by ethics and accountability theories was used. The study employed interviews and observation as a primary source of data collection as well as document review in a form of government documents, legislation and court case decisions as secondary source of data, which were then thematically analysed. The study pointed out that while supply chain corruption certainly negatively affects the day-to-day running of public affairs, it is actually the collapse of ethical behaviour together with the failure to uphold accountability and consequence management that has created fertile ground for supply chain corruption. It further, purported that in order to combat the scourge of supply chain corruption in all its manifestations, there is a need to install a Central Supplier Database application, which has been sufficiently tested to determine its effectiveness as blacklisted companies and their directors have a way of masquerading in another form, to do business with the state. South Africa needs the embodiment of ethical behaviour from its citizenry, along with anti-corruption policy enforcement and consequence management. The Kanyane Ethics Architecture should be launched in municipalities across the board as a comprehensive and all-encompassing mix model to prevent supply chain corruption, in its various manifestations, from taking place.
- Full Text:
Assessment of the municipal support and intervention programme in selected municipalities of the Eastern Cape
- Authors: Ngwadi, Mzamo
- Date: 2019
- Subjects: Municipal services Public administration|
- Language: English
- Type: Thesis , Doctoral , D.Admin
- Identifier: http://hdl.handle.net/10353/17061 , vital:40844
- Description: The study assesses the outcomes and impact of Municipal Support and Intervention Programme which was implemented in the study sites of Blue Crane Route and Kouga Local Municipalities, which are falling under Sarah Baartman District Municipality, in the Province of Eastern Cape, in the Republic of South Africa. The researcher’s study is an attempt to pursue and record the foot-prints left by Municipal Support and Intervention Programme (MSIP) in these study sites in order to determine the potential extended implementation of the programme in other municipalities with similar challenges of political instability, poor governance, poor audit opinions, unsound financial management systems leading to poor and unsustainable municipal service delivery. The Department of Co-operative Governance and Traditional Affairs (COGTA) conducted a diagnosis on the performance of the then 45municipalities (2007/08 Financial Year) and identified challenges of similar nature but different degrees in fifteen municipalities from the erstwhile Transkei Administration. The municipalities comprised: 2 Metropolitans, 37 Locals and 6 Districts. The diagnostic results compelled COGTA and National Treasury to develop a Municipal Support and Intervention Framework (MSIF) which was piloted in those 15 municipalities then and later adopted as a fully-fledged programme, referred to above as MSIP by the Member of Executive Committee (MEC) for COGTA and Mayors in September, 2014 at Komani (Queenstown). x The concern of the researcher is, in spite of numerous efforts by National and Provincial Departments and Treasuries, it has been observed that some municipalities are still faced with challenges of political instability, poor governance and unsound financial management/financial variability which result to poor municipal service delivery. The latter challenges manifest themselves in protests by the citizenry, poor external audit results due to ineffective political oversight coupled with the lack of scarce and critical professional technical skills. A review of existing literature assists the researcher to identify the key concepts of relevance to the study and assists the investigator to become knowledgeable to the limits, challenges and the language of the type of the research they plan to conduct. The research questions or hypothesis are as follows: • What is the understanding of Municipal Support and Intervention Programme by Blue Crane Route and Kouga Local Municipalities towards service delivery? • Which factors have influenced the effectiveness of the implementation of Municipal Support and Intervention Programme in Blue Crane Route and Kouga Local Municipalities? • What are the recommendations for improved implementation of Municipal Support and Intervention Programme in Blue Crane Route and Kouga Local Municipalities? The aim of this study is to assess the impact of Municipal Support and Intervention Programme (MSIP) Implementation by the Provincial Administration at the municipal level, with special reference to Blue Crane Route and Kouga Municipalities. The objectives of the research are: xi • To explore understanding of the Municipal Support and Intervention Programme in Blue Crane Route and Kouga Local Municipalities. • To explore the factors which influence the effectiveness of the Municipal Support and Intervention Programme in Blue Crane Route and Kouga Local Municipalities • To explore some findings on the study of Municipal Support and Intervention Programme in Blue Crane Route and Kouga Local Municipalities. • To explore recommendations towards improving the implementation of the Municipal Support and Intervention Programme in Blue Crane Route and Kouga Local Municipalities. Qualitative and Quantitative Methodologies were utilised for data gathering in the two study sites. The study concluded that when the above-mentioned challenges are existing in a municipality, they will manifest themselves through the demonstrations by citizenry which sometimes lead to damage of infrastructure. It is therefore, recommended that the three spheres of government should go back to the drawing board when deploying councillors in municipality by considering basic relevant educational qualifications because the Local Government Sector is highly legislated and complicated. The learning curve is sometimes a bit longer during a five-year elections term. This should be the same considerations when technocrats are being appointed at the level of Section 54 and 56, in terms of Municipal Systems Act, No. 32 of 2000, especially in the area of scarce and critical professional skills. In the case of educational, training and development gaps, the National Department of Co-operative Governance, National Treasury and the South African Local Government Association (SALGA), could enhance upskilling supporting politicians and municipal senior managers with accredited and refresher courses
- Full Text:
- Authors: Ngwadi, Mzamo
- Date: 2019
- Subjects: Municipal services Public administration|
- Language: English
- Type: Thesis , Doctoral , D.Admin
- Identifier: http://hdl.handle.net/10353/17061 , vital:40844
- Description: The study assesses the outcomes and impact of Municipal Support and Intervention Programme which was implemented in the study sites of Blue Crane Route and Kouga Local Municipalities, which are falling under Sarah Baartman District Municipality, in the Province of Eastern Cape, in the Republic of South Africa. The researcher’s study is an attempt to pursue and record the foot-prints left by Municipal Support and Intervention Programme (MSIP) in these study sites in order to determine the potential extended implementation of the programme in other municipalities with similar challenges of political instability, poor governance, poor audit opinions, unsound financial management systems leading to poor and unsustainable municipal service delivery. The Department of Co-operative Governance and Traditional Affairs (COGTA) conducted a diagnosis on the performance of the then 45municipalities (2007/08 Financial Year) and identified challenges of similar nature but different degrees in fifteen municipalities from the erstwhile Transkei Administration. The municipalities comprised: 2 Metropolitans, 37 Locals and 6 Districts. The diagnostic results compelled COGTA and National Treasury to develop a Municipal Support and Intervention Framework (MSIF) which was piloted in those 15 municipalities then and later adopted as a fully-fledged programme, referred to above as MSIP by the Member of Executive Committee (MEC) for COGTA and Mayors in September, 2014 at Komani (Queenstown). x The concern of the researcher is, in spite of numerous efforts by National and Provincial Departments and Treasuries, it has been observed that some municipalities are still faced with challenges of political instability, poor governance and unsound financial management/financial variability which result to poor municipal service delivery. The latter challenges manifest themselves in protests by the citizenry, poor external audit results due to ineffective political oversight coupled with the lack of scarce and critical professional technical skills. A review of existing literature assists the researcher to identify the key concepts of relevance to the study and assists the investigator to become knowledgeable to the limits, challenges and the language of the type of the research they plan to conduct. The research questions or hypothesis are as follows: • What is the understanding of Municipal Support and Intervention Programme by Blue Crane Route and Kouga Local Municipalities towards service delivery? • Which factors have influenced the effectiveness of the implementation of Municipal Support and Intervention Programme in Blue Crane Route and Kouga Local Municipalities? • What are the recommendations for improved implementation of Municipal Support and Intervention Programme in Blue Crane Route and Kouga Local Municipalities? The aim of this study is to assess the impact of Municipal Support and Intervention Programme (MSIP) Implementation by the Provincial Administration at the municipal level, with special reference to Blue Crane Route and Kouga Municipalities. The objectives of the research are: xi • To explore understanding of the Municipal Support and Intervention Programme in Blue Crane Route and Kouga Local Municipalities. • To explore the factors which influence the effectiveness of the Municipal Support and Intervention Programme in Blue Crane Route and Kouga Local Municipalities • To explore some findings on the study of Municipal Support and Intervention Programme in Blue Crane Route and Kouga Local Municipalities. • To explore recommendations towards improving the implementation of the Municipal Support and Intervention Programme in Blue Crane Route and Kouga Local Municipalities. Qualitative and Quantitative Methodologies were utilised for data gathering in the two study sites. The study concluded that when the above-mentioned challenges are existing in a municipality, they will manifest themselves through the demonstrations by citizenry which sometimes lead to damage of infrastructure. It is therefore, recommended that the three spheres of government should go back to the drawing board when deploying councillors in municipality by considering basic relevant educational qualifications because the Local Government Sector is highly legislated and complicated. The learning curve is sometimes a bit longer during a five-year elections term. This should be the same considerations when technocrats are being appointed at the level of Section 54 and 56, in terms of Municipal Systems Act, No. 32 of 2000, especially in the area of scarce and critical professional skills. In the case of educational, training and development gaps, the National Department of Co-operative Governance, National Treasury and the South African Local Government Association (SALGA), could enhance upskilling supporting politicians and municipal senior managers with accredited and refresher courses
- Full Text:
Assessment of the role of the board of directors in the implementation of corporate governance in the state owned entities: a case in the Eastern Cape Province
- Authors: Makhala, Nombuyiselo
- Date: 2019
- Subjects: Corporate governance Directors of corporations
- Language: English
- Type: Thesis , Doctoral , D.Admin
- Identifier: http://hdl.handle.net/10353/13642 , vital:39687
- Description: Corporate governance is a well-recognized governing method, which ensures that the organization achieves its goals. This concept has received too much attention due to the number of reported corporate scandals in both public and private entities. Due to these highly publicized governance failures, the role of Board of Directors in the implementation of corporate governance has been questioned. Their role has been the topic of interest. The main aim of this study was to assess the role of the Board of Directors in the implementation of Corporate Governance in the State Owned Entities in the Eastern Cape Province. It also aimed to determine the strategies and mechanisms that can be used to enhance the role played by the Board of directors in the successful implementation of corporate governance in the public entities. This research was qualitative in nature and as part of this approach data was gathered through questionnaires and interviews. For this study, small scale and manageable sample was used. The sample was selected with the view that all the participants selected were experienced and directly involved in the policy and decision-making as well as in implementation of corporate governance in the state-owned entities. The secondary data was collected through the audit reports, performance, and annual reports of the different public entities. The findings of this study revealed that some Board of Directors are unable to play the effective role in the implementation of corporate governance due to lack of experience and skills required. The research has also revealed that the Boards are not effective in their role due to lax in enforcing accountability by the shareholder. The findings on poor performance management of the Boards is because of no standardized performance management system for the Eastern Cape Province public entities in place. Political deployment of board members had an impact in the independency and objectivity of the boards. The study recommends the appointment of skilled and qualifying board members, training and induction of newly appointed be conducted. Development of accountability framework for all the boards of the public entities in the Eastern Cape. For the Boards to successfully play an effective role in the implementation of corporate governance, adequate budget should be allocated to the public entities. Lastly, the development of the standardized performance management system and framework with prescribed key responsibility areas for all the Boards of the state owned entities will assist the Boards in knowing what is expected of them.
- Full Text:
- Authors: Makhala, Nombuyiselo
- Date: 2019
- Subjects: Corporate governance Directors of corporations
- Language: English
- Type: Thesis , Doctoral , D.Admin
- Identifier: http://hdl.handle.net/10353/13642 , vital:39687
- Description: Corporate governance is a well-recognized governing method, which ensures that the organization achieves its goals. This concept has received too much attention due to the number of reported corporate scandals in both public and private entities. Due to these highly publicized governance failures, the role of Board of Directors in the implementation of corporate governance has been questioned. Their role has been the topic of interest. The main aim of this study was to assess the role of the Board of Directors in the implementation of Corporate Governance in the State Owned Entities in the Eastern Cape Province. It also aimed to determine the strategies and mechanisms that can be used to enhance the role played by the Board of directors in the successful implementation of corporate governance in the public entities. This research was qualitative in nature and as part of this approach data was gathered through questionnaires and interviews. For this study, small scale and manageable sample was used. The sample was selected with the view that all the participants selected were experienced and directly involved in the policy and decision-making as well as in implementation of corporate governance in the state-owned entities. The secondary data was collected through the audit reports, performance, and annual reports of the different public entities. The findings of this study revealed that some Board of Directors are unable to play the effective role in the implementation of corporate governance due to lack of experience and skills required. The research has also revealed that the Boards are not effective in their role due to lax in enforcing accountability by the shareholder. The findings on poor performance management of the Boards is because of no standardized performance management system for the Eastern Cape Province public entities in place. Political deployment of board members had an impact in the independency and objectivity of the boards. The study recommends the appointment of skilled and qualifying board members, training and induction of newly appointed be conducted. Development of accountability framework for all the boards of the public entities in the Eastern Cape. For the Boards to successfully play an effective role in the implementation of corporate governance, adequate budget should be allocated to the public entities. Lastly, the development of the standardized performance management system and framework with prescribed key responsibility areas for all the Boards of the state owned entities will assist the Boards in knowing what is expected of them.
- Full Text:
Benefits and limitation of informal trading in promoting sustainable local economic development in the Intsika Yethu Municipality
- Authors: Makubalo, Zukiswa
- Date: 2019
- Subjects: Municipal government -- South Africa -- Eastern Cape Sustainable development -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MPA
- Identifier: http://hdl.handle.net/10353/12425 , vital:39262
- Description: The informal sector refers to all economic activities by workers and economic units that are not covered or insufficiently covered by formal arrangements which can be carried out across all the sectors of the economy both in public and private spaces. The study was conducted to determine the benefits and limitation of informal trading in promoting sustainable local economic development in the Intsika Yethu Municipality. South African municipalities for various reasons fail in providing local economic development friendly environment and more developmental and inclusive informal economy policies and by-laws. The objective of the study was to assess the benefits and challenges which might hinder the maximisation of benefits in the sector. The literature was reviewed with the aim to integrate published academic data on the benefits and limitations of informal trading in promoting sustainable local economic development. Data for the study was sourced from the literature and questionnaires administered to respondents who were selected by means of a purposive sampling technique. The respondents from which data was collected were 18 and a qualitative research approach was used as a main research methodology. The responses obtained were subjected to content analyses. The main findings of the study include; informal traders are uncoordinated, informal traders are faced with challenges which hinder their success, majority of respondents see benefits from informal trading, the informal sector of responds to triple social ills and all respondents started up businesses due to the inability of the formal sector to create job opportunities that are permanent and enough for everyone. In response to the findings about the main recommendation made is the improvement of communication between government and informal trader for the sustainability of municipal programs.
- Full Text:
- Authors: Makubalo, Zukiswa
- Date: 2019
- Subjects: Municipal government -- South Africa -- Eastern Cape Sustainable development -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MPA
- Identifier: http://hdl.handle.net/10353/12425 , vital:39262
- Description: The informal sector refers to all economic activities by workers and economic units that are not covered or insufficiently covered by formal arrangements which can be carried out across all the sectors of the economy both in public and private spaces. The study was conducted to determine the benefits and limitation of informal trading in promoting sustainable local economic development in the Intsika Yethu Municipality. South African municipalities for various reasons fail in providing local economic development friendly environment and more developmental and inclusive informal economy policies and by-laws. The objective of the study was to assess the benefits and challenges which might hinder the maximisation of benefits in the sector. The literature was reviewed with the aim to integrate published academic data on the benefits and limitations of informal trading in promoting sustainable local economic development. Data for the study was sourced from the literature and questionnaires administered to respondents who were selected by means of a purposive sampling technique. The respondents from which data was collected were 18 and a qualitative research approach was used as a main research methodology. The responses obtained were subjected to content analyses. The main findings of the study include; informal traders are uncoordinated, informal traders are faced with challenges which hinder their success, majority of respondents see benefits from informal trading, the informal sector of responds to triple social ills and all respondents started up businesses due to the inability of the formal sector to create job opportunities that are permanent and enough for everyone. In response to the findings about the main recommendation made is the improvement of communication between government and informal trader for the sustainability of municipal programs.
- Full Text:
Bioavailability of nutrients in a diet enriched with moringa oleifera lam. Leaves using wistar rats
- Authors: Mhlomi, Yanga Nonelela
- Date: 2019
- Subjects: Moringa Medicinal plants
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/14594 , vital:40018
- Description: This study investigated the nutritional composition and bioavailability of nutrients in Moringa oleifera leaf meal from South African ecotype. Nutritional evaluation (proximate, mineral, vitamins and antinutrients, amino acids and fatty acids analyses) was performed using AOAC, ICP-OES and GC+MS techniques respectively. Bioavailability of nutrients involved formulation of diets deficient in minerals and vitamins; and proteins supplemented with Moringa oleifera leaf meal (MOLM) at 0, 3, 5 and 10% respectively. Growth performance, hematological, biochemical and histopathological evaluations were used to assess the effect of MOLM supplementation. Proximate and mineral evaluation revealed that the leaves were rich in protein (28.72%) and carbohydrate (28.84%), calcium content was (1603.33 mg/100g), potassium (1690 mg/100g), zinc (13.03 mg/100g) and iron (21.13 mg/100g). Vitamin E content was the highest (89.43 mg/100g) followed by vitamic C (24.9 mg/100g) and vitamin A (12.98 mg/100g). Fatty acids analysis detected were fifteen of which polyunsaturated fatty acid linoleic acid (56.36) and alpha-linolenic acid (687.58) were highest and behenic acid (22:0) characteristics of Moringa (4.11%) were detected. Seven of the detected fatty acids were saturated fatty acids, though relatively low, with stearic acid having the highest value. On the other hand fifteen of the amino acids, 7 were essential (valine, leucine, isoleucine, methionine, threonine and phenylalanine) were detected; while alanine, proline, serine, aspartic acid, cysteine, glutamic acid, asparagine and tyrosine were non- essential amino acid. Moringa oleifera had low quantities of antinutrients in relation to minerals, thus nutrients will easily ne available. Amino and fatty acids profile of nutrient deficient diets supplemented with MOLM revealed that six essential amino acids were detected in the diets. However, the essential amino acid (EAA) in MOLM with the highest value was threonine (500.47±23.03mg/L), while the lowest was methionine (75.65±5.31mg/L). Leucine was highest in PD10 (234.32±27.98 mg/l) 15 and lowest in PD3. Predominant amino acid in VMD diet was glutamic acid (966.76±208.92 mg/l), while methionine was lowest (197.14±35.90). Caproic acid was the most prominent unsaturated fatty acid in the diets and was highest in the PD diet. Palmitoleic acid was highest in the control diet (8.19±0.56 mg/L) and lowest in PD3 but an increase was observed as the levels of MOLM increased in the diets. Linoleic acid values obtained were significantly higher in PD diet, lower in control diet, compared to other diets. Linolenic acid (C18-3n6) was higher in PD10 (63.48±3.84 mg/L) compared to the control diet (6.05±0.14 mg/L). The highest unsaturated fatty acid in all the feed samples was linoleic acids ranging from 54.48±5.61 to 296.26±7.20 mg/L. the highest omega-3 (n-3) fatty acid content (63.48±3.38 mg/L) was in PD10 and was almost 10 times higher than the value recorded for control. Rats treated on vitamin mineral deficient (VMD) diet showed no significant changes in growth performances and blood parameters except in certain indices. Rats on VMD diet had lowest haemoglobin levels, whereas haemoglobin increased with MOLM levels. No significant differences in haematocrit, MCH, MCHC, platelets, RDW and MPV were observed in all treatments and control. However, VMD rats showed decrease in haemoglobin, increase in WBC and platelets. Electrolytes and glucose for all treatments and control were not significantly different, but, control and VMD10 diets rats showed significantly higher values for creatinine compared to MOLM diets; total protein and albumin increased with MOLM and were higher than in VMD and control rats. Serum activities of liver, cardiac, adrenal marker enzymes were significantly lower in MOLM rats compared to VMD and control. Mild congestion in VMD and VMD3 rat’s liver and diffused congestion in the kidney of VMD diet fed rats was observed. Protein deficient diets supplemented with MOLM had a negative impact on feed intake and growth parameters. Rats fed PD and PD5 consumed significantly less diet than those fed PD10, PD3 diets respectively. White blood cells decreased with increasing levels of MOLM 16 supplementation. Differential white cell count (Neutrophils, lymphocytes, monocytes, eosinophils and basophils) followed the same trend though not statistically significant (P>0.05). Furthermore, histopathological evaluation of organs revealed that no abnormalities were in heart and liver and kidney of rats fed on the control diet, while mild congestion in the liver and myocardium (heart) were observed in rats fed with protein deficient diet. No visible abnormalities were observed in the heart, kidney and liver of animals on the PD 10 diet, but myocardial fibres showing intense eosinophilia was detected in heart muscles of rats fed PD and PD3 diets. In this study, MOLM had a negative impact on feed intake and growth performance of animals and haematology. Blood biochemistry and histology suggest that MOLM has the potential of defending the body against infection with hematinic and blood enhancing qualities. Protein deficiency led to a significant elevation in the serum lipid profile of rats especially cholesterol even with increase in MOLM. PD10 had the highest triglycerides content when compared to the control but was lower (p>0.05) to that of PD. There was an increase in crude fat, ADF, NDF and protein in faecal samples of rats supplemented with MOLM diets while a decrease in moisture and ash was observed compared to the control.Vitamin and mineral deficiency resulted in significant decrease in serum cholesterol, triglycerides and HDL-C when compared to the control. There was a slight increase in excretion for all elements with increasing MOLM. Total removal of minerals and vitamins from the VMD experimental diets seemed not to have any visible negative impact on performance of the rats probably because of residual micronutrients present in other ingredients used in the feed formulation. MOLM could not replace protein in protein deficient diets.
- Full Text:
- Authors: Mhlomi, Yanga Nonelela
- Date: 2019
- Subjects: Moringa Medicinal plants
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/14594 , vital:40018
- Description: This study investigated the nutritional composition and bioavailability of nutrients in Moringa oleifera leaf meal from South African ecotype. Nutritional evaluation (proximate, mineral, vitamins and antinutrients, amino acids and fatty acids analyses) was performed using AOAC, ICP-OES and GC+MS techniques respectively. Bioavailability of nutrients involved formulation of diets deficient in minerals and vitamins; and proteins supplemented with Moringa oleifera leaf meal (MOLM) at 0, 3, 5 and 10% respectively. Growth performance, hematological, biochemical and histopathological evaluations were used to assess the effect of MOLM supplementation. Proximate and mineral evaluation revealed that the leaves were rich in protein (28.72%) and carbohydrate (28.84%), calcium content was (1603.33 mg/100g), potassium (1690 mg/100g), zinc (13.03 mg/100g) and iron (21.13 mg/100g). Vitamin E content was the highest (89.43 mg/100g) followed by vitamic C (24.9 mg/100g) and vitamin A (12.98 mg/100g). Fatty acids analysis detected were fifteen of which polyunsaturated fatty acid linoleic acid (56.36) and alpha-linolenic acid (687.58) were highest and behenic acid (22:0) characteristics of Moringa (4.11%) were detected. Seven of the detected fatty acids were saturated fatty acids, though relatively low, with stearic acid having the highest value. On the other hand fifteen of the amino acids, 7 were essential (valine, leucine, isoleucine, methionine, threonine and phenylalanine) were detected; while alanine, proline, serine, aspartic acid, cysteine, glutamic acid, asparagine and tyrosine were non- essential amino acid. Moringa oleifera had low quantities of antinutrients in relation to minerals, thus nutrients will easily ne available. Amino and fatty acids profile of nutrient deficient diets supplemented with MOLM revealed that six essential amino acids were detected in the diets. However, the essential amino acid (EAA) in MOLM with the highest value was threonine (500.47±23.03mg/L), while the lowest was methionine (75.65±5.31mg/L). Leucine was highest in PD10 (234.32±27.98 mg/l) 15 and lowest in PD3. Predominant amino acid in VMD diet was glutamic acid (966.76±208.92 mg/l), while methionine was lowest (197.14±35.90). Caproic acid was the most prominent unsaturated fatty acid in the diets and was highest in the PD diet. Palmitoleic acid was highest in the control diet (8.19±0.56 mg/L) and lowest in PD3 but an increase was observed as the levels of MOLM increased in the diets. Linoleic acid values obtained were significantly higher in PD diet, lower in control diet, compared to other diets. Linolenic acid (C18-3n6) was higher in PD10 (63.48±3.84 mg/L) compared to the control diet (6.05±0.14 mg/L). The highest unsaturated fatty acid in all the feed samples was linoleic acids ranging from 54.48±5.61 to 296.26±7.20 mg/L. the highest omega-3 (n-3) fatty acid content (63.48±3.38 mg/L) was in PD10 and was almost 10 times higher than the value recorded for control. Rats treated on vitamin mineral deficient (VMD) diet showed no significant changes in growth performances and blood parameters except in certain indices. Rats on VMD diet had lowest haemoglobin levels, whereas haemoglobin increased with MOLM levels. No significant differences in haematocrit, MCH, MCHC, platelets, RDW and MPV were observed in all treatments and control. However, VMD rats showed decrease in haemoglobin, increase in WBC and platelets. Electrolytes and glucose for all treatments and control were not significantly different, but, control and VMD10 diets rats showed significantly higher values for creatinine compared to MOLM diets; total protein and albumin increased with MOLM and were higher than in VMD and control rats. Serum activities of liver, cardiac, adrenal marker enzymes were significantly lower in MOLM rats compared to VMD and control. Mild congestion in VMD and VMD3 rat’s liver and diffused congestion in the kidney of VMD diet fed rats was observed. Protein deficient diets supplemented with MOLM had a negative impact on feed intake and growth parameters. Rats fed PD and PD5 consumed significantly less diet than those fed PD10, PD3 diets respectively. White blood cells decreased with increasing levels of MOLM 16 supplementation. Differential white cell count (Neutrophils, lymphocytes, monocytes, eosinophils and basophils) followed the same trend though not statistically significant (P>0.05). Furthermore, histopathological evaluation of organs revealed that no abnormalities were in heart and liver and kidney of rats fed on the control diet, while mild congestion in the liver and myocardium (heart) were observed in rats fed with protein deficient diet. No visible abnormalities were observed in the heart, kidney and liver of animals on the PD 10 diet, but myocardial fibres showing intense eosinophilia was detected in heart muscles of rats fed PD and PD3 diets. In this study, MOLM had a negative impact on feed intake and growth performance of animals and haematology. Blood biochemistry and histology suggest that MOLM has the potential of defending the body against infection with hematinic and blood enhancing qualities. Protein deficiency led to a significant elevation in the serum lipid profile of rats especially cholesterol even with increase in MOLM. PD10 had the highest triglycerides content when compared to the control but was lower (p>0.05) to that of PD. There was an increase in crude fat, ADF, NDF and protein in faecal samples of rats supplemented with MOLM diets while a decrease in moisture and ash was observed compared to the control.Vitamin and mineral deficiency resulted in significant decrease in serum cholesterol, triglycerides and HDL-C when compared to the control. There was a slight increase in excretion for all elements with increasing MOLM. Total removal of minerals and vitamins from the VMD experimental diets seemed not to have any visible negative impact on performance of the rats probably because of residual micronutrients present in other ingredients used in the feed formulation. MOLM could not replace protein in protein deficient diets.
- Full Text:
Bioinformatic analysis, isolation and kinetic characterisation of red algae (Gelidium capense) dehydrogenases
- Authors: Gogela, Yanga
- Date: 2019
- Subjects: Bioinformatics Chondrus crispus
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/19164 , vital:39878
- Description: Lactate and alcohol dehydrogenases have attracted much attention in various industries and scientific research for their ability to produce chirally pure compounds and be assayed for activity using more straightforward and reproducible assay methods. These enzymes have been previously isolated and purified from various plants, animals and microorganisms. So far, the molecular and biochemical properties of enzymes from these dehydrogenase families in red algae are mostly unknown. Red macroalgae have been used for centuries for the treatment of various diseases and as a source of ingredients in the food industry. The aim of this study was to identify genes in the sequenced red algae genomes that encode dehydrogenases, to use bioinformatic tools to confirm that the proteins encoded are dehydrogenases and to isolate and kinetically purify alcohol or lactate dehydrogenase from red algae species found along the coastline of the Eastern Cape Province. A combination of bioinformatics tools, molecular and biochemical techniques were used to identify, purify, and characterise ADH and LDH enzymes. Bioinformatics analysis revealed two alcohol dehydrogenase genes and two hypothetical genes encoding functional domains similar to D-lactate dehydrogenases from other species. The ADH and LDH-like genes shared low sequence identity at the protein level with medium-chain dehydrogenases/reductases (MDRs) and 2-hydroxy acid dehydrogenases, respectively. These two dehydrogenase genes showed a highly conserved NAD-binding motif (Rossmann-fold) similar to many other NAD-dependent dehydrogenases. The ADH and LDH proteins contained no signal peptides and may be located in the cytoplasm. The phylogenetic tree analysis showed that the two ADH genes belonged to cinnamyl and class III alcohol dehydrogenases, whereas the LDHlike genes were grouped with D-lactate dehydrogenases from other organisms. The ADH and LDH gene family showed cis-acting regulatory elements that are mostly involved in stress response and hormonal response. Structural analysis showed that the dehydrogenases 3D structure predicted models comprise of two domains, namely the substrate binding and the coenzyme binding domains that are rich in beta-strands secondary structure elements. The LDH from red algae was purified approximately 4-fold with a specific activity of 0.044 U/mg. The purified LDH enzyme had a molecular weight of approximately 37kDa. The LDH was active across a broad pH range from 5-9 with a pH optimum observed at 7.5. The LDH ii enzyme in red algae exhibits a temperature optimum of 40 ⁰C and heat stability up to 40 ⁰C. Above 50 °C the LDH activity rapidly decreased showing that the LDH in red algae is not thermostable. The LDH enzyme showed a Km value of 0.8 mM and Vmax of 0.0067 mM.min-1 when using sodium pyruvate as a substrate.
- Full Text:
- Authors: Gogela, Yanga
- Date: 2019
- Subjects: Bioinformatics Chondrus crispus
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/19164 , vital:39878
- Description: Lactate and alcohol dehydrogenases have attracted much attention in various industries and scientific research for their ability to produce chirally pure compounds and be assayed for activity using more straightforward and reproducible assay methods. These enzymes have been previously isolated and purified from various plants, animals and microorganisms. So far, the molecular and biochemical properties of enzymes from these dehydrogenase families in red algae are mostly unknown. Red macroalgae have been used for centuries for the treatment of various diseases and as a source of ingredients in the food industry. The aim of this study was to identify genes in the sequenced red algae genomes that encode dehydrogenases, to use bioinformatic tools to confirm that the proteins encoded are dehydrogenases and to isolate and kinetically purify alcohol or lactate dehydrogenase from red algae species found along the coastline of the Eastern Cape Province. A combination of bioinformatics tools, molecular and biochemical techniques were used to identify, purify, and characterise ADH and LDH enzymes. Bioinformatics analysis revealed two alcohol dehydrogenase genes and two hypothetical genes encoding functional domains similar to D-lactate dehydrogenases from other species. The ADH and LDH-like genes shared low sequence identity at the protein level with medium-chain dehydrogenases/reductases (MDRs) and 2-hydroxy acid dehydrogenases, respectively. These two dehydrogenase genes showed a highly conserved NAD-binding motif (Rossmann-fold) similar to many other NAD-dependent dehydrogenases. The ADH and LDH proteins contained no signal peptides and may be located in the cytoplasm. The phylogenetic tree analysis showed that the two ADH genes belonged to cinnamyl and class III alcohol dehydrogenases, whereas the LDHlike genes were grouped with D-lactate dehydrogenases from other organisms. The ADH and LDH gene family showed cis-acting regulatory elements that are mostly involved in stress response and hormonal response. Structural analysis showed that the dehydrogenases 3D structure predicted models comprise of two domains, namely the substrate binding and the coenzyme binding domains that are rich in beta-strands secondary structure elements. The LDH from red algae was purified approximately 4-fold with a specific activity of 0.044 U/mg. The purified LDH enzyme had a molecular weight of approximately 37kDa. The LDH was active across a broad pH range from 5-9 with a pH optimum observed at 7.5. The LDH ii enzyme in red algae exhibits a temperature optimum of 40 ⁰C and heat stability up to 40 ⁰C. Above 50 °C the LDH activity rapidly decreased showing that the LDH in red algae is not thermostable. The LDH enzyme showed a Km value of 0.8 mM and Vmax of 0.0067 mM.min-1 when using sodium pyruvate as a substrate.
- Full Text:
Caregivers’ and parents’ views about preschool education as a school readiness programme in the Queenstown Education District
- Mtyuda, Pamela Nomonde Pettina
- Authors: Mtyuda, Pamela Nomonde Pettina
- Date: 2019
- Subjects: Education, Preschool Readiness for school
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10353/16166 , vital:40674
- Description: The aim of this study was to uncover preschool caregivers’ and parents’ views about preschool education as a preschool readiness programme in the Queenstown education district. The research study addressed the following specific objectives, to determine: the caregivers’ and parents’ understanding of school readiness nature of preschool education; the extent to which the caregivers’ practices can make children ready for formal schooling; the parents’ assessment of the preschool centres as places that make children ready for formal schooling; caregivers’ and parents’ views on the challenges facing preschool centres against making children ready for formal schooling; and lastly, the possible solutions to the challenges facing preschool centres. In the present study, both qualitative and quantitative data were collected. Quantitative data were gathered through questionnaires from 40 preschool caregivers at preschools and 40 parents of the children in those preschools that were chosen by the caregivers on the basis of parents who are highly committed in the education of their children. The qualitative data were gathered through focus group discussions with 2 focus groups located in the Queenstown Education district. Twelve participants were interviewed, and these comprised 6 caregivers that formed the focus group discussion 1 and 6 parents that formed the focus group discussion 2, using the interview schedules as guides. Purposive sampling to select the participants was used. Triangulation design was used to gather both quantitative and qualitative data at the same time and to integrate the two forms of data in order to have a better understanding of the research objectives. The research findings indicated that the most frequently reported issues by the participants were the problems with some caregivers who had limited knowledge to make children ready for formal schooling. Some of the parents and caregivers do not understand the importance of the domains of development in making children fully ready for formal schooling. There is still lack of parental involvement in those centres; no effective communication between parents and staff; caregivers are not aware of the policies that govern the preschools; poverty and unemployment are factors that inhibit the right of young children to education; no proper resources in preschools and the unregistered preschools also still need to be helped by coordinated efforts from communities and the government bodies. It was concluded that the quality of early childhood development(ECD) programmes in Queenstown preschools was still compromised. The study recommended extensive investment in infrastructure and learning and teaching support materials is necessary; there should be sufficient staffing with ECD expertise; programmes should be designed in order to educate and encourage parents to actively involve themselves with preschool activities.
- Full Text:
- Authors: Mtyuda, Pamela Nomonde Pettina
- Date: 2019
- Subjects: Education, Preschool Readiness for school
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10353/16166 , vital:40674
- Description: The aim of this study was to uncover preschool caregivers’ and parents’ views about preschool education as a preschool readiness programme in the Queenstown education district. The research study addressed the following specific objectives, to determine: the caregivers’ and parents’ understanding of school readiness nature of preschool education; the extent to which the caregivers’ practices can make children ready for formal schooling; the parents’ assessment of the preschool centres as places that make children ready for formal schooling; caregivers’ and parents’ views on the challenges facing preschool centres against making children ready for formal schooling; and lastly, the possible solutions to the challenges facing preschool centres. In the present study, both qualitative and quantitative data were collected. Quantitative data were gathered through questionnaires from 40 preschool caregivers at preschools and 40 parents of the children in those preschools that were chosen by the caregivers on the basis of parents who are highly committed in the education of their children. The qualitative data were gathered through focus group discussions with 2 focus groups located in the Queenstown Education district. Twelve participants were interviewed, and these comprised 6 caregivers that formed the focus group discussion 1 and 6 parents that formed the focus group discussion 2, using the interview schedules as guides. Purposive sampling to select the participants was used. Triangulation design was used to gather both quantitative and qualitative data at the same time and to integrate the two forms of data in order to have a better understanding of the research objectives. The research findings indicated that the most frequently reported issues by the participants were the problems with some caregivers who had limited knowledge to make children ready for formal schooling. Some of the parents and caregivers do not understand the importance of the domains of development in making children fully ready for formal schooling. There is still lack of parental involvement in those centres; no effective communication between parents and staff; caregivers are not aware of the policies that govern the preschools; poverty and unemployment are factors that inhibit the right of young children to education; no proper resources in preschools and the unregistered preschools also still need to be helped by coordinated efforts from communities and the government bodies. It was concluded that the quality of early childhood development(ECD) programmes in Queenstown preschools was still compromised. The study recommended extensive investment in infrastructure and learning and teaching support materials is necessary; there should be sufficient staffing with ECD expertise; programmes should be designed in order to educate and encourage parents to actively involve themselves with preschool activities.
- Full Text:
Challenges faced by grandparents in caring for their grandchildren in Mdlankomo location, Libode, Eastern Cape
- Authors: Soganga, Aseza
- Date: 2019
- Subjects: Grandparents as parents Grandchildren -- Care
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10353/16188 , vital:40692
- Description: The study aimed to investigate the challenges that older grandparents face in caring for their grandchildren in Mdlankomo Location, Libode, Eastern Cape. The study intended to achieve three (3) objectives: (i) to establish factors that make caregiving of children by their grandparents challenging, (ii) to establish resources that grandparents need to care for their grandchildren and (iii) to establish the availability of resources required by the grandparents to care for their grandchildren and its possible gaps. The study utilized qualitative research approach, and date collection methods used were in-depth interviews, focus group discussion and key informant method. The number of participants was 34 in total consisting of 23 grandparents and 11 key informants. The findings of the study showed that death of children’s own parents; cultural expectations, youth unemployment, abdication of parental responsibilities, youth unemployment, and carelessness of biological parents were the major reasons for grandparents to care for their grandchildren. The study findings also revealed that grandparents in this caregiving experience both benefits and barriers. The findings further revealed that there are gaps in welfare services, medical services and education. Therefore, the researcher recommended that, those in power should be interested, eager and inquisitive enough about certain aspects that need attention in caregiving of children by their grandparents.
- Full Text:
- Authors: Soganga, Aseza
- Date: 2019
- Subjects: Grandparents as parents Grandchildren -- Care
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10353/16188 , vital:40692
- Description: The study aimed to investigate the challenges that older grandparents face in caring for their grandchildren in Mdlankomo Location, Libode, Eastern Cape. The study intended to achieve three (3) objectives: (i) to establish factors that make caregiving of children by their grandparents challenging, (ii) to establish resources that grandparents need to care for their grandchildren and (iii) to establish the availability of resources required by the grandparents to care for their grandchildren and its possible gaps. The study utilized qualitative research approach, and date collection methods used were in-depth interviews, focus group discussion and key informant method. The number of participants was 34 in total consisting of 23 grandparents and 11 key informants. The findings of the study showed that death of children’s own parents; cultural expectations, youth unemployment, abdication of parental responsibilities, youth unemployment, and carelessness of biological parents were the major reasons for grandparents to care for their grandchildren. The study findings also revealed that grandparents in this caregiving experience both benefits and barriers. The findings further revealed that there are gaps in welfare services, medical services and education. Therefore, the researcher recommended that, those in power should be interested, eager and inquisitive enough about certain aspects that need attention in caregiving of children by their grandparents.
- Full Text:
Challenges in the implementation of a community police forum constitution: a case study of the beacon bay community police forum in the Eastern Cape
- Authors: Twani, Sydney Vuyisile
- Date: 2019
- Subjects: Community policing -- South Africa Crime prevention Police-community relations Police -- South Africa
- Language: English
- Type: Thesis , Masters , MPA
- Identifier: http://hdl.handle.net/10353/11031 , vital:36687
- Description: The purpose of this study was to investigate the extent to which the Beacon Bay Community Police Forum in the East London area has implemented the Community Police Forum (CPF) uniform constitution. The main research question of this study was: To what extent has the Beacon Bay Community Police Forum in the East London area implemented the uniform Community Police Forum (CPF) constitution? The theoretical framework that guided this study was the broken windows theory. The literature review looked into the following: existing knowledge based on the assessment of the implementation of the uniform CPF constitution by the Beacon Bay Community Police Forum, the regulatory framework and an overview of the role and functions of the community police forum (CPF) in South Africa, lack of awareness and representativeness as challenges to community police forums effectiveness, inadequate resources in the implementation of an effective community policing forum, lack of trust between the police and the community and the issue of corruption as challenges in the implementation of CPF. The qualitative research method was used in this study. The case study design was also used. The researcher used the purposive sampling technique to select six community members and four community executives of the Beacon Bay CPF in the East London area. Face-to-face in-depth individual interviews were used to gather in-depth data. The manual method of analysis was used. The researcher analysed the data by capturing the patterns and direct words of the participants who were interviewed through note taking. Themes were drawn from the responses of the participants and analysed. Some of the findings were that: Community members did not know exactly when the CPF was implemented, the CPF was not effective, community members did not attend CPF meetings and had negative attitudes towards CPF, the police worked better with the rich people rather than the poor ones and there were some influences of politics in the CPF. In addition, the resources lacking at the CPF were: SAPS staff, shortage of police stations and insufficient vehicles at the police stations. Furthermore, some police worked hand-iniv hand with the criminals and the repercussions of poor trust between the community and the police included: community members taking the law into their own hands, poor handling of criminal cases, and escalation of crime in the community. Some community members noted the positive effect that the implementation of the community police forum had as they could now engage the police directly on matters relating to crime and how they think such challenges can be addressed. The researcher made some recommendations towards an effective implementation of the Community Police Forum in Beacon Bay in East London.
- Full Text:
- Authors: Twani, Sydney Vuyisile
- Date: 2019
- Subjects: Community policing -- South Africa Crime prevention Police-community relations Police -- South Africa
- Language: English
- Type: Thesis , Masters , MPA
- Identifier: http://hdl.handle.net/10353/11031 , vital:36687
- Description: The purpose of this study was to investigate the extent to which the Beacon Bay Community Police Forum in the East London area has implemented the Community Police Forum (CPF) uniform constitution. The main research question of this study was: To what extent has the Beacon Bay Community Police Forum in the East London area implemented the uniform Community Police Forum (CPF) constitution? The theoretical framework that guided this study was the broken windows theory. The literature review looked into the following: existing knowledge based on the assessment of the implementation of the uniform CPF constitution by the Beacon Bay Community Police Forum, the regulatory framework and an overview of the role and functions of the community police forum (CPF) in South Africa, lack of awareness and representativeness as challenges to community police forums effectiveness, inadequate resources in the implementation of an effective community policing forum, lack of trust between the police and the community and the issue of corruption as challenges in the implementation of CPF. The qualitative research method was used in this study. The case study design was also used. The researcher used the purposive sampling technique to select six community members and four community executives of the Beacon Bay CPF in the East London area. Face-to-face in-depth individual interviews were used to gather in-depth data. The manual method of analysis was used. The researcher analysed the data by capturing the patterns and direct words of the participants who were interviewed through note taking. Themes were drawn from the responses of the participants and analysed. Some of the findings were that: Community members did not know exactly when the CPF was implemented, the CPF was not effective, community members did not attend CPF meetings and had negative attitudes towards CPF, the police worked better with the rich people rather than the poor ones and there were some influences of politics in the CPF. In addition, the resources lacking at the CPF were: SAPS staff, shortage of police stations and insufficient vehicles at the police stations. Furthermore, some police worked hand-iniv hand with the criminals and the repercussions of poor trust between the community and the police included: community members taking the law into their own hands, poor handling of criminal cases, and escalation of crime in the community. Some community members noted the positive effect that the implementation of the community police forum had as they could now engage the police directly on matters relating to crime and how they think such challenges can be addressed. The researcher made some recommendations towards an effective implementation of the Community Police Forum in Beacon Bay in East London.
- Full Text:
Challenges of the primigravida’s in accessing antenatal care early and regularly in Buffalo city Metropolitan Municipality, Eastern Cape Province, South Africa
- Seakamela, Khomotso Precious
- Authors: Seakamela, Khomotso Precious
- Date: 2019
- Subjects: Maternal health services Prenatal care
- Language: English
- Type: Thesis , Masters , MPH
- Identifier: http://hdl.handle.net/10353/16803 , vital:40775
- Description: According to National Department of Health (NDOH National :2015), antenatal care is free in South Africa’s public health system and nearly all pregnant women and girls attend an antenatal clinic at least once during their pregnancy. However, most pregnant women do not access antenatal care until the later stage of pregnancy. Such delays have been linked to nearly a quarter of avoidable maternal deaths in South Africa. According to World Health Organisation (WHO, 2016; 11), maternal mortality and morbidity are some of the most important global health issues facing the world today. Worldwide, approximately 1000 women die each day from pregnancy and childbirth related causes (WHO, 2016; 11). In addition, 99% of these maternal deaths occur in the developing world, with sub-Saharan Africa accounting for over half of these deaths. The international community has committed to improving maternal health by 2015 with Millennium Development Goal (MDG) number five, which aimed to reduce maternal mortality by three quarters and reach universal access to reproductive health care (www.worldbank.org/mdgs/). Even with this commitment, many countries like South Africa have failed to implement effective programs to reduce maternal mortality and morbidity, and women around the world continue to die and suffer from the complications of pregnancy and childbirth. According to NDOH (2015; 2), women who suspect that they might be pregnant should schedule a booking and visit to their clinic and begin antenatal care as soon as possible. These visits include a physical examination, which includes blood pressure checks, weight checks, providing a urine sample, testing for sexually transmitted infections and checking for tuberculosis. Depending on the stage of the pregnancy, healthcare providers may also do blood tests and examination, which includes an ultrasound. The purpose of the study was to to investigate the challenges of Primigravida’s in accessing antenatal care early and regularly in Buffalo City Metropolitan (BCM) and to assist in formulating recommendations for improving factors that are perceived as obstacles to the utilisation of antenatal care services in the BCM District. vii The study was conducted in five facilities at BCM (Dimbaza Community Health Centre, Empilweni Gompo Clinic, Moore Street Clinic, Central Clinic and Notyatyambo Clinic). BCM is one of the seven districts of Eastern Cape Province of South Africa. The seat of BCM is East London. The data was collected using cross-sectional, self-administered questionnaires that were conducted in selected health facilities of Buffalo City Metropolitan. In this study, the population consisted of primigravida’s between the ages of 18-35 who start clinic attendance in the second trimester of pregnancy at five facilities, Buffalo City Metropolitan. Findings: The biggest challenges, as expressed by the participants, were lack of money, unsuitable clinic times, work commitments, delays at clinic and family control. Most of the participants had good experiences of antenatal care. The timing of antenatal clinic attendance is not influenced by age, education, residential zone, employment status and proximity of clinic
- Full Text:
- Authors: Seakamela, Khomotso Precious
- Date: 2019
- Subjects: Maternal health services Prenatal care
- Language: English
- Type: Thesis , Masters , MPH
- Identifier: http://hdl.handle.net/10353/16803 , vital:40775
- Description: According to National Department of Health (NDOH National :2015), antenatal care is free in South Africa’s public health system and nearly all pregnant women and girls attend an antenatal clinic at least once during their pregnancy. However, most pregnant women do not access antenatal care until the later stage of pregnancy. Such delays have been linked to nearly a quarter of avoidable maternal deaths in South Africa. According to World Health Organisation (WHO, 2016; 11), maternal mortality and morbidity are some of the most important global health issues facing the world today. Worldwide, approximately 1000 women die each day from pregnancy and childbirth related causes (WHO, 2016; 11). In addition, 99% of these maternal deaths occur in the developing world, with sub-Saharan Africa accounting for over half of these deaths. The international community has committed to improving maternal health by 2015 with Millennium Development Goal (MDG) number five, which aimed to reduce maternal mortality by three quarters and reach universal access to reproductive health care (www.worldbank.org/mdgs/). Even with this commitment, many countries like South Africa have failed to implement effective programs to reduce maternal mortality and morbidity, and women around the world continue to die and suffer from the complications of pregnancy and childbirth. According to NDOH (2015; 2), women who suspect that they might be pregnant should schedule a booking and visit to their clinic and begin antenatal care as soon as possible. These visits include a physical examination, which includes blood pressure checks, weight checks, providing a urine sample, testing for sexually transmitted infections and checking for tuberculosis. Depending on the stage of the pregnancy, healthcare providers may also do blood tests and examination, which includes an ultrasound. The purpose of the study was to to investigate the challenges of Primigravida’s in accessing antenatal care early and regularly in Buffalo City Metropolitan (BCM) and to assist in formulating recommendations for improving factors that are perceived as obstacles to the utilisation of antenatal care services in the BCM District. vii The study was conducted in five facilities at BCM (Dimbaza Community Health Centre, Empilweni Gompo Clinic, Moore Street Clinic, Central Clinic and Notyatyambo Clinic). BCM is one of the seven districts of Eastern Cape Province of South Africa. The seat of BCM is East London. The data was collected using cross-sectional, self-administered questionnaires that were conducted in selected health facilities of Buffalo City Metropolitan. In this study, the population consisted of primigravida’s between the ages of 18-35 who start clinic attendance in the second trimester of pregnancy at five facilities, Buffalo City Metropolitan. Findings: The biggest challenges, as expressed by the participants, were lack of money, unsuitable clinic times, work commitments, delays at clinic and family control. Most of the participants had good experiences of antenatal care. The timing of antenatal clinic attendance is not influenced by age, education, residential zone, employment status and proximity of clinic
- Full Text:
Challenges on learning and teaching of fraction operations in Grade 6 - a case study in a South African primary school
- Authors: Chetty, Ashnee
- Date: 2019
- Subjects: Mathematics -- Study and teaching (Primary) Mathematics -- Study and teaching (Early childhood)
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: http://hdl.handle.net/10353/15869 , vital:40534
- Description: Literature review indicates that world over, fraction operations in primary school are challenging not only for learners but also for teachers and South Africa is not an exception. There have been studies on challenges of fraction operations, but could not locate any study at a Grade 6 level in East London education district. The purpose of this study was to assess the specific strengths and challenges in the learning and teaching of fraction operations in Grade 6 in one of the schools in this district. The research paradigm for this study was post-positivism which adopted a mixed methods approach, utilising both quantitative and qualitative methods. The population consisted of three teachers and 98 Grade 6 learners in one primary school in East London. The learners were under 18 years and in order to comply with South African ethical regulations involving humans, a letter was sent out to all 98 Grade 6 learners’ parents requesting their consent. Only 59 learners brought back the consent letters signed by a parent or legal guardian and hence, the sample consisted of 59 Grade 6 learners. There were two instruments: a survey questionnaire with 15 items for learners and two interview schedules, one for teachers and another for learners. The time to complete the questionnaire was 45 minutes and the time to complete the interviews was less than 20 minutes for learners and 40 minutes for teachers. As part of a pilot study, the questionnaire was given to six learners in 2017 cohort. Also, the questionnaire was given two colleagues teaching Grade 6 from two different schools. Furthermore, two learners from the 2017 cohort and two colleagues from two different schools were interviewed, respectively, to check the validity of the learners’ and teachers’ interview schedules. Based on the feedback, the instruments were modified for use in 2018. The learners were given the questionnaire to answer first before 10 were selected for interviews. Three teachers, one each from Grades 5, 6 and 7 were interviewed. The quantitative data were analysed to lead to a descriptive data presentation. The qualitative data were analysed thematically. It was revealed that there were similarities and differences in the manner in which teachers teach and the challenges they identified. There were similarities and differences among the responses from the learners. They found certain fraction operations challenging and their strengths were similar as well. The common outline of the learners’ strengths were being able to do like-fractions while, their challenges were on doing unlike-fractions. The teachers too concurred with the learners’ views. The learners got confused because they did not understand fractions as part of a whole. The teachers indicated knowledge of more than one teaching method. The teachers also pointed out how times tables is an essential part of being able to do operations of fractions. This study could be beneficial for teachers, learners, parents and curriculum developers. The curriculum developers need to revisit the time frame, the transition between the grades, and how often the concept of fractions appears throughout every term.
- Full Text:
- Authors: Chetty, Ashnee
- Date: 2019
- Subjects: Mathematics -- Study and teaching (Primary) Mathematics -- Study and teaching (Early childhood)
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: http://hdl.handle.net/10353/15869 , vital:40534
- Description: Literature review indicates that world over, fraction operations in primary school are challenging not only for learners but also for teachers and South Africa is not an exception. There have been studies on challenges of fraction operations, but could not locate any study at a Grade 6 level in East London education district. The purpose of this study was to assess the specific strengths and challenges in the learning and teaching of fraction operations in Grade 6 in one of the schools in this district. The research paradigm for this study was post-positivism which adopted a mixed methods approach, utilising both quantitative and qualitative methods. The population consisted of three teachers and 98 Grade 6 learners in one primary school in East London. The learners were under 18 years and in order to comply with South African ethical regulations involving humans, a letter was sent out to all 98 Grade 6 learners’ parents requesting their consent. Only 59 learners brought back the consent letters signed by a parent or legal guardian and hence, the sample consisted of 59 Grade 6 learners. There were two instruments: a survey questionnaire with 15 items for learners and two interview schedules, one for teachers and another for learners. The time to complete the questionnaire was 45 minutes and the time to complete the interviews was less than 20 minutes for learners and 40 minutes for teachers. As part of a pilot study, the questionnaire was given to six learners in 2017 cohort. Also, the questionnaire was given two colleagues teaching Grade 6 from two different schools. Furthermore, two learners from the 2017 cohort and two colleagues from two different schools were interviewed, respectively, to check the validity of the learners’ and teachers’ interview schedules. Based on the feedback, the instruments were modified for use in 2018. The learners were given the questionnaire to answer first before 10 were selected for interviews. Three teachers, one each from Grades 5, 6 and 7 were interviewed. The quantitative data were analysed to lead to a descriptive data presentation. The qualitative data were analysed thematically. It was revealed that there were similarities and differences in the manner in which teachers teach and the challenges they identified. There were similarities and differences among the responses from the learners. They found certain fraction operations challenging and their strengths were similar as well. The common outline of the learners’ strengths were being able to do like-fractions while, their challenges were on doing unlike-fractions. The teachers too concurred with the learners’ views. The learners got confused because they did not understand fractions as part of a whole. The teachers indicated knowledge of more than one teaching method. The teachers also pointed out how times tables is an essential part of being able to do operations of fractions. This study could be beneficial for teachers, learners, parents and curriculum developers. The curriculum developers need to revisit the time frame, the transition between the grades, and how often the concept of fractions appears throughout every term.
- Full Text:
Characterization and antibiogram fingerprints of Enterobacteriaceae and Listeria monocytogenes in irrigation water and agricultural soil samples collected from Amathole and Chris Hani District Municipalities in the Eastern Cape Province, South Africa
- Authors: Iwu, Chidozie Declan
- Date: 2019
- Subjects: Listeria monocytogenes Enterobacteriaceae
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/17187 , vital:40864
- Description: Characterization and antibiogram fingerprints of Enterobacteriaceae and Listeria monocytogenes in irrigation water and agricultural soil samples collected from Amathole and Chris Hani District Municipalities in the Eastern Cape Province, South Africa
- Full Text:
- Authors: Iwu, Chidozie Declan
- Date: 2019
- Subjects: Listeria monocytogenes Enterobacteriaceae
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/17187 , vital:40864
- Description: Characterization and antibiogram fingerprints of Enterobacteriaceae and Listeria monocytogenes in irrigation water and agricultural soil samples collected from Amathole and Chris Hani District Municipalities in the Eastern Cape Province, South Africa
- Full Text:
Characterization of the heat source of thermal aquifers within the Soutpansberg Basin in the Limpopo Province, South Africa: Evidence from geophysical and geological investigations
- Authors: Nyabeze, Peter Kushara
- Date: 2019
- Subjects: Basins (Geology) -- Analysis Geology, Structural -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD (Geology)
- Identifier: http://hdl.handle.net/10353/15167 , vital:40192
- Description: The research was conducted to contribute towards the knowledge base on the potential for geothermal energy in the Soutpansberg Basin, located in the Limpopo Province of South Africa. The focus area was Siloam, an area that hosts a hottest spring with the highest recorded temperature of 67.5°C. The research involved visits to the Soutpansberg Basin, water sampling, carrying out ground geophysical surveys, and high-level processing of airborne magnetic data to determine depths and temperatures of magnetic sources. The water samples from the hot springs were found to be enriched in sodium, bicarbonate and chlorine with very low concentrations of other element species. The chemical composition of the spring water indicated a source chemistry comprising of the Na-ClHCO3 water assemblage that is a typical signature for deep circulating groundwater of meteoric origin. The circulation depth was inferred to be 2.0 km. The increased resolution of the ground magnetic, electrical resistivity tomography, and electromagnetic conductivity methods data made it possible to delineate subsurface structures at the spring such as dykes, sills, faults and fractures from generated depth models. Modelling of ground magnetic data showed that the Siloam hot spring occurred between two interpreted north dipping dykes approximately 150 m apart. The minimum depth extent of the dykes was interpreted to be 650 m. The magnetic susceptibility values determined from rock measurements and modelling of magnetic data indicated the presence of volcanic and metamorphic rocks. Electromagnetic profiling data showed that there were three main high conductivity zones in the study area with values above 100 mS/m; A central zone associated with the spring; A zone to the south of the spring and a north zone associated with the Siloam Fault. Ground geophysics survey results confirmed the existence of the Siloam Fault. Two artesian boreholes with water warmer than 40 °C were identified to the south of the Siloam hot spring. Both electromagnetic conductivity and electrical resistivity tomography surveys delineated lateral and vertical variation in the bedrock to depths of 40 m to 60 m. Water bearing structures that could be faults, or fractures were identified. Layering due to weathering and water content was found to be in the depth range of 20 m to 40 m. The depths of the potential heat sources were computed from the radially averaged power spectrum of airborne magnetic data for square blocks with side dimensions L of 51 km, 103 km, and 129 km. Spectral analysis based approaches namely Centroid method, Spectral peak method, and the Fractal based approach were used for computing depth and temperatures to heat sources. Airborne magnetic data sets with larger window sizes were preferred for depth computations, as they preserved spectral signatures of deeper sources and reduced the contribution of shallower sources. The size of the data windows did not have a marked effect of depth and temperature values. Shallower magnetic sources depths of approximately 2.0 km were delineated using the Euler deconvolution method. An anticlinal feature at depths of 2.0 to 4.5 km was 4 Final Submission of Thesis, Dissertation or Research Report/Project, Conference or Exam Paper delineated in the central part of the basin. Spectral analysis results indicated that the depth to the top of magnetic sources was at 3.5 km to 6.2 km; the centroid of the basement at 7.92 km to 13.41 km, and the basal below 11.09 km and 14.08 km. The lower end depth spectrum was determined from application of the Centroid method with the deeper being results from the Fractal based approach. The Spectral peak method was useful for determining the depth to the top of magnetic sources. The temperature of the top of magnetic sources and basement centroid were computed to be in the range 234.00 °C to 281.34 °C. Magnetic source depths and basal temperatures that were in the Curie point range within which rocks lose magnetism due to heat were determined, using a computation approach that utilised fractal parameters, to be 21.39 km and 577.42 °C, respectively. Increasing the value of the fractal parameter β from 0 to 4, had an effect of retaining deeper depths and higher temperatures. The fractal parameter β range of 3 to 4 that gave the Curie point parameters indicated basal rock types with an igneous predisposition. The research highlighted evidence for the existence of the Soutpansberg Basin Geothermal Field (SBGF). The area around Siloam is a potential target for drilling exploration geothermal energy boreholes based on the occurrence of hot springs, shallow heat source depths, anticlinal structure, high formation temperatures, deep circulating water and the achieved Curie point temperature.
- Full Text:
- Authors: Nyabeze, Peter Kushara
- Date: 2019
- Subjects: Basins (Geology) -- Analysis Geology, Structural -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD (Geology)
- Identifier: http://hdl.handle.net/10353/15167 , vital:40192
- Description: The research was conducted to contribute towards the knowledge base on the potential for geothermal energy in the Soutpansberg Basin, located in the Limpopo Province of South Africa. The focus area was Siloam, an area that hosts a hottest spring with the highest recorded temperature of 67.5°C. The research involved visits to the Soutpansberg Basin, water sampling, carrying out ground geophysical surveys, and high-level processing of airborne magnetic data to determine depths and temperatures of magnetic sources. The water samples from the hot springs were found to be enriched in sodium, bicarbonate and chlorine with very low concentrations of other element species. The chemical composition of the spring water indicated a source chemistry comprising of the Na-ClHCO3 water assemblage that is a typical signature for deep circulating groundwater of meteoric origin. The circulation depth was inferred to be 2.0 km. The increased resolution of the ground magnetic, electrical resistivity tomography, and electromagnetic conductivity methods data made it possible to delineate subsurface structures at the spring such as dykes, sills, faults and fractures from generated depth models. Modelling of ground magnetic data showed that the Siloam hot spring occurred between two interpreted north dipping dykes approximately 150 m apart. The minimum depth extent of the dykes was interpreted to be 650 m. The magnetic susceptibility values determined from rock measurements and modelling of magnetic data indicated the presence of volcanic and metamorphic rocks. Electromagnetic profiling data showed that there were three main high conductivity zones in the study area with values above 100 mS/m; A central zone associated with the spring; A zone to the south of the spring and a north zone associated with the Siloam Fault. Ground geophysics survey results confirmed the existence of the Siloam Fault. Two artesian boreholes with water warmer than 40 °C were identified to the south of the Siloam hot spring. Both electromagnetic conductivity and electrical resistivity tomography surveys delineated lateral and vertical variation in the bedrock to depths of 40 m to 60 m. Water bearing structures that could be faults, or fractures were identified. Layering due to weathering and water content was found to be in the depth range of 20 m to 40 m. The depths of the potential heat sources were computed from the radially averaged power spectrum of airborne magnetic data for square blocks with side dimensions L of 51 km, 103 km, and 129 km. Spectral analysis based approaches namely Centroid method, Spectral peak method, and the Fractal based approach were used for computing depth and temperatures to heat sources. Airborne magnetic data sets with larger window sizes were preferred for depth computations, as they preserved spectral signatures of deeper sources and reduced the contribution of shallower sources. The size of the data windows did not have a marked effect of depth and temperature values. Shallower magnetic sources depths of approximately 2.0 km were delineated using the Euler deconvolution method. An anticlinal feature at depths of 2.0 to 4.5 km was 4 Final Submission of Thesis, Dissertation or Research Report/Project, Conference or Exam Paper delineated in the central part of the basin. Spectral analysis results indicated that the depth to the top of magnetic sources was at 3.5 km to 6.2 km; the centroid of the basement at 7.92 km to 13.41 km, and the basal below 11.09 km and 14.08 km. The lower end depth spectrum was determined from application of the Centroid method with the deeper being results from the Fractal based approach. The Spectral peak method was useful for determining the depth to the top of magnetic sources. The temperature of the top of magnetic sources and basement centroid were computed to be in the range 234.00 °C to 281.34 °C. Magnetic source depths and basal temperatures that were in the Curie point range within which rocks lose magnetism due to heat were determined, using a computation approach that utilised fractal parameters, to be 21.39 km and 577.42 °C, respectively. Increasing the value of the fractal parameter β from 0 to 4, had an effect of retaining deeper depths and higher temperatures. The fractal parameter β range of 3 to 4 that gave the Curie point parameters indicated basal rock types with an igneous predisposition. The research highlighted evidence for the existence of the Soutpansberg Basin Geothermal Field (SBGF). The area around Siloam is a potential target for drilling exploration geothermal energy boreholes based on the occurrence of hot springs, shallow heat source depths, anticlinal structure, high formation temperatures, deep circulating water and the achieved Curie point temperature.
- Full Text:
Communication barriers within virtual communities in an ethnoreligious diverse society: a case study of Nigeria
- Authors: Ogunnubi ,Adeyemi
- Date: 2019
- Subjects: Communication
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10353/15859 , vital:40533
- Description: The study explores the communication barriers that exist within virtual communities in an ethno-religious diverse society like Nigeria. Qualitative research approach was employed. Subsequently, data was collected using qualitative questionnaire that contained open ended questions and through participant observation (online ethnography). Purposive sampling was used to select participants through liaising with Facebook users, as one of the online virtual communities in Nigeria. Having monitored online discourse on Facebook for some months, this study purposively selected 300 active facebook users from various Nigerian cultural and ethnic backgrounds, but only 60 participants indicated their interest to participate. Also, out of the 60 participants who agreed to participate, only 30 participants (25 males and 5 females) fully participated to the final stage while the other 30 withdrew from the study. The participants were mainly adults between the age brackets of 20 and 59. Thematic Analysis was subsequently used to analyse the data by identifying main themes to provide detailed descriptions of the setting, participants as well as activities. The findings of this study revealed that majority of the participants agree that due to Facebook familiarity and Nigeria's religious colouration, there is a display of disrespect, insolence and deep arrogance to other interactants online. The study further identified and revealed other barriers such as differences in cultural backgrounds and opinions, lack of politeness, prejudging and filtering, name calling and insults. The study proposes that social media platforms as virtual communities should be regulated with full implementation of the law and its sustenance, regardless of individuals having power to make their own choices about the kind of languages they use within virtual communities and considering its effect on other online users. Vulgar languages, hate speeches, incitements, bullying and other offensive comments would hopefully be curbed to the barest minimum if this intervention could be implemented. The conclusion of the study was that Nigerians should be civil in responding to discourse in virtual communities as certain inflammatory, religious or utterances based on ethnicity due to an uninformed perspective usually display one's poor knowledge on issues of national interests
- Full Text:
- Authors: Ogunnubi ,Adeyemi
- Date: 2019
- Subjects: Communication
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10353/15859 , vital:40533
- Description: The study explores the communication barriers that exist within virtual communities in an ethno-religious diverse society like Nigeria. Qualitative research approach was employed. Subsequently, data was collected using qualitative questionnaire that contained open ended questions and through participant observation (online ethnography). Purposive sampling was used to select participants through liaising with Facebook users, as one of the online virtual communities in Nigeria. Having monitored online discourse on Facebook for some months, this study purposively selected 300 active facebook users from various Nigerian cultural and ethnic backgrounds, but only 60 participants indicated their interest to participate. Also, out of the 60 participants who agreed to participate, only 30 participants (25 males and 5 females) fully participated to the final stage while the other 30 withdrew from the study. The participants were mainly adults between the age brackets of 20 and 59. Thematic Analysis was subsequently used to analyse the data by identifying main themes to provide detailed descriptions of the setting, participants as well as activities. The findings of this study revealed that majority of the participants agree that due to Facebook familiarity and Nigeria's religious colouration, there is a display of disrespect, insolence and deep arrogance to other interactants online. The study further identified and revealed other barriers such as differences in cultural backgrounds and opinions, lack of politeness, prejudging and filtering, name calling and insults. The study proposes that social media platforms as virtual communities should be regulated with full implementation of the law and its sustenance, regardless of individuals having power to make their own choices about the kind of languages they use within virtual communities and considering its effect on other online users. Vulgar languages, hate speeches, incitements, bullying and other offensive comments would hopefully be curbed to the barest minimum if this intervention could be implemented. The conclusion of the study was that Nigerians should be civil in responding to discourse in virtual communities as certain inflammatory, religious or utterances based on ethnicity due to an uninformed perspective usually display one's poor knowledge on issues of national interests
- Full Text:
Competencies of Qualified Primary Health Care professional nurses in Assessing, Diagnosing and Managing clients in health facilities in Buffalo City Metro”
- Authors: Falati, Patience Yoliswa
- Date: 2019
- Subjects: Primary health care
- Language: English
- Type: Thesis , Masters/Doctoral , MPH
- Identifier: http://hdl.handle.net/10353/16716 , vital:40766
- Description: The study investigated the competencies of qualified PHC professional nurses in assessing, diagnosing and managing clients in the facilities. Aim: The study aimed to explain the competences of qualified PHC professional nurses in assessing, diagnosing and managing clients in the facilities. The research study followed a descriptive and qualitative design. Data was collected from qualified PHC professional nurses in 79 clinics (both urban and rural), 4CHC’s and 4 hospital casualties. A structured questionnaire with closed ended questions was used. Data analysis was done by means of descriptive statistics. The study showed that the overall percentage of 90% denotes that these PHC professional nurses are competent in all the aspects of competences. Assessing competences of qualified PHC professional nurses in assessing, diagnosing and managing clients in the facilities is of vital importance for quality health care/ improving service delivery and client satisfaction. Recommendation were made to influence the implementation of policies and guidelines for quality service delivery
- Full Text:
- Authors: Falati, Patience Yoliswa
- Date: 2019
- Subjects: Primary health care
- Language: English
- Type: Thesis , Masters/Doctoral , MPH
- Identifier: http://hdl.handle.net/10353/16716 , vital:40766
- Description: The study investigated the competencies of qualified PHC professional nurses in assessing, diagnosing and managing clients in the facilities. Aim: The study aimed to explain the competences of qualified PHC professional nurses in assessing, diagnosing and managing clients in the facilities. The research study followed a descriptive and qualitative design. Data was collected from qualified PHC professional nurses in 79 clinics (both urban and rural), 4CHC’s and 4 hospital casualties. A structured questionnaire with closed ended questions was used. Data analysis was done by means of descriptive statistics. The study showed that the overall percentage of 90% denotes that these PHC professional nurses are competent in all the aspects of competences. Assessing competences of qualified PHC professional nurses in assessing, diagnosing and managing clients in the facilities is of vital importance for quality health care/ improving service delivery and client satisfaction. Recommendation were made to influence the implementation of policies and guidelines for quality service delivery
- Full Text:
Computational fluid dynamics design and performance testing of a cyclone heat exchanger used in a gasification plant
- Authors: George, Tomy
- Date: 2019
- Subjects: Computational fluid dynamics Renewable energy sources Biomass gasification
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/19087 , vital:39877
- Description: Renewable energy resources are considered to be the most promising source of energy in the coming centuries owing to its potential to get replenished by the nature itself. However, there are a number of challenges that are to be addressed prior to considering a particular resource as a reliable and effective one according to the energy demand fluctuations. Energy extracted from the biomass available in the nature has a lot of potential especially in the case of producing energy from syngas produced by the controlled burning of wood. This research deals with the minimisation of energy wastage in a gasification plant where syngas is produced from wood and transferred to an internal combustion engine to further generate electrical energy. The syngas produced from the wood is to be fed through a cyclone for purification before it can be fed into the IC engine, where the dust, carbon and other impurities are separated from the syngas by making use of the centrifugal force developed inside the cyclone. This syngas is further cooled and fed into the IC engine in its purest form as specified by the requirements of the engine. It is envisaged that if it is possible to recover the heat energy otherwise wasted in the cyclone and through the cooling circuit, it can be used for preheating the wood or for other minor heating purposes, and thereby the heat wastage can be minimised. Therefore, in this research, a series of numerical investigation conducted which involved the modelling of heat exchanger around a cyclone in order to extract heat from the surface of the cyclone. Two basic design of the Heat exchanger were considered in the study, a rectangular and a conical design. With a water flow rate of 2 l/min in conical design, the heat absorbed by the water was found to be 5.555 kJ/s and in rectangular design, the heat absorbed by the water was 4.872 kJ/s. An experimental analysis conducted to validate the simulation by a rectangular Heat exchanger shows the amount of heat absorbed by the water is 4.87 kJ/s. In a study with variable water flow rate through the jacket, it is observed that heat absorbed by the water in conical heat exchanger is more than heat absorbed by the water in rectangular jacket within the flow rate of 4 l/min. and the heat absorbed by the water in rectangular heat exchanger is higher than conical heat exchanger with water flow rate above 4 l/min.
- Full Text:
- Authors: George, Tomy
- Date: 2019
- Subjects: Computational fluid dynamics Renewable energy sources Biomass gasification
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/19087 , vital:39877
- Description: Renewable energy resources are considered to be the most promising source of energy in the coming centuries owing to its potential to get replenished by the nature itself. However, there are a number of challenges that are to be addressed prior to considering a particular resource as a reliable and effective one according to the energy demand fluctuations. Energy extracted from the biomass available in the nature has a lot of potential especially in the case of producing energy from syngas produced by the controlled burning of wood. This research deals with the minimisation of energy wastage in a gasification plant where syngas is produced from wood and transferred to an internal combustion engine to further generate electrical energy. The syngas produced from the wood is to be fed through a cyclone for purification before it can be fed into the IC engine, where the dust, carbon and other impurities are separated from the syngas by making use of the centrifugal force developed inside the cyclone. This syngas is further cooled and fed into the IC engine in its purest form as specified by the requirements of the engine. It is envisaged that if it is possible to recover the heat energy otherwise wasted in the cyclone and through the cooling circuit, it can be used for preheating the wood or for other minor heating purposes, and thereby the heat wastage can be minimised. Therefore, in this research, a series of numerical investigation conducted which involved the modelling of heat exchanger around a cyclone in order to extract heat from the surface of the cyclone. Two basic design of the Heat exchanger were considered in the study, a rectangular and a conical design. With a water flow rate of 2 l/min in conical design, the heat absorbed by the water was found to be 5.555 kJ/s and in rectangular design, the heat absorbed by the water was 4.872 kJ/s. An experimental analysis conducted to validate the simulation by a rectangular Heat exchanger shows the amount of heat absorbed by the water is 4.87 kJ/s. In a study with variable water flow rate through the jacket, it is observed that heat absorbed by the water in conical heat exchanger is more than heat absorbed by the water in rectangular jacket within the flow rate of 4 l/min. and the heat absorbed by the water in rectangular heat exchanger is higher than conical heat exchanger with water flow rate above 4 l/min.
- Full Text: